Human Fertility最新文献_第6页

Ovarian sensitivity index can be used as a more sensitive indicator than follicular output rate to predict IVF/ICSI outcomes in patients of normal expected ovarian response stimulated with GnRH antagonist protocol. 卵巢敏感性指数可作为比卵泡排出率更灵敏的指标，用于预测使用 GnRH 拮抗剂方案刺激的正常预期卵巢反应患者的 IVF/ICSI 结果。

IF 1.9 4区医学

Human Fertility Pub Date : 2023-12-01 Epub Date: 2023-01-17 DOI: 10.1080/14647273.2023.2164869

Fen Hu, Xiaocheng Wang, Haiying Ren, Yasu Lv, Huanhuan Li, Shan Liu, Wenhui Zhou

{"title":"Ovarian sensitivity index can be used as a more sensitive indicator than follicular output rate to predict IVF/ICSI outcomes in patients of normal expected ovarian response stimulated with GnRH antagonist protocol.","authors":"Fen Hu, Xiaocheng Wang, Haiying Ren, Yasu Lv, Huanhuan Li, Shan Liu, Wenhui Zhou","doi":"10.1080/14647273.2023.2164869","DOIUrl":"10.1080/14647273.2023.2164869","url":null,"abstract":"This retrospective study was performed to investigate the predictive power of the Ovarian Sensitivity Index (OSI) for IVF/ICSI outcomes in infertile patients who were of normal expected ovarian response. A total of 912 infertile patients who underwent GnRH antagonist protocol between January 2017 to August 2019 at the Medical Center for Human Reproduction, Beijing Chao-Yang Hospital were included. All patients completed the full oocyte retrieval cycle and either had a live birth or had no embryos left. OSI was significantly lower in patients with a live birth (196.0 ± 120.4 in the live birth group vs 276.4 ± 235.7 in the non-live birth group, p < 0.001) while follicular output rate (FORT, defined as the ratio of pre-ovulatory follicle count on hCG day x 100/small antral follicle count at baseline) showed no significant difference. Patients were divided into low, average and high OSI groups and analysed in tertiles. From the low to the high OSI group, the cumulative live birth rate (CLBR) decreased dramatically (72.7 vs 67.2 vs 54.8%, p < 0.001). Multivariate regression analysis showed that OSI was an independent factor affecting CLBR (OR: 0.996, 95%CI: 0.995-0.998, p < 0.001) in our study population. In conclusion, OSI can be used as an independent indicator to distinguish fecundity in infertile patients with normal expected ovarian response and is probably more sensitive than FORT.","PeriodicalId":13006,"journal":{"name":"Human Fertility","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10539382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of artificial neural networks in reproductive medicine. 人工神经网络在生殖医学中的应用。

IF 1.9 4区医学

Human Fertility Pub Date : 2023-12-01 Epub Date: 2023-01-11 DOI: 10.1080/14647273.2022.2156301

Guanghui Yuan, Bohan Lv, Cuifang Hao

引用次数: 0

Fertility preservation discussions, referral and follow-up in male-to-female and female-to-male adolescent transgender patients. 男变女和女变男青少年变性患者的生育力保护讨论、转诊和随访。

IF 1.9 4区医学

Human Fertility Pub Date : 2023-12-01 Epub Date: 2021-12-17 DOI: 10.1080/14647273.2021.2015804

Allison S Komorowski, Andrew R Fisher, Emily S Jungheim, Christopher S Lewis, Kenan R Omurtag

{"title":"Fertility preservation discussions, referral and follow-up in male-to-female and female-to-male adolescent transgender patients.","authors":"Allison S Komorowski, Andrew R Fisher, Emily S Jungheim, Christopher S Lewis, Kenan R Omurtag","doi":"10.1080/14647273.2021.2015804","DOIUrl":"10.1080/14647273.2021.2015804","url":null,"abstract":"The number of patients seeking transgender healthcare is growing, and there is a potential impact of gender-affirming therapies on fertility. The use of fertility preservation (FP), particularly among transgender adolescents, has been limited. We aimed to examine differences in FP counselling, referral and utilisation between male-to-female (MtF) and female-to-male (FtM) transgender adolescents. A retrospective review of the medical records of patients ages 12-17 seen at an academic medical centre between 2012 and 2017 with a diagnosis of gender dysphoria was conducted. A total of 22 MtF and 45 FtM adolescents were included. The counselling on the potential fertility impact of gender-affirming therapy was documented in 55%, and of those counselled, 73% were counselled before receiving medication. There was no significant difference between the timing of counselling for MtF versus FtM adolescents. Of patients with documented reproductive wishes, 77% reported either desire for adopted children or no desire for biological children. Among patients offered FP referral, 2 (22.2%) MtF and 3 (12.5%) FtM patients accepted; both MtF patients cryopreserved sperm. While most adolescents were counselled on the fertility impact of gender-affirming therapy, there is room for improvement as 45% of patients had no documented counselling. The rate of transgender adolescents pursuing FP consultation and gamete cryopreservation was low, consistent with prior studies in this population.","PeriodicalId":13006,"journal":{"name":"Human Fertility","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39732433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Impact of body mass index on peripheral and uterine immune status in the window of implantation in patients with recurrent reproductive failure. 体重指数对反复生殖失败患者植入窗口期外周和子宫免疫状态的影响。

