Histology and histopathology最新文献_第3页

Role of connexin 43 in odontogenesis and odontogenic tumors. 连接蛋白43在牙形成和牙源性肿瘤中的作用。

IF 2 4区生物学

Histology and histopathology Pub Date : 2025-10-07 DOI: 10.14670/HH-25-002

Ronell Bologna-Molina

{"title":"Role of connexin 43 in odontogenesis and odontogenic tumors.","authors":"Ronell Bologna-Molina","doi":"10.14670/HH-25-002","DOIUrl":"https://doi.org/10.14670/HH-25-002","url":null,"abstract":"Connexin 43 (Cx43) is a transmembrane protein forming gap junctions essential for intercellular communication, regulating ion and molecule exchange, and coordinating key developmental and pathological processes. In odontogenesis, Cx43 expression in ameloblasts and odontoblasts orchestrates differentiation, mineralization, and tissue repair. Its dynamic regulation influences enamel and dentin formation, while altered expression is linked to defective tooth development. Beyond dental tissues, Cx43 participates in craniofacial morphogenesis and bone remodeling. In odontogenic tumors, Cx43 shows heterogeneous expression patterns, reflecting its role in tumor architecture, differentiation, and aggressiveness. High mesenchymal expression is seen in ameloblastic fibromas and fibro-odontomas, whereas follicular ameloblastomas and odontogenic keratocysts exhibit downregulation, particularly in basal cells-correlating with increased proliferation, anti-apoptosis, and autophagy markers. These variations in odontogenic tumors suggest that Cx43 may act as a tumor suppressor by maintaining epithelial organization and regulating cell polarity. Molecularly, Cx43 interacts with MAPK/ERK, PI3K/AKT, and Wnt/β-catenin pathways, influencing cell cycle control, apoptosis, and epithelial-mesenchymal interactions. Loss of Cx43 disrupts gap junctional intercellular communication, potentially enhancing tumor progression. Therapeutically, modulating Cx43 could inhibit tumor angiogenesis, restore normal growth control, and promote differentiation toward less aggressive phenotypes. However, given its dual role in tumor suppression and tissue repair, targeted interventions must be context-specific. Cx43 emerges as a promising diagnostic, prognostic, and therapeutic biomarker in different neoplasias, warranting further investigation to optimize clinical applications. The objective of this work is to review current information on the role of CX43 in normal tissue and odontogenic tumors to evaluate its possible usefulness as a future therapeutic target.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"25002"},"PeriodicalIF":2.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elp3 activates the JNK/MAPK pathway through histone acetylation to promote gastric cancer proliferation, migration, and invasion. Elp3通过组蛋白乙酰化激活JNK/MAPK通路，促进胃癌的增殖、迁移和侵袭。

IF 2 4区生物学

Histology and histopathology Pub Date : 2025-10-06 DOI: 10.14670/HH-25-001

Ji Di, Hao Shang, Xiali Shi, Hanmei Jiang

{"title":"Elp3 activates the JNK/MAPK pathway through histone acetylation to promote gastric cancer proliferation, migration, and invasion.","authors":"Ji Di, Hao Shang, Xiali Shi, Hanmei Jiang","doi":"10.14670/HH-25-001","DOIUrl":"https://doi.org/10.14670/HH-25-001","url":null,"abstract":"Background: Gastric cancer (GC) seriously affects the life and health of patients, and the role of Elp3 in GC is still unclear; therefore, the aim of this study was to investigate the role and mechanism of Elp3 overexpression in GC.Methods: Elp3-overexpressing HGC27 cells were constructed with an overexpression plasmid, and Elp3-overexpressing GC nude mice were prepared, which were intervened by histone acetylation inhibitor (SAHA) or JNK pathway inhibitor (SP600125). The protein interactions between Elp3 and JNK1 were verified by Co-immunoprecipitation (Co-IP) assay. Cell proliferation, migration, invasion, JNK/MAPK pathway, and histopathological changes were evaluated with CCK-8, clone formation assay, scratch assay, Transwell, qRT-PCR, western blot, and HE staining.Results: Elp3 interacted with JNK1 protein, and Elp3 overexpression promoted GC proliferation, invasion, migration, elevated HAT activity, and activation of the JNK/MAPK pathway. A histone acetylation inhibitor attenuated the promotional effect of Elp3 overexpression on GC and activation of the JNK/MAPK pathway. Further, inhibition of the JNK/MAPK pathway also suppressed the promotion of GC by Elp3 overexpression.Conclusion: Elp3 may be involved in GC progression by activating the JNK/MAPK pathway through histone acetylation.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"25001"},"PeriodicalIF":2.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparable expression of stem markers and damage-responsive proteins in untreated and chemoradiation-treated rectal cancer. 未治疗和放化疗的直肠癌中干细胞标志物和损伤反应蛋白的表达比较。

