Gut Microbes最新文献_第9页

EspH utilizes phosphoinositide and Rab binding domains to interact with plasma membrane infection sites and Rab GTPases. EspH 利用磷脂和 Rab 结合域与质膜感染位点和 Rab GTPases 相互作用。

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-01-01 Epub Date: 2024-09-23 DOI: 10.1080/19490976.2024.2400575

Ipsita Nandi, Rachana Pattani Ramachandran, Deborah E Shalev, Dina Schneidman-Duhovny, Raisa Shtuhin-Rahav, Naomi Melamed-Book, Efrat Zlotkin-Rivkin, Alexander Rouvinski, Ilan Rosenshine, Benjamin Aroeti

{"title":"EspH utilizes phosphoinositide and Rab binding domains to interact with plasma membrane infection sites and Rab GTPases.","authors":"Ipsita Nandi, Rachana Pattani Ramachandran, Deborah E Shalev, Dina Schneidman-Duhovny, Raisa Shtuhin-Rahav, Naomi Melamed-Book, Efrat Zlotkin-Rivkin, Alexander Rouvinski, Ilan Rosenshine, Benjamin Aroeti","doi":"10.1080/19490976.2024.2400575","DOIUrl":"10.1080/19490976.2024.2400575","url":null,"abstract":"Enteropathogenic E. coli (EPEC) is a Gram-negative bacterial pathogen that causes persistent diarrhea. Upon attachment to the apical plasma membrane of the intestinal epithelium, the pathogen translocates virulence proteins called effectors into the infected cells. These effectors hijack numerous host processes for the pathogen's benefit. Therefore, studying the mechanisms underlying their action is crucial for a better understanding of the disease. We show that translocated EspH interacts with multiple host Rab GTPases. AlphaFold predictions and site-directed mutagenesis identified glutamic acid and lysine at positions 37 and 41 as Rab interacting residues in EspH. Mutating these sites abolished the ability of EspH to inhibit Akt and mTORC1 signaling, lysosomal exocytosis, and bacterial invasion. Knocking out the endogenous Rab8a gene expression highlighted the involvement of Rab8a in Akt/mTORC1 signaling and lysosomal exocytosis. A phosphoinositide binding domain with a critical tyrosine was identified in EspH. Mutating the tyrosine abolished the localization of EspH at infection sites and its capacity to interact with the Rabs. Our data suggest novel EspH-dependent mechanisms that elicit immune signaling and membrane trafficking during EPEC infection.","PeriodicalId":12909,"journal":{"name":"Gut Microbes","volume":"16 1","pages":"2400575"},"PeriodicalIF":12.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11421376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of gut microbiota on appetite regulation and the underlying mechanisms. 肠道微生物群对食欲调节的影响及其内在机制。

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-01-01 Epub Date: 2024-11-06 DOI: 10.1080/19490976.2024.2414796

Miao Yu, Bing Yu, Daiwen Chen

{"title":"The effects of gut microbiota on appetite regulation and the underlying mechanisms.","authors":"Miao Yu, Bing Yu, Daiwen Chen","doi":"10.1080/19490976.2024.2414796","DOIUrl":"10.1080/19490976.2024.2414796","url":null,"abstract":"Appetite, a crucial aspect regulated by both the central nervous system and peripheral hormones, is influenced by the composition and dynamics of the intestinal microbiota, as evidenced by recent research. This review highlights the role of intestinal microbiota in appetite regulation, elucidating the involvement of various pathways. Notably, the metabolites generated by intestinal microorganisms, including short-chain fatty acids, bile acids, and amino acid derivatives, play a pivotal role in this intricate process. Furthermore, intestinal microorganisms contribute to appetite regulation by modulating nutritional perception, neural signal transmission, and hormone secretion within the digestive system. Consequently, manipulating and modulating the intestinal microbiota represent innovative strategies for ameliorating appetite-related disorders. This paper provides a comprehensive review of the effects of gut microbes and their metabolites on the central nervous system and host appetite. By exploring their potential regulatory pathways and mechanisms, this study aims to enhance our understanding of how gut microbes influence appetite regulation in the host.","PeriodicalId":12909,"journal":{"name":"Gut Microbes","volume":"16 1","pages":"2414796"},"PeriodicalIF":12.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11542600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary fiber intake impacts gut bacterial and viral populations in a hypertensive mouse model. 膳食纤维摄入量影响高血压小鼠模型中的肠道细菌和病毒群。

IF 2.9 1区医学

Gut Microbes Pub Date : 2024-01-01 Epub Date: 2024-09-28 DOI: 10.1080/19490976.2024.2407047

Laura Avellaneda-Franco, Liang Xie, Michael Nakai, Jeremy J Barr, Francine Z Marques

