Histology and histopathology最新文献

The Lian-Dou-Qing-Mai Formula activates the PPARγ-LXRα-ABCA1/ABCG1 pathway by regulating IL-10, leading to the promotion of cholesterol efflux and a reduction in atherosclerotic plaques. 连豆青麦方通过调节 IL-10 激活 PPARγ-LXRα-ABCA1/ABCG1 通路，从而促进胆固醇外流，减少动脉粥样硬化斑块。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-04-01 Epub Date: 2024-08-19 DOI: 10.14670/HH-18-803

Wenqi Liao, You Li, Haoyan Zhao, Shu Lu

{"title":"The Lian-Dou-Qing-Mai Formula activates the PPARγ-LXRα-ABCA1/ABCG1 pathway by regulating IL-10, leading to the promotion of cholesterol efflux and a reduction in atherosclerotic plaques.","authors":"Wenqi Liao, You Li, Haoyan Zhao, Shu Lu","doi":"10.14670/HH-18-803","DOIUrl":"10.14670/HH-18-803","url":null,"abstract":"Background: To observe the effect of the Lian-Dou-Qing-Mai (LDQM) formula on lipid metabolism in mice and explore its mechanism from the perspective of regulating the PPARγ/LXRα/ABCA1 signaling pathway.Methods: THP-1 cells were induced to transform into foam cells with ox-LDL. Atherosclerosis (AS) models were constructed using a high-fat diet in ApoE-/- mice. Detection kits were used to evaluate triglyceride (TG) and total cholesterol (TC) content; TNF-α, MCP-1, MMP-9, TMP-1, PPARγ, LXRα, ABCA1, and ABCG1 mRNA and protein expression were identified using real-time PCR and western blot. Aortic plaque development and lipid deposition were seen using hematoxylin and eosin (HE) and oil red O staining, respectively.Results: In the cell model, LDQM could inhibit the formation of THP-1 macrophage-derived foam cells and the expression of inflammatory factors, promote macrophage cholesterol efflux, increase the expression of IL-10, and activate the PPARγ-LXRα-ABCA1/ABCG1 pathway. Additional IL-10 treatment further promotes LDQM-induced cholesterol efflux in THP-1 cells; In vivo models, LDQM inhibited the area of atherosclerotic lesions, aortic lipid deposition, and inflammation levels in ApoE-/- mice through IL-10, and activated the expression level of the PPARγ-LXRα-ABCA1/ABCG1 pathway.Conclusion: LDQM may affect the PPARγ/LXRα/ABCA1 signaling pathway through IL-10, regulate lipid metabolism, reduce serum inflammatory expression and lipid deposition, and improve the formation of atheroplaques.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"585-596"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of 6-OHDA injection and microtrauma in the rat substantia nigra on local brain amyloid beta protein concentrations in the affected area. 大鼠黑质注射 6-OHDA 和微创伤对受累区域脑淀粉样 beta 蛋白浓度的影响

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-04-01 Epub Date: 2024-10-22 DOI: 10.14670/HH-18-836

Joshua A Roldán-Kalil, Sara E Vendrell-Gonzalez, Natalia Espinosa-Ponce, Jadier Colón-Vasques, Jescelica Ortiz-Rivera, Vassiliy Tsytsarev, Janaina M Alves, Mikhail Inyushin

