Horticulture Research最新文献_第9页

The AP2 transcription factor CsAIL6 negatively regulates citric acid accumulation in citrus fruits by interacting with a WD40 protein CsAN11 AP2转录因子CsAIL6通过与WD40蛋白CsAN11相互作用负向调控柑橘果实中柠檬酸的积累

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-06 DOI: 10.1093/hr/uhaf002

Han Han, Yu-Jia Li, Shariq Mahmood Alam, Tian Zhou, Muhammad Abbas Khan, Aye Myat Thu, Yong-Zhong Liu

{"title":"The AP2 transcription factor CsAIL6 negatively regulates citric acid accumulation in citrus fruits by interacting with a WD40 protein CsAN11","authors":"Han Han, Yu-Jia Li, Shariq Mahmood Alam, Tian Zhou, Muhammad Abbas Khan, Aye Myat Thu, Yong-Zhong Liu","doi":"10.1093/hr/uhaf002","DOIUrl":"https://doi.org/10.1093/hr/uhaf002","url":null,"abstract":"Citric acid accumulation is an essential process in citrus fruits that determines fruit flavor and marketability. The MBW complex transcription factor genes, CsAN11, CsAN1, and CsPH4 play key roles in regulating citric acid accumulation. Although how to regulate CsAN1 or CsPH4 was widely investigated, studies on the regulation of CsAN11 are scarce. In this study, we characterized the AP2/ERF (APETALA2/ethylene response factor) transcription factor gene CsAIL6, which is lowly expressed in high-acid citrus varieties and highly expressed in low-acid citrus varieties. Overexpressing CsAIL6 obviously decreased the citric acid content in citrus fruits, calli or tomatoes, whereas silencing CsAIL6 significantly increased the fruit citric acid content. Additionally, transcript levels of CsAN11, CsAN1, and CsPH4 were significantly increased by silencing CsAIL6; only the CsAN11 transcript level was significantly decreased by overexpressing CsAIL6. Similarly, only the tomato AN11 (SIAN11) transcript level in CsAIL6 stably overexpressing fruits was markedly lower than that in WT fruits. Further experiments revealed that overexpressing CsAN11 significantly increased the organic acid content but had no obvious influence on the CsAIL6 transcript level; in addition, CsAIL6 only interacts with CsAN11, rather than with CsAN1 and CsPH4 of the MBW complex. Taken together, our findings verified that CsAIL6 negatively regulates citric acid accumulation through directly interacting with the WD40 protein CsAN11, which provides a new mechanism for citric acid accumulation in fruits through the regulation of the MBW complex.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"5 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142935116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SmCSN5 is a synergist in the transcription factor SmMYB36 mediated tanshinones and phenolic acids biosynthesis in Salvia miltiorrhiza SmCSN5是转录因子SmMYB36介导丹参酮和酚酸生物合成的增效剂

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-06 DOI: 10.1093/hr/uhaf005

Qi Li, Xiujuan Wang, Jie Wang, Yan Su, Yuanyi Guo, Jie Yang, Jingying Liu, Zheyong Xue, Juane Dong, Pengda Ma

{"title":"SmCSN5 is a synergist in the transcription factor SmMYB36 mediated tanshinones and phenolic acids biosynthesis in Salvia miltiorrhiza","authors":"Qi Li, Xiujuan Wang, Jie Wang, Yan Su, Yuanyi Guo, Jie Yang, Jingying Liu, Zheyong Xue, Juane Dong, Pengda Ma","doi":"10.1093/hr/uhaf005","DOIUrl":"https://doi.org/10.1093/hr/uhaf005","url":null,"abstract":"The ubiquitin-26S proteasome system (UPS) is associated with protein stability and activity, regulation of hormone signaling, and the production of secondary metabolites in plants. Though the mechanism of action of SmMYB36 on the tanshinone and phenolic acid biosynthesis is well understood, its regulation through post-translational modifications is unclear. A constitutive photomorphogenesis 9 (COP9) signalosome subunit 5 (SmCSN5), which interacted with SmMYB36 and inhibited its ubiquitination-based degradation, was identified in S. miltiorrhiza. SmCSN5 promoted tanshinone biosynthesis but inhibited phenolic acid biosynthesis in the hairy roots of S. miltiorrhiza. SmMYB36 also activated the transcription of the target genes: SmDXS2 and SmCPS1 but repressed that of SmRAS in a SmCSN5-dependent manner. SmCSN5 acts as a positive regulator in MeJA-induced biosynthesis of tanshinones and phenolic acids. Specifically, SmCSN5 alone, when expressed transiently in tobacco and rice protoplasts, was localized to the cytoplasm, cell membrane, and nucleus, whereas when coexpressed with SmMYB36, it was detected only in the nucleus. Additionally, the degradation of SmMYB361-153 by ubiquitination was lowered after truncation of the self-activating structural domain of SmMYB36154-160. Collectively, these results suggest that SmCSN5 affected the transcriptional activation of SmMYB36 and stabilized SmMYB36, providing insights into the SmMYB36-based regulation of the accumulation of tanshinone and phenolic acid at the transcriptional and post-translational levels.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"48 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142935011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BrWRKY8: A Key Regulatory Factor Involved in Delaying Postharvest Leaf Senescence of Pakchoi (Brassica rapa subsp. chinensis) by 2,4-Epibrassinolide BrWRKY8：延缓小白菜采后叶片衰老的关键调控因子2,4-表油菜素内酯

