Horticulture Research最新文献

{"title":"Establishment of a Sonication-Assisted AGL1-Mediated Transformation and Gene-Editing System in Ornamental\u0000 Lilium\u0000 Species","authors":"Weiwei Bao,Hao Liu,Yufan Zhou,Dexuan Ma,Jie Yin,Chunjie Wei,Qingyun Long,Zezhou Ni,Wentao Xu,Kaile Zhu,Xiaoya Jiang,Ying Sun,Minmin Chen,Chong Wang,Hui Zhang","doi":"10.1093/hr/uhag422","DOIUrl":"https://doi.org/10.1093/hr/uhag422","url":null,"abstract":"","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"14 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced biomass production by engineering gibberellin signaling in\u0000 Populus","authors":"Tao Yao,Sanskriti Dwivedi,Lindsay P Brown,Rohit Kousika,Natalie G Aldrich,Margot S S Chen,María A Ortega,William P Bewg,Brent Lieb,McKenna Coile,Nancy L Engle,Arthur J Ragauskas,Yunqiao Pu,Tomás Allen Rush,Chung-Jui Tsai,Timothy J Tschaplinski,Gerald A Tuskan,Miaomiao Li,Jin-Gui Chen","doi":"10.1093/hr/uhag423","DOIUrl":"https://doi.org/10.1093/hr/uhag423","url":null,"abstract":"","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"32 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chromosome-level genome assembly and integrated multiomics reveal cucurbitacin B-associated pathogen defense mechanisms in Trichosanthes kirilowii","authors":"Rui Xiong, Zhuannan Chu, Xingxing Peng, Yong Su, Guangsheng Cui, Ying Hu, Kunhua Wei, Ling Dong, Weiwen Li","doi":"10.1093/hr/uhag416","DOIUrl":"https://doi.org/10.1093/hr/uhag416","url":null,"abstract":"Trichosanthes kirilowii is a traditional medicinal herb widely cultivated in China. Among the cultivated varieties, ‘Wanlou 17’, developed by our research group, exhibits significantly higher resistance to pathogen infection. To elucidate the mechanisms underlying this high resistance, we assembled a chromosome-level genome of T. kirilowii (‘Wanlou 17’) using PacBio sequencing technology. The assembly size is 4.75 Gb with a contig N50 of 3.69 Mb. A total of 4.37 Gb (92%) genome sequence was anchored to 88 chromosomes, and genotype analysis revealed that it is an autopolyploid octoploid. Metabolome analysis indicated that the high disease resistance of ‘Wanlou 17’ was associated with jasmonic acid (JA) and cucurbitacin B. Experiments demonstrated that exogenous JA promoted the synthesis of cucurbitacin B, and cucurbitacin B enhanced plant resistance to anthracnose. Promoter analysis and co-expression network demonstrated that TkWRKY37 correlated with JA, cucurbitacin B content, as well as biosynthesis pathway genes. Heterologous overexpression experiment confirmed that TkWRKY37 enhanced disease resistance, dual-luciferase showed that TkWRKY37 repressed TkMYC2 expression and activated TkACT1 expression. Furthermore, in vivo silencing of TkWRKY37 in T. kirilowii using antisense oligonucleotide probes significantly reduced the contents of JA and cucurbitacin B, confirming its positive regulatory role. This study provides valuable insights and genomic resources for the molecular breeding research of T. kirilowii.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"9 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149152598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pangenome analysis revealed a receptor-like protein gene that confers bacterial wilt resistance in tomato","authors":"Xiaofen Yu,Huan Liu,Haoen He,Xiaoli Lou,Pan Wu,Shan Li,Sumin Guo,Yong Zhang,Zhangjun Fei,Dousheng Wu,Lei Gao","doi":"10.1093/hr/uhag418","DOIUrl":"https://doi.org/10.1093/hr/uhag418","url":null,"abstract":"","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"10 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149150753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pan-plant phylogenomics reveals ancestral origins, duplication-driven expansion, and conserved regulatory modules of the bZIP transcription factor family","authors":"Xiaocong He, Hao Huang, Yanyan Lu, Zhou Li, Xuechun Zhao, Jihui Chen, Xinyao Gu, Jin He, Chao Chen, Rui Dong","doi":"10.1093/hr/uhag421","DOIUrl":"https://doi.org/10.1093/hr/uhag421","url":null,"abstract":"Basic leucine zipper (bZIP) transcription factors represent one of the largest and most conserved transcription factor families in plants, yet their evolutionary origin, expansion dynamics, and regulatory diversification across the plant kingdom remain incompletely understood. Here, we identified 22,378 bZIP genes from 321 representative plant species spanning major lineages from red algae to angiosperms and integrated phylogenomic reconstruction, duplication-mode analysis, evolutionary constraint assessment, comparative transcriptomics, regulatory-network inference, pangenome analysis, and functional validation. Phylogenetic analyses revealed that plant bZIP proteins are organized into four major clades comprising 13 conserved subgroups, and that the core subgroup architecture was established before the emergence of land plants. Family expansion showed strong lineage dependence, with dispersed duplication predominating in early-diverging lineages and