Network pharmacology-and molecular docking-based investigation of Danggui blood-supplementing decoction in ischaemic stroke.

IF 1.8 4区生物学 Q4 CELL BIOLOGY

Growth factors Pub Date : 2024-02-01 Epub Date: 2024-01-31 DOI:10.1080/08977194.2023.2277755

Jinling Zhang, Ruiqing Li, Yang Yu, Weijia Sun, Chengshi Zhang, Haijun Wang

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引用次数: 0

Abstract

Danggui blood-supplementing decoction (DBsD) is an herbal preparation treating several diseases including stroke. The present study sought to investigate the potential mechanism of DBsD in ischaemic stroke (IS) using network pharmacology, molecular docking, and cell experiment. Based on the protein-protein (PPI) network analysis, MAPK1 (0.51, 12), KNG1 (0.57, 28), and TNF (0.64, 39) were found with relatively good performance in degree and closeness centrality. The functional enrichment analysis revealed that DBsD contributed to IS-related biological processes, molecule function, and presynaptic/postsynaptic cellular components. Pathway enrichment indicated that DBsD might protect IS by modulating multi-signalling pathways including the sphingolipid signalling pathway. Molecular docking verified the stigmasterol-KNG1, bifendate-TNF, and formononetin-MAPK1 pairs. Cell experiments confirmed the involvement of KNG1 and sphingolipid signalling pathway in hippocampal neuronal cell apoptosis. This study showed that DBsD can protect neuronal cell injury after IS through multiple components, multiple targets, and multiple pathways.

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当归补血汤治疗缺血性脑卒中的网络药理学和分子对接研究。

当归补血汤是治疗脑卒中等多种疾病的中药制剂。本研究试图通过网络药理学、分子对接和细胞实验来研究DBsD在缺血性中风（IS）中的潜在机制。基于蛋白质-蛋白质（PPI）网络分析，发现MAPK1（0.51，12）、KNG1（0.57，28）和TNF（0.64，39）在程度和接近中心性方面具有相对良好的表现。功能富集分析显示，DBsD有助于IS相关的生物过程、分子功能和突触前/突触后细胞成分。通路富集表明，DBsD可能通过调节包括鞘脂信号通路在内的多种信号通路来保护IS。分子对接验证了豆甾醇-KNG1、双酯TNF和甲素-MAPK1对。细胞实验证实了KNG1和鞘脂信号通路参与海马神经元细胞凋亡。本研究表明，DBsD可以通过多种成分、多种靶点和多种途径保护IS后的神经元细胞损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Growth factors 生物-内分泌学与代谢

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications