Horticulture Research最新文献_第4页

Warming temperature reduces the risk of pre-harvest freezing injury and modifies variety suitability in the main winegrape-growing regions of China 在中国主要的葡萄产区，温度升高降低了收获前冻害的风险，改变了品种的适宜性

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-07 DOI: 10.1093/hr/uhaf176

Huiqing Bai, Jianqiang He, Cornelis Leeuwen, Rafiq Hamdi, Erna Blancquaert, Gregory V Jones, Zhanwu Dai

{"title":"Warming temperature reduces the risk of pre-harvest freezing injury and modifies variety suitability in the main winegrape-growing regions of China","authors":"Huiqing Bai, Jianqiang He, Cornelis Leeuwen, Rafiq Hamdi, Erna Blancquaert, Gregory V Jones, Zhanwu Dai","doi":"10.1093/hr/uhaf176","DOIUrl":"https://doi.org/10.1093/hr/uhaf176","url":null,"abstract":"Rising temperatures cause advanced phenology of grapevines and increased sugar concentration in berries, which ultimately modify variety suitability in a given region. Here, four bioclimatic indices and a refined Grapevine Sugar Ripeness (GSR) model were employed to assess the suitability of six winegrape varieties across six winegrape-growing regions of China under historical climate conditions (1961-2020). First, four indices were compared between two periods, one before (P1) and one after (P2) an abrupt climate change events identified during 1988-2002 in these regions. Results showed three temperature related indices increased in six regions while the first fall frost day (FFD) was delayed by 0-16 days in five out of the six regions during P2 compared with P1. Second, GSR model was applied to simulate target sugar concentrations as a proxy for grape harvest dates (GHDs). Direct utilization of original GSR model yielded unsatisfactory predictions with clear bias. Consequently, GSR model was recalibrated with local data to obtain an acceptable performance with R2 and NRMSE values of 0.83 and 2.8% as well as 0.83 and 3.1% for the calibration and validation datasets, respectively and further simulated GHDs of six varieties with advanced values of 6-30 days in six regions for P2 in comparison with P1. To provide a holistic view of freezing injury risk before harvest, comprehensive freezing injury index (CFI) was developed by integrating the frequency, duration, and severity of the freezing risk. CFI decreased (2%-60%) during P2 in all regions and the magnitudes of decrease was elevation dependent. These findings provide valuable insights for the selection of varieties that can more reliably achieve fully mature fruit, producing more balanced wines with greater typicity under warming climate.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"31 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144568571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding Steroid-Derived Metabolite Engineering in Solanum 解读茄属植物类固醇衍生代谢物工程

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-07 DOI: 10.1093/hr/uhaf178

Farah Kanwal, Sana Ullah, Yingchen Hao, Shouchuang Wang

{"title":"Decoding Steroid-Derived Metabolite Engineering in Solanum","authors":"Farah Kanwal, Sana Ullah, Yingchen Hao, Shouchuang Wang","doi":"10.1093/hr/uhaf178","DOIUrl":"https://doi.org/10.1093/hr/uhaf178","url":null,"abstract":"Steroidal glycoalkaloids (SGAs) and steroidal saponins (STSs) play significant role in the plant defense against pests and offer various pharmaceuticals applications. SGAs and STSs generally share common biosynthetic pathways in Solanum, originating from a furostanol scaffold. Despite the discovery of multiple GLYCOALKALOID METABOLISM (GAME) genes involved in the biosynthesis of these compounds, previous attempts for the metabolic engineering of these pathways have remained unsuccessful. The GAME15 protein, with its dual enzymatic roles, has unlocked a mystery surrounding the intricate process of metabolizing cholesterol. This protein not only acts as a glucuronosyltransferase but also serves as a metabolic scaffold, organizing several proteins for the proper functioning. This mini review briefly describes the molecular mechanisms and functional dynamics of GAME genes, particularly focusing on GAME15 as a key game changer gene and its role in metabolite channeling, regulation of pathway, and ecological importance. We highlighted the potential of this discovery for advancing metabolic engineering in crop improvement and the pharmaceutical industry. This finding opens doors for designing crops that are resistant to pests. Additionally, we identify important future research directions, including the regulatory mechanisms of these pathways and uncovering structural aspects of pivotal enzymes.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"2 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144568572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omics analyses reveal the effects of layerage and grafting on flavonoid synthesis and accumulation in Citrus reticulata ‘Chachi’ 多组学分析揭示了分层和嫁接对‘茶柑’黄酮合成和积累的影响

