Horticulture Research最新文献_第9页

CaSun1, a SUN family protein, governs the pathogenicity of Colletotrichum camelliae by recruiting CaAtg8 to promote mitophagy CaSun1是一种SUN家族蛋白，通过招募caat8促进线粒体自噬来控制茶树炭素的致病性

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-05-02 DOI: 10.1093/hr/uhaf121

Shuai Meng, Shufen Chao, Meng Xiong, Longjun Cheng, Yu Sun, Li Wang, Ya Chen, Sadhna Jagernath Jane, Chaoxi Luo, Jie Chen

{"title":"CaSun1, a SUN family protein, governs the pathogenicity of Colletotrichum camelliae by recruiting CaAtg8 to promote mitophagy","authors":"Shuai Meng, Shufen Chao, Meng Xiong, Longjun Cheng, Yu Sun, Li Wang, Ya Chen, Sadhna Jagernath Jane, Chaoxi Luo, Jie Chen","doi":"10.1093/hr/uhaf121","DOIUrl":"https://doi.org/10.1093/hr/uhaf121","url":null,"abstract":"Camellia oleifera, a woody oilseed plant native to China, is highly susceptible to anthracnose, a fungal disease that poses a significant threat to its yield and quality. Mitophagy, a specialized form of autophagy that specifically targets dysfunctional mitochondria, is crucial for cellular homeostasis, stress response, and pathogenesis in fungi. The proteins that potentially participate in mitophagy in Colletotrichum camelliae, were identified herein using immunoprecipitation-mass spectrometry (IP-MS) by screening for the potential protein interactors of the core autophagy-related protein, CaAtg8. Among the identified mitochondria-associated proteins, CaSun1 was selected for further investigation. Phenotypic analyses revealed that CaSun1 is a critical regulator of vegetative growth, conidiation, and pathogenicity. CaSun1 localized to the mitochondria, consistent with the conserved function of SUN family proteins. Notably, the findings revealed that CaSun1 was essential for mitophagy and co-localized with CaAtg8 during nitrogen starvation. Functional analyses demonstrated that CaSun1-mediated mitophagy is vital for the growth of invasive hyphae and pathogenicity in C. camelliae. In summary, our findings indicated that CaSun1 mediates mitophagy by facilitating the recruitment of CaAtg8 in C. camelliae, thereby contributing to the establishment of anthracnose. This study provided novel insights into the molecular mechanisms underlying the pathogenesis of fungal infections and identified a potential target for disease control.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"96 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scaffold protein RhCASPL1D1 stabilizes RhPIP2 aquaporins and promotes flower recovery after dehydration in rose (Rosa hybrida) 支架蛋白RhCASPL1D1稳定RhPIP2水通道蛋白，促进玫瑰脱水后花朵恢复

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-05-01 DOI: 10.1093/hr/uhaf119

Kun Liu, Tao Zhang, Siqi Zhao, Jin Chen, Wentong Zhou, Siyu Chen, Yubi Su, Qinglin Liu, Junping Gao, Changqing Zhang

{"title":"Scaffold protein RhCASPL1D1 stabilizes RhPIP2 aquaporins and promotes flower recovery after dehydration in rose (Rosa hybrida)","authors":"Kun Liu, Tao Zhang, Siqi Zhao, Jin Chen, Wentong Zhou, Siyu Chen, Yubi Su, Qinglin Liu, Junping Gao, Changqing Zhang","doi":"10.1093/hr/uhaf119","DOIUrl":"https://doi.org/10.1093/hr/uhaf119","url":null,"abstract":"Water deficit during flowering can lead to petal wilting, necrosis, and sterility, severely limiting crop fertilization and yield. Therefore, rapid recovery of floral organs after dehydration is essential for angiosperms to achieve their full reproductive potential. Aquaporins (AQPs) are bidirectional membrane channels mediating water transmembrane transport. Plasma membrane intrinsic proteins (PIPs), one of AQP subfamily, play a key role in flower opening and dehydration responses. However, it still needs to be elucidated how PIPs are involved in flower recovery after stress. Cut rose (Rosa hybrida), a globally important ornamental flower, undergoes dehydration and rehydration during the postharvest process. Here, we show that the scaffold protein-encoding gene CASP-LIKE PROTEIN 1D1 (RhCASPL1D1), expressed during flower opening and dehydration, promotes flower recovery after dehydration. Silencing RhCASPL1D1 in rose petals and calli hindered cell recovery following dehydration and reduced the rate of water uptake, whereas RhCASPL1D1 overexpression had the opposite effect. Ethylene upregulated RhCASPL1D1 expression, and RhCASPL1D1 physically interacted with RhPIP2s at the plasma membrane. This interaction facilitated RhPIP2s retention to delay its degradation at the plasma membrane and enhanced proteins abundance under dehydration stress. Taken together, our findings reveal a potential mechanism involved in RhCASPL1D1 scaffold regulating flower recovery after dehydration stress.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"11 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and application of genome prediction model in rapeseed: flowering time, yield components and oil content as examples 油菜基因组预测模型的优化与应用——以花期、产量组成和含油量为例