{"title":"Dietary fiber intake impacts gut bacterial and viral populations in a hypertensive mouse model.","authors":"Laura Avellaneda-Franco, Liang Xie, Michael Nakai, Jeremy J Barr, Francine Z Marques","doi":"10.1080/19490976.2024.2407047","DOIUrl":"10.1080/19490976.2024.2407047","url":null,"abstract":"The gut microbiome is an emerging factor in preventing hypertension, yet the influence of gut bacteriophages, viruses infecting bacteria, on this condition remains unclear. Bacteriophage-bacteria interactions, which impact the gut microbiome, are influenced differentially by temperate and virulent bacteriophages. However, the standard technique for studying viral populations, viral-like particles (VLPs)-metagenomes, often overlook prophages, the intracellular stage of temperate bacteriophages, creating a knowledge gap. To address this, we investigated alterations in extracellular and intracellular bacteriophages, alongside bacterial populations, in the angiotensin II-hypertension model. We sequenced VLPs and bulk DNA from cecal-colonic samples collected from male C57BL/6J mice implanted with minipumps containing saline or angiotensin II. We assembled 106 bacterial and 816 viral genomes and found that gut viral and bacterial populations remained stable between hypertensive and normotensive mice. A higher number of temperate viruses were observed across all treatments. Although temperate viruses outnumbered virulent viruses, sequencing of both VLPs and bulk revealed that virions from virulent viruses were more abundant in the murine gut. We then evaluated the impact of low- and high-fiber intake on gut microbiome composition in the angiotensin II model. Fiber intake significantly influenced the gut microbiome composition and hypertension development. Mice receiving high-fiber had lower blood pressure, a higher bacterial-encoded carbohydrate-associated enzyme, and a higher total relative abundance of temperate viruses than those receiving low-fiber. Our findings suggest that phages are not associated with hypertension development in the angiotensin II model. However, they support a complex diet-bacteria/phage interaction that may be involved in blood pressure regulation.","PeriodicalId":12909,"journal":{"name":"Gut Microbes","volume":"16 1","pages":"2407047"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11567275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

B. thetaiotaomicron-derived acetic acid modulate immune microenvironment and tumor growth in hepatocellular carcinoma. B. Thetaiotaomicron衍生的乙酸调节肝细胞癌的免疫微环境和肿瘤生长。

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-01-01 Epub Date: 2024-01-25 DOI: 10.1080/19490976.2023.2297846

Hongbin Ma, Liang Yang, Yingchao Liang, Fenghua Liu, Jinxiang Hu, Rui Zhang, Yong Li, Lei Yuan, Feiling Feng

{"title":"B. thetaiotaomicron-derived acetic acid modulate immune microenvironment and tumor growth in hepatocellular carcinoma.","authors":"Hongbin Ma, Liang Yang, Yingchao Liang, Fenghua Liu, Jinxiang Hu, Rui Zhang, Yong Li, Lei Yuan, Feiling Feng","doi":"10.1080/19490976.2023.2297846","DOIUrl":"10.1080/19490976.2023.2297846","url":null,"abstract":"Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths worldwide, and emerging evidence suggests that the gut microbiota may play a role in its development and progression. In this study, the association between B. thetaiotaomicron, a gut microbiota species, and HCC recurrence, as well as patient clinical outcomes, was investigated. It was observed that B. thetaiotaomicron-derived acetic acid has the potential to modulate the polarization of pro-pro-inflammatory macrophagess, which promotes the function of cytotoxic CD8+ T cells. The increased biosynthesis of fatty acids was implicated in the modulation of pro-inflammatory macrophages polarization by B. thetaiotaomicron-derived acetic acid. Furthermore, B. thetaiotaomicron-derived acetic acid was found to facilitate the transcription of ACC1, a key enzyme involved in fatty acid biosynthesis, through histone acetylation modification in the ACC1 promoter region. Curcumin, an acetylation modification inhibitor, significantly blocked the inhibitory effects of B. thetaiotaomicron and acetic acid on HCC tumor growth. These findings highlight the potential role of gut microbiota-derived acetic acid in HCC recurrence and patient clinical outcomes, and suggest a complex interplay between gut microbiota, immune modulation, fatty acid metabolism, and epigenetic regulation in the context of HCC development. Further research in this area may provide insights into novel strategies for HCC prevention and treatment by targeting the gut microbiota and its metabolites.","PeriodicalId":12909,"journal":{"name":"Gut Microbes","volume":"16 1","pages":"2297846"},"PeriodicalIF":12.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10813637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139546120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the hidden players: exploring the role of gut mycobiome in cancer development and treatment dynamics. 揭开隐藏角色的面纱：探索肠道霉菌生物群在癌症发展和治疗动态中的作用。

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-01-01 Epub Date: 2024-03-14 DOI: 10.1080/19490976.2024.2328868

