Growth factors最新文献_第4页

MiR-200c regulates invasion, proliferation and EMT of anaplastic thyroid cancer cells by targeting parathyroid hormone like hormone. MiR-200c通过靶向甲状旁腺激素样激素调控间变性甲状腺癌细胞的侵袭、增殖和EMT。

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2108809

Yan Zhang, Yuanyuan Duan, Chenguang Wu, Wen Peng, Wenyu Chen, Li Wang, Zhaoqun Deng

{"title":"MiR-200c regulates invasion, proliferation and EMT of anaplastic thyroid cancer cells by targeting parathyroid hormone like hormone.","authors":"Yan Zhang, Yuanyuan Duan, Chenguang Wu, Wen Peng, Wenyu Chen, Li Wang, Zhaoqun Deng","doi":"10.1080/08977194.2022.2108809","DOIUrl":"https://doi.org/10.1080/08977194.2022.2108809","url":null,"abstract":"This study aimed to explore the specific effect of miR-200c in anaplastic thyroid cancer (ATC). Hth74 and ARO cell lines were used. Proliferation, invasion, and colony formation activities of Hth74 and ARO cell lines affected by miR-200c were studied. Expression of epithelial-to-mesenchymal transition (EMT) markers (E-cadherin, N-cadherin, Slug, and Snail) in the Hth74 and ARO cell lines were validated by western blot and qRT-PCR. In addition, the regulation of the parathyroid hormone-like hormone (PTHLH) by miR-200c was assessed. Overexpression of miR-200c inhibited the invasion, proliferation, and colony formation of the ATC cell lines, whereas its downregulation achieved the opposite results. PTHLH was found to be regulated negatively by miR-200c through a miR-200c binding site within the 3'-UTR of PTHLH. miR-200c repressed the proliferation, invasion, and EMT process of cells in ATC cell lines by targeting PTHLH post-transcriptionally, which indicates that miR-200c may be a potential target for the treatment of ATC.","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10803226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased circulating TGF-β1 is associated with impairment in NK cell effector functions in metastatic melanoma patients. 在转移性黑色素瘤患者中，循环TGF-β1升高与NK细胞效应物功能受损相关。

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2124915

Katarina Mirjačić Martinović, Ana Vuletić, Emina Mališić, Tatjana Srdić-Rajić, Nevena Tišma Miletić, Nada Babović, Vladimir Jurišić

{"title":"Increased circulating TGF-β1 is associated with impairment in NK cell effector functions in metastatic melanoma patients.","authors":"Katarina Mirjačić Martinović, Ana Vuletić, Emina Mališić, Tatjana Srdić-Rajić, Nevena Tišma Miletić, Nada Babović, Vladimir Jurišić","doi":"10.1080/08977194.2022.2124915","DOIUrl":"https://doi.org/10.1080/08977194.2022.2124915","url":null,"abstract":"Transforming growth factor beta (TGF-β) plays a complex role in carcinogenesis. In 30 melanoma patients and 20 healthy controls (HC) we analysed functional and phenotypic characteristics of NK cells by Flow cytometry, gene expression of TGF-β1 in peripheral blood mononuclear cells by qPCR and serum and supernatant level of free TGF-β1 by ELISA. Melanoma patients had significantly higher serum level of circulatingTGF-β1 compared to HC, especially those with metastasis into the central nervous system (subclass M1d) and high LDH serum values. Melanoma patients compared to HC had significantly higher level of TGF-β1 gene in PBMC. TGF-β1 serum values negatively correlate with NK cell activity analysed by CD107a (degranulation marker), IFN-γ, NKG2D, and NKp46 in patients. Study shows the association of high level of TGF-β1 with NK cell inhibition in patients represents the main mechanism of tumour immune evasion. Targeting TGF-β may become an important cancer treatment for improving antitumor immunity.","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9344848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Effects of penehyclidine hydrochloride on myocardial ischaemia-reperfusion injury in rats by inhibiting TLR4/MyD88/NF-κB pathway via miR-199a-3p. 盐酸戊乙奎醚通过miR-199a-3p抑制TLR4/MyD88/NF-κB通路对大鼠心肌缺血再灌注损伤的影响

