Horticulture Research最新文献_第4页

A single base mutation in promoter of CsTPR enhances the negative regulation on mechanical related leaves droopiness in tea plant CsTPR启动子单碱基突变增强了茶树叶片机械相关下垂的负调控作用

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-29 DOI: 10.1093/hr/uhaf098

Haoran Liu, Lingxiao Duan, Chaqin Tang, Jianqiang Ma, Ji-Qiang Jin, Jiedan Chen, Weizhong He, Mingzhe Yao, Liang Chen

{"title":"A single base mutation in promoter of CsTPR enhances the negative regulation on mechanical related leaves droopiness in tea plant","authors":"Haoran Liu, Lingxiao Duan, Chaqin Tang, Jianqiang Ma, Ji-Qiang Jin, Jiedan Chen, Weizhong He, Mingzhe Yao, Liang Chen","doi":"10.1093/hr/uhaf098","DOIUrl":"https://doi.org/10.1093/hr/uhaf098","url":null,"abstract":"Mechanical harvesting in the tea industry has become increasingly essential due to its advantages in increasing productivity and reducing labor costs. Leaves droopiness caused a high rate of broken leaves, hindering the mechanized harvesting quality. However, the underlying mechanisms remain unclear. We herein identified a quantitative trait loci, designated as q10.3, along with three lead SNPs located near a TPR gene (TETRATRICOPEPTIDE REPEAT), named CsTPR, through performing a genome-wide association study (GWAS) on 130 tea accessions. Integrated analysis of RNA-seq and ATAC-seq confirmed CsTPR as a droopiness-associated candidate gene at the transcriptional level. CsTPR was then proved to negatively regulate brassinosteroid -induced droopiness by using the CsTPR-silencing tea plant. The whole genome sequencing (WGS) and genome walking cloning further indicated that a single base mutation (T to A) in the promoter of CsTPR. ChIP-seq revealed that this mutation occurred within the binding site, E-box, of CsBES1.2 on the CsTPR promoter. Notably, CsBES1.2 bound the E-box of CsTPR promoter to repress the expression of CsTPR, as demonstrated by ChIP-qPCR, electrophoretic mobility shift assays (EMSA), and transient assays. The single base mutation strengthened the inhibitory effect of CsBES1.2 on the expression of CsTPR via enhancing the binding affinity to the E-box. Altogether, our findings suggest that CsTPR negatively regulates droopiness in tea plants under the transcriptional repression of CsBES1.2 and that a single base mutation within E-box amplifies the suppression of CsBES1.2 on the expression of CsTPR.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"211 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The SmWRKY32-SmbHLH65/SmbHLH85 regulatory module mediates tanshinone biosynthesis in Salvia miltiorrhiza SmWRKY32-SmbHLH65/SmbHLH85调控模块介导丹参酮在丹参中的生物合成

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-28 DOI: 10.1093/hr/uhaf096

Xiumin Nie, Xueying Li, Bingbing Lv, Shuai Shao, Bin Zhang, Juane Dong

{"title":"The SmWRKY32-SmbHLH65/SmbHLH85 regulatory module mediates tanshinone biosynthesis in Salvia miltiorrhiza","authors":"Xiumin Nie, Xueying Li, Bingbing Lv, Shuai Shao, Bin Zhang, Juane Dong","doi":"10.1093/hr/uhaf096","DOIUrl":"https://doi.org/10.1093/hr/uhaf096","url":null,"abstract":"Tanshinones are valuable compounds found in Salvia miltiorrhiza, and gaining a deeper understanding of their transcriptional regulation mechanisms is a key strategy for increasing their content. Previous research revealed that SmWRKY32 acts as a repressor of tanshinone synthesis. This study identified the SmbHLH65 transcription factor, whose expression was significantly reduced in the SmWRKY32 overexpression transcriptome. Overexpression of SmbHLH65 stimulated tanshinone accumulation, while its silencing resulted in a decrease in tanshinone content. However, SmbHLH65 does not directly target the key enzyme genes involved in tanshinone synthesis. Subsequently, we discovered the SmbHLH65-interacting protein SmbHLH85. SmbHLH85 facilitates tanshinone biosynthesis by directly upregulating SmDXS2 and SmCPS1. Further investigation demonstrated that SmbHLH65 not only promotes the expression of SmbHLH85 but also enhances its binding to the promoters of SmDXS2 and SmCPS1, thereby amplifying the activation of these biosynthetic genes. Additionally, SmWRKY32 directly binds to the SmbHLH65 promoter to suppress its activity. In summary, these findings reveal that the regulatory module SmWRKY32-SmbHLH65/SmbHLH85 controls tanshinone synthesis in S. miltiorrhiza. This study uncovers a novel transcriptional regulatory mechanism, offering fresh insights into the complex network controlling tanshinone biosynthesis.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"74 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic prediction and association analyses for breeding parthenocarpic blueberries 用于培育孤雌生殖蓝莓的基因组预测和关联分析

