Horticulture Research最新文献_第10页

MiR319a-mediated salt stress response in poplar MiR319a 介导的杨树盐胁迫反应

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-06-07 DOI: 10.1093/hr/uhae157

Yanxia Cheng, Qiao Wang, Linxi Yang, Quanzi Li, Xiaojing Yan

{"title":"MiR319a-mediated salt stress response in poplar","authors":"Yanxia Cheng, Qiao Wang, Linxi Yang, Quanzi Li, Xiaojing Yan","doi":"10.1093/hr/uhae157","DOIUrl":"https://doi.org/10.1093/hr/uhae157","url":null,"abstract":"\u0000 Maintenance of intracellular ion balance, especially Na+ and K+, plays an important role in plant responses to salt stress. Vessels in xylem are responsible for long-distance ion transport in vascular plants. Knowledge on the salt stress response in woody plants in limited. In this study, we identified miR319a as an important regulator in respond to salt stress in poplar. miR319a overexpression transgenic poplar showed a salt-tolerant phenotype, and cytological observation showed reduced cambium cell layers, wider xylem, increased number and lumen area of vessels and fibers, and thinner cell wall thickness in the transgenics. While miR319a-MIMIC plants had opposite phenotypes, with narrower xylem, reduced number and lumen area of vessels and fibers cells, and increased wall thickness. In addition, overexpression of miR319a driven by the vessel-specific promoter significantly improved the salt tolerance than by the fiber-specific promoter. The expression levels of PagHKT1;2 and PagSKOR1-b, which encoded high-affinity K+ and Na+ transporters for Na+ efflux and K+ influx, respectively, were positively correlated with the vessel number and lumen area. These results suggest that miR319 not only promotes ion transport rates by increasing vessel number and lumen area and reducing cell wall thickness, but also regulates the concentrations of Na+ and K+ in the xylem by up-regulating PagHKT1;2 and PagSKOR1-b. We demonstrate that miR319 coordinates the response of poplar to salt stress may through both mechanisms, enriching our understanding of the synergistic effects of the secondary xylem structure and long-distance ion transport balance in the salt tolerance of poplar.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141374784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The phosphorylation of a WD40-repeat protein negatively regulates flavonoid biosynthesis in Camellia sinensis under drought stress WD40 重复蛋白的磷酸化负调控干旱胁迫下的山茶花类黄酮生物合成

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-06-03 DOI: 10.1093/hr/uhae136

Zhu Li, Yunyun Han, Xin Li, Jingjuan Zhao, Nana Wang, Yangyang Wen, Tongtong Li, Huangqiang Su, Liping Gao, Tao Xia, Ya-Jun Liu

{"title":"The phosphorylation of a WD40-repeat protein negatively regulates flavonoid biosynthesis in Camellia sinensis under drought stress","authors":"Zhu Li, Yunyun Han, Xin Li, Jingjuan Zhao, Nana Wang, Yangyang Wen, Tongtong Li, Huangqiang Su, Liping Gao, Tao Xia, Ya-Jun Liu","doi":"10.1093/hr/uhae136","DOIUrl":"https://doi.org/10.1093/hr/uhae136","url":null,"abstract":"\u0000 Flavonoids constitute main nutraceuticals in the leaves of tea plants (Camellia sinensis). To date, although it is known that drought stress can negatively impact the biosynthesis of flavonoids in tea leaves, mechanisms behind this phenomenon is unclear. Herein, we report a protein phosphorylation mechanism that negatively regulates the biosynthesis of flavonoids in tea leaves in drought conditions. Transcriptional analysis revealed the downregulation of gene expression of flavonoid biosynthesis and the upregulation of CsMPK4a encoding a mitogen-activated protein kinase in leaves. Luciferase complementation and yeast two-hybrid assays disclosed that CsMPK4a interacted with CsWD40. Phosphorylation assay in vitro, specific protein immunity, and analysis of protein mass spectrometry indicated that Ser-216, Thr-221 and Ser-253 of CsWD40 were potential phosphorylation sites of CsMPK4a. Besides, the protein immunity analysis uncovered an increased phosphorylation level of CsWD40 in tea leaves under drought conditions. Mutation of the three phosphorylation sites generated dephosphorylated CsWD403A and phosphorylated CsWD403D variants, which were introduced into the Arabidopsis ttg1 mutant. Metabolic analysis showed that the anthocyanin and proanthocyanidin content was lower in ttg1:CsWD403D transgenic plants than ttg1::CsWD403A transgenic and wild type plants. The transient overexpression of CsWD403D downregulated the anthocyanidin biosynthesis in tea leaves. The dual-fluorescein protein complementation experiment showed that CsWD403D did not interact with CsMYB5a and CsAN2, two key transcription factors of procyanidins and anthocyanidins biosynthesis in tea plant. These findings indicate that the phosphorylation of CsWD40 by CsMPK4a downregulates the flavonoid biosynthesis in tea plants in drought stresses.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141271574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolome profiling and transcriptome analysis unveiling the crucial role of magnesium transport system for magnesium homeostasis in tea plants 代谢组图谱和转录组分析揭示镁转运系统在茶树镁平衡中的关键作用

