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The effects of 12 weeks of high-intensity interval training and moderate-intensity continuous training on FGF21, irisin, and myostatin in men with type 2 diabetes mellitus. 12周高强度间歇训练和中等强度连续训练对2型糖尿病男性FGF21、鸢尾素和肌肉生长抑制素的影响。
IF 1.8 4区 生物学
Growth factors Pub Date : 2024-02-01 Epub Date: 2024-01-31 DOI: 10.1080/08977194.2023.2279163
Simin Riahy
{"title":"The effects of 12 weeks of high-intensity interval training and moderate-intensity continuous training on FGF21, irisin, and myostatin in men with type 2 diabetes mellitus.","authors":"Simin Riahy","doi":"10.1080/08977194.2023.2279163","DOIUrl":"10.1080/08977194.2023.2279163","url":null,"abstract":"<p><p>This study investigated the influence of a 12-week high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on irisin, fibroblast growth factor 21 (FGF21), and myostatin (MSTN) among men with type 2 diabetes mellitus (T2DM). Forty-five adult men with T2DM were randomly selected and assigned to receive and perform HIIT (4 × 4 min at 85-95% HRmax with three min of active rest at 50-60% HRmax in between) and MICT (walking/running continuously for 47 min at 60-70% HRmax) three sessions per week for 12 weeks, or to act as a non-exercise control (CON) group. The subjects' blood samples were collected at baseline and 48 hours after the last intervention session. Our research revealed that both interventions resulted in similar decreases in FGF21 and MSTN when compared to the CON (<i>p</i> < .01). However, only the HIIT group showed a significant increase in irisin (<i>p</i> < .01) compared to the CON. Further, improvements in insulin resistance, body composition, and VO<sub>2</sub> peak were noted in both intervention groups compared with those of the CON group (<i>p</i> < .01). It seems that while either aerobic exercise strategy could be seen as a therapy for men with T2DM, HIIT had a more advantageous effect on the irisin response.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"24-35"},"PeriodicalIF":1.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72014130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dynamics of transforming growth factor β signaling and therapeutic efficacy. 转化生长因子β信号转导的动态及治疗效果。
IF 1.8 4区 生物学
Growth factors Pub Date : 2023-10-01 Epub Date: 2023-05-25 DOI: 10.1080/08977194.2023.2215335
Siqi Wu, Rodney Brian Luwor, Hong-Jian Zhu
{"title":"Dynamics of transforming growth factor β signaling and therapeutic efficacy.","authors":"Siqi Wu, Rodney Brian Luwor, Hong-Jian Zhu","doi":"10.1080/08977194.2023.2215335","DOIUrl":"10.1080/08977194.2023.2215335","url":null,"abstract":"<p><p>Transforming growth factor β (TGFβ) is a multifunctional cytokine, and its signalling responses are exerted via integrated intracellular pathways and complex regulatory mechanisms. Due to its high potency, TGFβ signalling is tightly controlled under normal circumstances, while its dysregulation in cancer favours metastasis. The recognised potential of TGFβ as a therapeutic target led to emerging development of anti-TGFβ reagents with preclinical success, yet these therapeutics failed to recapitulate their efficacy in experimental settings. In this review, possible reasons for this inconsistency are discussed, addressing the knowledge gap between theoretical and actual behaviours of TGFβ signalling. Previous studies on oncogenic cells have demonstrated the spatiotemporal heterogeneity of TGFβ signalling intensity. Under feedback mechanisms and exosomal ligand recycling, cancer cells may achieve cyclic TGFβ signalling to facilitate dissemination and colonisation. This challenges the current presumption of persistently high TGFβ signalling in cancer, pointing to a new direction of research on TGFβ-targeted therapeutics.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":"41 2","pages":"82-100"},"PeriodicalIF":1.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9732296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Technological advances define shifting pathway signaling from normal to primary and metastatic colorectal cancer. 技术进步定义了从正常到原发性和转移性癌症的转移途径信号。
