Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University最新文献_第4页

[Clinical analysis of intrahepatic cholestasis during pregnancy in 150 patients]. 妊娠期肝内胆汁淤积150例临床分析

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Yi-ling Ding, Ling-ling Tan, Wen Deng

{"title":"[Clinical analysis of intrahepatic cholestasis during pregnancy in 150 patients].","authors":"Yi-ling Ding, Ling-ling Tan, Wen Deng","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To explore the effect of intrahepatic cholestasis of pregnancy (ICP) on the mother and fetus, and to assess the clinical value of nonstress test.Methods: The symptoms, biochemical examinations, fetal monitoring, and pregnant outcomes of 150 patients with ICP were analyzed retrospectively in our hospital.Results: There was no linear relation among serum cholanic acid, glutamic-pyruvic transaminase, total bilirubin, and gestational age when skin pruritus of pregnant women appeared, but there was positive correlation among the neonate status, meconium-stained amniotic fluid, and the onset time of pruritus. There was no correlation between the amount of postpartum hemorrhage and the newborn Apgar scores. No significant difference was found in perinatal prognosis The appearance of skin between normal and abnormal outcomes in the nonstress test.Conclusion: pruritus can be a sign of the state of ICP patients. To carefully monitor and comprehensively and continuously estimate the fetal state, and to terminate the pregnancy at proper time are very important to improve the prognosis of perinatal infants.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 6","pages":"645-7"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25035122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Eight cases of ovarian mature cystic teratoma with malignant transformation]. 卵巢成熟囊性畸胎瘤伴恶性转化8例。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Lan-qin Cao, Xin-guo Li, Xin Li

引用次数: 0

[Construction of double-subunit co-expression eukaryotic vector of human interleukin 12]. [人白细胞介素12双亚基共表达真核载体的构建]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Dong-ye Yang, Fang-gen Lu, Shui-ping Zhao

{"title":"[Construction of double-subunit co-expression eukaryotic vector of human interleukin 12].","authors":"Dong-ye Yang, Fang-gen Lu, Shui-ping Zhao","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To construct the double-subunit co-expression plasmid P(+)/IL-12 to fulfill the gene therapy.Methods: The full length cDNAs of P40 and P35, two subunits of hIL12, were amplified from the reverse transcription production of human embryo kidney using polymerase chain reaction (PCR) and respectively cloned into eukaryotic expression vector pcDNA3.1(+/-) to construct the single-subunit plasmids--P(+)/P40 and P(-)/P35. Then we linked cDNAs of P40 and P35 tandem and cloned them into pcDNA3.1(+) to get P(+)/IL-12 plasmid. The hIL-12 protein in supernate was detected with enzyme-linked immunoabsorbent assay (ELISA) after P(+)/IL-12 was transfected into HepG2 through liposome.Results: The plasmid of P(+)/IL-12 was testified using enzyme cutting and sequencing analysis. ELISA showed that hIL-12 protein could be expressed after transfecting into HepG2.Conclusion: The successful construction of P(+)/IL-12 plasmid can not only simplify the operation steps but also mimic hIL-12 physiological expression style in the gene therapy, thus laying the foundation for the use of hIL-12 in the gene therapy.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"338-42"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24104923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effects of lithium chloride on the proliferation and apoptosis of K562 leukemia cells]. [氯化锂对K562白血病细胞增殖和凋亡的影响]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Hua-rong Tang, Qun He

引用次数: 0

[Particle enhanced immunoturbidimetic assay of high sensitive C-reactive protein]. [颗粒增强免疫比浊法测定高敏感c反应蛋白]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Min Hu, Yan-wu Li, Yong-hong Hu

引用次数: 0

[Hysterosalpingography combined with hysteroscopy-laporoscopy in diagnosing female infertility]. [子宫输卵管造影联合宫腔镜-腹腔镜诊断女性不孕症]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Xian-ming Luo, Yi-ming Yang, Qiao Shi

引用次数: 0

[Inhibitive effect of soybean isoflavone on prostate hyperplasia in rats]. [大豆异黄酮对大鼠前列腺增生的抑制作用]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Guo-feng Ren, Yi-ming Huang

引用次数: 0

[Serum nitric oxide and D-dimer before and after administering antihypertensive drugs in essential hypertension]. [原发性高血压患者服用降压药前后血清一氧化氮和d -二聚体的变化]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Wei-ru Zhang, Ming Sun, Jie-kun Luo

{"title":"[Serum nitric oxide and D-dimer before and after administering antihypertensive drugs in essential hypertension].","authors":"Wei-ru Zhang, Ming Sun, Jie-kun Luo","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the relationship among serum nitric oxide, D-dimer, blood pressure, dangerous states and target organ damage in essential hypertension and to evaluate the effects of enalapril and terazosin.Methods: Fifty-five patients were randomized into the enalapril group and terazosin group, each group receiving enalapril and terazosin respectively for 8 weeks. Serum nitric oxide and D-dimer were measured before and after the treatment.Results: Serum nitric oxide was lower (P < 0.001) but D-dimer was higher (P < 0.01) in the hypertensions than those in the controls. Serum nitric oxide and D-dimer were related to different levels of blood pressure, dangerous states and target organ damage; Both enalapril and terazosin could reduce the level of blood pressure; Both could increase the serum nitric oxide (P < 0.001) and decrease the D-dimer(P < 0.01).Conclusion: Endothelial dysfunction and microthrombin can be found in the early stage of essential hypertension. Both enalapril and terazosin could improve the endothelial function and prothrombotic state by promoting the synthesis of nitric oxide.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"382-4"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24106035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[A short-term trial of Didanosine, stavudine, and nevirapine combination therapy for human immunodeficiency virus infection]. 二腺苷、司他夫定和奈韦拉平联合治疗人类免疫缺陷病毒感染的短期试验

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Yu-huang Zheng, Xu Yang, Wei-hong Chang

引用次数: 0

[Effects of lithium on the activity of ERK-1/2 signal pathway and expression of Bcl-2 family proteins in the central nervous system in vivo]. [锂对体内中枢神经系统ERK-1/2信号通路活性及Bcl-2家族蛋白表达的影响]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Jian-ling Li, Zhao-zheng Jing, Hong Yi

{"title":"[Effects of lithium on the activity of ERK-1/2 signal pathway and expression of Bcl-2 family proteins in the central nervous system in vivo].","authors":"Jian-ling Li, Zhao-zheng Jing, Hong Yi","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the effects of chronic in vivo lithium administration on ERK-1/2 signal pathway and the expression of Bcl-2 family proteins, and to elucidate the molecular mechanisms underlying the therapeutic effects of lithium on the manic depression.Methods: Forty male Wistar rats were evenly divided into 2 groups. One group was treated with 2.4 g/kg of Li2CO3 for 4 weeks. The other group were given with normal chow. By the end of the 4th week, rat brains were removed immediately on decapitation and dissected on ice. The total proteins of rat brain hippocampus and frontal cortex were prepared. Levels of the prylated, active forms of MEK, ERK-1/2, RSK1 and CREB, and the expression levels of Bcl-2 family were assayed by Western Blotting.Results: Lithium increased the activities of MEK, ERK-1/2, RSK1, and CREB, up-regulated the expression of Bcl-2, and down-regulated the expression of Bax in rat brain hippocampus and frontal cortex.Conclusion: Chronic in vivo lithium administration activities ERK-1/2 signal pathway and regulates the expression of Bcl-2 family proteins in the central nervous system, which may associate with the therapeutic effects of lithium in the treatment of manic depression.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"330-4"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24104921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0