Genes to Cells最新文献_第2页

Staphylococcal γ-Hemolysin AB and γ-Hemolysin CB Differentially Activate Murine Bone Marrow-Derived Mast Cells 葡萄球菌γ-溶血素AB和γ-溶血素CB对小鼠骨髓源性肥大细胞的差异激活

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-09-03 DOI: 10.1111/gtc.70047

Hikaru Inoue, Haruka Sakakibara, Shion Kamada, Ayana Ogata, Kazuhito Hayashi, Shigeaki Hida, Saotomo Itoh

{"title":"Staphylococcal γ-Hemolysin AB and γ-Hemolysin CB Differentially Activate Murine Bone Marrow-Derived Mast Cells","authors":"Hikaru Inoue, Haruka Sakakibara, Shion Kamada, Ayana Ogata, Kazuhito Hayashi, Shigeaki Hida, Saotomo Itoh","doi":"10.1111/gtc.70047","DOIUrl":"https://doi.org/10.1111/gtc.70047","url":null,"abstract":"<div>\u0000 \u0000 Staphylococcus aureus (S. aureus) produces various bicomponent pore-forming toxins (PFTs), including the γ-hemolysins (HlgAB and HlgCB) and leukocidins (LukAB and LukED). This study aimed to examine the effect of PFTs on murine bone marrow-derived mast cells (BMMCs). All the PFTs assessed in this study (HlgAB, HlgCB, LukAB, and LukED) were found to bind to BMMCs. Specifically, HlgAB and LukED, but not HlgCB or LukAB, induced membrane damage. Furthermore, only HlgAB induced BMMC degranulation, whereas HlgAB and HlgCB significantly augmented the degranulation caused by ionophore, immunocomplex, and staphylococcal δ-toxin. The augmentation of degranulation by HlgAB was impaired when a pore-formation defect mutant of HlgB (HlgBΔstem) was used. Conversely, the augmentation by HlgCB was unaffected following the use of HlgBΔstem, suggesting that HlgAB but not HlgCB augments degranulation in a pore-formation-dependent manner. These results highlight the novel roles for the staphylococcal γ-hemolysins HlgAB and HlgCB, as they differentially affect the degranulation of mast cells in the effector phase of allergic inflammation.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 5","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144929715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of Nuclear Protein Dyro Causes Abnormalities in Nurse Cell Nuclei and Abort Oogenesis at Mid-Oogenesis Checkpoint 核蛋白Dyro缺失导致护士细胞核异常和卵子发生中期流产

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-09-01 DOI: 10.1111/gtc.70045

Takamoto Shima, Yuuki Kawabata, Yoshimasa Yagi

{"title":"Loss of Nuclear Protein Dyro Causes Abnormalities in Nurse Cell Nuclei and Abort Oogenesis at Mid-Oogenesis Checkpoint","authors":"Takamoto Shima, Yuuki Kawabata, Yoshimasa Yagi","doi":"10.1111/gtc.70045","DOIUrl":"https://doi.org/10.1111/gtc.70045","url":null,"abstract":"<div>\u0000 \u0000 The mid-oogenesis checkpoint in Drosophila melanogaster functions to optimize nutrient usage by triggering abortion of oogenesis when females are starved or when developmental defects arise in the egg chamber. In the Dyro mutant, which encodes a nuclear factor, oogenesis is aborted during stages 8–9, corresponding to the mid-oogenesis checkpoint. To investigate the relationship between Dyro and this checkpoint, we analyzed the phenotype of the Dyro mutant. Mosaic analysis showed that loss of Dyro in germline cells results in female sterility. Although inhibition of programmed cell death suppressed germline cell death during oogenesis, it failed to rescue the fertility of Dyro mutants, suggesting that oogenesis arrest in the Dyro mutant is not due to misregulation of the cell death signal. We then examined germline cell defects in the Dyro mutant and observed morphological abnormalities in the nucleoli and chromosomes of nurse cells. The chromosomes in Dyro mutant nurse cells were not fully dispersed, and the nucleoli were confined to small spaces between thickened chromosomes. These findings suggest that Dyro plays an important role in nurse cells and that loss of Dyro leads to defects in the chromosomes and nuclei of nurse cells, which leads to abortion of oogenesis.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 5","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144927516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maturation Status of Rat Lung Epithelial Cells in Interspecies Chimeras Generated by Blastocyst Complementation 胚泡互补产生的种间嵌合体中大鼠肺上皮细胞的成熟状态

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-08-21 DOI: 10.1111/gtc.70046