Lingxi Li, Xiaowen Huang, Haoyan Chen

引用次数: 0

Microbiota-derived I3A protects the intestine against radiation injury by activating AhR/IL-10/Wnt signaling and enhancing the abundance of probiotics. 微生物群衍生的 I3A 可通过激活 AhR/IL-10/Wnt 信号和提高益生菌的丰度来保护肠道免受辐射损伤。

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-01-01 Epub Date: 2024-05-05 DOI: 10.1080/19490976.2024.2347722

Li-Wei Xie, Shang Cai, Hai-Yan Lu, Feng-Ling Tang, Rui-Qiu Zhu, Ye Tian, Ming Li

{"title":"Microbiota-derived I3A protects the intestine against radiation injury by activating AhR/IL-10/Wnt signaling and enhancing the abundance of probiotics.","authors":"Li-Wei Xie, Shang Cai, Hai-Yan Lu, Feng-Ling Tang, Rui-Qiu Zhu, Ye Tian, Ming Li","doi":"10.1080/19490976.2024.2347722","DOIUrl":"10.1080/19490976.2024.2347722","url":null,"abstract":"The intestine is prone to radiation damage in patients undergoing radiotherapy for pelvic tumors. However, there are currently no effective drugs available for the prevention or treatment of radiation-induced enteropathy (RIE). In this study, we aimed at investigating the impact of indole-3-carboxaldehyde (I3A) derived from the intestinal microbiota on RIE. Intestinal organoids were isolated and cultivated for screening radioprotective tryptophan metabolites. A RIE model was established using 13 Gy whole-abdominal irradiation in male C57BL/6J mice. After oral administration of I3A, its radioprotective ability was assessed through the observation of survival rates, clinical scores, and pathological analysis. Intestinal stem cell survival and changes in the intestinal barrier were observed through immunofluorescence and immunohistochemistry. Subsequently, the radioprotective mechanisms of I3A was investigated through 16S rRNA and transcriptome sequencing, respectively. Finally, human colon cancer cells and organoids were cultured to assess the influence of I3A on tumor radiotherapy. I3A exhibited the most potent radioprotective effect on intestinal organoids. Oral administration of I3A treatment significantly increased the survival rate in irradiated mice, improved clinical and histological scores, mitigated mucosal damage, enhanced the proliferation and differentiation of Lgr5+ intestinal stem cells, and maintained intestinal barrier integrity. Furthermore, I3A enhanced the abundance of probiotics, and activated the AhR/IL-10/Wnt signaling pathway to promote intestinal epithelial proliferation. As a crucial tryptophan metabolite, I3A promotes intestinal epithelial cell proliferation through the AhR/IL-10/Wnt signaling pathway and upregulates the abundance of probiotics to treat RIE. Microbiota-derived I3A demonstrates potential clinical application value for the treatment of RIE.","PeriodicalId":12909,"journal":{"name":"Gut Microbes","volume":"16 1","pages":"2347722"},"PeriodicalIF":12.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11086037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Akkermansia muciniphila: a deworming partner independent of type 2 immunity. Akkermansia muciniphila：独立于 2 型免疫的驱虫伴侣。

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-01-01 Epub Date: 2024-05-08 DOI: 10.1080/19490976.2024.2338947

Jiaqi Wang, Xiufeng Zhao, Xianhe Li, Xuemin Jin

引用次数: 0

Prevotella copri promotes vascular calcification via lipopolysaccharide through activation of NF-κB signaling pathway. 前驱菌通过激活 NF-κB 信号通路，通过脂多糖促进血管钙化。

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-01-01 Epub Date: 2024-05-10 DOI: 10.1080/19490976.2024.2351532

Qing-Yun Hao, Jing Yan, Jin-Tao Wei, Yu-Hong Zeng, Li-Yun Feng, Dong-Dong Que, Shi-Chao Li, Jing-Bin Guo, Ying Fan, Yun-Fa Ding, Xiu-Li Zhang, Ping-Zhen Yang, Jing-Wei Gao, Ze-Hua Li