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-06 DOI: 10.1093/hr/uhaf004

Xuesong Liu, Yinghao Xu, Yujun Zhang, Xiaofei Chen, Pengxia Li

{"title":"BrWRKY8: A Key Regulatory Factor Involved in Delaying Postharvest Leaf Senescence of Pakchoi (Brassica rapa subsp. chinensis) by 2,4-Epibrassinolide","authors":"Xuesong Liu, Yinghao Xu, Yujun Zhang, Xiaofei Chen, Pengxia Li","doi":"10.1093/hr/uhaf004","DOIUrl":"https://doi.org/10.1093/hr/uhaf004","url":null,"abstract":"Brassinosteroids (BRs) are extensively distributed in plants and play crucial roles throughout all stages of plant growth. Nevertheless, the molecular mechanism through which BRs influence postharvest senescence in pakchoi remains elusive. Previous studies have demonstrated that application of 1.5 μM of the BRs analog 2,4-epibrassinolide (EBR) delayed the leaf senescence in harvested pakchoi. In this study, we constructed the EBR-delayed senescence transcriptome in pakchoi leaves and discovered that EBR modulates the expression of genes involved in the chlorophyll (Chl) metabolism pathway and the BRs pathway in pakchoi. Notably, we identified and characterized an EBR-suppressed, nucleus-localized WRKY transcription factor (TF) called BrWRKY8. BrWRKY8 is a highly expressed transcriptional activator in senescent leaves, targeting the promoters of the Chl degradation associated gene BrSGR2 and the BRs degradation associated gene BrCHI2, thereby promoting their expression. Overexpression of the BrWRKY8 gene accelerated the senescence process in Arabidopsis leaves, while EBR treatment mitigated the leaf senescence phenotype induced by BrWRKY8 overexpression. Conversely, silencing of BrWRKY8 through the virus-induced gene silencing (VIGS) extended the postharvest storage period of pakchoi. In conclusion, the newly discovered BRs-BrWRKY8 regulatory model in this study provides novel insights into BRs-mediated leaf senescence in pakchoi.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"20 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142935012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of storage organ formation by long-distance tuberigen signals in potato 马铃薯长距离结核菌素信号对贮藏器官形成的调控

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-03 DOI: 10.1093/hr/uhae360

Xinru Bao, Yunke Zhu, Guangcun Li, Lu Liu

引用次数: 0

VaWRKY65 contributes to cold tolerance through dual regulation of soluble sugar accumulation and reactive oxygen species scavenging in Vitis amurensis vwrky65基因通过双重调控葡萄可溶性糖积累和活性氧清除来促进葡萄的耐寒性

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-03 DOI: 10.1093/hr/uhae367

Lin Meng, Huimin Zhou, Lisha Tan, Qingyun Li, Yujun Hou, Wenjuan Li, Subash Kafle, Ju Liang, Rishi Aryal, Zhenchang Liang, Haiping Xin