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-23 DOI: 10.1093/hr/uhaf086

Juliana Cromie, Ryan P Cullen, Camila Ferreira Azevedo, Luis Felipe V Ferrão, Felix Enciso-Rodriguez, Juliana Benevenuto, Patricio R Muñoz

{"title":"Genomic prediction and association analyses for breeding parthenocarpic blueberries","authors":"Juliana Cromie, Ryan P Cullen, Camila Ferreira Azevedo, Luis Felipe V Ferrão, Felix Enciso-Rodriguez, Juliana Benevenuto, Patricio R Muñoz","doi":"10.1093/hr/uhaf086","DOIUrl":"https://doi.org/10.1093/hr/uhaf086","url":null,"abstract":"Parthenocarpy is a desirable trait that enables fruit set in the absence of fertilization. While blueberries typically depend on pollination for optimal yield, certain genotypes can produce seedless fruits through facultative parthenocarpy, eliminating the need for pollination. However, the development of parthenocarpic cultivars has remained limited by the challenge of evaluating large breeding populations. Thus, establishing molecular breeding tools can greatly accelerate genetic gain for this trait. In the present study, we evaluated two blueberry breeding populations for parthenocarpic fruit set and performed genome-wide association studies (GWAS) to identify markers and candidate genes associated with parthenocarpy. We also compared the predictive ability (PA) of three molecular breeding approaches, including i) genomic selection (GS); ii) GS de novo GWAS (GSdnGWAS), which incorporates significant GWAS markers into the GS model as prior information; and iii) in-silico marker-assisted selection (MAS), where markers from GWAS were fitted as fixed effects with no addition marker information. GWAS analyses identified 55 marker-trait associations, revealing candidate genes related to phytohormones, cell cycle regulation, and seed development. Predictive analysis showed that GSdnGWAS consistently outperformed GS and MAS, with PAs ranging from 0.21 to 0.36 depending on the population of study and the specific markers utilized. MAS showed PAs comparable to GS in some cases, suggesting it could be a cost-effective alternative to genome-wide sequencing. Together, these findings demonstrate that molecular breeding techniques can be used to improve facultative parthenocarpy, offering new avenues to develop high-yielding blueberry varieties that are less reliant on pollination.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"3 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VaMIEL1-Mediated Ubiquitination of VaMYB4a Orchestrates Cold Tolerance through Integrated Transcriptional and Oxidative Stress Pathways in Grapevine VaMIEL1 介导的 VaMYB4a 泛素化通过综合转录和氧化应激途径协调葡萄的耐寒能力

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-23 DOI: 10.1093/hr/uhaf093

Yaping Xie, Kai Lv, Qinhan Yu, Jieping Wu, Junxia Zhang, Huixian Zhao, Junduo Li, Ningbo Zhang, Weirong Xu