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-07 DOI: 10.1093/hr/uhaf177

Jianmu Su, Mingmin Jiang, Huimin Pan, Weitao Zhou, Xueyan Cai, Yukun Wang, Wei Liu, Desen Wang, Mei Bai, Hong Wu

{"title":"Multi-omics analyses reveal the effects of layerage and grafting on flavonoid synthesis and accumulation in Citrus reticulata ‘Chachi’","authors":"Jianmu Su, Mingmin Jiang, Huimin Pan, Weitao Zhou, Xueyan Cai, Yukun Wang, Wei Liu, Desen Wang, Mei Bai, Hong Wu","doi":"10.1093/hr/uhaf177","DOIUrl":"https://doi.org/10.1093/hr/uhaf177","url":null,"abstract":"Guangdong Citri Reticulatae Pericarpium from the dry and mature peel of Citrus reticulata ‘Chachi’ (CRC) is a well-known medicinal and food materials in Asia. The main propagation methods of CRC are layerage and grafting. It is generally considered that the quality of CRC from layerage is superior to that obtained from plants propagated by grafting. Nevertheless, the effects of layerage and grafting on main bioactive ingredients flavonoid biosynthesis in peel of CRC keep unknown. Here, metabolomic analyses revealed the effects of layerage, self-grafting and heterografting (C. limonia as rootstock) on flavonoid biosynthesis in CRC from two main harvesting periods, CRCV (Citri Reticulatae Chachiensis Viride) and CRCR (Citri Reticulatae Chachiensis Reddish). Compared with CRCR, CRCV exhibited a higher content of flavonoids. Grafting CRC onto C. limonia exhibited a higher content of hesperidin, nobiletin, tangeretin, narirutin, demethylnobiletin and sinensetin, than layerage and self-grafting. This increase can be attributed to the upregulation of genes involved in flavonoid synthesis. Further, the transcription factor CrcMYBF1 was identified within the gene co-expression network and is confirmed to be significantly induced by methyl jasmonate (MeJA) and up-regulate the expression of Crc1,6RhaT through interacting with its promoter region, thereby boosting the biosynthesis and accumulation of hesperidin. In summary, our findings provide mechanistic insights into the coordinated regulation of hesperidin biosynthesis via MeJA inducing CrcMYBF1 in CRC. Our study is expected to provide a theoretical basis for CRC propagation and cultivation.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"81 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144578466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide association studies revealed partial genetic links between early vigor and precocity in macadamia 全基因组关联研究揭示了澳洲坚果早期活力和早熟之间的部分遗传联系

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-04 DOI: 10.1093/hr/uhaf162

Pragya Dhakal Poudel, Joanne-De Faveri, Bruce Topp, Mobashwer Alam

{"title":"Genome-wide association studies revealed partial genetic links between early vigor and precocity in macadamia","authors":"Pragya Dhakal Poudel, Joanne-De Faveri, Bruce Topp, Mobashwer Alam","doi":"10.1093/hr/uhaf162","DOIUrl":"https://doi.org/10.1093/hr/uhaf162","url":null,"abstract":"Early vigor (EV) and precocity are important traits for orchard establishment and profitability in macadamia. EV determines tree growth and adaptation, while precocity facilitates early yield, offering economic benefits. Although, a positive relationship between these traits has been observed in other tree crops, their association in macadamia remains unclear. This study aimed to identify genetic links between EV and precocity by assessing genetic variability, heritability and correlations in a five-year-old macadamia breeding population. The population comprised 904 progenies planted across six sites in Queensland, Australia. Genome-wide association studies (GWAS) were conducted on a subset of 220 accessions genotyped with 7,401 SNP markers. A linear mixed model incorporating a kinship matrix and principal components to account for population structure was used to perform association analysis in TASSEL. Phenotypic analyses in ASReml-R revealed that precocity had higher broad- (H2 = 0.25-0.84) and narrow-sense (h2 = 0.10-0.77) heritability compared to EV (H2 = 0-0.61, h2 = 0-0.49). EV and precocity showed positive phenotypic (0.25-0.42) and genetic (0.21-0.31) correlations. GWAS identified 11 significant markers (FDR &lt;0.05), including two pleiotropic markers (Mint10079 and Mint4004) associated with both EV and precocity. Putative genes linked to these markers were involved in cell wall modelling, pathogen defence, abiotic stress tolerance, flowering, overall growth and development in other tree species. These significant markers, post-validation, hold substantial promise for utilization in marker-assisted selection (MAS). Integrating putative pleiotropic markers into MAS can enhance genetic gain by reducing the selection time for and enabling simultaneous selection for EV and precocity.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"50 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144565961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterization of key enzymes involved in the biosynthesis of distinctive flavonoids and stilbenoids in Morus notabilis 桑中黄酮类化合物和苯乙烯类化合物合成关键酶的功能研究