IF 1.8 4区 生物学
Growth factors Pub Date : 2023-10-01 Epub Date: 2023-06-23 DOI: 10.1080/08977194.2023.2227274
Robert G Ramsay, Vicki Whitehall, Michael P Flood
{"title":"Technological advances define shifting pathway signaling from normal to primary and metastatic colorectal cancer.","authors":"Robert G Ramsay,&nbsp;Vicki Whitehall,&nbsp;Michael P Flood","doi":"10.1080/08977194.2023.2227274","DOIUrl":"10.1080/08977194.2023.2227274","url":null,"abstract":"<p><p>Adoption of organoid/tumoroid propagation of normal and malignant intestinal epithelia has provided unparalleled opportunities to compare cell growth factor and signaling dependencies. These 3D structures recapitulate tumours in terms of gene expression regarding the tumor cells but also allow deeper insights into the contribution of the tumour microenvironment (TME). Elements of the TME can be manipulated or added back in the form of infiltrating cytotoxic lymphocytes and/or cancer associated fibroblasts. The effectiveness of chemo-, radio- and immunotherapies can be explored within weeks of deriving these patient-derived tumour avatars informing treatment of these exact patients in a timely manner. Entrenched paths to colorectal cancer (CRC) from the earliest steps of conventional adenoma or serrated lesion formation, and the recognition of further sub-categorisations embodied by consensus-molecular-subtypes (CMS), provide genetic maps allowing a molecular form of pathologic taxonomy. Recent advances in organoid propagation and scRNAseq are reshaping our understanding of CMS and CRC.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"179-191"},"PeriodicalIF":1.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9676497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A review about the role of additives in nerve tissue engineering: growth factors, vitamins, and drugs. 添加剂在神经组织工程中的作用综述：生长因子、维生素和药物。
IF 1.8 4区 生物学
Growth factors Pub Date : 2023-10-01 Epub Date: 2023-06-21 DOI: 10.1080/08977194.2023.2226938
Mehrsa Nasiri, Javad Esmaeili, Amir Tebyani, Hojat Basati
{"title":"A review about the role of additives in nerve tissue engineering: growth factors, vitamins, and drugs.","authors":"Mehrsa Nasiri, Javad Esmaeili, Amir Tebyani, Hojat Basati","doi":"10.1080/08977194.2023.2226938","DOIUrl":"10.1080/08977194.2023.2226938","url":null,"abstract":"<p><p>Notably the integration of additives such as growth factors, vitamins, and drugs with scaffolds promoted nerve tissue engineering. This study tried to provide a concise review of all these additives that facilitates nerve regeneration. An attempt was first made to provide information on the main principle of nerve tissue engineering, and then to shed light on the effectiveness of these additives on nerve tissue engineering. Our research has shown that growth factors accelerate cell proliferation and survival, while vitamins play an effective role in cell signalling, differentiation, and tissue growth. They can also act as hormones, antioxidants, and mediators. Drugs also have an excellent and necessary effect on this process by reducing inflammation and immune responses. This review shows that growth factors were more effective than vitamins and drugs in nerve tissue engineering. Nevertheless, vitamins were the most commonly used additive in the production of nerve tissue.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":"41 2","pages":"101-113"},"PeriodicalIF":1.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9790921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcription factors TP63 facilitates malignant progression of thyroid cancer by upregulating KRT17 expression and inducing epithelial-mesenchymal transition. 转录因子TP63通过上调KRT17表达，诱导上皮-间质转化，促进甲状腺癌的恶性进展。
IF 1.8 4区 生物学
Growth factors Pub Date : 2023-10-01 Epub Date: 2023-03-15 DOI: 10.1080/08977194.2023.2184656