IF 2 4区生物学

Histology and histopathology Pub Date : 2025-10-03 DOI: 10.14670/HH-18-999

Kentaro Tsuji, Sachi Sekine, Hirotoshi Kawata, Tomoko Kamiakito, Takeo Nakaya, Yasuyuki Miyakura, Koichi Suzuki, Toshiki Rikiyama, Akira Tanaka

{"title":"Comparable expression of stem markers and damage-responsive proteins in untreated and chemoradiation-treated rectal cancer.","authors":"Kentaro Tsuji, Sachi Sekine, Hirotoshi Kawata, Tomoko Kamiakito, Takeo Nakaya, Yasuyuki Miyakura, Koichi Suzuki, Toshiki Rikiyama, Akira Tanaka","doi":"10.14670/HH-18-999","DOIUrl":"https://doi.org/10.14670/HH-18-999","url":null,"abstract":"Purpose: Cancer stem cells (CSCs) are suggested to contribute to therapy resistance in rectal cancer. However, histopathological analysis of CSCs is still lacking in rectal cancer.Methods: The expression of leucine-rich, repeat-containing G protein-coupled receptor 5 (LGR5), proto-oncogene, polycomb ring finger 1 (BMI1), yes-associated transcriptional regulator (YAP) and its paralog TAZ (hereafter, YAP/TAZ), and nuclear β-catenin was compared in untreated and chemoradiation-treated (CRT) rectal cancer by in situ hybridization and immunostaining. Niche factors were also compared in human rectal cancer specimens and the embryonic intestine.Results: The mean ratios were 15% and 14% for LGR5, 30% and 33% for BMI1, 2.7% and 7.6% for YAP/TAZ, and 38% and 32% for nuclear β-catenin in untreated and CRT rectal cancer, respectively, showing no significant differences for these stem markers following CRT. The stromal expression ratios of YAP/TAZ were 9.7% and 23% in untreated and CRT rectal cancer, which indicated significant upregulation and confirmation of the damage response in the stroma of CRT tumors. In addition, cancer cells co-expressing LGR5 and CDKN1B are also comparable in untreated and CRT rectal cancer. For niche factors, we found that WNT2B and GREM1 were uniformly expressed in rectal cancer with a pattern similar to that of the early embryonic intestine.Conclusion: The expression of LGR5, BMI1, CDKN1B, and YAP/TAZ was comparable in untreated and CRT rectal cancer. The uniform expression of mesenchymal niche factors might prevent the zonation of the stem cell niche in rectal cancer.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18999"},"PeriodicalIF":2.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145212327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alteration in epithelium and stroma of Post-INTACS cornea: Ultrastructure and 3D transmission electron tomography. intacs后角膜上皮和基质的改变：超微结构和3D透射电子断层扫描。

IF 2 4区生物学

Histology and histopathology Pub Date : 2025-10-02 DOI: 10.14670/HH-18-998

Saeed Akhtar, Omar Kirat, Adrian Smedowski, Aljohara Alkanaan, Fahad Almoqbel, Turki Almubrad

