Histology and histopathology最新文献

筛选
英文 中文
NTRK protein expression in NSCLC and its clinicopathological correlation. NTRK蛋白在非小细胞肺癌中的表达及其临床病理相关性
IF 2.5 4区 生物学
Histology and histopathology Pub Date : 2025-02-26 DOI: 10.14670/HH-18-894
Jair Gutierrez-Herrera, M Angeles Montero-Fernandez, Luigi De Petris, Ricardo Guijarro, Simon Ekman, Cristian Ortiz-Villalón
{"title":"NTRK protein expression in NSCLC and its clinicopathological correlation.","authors":"Jair Gutierrez-Herrera, M Angeles Montero-Fernandez, Luigi De Petris, Ricardo Guijarro, Simon Ekman, Cristian Ortiz-Villalón","doi":"10.14670/HH-18-894","DOIUrl":"10.14670/HH-18-894","url":null,"abstract":"<p><p>Non-small cell lung cancer (NSCLC) is a complex disease with diverse clinical and molecular characteristics. Since the discovery of the oncogenic neurotrophic receptor tyrosine kinase (NTRK) gene fusion in colorectal cancer in 1986, its understanding has gradually progressed. NTRK's relevance is crucial to understanding some tumor development and how specific tyrosine receptor kinase inhibitors (TRKI) work. <i>NTRK1, NTRK2,</i> and <i>NTRK3</i> (encoding the neurotrophin receptors TRKA, TRKB, and TRKC, respectively) rearrangement induces high tumor transforming ability. This study investigates the clinical relevance of NTRK immunohistochemistry in NSCLC patients in a cohort of 482 patients from Karolinska University Hospital with detailed clinical and pathological studies. Immunohistochemical staining for NTRK expression was performed, and the results were correlated with patient demographics, histological subtypes, tumor extent, and staging. Our findings revealed that NTRK expression, predominantly membranous and cytoplasmic, was detected in 22 out of 482 cases (4.56%). Notably, NTRK expression was more frequently observed in squamous-cell carcinoma (SqCC) than in adenocarcinoma (AdCa). Clinical correlations indicated a significant association between NTRK expression and histologic subtypes (<i>p</i><0.001) and grade of differentiation (<i>p</i>=0.036). However, no significant correlations were observed with age, sex, tumor size, staging, or OS. NTRK immunohistochemistry represents a potential biomarker for a subset of NSCLC patients, particularly those with SqCC histology. Understanding the clinical implications of NTRK expression may contribute to personalized treatment strategies in NSCLC.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18894"},"PeriodicalIF":2.5,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pinocembrin ameliorates non-alcoholic fatty liver disease by activating Nrf2/HO-1 and inhibiting the NF-κB signaling pathway. 匹诺曹通过激活Nrf2/HO-1和抑制NF-κB信号通路改善非酒精性脂肪性肝病。
IF 2.5 4区 生物学
Histology and histopathology Pub Date : 2025-02-25 DOI: 10.14670/HH-18-893
Weina Chen, Diming Xue, Xia Feng, Yinhang Zhong, Quanqing Li, Weihang Zhang, Guojun Jiang
{"title":"Pinocembrin ameliorates non-alcoholic fatty liver disease by activating Nrf2/HO-1 and inhibiting the NF-κB signaling pathway.","authors":"Weina Chen, Diming Xue, Xia Feng, Yinhang Zhong, Quanqing Li, Weihang Zhang, Guojun Jiang","doi":"10.14670/HH-18-893","DOIUrl":"https://doi.org/10.14670/HH-18-893","url":null,"abstract":"<p><strong>Objectives: </strong>The high intake of high-fat diets and changes in sedentary lifestyles have led to an increase in non-alcoholic fatty liver disease (NAFLD). This study aimed to investigate the effect and mechanism of Pinocembrin (Pin) on NAFLD <i>in vivo</i> and <i>in vitro</i>.</p><p><strong>Methods: </strong>The pharmacodynamics of Pin alone or in combination with ML385 was assessed in high-fat diet (HFD)-mediated NAFLD mice. HepG2 cells were treated with palmitic acid (PA)/oleic acid (OA) (1:2) as an <i>in-vitro</i> model to study the effect of Pin on lipid deposition and oxidative stress. The roles of Pin in glucose and lipid metabolism, inflammation, oxidative stress, and the Nrf2/HO-1/NF-κB pathway were measured.