Horticulture Research最新文献_第9页

Accumulation of dually-targeted StGPT1 in chloroplasts mediated by StRFP1, an E3 ubiquitin ligase, enhances plant immunity 由 E3 泛素连接酶 StRFP1 介导的叶绿体中双靶标 StGPT1 的积累可增强植物免疫力

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-08-28 DOI: 10.1093/hr/uhae241

Xintong Wu, Xiaoshuang Zhou, Tianyu Lin, Zhe Zhang, Xinya Wu, Yonglin Zhang, Yanli Liu, Zhendong Tian

{"title":"Accumulation of dually-targeted StGPT1 in chloroplasts mediated by StRFP1, an E3 ubiquitin ligase, enhances plant immunity","authors":"Xintong Wu, Xiaoshuang Zhou, Tianyu Lin, Zhe Zhang, Xinya Wu, Yonglin Zhang, Yanli Liu, Zhendong Tian","doi":"10.1093/hr/uhae241","DOIUrl":"https://doi.org/10.1093/hr/uhae241","url":null,"abstract":"Chloroplasts play a crucial role in essential processes such as photosynthesis and the synthesis of primary and diverse secondary metabolites. Recent studies have also highlighted their significance linked to phytohormone production in plant immunity, especially SA and JA. Ubiquitination, a key post-translational modification, usually leads to target protein degradation, which acts as a signal for remodeling the proteome via the induction of protein endocytosis or targeting to other membrane associated systems. Previously, the potato E3 ligase StRFP1 was shown to enhance resistance against Phytophthora infestans, but its mechanism remained unclear. Here, we demonstrate that StRFP1 interacted with the dually localized plastid glucose 6-phosphate transporter StGPT1 on the endoplasmic reticulum (ER). Transient expressed StGPT1-GFP located on the chloroplast and ER in plant cells. Overexpression of StGPT1 enhances late blight resistance in potato and Nicotiana benthamiana, activates immune responses including ROS bursts and up-regulation of PTI marker genes. The resistance function of StGPT1 seems to be related to its dual localization. Remarkably, StRFP1 ubiquitinates StGPT1 at the ER, possibly due to its merely transient function in peroxisomes, leading to apparent accumulation in chloroplasts. Our findings point to a novel mechanism by which a plant E3 ligase contributes to immunity via interacting with dually-targeted GPT1 at the ER of plant cells.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"52 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CGD: a multi-omics database for chrysanthemum genomic and biological research CGD：用于菊花基因组和生物学研究的多组学数据库

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-08-22 DOI: 10.1093/hr/uhae238

Jingxuan Ye, Chun Wang, Ye Liu, Shaocong Chen, Jinyu Jin, Lingling Zhang, Peixue Liu, Jing Tang, Jing Zhang, Zhenxing Wang, Jiafu Jiang, Su-Mei Chen, Fadi Chen, Aiping Song

{"title":"CGD: a multi-omics database for chrysanthemum genomic and biological research","authors":"Jingxuan Ye, Chun Wang, Ye Liu, Shaocong Chen, Jinyu Jin, Lingling Zhang, Peixue Liu, Jing Tang, Jing Zhang, Zhenxing Wang, Jiafu Jiang, Su-Mei Chen, Fadi Chen, Aiping Song","doi":"10.1093/hr/uhae238","DOIUrl":"https://doi.org/10.1093/hr/uhae238","url":null,"abstract":"Asteraceae is the largest family of dicotyledons and includes Chrysanthemum and Helianthus, two important genera of ornamental plants. The genus Chrysanthemum consists of more than 30 species and contains many economically important ornamental, medicinal and industrial plants. To more effectively promote Chrysanthemum research, we constructed the CGD, a Chrysanthemum genome database containing a large amount of data and useful tools. The CGD hosts well-assembled reference genome data for 6 Chrysanthemum species. These genomic data were fully annotated by comparison with various protein and domain data. Transcriptome data for nine different tissues, five flower developmental stages, and five treatments were subsequently added to the CGD. A fully functional “RNA data” module was designed to provide complete and visual expression profile data. In addition, the CGD also provides many of the latest bioinformatics analysis tools, such as the efficient sgRNA search tool for Chrysanthemum. In conclusion, the CGD provides the latest, richest, and most complete multi-omics resources and powerful tools for Chrysanthemum. Collectively, the CGD will become the central gateway for Chrysanthemum genomics and genetic breeding research and will aid in the study of polyploid evolution.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"3 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142042434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling pepper Immunity’s robustness to temperature shifts: insights for empowering future crops 揭示辣椒免疫对温度变化的适应能力：增强未来作物能力的启示

