Gut Pathogens最新文献

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Dynamic changes in the gut microbiota composition during adalimumab therapy in patients with ulcerative colitis: implications for treatment response prediction and therapeutic targets. 溃疡性结肠炎患者在接受阿达木单抗治疗期间肠道微生物群组成的动态变化:对治疗反应预测和治疗目标的影响。
IF 4.3 3区 医学
Gut Pathogens Pub Date : 2024-08-26 DOI: 10.1186/s13099-024-00637-5
Han Na Oh, Seung Yong Shin, Jong-Hwa Kim, Jihye Baek, Hyo Jong Kim, Kang-Moon Lee, Soo Jung Park, Seok-Young Kim, Hyung-Kyoon Choi, Wonyong Kim, Woo Jun Sul, Chang Hwan Choi
{"title":"Dynamic changes in the gut microbiota composition during adalimumab therapy in patients with ulcerative colitis: implications for treatment response prediction and therapeutic targets.","authors":"Han Na Oh, Seung Yong Shin, Jong-Hwa Kim, Jihye Baek, Hyo Jong Kim, Kang-Moon Lee, Soo Jung Park, Seok-Young Kim, Hyung-Kyoon Choi, Wonyong Kim, Woo Jun Sul, Chang Hwan Choi","doi":"10.1186/s13099-024-00637-5","DOIUrl":"10.1186/s13099-024-00637-5","url":null,"abstract":"<p><strong>Background: </strong>While significant research exists on gut microbiota changes after anti-tumor necrosis factor-alpha (anti TNF-α) therapy for ulcerative colitis, little is known about the longitudinal changes related to the effects of anti TNF-α. This study aimed to investigate the dynamics of gut microbiome changes during anti TNF-α (adalimumab) therapy in patients with ulcerative colitis (UC).</p><p><strong>Results: </strong>The microbiota composition was affected by the disease severity and extent in patients with UC. Regardless of clinical remission status at each time point, patients with UC exhibited microbial community distinctions from healthy controls. Distinct amplicon sequence variants (ASVs) differences were identified throughout the course of Adalimumab (ADA) treatment at each time point. A notable reduction in gut microbiome dissimilarity was observed only in remitters. Remitters demonstrated a decrease in the relative abundances of Burkholderia-Caballeronia-Paraburkholderia and Staphylococcus as the treatment progressed. Additionally, there was an observed increase in the relative abundances of Bifidobacterium and Dorea. Given the distribution of the 48 ASVs with high or low relative abundances in the pre-treatment samples according to clinical remission at week 8, a clinical remission at week 8 with a sensitivity and specificity of 72.4% and 84.3%, respectively, was predicted on the receiver operating characteristic curve (area under the curve, 0.851).</p><p><strong>Conclusions: </strong>The gut microbiota undergoes diverse changes according to the treatment response during ADA treatment. These changes provide insights into predicting treatment responses to ADA and offer new therapeutic targets for UC.</p>","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11346184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142072607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid and specific differentiation of Salmonella enterica serotypes typhi and Paratyphi by multicolor melting curve analysis. 通过多色熔解曲线分析法快速特异性区分伤寒沙门氏菌和副伤寒沙门氏菌血清型。
IF 4.3 3区 医学
Gut Pathogens Pub Date : 2024-08-19 DOI: 10.1186/s13099-024-00636-6
Yixiang Jiang, Min Jiang, Rui Cai, Xiaolu Shi, Qinghua Hu, Biao Kan
{"title":"Rapid and specific differentiation of Salmonella enterica serotypes typhi and Paratyphi by multicolor melting curve analysis.","authors":"Yixiang Jiang, Min Jiang, Rui Cai, Xiaolu Shi, Qinghua Hu, Biao Kan","doi":"10.1186/s13099-024-00636-6","DOIUrl":"10.1186/s13099-024-00636-6","url":null,"abstract":"<p><p>Rapid and accurate identification of Salmonella enterica serotypes Typhi and Paratyphi (A, B and C), the causal agents of enteric fever, is critical for timely treatment, case management and evaluation of health policies in low and middle-income countries where the disease still remains a serious public health problem. The present study describes the development of a multiplex assay (EFMAtyping) for simultaneous identification of pathogens causing typhoid and paratyphoid fever in a single reaction by