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-05-01 DOI: 10.1093/hr/uhaf115

Wenkai Yu, Xinao Wang, Hui Wang, Wenxiang Wang, Hongtao Cheng, Desheng Mei, Lixi Jiang, Qiong Hu, Jia Liu

{"title":"Optimization and application of genome prediction model in rapeseed: flowering time, yield components and oil content as examples","authors":"Wenkai Yu, Xinao Wang, Hui Wang, Wenxiang Wang, Hongtao Cheng, Desheng Mei, Lixi Jiang, Qiong Hu, Jia Liu","doi":"10.1093/hr/uhaf115","DOIUrl":"https://doi.org/10.1093/hr/uhaf115","url":null,"abstract":"Rapeseed is the second-largest oilseed crop in the world with short domestication and breeding history. This study developed a batch of genomic prediction models for flowering time, oil content and yield components in rapeseed. Using worldwide 404 breeding lines, the optimal prediction model for flowering time, and five quality and yield traits was established by comparison with efficient traditional models and machine learning models. The results indicate that QTLs and significant variations identified by GWAS can significantly improve the prediction accuracy of complex traits, achieving over 90% accuracy in predicting flowering time and thousand grain weight. The GBLUP and Bayes-Lasso models provided the most accurate prediction overall, while machine learning models such as GBDT (Gradient Boosting Decision Trees) exhibited strong predictive performance. Our study provides genome selection solution for the high prediction accuracy and selection of complex traits in rapeseed breeding. The use of a diverse panel of 404 worldwide lines ensures that the findings are broadly applicable across different rapeseed breeding programs.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"46 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the molecular secrets of Paeonia plants: advances in key gene mining and molecular breeding technology 揭开芍药植物的分子秘密：关键基因挖掘和分子育种技术进展

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-05-01 DOI: 10.1093/hr/uhaf090

Daqiu Zhao, Honglei An, Jun Tao

{"title":"Unlocking the molecular secrets of Paeonia plants: advances in key gene mining and molecular breeding technology","authors":"Daqiu Zhao, Honglei An, Jun Tao","doi":"10.1093/hr/uhaf090","DOIUrl":"https://doi.org/10.1093/hr/uhaf090","url":null,"abstract":"Paeonia plants are famous for their ornamental, medicinal and oil values. Due to the popularity of seed oil and cut flowers in the market, the mechanisms underlying related traits of Paeonia plants have been fascinating, and the research work on them has increased rapidly in recent years, urging a comprehensive review of their research progress. To unlock the molecular secrets of Paeonia plants, we first summarize the latest advances in their genome research. More importantly, we emphasize the key genes involved in plant growth and development processes, such as bud dormancy, flowering regulation, seed oil formation, flower coloration, stem strength regulation, fragrance emission, as well as plant resistance to stress including drought, high-temperature, low-temperature, salt and waterlogging stresses and biotic stress. In addition, the advances in molecular breeding technology of Paeonia plants are highlighted, such as molecular marker, genetic map, localization of QTLs, tissue culture and genetic transformation system. This review covers advances in the past decades and provides valuable insights into the perspectives for the key gene mining and molecular breeding technology of Paeonia plants, which would breed new Paeonia varieties through molecular breeding technology.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"18 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic dynamics and temporal heritability of tomato architectural traits using an unmanned ground vehicle-based plant phenotyping system 基于无人地面车辆植物表型系统的番茄建筑性状的表型动力学和时间遗传力

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-05-01 DOI: 10.1093/hr/uhaf109

