Histology and histopathology最新文献_第10页

MLH1 and MSH2 expression in endometrial cancer - microscopic and computer assessment of immunohistochemical method. 子宫内膜癌中MLH1和MSH2的表达-免疫组织化学方法的显微镜和计算机评价。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-02-06 DOI: 10.14670/HH-18-884

Jan Rogaliński, Stanisław Przewoźny, Mateusz de Mezer, Anna Markowska, Janina Markowska, Jakub Żurawski

{"title":"MLH1 and MSH2 expression in endometrial cancer - microscopic and computer assessment of immunohistochemical method.","authors":"Jan Rogaliński, Stanisław Przewoźny, Mateusz de Mezer, Anna Markowska, Janina Markowska, Jakub Żurawski","doi":"10.14670/HH-18-884","DOIUrl":"https://doi.org/10.14670/HH-18-884","url":null,"abstract":"Objective: We aimed to compare MLH1 and MSH2 protein expression and Mismatch repair (MMR) status with clinical data: diagnosis, grading, and staging. Moreover, we wanted to assess any correlation between two immunohistochemical assessments: classic microscopic and computer analysis performed by a calibrated program.Materials and methods: Our studies were conducted on 95 cases of endometrial cancer. For each, we performed H+E staining and immunohistochemistry (IHC) of two MMR status proteins: MLH1 and MSH2. Two independent researchers assessed IHC on a 0 to ++++ scale. We classified cases as MMR-deficient based on the microscopic assessment of the absence of expression of at least one protein. For computer analysis, we used Olympus cellSens software to measure the positive IHC reaction area in µm2 in five fields of vision.Results: Despite using two different assessment methods, we did not identify any statistically significant relationship between diagnosis, grading, staging, MMR protein expression, and MMR status. However, we found a strong correlation between computer analysis and semi-quantitative microscopic assessment (r=0.59 for MLH1 and r=0.76 for MSH2; p<0.001 for both). Furthermore, we revealed that computer measurement of the expression area could be a good objective prediction test for microscopic analysis (p<0.001).Conclusion: We did not find a relationship between MMR status and grading, staging, or diagnosis. However, we present a novel approach to immunohistochemical assessment using computer analysis. It allows us to carry out more objective and accurate studies with the IHC method.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18884"},"PeriodicalIF":2.5,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rosmarinic acid inhibits the proliferation of ovarian carcinoma cells by activating the p53/BAX signaling pathway. 迷迭香酸通过激活p53/BAX信号通路抑制卵巢癌细胞增殖。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-02-05 DOI: 10.14670/HH-18-883

İlhan Özdemır, Dilek Doğan Baş, Şamil Öztürk, Özge Karaosmanoğlu, Mehmet Cudi Tuncer

{"title":"Rosmarinic acid inhibits the proliferation of ovarian carcinoma cells by activating the p53/BAX signaling pathway.","authors":"İlhan Özdemır, Dilek Doğan Baş, Şamil Öztürk, Özge Karaosmanoğlu, Mehmet Cudi Tuncer","doi":"10.14670/HH-18-883","DOIUrl":"https://doi.org/10.14670/HH-18-883","url":null,"abstract":"Objective: While chemotherapeutic agents stop the development of cancer cells, they also kill healthy cells. This study aimed to increase anticancer effects and reduce side effects by combining a phytotherapeutic compound with a chemotherapeutic drug.Methods: This study examined the effects of nine concentrations of rosmarinic acid (RA) and doxorubicin (DOX) on human ovarian adenocarcinoma (OVCAR3) and skin keratinocyte (HaCaT) cell lines. Their cytotoxic effects were assessed based on cell viability, evaluated using the MTT assay, and apoptotic activity, evaluated using NucBlue staining and the gene and protein expression of tumor protein p53 (TP53) and BCL2 associated X, apoptosis regulator (BAX) quantified by qRT-PCR and western blots, respectively.Results: The half-maximal inhibitory concentration after 48 hours was 880.4 μM for RA and 2.26 μM for DOX. The cytotoxicity analysis revealed that cell viability decreased with the RA concentration. RA increased apoptosis in OVCAR3 cells by activating the p53/BAX pathway. Western blots showed that RA and DOX upregulated p53 and BAX protein levels in OVCAR3 cells.Conclusions: The RA and DOX combination inhibited cell proliferation by inducing apoptosis in OVCAR3 cells. These results suggest that RA may reduce the side effects of DOX toxicity.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18883"},"PeriodicalIF":2.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143407355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing acute pancreatitis: A novel method combining live cell imaging with tissue damage evaluation. 评估急性胰腺炎：一种结合活细胞成像和组织损伤评估的新方法。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-02-04 DOI: 10.14670/HH-18-882