the MeltArray approach, which could be finished within 2.5 h. Seven specific genes were chosen for differentiation of typhoidal and nontyphoidal Salmonella. All gene targets were able to be detected by the EFMAtyping assay, with expected Tm values and without cross-reactivity to other relevant Salmonella serovars. The limit of detection (LOD) for all gene targets was 50 copies per reaction. The LOD reached 10<sup>2</sup>-10<sup>3</sup> CFU/ml for each pathogen in simulated clinical samples. The largest standard deviation value for mean Tm was below 0.5 °C. This newly developed EFMAtyping assay was further evaluated by testing 551 clinical Salmonella isolates, corroborated in parallel by the traditional Salmonella identification workflow, and serotype prediction was enabled by whole-genome sequencing. Compared to the traditional method, our results exhibited 100% of specificity and greater than 96% of sensitivity with a kappa correlation ranging from 0.96 to 1.00. Thus, the EFMAtyping assay provides a rapid, high throughput, and promising tool for public health laboratories to monitor typhoid and paratyphoid fever.</p>","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11331607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lower fecal microbiota transplantation ameliorates ulcerative colitis by eliminating oral-derived Fusobacterium nucleatum and virulence factor. 下粪便微生物群移植通过消除口源性核酸镰刀菌和毒力因子改善溃疡性结肠炎。
IF 4.3 3区 医学
Gut Pathogens Pub Date : 2024-08-08 DOI: 10.1186/s13099-024-00633-9
Dong-Hao Li, Zong-Wei Li, Qi Sun, Lei Wang, Shou-Bin Ning
{"title":"Lower fecal microbiota transplantation ameliorates ulcerative colitis by eliminating oral-derived Fusobacterium nucleatum and virulence factor.","authors":"Dong-Hao Li, Zong-Wei Li, Qi Sun, Lei Wang, Shou-Bin Ning","doi":"10.1186/s13099-024-00633-9","DOIUrl":"10.1186/s13099-024-00633-9","url":null,"abstract":"<p><strong>Background: </strong>Recently, the oral oncobacterium Fusobacterium nucleatum (F. nucleatum), has been linked with ulcerative colitis (UC). Here, we aim to investigate whether Fecal Microbiota Transplantation (FMT) can alleviate UC by restoring gut microbiota and eliminating oral-derived F. nucleatum and virulence factor fadA.</p><p><strong>Method: </strong>C57BL/6J mice were randomly divided into a healthy control group (HC), Dextran Sulfate Sodium group (DSS), oral inoculation group (OR), upper FMT group (UFMT), and lower FMT group (LFMT). Disease activity index, body weight, survival rate, and histopathological scores were used to measure the severity of colitis. The function of the intestinal mucosal barrier was evaluated by performing immunohistochemical staining of the tight junction protein Occludin. Real-time PCR was used to assess the relative abundance of the nusG gene and the virulence gene fadA. Cytokine levels were detected by ELISA. Full-length sequencing of 16S rRNA was used to analyze the changes and composition of gut microbiota.</p><p><strong>Findings: </strong>Oral incubation of F. nucleatum further exacerbated the severity of colitis and gut dysbiosis. Peptostreptococcaceae, Enterococcaceae, and Escherichia coli were significantly enriched in OR mice. However, LFMT mice showed an obvious decrease in disease activity and were more effective in restoring gut microbiota and eliminating F. nucleatum than UFMT mice. Bacteroidota, Lachnospiraceae, and Prevotellaceae were mainly enriched bacteria in LFMT mice. In addition, Genera such as Lactobacillus, Allobaculum, and Bacteroidales were found negative correlation with TNF-α, IL-1β, and IL-6. Genera like Romboutsia, Escherichia Shigella, Enterococcus, and Clostridium were found positively correlated with TNF-α, IL-1β, and IL-6.</p><p><strong>Conclusions: </strong>Oral incubation of F. nucleatum further exacerbates the severity and dysbiosis in DSS-induced colitis mice. Besides, lower tract FMT can ameliorate colitis by restoring the gut microbiota diversity and eliminating F. nucleatum and virulence factor fadA.</p>","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11311926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The influence of Akkermansia muciniphila on intestinal barrier function Akkermansia muciniphila 对肠道屏障功能的影响