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-04 DOI: 10.1093/hr/uhaf171

Meng-Wen Hu, Jie Fu, Ying Lu, Xin-Yan Liu, Jiao-Zhen Zhang, Jiang-Nan Li, Dan-Dan Xu, Ya-Tong Li, Pei-Xi Hao, Ming-Xin Cui, Lin-Lin Zhi, Hong-Xiang Lou, Ai-Xia Cheng

{"title":"Functional characterization of key enzymes involved in the biosynthesis of distinctive flavonoids and stilbenoids in Morus notabilis","authors":"Meng-Wen Hu, Jie Fu, Ying Lu, Xin-Yan Liu, Jiao-Zhen Zhang, Jiang-Nan Li, Dan-Dan Xu, Ya-Tong Li, Pei-Xi Hao, Ming-Xin Cui, Lin-Lin Zhi, Hong-Xiang Lou, Ai-Xia Cheng","doi":"10.1093/hr/uhaf171","DOIUrl":"https://doi.org/10.1093/hr/uhaf171","url":null,"abstract":"The mulberry (Morus notabilis) is a medicinal and edible plant and contains diverse flavonoids and stilbenoids with significant medicinal benefits. The biosynthesis of these compounds has only been partially elucidated. In the present investigation, we identified and characterized two 4-coumarate: coenzyme A ligases (Mn4CL1 and Mn4CL2), two polyketide synthases (MnCHS and MnSTS), three chalcone reductases (MnCHR1-3) and two 2-oxoglutarate-dependent dioxygenases (MnFLS and MnF3H) involved in flavonoids and stilbenoids biosynthesis. MnCHS converts p-coumaroyl-CoA into naringenin and facilitates the novel conversion of 2,4-dihydroxycinnamoyl-CoA to steppogenin, which features hydroxyl groups at the 4' and 6' positions on the B ring. MnSTS could convert p-coumaroyl-CoA and 2,4-dihydroxycinnamoyl-CoA into resveratrol and oxyresveratrol, respectively. Furthermore, MnCHR1 was firstly identified in mulberry and collaborated with MnCHS to produce isoliquiritigenin and 2,4,2',4'-tetrahydroxychalcone. A co-expression system of Mn4CL1, MnCHS and MnCHR1 enabled the fermentation production of steppogenin and 2,4,2',4'-tetrahydroxychalcone in engineered Escherichia coli. The in vitro enzymatic assays confirmed that MnFLS showed both FLS and F3H activities, whereas transgenic experiments revealed its predominant FLS function in vivo; MnF3H was confirmed as a bona fide F3H. These findings provide new insights into the flavonoids and stilbenoids biosynthesis pathway in mulberry and suggest a potential parallel pathway for 4',6'-dihydroxylated flavonoids biosynthesis.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"103 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144568576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mango Pangenome Reveals Dramatic Impacts of Reference Bias on Genomic Analyses 芒果泛基因组揭示了参考偏差对基因组分析的巨大影响