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-08-22 DOI: 10.1093/hr/uhae239

William Billaud, Judith Hirsch, Valentin Ribaut, Lucie Tamisier, Anne Massire, Marion Szadkowski, Félicie Lopez-Lauri, Benoît Moury, Véronique Lefebvre

{"title":"Unveiling pepper Immunity’s robustness to temperature shifts: insights for empowering future crops","authors":"William Billaud, Judith Hirsch, Valentin Ribaut, Lucie Tamisier, Anne Massire, Marion Szadkowski, Félicie Lopez-Lauri, Benoît Moury, Véronique Lefebvre","doi":"10.1093/hr/uhae239","DOIUrl":"https://doi.org/10.1093/hr/uhae239","url":null,"abstract":"Boosting plant immunity is an effective alternative to pesticides. However, environmental variations, accentuated by climate change, can compromise immunity. The robustness of a trait corresponds to the absence (or low level) of variation in that trait in the face of an environmental change. Here, we examined two types of robustness, robustness of immunity mean and robustness of immunity variation, and proposed nine quantitative robustness estimators. We characterized the immunity of a set of accessions representative of the natural diversity of pepper (Capsicum annuum L.), to two major pathogens: the oomycete Phytophthora capsici Leon. and potato virus Y. For each pathogen, we measured the immunity of accessions in two contrasting environments in terms of temperature. For each type of robustness and each pathogen, the impact of temperature change on immunity varied between accessions. The robustness estimators proved to be complementary and differed in terms of heritability and ability to discriminate between accessions. A positive and significant correlation was observed between immunity and robustness. There was no significant relationship between the robustness of immunity to the two pathogens, but some accessions showed high immunity and robustness against both pathogens. These results justify the need to consider both immunity and robustness to environmental variations in order to select varieties adapted to current and future climate conditions. Phenotypic robustness should also be considered when assessing the “value of sustainable cultivation and use” (VSCU) of future plant varieties, particularly during the application process for protection rights granted from the European Community Plant Variety Office (CPVO).","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"49 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142042435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inference of the genetic basis of fruit texture in highbush blueberries using genome-wide association analyses 利用全基因组关联分析推断高丛蓝莓果实质地的遗传基础

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-08-22 DOI: 10.1093/hr/uhae233

Luis Felipe V Ferrão, Camila Azevedo, Juliana Benevenuto, Molla Fentie Mengist, Claire Luby, Marti Pottorff, Gonzalo I P Casorzo, Ted Makey, Mary Ann Lila, Lara Giongo, Nahla Bassil, Penelope Perkins-Veazie, Massimo Iorizzo, Patricio R Munoz

{"title":"Inference of the genetic basis of fruit texture in highbush blueberries using genome-wide association analyses","authors":"Luis Felipe V Ferrão, Camila Azevedo, Juliana Benevenuto, Molla Fentie Mengist, Claire Luby, Marti Pottorff, Gonzalo I P Casorzo, Ted Makey, Mary Ann Lila, Lara Giongo, Nahla Bassil, Penelope Perkins-Veazie, Massimo Iorizzo, Patricio R Munoz","doi":"10.1093/hr/uhae233","DOIUrl":"https://doi.org/10.1093/hr/uhae233","url":null,"abstract":"The global production and consumption of blueberry (Vaccinium spp.), a specialty crop known for its abundant bioactive and antioxidant compounds, has more than doubled over the last decade. To hold this momentum, plant breeders have begun to use quantitative genetics and molecular breeding to guide their decisions and select new cultivars that are improved for fruit quality. In this study, we leveraged our inferences on the genetic basis of fruit texture and chemical components by surveying large breeding populations from northern (NHB) and southern (SHB) highbush blueberries, the two dominant cultivated blueberries. After evaluating 1065 NHB genotypes planted at the Oregon State University, and 992 SHB genotypes maintained at the University of Florida for 20 texture-related traits, evaluated over multiple years, our contributions consist of: (i) We drew attention to differences between NHB and SHB materials and showed that both blueberry types can be differentiated using texture traits; (ii) We computed genetic parameters and shed light on the genetic architecture of important texture attributes, indicating that most traits had a complex nature with low to moderate heritability; (iii) using molecular breeding, we emphasized that prediction could be performed across populations; and finally (iv) the genomic-association analyses pinpointed some genomic regions harboring potential candidate genes for texture that could be used for further validation studies. Altogether, the methods and approaches used here can guide future breeding efforts focused on maximizing texture improvements in blueberries.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"17 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142042481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial transcriptome analysis reveals de novo regeneration of poplar roots 空间转录组分析揭示了杨树根系的新生