Pengyao Xie, Xin Yang, Leisen Fang, Tonglin Wang, Jirong Zheng, Yu Jiang, Haiyan Cen

{"title":"Phenotypic dynamics and temporal heritability of tomato architectural traits using an unmanned ground vehicle-based plant phenotyping system","authors":"Pengyao Xie, Xin Yang, Leisen Fang, Tonglin Wang, Jirong Zheng, Yu Jiang, Haiyan Cen","doi":"10.1093/hr/uhaf109","DOIUrl":"https://doi.org/10.1093/hr/uhaf109","url":null,"abstract":"Large-scale manual measurements of plant architectural traits in tomato growth are laborious and subjective, hindering deeper understanding of temporal variations in gene expression heterogeneity. This study develops a high-throughput approach for characterizing tomato architectural traits at different growth stages and mapping temporal broad-sense heritability using an unmanned ground vehicle-based plant phenotyping system. The SegFormer with fusion of multispectral and depth imaging modalities was employed to semantically segment plant organs from the registered RGB-D and multispectral images. Organ point clouds were then generated and clustered into instances. Finally, six key architectural traits, including fruit spacing (FS), inflorescence height (IH), stem thickness (ST), leaf spacing (LS), total leaf area (TLA), and leaf inclination angle (LIA) were extracted and the temporal broad-sense heritability folds were plotted. The RMSEs of the estimated FS, IH, ST and LS were 0.014 m, 0.043 m, 0.003 m and 0.015 m. The visualizations of the estimated TLA and LIA matched the actual growth trends. The broad-sense heritability of the extracted traits exhibited different trends across the growth stages: i) ST, IH, and FS had a gradually increased broad sense heritability over time, ii) LS and LIA had a decreasing trend, and iii) TLA showed fluctuations (i.e., an M-shaped pattern) of the broad sense heritability throughout the growth period. The developed system and analytical approach are promising tools for accurate and rapid characterization of spatiotemporal changes of tomato plant architecture in controlled environments, laying the foundation for efficient crop breeding and precision production management in the future.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"34 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two DNA-binding One Zinc Finger (DOF) transcription factors MdCDOF3 and MdDOF3.6 accelerate leaf senescence by activating cytokinin oxidase MdCKX7 in response to sorbitol signaling in apple 两个dna结合的One Zinc Finger （DOF）转录因子mddof3和MdDOF3.6响应山梨糖醇信号，通过激活细胞分裂素氧化酶MdCKX7加速叶片衰老

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-04-29 DOI: 10.1093/hr/uhaf120

Wang-Jiang Zhang, Chang-Ning Ma, Lian-Da Du, Ying Xiang, Fan Xiao, Ya-Ting Liu, Chu-Kun Wang, Wan-Kun Li, Ting-Ting Zhao, Da-Gang Hu

{"title":"Two DNA-binding One Zinc Finger (DOF) transcription factors MdCDOF3 and MdDOF3.6 accelerate leaf senescence by activating cytokinin oxidase MdCKX7 in response to sorbitol signaling in apple","authors":"Wang-Jiang Zhang, Chang-Ning Ma, Lian-Da Du, Ying Xiang, Fan Xiao, Ya-Ting Liu, Chu-Kun Wang, Wan-Kun Li, Ting-Ting Zhao, Da-Gang Hu","doi":"10.1093/hr/uhaf120","DOIUrl":"https://doi.org/10.1093/hr/uhaf120","url":null,"abstract":"Leaf senescence, an essential component of the plant life cycle, seriously affects the productivity of numerous commercial crops, with cytokinins serving as crucial regulators in delaying this process. Here, we observed that apple (Malus domestica) leaves exhibiting deficiencies in sorbitol synthesis due to antisense inhibition of ALOSE-6PHOSPHATE REDUCTASE (A6PR) presented an increase in cytokinin content and exhibited a delay in leaf senescence, in contrast to wild-type (WT) leaves. Transcriptome analysis indicated that the expression of cytokinin oxidase 7 (MdCKX7), encoding a key enzyme in the cytokinin degradation pathway, was significantly downregulated in the A6PR antisense lines. Functional verification confirmed that MdCKX7 facilitated the degradation of cytokinin and accelerated leaf senescence. Moreover, this leaf senescence phenotype was exacerbated by the co-expression of two DNA-binding One Zinc Finger (DOF) transcription factors, cycling DOF factor 3 (MdCDOF3) and MdDOF3.6, along with MdCKX7. Further biochemical and phenotypic analyses demonstrated that MdCDOF3 and MdDOF3.6 bind directly to the promoter region of MdCKX7, thereby transcriptionally activating its expression. Intriguingly, the expression of MdCDOF3, MdDOF3.6 and MdCKX7 is cooperatively induced by sorbitol. These findings demonstrate that the MdCDOF3/MdDOF3.6-MdCKX7 regulatory module orchestrates leaf senescence by facilitating cytokinin degradation in response to sorbitol signaling, revealing a mechanism by which sorbitol signaling modulates leaf senescence specifically through MdCKX7-mediated cytokinin degradation in apple plants.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"35 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies on the mother flower carnation: past, present and future 母花康乃馨的研究：过去、现在和未来