Polona Kovačič, Maša Skelin Klemen, Eva Paradiž Leitgeb, Viktória Venglovecz, Lóránd Kiss, Gabriella Mihalekné Fűr, Andraž Stožer, Jurij Dolenšek

{"title":"Assessing acute pancreatitis: A novel method combining live cell imaging with tissue damage evaluation.","authors":"Polona Kovačič, Maša Skelin Klemen, Eva Paradiž Leitgeb, Viktória Venglovecz, Lóránd Kiss, Gabriella Mihalekné Fűr, Andraž Stožer, Jurij Dolenšek","doi":"10.14670/HH-18-882","DOIUrl":"https://doi.org/10.14670/HH-18-882","url":null,"abstract":"Acute pancreatitis (AP) is a sudden inflammation of the exocrine part of the pancreas, resulting in self-digestion and destruction of exocrine tissue. The intricate relationship between exocrine and endocrine functions is pivotal, as damage to acinar cells can affect endocrine cell function and vice versa. However, our understanding of these interactions remains limited. An effective strategy for investigating pancreatic cells involves the utilization of live in-situ acute mouse pancreas tissue slice preparations, combined with noninvasive fluorescent calcium labeling of endocrine or exocrine cells, and subsequent analysis using confocal laser scanning microscopy. Nevertheless, this approach encounters inherent conflicts with conventional methodologies employed to histologically assess the severity of tissue damage due to AP in the model. Traditional methods involve fixing and staining tissue samples with hematoxylin and eosin, thereby precluding live-cell imaging. In this study, our objective was to introduce an innovative method utilizing a commercial fluorescence Live/Dead assay that enables calcium imaging and tissue damage assessment in the same sample. This approach was validated against the classical histological grading of AP severity, and we found a good correlation between the classical histological grading method and the in-situ approach employing the Live/Dead assay. The primary advantage of our novel approach lies in its capacity to enable timely and efficient live-cell imaging together with damage assessment in the same tissue, thereby enabling the study of functional consequences of structural damage at the cellular level and reducing the number of animals required for experimentation.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18882"},"PeriodicalIF":2.5,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human equilibrative nucleoside transporter 1 and concentrative nucleoside transporter 1 in colorectal cancer: What do we know? A systematic review. 人类平衡核苷转运蛋白1和浓缩核苷转运蛋白1在结直肠癌中的作用：我们知道什么？系统回顾。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-02-03 DOI: 10.14670/HH-18-881

Matthew McKenna, Saranya Linganathan, Amber Li, Fiona Ruge, Jane Lane, Lin Ye, Wen Jiang, Rachel Hargest

{"title":"Human equilibrative nucleoside transporter 1 and concentrative nucleoside transporter 1 in colorectal cancer: What do we know? A systematic review.","authors":"Matthew McKenna, Saranya Linganathan, Amber Li, Fiona Ruge, Jane Lane, Lin Ye, Wen Jiang, Rachel Hargest","doi":"10.14670/HH-18-881","DOIUrl":"https://doi.org/10.14670/HH-18-881","url":null,"abstract":"Colorectal cancer (CRC) remains a major global health challenge despite advances in screening, diagnosis, and treatment. This systematic review examines the roles of Human Equilibrative Nucleoside Transporter 1 (hENT1) and Human Concentrative Nucleoside Transporter 1 (hCNT1) in CRC, focusing on their expression, regulation, and impact on chemotherapeutic efficacy, particularly with nucleoside analogues like 5-fluorouracil (5-FU). We conducted a comprehensive literature search following PRISMA guidelines, yielding 29 studies that met our inclusion criteria. The review reveals variable expression of hENT1 and hCNT1 in CRC tissues compared with normal tissues, with implications for treatment response and development of resistance. Increased hENT1 expression is associated with poor outcomes and resistance to 5-FU, suggesting its potential as a biomarker for predicting treatment response. Conversely, hCNT1's role appears more complex, with its expression influencing the efficacy of other chemotherapeutic agents like gemcitabine and capecitabine. The review also highlights the lack of robust, standardised methods for assessing mRNA and protein levels, which complicates the interpretation of data and the establishment of these transporters as reliable clinical markers. Key findings include the potential therapeutic benefits of modulating hENT1 and hCNT1 expression to enhance drug efficacy and overcome resistance. The study underscores the need for further research using standardised and advanced methodologies, such as 3D cell culture assays, to better understand the mechanistic pathways and clinical implications of nucleoside transporter expression in CRC. Future research should aim to clarify the roles of hENT1 and hCNT1 in CRC and chemoresistance to develop targeted therapies and improve patient outcomes.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18881"},"PeriodicalIF":2.5,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GATA4 is diagnostically useful for distinguishing primary ovarian mucinous carcinomas from metastatic colorectal adenocarcinomas to the ovary. GATA4 在诊断上有助于区分原发性卵巢粘液癌和转移到卵巢的结直肠腺癌。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-02-01 Epub Date: 2024-06-18 DOI: 10.14670/HH-18-783