IF 4.2 3区 医学
Gut Pathogens Pub Date : 2024-08-03 DOI: 10.1186/s13099-024-00635-7
Chunyan Mo, Xiran Lou, Jinfang Xue, Zhuange Shi, Yifang Zhao, Fuping Wang, Guobing Chen
{"title":"The influence of Akkermansia muciniphila on intestinal barrier function","authors":"Chunyan Mo, Xiran Lou, Jinfang Xue, Zhuange Shi, Yifang Zhao, Fuping Wang, Guobing Chen","doi":"10.1186/s13099-024-00635-7","DOIUrl":"https://doi.org/10.1186/s13099-024-00635-7","url":null,"abstract":"Intestinal barriers play a crucial role in human physiology, both in homeostatic and pathological conditions. Disruption of the intestinal barrier is a significant factor in the pathogenesis of gastrointestinal inflammatory diseases, such as inflammatory bowel disease. The profound influence of the gut microbiota on intestinal diseases has sparked considerable interest in manipulating it through dietary interventions, probiotics, and fecal microbiota transplantation as potential approaches to enhance the integrity of the intestinal barrier. Numerous studies have underscored the protective effects of specific microbiota and their associated metabolites. In recent years, an increasing body of research has demonstrated that Akkermansia muciniphila (A. muciniphila, Am) plays a beneficial role in various diseases, including diabetes, obesity, aging, cancer, and metabolic syndrome. It is gaining popularity as a regulator that influences the intestinal flora and intestinal barrier and is recognized as a ‘new generation of probiotics’. Consequently, it may represent a potential target and promising therapy option for intestinal diseases. This article systematically summarizes the role of Am in the gut. Specifically, we carefully discuss key scientific issues that need resolution in the future regarding beneficial bacteria represented by Am, which may provide insights for the application of drugs targeting Am in clinical treatment.","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141887251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of capsular polysaccharide phase variation on biofilm formation, motility and gene expression in Vibrio vulnificus. 胶囊多糖相位变化对弧菌生物膜形成、运动和基因表达的影响
IF 4.3 3区 医学
Gut Pathogens Pub Date : 2024-07-29 DOI: 10.1186/s13099-024-00620-0
Tingting Zhang, Shenjie Ji, Miaomiao Zhang, Fei Wu, Xue Li, Xi Luo, Qinglian Huang, Min Li, Yiquan Zhang, Renfei Lu
{"title":"Effect of capsular polysaccharide phase variation on biofilm formation, motility and gene expression in Vibrio vulnificus.","authors":"Tingting Zhang, Shenjie Ji, Miaomiao Zhang, Fei Wu, Xue Li, Xi Luo, Qinglian Huang, Min Li, Yiquan Zhang, Renfei Lu","doi":"10.1186/s13099-024-00620-0","DOIUrl":"10.1186/s13099-024-00620-0","url":null,"abstract":"<p><p>Vibrio vulnificus, a significant marine pathogen, undergoes opaque (Op)-translucent (Tr) colony switching based on whether capsular polysaccharide (CPS) is produced. CPS phase variation is sometime accompanied by genetic variation or down-regulation of particular genes, such as wzb. In addition, CPS prevents biofilm formation and is important to the virulence of V. vulnificus. However, the extent to which there is a difference in gene expression between Tr and Op colonies and the impact of CPS phase variation on other behaviors of V. vulnificus remain unknown. In this work, the data have shown that CPS phase variation of V. vulnificus is affected by incubation time. Tr and Op strains exhibited similar growth rates. However, Tr strains had enhanced biofilm formation capacities but reduced swimming motility compared to Op strains. The RNA-seq assay revealed 488 differentially expressed genes, with 214 downregulated and 274 upregulated genes, between Tr and Op colonies. Genes associated with Tad pili and CPS were downregulated, whereas those involved in flagellum were upregulated, in Tr colonies compared with Op colonies. In addition, 9 putative c-di-GMP metabolism-associated genes and 28 genes encoding putative regulators were significantly differentially expressed, suggesting that CPS phase variation is probably strictly regulated in V. vulnificus. Moreover, 8 genes encoding putative porins were also differentially expressed between the two phenotypic colonies, indicating that bacterial outer membrane was remodeled during CPS phase variation. In brief, this work highlighted the gene expression profiles associated with CPS phase variation, but more studies should be performed to disclose the intrinsic mechanisms in the future.