{"title":"Prevotella copri promotes vascular calcification via lipopolysaccharide through activation of NF-κB signaling pathway.","authors":"Qing-Yun Hao, Jing Yan, Jin-Tao Wei, Yu-Hong Zeng, Li-Yun Feng, Dong-Dong Que, Shi-Chao Li, Jing-Bin Guo, Ying Fan, Yun-Fa Ding, Xiu-Li Zhang, Ping-Zhen Yang, Jing-Wei Gao, Ze-Hua Li","doi":"10.1080/19490976.2024.2351532","DOIUrl":"10.1080/19490976.2024.2351532","url":null,"abstract":"Emerging evidence indicates that alteration of gut microbiota plays an important role in chronic kidney disease (CKD)-related vascular calcification (VC). We aimed to investigate the specific gut microbiota and the underlying mechanism involved in CKD-VC. We identified an increased abundance of Prevotella copri (P. copri) in the feces of CKD rats (induced by using 5/6 nephrectomy followed by a high calcium and phosphate diet) with aortic calcification via amplicon sequencing of 16S rRNA genes. In patients with CKD, we further confirmed a positive correlation between abundance of P. copri and aortic calcification scores. Moreover, oral administration of live P. copri aggravated CKD-related VC and osteogenic differentiation of vascular smooth muscle cells in vivo, accompanied by intestinal destruction, enhanced expression of Toll-like receptor-4 (TLR4), and elevated lipopolysaccharide (LPS) levels. In vitro and ex vivo experiments consistently demonstrated that P. copri-derived LPS (Pc-LPS) accelerated high phosphate-induced VC and VSMC osteogenic differentiation. Mechanistically, Pc-LPS bound to TLR4, then activated the nuclear factor κB (NF-κB) and nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 (NLRP3) inflammasome signals during VC. Inhibition of NF-κB reduced NLRP3 inflammasome and attenuated Pc-LPS-induced VSMC calcification. Our study clarifies a novel role of P. copri in CKD-related VC, by the mechanisms involving increased inflammation-regulating metabolites including Pc-LPS, and activation of the NF-κB/NLRP3 signaling pathway. These findings highlight P. copri and its-derived LPS as potential therapeutic targets for VC in CKD.","PeriodicalId":12909,"journal":{"name":"Gut Microbes","volume":"16 1","pages":"2351532"},"PeriodicalIF":12.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11093026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiota transplantation in restoring cesarean-related infant dysbiosis: a new frontier. 微生物群移植用于恢复与剖腹产相关的婴儿菌群失调：一个新领域。

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-01-01 Epub Date: 2024-05-15 DOI: 10.1080/19490976.2024.2351503

Sina Liu, Xiaoxia Luo, Lepeng Zhou, Ri-Hua Xie, Yan He

引用次数: 0

The transplantation of the gut microbiome of fat-1 mice protects against colonic mucus layer disruption and endoplasmic reticulum stress induced by high fat diet. 移植脂肪-1 小鼠的肠道微生物群可防止高脂肪饮食引起的结肠粘液层破坏和内质网应激。

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-01-01 Epub Date: 2024-05-26 DOI: 10.1080/19490976.2024.2356270

Amina Bourragat, Quentin Escoula, Sandrine Bellenger, Olivier Zemb, Martin Beaumont, Killian Chaumonnot, Jean-Pierre Farine, Emmanuel Jacotot, Aline Bonnotte, Laure Avoscan, Jeanine Lherminier, Kangjia Luo, Michel Narce, Jérôme Bellenger

{"title":"The transplantation of the gut microbiome of fat-1 mice protects against colonic mucus layer disruption and endoplasmic reticulum stress induced by high fat diet.","authors":"Amina Bourragat, Quentin Escoula, Sandrine Bellenger, Olivier Zemb, Martin Beaumont, Killian Chaumonnot, Jean-Pierre Farine, Emmanuel Jacotot, Aline Bonnotte, Laure Avoscan, Jeanine Lherminier, Kangjia Luo, Michel Narce, Jérôme Bellenger","doi":"10.1080/19490976.2024.2356270","DOIUrl":"10.1080/19490976.2024.2356270","url":null,"abstract":"High-fat diets alter gut barrier integrity, leading to endotoxemia by impacting epithelial functions and inducing endoplasmic reticulum (ER) stress in intestinal secretory goblet cells. Indeed, ER stress, which is an important contributor to many chronic diseases such as obesity and obesity-related disorders, leads to altered synthesis and secretion of mucins that form the protective mucus barrier. In the present study, we investigated the relative contribution of omega-3 polyunsaturated fatty acid (PUFAs)-modified microbiota to alleviating alterations in intestinal mucus layer thickness and preserving gut barrier integrity. Male fat-1 transgenic mice (exhibiting endogenous omega-3 PUFAs tissue enrichment) and wild-type (WT) littermates were fed either an obesogenic high-fat diet (HFD) or a control diet. Unlike WT mice, HFD-fed fat-1 mice were protected against mucus layer alterations as well as an ER stress-mediated decrease in mucin expression. Moreover, cecal microbiota transferred from fat-1 to WT mice prevented changes in the colonic mucus layer mainly through colonic ER stress downregulation. These findings highlight a novel feature of the preventive effects of omega-3 fatty acids against intestinal permeability in obesity-related conditions.","PeriodicalId":12909,"journal":{"name":"Gut Microbes","volume":"16 1","pages":"2356270"},"PeriodicalIF":12.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11135845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0