Histology and histopathology最新文献

{"title":"Exosomal miR-143-3p released from cancer cells suppresses cervical cancer progression and promotes ferroptosis through directly targeting KLF5.","authors":"Cunyan Qian, Xia Cai, Lei Shi, Ailing Li, Weiwei You, Dongmei Liu, Yuan Xie","doi":"10.14670/HH-25-146","DOIUrl":"https://doi.org/10.14670/HH-25-146","url":null,"abstract":"<p><p>Cervical cancer is the most prevalent gynecological malignancy. Tumor-derived exosomal microRNAs (miRNAs) have been increasingly considered important players in cervical cancer; however, the specific function and molecular mechanism of exosomal miR-143-3p in cervical cancer are unclear. MiR-143-3p and Kruppel-like factor 5 (KLF5) expression were detected using CancerMIRNome, UALCAN, RT-qPCR, and immunoblotting. The cargo information of miR-143-3p was obtained in ExoCarta, and cervical cancer cell-derived exosomes were identified and characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and immunoblotting. CCK-8 and EDU assays were used to detect cell proliferation, while wound healing and Transwell assays were used to detect cell migration and invasion, respectively. TargetScan and luciferase reporter assays were used to predict and test the interaction between miR-143-3p and KLF5. Assay kits were used to measure glutathione (GSH), malondialdehyde (MDA), and reactive oxygen species (ROS) levels. Tumor growth, metastasis, and cervical damage were detected in cervical cancer-bearing mice. Here, miR-143-3p was lowly expressed in cervical cancer tissues and cells and was highly enriched in cervical cancer-derived exosomes. MiR-143-3p mimic inhibited proliferation, migration, and invasion, and induced cell-cycle arrest of cervical cancer cells. MiR-143-3p targeted and downregulated KLF5. KLF5 was overexpressed in cervical cancer, and its silencing suppressed the proliferation, migration, and invasion of cervical cancer cells. Moreover, KLF5 deficiency drove ferroptosis; KLF5 overexpression reversed the impacts of miR-143-3p on tumor growth and metastasis in cervical cancer both <i>in vitro</i> and <i>in vivo</i>. Our work underscored that miR-143-3p, carried and transferred by cervical cancer cell-derived exosomes, plays an anti-oncogenic role in cervical cancer via KLF5.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"25146"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149109000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of heat-reinforcing Needling on the PPAR-γ/NF-κB signaling pathway in the synovial membrane of rats with rheumatoid arthritis.","authors":"Hongtao Zhang, Chaozhan Ren, Guangjun Zhang, Xinghua Zhang","doi":"10.14670/HH-25-067","DOIUrl":"10.14670/HH-25-067","url":null,"abstract":"<p><p>The study seeks to examine how heat-reinforcing needling (HRN) affects the PPAR-γ/NF-κB signaling pathway in synovial membranes of rats with rheumatoid arthritis (RA). Forty-eight male Sprague-Dawley rats were randomly assigned to four groups (control, model, rosiglitazone, and HRN groups). RA was induced using bovine type II collagen and Freund's adjuvant in cold and moist conditions. The rats in the rosiglitazone group were given an intraperitoneal injection of 10 mg/kg rosiglitazone, whereas those in the acupuncture group received treatment at the Zusanli acupoint. Following the 14-day intervention, synovial tissue was stained with H&amp;E to examine pathological alterations and blood levels of TNF-α, IL-6, IL-1β, and IL-4. The expressions of PPAR-γ and NF-κB in synovial tissue were determined by Western blot analysis and real-time PCR. The model group exhibited higher fibrous tissue growth and inflammatory cell infiltration in comparison with the control group, but serum IL-4 and PPAR-γ expressions were inhibited (<i>p</i><0.01), and TNF-α, IL-6, IL-1β, and NF-κB expression levels in the serum were elevated (<i>p</i><0.01). In comparison with the model group, the HRN and rosiglitazone groups showed lower serum levels of TNF-α, IL-6, IL-1β, and NF-κB and higher levels of serum IL-4 and PPAR-γ (<i>p</i><0.05). In the RA cold syndrome model rats, HRN may reduce joint swelling and suppress inflammation of the synovial membrane, possibly via controlling the PPAR-γ/NF-κB signaling pathway. This method could provide a fresh therapeutic approach to RA symptom management.