Loss of Nuclear Protein Dyro Causes Abnormalities in Nurse Cell Nuclei and Abort Oogenesis at Mid-Oogenesis Checkpoint

IF 1.3 4区 生物学 Q4 CELL BIOLOGY
Genes to Cells Pub Date : 2025-09-01 DOI:10.1111/gtc.70045
Takamoto Shima, Yuuki Kawabata, Yoshimasa Yagi
{"title":"Loss of Nuclear Protein Dyro Causes Abnormalities in Nurse Cell Nuclei and Abort Oogenesis at Mid-Oogenesis Checkpoint","authors":"Takamoto Shima,&nbsp;Yuuki Kawabata,&nbsp;Yoshimasa Yagi","doi":"10.1111/gtc.70045","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>The mid-oogenesis checkpoint in <i>Drosophila melanogaster</i> functions to optimize nutrient usage by triggering abortion of oogenesis when females are starved or when developmental defects arise in the egg chamber. In the <i>Dyro</i> mutant, which encodes a nuclear factor, oogenesis is aborted during stages 8–9, corresponding to the mid-oogenesis checkpoint. To investigate the relationship between <i>Dyro</i> and this checkpoint, we analyzed the phenotype of the <i>Dyro</i> mutant. Mosaic analysis showed that loss of Dyro in germline cells results in female sterility. Although inhibition of programmed cell death suppressed germline cell death during oogenesis, it failed to rescue the fertility of <i>Dyro</i> mutants, suggesting that oogenesis arrest in the <i>Dyro</i> mutant is not due to misregulation of the cell death signal. We then examined germline cell defects in the <i>Dyro</i> mutant and observed morphological abnormalities in the nucleoli and chromosomes of nurse cells. The chromosomes in <i>Dyro</i> mutant nurse cells were not fully dispersed, and the nucleoli were confined to small spaces between thickened chromosomes. These findings suggest that <i>Dyro</i> plays an important role in nurse cells and that loss of Dyro leads to defects in the chromosomes and nuclei of nurse cells, which leads to abortion of oogenesis.</p>\n </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 5","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genes to Cells","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/gtc.70045","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The mid-oogenesis checkpoint in Drosophila melanogaster functions to optimize nutrient usage by triggering abortion of oogenesis when females are starved or when developmental defects arise in the egg chamber. In the Dyro mutant, which encodes a nuclear factor, oogenesis is aborted during stages 8–9, corresponding to the mid-oogenesis checkpoint. To investigate the relationship between Dyro and this checkpoint, we analyzed the phenotype of the Dyro mutant. Mosaic analysis showed that loss of Dyro in germline cells results in female sterility. Although inhibition of programmed cell death suppressed germline cell death during oogenesis, it failed to rescue the fertility of Dyro mutants, suggesting that oogenesis arrest in the Dyro mutant is not due to misregulation of the cell death signal. We then examined germline cell defects in the Dyro mutant and observed morphological abnormalities in the nucleoli and chromosomes of nurse cells. The chromosomes in Dyro mutant nurse cells were not fully dispersed, and the nucleoli were confined to small spaces between thickened chromosomes. These findings suggest that Dyro plays an important role in nurse cells and that loss of Dyro leads to defects in the chromosomes and nuclei of nurse cells, which leads to abortion of oogenesis.

Abstract Image

核蛋白Dyro缺失导致护士细胞核异常和卵子发生中期流产
当雌性饥饿或卵室出现发育缺陷时,黑腹果蝇的中期卵发生检查点通过触发卵发生流产来优化营养利用。在编码核因子的Dyro突变体中,卵子发生在8-9阶段终止,对应于卵子发生中期检查点。为了研究Dyro和这个检查点之间的关系,我们分析了Dyro突变体的表型。嵌合分析表明,生殖系细胞中Dyro的缺失导致雌性不育。尽管抑制程序性细胞死亡抑制了卵子发生过程中的种系细胞死亡,但它未能挽救Dyro突变体的生育能力,这表明Dyro突变体的卵子发生停滞不是由于细胞死亡信号的错误调节。然后,我们检查了Dyro突变体的种系细胞缺陷,并观察到核仁和染色体的形态学异常。Dyro突变型护士细胞的染色体不完全分散,核仁被限制在增厚的染色体之间的小空间内。这些发现表明Dyro在哺乳细胞中起着重要的作用,Dyro的缺失会导致哺乳细胞染色体和细胞核的缺陷,从而导致卵子发生流产。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Genes to Cells
Genes to Cells 生物-细胞生物学
CiteScore
3.40
自引率
0.00%
发文量
71
审稿时长
3 months
期刊介绍: Genes to Cells provides an international forum for the publication of papers describing important aspects of molecular and cellular biology. The journal aims to present papers that provide conceptual advance in the relevant field. Particular emphasis will be placed on work aimed at understanding the basic mechanisms underlying biological events.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信