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-08-21 DOI: 10.1093/hr/uhae237

Kaiwen Lv, Naixu Liu, Yani Niu, Xiehai Song, Yongqi Liu, Zhiliang Yue, Muhammad Ali, Qiuyue Guo, Chunyu Lv, Dongdong Lu, Shaoman Zhang, Yangyan Zhou, Bosheng Li

{"title":"Spatial transcriptome analysis reveals de novo regeneration of poplar roots","authors":"Kaiwen Lv, Naixu Liu, Yani Niu, Xiehai Song, Yongqi Liu, Zhiliang Yue, Muhammad Ali, Qiuyue Guo, Chunyu Lv, Dongdong Lu, Shaoman Zhang, Yangyan Zhou, Bosheng Li","doi":"10.1093/hr/uhae237","DOIUrl":"https://doi.org/10.1093/hr/uhae237","url":null,"abstract":"Propagation through cuttings is a well-established and effective technique for plant multiplication. This study explores the regeneration of poplar roots using spatial transcriptomics to map a detailed developmental trajectory. Mapping of the time-series transcriptome data revealed notable alterations in gene expression during root development, particularly in the activation of cytokinin-responsive genes. Our analysis identified six distinct clusters during the second and third stages, each corresponding to specific anatomical regions with unique gene expression profiles. Auxin response cis-elements (AuxREs) were prevalent in the promoters of these cytokinin-responsive genes, indicating a regulatory interplay between auxin and cytokinin. Pseudo-temporal trajectory analysis mapped the differentiation from cambium cells to root primordium cells, revealing a complex pattern of cell differentiation. SAC56 and LOS1 emerged as potential novel biomarkers for enhancing root regeneration, with distinct spatial expression patterns confirmed by in situ hybridization. This comprehensive spatial analysis enhances our understanding of the molecular interactions driving root regeneration and provides insights for improving plant propagation techniques.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"4 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142022052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CsTs, a C-type lectin receptor-like kinase, regulates the development trichome development and cuticle metabolism in cucumber (Cucumis sativus) C型凝集素受体样激酶CsTs调控黄瓜（Cucumis sativus）的毛状体发育和角质层代谢

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-08-14 DOI: 10.1093/hr/uhae235

Duo Lv, Haifan Wen, Gang Wang, Juan Liu, Chunli Guo, Jingxian Sun, Keyan Zhang, ChaoHan Li, Jiaqi You, Ming Pan, Huanle He, Run Cai, Junsong Pan

{"title":"CsTs, a C-type lectin receptor-like kinase, regulates the development trichome development and cuticle metabolism in cucumber (Cucumis sativus)","authors":"Duo Lv, Haifan Wen, Gang Wang, Juan Liu, Chunli Guo, Jingxian Sun, Keyan Zhang, ChaoHan Li, Jiaqi You, Ming Pan, Huanle He, Run Cai, Junsong Pan","doi":"10.1093/hr/uhae235","DOIUrl":"https://doi.org/10.1093/hr/uhae235","url":null,"abstract":"Cucumber (Cucumis sativus) fruit spines are a classic material for researching the development of multicellular trichomes. Some key genes that influence trichome development have been confirmed to be associated with cuticle biosynthesis and secondary metabolism. However, the biological mechanisms underlying trichome development, cuticle biosynthesis, and secondary metabolism in cucumber remain poorly understood. Cucumber (Cucumis sativus) fruit spines are classic material for researching the development of multicellular trichomes. Some key genes that influence trichome development have been confirmed to be associated with cuticle biosynthesis and secondary metabolism. However, the biological mechanisms underlying trichome development, cuticle biosynthesis, and secondary metabolism in cucumber remain poorly understood. CsTs, a C-type lectin receptor-like kinase gene, reportedly causes a tender trichome phenotype in cucumber when it mutates. In this study, the role of CsTs in cucumber fruit spine morphogenesis was confirmed using gene editing technology. Sectioning technology and cell wall component detection were used to analyze the main causes of tender fruit spines in the ts mutant. Subsequently, transcriptome data and a series of molecular biology experiments were used to further investigate the relationship between CsTs and cytoskeletal homeostasis in cucumber. CsTs overexpression partially compensated for the abnormal trichome phenotype of an Arabidopsis homolog mutant. Genetic hybridization and metabolic analysis indicated that CsTs and CsMcit can affect trichome development and cuticle biosynthesis using the same pathway. Our findings provide important background information for future research on the molecular mechanism underlying cucumber trichome development and contribute to understanding the biological function of C-type lectin receptor-like kinases.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"23 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141986214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histone deacetylase SlHDA7 impacts fruit ripening and shelf life in tomato 组蛋白去乙酰化酶 SlHDA7 对番茄果实成熟和货架期的影响