{"title":"VaMIEL1-Mediated Ubiquitination of VaMYB4a Orchestrates Cold Tolerance through Integrated Transcriptional and Oxidative Stress Pathways in Grapevine","authors":"Yaping Xie, Kai Lv, Qinhan Yu, Jieping Wu, Junxia Zhang, Huixian Zhao, Junduo Li, Ningbo Zhang, Weirong Xu","doi":"10.1093/hr/uhaf093","DOIUrl":"https://doi.org/10.1093/hr/uhaf093","url":null,"abstract":"Cold stress poses a significant threat to viticulture, particularly under the increasing pressures of climate change. In this study, we identified VaMIEL1, a RING-type E3 ubiquitin ligase from Vitis amurensis, as a negative regulator of cold tolerance. Under normal temperature conditions, VaMIEL1 facilitates the ubiquitination and subsequent proteasomal degradation of the cold-responsive transcription factor VaMYB4a, thereby attenuating its regulatory role in the CBF-COR signaling cascade. However, under cold stress, VaMIEL1 expression is downregulated, leading to the stabilization of VaMYB4a and the activation of CBF-COR signaling.Through a combination of biochemical assays and functional analysis in Arabidopsis thaliana and grapevine calli, we demonstrate that VaMIEL1 overexpression reduces cold tolerance, as evidenced by increased oxidative stress, excessive ROS accumulation, and downregulated expression of cold-responsive genes. Conversely, silencing of VaMIEL1 enhances cold tolerance by stabilizing VaMYB4a and boosting antioxidant defenses. These findings uncover a previously unrecognized regulatory mechanism by which VaMIEL1 modulates cold tolerance through transcriptional and oxidative stress pathways, offering potential targets for the development of climate-resilient grapevine cultivars and other crops.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"220 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ZjMYB44-ZjPOD51 module enhances jujube defence response against phytoplasma by upregulating lignin biosynthesis ZjMYB44-ZjPOD51模块通过上调木质素的生物合成来增强红枣对植物原体的防御反应

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-19 DOI: 10.1093/hr/uhaf083

Liman Zhang, Hongtai Li, Ximeng Wei, Yuanyuan Li, Zhiguo Liu, Mengjun Liu, Weijie Huang, Huibin Wang, Jin Zhao

{"title":"The ZjMYB44-ZjPOD51 module enhances jujube defence response against phytoplasma by upregulating lignin biosynthesis","authors":"Liman Zhang, Hongtai Li, Ximeng Wei, Yuanyuan Li, Zhiguo Liu, Mengjun Liu, Weijie Huang, Huibin Wang, Jin Zhao","doi":"10.1093/hr/uhaf083","DOIUrl":"https://doi.org/10.1093/hr/uhaf083","url":null,"abstract":"Lignin is a major component of the plant cell wall and has a conserved basic defence function in higher plants, helping the plants cope with pathogen infection. However, the regulatory mechanism of lignin biosynthesis in plants under phytoplasma stress remains unclear. In this study, we reported that peroxidase 51 (ZjPOD51), which is involved in lignin monomer polymerization, was induced by phytoplasma infection and that overexpression of ZjPOD51 in phytoplasma-infected jujube seedlings and Arabidopsis plants significantly increased their defence response against phytoplasma. Yeast one-hybrid (Y1H) and luciferase (LUC) assays showed that ZjPOD51 transcription was directly upregulated by ZjMYB44. Genetic validation demonstrated that ZjMYB44 expression was also induced by phytoplasma infection and contributed to lignin accumulation, which consequently enhanced phytoplasma defence in a ZjPOD51-dependent manner. These results demonstrated that the ZjMYB44-ZjPOD51 module enhanced the jujube defence response against phytoplasma by upregulating lignin biosynthesis. Overall, our study first elucidates how plants regulate lignin to enhance their defence response against phytoplasma and provides clues for jujube resistance breeding.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"16 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Panomics-Driven Framework for the Improvement of Major Food Legume Crops: Advances, Challenges, and Future Prospects 主要食用豆类作物改良的泛经济学驱动框架：进展、挑战和未来展望

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-18 DOI: 10.1093/hr/uhaf091

Hongliang Hu, Xingxing Yuan, Dinesh Kumar Saini, Tao Yang, Xinyi Wu, Ranran Wu, Zehao Liu, Farkhandah Jan, Reyazul Rouf Mir, Liu Liu, Jiashun Miao, Na Liu, Pei Xu