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-06-03 DOI: 10.1093/hr/uhae152

Jing Li, Ting Wen, Ruiming Zhang, Xinlong Hu, Fei Guo, Hua Zhao, Pu Wang, Yu Wang, Dejiang Ni, Mingle Wang

{"title":"Metabolome profiling and transcriptome analysis unveiling the crucial role of magnesium transport system for magnesium homeostasis in tea plants","authors":"Jing Li, Ting Wen, Ruiming Zhang, Xinlong Hu, Fei Guo, Hua Zhao, Pu Wang, Yu Wang, Dejiang Ni, Mingle Wang","doi":"10.1093/hr/uhae152","DOIUrl":"https://doi.org/10.1093/hr/uhae152","url":null,"abstract":"\u0000 Magnesium (Mg2+) is a crucial nutrient for the growth and development of Camellia sinensis and is closely related to the quality of tea. However, the underlying mechanisms responding to low-Mg2+ stress in tea plants remain largely unknown. In this study, the photosynthetic parameters, metabolomics, and transcriptomics were utilized to explore the potential effects of low-Mg2+ on the growth and metabolism of C. sinensis. Low-Mg2+ treatment increased the ratio of shoot dry weight to root dry weight but decreased the photosynthesis of C. sinensis. Forty and thirty metabolites were impacted by Mg2+ shortage in C. sinensis shoots and roots, respectively. Integrated transcriptome and metabolome analyses revealed the possible reasons for the decreased contents of chlorophyll (Chl) and catechins and the increased theanine content in C. sinensis roots. Weighted gene co-expression network analysis indicated that Mg2+ transport system was essential in regulation of Mg2+ homeostasis in C. sinensis, in which CsMGT5 was identified to be the key regulator according to CsMGT5-overexpressing and complementary assays in Arabidopsis thaliana. Moreover, silencing of CsMGT5 in vivo reduced the content of Chl in C. sinensis shoots. In addition, CsMGT5 might collaborate with ammonium transporters to keep the steady of amino acids contents, suggesting its potential application for tea quality improvement. All these findings demonstrate the key roles of CsMGTs for Mg2+ homeostasis in C. sinensis, providing theoretical basis for Mg2+ efficient utilization in plants.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141270182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple and efficient gene functional analysis method for studying the growth and development of peach seedlings 研究桃幼苗生长发育的一种简单高效的基因功能分析方法

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-06-03 DOI: 10.1093/hr/uhae155

Jun Cheng, Yun Shao, Xinyue Hu, Liying Gao, Xianbo Zheng, B. Tan, Xia Ye, Wei Wang, Haipeng Zhang, Xiaobei Wang, Xiaodong Lian, Zhiqian Li, Jiancan Feng, Langlang Zhang