</p>","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11287873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Desulfovibrio vulgaris caused gut inflammation and aggravated DSS-induced colitis in C57BL/6 mice model. 脱硫弧菌会引起肠道炎症,并加重 C57BL/6 小鼠模型中 DSS 诱导的结肠炎。
IF 4.3 3区 医学
Gut Pathogens Pub Date : 2024-07-26 DOI: 10.1186/s13099-024-00632-w
Guoxin Huang, Yilin Zheng, Ni Zhang, Guohai Huang, Weijin Zhang, Qingnan Li, Xuecong Ren
{"title":"Desulfovibrio vulgaris caused gut inflammation and aggravated DSS-induced colitis in C57BL/6 mice model.","authors":"Guoxin Huang, Yilin Zheng, Ni Zhang, Guohai Huang, Weijin Zhang, Qingnan Li, Xuecong Ren","doi":"10.1186/s13099-024-00632-w","DOIUrl":"10.1186/s13099-024-00632-w","url":null,"abstract":"<p><strong>Background: </strong>Sulfate-reducing bacteria (SRB) is a potential pathogen usually detected in patients with gastrointestinal diseases. Hydrogen sulfide (H2S), a metabolic byproduct of SRB, was considered the main causative agent that disrupted the morphology and function of gut epithelial cells. Associated study also showed that flagellin from Desulfovibrio vulgaris (DVF), the representative bacterium of the Desulfovibrio genus, could exacerbate colitis due to the interaction of DVF and LRRC19, leading to the secretion of pro-inflammatory cytokines. However, we still have limited understanding about the change of gut microbiota (GM) composition caused by overgrowth of SRB and its exacerbating effects on colitis.</p><p><strong>Results: </strong>In this study, we transplanted D. vulgaris into the mice treated with or without DSS, and set a one-week recovery period to investigate the impact of D. vulgaris on the mice model. The outcomes showed that transplanted D. vulgaris into the normal mice could cause the gut inflammation, disrupt gut barrier and reduce the level of short-chain fatty acids (SCFAs). Moreover, D. vulgaris also significantly augmented DSS-induced colitis by exacerbating the damage of gut barrier and the secretion of inflammatory cytokines, for instance, IL-1β, iNOS, and TNF-α. Furthermore, results also showed that D. vulgaris could markedly change GM composition, especially decrease the relative abundance of SCFAs-producing bacteria. Additionally, D. vulgaris significantly stimulated the growth of Akkermansia muciniphila probably via its metabolic byproduct, H2S, in vivo.</p><p><strong>Conclusions: </strong>Collectively, this study indicated that transplantation of D. vulgaris could cause gut inflammation and aggravate the colitis induced by DSS.</p>","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11282857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141765838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selective depletion of Campylobacter jejuni via T6SS dependent functionality: an approach for improving chickens gut health. 通过 T6SS 依赖性功能选择性去除空肠弯曲杆菌:改善鸡肠道健康的一种方法。
IF 4.3 3区 医学
Gut Pathogens Pub Date : 2024-07-12 DOI: 10.1186/s13099-024-00628-6
Subhadeep Gupta, Prakash Biswas, Bishnu Das, Samiran Mondal, Parna Gupta, Dipjyoti Das, Amirul Islam Mallick