{"title":"VaWRKY65 contributes to cold tolerance through dual regulation of soluble sugar accumulation and reactive oxygen species scavenging in Vitis amurensis","authors":"Lin Meng, Huimin Zhou, Lisha Tan, Qingyun Li, Yujun Hou, Wenjuan Li, Subash Kafle, Ju Liang, Rishi Aryal, Zhenchang Liang, Haiping Xin","doi":"10.1093/hr/uhae367","DOIUrl":"https://doi.org/10.1093/hr/uhae367","url":null,"abstract":"Although the significance of some plant WRKYs in response to cold stress have been identified, the molecular mechanisms of most WRKYs remain unclear in grapevine. In this study, we demonstrate that cold-induced expression of VaBAM3 in Vitis amurensis executes a beneficial role in enhancing resistance by the regulating starch decomposition. VaWRKY65 was identified as an upstream transcriptional activator of VaBAM3 through yeast one-hybrid library screening and validated to directly interacts with the W-box region inside the VaBAM3 promoter. Transgenic Arabidopsis thaliana plants and grapevine roots overexpression VaWRKY65 exhibited improved cold tolerance along with higher BAM activity and soluble sugar levels, whereas opposite changes were observed in VaWRKY65 knockdown lines created by virus-induced gene silencing (VIGS) in grapevine plants and in the knockout wrky65 mutants generated by CRISPR/Cas9 technology in grapevine roots. The transcriptome data show that overexpression of VaWRKY65 led to significant alteration of a diverse set of stress-related genes at the transcriptional level. One of the genes, Peroxidase 36 (VaPOD36) was further verified as a direct target of VaWRKY65. Consistently, VaWRKY65-overexpressing plants had higher VaPOD36 transcript levels and POD activity but a reduced ROS level, while silencing VaWRKY65 results in contrary changes. Collectively, these results reveal that VaWRKY65 enhanced cold tolerance through modulating soluble sugars produced from starch breakdown and ROS scavenging.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"1 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide association study of salicylic acid provides genetic insights for tea plant selective breeding 水杨酸的全基因组关联研究为茶树选择性育种提供了遗传学启示

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-02 DOI: 10.1093/hr/uhae362

Xiuling Deng, Dejun Huang, Yihan Wang, Hongwei An, Dingchen Bai, Xiaojing Wang, Suzhen Niu, Xiaoming Song

{"title":"Genome-wide association study of salicylic acid provides genetic insights for tea plant selective breeding","authors":"Xiuling Deng, Dejun Huang, Yihan Wang, Hongwei An, Dingchen Bai, Xiaojing Wang, Suzhen Niu, Xiaoming Song","doi":"10.1093/hr/uhae362","DOIUrl":"https://doi.org/10.1093/hr/uhae362","url":null,"abstract":"Salicylic acid (SA) is a phenolic phytohormone widely believed to regulate plant growth and stress response. Despite its significance, the genetic basis of SA-mediated resistance to biotic stressors in tea plants is little understood. Our study investigated the genetic diversity, population structure, and linkage disequilibrium (LD) patterns of 299 tea accessions using 79 560 high-quality single nucleotide polymorphisms (SNPs) obtained from genotyping-by-sequencing (GBS) data. Our genome-wide association study identified CSS0033791.1, an essential gene encoding 9-cis-epoxycarotenoid dioxygenase (CsNCED1), which catalyzes a vital step in abscisic acid (ABA) biosynthesis. Exogenous ABA treatment and transgenic overexpression of the CsNCED1 gene lowered SA content in the respective tea plants by inhibiting the expression of the ICS gene. Further analysis revealed that ABA could reduce the expression levels of the SA receptor gene (NPR1) and NPR1 target genes (PR1 and WRKY18), increasing the plant’s susceptibility to biotic stressors. Furthermore, the feeding behavior of Spodoptera litura (S. litura) revealed that the insect bite area on transgenic leaves was substantially more extensive than that in wild-type, implying that the CsNCED1 gene had a negative regulatory role in SA-mediated immune response. This study thus provides the foundation for future insect resistance breeding, sustainable tea plant resource usage, and molecular marker-assisted (MAS) tea plant breeding.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"27 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142917182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flavonoids as key players in cold tolerance: molecular insights and applications in horticultural crops 黄酮类化合物在园艺作物抗寒性中的关键作用：分子认识及其应用