{"title":"Alteration in epithelium and stroma of Post-INTACS cornea: Ultrastructure and 3D transmission electron tomography.","authors":"Saeed Akhtar, Omar Kirat, Adrian Smedowski, Aljohara Alkanaan, Fahad Almoqbel, Turki Almubrad","doi":"10.14670/HH-18-998","DOIUrl":"https://doi.org/10.14670/HH-18-998","url":null,"abstract":"This study was conducted to investigate the ultrastructure of the epithelium and stroma of post-INTACS corneas. INTACS were surgically inserted into the corneas of three patients to remodel the keratoconus shape. INTACS were inserted with the IntraLase femtosecond laser. Patients 1, 2, and 3 returned to the clinic 8, 6, and 9 years, respectively, after surgery due to their deteriorating vision. The lamellae above the INTACS were removed surgically and processed for light and electron microscopy. 2D and 3D digital images of lamellae, collagen fibrils (CFs), and proteoglycans (PGs) were captured by a bottom-mounted camera and analysed using iTEM software. The epithelium and stromal lamellae had degenerated. A large number of aggregates of microfilaments were present in the Bowman's layer (BW) and throughout the stroma. In the stroma, just above the INTACS insertion, lamellae were completely disorganised and running randomly in the large electron-lucent spaces. The CF diameter was significantly smaller than in the normal cornea. 3D images showed microfibrils within the CF were less in the post-INTACS cornea than in the normal cornea. We believe that insertion of the INTACS disturbed the lamellar organisation and uniform distribution of CFs. This non-uniform CF distribution increased over time, resulting in vision impairment.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18998"},"PeriodicalIF":2.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The prognostic value of adipokine receptors leptinR and adiponectinR1 in obesity-inducible solid tumours. 脂肪因子受体瘦素r和脂联素r1在肥胖诱导实体瘤中的预后价值。

IF 2 4区生物学

Histology and histopathology Pub Date : 2025-10-01 Epub Date: 2025-02-27 DOI: 10.14670/HH-18-895

Isabella Bollen, Marit Bernhardt, Thore Thiesler, Franziska Isabelle Winterhagen, Andreas Türler, Manuel Ritter, Alexander Mustea, Glen Kristiansen

{"title":"The prognostic value of adipokine receptors leptinR and adiponectinR1 in obesity-inducible solid tumours.","authors":"Isabella Bollen, Marit Bernhardt, Thore Thiesler, Franziska Isabelle Winterhagen, Andreas Türler, Manuel Ritter, Alexander Mustea, Glen Kristiansen","doi":"10.14670/HH-18-895","DOIUrl":"10.14670/HH-18-895","url":null,"abstract":"Background: With the rising incidence of life expectancy, obesity, and tumours, understanding the incretory influence of adipose tissue in tumorigenesis becomes increasingly important. As the adipokines leptin and adiponectin are released by fat tissue, we aimed to analyse the expression of their respective receptors in tumours for which an association with obesity is epidemiologically hypothesised.Methods: The expression of leptinR and adipoR1 were analysed in cohorts of renal cell cancer (n=391), cervical cancer (n=155), vulvar cancer (n=107), and endometrial cancer (n=90) by immunohistochemistry and correlated with clinicopathological parameters including survival times.Results: Expression of leptinR was high in renal cell cancer (62.2%), vulvar carcinoma (50%), and endometrial cancer (80.5%). High expression was associated with favourable clinicopathological markers and longer overall survival times in renal cell and vulvar cancer. AdipoR1 was only weakly expressed in all four tumour entities and did not show significant associations with clinicopathological parameters or prognosis.Conclusion: High leptinR is a promising biomarker of favourable tumour outcomes in renal cell carcinoma and vulvar carcinoma.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"1577-1596"},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advantages and limitations of 3,3',5,5'-tetramethylbenzidine for immunohistochemical staining. 3,3',5,5'-四甲基联苯胺免疫组化染色的优点与局限性。

IF 2 4区生物学

Histology and histopathology Pub Date : 2025-10-01 Epub Date: 2025-02-18 DOI: 10.14670/HH-18-888