{"title":"Impact of 6-OHDA injection and microtrauma in the rat substantia nigra on local brain amyloid beta protein concentrations in the affected area.","authors":"Joshua A Roldán-Kalil, Sara E Vendrell-Gonzalez, Natalia Espinosa-Ponce, Jadier Colón-Vasques, Jescelica Ortiz-Rivera, Vassiliy Tsytsarev, Janaina M Alves, Mikhail Inyushin","doi":"10.14670/HH-18-836","DOIUrl":"10.14670/HH-18-836","url":null,"abstract":"Amyloid beta peptides (Aβ) are key indicators of Alzheimer's disease and are also linked to cognitive decline in Parkinson's disease (PD) and other neurodegenerative disorders. This study explored the accumulation of Aβ in a standard 6-Hydroxydopamine (6-OHDA) model of PD. We unilaterally injected 6-OHDA into the substantia nigra of Wistar rats to induce dopaminergic cell degeneration and death, a characteristic of PD. The goal was to detect Aβ protein in tissues and blood vessels showing inflammation or degeneration from the 6-OHDA injection. Our results showed that 6-OHDA injection produced a statistically significant rise in Aβ concentration at the injection site 60 minutes after injection, which was slightly reduced 24 hours post-injection but still significantly higher than in controls. We also tried Gp120 injection in the same zone but it only produced effects comparable to control needle trauma. The presence of Aβ in tissues and blood vessel walls after injection was confirmed through ELISA tests and was supported by immunohistochemical staining of injection areas. We found that the increased Aβ concentration was visible in and around blood vessels and inside blood vessel walls, and also, to a lesser extent in some cells, most probably neurons, in the area. This research highlights the connection between dopaminergic cell poisoning and the accumulation of Aβ, offering insights into the progression of PD to cognitive disorders and dementia.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"485-492"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological features and genetic background in ectomesenchymal chondromyxoid tumor: A systematic review. 外胚层软骨瘤的形态特征和遗传背景：系统综述。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-04-01 Epub Date: 2024-09-05 DOI: 10.14670/HH-18-808

Raquel Helena Junia de Souza, Fernanda Aragão Felix, Flávia Martins Vasconcelos Filiú, Witalo Pereira de Jesus, Felipe Paiva Fonseca, Ricardo Santiago Gomez, Lucas Guimarães Abreu, Sílvia Ferreira de Sousa

{"title":"Morphological features and genetic background in ectomesenchymal chondromyxoid tumor: A systematic review.","authors":"Raquel Helena Junia de Souza, Fernanda Aragão Felix, Flávia Martins Vasconcelos Filiú, Witalo Pereira de Jesus, Felipe Paiva Fonseca, Ricardo Santiago Gomez, Lucas Guimarães Abreu, Sílvia Ferreira de Sousa","doi":"10.14670/HH-18-808","DOIUrl":"10.14670/HH-18-808","url":null,"abstract":"Background: Ectomesenchymal chondromyxoid tumor (EMCMT) is a rare neoplasm that mainly affects the tongue and harbors recurrent, although not exclusive, gene fusions. Owing to its rarity, overlapping features with other tumors may lead to challenges in the microscopic diagnosis. We aimed to perform a systematic review focusing on the histomolecular findings of EMCMT of the oral and maxillofacial region and to evaluate the possible association between microscopic features with the genetic background.Methods: An electronic search was made on PubMed, Web of Science, Scopus, Ovid, and Embase. Clinicopathological, immunohistochemical, and molecular data were retrieved.Results: Overall, 114 cases from 53 articles on EMCMT were analyzed. Histologically, EMCMT was described as demarcated (84.2%), lobulated (66.7%), reticulated (51.8%), and arranged in sheets, cords, and strands (42.9%), with 73.7% of lesions with spindle-shaped cells. Myxoid stroma (88.6%), chondroid areas (60.5%), chondromyxoid stroma (57.0%), and fibrous septae (42.9%) were also tumor-outlined features. The most expressed markers were vimentin (100.0%), cyclin D1 (100.0%), GFAP (88.5%), NSE (87.5%), S100 (86.5%), CD56 (76.9%), and CD57 (76.5%). The RREB1-MRTFB fusion was detected in 91.0% of the cases investigated and EWSR1 rearrangements in 17.4%. The presence of the fusion RREB1::MRTFB or chromosome alterations in the EWSR1 gene were not highly specific to the morphological features of EMCMT.Conclusion: This study provides a comprehensive summary of the clinicopathological, immunohistochemical, and molecular characteristics of EMCMT, aiding in a more accurate microscopic diagnosis of this rare tumor.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"431-441"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SIRT4 is associated with microvascular infiltration, immune cell infiltration, and epithelial mesenchymal transition in hepatocellular carcinoma. SIRT4 与肝细胞癌的微血管浸润、免疫细胞浸润和上皮间质转化有关。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-04-01 Epub Date: 2024-07-12 DOI: 10.14670/HH-18-794