Yamato Murata, Shunsuke Yuri, Masahito Ikawa, Ayako Isotani

{"title":"Maturation Status of Rat Lung Epithelial Cells in Interspecies Chimeras Generated by Blastocyst Complementation","authors":"Yamato Murata, Shunsuke Yuri, Masahito Ikawa, Ayako Isotani","doi":"10.1111/gtc.70046","DOIUrl":"https://doi.org/10.1111/gtc.70046","url":null,"abstract":"<div>\u0000 \u0000 This study investigates the maturation status of rat lung epithelial cells in interspecies chimeras generated via blastocyst complementation (BC) using Fgfr2b-knockout mouse embryos. In our previous study, we succeeded in generating rat-derived lung epithelium in interspecies chimeras using tetraploid complementation; however, the resulting cells remained immature and failed to support respiratory function. In this study, we established two Fgfr2b-KO mouse lines via CRISPR/Cas9 and injected GFP-labeled rat embryonic stem (ES) cells into blastocysts, which were then transferred to pseudopregnant female mice. Comparative histological analysis of airway spaces between wild-type rat lungs (E19.5–E21.5) and BC chimeras revealed that BC lungs reached a maturation stage comparable to E20.5–E21.5 rat lungs. Quantitative Polymerase Chain Reaction of key epithelial maturation markers—including ENaC subunits, Aqp5, and surfactant protein genes—demonstrated late-gestational upregulation in wild-type rat lungs, while expression levels in BC lungs exhibited significant inter-individual variability, corresponding to stages between E19.5 and E21.5 in wild-type rats. These findings suggest that the mouse host environment may partially promote maturation of rat-derived lung epithelial cells; however, additional mechanisms are likely required to achieve functional respiratory capacity.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 5","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144885092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ERSAtool: A User-Friendly R/Shiny Comprehensive Transcriptomic Analysis Interface Suitable for Education ERSAtool：一个用户友好的R/Shiny综合转录组分析接口适合教育

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-08-18 DOI: 10.1111/gtc.70044

Sujith Taridalu, Ayyappa Kumar Sista Kameshwar, Masako Suzuki

{"title":"ERSAtool: A User-Friendly R/Shiny Comprehensive Transcriptomic Analysis Interface Suitable for Education","authors":"Sujith Taridalu, Ayyappa Kumar Sista Kameshwar, Masako Suzuki","doi":"10.1111/gtc.70044","DOIUrl":"https://doi.org/10.1111/gtc.70044","url":null,"abstract":"RNA sequencing (RNA-seq) has become an essential technology for assessing gene expression profiles in biomedical research. However, the coding complexity of RNA-seq data analysis remains a significant barrier for students and researchers without extensive bioinformatics expertise. We present the Educational RNA-Seq Analysis tool (ERSAtool), a comprehensive R/Shiny interface that provides an intuitive graphical visualization of the complete RNA-seq analysis workflow. The application is built on established Bioconductor packages and upholds high standards in analyses while significantly reducing the technical expertise required to conduct sophisticated transcriptomic analyses. ERSAtool supports various input formats, such as raw count matrices and STAR alignment outputs. It generates sample information metadata through direct integration with the international public repository, Gene Expression Omnibus (GEO). The application guides users through normalization, data visualization, differential expression analysis, and functional interpretation using Gene Ontology and Gene Set Enrichment Analyses. All results can be compiled into comprehensive, downloadable reports that enhance reproducibility and knowledge sharing. The design targets features that support educational use, making it especially helpful for teaching transcriptomics in undergraduate to graduate-level bioinformatics courses and enhancing access to advanced transcriptomic analysis, potentially accelerating discoveries across various biological fields. ERSAtool is available for free at https://github.com/SuzukiLabTAMU/ERSAtool.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 5","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.70044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Meeting Report on the Fifth International Conference of the Chromosomal SMC Complexes 第五届染色体SMC复合体国际会议报告

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-08-05 DOI: 10.1111/gtc.70039

Hironori Niki, Yasuto Murayama

引用次数: 0

Functional Differences Between SIRPα Splice Isoforms SIRPα剪接异构体的功能差异

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-08-05 DOI: 10.1111/gtc.70041

Mihoko Kajita, Yojiro Matsui, Kotaro Sugimoto, Shuto Takeuchi, Shota Matsumoto, Takahiro Okumura, Hiroyuki Kajiura, Kazuki Motomura, Atsushi Takeda, Tomomi Koshiyama, Kyoko Shirakabe

{"title":"Functional Differences Between SIRPα Splice Isoforms","authors":"Mihoko Kajita, Yojiro Matsui, Kotaro Sugimoto, Shuto Takeuchi, Shota Matsumoto, Takahiro Okumura, Hiroyuki Kajiura, Kazuki Motomura, Atsushi Takeda, Tomomi Koshiyama, Kyoko Shirakabe","doi":"10.1111/gtc.70041","DOIUrl":"https://doi.org/10.1111/gtc.70041","url":null,"abstract":"Signal regulatory protein (SIRP) α, an inhibitory receptor belonging to the immunoglobulin (Ig) superfamily is abundantly expressed in phagocytes such as macrophages. CD47, the ligand for SIRPα, is expressed in most healthy cells, and called “don't eat me” signal because it binds to SIRPα on the surface of macrophages and inhibits phagocytosis. SIRPα has multiple splice isoforms, but most functional analyses have been carried out using long SIRPα, the SIRPα isoform with three extracellular Ig domains. In this study, we analyzed the expression and function of short SIRPα, an SIRPα isoform with only one extracellular Ig domain. In resting mouse macrophage Raw 264.7 cells, the short and long SIRPα mRNA expression levels were similar, and the proportion of short SIRPα mRNA decreased substantially after endotoxin stimulation. Short SIRPα bound to CD47 as same as long SIRPα, however, did not suppress the phagocytosis of recombinant CD47-coated beads, unlike long SIRPα. These results suggest that short SIRPα may be a “don't eat me” signal regulator with different expression and function from long SIRPα.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 5","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.70041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144782757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paralog-Dependent Specialization of Paf1C Subunit, Ctr9, for Sex Chromosome Gene Regulation and Male Germline Differentiation in Drosophila 果蝇性染色体基因调控和雄性生殖系分化中Paf1C亚基Ctr9的同源依赖特化