Fanbo Meng, Liting Dai
{"title":"Transcription factors TP63 facilitates malignant progression of thyroid cancer by upregulating KRT17 expression and inducing epithelial-mesenchymal transition.","authors":"Fanbo Meng, Liting Dai","doi":"10.1080/08977194.2023.2184656","DOIUrl":"10.1080/08977194.2023.2184656","url":null,"abstract":"<p><p>Thyroid cancer (TC) is a relatively prevalent endocrine tumor among women, the incidence of which is rapidly rising. In this present study, we aimed to provide new therapeutic targets from the aspect of transcription factor-target gene interaction. TP63 and KRT17 were both highly expressed in TC tissues and cells. The results of ChIP and dual-luciferase assays confirmed TP63 to bind the KRT17 promoter. Cell function assays revealed that knockdown of TP63 could repress TC cell progression. Furthermore, the rescue assay verified that TP63 could facilitate KRT17 expression to activate the AKT signaling pathway, which in turn stimulated TC cell invasion and migration, and induced EMT. All these results verified that TP63 facilitates TC malignant progression by promoting KRT17 expression and inducing EMT.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":"41 2","pages":"71-81"},"PeriodicalIF":1.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9809436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterisation of mesenchymal stem cells conditioned media obtained at different conditioning times: their effect on glial cells in in vitro scratch model. 不同条件下获得的间充质干细胞条件培养基的特性及其对体外划痕模型中胶质细胞的影响。
IF 1.8 4区 生物学
Growth factors Pub Date : 2023-10-01 Epub Date: 2023-02-24 DOI: 10.1080/08977194.2023.2182145
Eva Székiová, Zuzana Michalová, Juraj Blaško, Rastislav Mucha, Lucia Slovinská, Martin Kello, Ivo Vanický
{"title":"Characterisation of mesenchymal stem cells conditioned media obtained at different conditioning times: their effect on glial cells in <i>in vitro</i> scratch model.","authors":"Eva Székiová, Zuzana Michalová, Juraj Blaško, Rastislav Mucha, Lucia Slovinská, Martin Kello, Ivo Vanický","doi":"10.1080/08977194.2023.2182145","DOIUrl":"10.1080/08977194.2023.2182145","url":null,"abstract":"<p><p>In this study, the bone marrow mesenchymal stem cells conditioned media (BMMSC-CM) obtained by conditioning for 24(CM24), 48(CM48) and 72(CM72) hours was characterised. In vitro, the impact of BMMSC-CM on the astrocyte migratory response and oligodendrocyte density was evaluated using the scratch model. The proteomic profiles of individual secretomes were analysed by mass spectrometry and the concentrations of four selected neurotrophins (BDNF, NGF, GDNF and VEGF) were determined by ELISA. Our results revealed an increased number of proteins at CM72, many of which are involved in neuroregenerative processes. ELISA documented a gradual increase in the concentration of two neurotrophins (NGF, VEGF), peaking at CM72. <i>In vitro</i>, the different effect of individual BMMSC-CM on astrocyte migration response and oligodendrocyte density was observed, most pronounced with CM72. The outcomes demonstrate that the prolonged conditioning results in increased release of detectable proteins, neurotrophic factors concentration and stronger effect on reparative processes in neural cell cultures.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":"41 2","pages":"57-70"},"PeriodicalIF":1.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10092369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Knock-down of IGFBP2 ameliorates lung fibrosis and inflammation in rats with severe pneumonia through STAT3 pathway. IGFBP2的敲除通过STAT3途径改善严重肺炎大鼠的肺纤维化和炎症。
IF 1.8 4区 生物学
Growth factors Pub Date : 2023-10-01 Epub Date: 2023-10-19 DOI: 10.1080/08977194.2023.2259497
Yuyu Wang, Jianjiang Huang, Fang Zhang, Keli Shen, Bin Qiu