Juan Li, Ming Zhao, Weiwei Fan, Na Na, Hui Chen, Ming Liang, Sheng Tai, Shan Yu

{"title":"SIRT4 is associated with microvascular infiltration, immune cell infiltration, and epithelial mesenchymal transition in hepatocellular carcinoma.","authors":"Juan Li, Ming Zhao, Weiwei Fan, Na Na, Hui Chen, Ming Liang, Sheng Tai, Shan Yu","doi":"10.14670/HH-18-794","DOIUrl":"10.14670/HH-18-794","url":null,"abstract":"Aims: Hepatocellular carcinoma (HCC) is the third leading cause of cancer death worldwide. In the present study, we evaluated SIRT4 expression levels in HCC specimens and investigated the relationships between SIRT4 expression levels, clinicopathological factors, and microvascular infiltration (MVI) in HCC.Methods: The expression levels of SIRT4 in 108 HCC specimens were examined by immunohistochemical staining. MVI in HCC specimens was divided into three subtypes: M0, M1, and M2. Comprehensive bioinformatics analysis was carried out to demonstrate SIRT4's biological functions and expression-related prognostic value.Results: The diffuse cytoplasmic expression pattern of SIRT4 was observed in all adjacent nonneoplastic liver tissues. The levels of SIRT4 were higher in HCC than in any other type of cancer and normal tissues. In addition, the expression levels of SIRT4 were significantly decreased in HCC tissues when MVI was M1 or M2 (p=0.003) but were not related to the overall clinical outcome. To explain MVI regulated by SIRT4, we also found that SIRT4 expression correlated with epithelial-mesenchymal transition (EMT) markers and CD4+ T/NK cells and downregulated cancer-associated fibroblast cells. Also, there was a significant relationship between MVI and degree of cell differentiation (p=0.003), tumor size (p<0.001), alpha fetoprotein (AFP) (p=0.001), alanine aminotransferase (ALT) (p=0.024), and γ-glutamyl transferase (γ-GT) (p=0.024). However, SIRT4 was not an independent prognostic marker of HCC.Conclusions: Our results demonstrated an association between SIRT4 expression levels, MVI, immune cell infiltration, and potential biological functions, including EMT in the progression of HCC.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"523-540"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression feature and prognostic function of a novel immune checkpoint Siglec-15 in human colorectal cancer. 新型免疫检查点 Siglec-15 在人类结直肠癌中的表达特征和预后功能。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-04-01 Epub Date: 2024-07-30 DOI: 10.14670/HH-18-801

Guodong Liang, Linyun Zhou, Yang Fan, Run Ding, Junrong Yang, Li Xu, Yidan Zhu, Wen Huang

{"title":"Expression feature and prognostic function of a novel immune checkpoint Siglec-15 in human colorectal cancer.","authors":"Guodong Liang, Linyun Zhou, Yang Fan, Run Ding, Junrong Yang, Li Xu, Yidan Zhu, Wen Huang","doi":"10.14670/HH-18-801","DOIUrl":"10.14670/HH-18-801","url":null,"abstract":"Background: Sialic acid-bound immunoglobulin lectin 15 (Siglec-15) plays an important role in the development of cancer. However, the association between Siglec-15 expression and clinicopathological characteristics of colorectal cancer (CRC) has not been fully investigated.Methods: In this present study, a number of bioinformatics analyses were performed to provide an overview and detailed characteristics of Siglec-15. Quantitative real-time polymerase chain reaction (qPCR), western blotting and immunohistochemistry analyses were conducted to characterize the expression of Siglec-15 in CRC. Kaplan-Meier survival and Cox regression analyses were performed to identify the prognostic parameters of CRC.Results: The results of bioinformatics analyses revealed the expression characteristics and prognostic roles of Siglec-15 in CRC. The data of qCPR, western blotting, and IHC analyses demonstrated that the expression of Siglec-15 in CRC tissues was significantly higher than that in non-cancerous tissues. Moreover, the expression level of Siglec-15 in CRC was significantly associated with lymph node metastasis (p=0.001), TNM stage (p=0.001), and overall survival (p=0.026). COX multi-factor analysis indicated that Siglec-15 expression (p=0.023) and tumor differentiation (p=0.003) were independent prognostic factors for CRC.Conclusions: Collectively, the data suggested that Siglec-15 expression may serve as a novel prognostic factor and Siglec-15 might be identified as an ideal candidate for immunotherapy in CRC treatment.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"571-584"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of transfection on inflammatory factor production in LPS-induced HBE cells. 转染对 LPS 诱导的 HBE 细胞中炎性因子产生的影响。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-04-01 Epub Date: 2024-07-18 DOI: 10.14670/HH-18-798