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-01 DOI: 10.1093/hr/uhaf166

Bilal Ahmad, Ying Su, Yani Hao, Tayyaba Razzaq, Rida Arshad, Yi Zhang, Yingchun Zhang, Xingyi Wang, Guizhou Huang, Xiangnian Su, Ting Hou, Chaochao Li, Xuanwen Yang, Chuanning Li, Zhenzhou Chu, Qiuyan Wang, Yu Zhang, Zhongxin Jin, Qi Xu, Xiaodong Xu, Yanling Peng, Guiqi Bi, Chengjie Chen, Yeyuan Chen, Hua Xiao, Jianfeng Huang, Yongfeng Zhou, Xinmin Tian

{"title":"Mango Pangenome Reveals Dramatic Impacts of Reference Bias on Genomic Analyses","authors":"Bilal Ahmad, Ying Su, Yani Hao, Tayyaba Razzaq, Rida Arshad, Yi Zhang, Yingchun Zhang, Xingyi Wang, Guizhou Huang, Xiangnian Su, Ting Hou, Chaochao Li, Xuanwen Yang, Chuanning Li, Zhenzhou Chu, Qiuyan Wang, Yu Zhang, Zhongxin Jin, Qi Xu, Xiaodong Xu, Yanling Peng, Guiqi Bi, Chengjie Chen, Yeyuan Chen, Hua Xiao, Jianfeng Huang, Yongfeng Zhou, Xinmin Tian","doi":"10.1093/hr/uhaf166","DOIUrl":"https://doi.org/10.1093/hr/uhaf166","url":null,"abstract":"Most genomics studies start by mapping sequencing data to a reference genome. The quality of reference genome assembly, genetic relatedness to the studied population, and the mapping method employed directly impact variant calling accuracy and subsequent genomic analyses, introducing reference bias and resulting in erroneous conclusions. However, the impacts of reference bias and methods to reduce it have gained limited attention. This study compared genomic analyses using four different reference genomes of mango (Mangifera indica), including the two haploid assemblies of haplotype-resolved telomere-to-telomere (T2T) genome assembly, a pangenome, and an older version of the reference genome available on NCBI. The choice of reference genome dramatically impacted the mapping efficiency and resulted in notable differences in calling the genetic variants, particularly structural variations (SVs). Phylogenetic analysis was more sensitive to the reference genome compared to genetic differentiation. Population genomic analyses of artificial selection in domestication and SV hotspot regions varied across reference genomes. Notably, the gene enrichment analyses showed significant differences in the top enriched biological processes depending on the reference genome used. Overall, the mango pangenome outperformed the other reference genomes across various metrics, followed by T2T reference genomes, as they captured greater diversity and effectively reduced reference bias. Our findings highlight the role of the mango pangenome in reducing reference bias and underscore the critical role of reference genome selection, suggesting that it is one of the most important factors in genomic studies.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"77 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144533121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A NAC family gene PmNAC32 associated with photoperiod promotes flower induction in Prunus mume 一个与光周期相关的NAC家族基因PmNAC32促进了李的花诱导

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-06-29 DOI: 10.1093/hr/uhaf157

Chengdong Ma, Pengyu Zhou, Yufan Ma, Wei Tan, Xiao Huang, Silas Segbo, Shahid Iqbal, Ting Shi, Zhaojun Ni, Zhihong Gao

{"title":"A NAC family gene PmNAC32 associated with photoperiod promotes flower induction in Prunus mume","authors":"Chengdong Ma, Pengyu Zhou, Yufan Ma, Wei Tan, Xiao Huang, Silas Segbo, Shahid Iqbal, Ting Shi, Zhaojun Ni, Zhihong Gao","doi":"10.1093/hr/uhaf157","DOIUrl":"https://doi.org/10.1093/hr/uhaf157","url":null,"abstract":"The photoperiod is essential to flower induction, and the exact timing of the process can be precisely regulated based on the relative duration of light and darkness. However, the mechanisms linking photoperiod and flower induction in woody plants remain largely unexplored. Using RNA-seq, we identified a photoperiod response factor PmNAC32, which is predominantly expressed in early-flowering varieties. Overexpression of PmNAC32 in Arabidopsis thaliana, tobacco and Prunus mume calli resulted in accelerated flowering. Binding and activation analyses revealed that PmNAC32 can be directly suppressed by REVEILLE 1 (RVE1) and REVEILLE 3 (RVE3), implying that PmNAC32 plays a role in the photoperiodic signaling pathway. Further studies established that PmNAC32 functions as a positive regulator of CONSTANS-LIKE 5 (COL5) and a negative regulator of CONSTANS-LIKE 4 (COL4). Interestingly, we identified two homologs of PmNAC32, namely PmNAC29 and PmNAC47. These three proteins can interact with each other and enhance the regulation of PmCOL4 and PmCOL5. Although PmNAC29 and PmNAC47 can promote flower induction respectively, neither of them responded to the photoperiod. Thus, our results reveal a novel mechanism by which PmNAC32 regulates flower induction in Prunus mume.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"1 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Essential Gene Dynamics across Distinct Growth Conditions in Xanthomonas citri 柑橘黄单胞菌在不同生长条件下的基本基因动力学