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"1709-1717"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147511744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining gelatin methacryloyl hydrogels with mesenchymal stem cells: A versatile approach for bone tissue engineering.","authors":"Juefei Wu, Yuhui Sun, Wenting Zhang, Tianyi Li, Jiaqi Zan, Jiayi Zhu, Xiaoling Ding, Gang Ding","doi":"10.14670/HH-25-066","DOIUrl":"10.14670/HH-25-066","url":null,"abstract":"<p><p>Bone tissue engineering (BTE) represents an innovative strategy for repairing bone defects by integrating biomaterials, cells, and bioactive factors, aiming to mimic the structural and functional properties of natural bone tissue to facilitate regeneration. Bone healing is a multi-stage process involving inflammation, soft callus formation, hard callus development, and bone remodeling, which are regulated by the synergistic action of various cells and molecular signaling pathways. Gelatin methacryloyl (GelMA) hydrogel scaffolds, with their excellent biocompatibility, tunable mechanical properties, and photocrosslinking ability, provide a three-dimensional (3D) microenvironment similar to the natural extracellular matrix for cells. In recent years, combining mesenchymal stem cells (MSCs) with GelMA hydrogels has become a cutting-edge strategy for bone regeneration. GelMA not only supports the survival and proliferation of MSCs but also promotes osteogenic differentiation and accelerates bone defect repair through its adjustable physicochemical properties. This review, based on current literature, explores the unique properties of MSCs and the latest advancements in the synergistic effects of MSCs and GelMA hydrogels in promoting bone regeneration.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"1647-1660"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147511680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RRM2/GCH1 signaling decreased cisplatin sensitivity in cervical cancer by modulating the ferroptosis process.","authors":"Jin Zhao, Linlin Wang, Ning Shao, Yaoyu Qu, Wei Chen, Huizhen Sun","doi":"10.14670/HH-25-056","DOIUrl":"10.14670/HH-25-056","url":null,"abstract":"<p><strong>Background: </strong>Cisplatin-based chemotherapy serves as a fundamental approach in the treatment of cervical cancer; however, its effectiveness is often hindered by the development of drug resistance, which presents a significant obstacle in clinical practice.</p><p><strong>Methods: </strong>Differentially expressed genes (DEGs) related to ferroptosis in cervical cancer were examined compared to healthy controls, using data from the Gene Expression Omnibus (GEO) database and the ferroptosis database (FerrDb V2). Lipid peroxidation, ferrous ion (Fe²⁺) concentration, and reactive oxygen species (ROS) were examined. <i>In vitro</i> and <i>in vivo</i> studies were carried out to explore the influence of Ribonucleotide reductase M2 (RRM2) on the effectiveness of cisplatin chemotherapy.</p><p><strong>Results: </strong>We identified an aggregate of 4,385 statistically significant DEGs, among which 122 genes were related to ferroptosis. Moreover, the ferroptosis-related gene <i>RRM2</i> was notably overexpressed in cervical cancer and demonstrated remarkable sensitivity and specificity for diagnosing cervical cancer. Additionally, the expression levels were inversely correlated with the outcomes of patients with cervical cancer. Furthermore, <i>RRM2</i> silencing increased cervical cancer cell ferroptosis and enhanced the efficacy of cisplatin treatment <i>in vitro</i> and <i>in vivo</i>. Finally, our experiments established a positive association between RRM2 and the classical ferroptosis inhibition factor GTP cyclohydrolase 1 (GCH1) by immunofluorescence and immunoblotting.</p><p><strong>Conclusion: </strong>RRM2 plays roles in the evaluation and outcome prediction of cervical cancer, potentially impacting cisplatin sensitivity through regulation of ferroptosis. This investigation provides novel insights into the tumorigenic functions of RRM2, highlighting its potential use as a prognostic biomarker in cervical carcinoma.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"1681-1694"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147365021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of p53 and Smad4 immunohistochemistry in pancreatic ductal adenocarcinoma and validation by next-generation sequencing.","authors":"Ziqi Zhao, Juan Du, Wenyang Guo, Lingchao Liu, Congrong Liu, Limei Guo","doi":"10.14670/HH-25-072","DOIUrl":"10.14670/HH-25-072","url":null,"abstract":"<p><strong>Background: </strong>Mutations in four major driver genes -<i>KRAS, CDKN2A, TP53,</i> and <i>SMAD4</i>- are central to the pathogenesis of pancreatic ductal adenocarcinoma (PDAC) and critically inform diagnosis, therapeutic decision-making, and prognostic assessment. Although next-generation sequencing (NGS) is widely regarded as the gold standard for detecting these mutations, its clinical application is often limited by suboptimal analytical efficiency and substantial economic cost. Among these genes, immunohistochemical (IHC) staining for the proteins encoded by <i>TP53</i> and <i>SMAD4</i> has been extensively adopted in routine pathology practice. However, standardized IHC pattern classification schemes and rigorous validation of their predictive accuracy for underlying genomic alterations remain lacking in PDAC.