</p><p><strong>Results: </strong>Pin alleviated lipid deposition, inflammatory response, and oxidative stress in HFD-induced NAFLD mice and PA/OA-induced HepG2 cells. Moreover, ML385 partly attenuated the protection of Pin on inflammatory response and oxidative stress <i>in vivo</i> and <i>in vitro</i>. More importantly, feeding with an HFD significantly decreased the expression of Nrf2 and HO-1, but treatment with Pin increased their expression, accompanied by an increased nuclear transposition of Nrf2.</p><p><strong>Conclusion: </strong>Taken together, these results indicated that Pin alleviated glucose and lipid metabolism disorders, inflammation, and oxidative stress in NAFLD by activating the Nrf2/HO-1 signaling pathway and restraining the NF-κB pathway.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18893"},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chelerythrine-mediated targeting of NF-κB and Nrf2 pathways alleviates liver injury in a carbon tetrachloride-induced liver fibrosis mouse model. 车车草碱介导的NF-κB和Nrf2通路靶向减轻四氯化碳诱导的肝纤维化小鼠模型的肝损伤。
IF 2.5 4区 生物学
Histology and histopathology Pub Date : 2025-02-24 DOI: 10.14670/HH-18-892
Yisong Ding, Xiaoming Li, Ruixing Qi, Yingshi Su, Xiaoli Wang
{"title":"Chelerythrine-mediated targeting of NF-κB and Nrf2 pathways alleviates liver injury in a carbon tetrachloride-induced liver fibrosis mouse model.","authors":"Yisong Ding, Xiaoming Li, Ruixing Qi, Yingshi Su, Xiaoli Wang","doi":"10.14670/HH-18-892","DOIUrl":"10.14670/HH-18-892","url":null,"abstract":"<p><strong>Background and aims: </strong>This study aimed to investigate the mechanism and efficacy of chelerythrine (CHE) in treating carbon tetrachloride (CCl<sub>4</sub>)-induced liver fibrosis, with a particular focus on the nuclear factor-erythroid-related factor-2 (Nrf2) and nuclear factor-kappa-B (NF-κB) signaling pathways.</p><p><strong>Methods: </strong>Mice were induced with CCl<sub>4</sub> for eight weeks and categorized into the control group, CCl<sub>4</sub> model group, and CHE low (7 mg/kg/d, ig,), medium (14 mg/kg/d, ig), and high-dose (28 mg/kg/d, ig) groups with 10 animals in each group. Following CHE treatment, liver sample morphology was assessed using multiple immunohistochemistry, and serum biochemical indicators were measured. ELISA was used to determine IL-10, IL-1β, and TNF-α contents. Western blotting and RT-PCR were employed to analyze protein and mRNA levels of α-SMA, Col-I, fibronectin, Nrf2, HO-1, NQO1, GCLc, GCLm, NF-κB, p-NF-κB, IκBα, and p-IκBα. Nrf2 knockout mice were used to assess the impact of CHE on the Nrf2 signaling pathway.</p><p><strong>Results: </strong>The findings demonstrated that CHE significantly ameliorated oxidative damage, inflammatory response, and liver fibrosis in CCl<sub>4</sub>-induced mice. CHE treatment increased Nrf2 expression and its target proteins, including HO-1 and GCLc, an effect not observed in Nrf2 knockout mice. In addition, CHE reduced NF-κB expression levels.</p><p><strong>Conclusions: </strong>These results suggest that CHE can alleviate liver fibrosis in CCl<sub>4</sub>-induced mice by modulating NF-κB/IκBα and Nrf2 signaling pathways. These findings propose CHE as a potential novel anti-liver fibrosis drug.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18892"},"PeriodicalIF":2.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Adaptive changes in the visual cortex after photoreceptor degeneration in retinitis pigmentosa. 色素性视网膜炎光感受器变性后视皮层的适应性变化。
IF 2.5 4区 生物学
Histology and histopathology Pub Date : 2025-02-21 DOI: 10.14670/HH-18-891
Juan R Martinez-Galan, Elena Caminos