IF 1.9 4区医学

Human Fertility Pub Date : 2023-12-01 Epub Date: 2023-03-22 DOI: 10.1080/14647273.2023.2189024

ShuYi Yu, RuoChun Lian, Cong Chen, Xian Chen, Jian Xu, Yong Zeng, YuYe Li

{"title":"Impact of body mass index on peripheral and uterine immune status in the window of implantation in patients with recurrent reproductive failure.","authors":"ShuYi Yu, RuoChun Lian, Cong Chen, Xian Chen, Jian Xu, Yong Zeng, YuYe Li","doi":"10.1080/14647273.2023.2189024","DOIUrl":"10.1080/14647273.2023.2189024","url":null,"abstract":"This study aimed to investigate whether maternal obesity affects the immune status of peripheral blood and endometrium in patients with recurrent reproductive failure classified according to their body mass index (BMI). A total of 228 repeated implantation failure (RIF) and 266 recurrent miscarriage (RM) patients were enrolled in the study and further subdivided into three groups according to their BMI: (i) normal weight (18.5≤ BMI <23); (ii) overweight (23≤ BMI <25); and (iii) obese (BMI ≥25). Peripheral blood and endometrium samples were collected in the mid-luteal phase before IVF treatment or natural pregnancy. Peripheral immunocytes were analyzed by flow cytometry, while uterine immune cells were subjected to immunohistochemistry. In RM patients, significantly increased peripheral helper T cells and decreased cytotoxic T cells, NK cells were observed in the obese group compared with the normal-weight group. Meanwhile, in the endometrium, the percentage of NK cell, macrophage cell, M2 macrophage cell, and Treg cell significantly reduced with increased BMI in RIF patients, and the percentage of NK cell and M2 macrophage cell significantly decreased with increased BMI in RM patients. In conclusion, obesity may cause endometrial immune disorder in recurrent reproductive failure women, but was only associated with the peripheral immune change in RM patients.","PeriodicalId":13006,"journal":{"name":"Human Fertility","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9156735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MACS-DGC sperm preparation method resulted in high-quality sperm, top-quality embryo, and higher blastocyst rate in male factor infertile couples with high DNA fragmented sperm. MACS-DGC 精子制备法可为高 DNA 碎片精子的男性因素不育夫妇提供优质精子、优质胚胎和更高的囊胚率。

IF 1.9 4区医学

Human Fertility Pub Date : 2023-12-01 Epub Date: 2023-07-20 DOI: 10.1080/14647273.2023.2236297

Maryam Salehi Novin, Anahita Mehdizadeh, Tayebe Artimani, Mehrdad Bakhtiari, Mehdi Mehdizadeh, Reza Aflatoonian, Zahra Zandieh