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2109469

Ling Qiang Bai, Bin Zhe Wang, Qi Wei Liu, Wen Qiang Li, Hang Zhou, Xiao Yan Yang

{"title":"Effects of penehyclidine hydrochloride on myocardial ischaemia-reperfusion injury in rats by inhibiting TLR4/MyD88/NF-κB pathway via miR-199a-3p.","authors":"Ling Qiang Bai, Bin Zhe Wang, Qi Wei Liu, Wen Qiang Li, Hang Zhou, Xiao Yan Yang","doi":"10.1080/08977194.2022.2109469","DOIUrl":"https://doi.org/10.1080/08977194.2022.2109469","url":null,"abstract":"This study was to probe the role of penehyclidine hydrochloride (PHC) mediating the impact of toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-kappa B (NF-κB) signalling pathway on myocardial ischaemia-reperfusion injury (MI/RI) in rats through miR-199a-3p. The rat MI/RI model was established through ligating left anterior descending (LAD) coronary artery. PHC was injected preoperatively into the model rats, and injected with miR-199a-3p lentiviral vector or TLR4 antagonist (TAK-242). Next, cardiac function of rats was examined by echocardiography, and rat serum indicators, oxidative stress levels and inflammatory factors were detected. HE staining was applied to detect pathological tissue structure, TUNEL staining to detect apoptosis rate, qRCR and western blot to detect miR-199a-3p and TLR4/MyD88/NF-κB expressions in rat myocardial tissues. Dual luciferase reporter experiment was conducted to confirm the relationship between miR-199a-3p and TLR4. In conclusion, PHC suppresses TLR4/MyD88/NF-κB signalling pathway through miR-199a-3p, thereby improving MI/RI in rats.","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10797623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

High mobility group box 1 and Dickkopf-related protein 1 as biomarkers of glucose toxicity, atherogenicity, and lower β cell function in patients with type 2 diabetes mellitus. 高迁移率组盒1和dickkopf相关蛋白1作为2型糖尿病患者葡萄糖毒性、动脉粥样硬化性和低β细胞功能的生物标志物

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2126317

Hussein Kadhem Al-Hakeim, Qasim Jasim Al-Kaabi, Michael Maes

引用次数: 3

Matrine induces hepatocellular carcinoma apoptosis and represses EMT and stemness through microRNA-299-3p/PGAM1 axis. 苦参碱通过microRNA-299-3p/PGAM1轴诱导肝癌细胞凋亡，抑制EMT和干细胞。

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2113073

BaoLin Wang, HuiHai Wang, Qin Zhao, Fei Lu, ZhenZhuang Yan, Fang Zhou, QingLun Su

{"title":"Matrine induces hepatocellular carcinoma apoptosis and represses EMT and stemness through microRNA-299-3p/PGAM1 axis.","authors":"BaoLin Wang, HuiHai Wang, Qin Zhao, Fei Lu, ZhenZhuang Yan, Fang Zhou, QingLun Su","doi":"10.1080/08977194.2022.2113073","DOIUrl":"https://doi.org/10.1080/08977194.2022.2113073","url":null,"abstract":"This study explored the impacts of matrine on hepatocellular carcinoma (HCC) cell growth, metastasis, epithelial-mesenchymal transition (EMT), and stemness through regulating the microRNA (miR)-299-3p/phosphoglycerate mutase 1 (PGAM1) axis. The association between miR-299-3p expression with the prognosis of HCC patients was studied. miR-299-3p and PGAM1 sequences were transfected into matrine-treated HCC cells, and cell proliferation, invasion, apoptosis, and stemness were detected, as well as protein expression of EMT- and stemness-related makers. The targeting relationship between miR-299-3p and PGAM1 was identified. Matrine elevated miR-299-3p expression, repressed proliferation, invasion, and anti-apoptosis of HCC cells, and constrained EMT and stemness in vitro. PGAM1 was a target of miR-299-3p. Repression of PGAM1 rescued the effects of miR-299-3p downregulation on HCC cells. Matrine stimulates HCC cell apoptosis and represses the process of EMT and stemness through the miR-299-3p/PGAM1 axis.","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10793795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Halofuginone inhibits cell proliferation and AKT/mTORC1 signaling in uterine leiomyoma cells. Halofuginone抑制子宫平滑肌瘤细胞增殖和AKT/mTORC1信号通路。

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2113394

Jing Lin, Xiaochun Wu

引用次数: 0

Embryonic growth retardation and altered expression of IGF-II is reciprocal induced by phytocompounds during early gestation in mice. 胚胎生长迟缓和IGF-II的表达改变是由植物化合物在小鼠妊娠早期相互诱导的。

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2129018

Khamhee Wangsa, Krishnakshi Misra, Upasa Gowala, Indira Sarma, Purba Jyoti Saikia, Hirendra Nath Sarma

