Chicken as a carrier of emerging virulent Helicobacter species: a potential zoonotic risk.

IF 4.3 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Gut Pathogens Pub Date : 2025-05-21 DOI:10.1186/s13099-025-00707-2

Ahmed Samir, Hala M Zaher

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引用次数: 0

Abstract

Background: The research scope regarding Helicobacter species in chickens, other than H. pullorum, is largely overlooked. This study aimed to investigate the prevalence of emerging Helicobacter species in chickens and the occurrence of the virulence gene cytolethal distending toxin B (cdtB) among the identified Helicobacter species, referring to their public health significance.

Methods: A total of 390 cloacal swabs were collected from 205 broilers and 185 layers. The swabs were pooled into 78 pools. DNA was extracted from these pools, followed by Helicobacter 16S rRNA gene PCR. Twenty pools positive for Helicobacter 16S rRNA were analyzed for H. pylori and H. pullorum, then Helicobacter 16S rRNA sequencing was performed on ten negative pools for H. pullorum and H. pylori to identify Helicobacter species. Subsequently, cdtB was investigated in the 20 pools positive for Helicobacter. Following that, partial DNA sequencing of one H. pullorum and one H. brantae cdtB gene from broiler and layer chickens, respectively, was carried out.

Results: Overall, 25.6% of the examined pools were positive for Helicobacter spp., with 3 (7.3%) and 17 (45.9%) broiler and layer pools being positive, respectively. All three broiler pools were identified as H. pullorum; seven-layer pools were positive for H. pullorum, while H. pylori could not be detected. Helicobacter 16S rRNA sequencing of ten negative layer pools for H. pullorum and H. pylori revealed 6 H. brantae, 2 H. kayseriensis, 1 H. winghamensis, and 1 Helicobacter sp. Tul. The cdtB gene was found in 10 H. pullorum, 5 H. brantae, 1 H. winghamensis, and 1 Helicobacter sp. Tul. Phylogenetic analysis of Helicobacter 16S rRNA sequences and BLAST analysis of H. pullorum and H. brantae cdtB partial sequences underscore the public health importance of the obtained sequences.

Conclusion: This study highlights that the occurrence of emerging virulent Helicobacter species in chicken feces poses a potential zoonotic relevance and public health risk.

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鸡作为新出现的有毒幽门螺杆菌的携带者：潜在的人畜共患风险。

背景：鸡体内除白痢螺杆菌外的其他螺杆菌种类的研究范围在很大程度上被忽视了。本研究旨在了解新出现的幽门螺杆菌种属在鸡群中的流行情况，以及所鉴定的幽门螺杆菌种属中毒力基因细胞致死膨胀毒素B （cdtB）的发生情况，以探讨其公共卫生意义。方法：收集205只肉鸡、185只蛋鸡的粪拭子390份。将拭子放入78个池中。从这些池中提取DNA，然后进行16S rRNA基因PCR。对20个幽门螺杆菌16S rRNA阳性池进行幽门螺杆菌和幽门螺杆菌分析，对10个幽门螺杆菌和幽门螺杆菌阴性池进行16S rRNA测序，鉴定幽门螺杆菌种类。随后，在幽门螺杆菌阳性的20个池中进行了cdtB调查。随后，分别对肉仔鸡和蛋鸡的1个白痢嗜血杆菌和1个brantae嗜血杆菌cdtB基因进行了部分DNA测序。结果：25.6%的肉鸡池和蛋鸡池幽门螺杆菌阳性，分别为3个（7.3%）和17个（45.9%）。所有3个肉鸡池均被鉴定为白痢杆菌；7层池中检出白痢杆菌阳性，幽门螺旋杆菌未检出。对10个幽门螺杆菌和黄螺杆菌阴性层库进行16S rRNA测序，结果显示brantae H. 6, kayseriensis H. 2, winghamensis H. 1, Helicobacter sp. Tul. 1。cdtB基因在10株白痢H.、5株brantae H.、1株winghamensis H.和1株幽门螺杆菌中发现。对幽门螺杆菌16S rRNA序列的系统发育分析以及H. pullorum和H. brantae cdtB部分序列的BLAST分析强调了所获得的序列对公共卫生的重要性。结论：鸡粪便中新出现的毒力幽门螺杆菌具有潜在的人畜共患相关性和公共卫生风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gut Pathogens GASTROENTEROLOGY & HEPATOLOGY-MICROBIOLOGY

CiteScore

7.70

自引率

2.40%

发文量

期刊介绍： Gut Pathogens is a fast publishing, inclusive and prominent international journal which recognizes the need for a publishing platform uniquely tailored to reflect the full breadth of research in the biology and medicine of pathogens, commensals and functional microbiota of the gut. The journal publishes basic, clinical and cutting-edge research on all aspects of the above mentioned organisms including probiotic bacteria and yeasts and their products. The scope also covers the related ecology, molecular genetics, physiology and epidemiology of these microbes. The journal actively invites timely reports on the novel aspects of genomics, metagenomics, microbiota profiling and systems biology. Gut Pathogens will also consider, at the discretion of the editors, descriptive studies identifying a new genome sequence of a gut microbe or a series of related microbes (such as those obtained from new hosts, niches, settings, outbreaks and epidemics) and those obtained from single or multiple hosts at one or different time points (chronological evolution).