{"title":"Knock-down of IGFBP2 ameliorates lung fibrosis and inflammation in rats with severe pneumonia through STAT3 pathway.","authors":"Yuyu Wang,&nbsp;Jianjiang Huang,&nbsp;Fang Zhang,&nbsp;Keli Shen,&nbsp;Bin Qiu","doi":"10.1080/08977194.2023.2259497","DOIUrl":"10.1080/08977194.2023.2259497","url":null,"abstract":"<p><strong>Objective: </strong>To observe the mechanism of IGFBP2 knock-down in improving lung fibrosis and inflammation through STAT3 pathway in rats with severe pneumonia.</p><p><strong>Materials and methods: </strong>First, SP rat model was established. Then rats were divided into the Control group, the SP group, the SP + Lv-vector shRNA group, the SP + Lv-IGFBP2 shRNA group, the SP + Lv-vector group, and the SP + Lv-IGFBP2 group. The mRNA and protein levels of IGFBP2, NOS, CD206 and Arg 1 were detected by RT-qPCR and Western blot. IHC was used to check the positive expression of IGFBP2 and MCP1. A fully automated blood gas analyzer was used to detected PaCO<sub>2</sub>, CO<sub>2</sub> content, PaO2 and SaO2. HE and Masson staining were performed to observe the lung tissue injury and collagen deposition of rats in each group. ELISA assays were used to calculate the levels of inflammatory factors IL-1β, IL-6, TNF-α, IL-4, and IL-10. Flow cytometry was conducted to acquire the ratio of M1-type AMs and M2-type AMs.</p><p><strong>Results: </strong>Compared with the Control group, IGFBP2, iNOS, CD206, and Arg1 mRNA and protein expression levels, IGFBP2 and MCP1 positive expressions, PaCO<sub>2</sub>, p-STAT3/STAT3, p-JAK2/JAK2, IL-1β, IL-6, and TNF-α levels, the number of AMs and neutrophils, the proportion of M1 type AMs and the expressions of α-SMA, Collagen-I, Collagen III, and Fibronectin were significantly increased in SP rats <i>(p</i> < 0.05), while PaCO<sub>2</sub>, CO<sub>2</sub>, and SaO<sub>2</sub>, IL-4 and IL-10 levels, and the proportion of M2 type AMs decreased (<i>p</i> < 0.05). However, the knockdown of IGFBP2 reversed the above index trends.</p><p><strong>Conclusion: </strong>Knock-down of IGFBP2 ameliorated lung injury in SP rats, inhibited inflammation and pulmonary fibrosis, and promoted M2-type transformation of AMs by activating the STAT3 pathway.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"210-220"},"PeriodicalIF":1.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41095376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of transforming growth factor-β in airway remodelling in bronchiolitis obliterans. 转化生长因子-β在闭塞性细支气管炎气道重塑中的作用。
IF 1.8 4区 生物学
Growth factors Pub Date : 2023-10-01 Epub Date: 2023-07-24 DOI: 10.1080/08977194.2023.2239356
Ziwei Wang
{"title":"Role of transforming growth factor-β in airway remodelling in bronchiolitis obliterans.","authors":"Ziwei Wang","doi":"10.1080/08977194.2023.2239356","DOIUrl":"10.1080/08977194.2023.2239356","url":null,"abstract":"<p><p>Airway remodelling is the main pathological mechanism of bronchiolitis obliterans (BO). Several studies have found that transforming growth factor-β (TGF-β) expression is increased in BO during airway remodelling, where it plays an important role in various biological processes by binding to its receptor complex to activate multiple signalling proteins and pathways. This review examines the role of TGF-β in airway remodelling in BO and its potential as a therapeutic target, highlighting the mechanisms of TGF-β activation and signalling, cellular targets of TGF-β actions, and research progress in TGF-β signalling and TGF-β-mediated processes.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"192-209"},"PeriodicalIF":1.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10242088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative evaluation of autologous platelet-rich plasma and platelet lysate in patients with knee osteoarthritis. 膝关节骨性关节炎患者自体富血小板血浆与血小板裂解液的比较评价。
IF 1.8 4区 生物学
Growth factors Pub Date : 2023-08-01 DOI: 10.1080/08977194.2023.2227273
Sareh Hosseini, Mohammad Sadegh Soltani-Zangbar, Majid Zamani, Yoda Yaghoubi, Reza Rikhtegar Ghiasi, Roza Motavalli, Ali Ghassabi, Rahim Iranzad, Amir Mehdizadeh, Seyed Kazem Shakouri, Alireza Pishgahi, Mehdi Yousefi