{"title":"MACS-DGC sperm preparation method resulted in high-quality sperm, top-quality embryo, and higher blastocyst rate in male factor infertile couples with high DNA fragmented sperm.","authors":"Maryam Salehi Novin, Anahita Mehdizadeh, Tayebe Artimani, Mehrdad Bakhtiari, Mehdi Mehdizadeh, Reza Aflatoonian, Zahra Zandieh","doi":"10.1080/14647273.2023.2236297","DOIUrl":"10.1080/14647273.2023.2236297","url":null,"abstract":"Conventional sperm selection based on motility and morphology fails to provide detailed information on sperm functional and molecular status. Magnetic-activated cell sorting (MACS) protocol aims to optimize this process by selecting apoptotic sperm cells. Phospholipase C zeta-1 (PLCz1) is a physiological stimulus for oocyte activation and early embryonic development. The purpose of this study was to examine seminal parameters, DNA fragmentation index (DFI), and PLCz1 expression levels in MACS-DGC sorted specimens (DFI > 30%) and assess early development in resulting embryos. Semen specimens from 60 patients diagnosed with male factor infertility were collected and processed by either density gradient centrifugation (DGC) or MACS-DGC protocols. Pre and post-preparation analysis was performed. PLCz1 expression was assessed using the RT-PCR method. Retrieved eggs from their partners were divided into two groups in which they were injected with different sorted sperm. The fertilization rate and embryonic development were evaluated. While sperm's progressive motility and morphology significantly improved, there was a substantial decline in DFI following MACS-DGC. Fertilization rates were almost the same between the groups, and the latter resulted in remarkably more top-quality embryos and more blastocysts. PLCz1 expression was considerably higher in the MACS-DGC group. By eliminating apoptotic cells, the MACS-DGC technique could sort highly PLCz1-expressed sperm, optimize sperm selection in individuals with elevated DFI, development of resulting embryos.","PeriodicalId":13006,"journal":{"name":"Human Fertility","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9837428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictors and differences in fertility level among Nigerian women of reproductive age: a function of subgroup social norms fertility behaviour. 尼日利亚育龄妇女生育水平的预测因素和差异：亚群体社会规范生育行为的作用。

IF 1.9 4区医学

Human Fertility Pub Date : 2023-12-01 Epub Date: 2022-11-12 DOI: 10.1080/14647273.2022.2137859

Olukemi Grace Adebola, Olusegun Sunday Ewemooje, Femi Barnabas Adebola

{"title":"Predictors and differences in fertility level among Nigerian women of reproductive age: a function of subgroup social norms fertility behaviour.","authors":"Olukemi Grace Adebola, Olusegun Sunday Ewemooje, Femi Barnabas Adebola","doi":"10.1080/14647273.2022.2137859","DOIUrl":"10.1080/14647273.2022.2137859","url":null,"abstract":"Fertility rates in Nigeria are declining at such a modest rate, that if more proactive measures are not employed to reduce fertility, the nation may double its population before long. We empirically examined fertility behaviour as derivatives of specific subgroup social norms, and the variations in the factors responsible for different fertility behaviours, using the 2018 Nigeria Demographic and Health Survey. Descriptive bivariate, and multinomial logistic regression analyses were used to predict the contribution of demographic and sociocultural factors contributing to the fertility level, and findings were reported as odds ratios. Results revealed that age, religion and level of education are the most significant predictors of fertility level, with remarkable differences in birth rate across subgroups, whereas North-West Nigeria had the highest fertility level. Furthermore, marriage had a significantly negative effect on high fertility among North-Western women, whereas higher education significantly increased high fertility among North-Eastern women. In conclusion, subgroup social norms fertility behaviour is responsible for the persistent fertility differential outcome in Nigeria. Thus, the paper strongly advocates the need to intensify community-led, norm-based solution and not a universal approach in addressing fertility control in Nigeria.","PeriodicalId":13006,"journal":{"name":"Human Fertility","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10747662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of vascular endothelial growth factor A (VEGFA), placental growth factor (PlGF) and insulin-like growth factor 1 (IGF-1) in serum from women undergoing frozen embryo transfer. 冷冻胚胎移植妇女血清中血管内皮生长因子A (VEGFA)、胎盘生长因子(PlGF)和胰岛素样生长因子1 (IGF-1)的表达

IF 1.9 4区医学

Human Fertility Pub Date : 2023-12-01 Epub Date: 2022-03-04 DOI: 10.1080/14647273.2022.2040749