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-08-14 DOI: 10.1093/hr/uhae234

Yijie Zhou, Zhiwei Li, Xinguo Su, Huiyu Hou, Yueming Jiang, Xuewu Duan, Hongxia Qu, Guoxiang Jiang

{"title":"Histone deacetylase SlHDA7 impacts fruit ripening and shelf life in tomato","authors":"Yijie Zhou, Zhiwei Li, Xinguo Su, Huiyu Hou, Yueming Jiang, Xuewu Duan, Hongxia Qu, Guoxiang Jiang","doi":"10.1093/hr/uhae234","DOIUrl":"https://doi.org/10.1093/hr/uhae234","url":null,"abstract":"Fruit ripening depends on the accurate control of ripening-related genes expression, with histone deacetylases (HDACs) playing crucial roles in transcriptional regulation. However, the functions of HDACs in fruit maturation remain largely unexplored. Here, we show that SlHDA7 acts as a suppressor of fruit ripening and functions as an H4ac HDAC in tomato. Deletion of SlHDA7 accelerated fruit ripening, while overexpression of SlHDA7 delayed maturation process. Additionally, ethylene production and carotenoid biosynthesis significantly increased in slhda7 mutant fruits but decreased in SlHDA7-overexpressing fruits. Furthermore, SlHDA7 repress the expression of ethylene production and signaling, carotenoid metabolism, cell wall modification, and transcriptional regulation-related genes. RT-qPCR and ChIP-qPCR analyses indicated that SlHDA7 may deacetylate H4ac, leading to reduced transcript levels of ACO1, GGPPS2, Z-ISO, EXP1, and XYL1 mRNA, consequently suppressing fruit ripening. Moreover, SlHDA7 suppresses fruit ripening by targeting specific ripening-associated transcription factors (TFs) like RIN, FUL1, and ERF.E1, ultimately leading to delayed ripening and prolonged fruit shelf life. In summary, our findings indicate that SlHDA7 negatively modulates tomato fruit maturation by adjusting H4ac levels of these ripening-associated genes and key TFs.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"20 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141986213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotype-associated core bacteria enhance host resistance against kiwifruit bacterial canker 基因型相关核心菌增强宿主对猕猴桃细菌性腐烂病的抵抗力

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-08-13 DOI: 10.1093/hr/uhae236

Min Fu, Yunhe Chen, Yong-Xin Liu, Xiaoxi Chang, Lei Zhang, Xinyi Yang, Li Li, Lixin Zhang