{"title":"Comparative evaluation of autologous platelet-rich plasma and platelet lysate in patients with knee osteoarthritis.","authors":"Sareh Hosseini,&nbsp;Mohammad Sadegh Soltani-Zangbar,&nbsp;Majid Zamani,&nbsp;Yoda Yaghoubi,&nbsp;Reza Rikhtegar Ghiasi,&nbsp;Roza Motavalli,&nbsp;Ali Ghassabi,&nbsp;Rahim Iranzad,&nbsp;Amir Mehdizadeh,&nbsp;Seyed Kazem Shakouri,&nbsp;Alireza Pishgahi,&nbsp;Mehdi Yousefi","doi":"10.1080/08977194.2023.2227273","DOIUrl":"https://doi.org/10.1080/08977194.2023.2227273","url":null,"abstract":"<p><p>Autologous platelet-rich plasma (PRP) and platelet lysate (PL) are nowadays promising candidates in the treatment of articular cartilage lesions. We aimed to compare PRP and PL injection effectiveness in patients with knee osteoarthritis (KOA). A total of fifty women with KOA were included in the study. Patients were treated with intra-articular injections of PRP and PL. Clinical outcomes were evaluated using the comparison of VAS, WOMAC, and ROM scores. The concentration levels of growth factors and cytokines were measured by ELISA. All patients showed significant improvements in pain and function following treatment of KOA with PL and PRP compared to baseline. Moreover, PL's concentration of growth factors was significantly higher than PRP. A significant increase was also observed in all of the aforementioned mediators in both PRP and PL products compared to control. These results can introduce PL as a promising and alternative option for KOA therapy in the future.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":"41 3","pages":"165-177"},"PeriodicalIF":1.8,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9904259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcription factor ETV1-induced lncRNA MAFG-AS1 promotes migration, invasion, and epithelial-mesenchymal transition of pancreatic cancer cells by recruiting IGF2BP2 to stabilize ETV1 expression. 转录因子ETV1诱导的lncRNA MAFG-AS1通过招募IGF2BP2来稳定ETV1的表达，从而促进胰腺癌细胞的迁移、侵袭和上皮-间质转化。
IF 1.8 4区 生物学
Growth factors Pub Date : 2023-08-01 Epub Date: 2023-07-10 DOI: 10.1080/08977194.2023.2227272
Hanqin Weng, Weijian Feng, Fengling Li, Dong Huang, Liangyi Lin, Zaiguo Wang
{"title":"Transcription factor ETV1-induced lncRNA MAFG-AS1 promotes migration, invasion, and epithelial-mesenchymal transition of pancreatic cancer cells by recruiting IGF2BP2 to stabilize ETV1 expression.","authors":"Hanqin Weng, Weijian Feng, Fengling Li, Dong Huang, Liangyi Lin, Zaiguo Wang","doi":"10.1080/08977194.2023.2227272","DOIUrl":"10.1080/08977194.2023.2227272","url":null,"abstract":"<p><p>We investigated the mechanism of ETS-translocation variant 1 (ETV1)/lncRNA-MAFG-AS1 in pancreatic cancer (PC). MAFG-AS1 and ETV1 levels in PC cell lines and HPNE cells were determined using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blotting (WB). After transfection with sh-MAFG-AS1, PC cell invasion, migration, proliferation, and epithelial-mesenchymal transition (EMT)-related proteins were measured by 5-ethynyl-2'-deoxyuridine (EdU), Transwell assay, and WB. The binding between ETV1 and MAFG-AS1 was studied using dual-luciferase assay and chromatin immunoprecipitation. The interactions between MAFG-AS1, IGF2BP2, and ETV1 were tested. Combined experiments were further performed using sh-MAFG-AS1 and pcDNA-ETV1 simultaneously. ETV1/MAFG-AS1 was highly expressed in PC cells. Blocking MAFG-AS1 inhibited the malignant behaviors of PC cells. ETV1 induced MAFG-AS1 transcription in PC cells. MAFG-AS1 stabilized ETV1 mRNA by recruiting IGF2BP2. ETV1 overexpression partially antagonized the suppression of silencing MAFG-AS1 on PC cells. ETV1-induced MAFG-AS1 stabilized the ETV1 expression by recruiting IGF2BP2 and promoted PC cell migration, invasion, proliferation, and EMT.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":"41 3","pages":"152-164"},"PeriodicalIF":1.8,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9892950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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