Xi Guo, Chi Chiu Wang, Jacqueline Pui Wah Chung, Tin Chiu Li, Xiaoyan Chen

{"title":"Expression of vascular endothelial growth factor A (VEGFA), placental growth factor (PlGF) and insulin-like growth factor 1 (IGF-1) in serum from women undergoing frozen embryo transfer.","authors":"Xi Guo, Chi Chiu Wang, Jacqueline Pui Wah Chung, Tin Chiu Li, Xiaoyan Chen","doi":"10.1080/14647273.2022.2040749","DOIUrl":"10.1080/14647273.2022.2040749","url":null,"abstract":"VEGFA, PlGF and IGF-1 are three main angiogenic factors which play significant roles in embryo implantation. However, the relationship between serum expressions of VEGFA, PlGF and IGF-1 and pregnancy outcomes has not been fully illustrated. In this study, serum specimens were collected precisely on day 7 after the LH surge in a natural non-conception cycle from 38 infertile patients who underwent frozen embryo transfer (FET) treatment. ELISA was used to determine the concentrations of VEGFA, PlGF and IGF-1. Serum levels of VEGFA, PlGF and IGF-1 were compared between patients who conceived (n=25) and who did not (n=13). Correlation and linear regression analyses were used to investigate the correlations of serum angiogenic factors and β-hCG MoM levels in the pregnant group. The results demonstrated that no significant difference was found in serum VEGFA, PlGF or IGF-1 concentration between pregnant and non-pregnant women. Spearman correlation analysis revealed a positive correlation between IGF-1 concentration and β-hCG level in pregnant participants (rs = 0.490, p = 0.013). In conclusion, serum IGF-1 level correlated positively with β-hCG level in pregnant women, which may provide information on the prognostic value of IGF-1 in this group of women.","PeriodicalId":13006,"journal":{"name":"Human Fertility","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43792434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal and child-health outcomes in different endometrial preparation methods for frozen-thawed embryo transfer: a retrospective study. 冻融胚胎移植中不同子宫内膜制备方法对母婴健康的影响:回顾性研究

IF 1.9 4区医学

Human Fertility Pub Date : 2023-12-01 Epub Date: 2022-03-28 DOI: 10.1080/14647273.2022.2053593

Xin Li, Qijun Xie, Ting Luan, Yan Su, Juan Zhang, JuanJuan Zhang, Chun Zhao, Xiufeng Ling

{"title":"Maternal and child-health outcomes in different endometrial preparation methods for frozen-thawed embryo transfer: a retrospective study.","authors":"Xin Li, Qijun Xie, Ting Luan, Yan Su, Juan Zhang, JuanJuan Zhang, Chun Zhao, Xiufeng Ling","doi":"10.1080/14647273.2022.2053593","DOIUrl":"10.1080/14647273.2022.2053593","url":null,"abstract":"The purpose of this study was to analyse the obstetric outcome after frozen embryo transfer depending on the protocol used. The endometrial preparation during frozen embryo transfer (FET) was performed in the natural cycle (NC) with timing based on monitoring of the naturally occurring luteinizing hormone (LH) peak or in human chorionic gonadotropin (hCG)-triggered modified natural cycles (MNC), artificial cycle (AC) with hormone replacement therapy cycle and cycle with ovulation induction (OI). A retrospective cohort study was conducted between 2018 and 2020. Pregnancy, perinatal complications and neonatal outcomes were compared among patients treated during AC or MNC or OI protocols. After adjusting for the effect of gravidity, parity, pre-pregnancy body mass index (BMI) and number of miscarriages, the AC group had higher but not significantly different rates of gestational hypertension, preeclampsia and intrahepatic cholestasis of pregnancy (ICP) than women in OI and MNC groups. Significant differences were observed in the rates of placental adherence (15.30% in AC vs. 9.24% in MNC, p = 0.004) and postpartum haemorrhage (PPH) (24.33% in AC vs. 13.07% in OI, p = 0.003, 24.33% in AC vs. 16.24% in MNC, p = 0.002) among the three groups. In singletons, significant differences were observed in the rates of placental adherence (14.09% in AC vs. 8.57% in MNC, p = 0.002), AC and MNC groups had higher risk of PPH compared with OI group (18.36% in AC vs. 12.38% in MNC, p = 0.042 and 7.69% in OI vs. 18.36% in AC, p = 0.013). Neonatal outcomes were similar among the three groups except risk of small for date infants (0.35% in AC vs. 1.44% in MNC, p = 0.008). These findings are important in view of increasing use of frozen cycles and new policy of freeze-all cycles in in vitro fertilization (IVF). The results suggest a link between the absence of corpus luteum and adverse obstetric outcomes.","PeriodicalId":13006,"journal":{"name":"Human Fertility","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42424423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Clomiphene citrate priming increases sensitivity during ovarian stimulation in poor ovarian responders undergoing in vitro fertilization treatment: a retrospective cohort study. 一项回顾性队列研究：枸橼酸氯米芬引物可提高接受体外受精治疗的卵巢反应差者在卵巢刺激过程中的敏感性。