{"title":"A simple and efficient gene functional analysis method for studying the growth and development of peach seedlings","authors":"Jun Cheng, Yun Shao, Xinyue Hu, Liying Gao, Xianbo Zheng, B. Tan, Xia Ye, Wei Wang, Haipeng Zhang, Xiaobei Wang, Xiaodong Lian, Zhiqian Li, Jiancan Feng, Langlang Zhang","doi":"10.1093/hr/uhae155","DOIUrl":"https://doi.org/10.1093/hr/uhae155","url":null,"abstract":"\u0000 Stable genetic transformation of peach [Prunus persica (L.) Batsch] still faces many technical challenges, and existing transient expression methods are limited by tissue type or developmental stage, making it difficult to conduct functional analysis of genes regulating shoot growth. To overcome this dilemma, we developed a three-step method for efficient analysis of gene functions during peach seedling growth and development. This method resulted in transformation frequencies ranging from 48 to 87%, depending on the gene. From transformation of germinating seeds to phenotyping of young saplings took just 1.5 months and can be carried out any time of year. To test the applicability of this method, the function of three tree architecture-related genes, namely PpPDS, PpMAX4 and PpWEEP, and two lateral root-related genes, PpIAA14-1 and -2, were confirmed. Since functional redundancy can challenge gene functional analyses, tests were undertaken with the growth-repressor DELLA, which has three homologous genes, PpDGYLA (DG), PpDELLA1 (D1) and -2 (D2), in peach that are functionally redundant. Silencing using a triple-target vector (TRV2-DG-D1-D2) resulted in transgenic plants taller than those carrying just TRV2-DG or TRV2. Simultaneously silencing the three DELLA genes also attenuated the stature of two dwarf genotypes, ‘FHSXT’ and ‘HSX’, which normally accumulate DELLA proteins. Our study provides a method for the functional analysis of genes in peach and can be used for the study of root, stem and leaf development. We believe this method can be replicated in other woody plants.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141272726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chloroplast protein StFC-II was manipulated by a Phytophthora effector to enhance host susceptibility 叶绿体蛋白 StFC-II 受一种 Phytophthora 效应子的操纵，可提高宿主的易感性

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-05-28 DOI: 10.1093/hr/uhae149

Meng Xu, Xinyuan Sun, Xinya Wu, Yetong Qi, Hongjun Li, Jiahui Nie, Zhu Yang, Zhendong Tian

{"title":"Chloroplast protein StFC-II was manipulated by a Phytophthora effector to enhance host susceptibility","authors":"Meng Xu, Xinyuan Sun, Xinya Wu, Yetong Qi, Hongjun Li, Jiahui Nie, Zhu Yang, Zhendong Tian","doi":"10.1093/hr/uhae149","DOIUrl":"https://doi.org/10.1093/hr/uhae149","url":null,"abstract":"Oomycete secretes a range of RxLR effectors into host cells to manipulate plant immunity by targeting proteins from several organelles. In this study, we report that chloroplast protein StFC-II is hijacked by a pathogen effector to enhance susceptibility. Phytophthora infestans RxLR effector Pi22922 is activated during the early stages of P. infestans colonization. Stable overexpression of Pi22922 in plants suppresses flg22-triggered reactive oxygen species (ROS) burst and enhances leaf colonization by P. infestans. A potato ferrochelatase 2 (FC-II, a nuclear-encoded chloroplast-targeted protein), a key enzyme for heme biosynthesis in chloroplast, was identified as a target of Pi22922 in the cytoplasm. The pathogenicity of Pi22922 in plants is partially dependent on FC-II. Overexpression of StFC-II decreased resistance of potato and Nicotiana benthamiana against P. infestans, and silencing of NbFC-II in N. benthamiana reduces P. infestans colonization. Overexpression of StFC-II increases heme content and reduces chlorophyll content and photosynthetic efficiency in potato leaves. Moreover, ROS accumulation both in chloroplast and cytoplasm were attenuated and defense-related genes were down-regulated in StFC-II overexpression transgenic potato and N. benthamiana leaves. Pi22922 inhibits E3 ubiquitin ligase StCHIP-mediated StFC-II degradation in the cytoplasm and promotes its accumulation in chloroplasts. In summary, this study characterizes a new mechanism that an oomycete RxLR effector suppresses host defenses by promoting StFC-II accumulation in chloroplasts, thereby compromising the host immunity and promoting susceptibility.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141165252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MIKC type MADS-box transcription factor LcSVP2 is involved in dormancy regulation of the terminal buds in evergreen perennial litchi (Litchi chinensis Sonn.) MIKC 型 MADS-box 转录因子 LcSVP2 参与多年生常绿荔枝顶芽的休眠调控

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-05-28 DOI: 10.1093/hr/uhae150

Meng-Meng Ma, Hui-Fen Zhang, Qi Tian, Hui-Cong Wang, Fang-Yi Zhang, Xue Tian, Ren-Fang Zeng, Xu-Ming Huang