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-02 DOI: 10.1093/hr/uhae366

Jiaxin Li, Qinhan Yu, Chang Liu, Ningbo Zhang, Weirong Xu

{"title":"Flavonoids as key players in cold tolerance: molecular insights and applications in horticultural crops","authors":"Jiaxin Li, Qinhan Yu, Chang Liu, Ningbo Zhang, Weirong Xu","doi":"10.1093/hr/uhae366","DOIUrl":"https://doi.org/10.1093/hr/uhae366","url":null,"abstract":"Cold stress profoundly affects the growth, development, and productivity of horticultural crops. Among the diverse strategies plants employ to mitigate the adverse effects of cold stress, flavonoids have emerged as pivotal components in enhancing plant resilience. This review was written to systematically highlight the critical role of flavonoids in plant cold tolerance, aiming to address the increasing need for sustainable horticultural practices under climate stress. We provide a comprehensive overview of the role of flavonoids in the cold tolerance of horticultural crops, emphasizing their biosynthesis pathways, molecular mechanisms, and regulatory aspects under cold stress conditions. We discuss how flavonoids act as antioxidants, scavenging reactive oxygen species (ROS) generated during cold stress, and how they regulate gene expression by modulating stress-responsive genes and pathways. Additionally, we explore the application of flavonoids in enhancing cold tolerance through genetic engineering and breeding strategies, offering insights into practical interventions for improving crop resilience. Despite significant advances, a research gap remains in understanding the precise molecular mechanisms by which specific flavonoids confer cold resistance, especially across different crop species. By addressing current knowledge gaps, proposing future research directions, and highlighting implications for sustainable horticulture, we aim to advance strategies to enhance cold tolerance in horticultural crops.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"41 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142917332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A single-cell transcriptomic atlas reveals the cell differentiation trajectory and the response to virus invasion in swelling clove of garlic 单细胞转录组图谱揭示了大蒜肿瓣细胞分化轨迹和对病毒入侵的反应

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-31 DOI: 10.1093/hr/uhae365

Song Gao, Fu Li, Zheng Zeng, Qiaoyun He, Hassan H A Mostafa, Suling Zhang, Taotao Wang, Yanzhou Wang, Touming Liu

{"title":"A single-cell transcriptomic atlas reveals the cell differentiation trajectory and the response to virus invasion in swelling clove of garlic","authors":"Song Gao, Fu Li, Zheng Zeng, Qiaoyun He, Hassan H A Mostafa, Suling Zhang, Taotao Wang, Yanzhou Wang, Touming Liu","doi":"10.1093/hr/uhae365","DOIUrl":"https://doi.org/10.1093/hr/uhae365","url":null,"abstract":"The garlic bulb comprises several cloves, the swelling growth of which is significantly hindered by the accumulation of viruses. Herein, we describe a single-cell transcriptomic atlas of swelling cloves with virus accumulation, which comprised 19 681 high-quality cells representing 11 distinct cell clusters. Cells of two clusters, clusters 7 (C7) and 11 (C11), were inferred to be from the meristem. Cell trajectory analysis suggested the differentiation of clove cells to start from the meristem cells, along two pseudo-time paths. Investigation into the cell-specific activity of invasive viruses demonstrated that garlic virus genes showed relatively low expression activity in cells of the clove meristem. There were 2060 garlic genes co-expressed with virus genes, many of which showed an association with the defense response. Five glutathione synthase/reductase genes co-expressed with virus genes displayed up-regulated expression, and the glutathione and related metabolites level showed an alteration in virus-invasive garlic clove, implying the role of glutathione in viral immunity of garlic. Our study offers valuable insights into the clove organogenesis and interaction between garlic and virus at single-cell resolution.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"82 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142917752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The long non-coding RNA MSTRG.32189-PcmiR399b-PcUBC24 module regulates the phosphate accumulation and disease resistance to Botryosphaeria dothidea in pear 长链非编码RNA MSTRG.32189-PcmiR399b-PcUBC24模块调控梨中磷的积累和对马铃薯芽孢杆菌的抗性

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-30 DOI: 10.1093/hr/uhae359

Yuekun Yang, Shamei Lv, Xiaosan Huang, Ying He, Xiaoyan Zhang, Yu Liu, Guoping Wang, Ni Hong, Liping Wang