whole-genome/segmental duplication becoming the major expansion mode in angiosperms. Despite extensive expansion during plant evolution, pervasive purifying selection maintained the structural conservation of bZIP proteins, particularly within the conserved bZIP domain. Comparative expression and regulatory network analyses revealed substantial divergence in bZIP regulatory programs across plant lineages. AtbZIP27 and MsbZIP26 activated their corresponding MIOX promoters, whereas SlbZIP70 did not, indicating that the bZIP–MIOX regulatory relationship is conserved in some lineages but has diverged in others. Functional assays under aluminum stress further supported the biological relevance of this regulatory relationship, while tomato pan-genome analysis revealed that such regulatory divergence can occur against a largely conserved genomic background. Collectively, these findings reveal that plant bZIP evolution combines duplication-driven expansion and strong selective constraint with progressive diversification of regulatory relationships and functions.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"1 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149152600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Regulation of Fruit Development and Aroma Biosynthesis in Durian: Comparative Insights from Tropical Fruit Species","authors":"Muhammad Sajjad, Kang Luo, Yun Zheng","doi":"10.1093/hr/uhag424","DOIUrl":"https://doi.org/10.1093/hr/uhag424","url":null,"abstract":"Durian (Durio zibethinus L.) is a commercially important tropical fruit characterized by climacteric ripening and a distinctive sulfur-rich aroma. Recent advances in genomics, transcriptomics, and metabolomics have provided valuable insights into the molecular mechanisms underlying fruit development and aroma biosynthesis in durian. Comparative evidence from mango (Mangifera indica L.), banana (Musa spp.), papaya (Carica papaya L.), and jackfruit (Artocarpus heterophyllus Lam.) indicates that hormonal and transcriptional mechanisms involved in fruit ripening share conserved features among these species, whereas aroma-related metabolic pathways differ among species. However, the regulatory connections among developmental processes, hormonal signaling, transcriptional regulation, and metabolic reprogramming that determine aroma formation remain insufficiently integrated. This review focuses on the molecular networks connecting fruit development, hormonal signaling, transcription factor regulation, and metabolic reprogramming during aroma formation in durian. Particular emphasis is placed on sulfur-containing volatiles and methionine catabolism, which contribute to the characteristic aroma of durian. Comparative evidence from these tropical fruits are used to distinguish conserved mechanisms of climacteric ripening from species-specific mechanisms of volatile metabolism. The roles of ethylene biosynthesis and signaling, transcription factors, and key biosynthetic genes involved in fruit development and aroma formation are also discussed. Postharvest changes in volatile compounds and emerging approaches, including genome editing and metabolic engineering, are further discussed for their potential to improve aroma quality and breeding efficiency. This review provides an integrated molecular framework linking developmental regulation, hormone signaling, transcription factors, metabolic pathways, and aroma-associated phenotypes in durian, while highlighting conserved and species-specific mechanisms across tropical fruits.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"53 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149152603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"bZIP17 modulates pedicel fruit abscission zone formation in sweet cherry","authors":"Michail Michailidis, Christina Skodra, Evangelia Mouchtaropoulou, Vaia Styliani Titeli, Eleni Giannoutsou, Georgia Tanou, Ioannis-Dimosthenis S Adamakis, Anagnostis Argiriou, Christos Bazakos, Athanassios Molassiotis","doi":"10.1093/hr/uhag426","DOIUrl":"https://doi.org/10.1093/hr/uhag426","url":null,"abstract":"Pedicel-fruit abscission zone formation determines fruit detachment and directly affects the feasibility of mechanical harvesting in sweet cherry, yet its molecular regulation remains insufficiently understood. Here, we combined physiological, anatomical, metabolomic, and transcriptomic analysis to investigate pedicel-fruit abscission zone development in two cultivars with contrasting detachment phenotypes, ‘Tragana Edessis’ (low pedicel retention force) and ‘Tsolakeika’ (high pedicel retention force). The abscission-prone cultivar exhibited distinct structural and metabolic features at the pedicel-fruit junction, including enhanced pectin remodeling, altered cell wall composition, and accumulation of cell wall-derived sugars, consistent with localized cell wall disassembly. Transcriptome analysis revealed extensive reprogramming of genes involved in cell wall modification, carbohydrate metabolism, and