{"title":"A Panomics-Driven Framework for the Improvement of Major Food Legume Crops: Advances, Challenges, and Future Prospects","authors":"Hongliang Hu, Xingxing Yuan, Dinesh Kumar Saini, Tao Yang, Xinyi Wu, Ranran Wu, Zehao Liu, Farkhandah Jan, Reyazul Rouf Mir, Liu Liu, Jiashun Miao, Na Liu, Pei Xu","doi":"10.1093/hr/uhaf091","DOIUrl":"https://doi.org/10.1093/hr/uhaf091","url":null,"abstract":"Food legume crops, including common bean, faba bean, mungbean, cowpea, chickpea, and pea, have long served as vital sources of energy, protein, and minerals worldwide, both as grains and vegetables. Advancements in high-throughput phenotyping, next-generation sequencing, transcriptomics, proteomics, and metabolomics have significantly expanded genomic resources for food legumes, ushering research into the panomics era. Despite their nutritional and agronomic importance, food legumes still face constraints in yield potential and genetic improvement due to limited genomic resources, complex inheritance patterns, and insufficient exploration of key traits such as quality and stress resistance. This highlights the need for continued efforts to comprehensively dissect the phenome, genome, and regulome of these crops. This review summarizes recent advances in technological innovations and multi-omics applications in food legumes research and improvement. Given the critical role of germplasm resources and the challenges in applying phenomics to food legumes—such as complex trait architecture and limited standardized methodologies—we first address these foundational areas. We then discuss recent gene discoveries associated with yield stability, seed composition, and stress tolerance and their potential as breeding targets. Considering the growing role of genetic engineering, we provide an update on gene-editing applications in legumes, particularly CRISPR-based approaches for trait enhancement. We advocate for integrating chemical and biochemical signatures of cells ('molecular phenomics') with genetic mapping to accelerate gene discovery. We anticipate that combining panomics approaches with advanced breeding technologies will accelerate genetic gains in food legumes, enhancing their productivity, resilience, and contribution to sustainable global food security.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"61 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A SlRBP1-SlFBA7/SlGPIMT module regulates fruit size in tomato SlRBP1-SlFBA7/SlGPIMT模块调控番茄果实大小

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-18 DOI: 10.1093/hr/uhaf089

Ke Cheng, Duo Lin, Liqun Ma, Yao Lu, Jinyan Li, Guoning Zhu, Tao Lin, Guiqin Qu, Benzhong Zhu, Daqi Fu, Yunbo Luo, Hongliang Zhu

{"title":"A SlRBP1-SlFBA7/SlGPIMT module regulates fruit size in tomato","authors":"Ke Cheng, Duo Lin, Liqun Ma, Yao Lu, Jinyan Li, Guoning Zhu, Tao Lin, Guiqin Qu, Benzhong Zhu, Daqi Fu, Yunbo Luo, Hongliang Zhu","doi":"10.1093/hr/uhaf089","DOIUrl":"https://doi.org/10.1093/hr/uhaf089","url":null,"abstract":"Fleshy fruits are vital to the human diet, providing essential nutrients such as sugars, organic acids, and dietary fibers. RNA-binding proteins play critical functions in plant development and environment adaption, but their specific contributions to fruit development remain largely unexplored. In this study, we centered on the function of SlRBP1 in tomato fruit and reported an unexpected finding that SlRBP1 controls fruit size by regulating its targets SlFBA7 and SlGPIMT. Here, the fruit-specific silencing of SlRBP1 was achieved by artificial miRNA which subsequently led to a marked reduction of fruit size. Cytological analysis suggested that SlRBP1 silencing decreased cell division and expansion of fruit pericarp. Those key genes involved in cell development were significantly repressed in SlRBP1 knock-down mutants. Furthermore, native RNA immunoprecipitation sequencing deciphered 83 SlRBP1-binding target RNAs in fruit, including two targets that are highly expressed in fruit: SlFBA7 and SlGPIMT, which are involved in developing fruit. Indeed, silencing either SlFBA7 or SlGPIMT resulted in fruit size reduction identical to that seen with SlRBP1 silencing. These results suggest that SlRBP1 modulates fruit size through its targets SlFBA7 and SlGPIMT. Our findings provide novel perspectives on the molecular mechanisms though which RNA-binding proteins control fruit size.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"13 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BrRCO promotes leaf lobe formation by repressing BrACP5 expression in Brassica rapa BrRCO 通过抑制 BrACP5 在甘蓝中的表达来促进叶裂的形成

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-12 DOI: 10.1093/hr/uhaf084

Yunxia Sun, Limin Hu, Junrey C Amas, William J W Thomas, Lihui Wang, Xian Wang, Wei Wang, Gaoyang Qu, Xiaoxiao Shen, Ruiqin Ji, Jacqueline Batley, Chuchuan Fan, Yugang Wang