{"title":"MIKC type MADS-box transcription factor LcSVP2 is involved in dormancy regulation of the terminal buds in evergreen perennial litchi (Litchi chinensis Sonn.)","authors":"Meng-Meng Ma, Hui-Fen Zhang, Qi Tian, Hui-Cong Wang, Fang-Yi Zhang, Xue Tian, Ren-Fang Zeng, Xu-Ming Huang","doi":"10.1093/hr/uhae150","DOIUrl":"https://doi.org/10.1093/hr/uhae150","url":null,"abstract":"SHORT VEGETATIVE PHASE (SVP), a member of the MADS-box transcription factor family, has been reported to regulate bud dormancy in deciduous perennial plants. Previously, three LcSVPs were identified from litchi genome, and LcSVP2 was highly expressed in the terminal buds of litchi during growth cessation or dormancy stages and down-regulated during growth stages. In this study, the roles of LcSVP2 in governing litchi bud dormancy was examined. LcSVP2 was highly expressed in the shoots, especially in the terminal buds at growth cessation stage, whereas low expression was showed in roots, female flowers and seeds. LcSVP2 was found to be located in the nucleus and have transcription inhibitory activity. Over-expression of LcSVP2 in Arabidopsis thaliana resulted in a later flowering phenotype compared to the wild-type control. Silencing LcSVP2 in growing litchi terminal buds delayed re-entry of dormancy, resulting in significantly lower dormancy rate. The treatment also significantly up-regulated litchi FLOWERING LOCUS T2 (LcFT2). Further study indicates that LcSVP2 interacts with an AP2-type transcription factor, SMALL ORGAN SIZE1 (LcSMOS1). Silencing LcSMOS1 promoted budbreak and delayed bud dormancy. ABA (200 mg/L), which enforced bud dormancy, induced a short-term increase in the expression of LcSVP2 and LcSMOS1. Our study reveals that LcSVP2 may play a crucial role, likely together with LcSMOS1, in dormancy onset of the terminal bud and may also serve as a flowering repressor in evergreen perennial litchi.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141165292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New insights into the evolution and local adaptation of the genus Castanea in East Asia 对东亚蓖麻属植物进化和地方适应性的新认识

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-05-28 DOI: 10.1093/hr/uhae147

Xinghua Nie, Yu Zhang, Shihui Chu, Wenjie Yu, Yang Liu, Boqian Yan, Shuqing Zhao, Wenli Gao, Chaoxin Li, Xueteng Shi, Ruijie Zheng, Kefeng Fang, Ling Qin, Yu Xing

{"title":"New insights into the evolution and local adaptation of the genus Castanea in East Asia","authors":"Xinghua Nie, Yu Zhang, Shihui Chu, Wenjie Yu, Yang Liu, Boqian Yan, Shuqing Zhao, Wenli Gao, Chaoxin Li, Xueteng Shi, Ruijie Zheng, Kefeng Fang, Ling Qin, Yu Xing","doi":"10.1093/hr/uhae147","DOIUrl":"https://doi.org/10.1093/hr/uhae147","url":null,"abstract":"Chestnut plants (Castanea) are important nut fruit trees worldwide. However, little is known regarding the genetic relationship and evolutionary history of different species within the genus. How modern chestnut plants have developed local adaptation to various climates remains a mystery. The genomic data showed that Castanea henryi first diverged in the Oligocene at about 31.56 Million years ago, followed by Castanea mollissima, the divergence between Castanea seguinii and Castanea crenata in the mid-Miocene. Over the last 5 million years, the population of chestnut plants has continued to decline. A combination of selective sweep and environmental association studies was applied to investigate the genomic basis of chestnut adaptation to different climates. 22 candidate genes were associated with temperature and precipitation. We also revealed the molecular mechanism by which CmTOE1 interact with CmZFP8 and CmGIS3 to promote the formation of non-glandular trichomes for adaptation to low temperature and high altitudes. We found a significant expansion of CER1 genes in Chinese chestnut and verified the CmERF48 regulation of CmCER1.6 adaptation to drought environments. These results shed light on the East Asian chestnut plants as a monophyletic group that had completed interspecific differentiation in the Miocene and added candidate gene studies on adaptation to climate change for future studies in nut trees.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141165243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spearmint Targets Microtubules by (−)-Carvone 香薄荷通过(-)-香芹酮作用于微管

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-05-28 DOI: 10.1093/hr/uhae151

Nathalie Hering, Anne-Catherine Schmit, Etienne Herzog, Louis-Thibault Corbin, Leona Schmidt-Speicher, Ralf Ahrens, Marie-Laure Fauconnier, Peter Nick

引用次数: 0

JrPHL8-JrWRKY4-JrSTH2L module regulates resistance to Colletotrichum gloeosporioides in walnut JrPHL8-JrWRKY4-JrSTH2L 模块调控核桃对球孢子菌的抗性