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-08-05 DOI: 10.1111/gtc.70040

Toshie Kai, Jinglan Zheng, Taichiro Iki

{"title":"Paralog-Dependent Specialization of Paf1C Subunit, Ctr9, for Sex Chromosome Gene Regulation and Male Germline Differentiation in Drosophila","authors":"Toshie Kai, Jinglan Zheng, Taichiro Iki","doi":"10.1111/gtc.70040","DOIUrl":"https://doi.org/10.1111/gtc.70040","url":null,"abstract":"Testis-specific gene regulatory mechanisms govern the differentiation of germ cells into mature sperm. However, the molecular underpinnings are not fully elucidated. Here, we show the subunits forming Paf1C, a transcription regulator complex conserved across eukaryotes, have their individual paralogs predominantly expressed in Drosophila testes. One of these, namely, Ctr9 paralog enriched in testes (Ctr9t) was found to play a critical and nonredundant role in postmeiotic spermatid differentiation and male fertility in D. melanogaster. A proximity proteome analysis provides evidence that Ctr9t prefers the interaction between paralog members. We show endogenous Ctr9t is expressed and functional in germ cells at spermatocyte stages, accumulating in a distinct compartment within the nucleolus. There, Ctr9t co-localizes with Spermatocyte arrest (Sa), a testis-specific paralog of TATA-binding protein (TBP)-associated factor 8 (TAF8). We further demonstrate that ctr9t function is crucial for maintaining Sa in the nucleolus, but not vice versa. Transcriptome profiling reveals that Ctr9t acts as an activator for the set of male fertility genes on the Y chromosome, but it also acts as a global repressor of X chromosome genes. Collectively, our results shed light on the nucleolus-associated, paralog-dependent regulation of gene expression from sex chromosomes, which ensures the terminal differentiation of male germ cells.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 5","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144782584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TOB Proteins Repress Translation via the CCR4–NOT Deadenylase Complex Independent of Deadenylation TOB蛋白通过独立于去烯化的CCR4-NOT去烯化酶复合物抑制翻译

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-08-04 DOI: 10.1111/gtc.70042

Kanae Miyazaki, Takumi Tomohiro, Yoshinori Funakami, Akira Fukao, Toru Suzuki, Tadashi Yamamoto, Toshinobu Fujiwara

{"title":"TOB Proteins Repress Translation via the CCR4–NOT Deadenylase Complex Independent of Deadenylation","authors":"Kanae Miyazaki, Takumi Tomohiro, Yoshinori Funakami, Akira Fukao, Toru Suzuki, Tadashi Yamamoto, Toshinobu Fujiwara","doi":"10.1111/gtc.70042","DOIUrl":"https://doi.org/10.1111/gtc.70042","url":null,"abstract":"<div>\u0000 \u0000 Transducer of ErbB2 (TOB) proteins have been shown to promote mRNA decay through interactions with the CCR4–NOT complex and poly(A)-binding protein (PABP). While their role in deadenylation-mediated mRNA degradation is well established, their potential function in translational control remains to be elucidated. Here, we employed an in vitro translation system combined with an RNA tethering strategy to examine the function of TOB1 and TOB2 in translation. Our results demonstrate that TOB1 and TOB2 act as repressors of translation initiation, independent of deadenylation. Notably, this translational repression selectively targets eIF4A-dependent translation, while translation driven by eIF4A-independent IRES elements remains unaffected. While the interaction between TOB proteins and PABP appears to be dispensable, as disruption of this interaction only partially reduces translational repression, the knockdown of CNOT1, the scaffold of the CCR4–NOT complex, substantially relieves this repression, highlighting its indispensable role in the mechanism. Collectively, our findings uncover a previously unrecognized function of TOB proteins as direct repressors of translation initiation, independent of mRNA decay, and highlight a specific reliance on eIF4A activity and CCR4–NOT complex integrity.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 5","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meeting Report on the 1st Asia & Pacific Bioinformatics Joint Conference (APBJC 2024) 第一届亚太生物信息学联席会议（APBJC 2024）会议报告

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-07-03 DOI: 10.1111/gtc.70038

Kiyoko F. Aoki-Kinoshita, Susumu Goto

引用次数: 0

Effect of Retinal on Dictyostelium Cells During Development 视网膜对盘状骨细胞发育的影响

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-07-02 DOI: 10.1111/gtc.70037

Kazuki Akiyama, Shuhei Tsuchihashi, Yusuke V. Morimoto

引用次数: 0