{"title":"Adaptive changes in the visual cortex after photoreceptor degeneration in retinitis pigmentosa.","authors":"Juan R Martinez-Galan, Elena Caminos","doi":"10.14670/HH-18-891","DOIUrl":"https://doi.org/10.14670/HH-18-891","url":null,"abstract":"<p><p>Retinitis pigmentosa (RP) is a group of hereditary disorders that cause progressive retinal degeneration, affecting the rods and, subsequently, the cones, which results in progressive vision loss. RP is genetically heterogeneous and is inherited in an autosomal dominant, autosomal recessive, X-linked, or sporadic non-Mendelian manner. The recent advancements in repairing damaged retinas highlight the necessity of understanding the impact of photoreceptor degeneration on the visual cortex. This is because functional vision may not be adequately restored if this region is significantly impaired prior to treatment. In the present review, we have analyzed the rodent models of RP that have been most frequently used and the physiological and morphological changes occurring in both humans and rodents with this disorder. Following visually evoked stimulation, the processing of visual information in the primary visual cortex (V1) of individuals with RP is altered due to modifications in the transduction of the signal originating in the degenerated retina. Moreover, alterations in the intrinsic electrophysiological properties of cortical neurons and neural circuits have also been documented. Finally, several neurochemical and/or morphological changes are observed in synaptic structures associated with pyramidal neurons and in select inhibitory interneurons. Nevertheless, despite the physiological and morphological changes that have been described, the impact of RP on the visual cortex does not inevitably result in irreversible damage, as the alterations do not appear to be particularly severe. Brain plasticity is more restricted in adults; however, remodeling of the visual cortex in mice and humans is possible, which encourages further work on therapies capable of partially restoring the lost visual function.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18891"},"PeriodicalIF":2.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The role of neutrophils and their immunosuppressive effects on prostate cancer. 中性粒细胞在前列腺癌中的作用及其免疫抑制作用。
IF 2.5 4区 生物学
Histology and histopathology Pub Date : 2025-02-20 DOI: 10.14670/HH-18-890
Yihaoyun Lou, Zhiguo Fan, Shancheng Ren
{"title":"The role of neutrophils and their immunosuppressive effects on prostate cancer.","authors":"Yihaoyun Lou, Zhiguo Fan, Shancheng Ren","doi":"10.14670/HH-18-890","DOIUrl":"https://doi.org/10.14670/HH-18-890","url":null,"abstract":"<p><p>Over the past decade, there has been a significant increase in the incidence of prostate cancer on a global scale, establishing it as the second most prevalent malignant tumor among European and American males. Neutrophils are myeloid immune cells that constitute a crucial proportion of inflammatory cells within the human circulatory system and actively contribute to the composition of the prostate tumor microenvironment (TME). Recent investigations have revealed that neutrophils are influenced by the surrounding tumor environment and possess a dual capacity to either promote or suppress cancer within the TME. Nevertheless, the precise mechanisms of neutrophils in prostate cancer remain inadequately elucidated. In this review, we aimed to comprehensively outline the character of neutrophils in the development and progression of prostate cancer while also exploring the clinical prognostic significance of the neutrophil-to-lymphocyte ratio (NLR).</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18890"},"PeriodicalIF":2.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Suppressive role of fukutin on cell proliferation in uterine cervical carcinoma in relation to Aurora-A kinase. 福库汀对子宫颈癌细胞增殖的抑制作用与 Aurora-A 激酶的关系
IF 2.5 4区 生物学
Histology and histopathology Pub Date : 2025-02-19 DOI: 10.14670/HH-18-889
Tomoko Yamamoto, Yukinori Okamura, Yoichiro Kato, Kenta Masui, Yoji Nagasima, Atsushi Kurata