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-04-29 DOI: 10.1093/hr/uhaf118

Min Wang, Zhengkang Pi, Zekang Pan, Xilin Li, Linlin Zhong, Yunjiang Cheng, Manzhu Bao, Fan Zhang

引用次数: 0

High-quality Lindera megaphylla genome analysis provides insights into genome evolution and allows for the exploration of genes involved in terpenoid biosynthesis 高质量的巨葡萄树基因组分析提供了基因组进化的见解，并允许探索涉及萜类生物合成的基因

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-04-27 DOI: 10.1093/hr/uhaf116

Hongli Liu, Jing Liu, Yun Bai, Xinran Zhang, Qingzheng Jiao, Peng Chen, Ruimin Li, Yan Li, Wenbin Xu, Yanhong Fu, Jiuxing Lu, Xiaoming Song, Yonghua Li

{"title":"High-quality Lindera megaphylla genome analysis provides insights into genome evolution and allows for the exploration of genes involved in terpenoid biosynthesis","authors":"Hongli Liu, Jing Liu, Yun Bai, Xinran Zhang, Qingzheng Jiao, Peng Chen, Ruimin Li, Yan Li, Wenbin Xu, Yanhong Fu, Jiuxing Lu, Xiaoming Song, Yonghua Li","doi":"10.1093/hr/uhaf116","DOIUrl":"https://doi.org/10.1093/hr/uhaf116","url":null,"abstract":"Lindera megaphylla, a Lauraceae species, is valued for timber, horticulture, and traditional medicine. Here, a high-quality genome of L. megaphylla was obtained at the chromosome level. A total of 96.77% of genomic sequences were mapped onto 12 chromosomes, with a total length of 1,309.2 megabase (Mb) and an N50 scaffold of 107.75 Mb. Approximately 75.91% of genome consists of repetitive sequences, and 7,004 ncRNAs were predicted. We identified 29,482 genes, and 28,657 genes were annotated. Gene family analysis showed expanded gene families were mainly involved in energy metabolism and cellular growth, while contracted ones were associated with carbohydrate metabolism and signal transduction. Our analysis revealed that L. megaphylla has undergone two rounds of whole-genome duplication (WGD). Our results revealed that volatile compounds in L. megaphylla leaves inhibited the growth of several fungi and bacteria. Fifty-two terpene synthase (TPS) genes were identified and classified into six subfamilies, with significant expansion observed in the TPS-b, TPS-f, and TPS-g subfamilies in L. megaphylla. Transcriptomic and metabolomic co-analysis revealed that 43 DEGs were correlated with 117 terpenoids. Further analysis revealed that LmTPS1 was significantly correlated with caryophyllene oxide content. The overexpression of LmTPS1 in transgenic tomato lines significantly increased the contents of β-Caryophyllene and humulene, which further improved the resistance of transgenic tomato plants to common fungal and bacterial diseases. The integrated analysis of genome, metabolome, and transcriptome provides comprehensive insights into the evolution of L. megaphylla and clarifies the molecular mechanisms underlying the protective effects of caryophyllene against biotic stress.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"36 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linking the structure of vascular bundles and mineral element deposition reveals the hub role of nodes in bamboo 将维管束结构与矿物元素沉积联系起来，揭示了竹节的枢纽作用

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-04-24 DOI: 10.1093/hr/uhaf113