Chao Yu, Xiao Liu, Peiyuan Zhao, Zhidong Sun, Yurong Song, Yuan Cao, Ming Cheng

{"title":"Advantages and limitations of 3,3',5,5'-tetramethylbenzidine for immunohistochemical staining.","authors":"Chao Yu, Xiao Liu, Peiyuan Zhao, Zhidong Sun, Yurong Song, Yuan Cao, Ming Cheng","doi":"10.14670/HH-18-888","DOIUrl":"10.14670/HH-18-888","url":null,"abstract":"In this study, two chromogenic systems, horseradish peroxidase (HRP)-3,3'-diaminobenzidine (DAB) and HRP-3,3',5,5'-tetramethylbenzidine (TMB), were used to perform single-color and double-color immunohistochemical staining (sIHC and dIHC, respectively) on multiple antigens in four distinct tissue types. The chromogenic results of the HRP-TMB system exhibited a vibrant blue-green color, and the tissue localization and signal intensity were consistent with those of the HRP-DAB system. In addition, it demonstrated clear differentiation from the hematoxylin-stained nucleus, endogenous melanin, and brown chromogenic results of HRP-DAB. TMB staining in tissues that contain high endogenous pigment levels eliminates the need for melanin bleaching, thereby facilitating direct observation and potentially improving the detection speed and interpretation. TMB can also be used in combination with DAB for dIHC, thus allowing detection of the two markers on a single slide. However, the TMB staining results are not stable over the long term and require image storage using slide scanners, thereby limiting its application. Additionally, in dIHC, overlapping signals of the first marker may obscure the second marker, potentially leading to bias or false negatives. Therefore, careful consideration is required when designing dIHC detection systems. Based on the above, we propose that TMB is a valuable supplement to DAB for immunohistochemical staining and deserves further promotion and utilization. However, additional research is needed to improve the composition of TMB chromogenic reagents, prolong the longevity of staining results, and overcome current limitations.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"1667-1674"},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress of research on engineered extracellular vesicles from different sources for disease treatment. 不同来源的工程化细胞外囊泡治疗疾病的研究进展。

IF 2 4区生物学

Histology and histopathology Pub Date : 2025-10-01 Epub Date: 2025-03-12 DOI: 10.14670/HH-18-903

Jinhui Kang, Jun Wen, Huifang Chen, Cui Zhu, Yinshan Bai

引用次数: 0

Zengye decoction regulated the expression of aquaporin in colon tissue of rats with constipation through miR-10a-5p targeting Drd2/AC/cAMP axis. 增液汤通过靶向Drd2/AC/cAMP轴的miR-10a-5p调控便秘大鼠结肠组织水通道蛋白的表达。

IF 2 4区生物学

Histology and histopathology Pub Date : 2025-10-01 Epub Date: 2025-01-21 DOI: 10.14670/HH-18-876

Simin Chen, Qian Li, Lina Guan, Jun Pang, Qingfeng Wu, Qiuxiao Wang, Zhibin Zhang, Xuegui Tang

{"title":"Zengye decoction regulated the expression of aquaporin in colon tissue of rats with constipation through miR-10a-5p targeting Drd2/AC/cAMP axis.","authors":"Simin Chen, Qian Li, Lina Guan, Jun Pang, Qingfeng Wu, Qiuxiao Wang, Zhibin Zhang, Xuegui Tang","doi":"10.14670/HH-18-876","DOIUrl":"10.14670/HH-18-876","url":null,"abstract":"Slow transit constipation (STC) is a colonic motor disorder characterized by a marked delay in the movement of substances through the colon. Traditional Chinese medicine (TCM) is a treasure trove of natural compounds, which is effective in treating constipation with relatively minor side effects. Zengye decoction (ZYD), a classic herbal formula in TCM, is used for moistening the intestines and relieving constipation. However, the molecular mechanism of ZYD in the treatment of constipation remains unclear. The present study aimed to investigate the effect and molecular mechanism of ZYD on STC. A loperamide-induced rat model and IEC-18 cells were used in vitro and in vivo. We found that ZYD increased the intestinal transit rate and fecal water content of STC rats in a dose-dependent manner. Furthermore, ZYD dose-dependently decreased the expression of Aquaporin (AQP)3 and increased AQP9 expression in the colon tissue of STC rats. Mechanistically, ZYD reduced the level of miR-10a-5p, increased the expression of dopamine receptor D2 (Drd2), and inhibited the activity of adenylate cyclase (AC)/cyclic adenosine monophosphate (cAMP) signaling in the colon tissue of STC rats. However, miR-10a-5p overexpression reversed the improvement effect of ZYD on constipation. Moreover, miR-10a-5p targeted Drd2 and enhanced AC/cAMP signaling activation in IEC-18 cells. Also, miR-10a-5p regulated AQP3 and AQP9 expression in IEC-18 cells. Thus, ZYD alleviated constipation and aquaporin expression in STC rats, and its mechanism may be mediated by downregulating miR-10a-5p levels and inhibiting the Drd2/AC/cAMP axis. ZYD may be an efficient therapeutic strategy for constipation.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"1617-1630"},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppressive role of fukutin on cell proliferation in uterine cervical carcinoma in relation to Aurora-A kinase. 福库汀对子宫颈癌细胞增殖的抑制作用与 Aurora-A 激酶的关系