{"title":"BrRCO promotes leaf lobe formation by repressing BrACP5 expression in Brassica rapa","authors":"Yunxia Sun, Limin Hu, Junrey C Amas, William J W Thomas, Lihui Wang, Xian Wang, Wei Wang, Gaoyang Qu, Xiaoxiao Shen, Ruiqin Ji, Jacqueline Batley, Chuchuan Fan, Yugang Wang","doi":"10.1093/hr/uhaf084","DOIUrl":"https://doi.org/10.1093/hr/uhaf084","url":null,"abstract":"Lobed leaves are advantageous for gas exchange, canopy architecture and high-density planting, however, the genetic mechanisms of leaf lobe formation in Brassica crops remains poorly understood. Here, lob10.1, our previously identified major QTL controlling the presence/absence of leaf lobes in B. rapa (AA), was fine-mapped to a confidence interval of 69.8 kb. REDUCED COMPLEXITY ORGAN (BrRCO, BraA10g032440.3c), a homeodomain leucine zipper class I (HD ZIP I) transcription factor, was predicted to be the most likely candidate gene underlying lob10.1. Null mutations of BrRCO by CRISPR/Cas9 in the lobed-leaf parent RcBr and over-expression in the counter-part near isogenic lines (NILRcBr) leads to entire and lobed leaves, respectively. Analysis of the gene evolution revealed that A10. RCO functions as a core gene and was generally negatively selected in B. rapa. Moreover, BrRCO function as a negative regulator by directly binding to promoters of BrACP5 and repressing its expression. The function of ACID PHOSPHATASE TYPE 5 (BrACP5) was subsequently confirmed as VIGS-BrACP5 produced entire leaves in RcBr. This study identified the core gene BrRCO to be involved in the development of leaf lobes in B. rapa and elucidated a new pathway for leaf lobe formation by the BrRCO-BrACP5 module. These findings provide a theoretical basis for the formation of leaf lobes in Brassica crops.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"49 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salicylic acid and jasmonic acid in plant immunity 水杨酸和茉莉酸在植物免疫中的作用

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-11 DOI: 10.1093/hr/uhaf082

Pingyu Zhang, Edan Jackson, Xin Li, Yuelin Zhang

引用次数: 0

CsWRKY17 enhances Al accumulation by promoting pectin deesterification in tea plant CsWRKY17通过促进果胶脱酯化促进茶树Al积累

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-11 DOI: 10.1093/hr/uhaf085

Danjuan Huang, Jianqiang Ma, Xun Chen, Hongjuan Wang, Rongrong Tan, Long Jiao, Jiedan Chen, Yingxin Mao, Liang Chen

{"title":"CsWRKY17 enhances Al accumulation by promoting pectin deesterification in tea plant","authors":"Danjuan Huang, Jianqiang Ma, Xun Chen, Hongjuan Wang, Rongrong Tan, Long Jiao, Jiedan Chen, Yingxin Mao, Liang Chen","doi":"10.1093/hr/uhaf085","DOIUrl":"https://doi.org/10.1093/hr/uhaf085","url":null,"abstract":"The tea plant (Camellia sinensis) is a typical crop that accumulates aluminum (Al). Although the physiological mechanisms by which this occurs are well understood, their molecular mechanisms remain elusive. Here, an integrative approach combining quantitative trait locus (QTL) mapping of controlled hybridized populations and comparative transcriptomic analysis using samples treated with different Al concentrations was applied to identify candidate genes associated with Al accumulation in tea plants. Consequently, 41 candidate genes were selected using genome functional annotation of the qAl09 locus in the region of 35,256,594–5,737,8817 bp on chromosome 7. Finally, a key gene, CsWRKY17, was identified as encoding a nucleus-localized transcription factor (TF) involved in regulating Al accumulation in tea plants, given the finding of a high correlation between its expression level and Al content in leaves. Overexpression of CsWRKY17 in Arabidopsis increased pectin deesterification, sensitivity to Al stress, and Al accumulation in leaves. Expression of the pectin methylesterase gene CsPME6 was found to be highly consistent with CsWRKY17 expression under various Al concentrations. In addition, experiments using a yeast monoclonal, electrophoresis mobility shift assay, and dual-luciferase reporter system confirmed that CsWRKY17 activated CsPME6 promoter activity. Antisense oligodeoxynucleotide silencing revealed a positive association between CsPME6 expression and Al accumulation in tea shoots. In conclusion, this study suggests that CsWRKY17 promoted the process of pectin deesterification by binding to the CsPME6 promoter, thereby enhancing Al enrichment in tea plants. Our findings lay the foundation for studying the precise mechanisms through which Al enriched in tea leaves.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"16 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143599905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0