IF 1.9 4区医学

Human Fertility Pub Date : 2023-12-01 Epub Date: 2022-08-11 DOI: 10.1080/14647273.2022.2109069

Shan Liu, Xinyu Liu, Huanhuan Li, Minghui Liu, Yasu Lv, Yuan Li

{"title":"Clomiphene citrate priming increases sensitivity during ovarian stimulation in poor ovarian responders undergoing in vitro fertilization treatment: a retrospective cohort study.","authors":"Shan Liu, Xinyu Liu, Huanhuan Li, Minghui Liu, Yasu Lv, Yuan Li","doi":"10.1080/14647273.2022.2109069","DOIUrl":"10.1080/14647273.2022.2109069","url":null,"abstract":"This single-centre retrospective cohort study aimed to investigate whether a clomiphene citrate (CC) priming protocol could increase ovarian sensitivity in poor ovarian responders. It included 294 patients (374 ovarian stimulation cycles). Of these, 193 cycles were treated by a CC priming antagonist protocol (study group) and 181 by the classical flexible gonadotropin-releasing hormone antagonist protocol (control group). Stimulation data and laboratory and clinical outcomes were compared between the groups. The results showed that in the study group, total gonadotropin dosage and dosage per follicle were considerably lower, the follicle-to-oocyte index was significantly higher, and the gonadotropin duration was shorter. After adjusting for potential confounders, multivariate regression analysis showed that cumulative ongoing pregnancy remained comparable between the groups (adjusted odds ratio: 0.761, 95% confidence interval: 0.300-1.933, p = 0.566). Age, body mass index, gonadotropin dosage per follicle, and the follicle-to-oocyte index were negatively associated with the reproductive outcomes. The result of the sensitivity analysis showed that patients in the study group were administered less gonadotropin at a lower gonadotropin dosage per follicle and for a shorter duration. In conclusion, the CC priming antagonist protocol offered a convenient and patient-friendly way to increase ovarian sensitivity during ovarian stimulation in poor ovarian responders.","PeriodicalId":13006,"journal":{"name":"Human Fertility","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40685132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of potential key genes related to idiopathic male infertility using RNA-sequencing data: an in-silico approach. 利用 RNA 测序数据鉴定与特发性男性不育症相关的潜在关键基因：一种内部方法。

IF 1.9 4区医学

Human Fertility Pub Date : 2023-12-01 Epub Date: 2022-11-12 DOI: 10.1080/14647273.2022.2144771

Laxman Kumar Lukkani, Leimarembi Devi Naorem, Mathavan Muthaiyan, Amouda Venkatesan

{"title":"Identification of potential key genes related to idiopathic male infertility using RNA-sequencing data: an in-silico approach.","authors":"Laxman Kumar Lukkani, Leimarembi Devi Naorem, Mathavan Muthaiyan, Amouda Venkatesan","doi":"10.1080/14647273.2022.2144771","DOIUrl":"10.1080/14647273.2022.2144771","url":null,"abstract":"Among reproductive health problems, idiopathic infertility affects married couples. The current diagnosis of male infertility focuses on the concentration, motility, and morphology of sperm in the ejaculate. Since the molecular mechanism of idiopathic infertility is unknown, identification of Differentially Expressed Genes (DEGs) among the control and idiopathic infertile male can shed light on diagnosis and treatment. Here, we analyzed the dataset GSE65683 to identify DEGs in idiopathic human sperm in three groups of patients: (i) Timed Intercourse (TIC); (ii) Intrauterine Insemination (IUI); and (iii) Assisted Reproductive Technology (ART). The enrichment analysis was carried out using DAVID (Database for Annotation, Visualization and Integrated Discovery) and GeneCodis for the DEGs. Protein-Protein Interaction (PPI) network of these DEGs were constructed using the STRING database. The network parameters such as degree and betweenness were calculated to select the important hubs. In total, 118 DEGs in TIC, 446 in IUI, and 188 in ART were identified. PPI network was constructed and identified critical top hub genes such as ACTB, BTBD6, EIF2S3, EIF3A, EIF4E, POLR2L, RPL4, RPL7, RPS11, RPL13, RPS15, RPL23, RPL27, RPL9, RPLP0 and UBA52 that may play an essential role in idiopathic male infertility. Thus, the identified hub genes may provide an insight into the molecular mechanism and contribute to discovering novel therapeutic targets and developing new strategies for idiopathic male infertility.","PeriodicalId":13006,"journal":{"name":"Human Fertility","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40473110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1