{"title":"Suppressive role of fukutin on cell proliferation in uterine cervical carcinoma in relation to Aurora-A kinase.","authors":"Tomoko Yamamoto, Yukinori Okamura, Yoichiro Kato, Kenta Masui, Yoji Nagasima, Atsushi Kurata","doi":"10.14670/HH-18-889","DOIUrl":"https://doi.org/10.14670/HH-18-889","url":null,"abstract":"<p><p><i>Fukutin</i>, the gene responsible for Fukuyama congenital muscular dystrophy (FCMD), is involved in the glycosylation of α-dystroglycan (α-DG). On the other hand, fukutin is expressed in various organs, and the roles of fukutin in non-neuromuscular tissues are not fully elucidated. In the present immunohistochemical study of uterine cervical carcinoma, Ki-67-positive cells tended to be more in areas showing weaker expression of fukutin. In HeLa cells, Ki-67-positive cells were increased after the suppression of fukutin by RNAi, and were decreased by overexpression of fukutin. Similar results were obtained upon phosphorylation of histone H3 at serine 10, which is enriched during mitosis. Interestingly, Aurora-A kinase (AURKA), one of the proteins regulating Ser10 phosphorylation of histone H3, was highly expressed in HeLa cells, and the phosphorylation of AURKA was reduced by overexpression of fukutin. Furthermore, fukutin is co-localized with targeting protein for Xklp2 (TPX2), a protein enhancing AURKA activity. Fukutin may be able to suppress cell proliferation by reducing AURKA phosphorylation, probably competing with TPX2.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18889"},"PeriodicalIF":2.5,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advantages and limitations of 3,3',5,5'-tetramethylbenzidine for immunohistochemical staining. 3,3',5,5'-四甲基联苯胺免疫组化染色的优点与局限性。
IF 2.5 4区 生物学
Histology and histopathology Pub Date : 2025-02-18 DOI: 10.14670/HH-18-888
Chao Yu, Xiao Liu, Peiyuan Zhao, Zhidong Sun, Yurong Song, Yuan Cao, Ming Cheng
{"title":"Advantages and limitations of 3,3',5,5'-tetramethylbenzidine for immunohistochemical staining.","authors":"Chao Yu, Xiao Liu, Peiyuan Zhao, Zhidong Sun, Yurong Song, Yuan Cao, Ming Cheng","doi":"10.14670/HH-18-888","DOIUrl":"10.14670/HH-18-888","url":null,"abstract":"<p><p>In this study, two chromogenic systems, horseradish peroxidase (HRP)-3,3'-diaminobenzidine (DAB) and HRP-3,3',5,5'-tetramethylbenzidine (TMB), were used to perform single-color and double-color immunohistochemical staining (sIHC and dIHC, respectively) on multiple antigens in four distinct tissue types. The chromogenic results of the HRP-TMB system exhibited a vibrant blue-green color, and the tissue localization and signal intensity were consistent with those of the HRP-DAB system. In addition, it demonstrated clear differentiation from the hematoxylin-stained nucleus, endogenous melanin, and brown chromogenic results of HRP-DAB. TMB staining in tissues that contain high endogenous pigment levels eliminates the need for melanin bleaching, thereby facilitating direct observation and potentially improving the detection speed and interpretation. TMB can also be used in combination with DAB for dIHC, thus allowing detection of the two markers on a single slide. However, the TMB staining results are not stable over the long term and require image storage using slide scanners, thereby limiting its application. Additionally, in dIHC, overlapping signals of the first marker may obscure the second marker, potentially leading to bias or false negatives. Therefore, careful consideration is required when designing dIHC detection systems. Based on the above, we propose that TMB is a valuable supplement to DAB for immunohistochemical staining and deserves further promotion and utilization. However, additional research is needed to improve the composition of TMB chromogenic reagents, prolong the longevity of staining results, and overcome current limitations.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18888"},"PeriodicalIF":2.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genomic features of bladder neuroendocrine carcinoma with composite histology. 复合组织学膀胱神经内分泌癌的基因组特征。