Kunio Mochizuki, Ippei Tahara, Tomohiro Inoue, Kazunari Kasai, Tetsuo Kondo

{"title":"GATA4 is diagnostically useful for distinguishing primary ovarian mucinous carcinomas from metastatic colorectal adenocarcinomas to the ovary.","authors":"Kunio Mochizuki, Ippei Tahara, Tomohiro Inoue, Kazunari Kasai, Tetsuo Kondo","doi":"10.14670/HH-18-783","DOIUrl":"10.14670/HH-18-783","url":null,"abstract":"Aim: Determining the primary origin of an ovarian mucin-producing carcinoma can be challenging at times because some metastases of primary colorectal origin may exhibit gross, microscopic, and/or immunohistochemical features that overlap with those of primary ovarian mucinous carcinomas (OMCs). We hypothesized that GATA binding protein 4 (GATA4) might be a novel, useful marker for differentiating primary OMCs from metastatic colorectal adenocarcinomas to the ovary.Methodology: For comparison with the usefulness of other markers (special AT-rich sequence-binding protein 2 (SATB2) and caudal type homeobox 2 (CDX2)), we elucidated the expression profiles of GATA4 in OMCs, colorectal non-mucinous adenocarcinomas (CNMACs), and colorectal mucinous adenocarcinomas (CMACs) using immunohistochemistry.Results: We confirmed GATA4 expression (H-score ≥50 points) in 93%, SATB2 in 0%, and CDX2 in 64% of 14 OMCs. GATA4 was expressed in 13%, SATB2 in 90%, and CDX2 in 93% of 30 CNMACs. GATA4 was expressed in 20%, SATB2 in 73%, and CDX2 in 100% of 30 CMACs.Conclusion: The expression of GATA4 in a mucus-producing ovarian tumor strongly supports it being a primary OMC rather than a metastatic colorectal carcinoma: GATA4 expression indicates OMC and SATB2 expression indicates colorectal adenocarcinoma. However, three cases of colorectal adenocarcinoma were GATA4-positive and SATB2-negative, so the GATA4/SATB2 marker combination is not absolute for determining the primary site. Further research for more markers is necessary to find the ideal combination.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"183-189"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aromadendrin alleviates LPS-induced kidney apoptosis and inflammation by inhibiting phosphorylation of MAPK and NF-κB signaling pathways. 香树精通过抑制 MAPK 和 NF-κB 信号通路的磷酸化，缓解 LPS 诱导的肾脏凋亡和炎症。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-02-01 Epub Date: 2024-05-30 DOI: 10.14670/HH-18-770