{"title":"Selective depletion of Campylobacter jejuni via T6SS dependent functionality: an approach for improving chickens gut health.","authors":"Subhadeep Gupta, Prakash Biswas, Bishnu Das, Samiran Mondal, Parna Gupta, Dipjyoti Das, Amirul Islam Mallick","doi":"10.1186/s13099-024-00628-6","DOIUrl":"10.1186/s13099-024-00628-6","url":null,"abstract":"<p><p>The targeted depletion of potential gut pathogens is often challenging because of their intrinsic ability to thrive in harsh gut environments. Earlier, we showed that Campylobacter jejuni (C. jejuni) exclusively uses the Type-VI Secretion System (T6SS) to target its prey such as Escherichia coli (E. coli), and phenotypic differences between T6SS-negative and T6SS-positive C. jejuni isolates toward bile salt sensitivity. However, it remains unclear how the target-driven T6SS functionality prevails in a polymicrobial gut environment. Here, we investigated the fate of microbial competition in an altered gut environment via bacterial T6SS using a T6SS-negative and -positive C. jejuni or its isogenic mutant of the hemolysin-coregulated protein (hcp). We showed that in the presence of bile salt and prey bacteria (E. coli), T6SS-positive C. jejuni experiences enhanced intracellular stress leading to cell death. Intracellular tracking of fluorophore-conjugated bile salts confirmed that T6SS-mediated bile salt influx into C. jejuni can enhance intracellular oxidative stress, affecting C. jejuni viability. We further investigated whether the T6SS activity in the presence of prey (E. coli) perturbs the in vivo colonization of C. jejuni. Using chickens as primary hosts of C. jejuni and non-pathogenic E. coli as prey, we showed a marked reduction of C. jejuni load in chickens cecum when bile salt solution was administered orally. Analysis of local antibody responses and pro-inflammatory gene expression showed a reduced risk of tissue damage, indicating that T6SS activity in the complex gut environment can be exploited as a possible measure to clear the persistent colonization of C. jejuni in chickens.</p>","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11245787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141599109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Network analysis of gut microbial communities reveal key genera for a multiple sclerosis cohort with Mycobacterium avium subspecies paratuberculosis infection. 肠道微生物群落网络分析揭示了感染副结核分枝杆菌亚种的多发性硬化症队列中的关键菌属。
IF 4.3 3区 医学
Gut Pathogens Pub Date : 2024-07-10 DOI: 10.1186/s13099-024-00627-7
Hajra Ashraf, Plamena Dikarlo, Aurora Masia, Ignazio R Zarbo, Paolo Solla, Umer Zeeshan Ijaz, Leonardo A Sechi
{"title":"Network analysis of gut microbial communities reveal key genera for a multiple sclerosis cohort with Mycobacterium avium subspecies paratuberculosis infection.","authors":"Hajra Ashraf, Plamena Dikarlo, Aurora Masia, Ignazio R Zarbo, Paolo Solla, Umer Zeeshan Ijaz, Leonardo A Sechi","doi":"10.1186/s13099-024-00627-7","DOIUrl":"10.1186/s13099-024-00627-7","url":null,"abstract":"<p><strong>Background: </strong>In gut ecosystems, there is a complex interplay of biotic and abiotic interactions that decide the overall fitness of an individual. Divulging the microbe-microbe and microbe-host interactions may lead to better strategies in disease management, as microbes rarely act in isolation. Network inference for microbial communities is often a challenging task limited by both analytical assumptions as well as experimental approaches. Even after the network topologies are obtained, identification of important nodes within the context of underlying disease aetiology remains a convoluted task. We therefore present a network perspective on complex interactions in gut microbial profiles of individuals who have multiple sclerosis with and without Mycobacterium avium subspecies paratuberculosis (MAP) infection. Our exposé is guided by recent advancements in network-wide statistical measures that identify the keystone nodes. We have utilised several centrality measures, including a recently published metric, Integrated View of Influence (IVI), that is robust against biases.</p><p><strong>Results: </strong>The ecological networks were generated on microbial abundance data (n = 69 samples) utilising 16 S rRNA amplification. Using SPIEC-EASI, a sparse inverse covariance estimation approach, we have obtained networks separately for MAP positive (+), MAP negative (-) and healthy controls (as a baseline). Using IVI metric, we identified top 20 keystone nodes and regressed them against covariates of interest using a generalised linear latent variable model. Our analyses suggest Eisenbergiella to be of pivotal importance in MS irrespective of MAP infection. For MAP + cohort, Pyarmidobacter, and Peptoclostridium were predominately the most influential genera, also hinting at an infection model similar to those observed in Inflammatory Bowel Diseases (IBDs). In MAP- cohort, on the other hand, Coprostanoligenes group was the most influential genera that reduces cholesterol and supports the intestinal barrier.