IF 2.5 4区 生物学
Histology and histopathology Pub Date : 2025-02-17 DOI: 10.14670/HH-18-887
Akihiro Ohmoto, Keiichiro Kitahama, Yasuyuki Shigematsu, Naomi Hayashi, Junji Yonese, Kentaro Inamura, Shunji Takahashi
{"title":"Genomic features of bladder neuroendocrine carcinoma with composite histology.","authors":"Akihiro Ohmoto, Keiichiro Kitahama, Yasuyuki Shigematsu, Naomi Hayashi, Junji Yonese, Kentaro Inamura, Shunji Takahashi","doi":"10.14670/HH-18-887","DOIUrl":"https://doi.org/10.14670/HH-18-887","url":null,"abstract":"<p><p>Neuroendocrine carcinoma (NEC) is a rare and aggressive malignancy derived from multiple body parts, with the urogenital organs being the second-largest extrapulmonary sites. The detailed mechanism of bladder NEC pathogenesis remains unknown. We reviewed data from 23 patients diagnosed with NEC from urogenital organs (bladder and prostate) and conducted targeted sequencing of 523 cancer-related genes, focusing on bladder NEC. While 14 cases featured a pure NEC histology, the remaining nine cases included NEC histology mixed with other tumors, such as urothelial carcinoma (UC) or adenocarcinoma. Median overall survival in the entire cohort was 11.1 months, and survival curves were comparable between pure NEC and NEC of mixed appearance. Major mutations detected in the NEC component were in <i>TP53</i> (38%), <i>TERT</i> promoter (31%), <i>PIK3CA</i> (25%), histone-modification genes (19%), and <i>RB1</i> (19%). The <i>BARD1</i> frameshift variant related to homologous recombination was also detected in one patient. More than half of the patients had a high total mutational burden (TMB; ≥10), including two with a TMB ≥45. Intriguingly, at least one identical gene variant in driver genes was detected between NEC and non-NEC (UC) components in the four bladder specimens analyzed. These results highlight the possibility of shared genetic background between bladder NEC and UC. Additionally, several cases harbored druggable gene alterations as presented by TMB-high. Our presentation of the histopathological and molecular features of NEC may help clarify the underlying mechanisms and contribute to efficient treatment of the disease.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18887"},"PeriodicalIF":2.5,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dian sanguis draconis improves pulmonary fibrosis by activating autophagy to regulate the PA/PAI-1 balance. 地血通过激活自噬调节PA/PAI-1平衡改善肺纤维化。
IF 2.5 4区 生物学
Histology and histopathology Pub Date : 2025-02-14 DOI: 10.14670/HH-18-886
Jiayi Song, Qian Li, Chunyan Yang, Qing Liu, Jianmei Li, Yi Fu
{"title":"Dian sanguis draconis improves pulmonary fibrosis by activating autophagy to regulate the PA/PAI-1 balance.","authors":"Jiayi Song, Qian Li, Chunyan Yang, Qing Liu, Jianmei Li, Yi Fu","doi":"10.14670/HH-18-886","DOIUrl":"https://doi.org/10.14670/HH-18-886","url":null,"abstract":"<p><strong>Background: </strong>Pulmonary fibrosis (PF) is a severe lung disease that manifests as lung tissue destruction and collagen deposition and easily leads to respiratory failure. It is difficult to reverse these conditions using current treatment methods. This study focused on the exploration and development of novel drugs for the treatment of PF.</p><p><strong>Methods: </strong>This study simulated the pathological process of PF by using a bleomycin (BLM)-induced rat model and a TGF-β1-induced in vitro cell model. Dian sanguis draconis (DSD) was used for intervention, and the effects on the lung tissue structure, collagen fiber deposition, autophagy level and PA/PAI-1 balance were evaluated via pathological tissue staining, western blotting, and ELISA.