</p><p><strong>Methods: </strong>We retrospectively enrolled 63 PDAC patients and systematically characterized the typical IHC expression patterns of p53 and Smad4. Targeted NGS was subsequently performed on all available tumor specimens, and the resulting mutational profiles were correlated with corresponding IHC findings. Diagnostic performance including sensitivity, specificity and accuracy of p53 IHC for predicting <i>TP53</i> mutations and of Smad4 IHC for predicting <i>SMAD4</i> mutations was rigorously evaluated.</p><p><strong>Results: </strong>Among the four canonical driver genes, co-occurring double- or triple-gene mutations were prevalent; within <i>TP53</i> and <i>SMAD4</i>, missense mutations constituted the most frequent variant type. Using NGS as the reference standard, we validated the diagnostic utility of a three-tiered p53 IHC classification system, particularly in fine-needle biopsy (FNB) specimens. Furthermore, we proposed a novel, refined Smad4 IHC pattern classification that incorporates an \"intermediate\" category, thereby expanding upon conventional binary interpretation. This new scheme achieved markedly improved mutation prediction accuracy (0.76) compared with traditional approaches (0.57).</p><p><strong>Conclusion: </strong>Our study highlights the complementary diagnostic value of p53 and Smad4 IHC relative to molecular testing in PDAC, especially when tissue is limited, as commonly encountered in FNB specimens. The newly established Smad4 IHC classification system, which integrates an intermediate expression category into the conventional two-tier framework, demonstrates superior clinical utility and enhances predictive accuracy for <i>SMAD4</i> genomic alterations.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"1743-1753"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147622803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methyltransferase 3 promotes v-set and transmembrane domain-containing 2-like protein expression to intensify ferroptosis-mediated prostate adenocarcinoma progression through the m6A methylation modification.","authors":"Jinming Li, Jun Jiang, Shaoxing Wang, Jiahao Liu, Shusen Zuo","doi":"10.14670/HH-25-070","DOIUrl":"10.14670/HH-25-070","url":null,"abstract":"<p><strong>Background: </strong>Prostate adenocarcinoma (PRAD) is a common malignancy with high incidence in men. The role of v-set and transmembrane domain-containing 2-like protein (VSTM2L) in PRAD remains largely unreported.</p><p><strong>Methods: </strong>Gene expression was analyzed using The Cancer Genome Atlas (TCGA), the Tumor Immune Estimation Resource (TIMER) 2.0, and the University of Alabama at Birmingham CANcer data analysis Portal (UALCAN) databases, and validated by quantitative real-time PCR (qRT-PCR) and western blot. Cell proliferation was assessed by 5-ethynyl-2'-deoxyuridine (EdU) staining. Apoptosis and mitochondrial membrane potential were examined by flow cytometry. Intracellular iron, Fe²⁺, and reactive oxygen species (ROS) levels were measured using commercial kits and flow cytometry. The role of VSTM2L in tumor growth was evaluated using xenograft mouse models, with protein expression in tumors evaluated by immunohistochemistry (IHC). The N6-methyladenosine (m6A) modification sites on VSTM2L mRNA were predicted using the sequence-based RNA adenosine methylation site predictor (SRAMP) website. The interaction between methyltransferase 3 (METTL3) and VSTM2L was confirmed by methylated RNA immunoprecipitation (MeRIP) and dual-luciferase reporter assay. Correlation analysis was performed using the TCGA database.</p><p><strong>Results: </strong>VSTM2L was overexpressed in PRAD tissues and cell lines. Silencing VSTM2L inhibited PRAD cell proliferation, promoted apoptosis, and enhanced ferroptosis and oxidative stress <i>in vitro</i>. Consistently, VSTM2L knockdown suppressed tumor growth <i>in vivo</i>. Mechanically, METTL3 mediated m6A methylation to stabilize VSTM2L mRNA. Furthermore, METTL3 promoted proliferation and inhibited apoptosis, ferroptosis, and oxidative stress in PRAD cells via a VSTM2L-dependent manner.</p><p><strong>Conclusion: </strong>METTL3 promotes PRAD progression by stabilizing VSTM2L expression through m6A methylation, thereby inhibiting ferroptosis. This study establishes a direct link between RNA methylation and ferroptosis in PRAD, revealing the METTL3/VSTM2L axis as a novel regulatory pathway and a potential therapeutic target.