</p><p><strong>Conclusions: </strong>The identification of keystone nodes, their co-occurrences, and associations with the exposome (meta data) advances our understanding of biological interactions through which MAP infection shapes the microbiome in MS individuals, suggesting the link to the inflammatory process of IBDs. The associations presented in this study may lead to development of improved diagnostics and effective vaccines for the management of the disease.</p>","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11238521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The C-terminal proline-rich repeats of Enteropathogenic E. coli effector EspF are sufficient for the depletion of tight junction membrane proteins and interactions with early and recycling endosomes. 肠致病性大肠杆菌效应物 EspF 的 C 端富含脯氨酸的重复序列足以消耗紧密连接膜蛋白以及与早期和再循环内体的相互作用。
IF 4.3 3区 医学
Gut Pathogens Pub Date : 2024-07-07 DOI: 10.1186/s13099-024-00626-8
Imran Ansari, Anupam Mandal, Kritika Kansal, Pangertoshi Walling, Sumbul Khan, Saima Aijaz
{"title":"The C-terminal proline-rich repeats of Enteropathogenic E. coli effector EspF are sufficient for the depletion of tight junction membrane proteins and interactions with early and recycling endosomes.","authors":"Imran Ansari, Anupam Mandal, Kritika Kansal, Pangertoshi Walling, Sumbul Khan, Saima Aijaz","doi":"10.1186/s13099-024-00626-8","DOIUrl":"10.1186/s13099-024-00626-8","url":null,"abstract":"<p><strong>Background: </strong>Enteropathogenic E. coli (EPEC) causes acute infantile diarrhea accounting for significant morbidity and mortality in developing countries. EPEC uses a type three secretion system to translocate more than twenty effectors into the host intestinal cells. At least four of these effectors, namely EspF, Map, EspG1/G2 and NleA, are reported to disrupt the intestinal tight junction barrier. We have reported earlier that the expression of EspF and Map in MDCK cells causes the depletion of the TJ membrane proteins and compromises the integrity of the intestinal barrier. In the present study, we have examined the role of the proline-rich repeats (PRRs) within the C-terminus of EspF in the depletion of the tight junction membrane proteins and identified key endocytosis markers that interact with EspF via these repeats.</p><p><strong>Results: </strong>We generated mutant EspF proteins which lacked one or more proline-rich repeats (PRRs) from the N-terminus of EspF and examined the effect of their expression on the cellular localization of tight junction membrane proteins. In lysates derived from cells expressing the mutant EspF proteins, we found that the C-terminal PRRs of EspF are sufficient to cause the depletion of TJ membrane proteins. Pull-down assays revealed that the PRRs mediate interactions with the TJ adaptor proteins ZO-1 and ZO-2 as well as with the proteins involved in endocytosis such as caveolin-1, Rab5A and Rab11.</p><p><strong>Conclusions: </strong>Our study demonstrates the direct role of the proline-rich repeats of EspF in the depletion of the TJ membrane proteins and a possible involvement of the PRRs in the endocytosis of host proteins. New therapeutic strategies can target these PRR domains to prevent intestinal barrier dysfunction in EPEC infections.</p>","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11229284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Equol: a metabolite of gut microbiota with potential antitumor effects. Equol:一种具有潜在抗肿瘤作用的肠道微生物群代谢物。
IF 4.3 3区 医学
Gut Pathogens Pub Date : 2024-07-07 DOI: 10.1186/s13099-024-00625-9
Jing Lv, Shengkai Jin, Yuwei Zhang, Yuhua Zhou, Menglu Li, Ninghan Feng
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