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-05-27 DOI: 10.1093/hr/uhae148

Yutian Mu, Yuhui Dong, Xichen Li, Andi Gong, Haiyi Yu, Changxi Wang, Jianning Liu, Qiang Liang, Ke Qiang Yang, Hongcheng Fang

{"title":"JrPHL8-JrWRKY4-JrSTH2L module regulates resistance to Colletotrichum gloeosporioides in walnut","authors":"Yutian Mu, Yuhui Dong, Xichen Li, Andi Gong, Haiyi Yu, Changxi Wang, Jianning Liu, Qiang Liang, Ke Qiang Yang, Hongcheng Fang","doi":"10.1093/hr/uhae148","DOIUrl":"https://doi.org/10.1093/hr/uhae148","url":null,"abstract":"Walnut anthracnose (Colletotrichum gloeosporioides) reduces walnut yield and quality and seriously threatens the healthy development of the walnut industry. WRKY transcription factors (TFs) are crucial regulatory factors involved in plant-pathogen interactions. Our previous transcriptome analysis results indicate that JrWRKY4 responds to infection by C. gloeosporioides, but its specific regulatory network and disease resistance mechanism are still unclear. Herein, the characteristics of JrWRKY4 as a transcription activator located in the nucleus were first identified. Gain-of-function and loss-of-function analyses showed that JrWRKY4 could enhance walnut resistance against C. gloeosporioides. A series of molecular experiments showed that JrWRKY4 directly interacted with the promoter region of JrSTH2L and positively regulated its expression. In addition, JrWRKY4 interacted with JrVQ4 to form the protein complex, which inhibited JrWRKY4 for the activation of JrSTH2L. Notably, a MYB TF JrPHL8 interacting with the JrWRKY4 promoter was also been identified, which directly bound to the MBS element in the promoter of JrWRKY4 and induced its activity. Our study elucidated a novel mechanism of the JrPHL8-JrWRKY4-JrSTH2L in regulating walnut resistance to anthracnose. This mechanism improves our understanding of the molecular mechanism of WRKY TF mediated resistance to anthracnose in walnut, which provides new insights for molecular breeding of disease-resistant walnuts in the future.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141165186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CRISPR/Cas9 Induced Male-Sterile Line Facilitating Easy Hybrid Production in Polyploid Rapeseed (Brassica napus) CRISPR/Cas9诱导的雄性不育系，便于多倍体油菜（Brassica napus）的杂交生产

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-05-24 DOI: 10.1093/hr/uhae139

Mengxin Tu, Ruisen Wang, Wenhui Guo, Shiqi Xu, Yang Zhu, Jie Dong, Xiangtan Yao, Lixi Jiang

{"title":"A CRISPR/Cas9 Induced Male-Sterile Line Facilitating Easy Hybrid Production in Polyploid Rapeseed (Brassica napus)","authors":"Mengxin Tu, Ruisen Wang, Wenhui Guo, Shiqi Xu, Yang Zhu, Jie Dong, Xiangtan Yao, Lixi Jiang","doi":"10.1093/hr/uhae139","DOIUrl":"https://doi.org/10.1093/hr/uhae139","url":null,"abstract":"Rapeseed is a globally significant oilseed crop cultivated to meet the increasing demand for vegetable oil. In order to enhance yield and sustainability, breeders have adopted the development of rapeseed hybrids as a common strategy. However, current hybrid production systems in rapeseed have various limitations, necessitating the development of a simpler and more efficient approach. In this study, we propose a novel method involving the targeted disruption of Defective in Anther Dehiscence1 of Brassica napus (BnDAD1), an essential gene in the jasmonic acid biosynthesis pathway, using CRISPR/Cas9 technology, to create male-sterile (m-s) lines. BnDAD1 was found to be dominantly expressed in the stamen of rapeseed flower buds. Disrupting BnDAD1 led to decreased levels of α-linolenic acid and jasmonate in the double mutants (DMs), resulting in the defect of anther dehiscence and pollen maturation. By crossing the DM m-s lines with male-fertile lines, a two-line system was demonstrated, enabling the production of F1 seeds. The m-s trait of the bndad1 DM lines was maintainable by applying exogenous methyl jasmonate and subsequently self-pollinating the flowers. This breakthrough holds promising potential for harnessing heterosis in rapeseed and offers a simpler and more efficient method for producing hybrid seeds.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141165371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0