Bo Zhang, Jie Cao

{"title":"Effects of transfection on inflammatory factor production in LPS-induced HBE cells.","authors":"Bo Zhang, Jie Cao","doi":"10.14670/HH-18-798","DOIUrl":"10.14670/HH-18-798","url":null,"abstract":"Transfection is an experimental technique typically used in biological experiments. In this study, we verified whether this technique may cause the release of inflammatory cytokines and affect cell viability. We used lipopolysaccharide (LPS)-induced human bronchial epithelial (16-HBE) cells as a model to evaluate whether cell transfection with Lipofectamine 3000 would affect LPS-induced inflammatory factors in HBE cells. MicroRNA (miRNA) negative control (NC)- and miR-584-mimics were transfected into 16-HBE cell lines. The 584-mimic was used to increase the expression of miR-584, and the NC-mimic was used to add a negative control sequence. After 24h of transfection, the cells were incubated with LPS for another 24h, and the effects on the release of inflammatory cytokines, such as IL-1, IL-6, IL-8, TNF-α, MIP-1, MCP-1α, and cell viability were investigated. The optimal conditions for transfection were evaluated, and cytokine and chemokine mRNA levels were determined. Regardless of the NC- or 584-mimic, the results indicate that the expression of transfected genes in these cells leads to an increase in inflammatory factors and decreased cell viability. Microscope analysis revealed that the number of HBE cells was lower after transfection, and many small vesicles could be observed in the transfected cells, indicating that the insertion of gene vectors may affect the biological activity of HBE cells and experimental results. Results suggest that Lipofectamine 3000 transfected miRNA into HBE cells, providing better transfection rates, however, at the cost of higher toxicity.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"541-545"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and molecular aspects of lung development. 肺发育的形态学和分子方面。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-04-01 Epub Date: 2024-09-04 DOI: 10.14670/HH-18-807

Andreas Schmiedl, Christian Mühlfeld

引用次数: 0

Baicalin promotes migration and angiogenesis of endothelial progenitor cells but impedes thrombus formation via SIRT1/NF-κB signaling in a rat model of deep vein thrombosis. 在大鼠深静脉血栓形成模型中，黄芩苷可促进内皮祖细胞的迁移和血管生成，但通过SIRT1/NF-κB信号转导阻碍血栓形成。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-04-01 Epub Date: 2024-07-26 DOI: 10.14670/HH-18-799

Jinfeng Xie, Yonggui Liao, Dile Wang

{"title":"Baicalin promotes migration and angiogenesis of endothelial progenitor cells but impedes thrombus formation via SIRT1/NF-κB signaling in a rat model of deep vein thrombosis.","authors":"Jinfeng Xie, Yonggui Liao, Dile Wang","doi":"10.14670/HH-18-799","DOIUrl":"10.14670/HH-18-799","url":null,"abstract":"Background: Deep vein thrombosis (DVT) is the third most prevalent vascular disease worldwide, seriously threatening human health. Baicalin, a flavonoid isolated from the roots of Scutellaria baicalensis, has been identified to play a crucial role in various vascular diseases. The study aimed to explore the efficacy and underlying mechanisms of baicalin in DVT.Methods: Endothelial progenitor cells (EPCs) were differentiated from peripheral blood mononuclear cells isolated from rat bone marrow. Dil-ac-LDL/FITC-UEA-1 double staining and flow cytometry analysis were conducted for the identification of EPCs. The angiogenesis and migration of EPCs in vitro were tested by a tube formation assay and Transwell assay, respectively. DVT rat models were established by stenosis of the inferior vena cava (IVC). After the euthanasia of rats, thrombi in the IVC were collected and weighed, and histological alterations in IVC tissue were measured by H&E staining. The protein levels of SIRT1, p-P65, and p65 in rat IVC tissues were quantified via western blotting.Results: EPCs used in this study displayed a spindle-like shape and were positive for endothelial cell-specific markers, suggesting the phenotypic characteristics of EPCs. Baicalin enhanced the migratory and angiogenetic abilities of EPCs in vitro. For in vivo experiments, baicalin reduced thrombus weight and mitigated DVT formation in model rats. Moreover, baicalin activated SIRT but repressed NF-κB signaling in IVC tissues of DVT rats.Conclusion: Baicalin facilitates migration and angiogenesis of EPCs but impedes thrombus formation via regulation of SIRT1/NF-κB signaling in DVT model rats.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"547-554"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collagen fiber arrangement in the normal bladder lamina propria and their potential impact on the pathological substaging of bladder cancer stage T1. 正常膀胱固有层中的胶原纤维排列及其对膀胱癌 T1 期病理分期的潜在影响。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-04-01 Epub Date: 2024-09-19 DOI: 10.14670/HH-18-817