</p><p><strong>Results: </strong>In untreated PF rats, severely disordered lung tissue, thickened alveolar septa, and excessive deposition of collagen fibers were observed. In addition, the level of autophagy was inhibited, and the balance of PA/PAI-1 in the lung tissue was disrupted. After treatment with DSD, these pathological injuries improved, as demonstrated by the restoration of lung tissue structure, reduction in collagen fiber deposition, recovery of autophagy levels, and remodeling of the PA/PAI-1 balance. In addition, mechanistically, DSD improves PF by increasing the level of autophagy-related proteins and regulating the PA/PAI-1 balance.</p><p><strong>Conclusion: </strong>This study confirmed the significant effect of DSD in alleviating PF. These findings provide new drug candidates for the treatment of PF.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18886"},"PeriodicalIF":2.5,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Shensiqigui tablets alleviate bleomycin-induced pulmonary fibrosis by inhibiting the hedgehog/wnt-β-catenin pathway. 参四芪龟片通过抑制hedgehog/wnt-β-catenin通路减轻博来霉素诱导的肺纤维化。
IF 2.5 4区 生物学
Histology and histopathology Pub Date : 2025-02-13 DOI: 10.14670/HH-18-885
Rui Ma, Yupeng Xie, Yuan Dong, Fan Yin, Jiong Yang, Fenghua Xu
{"title":"Shensiqigui tablets alleviate bleomycin-induced pulmonary fibrosis by inhibiting the hedgehog/wnt-β-catenin pathway.","authors":"Rui Ma, Yupeng Xie, Yuan Dong, Fan Yin, Jiong Yang, Fenghua Xu","doi":"10.14670/HH-18-885","DOIUrl":"https://doi.org/10.14670/HH-18-885","url":null,"abstract":"<p><strong>Background: </strong>Pulmonary fibrosis (PF) is a refractory disease characterized by inflammation and fibrosis. Shensiqigui Tablets (SSQGT), a combination of Codonopsis pilosula, Astragalus, and Angelica, is a traditional Chinese medicine with anti-inflammatory and antioxidant properties. Therefore, SSQGT may be a potential therapeutic agent for managing PF. This study aimed to investigate the effects of SSQGT on PF and its potential mechanisms.</p><p><strong>Methods: </strong>This study established a mouse model of PF through a single intratracheal injection of bleomycin (BLM) and used a TGF-β1-induced HFL-1 cell model. The experiment included control, model (BLM/TGF-β1), and treatment groups (pirfenidone, compound Biejiaruangan tablet (BJRGT), low-dose SSQGT, medium-dose SSQGT, and high-dose SSQGT). Histopathological changes and collagen deposition in lung tissues were observed using Hematoxylin-Eosin (HE) and Masson staining. Inflammatory exudation in bronchoalveolar lavage fluid (BALF) was assessed using ELISA, including TNF-α, IL-1β, IL-6, and NO. Oxidative stress markers SOD, GSH, and Malondialdehyde (MDA) were measured using commercial kits. mRNA and protein expression levels in lung tissues and <i>in vitro</i> models, including α-SMA, vimentin, collagen I, caspase-3, TGF-β, and the Hedgehog/Wnt-β-catenin pathway, were evaluated using qRT-PCR and western blot analysis.</p><p><strong>Results: </strong>SSQGT significantly alleviated BLM-induced weight loss and lung injury in mice and reduced HYP levels and collagen deposition. Additionally, SSQGT improved oxidative stress markers (decreased MDA levels and increased SOD and GSH activity) and mitigated inflammatory responses (reduced TNF-α, IL-1β, IL-6, and NO levels) and (downregulated α-SMA, collagen I, caspase-3, and TGF-β). Further mechanistic analysis showed that SSQGT inhibited the Hedgehog/Wnt-β-catenin pathway.</p><p><strong>Conclusion: </strong>SSQGT alleviates BLM- or TGF-β1-induced PF by reducing oxidative stress and inhibiting inflammation through the suppression of the Hedgehog/Wnt-β-catenin pathway, suggesting its potential as a therapeutic agent for PF.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18885"},"PeriodicalIF":2.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信