{"title":"Genotype-associated core bacteria enhance host resistance against kiwifruit bacterial canker","authors":"Min Fu, Yunhe Chen, Yong-Xin Liu, Xiaoxi Chang, Lei Zhang, Xinyi Yang, Li Li, Lixin Zhang","doi":"10.1093/hr/uhae236","DOIUrl":"https://doi.org/10.1093/hr/uhae236","url":null,"abstract":"Both the phyllosphere and rhizosphere are inhabited by different kinds of microorganisms that are closely related to plant growth and health. However, it is not clear whether disease-resistant cultivars shape the microbiome to facilitate disease resistance. In this study, significant differences were found in the aboveground and belowground bacterial communities of disease-resistant and disease-susceptible cultivars grown in the same kiwifruit orchard. The phyllosphere of the resistant cultivar “Wanjin” was more strongly affected by geographic factors and showed greater enrichment of Pseudomonas spp. and Sphingomonas spp. than the susceptible cultivar “Donghong”. The rhizosphere microbes of “Wanjin” were less affected by field location, with significantly greater bacterial abundance, than those of “Donghong”, and more bacteria with potential biocontrol properties. Pseudomonas syringae pv. actinidiae (Psa) infection significantly affected the microbiome of the phyllosphere of kiwifruit plants, especially that of “Donghong”. Resistant and susceptible kiwifruit cultivars exhibit distinct beneficial microbial recruitment strategies under Psa challenge. The phyllosphere of “Donghong” in Jinzhai was enriched with Sphingomonas spp. and Pantoea spp. under Psa infection, while the rhizosphere of “Wanjin” was enriched with Sphingomonas spp. and Novosphingobium spp. We further identified five key biomarkers within the microbial community associated with Psa infection. Detached-branch inoculation experiments showed that Lysobacter sp. R34, Stenotrophomonas sp. R31, Pseudomonas sp. R10 and RS54, which were isolated from the root endosphere or rhizosphere of “Wanjin”, could positively affect plant performance under Psa challenge. Our findings provided novel insights into soil–microbe–plant interactions and the role of microbes in plant disease resistance and susceptibility.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"48 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141986215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide association study identifies the genetic basis of key agronomic traits in 207 sugar beet accessions 全基因组关联研究确定了 207 个甜菜品种关键农艺性状的遗传基础

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-08-12 DOI: 10.1093/hr/uhae230

Sufang Wang, Zhiyong Yue, Chao Yu, Ruili Wang, Yang Sui, Yaguang Hou, Ying Zhao, Lingling Zhao, Chunmei Chen, Zhimin Yang, Ke Shao

引用次数: 0

Fine mapping and identification of candidate genes associated with powdery mildew resistance in melon (Cucumis melo L.) 甜瓜（Cucumis melo L.）抗白粉病候选基因的精细图谱绘制与鉴定

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-08-12 DOI: 10.1093/hr/uhae222

Xiaoyu Duan, Yue Yuan, Núria Real, Mi Tang, Jian Ren, Jiaqi Wei, Bin Liu, Xuejun Zhang

{"title":"Fine mapping and identification of candidate genes associated with powdery mildew resistance in melon (Cucumis melo L.)","authors":"Xiaoyu Duan, Yue Yuan, Núria Real, Mi Tang, Jian Ren, Jiaqi Wei, Bin Liu, Xuejun Zhang","doi":"10.1093/hr/uhae222","DOIUrl":"https://doi.org/10.1093/hr/uhae222","url":null,"abstract":"Powdery mildew, a common disease of many major crop species, including melon (Cucumis melo L.), affects plant growth and fruit quality, and seriously reduces production. Using a combined morphological and molecular approach, we attribute the powdery mildew pathogen that naturally occurs in melon to Podosphaera xanthii, and specifically to physiological race 1. An investigation into the genetic basis of powdery mildew resistance in melon using the resistant accession “PI 164637” and susceptible counterpart “HDZ” reveals dominant inheritance of PM resistance at the seedling stage, supported by F2 and backcross population segregation ratios. Adult plant assessments indicate a major gene with an additive effect for powdery mildew resistance. Bulk segregant analysis coupled with high-throughput sequencing identified a significant quantitative trait locus on chromosome 6 that is associated with powdery mildew resistance. Genetic mapping narrowed down the candidate region to 63.5 kb using InDel molecular markers, harboring 12 candidate genes. The marker chr06_indel_5047127 demonstrated high accuracy in screening powdery mildew resistance in a F2 segregating population and 30 inbred lines as natural populations. Functional annotation and expression analysis of candidate genes revealed that MYB transcription factor MELO3C006700, GATA transcription factor MELO3C028829 and heparanase-like protein MELO3C006697 are promising candidate genes for powdery mildew resistance in melon. The genetic architecture underlying this resistance in melon offers valuable insights for breeding programs, and the identified markers, especially chr06_indel_5047127, may enable practical applications for marker-assisted selection in developing powdery-mildew-resistant melon varieties.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"2013 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0