Ofer N Gofrit, Vladimir Yutkin, Mordechai Duvdevani, Guy Hidas, Tzahi Neuman

{"title":"Collagen fiber arrangement in the normal bladder lamina propria and their potential impact on the pathological substaging of bladder cancer stage T1.","authors":"Ofer N Gofrit, Vladimir Yutkin, Mordechai Duvdevani, Guy Hidas, Tzahi Neuman","doi":"10.14670/HH-18-817","DOIUrl":"10.14670/HH-18-817","url":null,"abstract":"The lamina propria (LP) of the urinary bladder lies between the urothelial mucosa and the muscularis propria. This complex stratum is composed of extracellular matrix, several cell types, and collagen types I and III fibers. LP invasion by urothelial carcinoma (progression from stage Ta to T1) is a determinant of bladder cancer advancement. We attempted to characterize collagen fiber arrangement in the LP. This could enrich our understanding of this important layer and potentially provide clues for sub-staging of the T1 bladder cancer. A total of 24 Masson trichrome-stained images of normal bladder, including 12,530 collagen fibers were quantitatively analyzed using the Dragonfly software. The LP was divided according to fiber orientation into superficial LP (SLP, 15% of the thickness) and the deep LP (DLP, 85% of the thickness). Collagen fiber geometry analysis demonstrated that the SLP fibers are more parallel to the urothelium with an average angle of 260±230 compared to 400±260 in the DLP (p=3.4x10-144), more packed (average distance to the closest fiber of 0.61±0.67 compared to 0.66±0.77, p=0.0001), and their aspect ratio is considerably longer (average of 1.93±0.12 compared to 0.20±0.11, p=2.84x10-8). No difference was found in fiber perimeter or Feret diameter. Thus, we conclude that bladder collagen fibers are arranged in two distinct layers: a dense-ordered SLP and a loose disorder DLP. This indicates that the physical barrier to cancer cell invasion probably lies in the SLP, immediately underneath the urothelium. Once this barrier is breached, the looser and disorganized DLP poses no remarkable obstacle. Thus, we believe that histology-based subdivisions of stage T1 are expected to fail in providing clinically meaningful prognostic information.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"477-483"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Golgi method. A historical through contemporary view. 高尔基方法。从历史到当代的视角。

IF 2.5 4区生物学

Histology and histopathology Pub Date : 2025-04-01 Epub Date: 2024-09-25 DOI: 10.14670/HH-18-821

Ignacio González-Burgos

{"title":"The Golgi method. A historical through contemporary view.","authors":"Ignacio González-Burgos","doi":"10.14670/HH-18-821","DOIUrl":"10.14670/HH-18-821","url":null,"abstract":"Knowledge regarding the biology of the nervous system and its functions has gone through various theoretical, methodological, and interpretative stages throughout history, depending largely on technical advances that have allowed us not only to approach old questions from new perspectives but also to address new ones. One advance that constituted a watershed in the history of neuroscience was the appearance of a chrome-silver staining technique called the Golgi method that allowed the complete, three-dimensional observation of nerve cells. Discovered by Camilo Golgi and, later, modified significantly and employed by Santiago Ramón y Cajal, Golgi's method was crucial in demonstrating the veracity of the Neuronal Theory over the earlier Reticular Theory, and in revealing numerous findings related to the human brain and those of many other animal species, which continue to be analyzed today. Despite a period of scientific recession in the first half of the 20th century, the use of the Golgi method prevailed and even expanded in the second half of that century and into the 21st, as researchers continued to use it in its original or modified form and in combination with emerging methodologies. Currently, there are no signs of any decline in its use.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"453-465"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0