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-06-28 DOI: 10.1093/hr/uhaf160

Weiwei Lv, Xiaolan Wang, Yi Tan, Junan Zhu, Yifei Ge, Yunfei Wang, Manying Wu, Meirui Song, Yu Zhang, Yue Shen, Jihua Wang, Yunzeng Zhang, Lei Li, Zhigang Li, Jinfang Tan, Feng Luo, Lingling Zheng, Xiaofeng Zhou

{"title":"Essential Gene Dynamics across Distinct Growth Conditions in Xanthomonas citri","authors":"Weiwei Lv, Xiaolan Wang, Yi Tan, Junan Zhu, Yifei Ge, Yunfei Wang, Manying Wu, Meirui Song, Yu Zhang, Yue Shen, Jihua Wang, Yunzeng Zhang, Lei Li, Zhigang Li, Jinfang Tan, Feng Luo, Lingling Zheng, Xiaofeng Zhou","doi":"10.1093/hr/uhaf160","DOIUrl":"https://doi.org/10.1093/hr/uhaf160","url":null,"abstract":"Essential genes are crucial for bacterial growth and the pathogenicity. However, how gene essentiality varies under distinct growth conditions and its role in virulence remains poorly understood. In this study, we constructed high-saturation Tn5 transposon mutant libraries for Xanthomonas citri subsp. citri (Xac), the causative agent of citrus canker disease, which results in substantial economic losses to the citrus industry. These libraries were generated under two growth conditions: nutrient-rich medium (NB) and plant-mimicking medium (XVM2). The libraries achieved an average insertion density of 6.85 base pairs, ensuring high-quality coverage. Using these libraries, we identified 568 genes in both bacterial populations as essential genes of Xac, while the 61 (NB) and 54 (XVM2) as conditional essential genes. To validate the findings, two allele-replacement mutants, ∆ublA and ∆pgl, were generated based on variations in insertion density and gap ratios between two growth conditions. These mutants exhibited significant growth defects in XVM2 and compromised virulence in citrus plants, as evidenced by weaker canker symptoms and reduced bacterial populations in planta. Additionally, we performed a comparative analysis of essential gene conservation across the Xanthomonas genus and other species within the class Gammaproteobacteria. A pan-essentialome dataset was assembled from four Xanthomonas strains, revealing a broad pan-essentialome and a precise core-essentialome. To facilitate further research, we developed XanthoBrowser ( http://xacdb.lumoxuan.cn/), an online resource that provides user-friendly interface for searching and comparison of essential genes across four Xanthomonas strains. In conclusion, this study provides comprehensive insights into bacterial adaptation mechanisms and highlight potential targets for bactericide development.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"70 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CrWRKY57 and CrABF3 cooperatively activate CrCYCD6;1 to modulate drought tolerance and root development CrWRKY57和CrABF3协同激活CrCYCD6；1，调节根系的耐旱性和发育

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-06-28 DOI: 10.1093/hr/uhaf158

Jinxia Mo, Xinting Xiong, Zaofa Zhong, Lu Liu, Ying Xiong, Min Wang, Wenshan Dai, Shaohua Zeng, Ting Peng