Xianyu Pan, Ji Feng Shao

{"title":"Linking the structure of vascular bundles and mineral element deposition reveals the hub role of nodes in bamboo","authors":"Xianyu Pan, Ji Feng Shao","doi":"10.1093/hr/uhaf113","DOIUrl":"https://doi.org/10.1093/hr/uhaf113","url":null,"abstract":"Nodes are a distinct feature of bamboo plants, categorized into three main types: culm, shoot, and rhizome nodes. However, the latter two are often overlooked due to their underground growth, resulting in a limited understanding of their structure and function. In this study, we examined the structure and mineral elements deposition in the nodes of Moso bamboo (Phyllostachys edulis). Our findings indicate that all three node types possess a complex yet well-organized vascular bundle system, with notable differences. Culm nodes feature enlarged vascular bundles with distinct xylem and phloem regions, whereas shoot and rhizome nodes have less-developed phloem regions. The rhizome node contains a vascular structure of crown root and coronary shoot bud, which is absent in culm and shoot nodes. In culm node, iron accumulation decreases gradually from the bottom to the top, primarily localizing in cells near the enlarged and small vascular bundles. Zinc is deposited in both the enlarged and small vascular bundles in the lower part of the node. In contrast, calcium accumulates predominantly in the upper part, particularly in cells adjacent to enlarged and small vascular bundles including diffuse and parenchyma cells. Potassium is distributed throughout most cells but is less abundant in the pith cavity and xylem transfer cells. In shoot and rhizome nodes, iron, zinc, calcium, and potassium exhibit specific regional and cellular deposition patterns. Overall, the vascular structure and mineral element deposition patterns suggest that bamboo nodes function not only as tissue junctions but also as critical hubs for mineral element deposition and distribution.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"7 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AcABI5a integrates abscisic acid signaling to developmentally modulate fruit ascorbic acid biosynthesis in kiwifruit AcABI5a整合脱落酸信号，调控猕猴桃果实抗坏血酸的生物合成

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-04-24 DOI: 10.1093/hr/uhaf111

Xiaoying Liu, Yachen Li, Xianzhi Zhang, Xiaodong Xie, Abu Naim Md Muzahid, Jing Tu, Lansha Luo, Gudeta Chalchisa, Haiyan Lv, Hua Tian, Sean M Bulley, Dawei Li, Caihong Zhong

{"title":"AcABI5a integrates abscisic acid signaling to developmentally modulate fruit ascorbic acid biosynthesis in kiwifruit","authors":"Xiaoying Liu, Yachen Li, Xianzhi Zhang, Xiaodong Xie, Abu Naim Md Muzahid, Jing Tu, Lansha Luo, Gudeta Chalchisa, Haiyan Lv, Hua Tian, Sean M Bulley, Dawei Li, Caihong Zhong","doi":"10.1093/hr/uhaf111","DOIUrl":"https://doi.org/10.1093/hr/uhaf111","url":null,"abstract":"Consumers value highly the nutritional content and flavor of fresh fruits, which are influenced by endogenous plant hormones. However, the molecular mechanisms governing the hormonal regulation of essential nutrients such as ascorbic acid (AsA) in fruit are still unclear. This study investigates the regulation of AsA synthesis in kiwifruit by the transcription factor AcABI5a, which is involved in mediating the abscisic acid (ABA) signal. A negative correlation between AcABI5a expression and AsA levels across different developmental stages of kiwifruit was observed. Furthermore, AcABI5a was found to bind both the AcMYBS1 promoter, repressing its transcriptional activity, and its own promoter, fostering expression and maintaining active repression of AcMYBS1. AcMYBS1 activates the expression of AcGGP3, which encodes an enzymatic step in AsA biosynthesis that is highly regulated both transcriptionally and translationally. In-depth interaction studies utilizing yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC), firefly luciferase complementation (NC-LUC), and pull-down assays unveiled that AcABI5a also physically interacts with AcMYBS1, further impeding its activation of AcGGP3. Results from knock-out by gene editing and overexpression of AcABI5a support the role of AcABI5a in mediating the ABA inhibitory effect on AsA synthesis by repressing the expression of AcMYBS1 and thus AcGGP3. Overall, our findings highlight AcABI5a’s negative regulatory role in AsA synthesis by integrating ABA signaling during fruit development, providing new insights into the regulation of AsA synthesis by phytohormones.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"2 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0