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"1731-1741"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological changes in ventricular false chordae tendineae in patients with dilated cardiomyopathy.","authors":"Yaping Xu, Chenlei Zhou, Yuexin Yu, Shuqi Zou, Zhikun Fu, Yan Dong, Bin Yang, Xiangli Zhang, Zhikun Guo","doi":"10.14670/HH-25-069","DOIUrl":"10.14670/HH-25-069","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the pathological changes in ventricular false chordae tendineae in patients with dilated cardiomyopathy (DCM).</p><p><strong>Methods: </strong>Gross anatomy, histology, immunohistochemistry, and immunofluorescence techniques were employed to examine the anatomical features, tissue composition, and the distribution of blood and lymphatic vessels in the false chordae tendineae of the left and right ventricles from six DCM patients and one normal heart. The expression and localization of the gap junction protein connexin 43 (Cx43) in the false chordae tendineae were also assessed.</p><p><strong>Results: </strong>False chordae tendineae were observed in both left and right ventricles, with a greater number found in the left ventricle. Based on histological characteristics observed with Masson's trichrome staining, false chordae tendineae were categorized into four types: muscular (cardiomyocytes), conductive (Purkinje fibers), mixed (both), and tendinous (dense connective tissue). The false chordae tendineae were surrounded by a thick connective tissue sheath, which extended inward to separate the internal cells into clusters or islands. Cx43 was abundantly expressed between cells, predominantly localized at the lateral-lateral regions, with fewer end-to-end connections. The interior of the false chordae tendineae was rich in blood and lymphatic vessels.</p><p><strong>Conclusion: </strong>In DCM patients, the gross morphology of ventricular false chordae tendineae showed no significant changes; however, marked proliferation of fibrous connective tissue was frequently observed, separating cardiomyocytes or Purkinje fibers into bundles or completely replacing the cellular components. This may contribute to the pathological basis of reentrant arrhythmias. Furthermore, the remodeling of Cx43 from an end-to-end to a lateral-lateral distribution may underlie electrical heterogeneity.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"1719-1730"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147573680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Telocytes in skeletal muscle: Emerging players in homeostasis and repair/regeneration.","authors":"Irene Rosa, Eloisa Romano, Mirko Manetti","doi":"10.14670/HH-25-071","DOIUrl":"10.14670/HH-25-071","url":null,"abstract":"<p><p>Telocytes (TCs) have recently emerged as novel components of the skeletal muscle interstitium. They are distinguished from other stromal cells by their immunophenotypic profiles and, especially, unique ultrastructural traits. Specifically, TCs feature a small cell body and very long, thin telopodes with a moniliform appearance conferred by the alternation of slender segments (podomers) and small dilated portions (podoms). Experimental evidence suggests that, as part of the skeletal muscle stem cell niche, TCs may be involved in orchestrating satellite cell activation and myogenic differentiation through both direct physical interactions and paracrine signaling. Yet, further in-depth research is needed to uncover specific immunophenotypic signatures for skeletal muscle TCs within the niche, as well as to identify the signaling pathways by which they influence neighboring satellite cells and, possibly, other cellular components of the niche. In the present review, particular emphasis is placed on the putative strategic role of TCs in maintaining skeletal muscle tissue homeostasis, their involvement in muscle pathological alterations, and, most importantly, their possible role in the coordination of the regenerative response following injury. In perspective, the promising therapeutic potential of TC-based strategies to enhance skeletal muscle tissue repair/regeneration and restrain post-injury fibrosis is also discussed.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"1635-1646"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147591947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The clinical significance and mechanism of DCIS-like morphology of lymph node metastasis in breast cancer.","authors":"Xin Lin, Mingmei Fan, Jingquan Liu, Ting Long, Lingfang Gao, Haibin Wu, Yan Zhong, Wenwen Lin, Ruian Wang, Zuguo Li, Meihua Chen","doi":"10.14670/HH-25-145","DOIUrl":"https://doi.org/10.14670/HH-25-145","url":null,"abstract":"<p><strong>Background: </strong>During the practice of diagnostic pathology, we identified a case of invasive ductal carcinoma (IDC) of the breast with lung metastases exhibiting a ductal carcinoma in situ (DCIS)-like morphology. Immunohistochemistry confirmed the presence of CK5/6, P40 and P63-positive tumor cells at the periphery of the metastatic nests. Subsequent expansion of our sample revealed similar DCIS-like morphology in lymph node metastases of other IDC cases. This study aimed to preliminarily characterize the pathological features, significance, and mechanisms of this phenomenon.