引用次数: 1

Effects of dual-gene modification on biological characteristics of vascular endothelial cells and their significance as reserving cells for chronic wound repair. 双基因修饰对血管内皮细胞生物学特性的影响及其作为慢性创伤修复储备细胞的意义。

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2118119

Lingli Guo, Baohua Wei, Feng Pan, Hasi Wulan, Mi Cai

{"title":"Effects of dual-gene modification on biological characteristics of vascular endothelial cells and their significance as reserving cells for chronic wound repair.","authors":"Lingli Guo, Baohua Wei, Feng Pan, Hasi Wulan, Mi Cai","doi":"10.1080/08977194.2022.2118119","DOIUrl":"https://doi.org/10.1080/08977194.2022.2118119","url":null,"abstract":"bFGF is a commonly used and reliable factor for improving chronic wound healing, and hSulf-1 expression is abundant in surrounding cells of chronic wound tissue and vascular endothelial cells, which can reverse the effect of bFGF and inhibit the signalling activity of cell proliferation. In this study, an adenovirus, Ad5F35ET1-bFGF-shSulf1, was designed for establishing the dual-gene modified vascular endothelial cells, which were used as the repair cells for skin chronic wound. Ad5F35ET1-bFGF-shSulf1 infected ECV304 cells in vitro and mediated the overexpression of bFGF and the knockdown of hSulf-1, which effectively activated the AKT and ERK signal transduction pathways, facilitate cell proliferation and migration, with the cell viability to 128.29% at 72 h after infection, compared to 66.65%, 73.74%, 87.63%, 103.14% in the blank control, Ad5F35ET1-EGFP-shNC, Ad5F35ET1-shSulf1, Ad5F35ET1-bFGF groups, respectively. In the rat ear skin injury model, the wound healing was significantly accelerated in the Ad5F35ET1-rbFGF-shrSulf1 group compared to the blank control group (p = 0.0046), Ad5F35ET1-EGFP-shNC group (p = 0.0245), Ad5F35ET1-shrSulf group (p = 0.0426), and Ad5F35ET1-rbFGF group (p = 0.2853). The results demonstrated that this strategy may be a candidate therapy for chronic injury repair.","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10798112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The expression of BMP, integrin, ZEB2 in ovarian high-grade serous carcinoma in relation with lymph node metastasis. BMP、整合素、ZEB2在卵巢高级别浆液性癌中表达与淋巴结转移的关系。

IF 1.8 4区生物学

Growth factors Pub Date : 2022-08-01 Epub Date: 2022-07-22 DOI: 10.1080/08977194.2022.2099849

Elham Bahador Zırh, Elif Taşar Kapaklı, Anıl Dolgun, Alp Usubütün, Naciye Dilara Zeybek

{"title":"The expression of BMP, integrin, ZEB2 in ovarian high-grade serous carcinoma in relation with lymph node metastasis.","authors":"Elham Bahador Zırh, Elif Taşar Kapaklı, Anıl Dolgun, Alp Usubütün, Naciye Dilara Zeybek","doi":"10.1080/08977194.2022.2099849","DOIUrl":"https://doi.org/10.1080/08977194.2022.2099849","url":null,"abstract":"Ovarian cancer (OC) is clinically important because it is diagnosed late and has metastasis when it is diagnosed. Mortality risk increases 2.75 times in the presence of lymph node (LN) metastasis. During metastasis, many molecules including BMPs originated from stroma, and tumor cells participate through transcription factors and integrins for cytoskeleton regulation during cell migration. We hypothesized an inverse correlation between BMP2 and BMP7 along with changes in ZEB2, and integrin α5β1 in high-grade OCs in relation to LN metastasis. The BMP2 immunoreactivity was strong along with strong ZEB2 and weak integrins' immunoreactivity in samples with LN metastasis. Strong immunoreactivity of BMP7 was accompanied by strong immunoreactivity of integrins in the samples without LN metastasis. Study results showed BMP2's strong positive immunoreactivity and weak BMP7 immunoreactivity in tumor cells with a significantly weak inverse correlation. This inverse correlation should be considered as both BMPs have different effects in the window of cancer progression and invasion.","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40530970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The cellular and molecular mediators of metastasis to the lung. 肺转移的细胞和分子介质。

IF 1.8 4区生物学

Growth factors Pub Date : 2022-08-01 Epub Date: 2022-07-21 DOI: 10.1080/08977194.2022.2087520

Oliver Cucanic, Rae H Farnsworth, Steven A Stacker

引用次数: 4