{"title":"Loss of Nuclear Protein Dyro Causes Abnormalities in Nurse Cell Nuclei and Abort Oogenesis at Mid-Oogenesis Checkpoint","authors":"Takamoto Shima, Yuuki Kawabata, Yoshimasa Yagi","doi":"10.1111/gtc.70045","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>The mid-oogenesis checkpoint in <i>Drosophila melanogaster</i> functions to optimize nutrient usage by triggering abortion of oogenesis when females are starved or when developmental defects arise in the egg chamber. In the <i>Dyro</i> mutant, which encodes a nuclear factor, oogenesis is aborted during stages 8–9, corresponding to the mid-oogenesis checkpoint. To investigate the relationship between <i>Dyro</i> and this checkpoint, we analyzed the phenotype of the <i>Dyro</i> mutant. Mosaic analysis showed that loss of Dyro in germline cells results in female sterility. Although inhibition of programmed cell death suppressed germline cell death during oogenesis, it failed to rescue the fertility of <i>Dyro</i> mutants, suggesting that oogenesis arrest in the <i>Dyro</i> mutant is not due to misregulation of the cell death signal. We then examined germline cell defects in the <i>Dyro</i> mutant and observed morphological abnormalities in the nucleoli and chromosomes of nurse cells. The chromosomes in <i>Dyro</i> mutant nurse cells were not fully dispersed, and the nucleoli were confined to small spaces between thickened chromosomes. These findings suggest that <i>Dyro</i> plays an important role in nurse cells and that loss of Dyro leads to defects in the chromosomes and nuclei of nurse cells, which leads to abortion of oogenesis.</p>\n </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 5","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genes to Cells","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/gtc.70045","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The mid-oogenesis checkpoint in Drosophila melanogaster functions to optimize nutrient usage by triggering abortion of oogenesis when females are starved or when developmental defects arise in the egg chamber. In the Dyro mutant, which encodes a nuclear factor, oogenesis is aborted during stages 8–9, corresponding to the mid-oogenesis checkpoint. To investigate the relationship between Dyro and this checkpoint, we analyzed the phenotype of the Dyro mutant. Mosaic analysis showed that loss of Dyro in germline cells results in female sterility. Although inhibition of programmed cell death suppressed germline cell death during oogenesis, it failed to rescue the fertility of Dyro mutants, suggesting that oogenesis arrest in the Dyro mutant is not due to misregulation of the cell death signal. We then examined germline cell defects in the Dyro mutant and observed morphological abnormalities in the nucleoli and chromosomes of nurse cells. The chromosomes in Dyro mutant nurse cells were not fully dispersed, and the nucleoli were confined to small spaces between thickened chromosomes. These findings suggest that Dyro plays an important role in nurse cells and that loss of Dyro leads to defects in the chromosomes and nuclei of nurse cells, which leads to abortion of oogenesis.
期刊介绍:
Genes to Cells provides an international forum for the publication of papers describing important aspects of molecular and cellular biology. The journal aims to present papers that provide conceptual advance in the relevant field. Particular emphasis will be placed on work aimed at understanding the basic mechanisms underlying biological events.