IF 2 4区生物学

Histology and histopathology Pub Date : 2025-10-01 Epub Date: 2025-02-19 DOI: 10.14670/HH-18-889

Tomoko Yamamoto, Yukinori Okamura, Yoichiro Kato, Kenta Masui, Yoji Nagasima, Atsushi Kurata

{"title":"Suppressive role of fukutin on cell proliferation in uterine cervical carcinoma in relation to Aurora-A kinase.","authors":"Tomoko Yamamoto, Yukinori Okamura, Yoichiro Kato, Kenta Masui, Yoji Nagasima, Atsushi Kurata","doi":"10.14670/HH-18-889","DOIUrl":"10.14670/HH-18-889","url":null,"abstract":"Fukutin, the gene responsible for Fukuyama congenital muscular dystrophy (FCMD), is involved in the glycosylation of α-dystroglycan (α-DG). On the other hand, fukutin is expressed in various organs, and the roles of fukutin in non-neuromuscular tissues are not fully elucidated. In the present immunohistochemical study of uterine cervical carcinoma, Ki-67-positive cells tended to be more in areas showing weaker expression of fukutin. In HeLa cells, Ki-67-positive cells were increased after the suppression of fukutin by RNAi, and were decreased by overexpression of fukutin. Similar results were obtained upon phosphorylation of histone H3 at serine 10, which is enriched during mitosis. Interestingly, Aurora-A kinase (AURKA), one of the proteins regulating Ser10 phosphorylation of histone H3, was highly expressed in HeLa cells, and the phosphorylation of AURKA was reduced by overexpression of fukutin. Furthermore, fukutin is co-localized with targeting protein for Xklp2 (TPX2), a protein enhancing AURKA activity. Fukutin may be able to suppress cell proliferation by reducing AURKA phosphorylation, probably competing with TPX2.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"1555-1568"},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Galangin alleviates gastric mucosal injury in rats with chronic atrophic gastritis by reducing ferroptosis. 高良姜对慢性萎缩性胃炎大鼠胃黏膜损伤的减轻作用是通过减轻铁下垂。

IF 2 4区生物学

Histology and histopathology Pub Date : 2025-10-01 Epub Date: 2025-01-24 DOI: 10.14670/HH-18-878

Tian Yang, Min Lu, Weiqiang Jiang, Dandan Jin, Meiling Sun, Hua Mao, Huixia Han

{"title":"Galangin alleviates gastric mucosal injury in rats with chronic atrophic gastritis by reducing ferroptosis.","authors":"Tian Yang, Min Lu, Weiqiang Jiang, Dandan Jin, Meiling Sun, Hua Mao, Huixia Han","doi":"10.14670/HH-18-878","DOIUrl":"10.14670/HH-18-878","url":null,"abstract":"Objective: Chronic atrophic gastritis (CAG) is a precancerous lesion and is the first stage in a multistep precancerous cascade that can lead to gastric adenocarcinoma. This study aimed to reveal the role and mechanism of galangin in CAG.Methods: Rats were intragastrically administered a mixture of 2% sodium salicylate and 30% alcohol, forced to exercise, and fasted irregularly to establish CAG models. To explore the efficacy of galangin on CAG rats, we used Hericium erinaceus (HE) and omeprazole (Ome) as controls. The degree of gastric mucosal injury was assessed by H&E staining and immunohistochemistry. Perls staining and western blot analysis were used to assess iron content and enrichment of ferroptosis-related proteins. Reactive oxygen species and mitochondrial superoxide in the mucosa were visualized by probes. The morphology of cells was examined by transmission electron microscopy.Results: Our data showed that galangin treatment alleviated gastric mucosal damage and reduced ferroptosis in CAG rats, manifested as decreased iron content, iron transporters and storage proteins, decreased ROS and mitochondrial superoxide, and partially restored cellular morphology. Of note, galangin at a high concentration had better treatment efficacy than HE but lower than Ome.Conclusions: This study demonstrated that galangin reduced gastric mucosal injury in CAG rats by inhibiting ferroptosis. These findings provide a theoretical basis for its clinical application and broaden its potential applications.","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"1631-1638"},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0