{"title":"CrWRKY57 and CrABF3 cooperatively activate CrCYCD6;1 to modulate drought tolerance and root development","authors":"Jinxia Mo, Xinting Xiong, Zaofa Zhong, Lu Liu, Ying Xiong, Min Wang, Wenshan Dai, Shaohua Zeng, Ting Peng","doi":"10.1093/hr/uhaf158","DOIUrl":"https://doi.org/10.1093/hr/uhaf158","url":null,"abstract":"Drought is a major abiotic stress. WRKYs are one of the largest family of transcription factors in plant. The effects of most WRKYs on developmental regulation and drought adaptation in Citrus remain largely unclear. C. reticulata cv. Sanhu hongju (Sanhu) is a drought-tolerant variety from Jiangxi Province, China. Here, we report a differentially expressed CrWRKY57 gene in drought-treated Sanhu leaves through transcriptome analysis. Its transcriptional expression could be induced by ABA treatment and water deficit. Overexpression of CrWRKY57 in lemon (C. limon) and tobacco (Nicotiana tabacum) confers enhanced drought tolerance, while RNA interference (RNAi)-mediated silencing in Sanhu increases dehydration susceptibility and reduces root volume. Moreover, virus-induced gene silencing (VIGS)-mediated knockdown of CrWRKY57 in Sanhu reduces primary root length and lateral root number by nearly 50% compared to the control. The results of yeast two-hybrid, co-immunoprecipitation assays, and bimolecular fluorescence complementation demonstrate that CrWRKY57 interacts with CrABF3, a key transcription factor in ABA signaling. Silencing ClABF3, its homolog in lemon, also increases drought sensitivity and disrupts root system development. Together, CrWRKY57 and CrABF3 directly activate the promoter of the cell cycle gene CrCYCD6;1 by binding to W-box and ABRE elements, respectively. Furthermore, silencing CrCYCD6;1 in Sanhu also severely reduces primary root length and lateral root number. Collectively, our findings provide a new perspective of CrWRKY57 as a positive player in drought response and highlight the role of the CrWRKY57-CrABF-CrCYCD6;1 module in enhancing drought tolerance by modulating root development.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"45 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OVATE family gene CmOFP6-19b negatively regulates fruit size in melon (Cucumis melo L.) OVATE家族基因CmOFP6-19b负调控甜瓜果实大小

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-06-27 DOI: 10.1093/hr/uhaf148

Junling Chi, Haimei Yan, Wenjing Zhang, Dingfang Tian, Gen Che, Agula Hasi

{"title":"OVATE family gene CmOFP6-19b negatively regulates fruit size in melon (Cucumis melo L.)","authors":"Junling Chi, Haimei Yan, Wenjing Zhang, Dingfang Tian, Gen Che, Agula Hasi","doi":"10.1093/hr/uhaf148","DOIUrl":"https://doi.org/10.1093/hr/uhaf148","url":null,"abstract":"OVATE family proteins (OFPs) constitute a class of transcription factors regulating various developmental processes in plants. Nevertheless, their precise regulatory functions in melon (Cucumis melo L.) fruit development remain elusive. In this study, we identified expression profiling of melon OFP genes and revealed the molecular function of CmOFP6-19b gene mediating fruit size variation. Quantitative analysis revealed predominant CmOFPs expression in reproductive organs (female/male flowers and ovaries), with distinct differential expression patterns observed among paralogs. Through melon genetic transformation, we revealed that CmOFP6-19b gene functions as a negative regulator in fruit enlargement. Overexpression of the CmOFP6-19b gene resulted in reduced fruit size, while its downregulation led to increased fruit size. Bimolecular fluorescence complementation (BiFC) and yeast two-hybrid (Y2H) assays confirmed nuclear-localized physical interaction between CmOFP6-19b and CmKNOX16. Overexpression of CmKNOX16 in melon produced smaller fruits, phenocopying the CmOFP6-19b-Oe lines. RT-qPCR analysis showed negative correlation between CmOFP6-19b/CmKNOX16 expression level and fruit size, with peak expression levels observed in a cultivar displaying minimal longitudinal diameter. The results of histological section and expression analysis suggest that CmOFP6-19b and CmKNXO16 may affect melon fruit size by regulating genes related to cell division and cell expansion. In conclusion, our findings systematically characterized the phylogenetic architecture and expression divergence of CmOFP genes, and elucidated the function and molecular mechanism of CmOFP6-19b-CmKNOX16 regulatory module in mediating melon fruit development, providing a theoretical foundation for melon breeding.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"9 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144500551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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