hormone-related pathways, and identified the endoplasmic reticulum-associated transcription factor PavbZIP17 as a candidate regulator. RNA interference-mediated silencing of PavbZIP17 in two abscission-resistant cultivars (‘Tsolakeika’ and ‘Bakirtzeika’) decreased pedicel detachment force and enhanced pedicel-fruit abscission zone development. Transcriptomic analysis following PavbZIP17 silencing identified differentially expressed genes associated with vesicular trafficking (PavPRA1), phospholipid signaling (PavPLD1), cell wall glycosylation (PavGALT6), and hormone signaling (PavARF2B). Transient promoter transactivation assays showed that a subset of the tested promoter fragments responded to PavbZIP17, supporting the corresponding genes as promoter-responsive candidates. These results suggest that PavbZIP17 participates in the regulatory network underlying pedicel-fruit abscission zone development, providing insights into fruit abscission in sweet cherry and identifying candidate targets for breeding strategies aimed at improving mechanical harvesting.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"31 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149152602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of Resistance and Susceptibility to Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4) in Banana: A Review","authors":"Zijie Huang, Qiusong Deng, Tangxin Li, Peng Ling, Rony Swennen, Jingyang Li","doi":"10.1093/hr/uhag425","DOIUrl":"https://doi.org/10.1093/hr/uhag425","url":null,"abstract":"Banana (Musa spp.) is a globally critical staple food and cash crop, yet its production is threatened by Fusarium wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), a soil-borne pathogen with a broad host range and persistent virulence. This review synthesizes current knowledge of Foc TR4’s infection process, host immune responses, molecular mechanisms of resistance, pathogen evasion strategies, and breeding approaches for durable resistance. We highlight key findings from multi-omics studies, synthetic biology applications, and regulatory considerations for field deployment. Critical knowledge gaps include the molecular basis of Foc TR4’s broad host compatibility, the role of epigenetic modifications in resistance, and the long-term stability of transgenic resistance in diverse agroecosystems. Addressing these gaps is essential for developing sustainable management strategies to safeguard banana production against Foc TR4.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"8 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149152601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A PbrNAC14 activated\u0000 PbrMYBL4/PbrSGR2\u0000 repressor modulates pigment homeostasis in pear fruit pericarp","authors":"Shangyun Li,Yutao Yang,Liyun Sui,Yichi Wang,Weiqi Wang,Yunting Zhang,Yuanxiu Lin,Qing Chen,Ya Luo,Haoru Tang,Yong Zhang","doi":"10.1093/hr/uhag419","DOIUrl":"https://doi.org/10.1093/hr/uhag419","url":null,"abstract":"Abstract The concurrent processes of anthocyanin accumulation and chlorophyll degradation are pivotal to fruit color development and quality attainment during ripening. However, the regulatory mechanisms that coordinate their dynamic equilibrium within the fruit pericarp remain poorly understood. In this study, we identified the NAC transcription factor PbrNAC14 as a key regulator by examining the distinctive red-to-yellow-green transition zone of 'Red Zaosu' pear pericarp. Our results demonstrated that PbrNAC14 could repress anthocyanin biosynthesis by activating the transcriptional repressor PbrMYBL4 via direct binding to the TTTCGTGT and TTTCGT cis-elements within the PbrMYBL4 promoter. Concurrently, PbrNAC14 modulated chlorophyll degradation by binding to the ACGAAA and CGTG elements in the promoter of the chlorophyll catabolism gene PbrSGR2. In summary, our data establish that PbrNAC14 directly activates PbrMYBL4 and PbrSGR2, which act as repressors of anthocyanin and chlorophyll turnover, respectively, thereby fine-tuning pericarp pigmentation during pear fruit ripening. These findings uncover a previously unknown regulatory network and identify potential genetic targets for precision breeding programs to enhance fruit quality.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"24 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149087983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutation of a conserved leucine residue generates a dominant-negative movement protein that confers resistance to tomato brown rugose fruit virus in tomato","authors":"Meng-Yuan Yan,Xiu-Qi Mu,Yan-Li Zhang,Yan-Ting Liu,Yi-Heng Yue,Xiao-Xue Huan,Hua-Yu Ma,Yong-Guang Wang,Chao Geng,Jun Jiang,Yan-Ping Tian,Jing-Chun Wu,Zhi-Yong Yan,Xiang-Dong Li","doi":"10.1093/hr/uhag415","DOIUrl":"https://doi.org/10.1093/hr/uhag415","url":null,"abstract":"","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"8 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149087980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}