Xiaohong Ma, Wenhua Liu, Bin Wang, Feizhuang Shi

{"title":"Aromadendrin alleviates LPS-induced kidney apoptosis and inflammation by inhibiting phosphorylation of MAPK and NF-κB signaling pathways.","authors":"Xiaohong Ma, Wenhua Liu, Bin Wang, Feizhuang Shi","doi":"10.14670/HH-18-770","DOIUrl":"10.14670/HH-18-770","url":null,"abstract":"Background: Excessive inflammation and apoptosis in kidneys are critical players in the pathogenesis of acute kidney injury (AKI). Aromadendrin is a natural flavonoid characterized by anti-inflammatory, anti-apoptotic, and antioxidant actions. Thus, we investigated the roles and mechanisms of aromadendrin in the development of AKI.Methods: Lipopolysaccharide (LPS) was used to induce AKI mice, and one hour after LPS challenge, the mice received oral administration of aromadendrin or vehicle. Renal functions were assessed by measuring blood urea nitrogen and creatinine in serum. Histological changes were determined by hematoxylin and eosin staining. Apoptotic cells of renal tissues were detected by TUNEL staining. Gene expression was measured by western blotting and RT-qPCR.Results: Aromadendrin alleviated LPS-induced renal dysfunctions and histological defects in mice. Additionally, aromadendrin suppressed excessive inflammation and tissue apoptosis in the kidneys of LPS-induced AKI mice. Mechanistically, aromadendrin blocked the activation of NF-κB and MAPK pathways in LPS-induced AKI mice.Conclusion: Aromadendrin alleviates LPS-stimulated inflammation and tissue cell apoptosis in kidneys by inactivating the NF-κB and MAPK pathways.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"249-258"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141317011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential expression of HIF-1α and its hypoxia-related inducers in the spleens of plateau yaks and plain yellow cattle. 高原牦牛和平原黄牛脾脏中 HIF-1α 及其缺氧相关诱导因子的差异表达。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-02-01 Epub Date: 2024-05-28 DOI: 10.14670/HH-18-768

Manlin Zhou, Shihui Dong, Jun Wang, Xuehui Luo, Rui Li, Yiyang Zhang, Haie Ding, Xiao Tan, Zilin Qiao, Kun Yang, Weiji Chen

{"title":"Differential expression of HIF-1α and its hypoxia-related inducers in the spleens of plateau yaks and plain yellow cattle.","authors":"Manlin Zhou, Shihui Dong, Jun Wang, Xuehui Luo, Rui Li, Yiyang Zhang, Haie Ding, Xiao Tan, Zilin Qiao, Kun Yang, Weiji Chen","doi":"10.14670/HH-18-768","DOIUrl":"10.14670/HH-18-768","url":null,"abstract":"The present study aims to investigate the distribution and expression characteristics of HIF-1α, VEGF, VEGFR-2, VCAM-1, and IL-4 in the spleen of plateau yaks and plain yellow cattle and to speculate the possible regulatory role of HIF-1α and its related hypoxia-inducible factors in the adaptation of the yak spleen to the plateau hypoxic environment. Histological features were observed using H&E and PAS stains. Immunohistochemical staining and optical density analysis were applied to investigate the distribution and differences in the expression of HIF-1α, VEGF, VEGFR-2, VCAM-1, and IL-4 in the spleen of yaks and cattle. The results showed that the area of splenic trabeculae and splenic nodules was significantly larger in the yak than in yellow cattle (P<0.05). Glycogen was mainly distributed in splenic arterial endothelial cells, vascular smooth muscle cells, splenic blood sinusoidal endothelial cells, and fibroblasts, and the distribution was significantly higher in the spleen of yaks than in cattle (P<0.05). HIF-1α, VEGF, VEGFR-2, VCAM-1, and IL-4 were mainly expressed in lymphocytes, arterial endothelial cells, vascular smooth muscle cells, splenic blood sinusoidal endothelial cells, and fibroblast cytoplasm, with higher expression in yak spleen (P<0.05). In conclusion, combining the differences in spleen tissue structure, glycogen distribution, and expression distribution of several hypoxia-related factors between yaks and cattle, we suggest that HIF-1α, VEGF, VEGFR-2, VCAM-1, and IL-4 may be important factors in the adaptation of yak spleen to the plateau environment, which provides a theoretical basis for further exploring the adaptation mechanism of plateau hypoxia in yaks.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"225-235"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lymph node or lymphoid aggregate? Impact on cancer resection quality, clinical prognosis, and tumor staging. 淋巴结还是淋巴集合体？对癌症切除质量、临床预后和肿瘤分期的影响。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-02-01 Epub Date: 2024-05-09 DOI: 10.14670/HH-18-760

Andreas Gocht, Christian Watermann, Madelaine Melzer, Udo Schumacher

引用次数: 0

TROP2 regulates cisplatin sensitivity of triple-negative breast cancer cells by regulating endoplasmic reticulum stress. TROP2通过调节内质网应激调节三阴性乳腺癌细胞对顺铂的敏感性。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-02-01 Epub Date: 2024-05-31 DOI: 10.14670/HH-18-771