{"title":"The long non-coding RNA MSTRG.32189-PcmiR399b-PcUBC24 module regulates the phosphate accumulation and disease resistance to Botryosphaeria dothidea in pear","authors":"Yuekun Yang, Shamei Lv, Xiaosan Huang, Ying He, Xiaoyan Zhang, Yu Liu, Guoping Wang, Ni Hong, Liping Wang","doi":"10.1093/hr/uhae359","DOIUrl":"https://doi.org/10.1093/hr/uhae359","url":null,"abstract":"Pear ring rot disease (Botryosphaeria dothidea) causes a significant threat to the healthy development of the pear industry. Recent research has identified the functional role of long noncoding RNAs (lncRNAs) in various biological processes of plants. The role of lncRNAs in pear defense response remains unknown. In this study, transcriptome sequencing was used to analyze long non-coding RNAs in pear stem infected with B. dothidea. It showed that 3,555 lncRNAs were identified, of which 286 lncRNAs were significantly differentially expressed. GO and KEGG analysis showed that cis- and trans-regulated target genes were enriched in multiple disease-resistant related pathways. More specifically, MSTRG.32189 predicted as an endogenous target mimic (eTM) was significantly down-regulated in response to B. dothidea infection, which was confirmed to inhibit the cleavage effect of PcmiR399b on PcUBC24. The transgenic Arabidopsis of OE-MSTRG.32189 exhibited lower Pi content and weaker disease resistance to Botrytis cinerea compared to WT. In pear callus, overexpression of MSTRG.32189 negatively regulated PcmiR399b, which decreased Pi content and reduced disease resistance. Whereas overexpressing PcmiR399b in pear callus exhibited the opposite effects compared with OE-MSTRG.32189. Overexpression and knock-out of PcUBC24 further clarified that PcUBC24 negatively regulate the Pi content and disease resistance to B. dothidea infection. Furthermore, the ROS levels and the expressions of disease resistance pathway-related genes were regulated by the MSTRG.32189-PcmiR399b-PcUBC24 module in transgenic pear callus, which contributed to disease resistance. Overall, our results demonstrated the role of lncRNAs in pear defense response, revealing MSTRG.32189-PcmiR399b-PcUBC24 module regulates phosphate accumulation and disease resistance to B. dothidea infection in pear.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"25 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142917331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of nitrate use efficiency in tea plant (Camellia sinensis) based on leaf chlorate sensitivity 基于叶片氯酸盐敏感性的茶树硝酸盐利用效率表征

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-28 DOI: 10.1093/hr/uhae354

Wenjing Zhang, Xiaoying Dong, Kang Ni, Lifeng Ma, Lizhi Long, Jianyun Ruan

{"title":"Characterization of nitrate use efficiency in tea plant (Camellia sinensis) based on leaf chlorate sensitivity","authors":"Wenjing Zhang, Xiaoying Dong, Kang Ni, Lifeng Ma, Lizhi Long, Jianyun Ruan","doi":"10.1093/hr/uhae354","DOIUrl":"https://doi.org/10.1093/hr/uhae354","url":null,"abstract":"Nitrate (NO3−), a key form of inorganic nitrogen (N) in soils, is typically lost in tea gardens through leaching. However, NO3− utilization efficiency (NiUE) and its characteristic mechanism in tea plants remain unclear. This study screened contrastive genotypes of NiUE using leaf chlorate sensitivity and explored the potential genes that regulate this process. Fresh branches of ten cultivars were hydroponically cultivated and subjected to potassium nitrate (KNO3) and potassium chlorate (KClO3) treatments, with the former as the control group. The sensitive cultivar, Zhenong 117 (ZN117), showed a decrease in SPAD and Fv/Fm values following KClO3 treatment, while the tolerant cultivar, Teiguanyin (TGY), exhibited minimal significant changes. After 5 days of cultivation, the 15N concentration and proportion in new shoots of ZN117 were significantly higher than those in TGY. Transcriptome analysis revealed that the expression of genes responsible for NO3− transport, including the nitrate transporters NRT2.4, NPF4.6, NPF6.1, NPF1.10, and NPF1.11, significantly increased in ZN117 after NO3− supply. Genes involved in NO3− reduction, chlorophyll synthesis, and photosynthesis were progressively induced. Co-expression network analysis indicated that the squamosa promoter binding protein activated the onset of NO3− signaling, while basic helix–loop–helix transcripts were triggered to higher levels during NO3− supply. This study proposes a rapid characterization method of NiUE in woody plants and a speculative molecular regulatory mechanism for the NO3− transfer and remobilization of tea plants. A set of specific genes involved in NO3− transport, reduction, and mobilization were identified and proposed as marker genes for NiUE in tea plants.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"26 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142887991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0