</p><p><strong>Methods: </strong>We collected 101 lymph node metastasis specimens of breast IDC. (1) DCIS-like metastases were identified, and their correlation with clinicopathological parameters was analyzed. (2) Immunohistochemistry for basal/myoepithelial markers (CK5/6, P63, SMA, Calponin) was performed to assess their expression in relation to DCIS-like morphology. (3) Stemness markers (ALDH1A1, CD44, SOX2) were detected, and their expression was correlated with clinicopathological parameters, DCIS-like morphology, and basal/myoepithelial differentiation.</p><p><strong>Results: </strong>(1) DCIS-like morphology was observed in 24.8% (25/101) of lymph node metastases. Its presence correlated with higher nodal burden and non-luminal molecular subtypes (HER2-enriched and triple-negative). The recurrence rate of the DCIS-like metastasis group was higher than that of the non-DCIS-like metastasis group. (2) A subset of cases showed tumor cell expression of P63, CK5/6, or SMA in metastases, while none expressed Calponin. The proportion of cases expressing basal/myoepithelial markers was higher in the DCIS-like metastasis group, with a significant correlation between DCIS-like morphology and CK5/6 expression. (3) High stemness marker expression correlated significantly with higher histological grade, increased numbers of lymph node metastases, advanced pTNM stage, the presence of DCIS-like morphology, and basal/myoepithelial differentiation.</p><p><strong>Conclusion: </strong>The presence of DCIS-like morphology and basal/myoepithelial marker expression in lymph node metastases of breast IDC is associated with enhanced tumor stemness. DCIS-like metastases may serve as a valuable morphological indicator of higher tumor aggressiveness and increased recurrence risk.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"25145"},"PeriodicalIF":2.2,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149080486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High expression of glycoprotein non-metastatic melanoma protein B is associated with resistance to neoadjuvant chemotherapy in triple-negative breast cancer.","authors":"Xiaofan Xu, Yuanyuan Cao, Xueying Kong, Jingyi Wang, Ziwei Wu, Qiurong Li","doi":"10.14670/HH-25-144","DOIUrl":"https://doi.org/10.14670/HH-25-144","url":null,"abstract":"<p><strong>Aims: </strong>This retrospective single-center study aimed to investigate the expression of glycoprotein non-metastatic melanoma protein B (GPNMB) and its association with clinicopathological parameters, Miller-Payer (MP) grading, neoadjuvant chemotherapy (NAC), and prognosis in triple-negative breast cancer (TNBC).</p><p><strong>Methods: </strong>A total of 50 female patients with TNBC, who were subjected to NAC followed by surgery between January 2010 and March 2024 at Jinling Hospital (Nanjing Medical University, China), were included in this study. The formalin-fixed and paraffin-embedded (FFPE) core biopsy and excision samples of these patients were obtained from the pathology department. The FFPE samples were subjected to histological analysis using hematoxylin and eosin (H&amp;E) staining and immunohistochemistry (IHC) staining of the estrogen receptor, progesterone receptor, human epidermal growth factor receptor-2, proliferation marker Ki-67, and GPNMB. The response of excision FFPE samples to NAC was evaluated by H&amp;E staining with an MP grading system. Finally, the relationship between GPNMB expression and clinicopathological parameters, MP grade, and prognosis of patients with TNBC was statistically analyzed using univariable and multivariable logistic regression methods. Functional experiments of GPNMB in TNBC cells were carried out <i>in vitro</i>.</p><p><strong>Results: </strong>Positive expression of GPNMB was significantly associated with low MP grade and poor prognosis of patients with TNBC. Knockdown of GPNMB suppressed proliferation and migration, promoted apoptosis, and enhanced chemosensitivity in TNBC cells.</p><p><strong>Conclusions: </strong>These results indicate that GPNMB expression can serve as a potential biomarker for predicting the pathological response to NAC (in addition to MP grading) and as a prognostic marker for TNBC. The important limitation of this study is the small number of patients; further validation is needed.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"25144"},"PeriodicalIF":2.2,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149074594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}