Mingqi Zhang, Jianzhong Xu, Qing Liu, Xi Yan, Ning Li

{"title":"TROP2 regulates cisplatin sensitivity of triple-negative breast cancer cells by regulating endoplasmic reticulum stress.","authors":"Mingqi Zhang, Jianzhong Xu, Qing Liu, Xi Yan, Ning Li","doi":"10.14670/HH-18-771","DOIUrl":"10.14670/HH-18-771","url":null,"abstract":"Triple-negative breast cancer (TNBC) is a kind of breast cancer with a high metastasis rate and poor prognosis. As a transmembrane glycoprotein, tumor-associated calcium signal transducer 2 (TROP2) plays a certain role in the cancers. This study aimed to explore the potential mechanism of TROP2 affecting cisplatin (CDDP) resistance in TNBC from endoplasmic reticulum stress (ERS). MDA-MB-231 and CDDP-resistant cell lines MDA-MB-231/CDDP were used in this study, and the expression of TROP2 was detected by western blotting. After transfecting with the interference sequence of siRNA targeting TROP2, cell proliferation and apoptosis were detected by the cell counting kit-8, colony formation, and flow cytometry, and the expression of ERS-marker proteins was detected by western blotting. Furthermore, the effects of ERS in TROP2 on drug resistance of TNBC cells were explored by using ERS inhibitor 4-phenylbutyric acid (4-PBA). Results found that TROP2 expression in MDA-MB-231/CDDP was significantly upregulated compared with MDA-MB-231. The expression of TROP2 in MDA-MB-231/CDDP was significantly decreased after transfection with siRNA-TROP2, and the proliferation of MDA-MB-231 and MDA-MB-231/CDDP cells was significantly decreased after further induction with CDDP. TROP2 significantly affected TNBC cell cloning, apoptosis, and the expression of ERS-related marker proteins, while 4-PBA reversed the promoting effects of siRNA-TROP2 on apoptosis and ERS, as well as the inhibitory effects on cell proliferation, suggesting that TROP2 affected the resistance of TNBC cells to CDDP through ERS. In conclusion, TROP2 inhibited apoptosis of TNBC cells, improved the cell cloning ability, and regulated the sensitivity of TNBC cells to CDDP through ERS.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"259-268"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glucan-rich polysaccharides obtained from split gill mushroom [Schizophyllum commune (Fr.)] ameliorate hyperglycemia by enhancing insulin and GLUT2 pancreas in type 2 diabetic rats. 从裂鳃蘑菇[Schizophyllum commune (Fr.)] 中提取的富含葡聚糖的多糖可通过增强胰岛素和 GLUT2 胰腺来改善 2 型糖尿病大鼠的高血糖症状。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-02-01 Epub Date: 2024-06-24 DOI: 10.14670/HH-18-784

Udomlak Matsathit, Decha Sermwittayawong, Manaras Komolkriengkrai, Wipapan Khimmaktong

{"title":"Glucan-rich polysaccharides obtained from split gill mushroom [Schizophyllum commune (Fr.)] ameliorate hyperglycemia by enhancing insulin and GLUT2 pancreas in type 2 diabetic rats.","authors":"Udomlak Matsathit, Decha Sermwittayawong, Manaras Komolkriengkrai, Wipapan Khimmaktong","doi":"10.14670/HH-18-784","DOIUrl":"10.14670/HH-18-784","url":null,"abstract":"Aims: Abnormalities in the secretion of insulin are the cause of pathology and complications in diabetic patients. The aim of this study was to investigate the anti-diabetic effect of polysaccharide extracts from the split gill mushroom in type 2 diabetes rats administered a low dose of streptozotocin (STZ) in combination with a high-fat diet.Methods: The rats were divided into 6 groups: the control group (ND), the control group fed with polysaccharide extract from split gill (ND240), the diabetes group (HFD+DM), the diabetic group fed 120 (HFD+S120) and 240 mg/kg BW polysaccharide extract (HFD+S240), and the diabetic group receiving metformin (HFD+Met). Subsequently, the Islets of Langerhans of pancreatic tissue were studied using a light microscope and transmission electron microscopy (TEM). Immunofluorescence for the detection of insulin and glucose transporter 2 (GLUT2) proteins, and malondialdehyde (MDA) were also detected in pancreatic tissue.Results: In the diabetic and HFD+120 groups, the tissues harbored various pathologies. The HFD+S240 and HFD+Met groups were found to have lower blood sugar levels. The levels of insulin and GLUT2 increased compared with the diabetic group. Additionally, the levels of MDA were reduced.Conclusions: The use of polysaccharide extract from split gill mushrooms (240 mg/kg BW) is an alternative to treating various pathologies in the relief or treatment of diabetes mellitus.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"191-203"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0