{"title":"葡萄球菌γ-溶血素AB和γ-溶血素CB对小鼠骨髓源性肥大细胞的差异激活","authors":"Hikaru Inoue, Haruka Sakakibara, Shion Kamada, Ayana Ogata, Kazuhito Hayashi, Shigeaki Hida, Saotomo Itoh","doi":"10.1111/gtc.70047","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p><i>Staphylococcus aureus</i> (<i>S. aureus</i>) produces various bicomponent pore-forming toxins (PFTs), including the γ-hemolysins (HlgAB and HlgCB) and leukocidins (LukAB and LukED). This study aimed to examine the effect of PFTs on murine bone marrow-derived mast cells (BMMCs). All the PFTs assessed in this study (HlgAB, HlgCB, LukAB, and LukED) were found to bind to BMMCs. Specifically, HlgAB and LukED, but not HlgCB or LukAB, induced membrane damage. Furthermore, only HlgAB induced BMMC degranulation, whereas HlgAB and HlgCB significantly augmented the degranulation caused by ionophore, immunocomplex, and staphylococcal δ-toxin. The augmentation of degranulation by HlgAB was impaired when a pore-formation defect mutant of HlgB (HlgBΔstem) was used. Conversely, the augmentation by HlgCB was unaffected following the use of HlgBΔstem, suggesting that HlgAB but not HlgCB augments degranulation in a pore-formation-dependent manner. These results highlight the novel roles for the staphylococcal γ-hemolysins HlgAB and HlgCB, as they differentially affect the degranulation of mast cells in the effector phase of allergic inflammation.</p>\n </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 5","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Staphylococcal γ-Hemolysin AB and γ-Hemolysin CB Differentially Activate Murine Bone Marrow-Derived Mast Cells\",\"authors\":\"Hikaru Inoue, Haruka Sakakibara, Shion Kamada, Ayana Ogata, Kazuhito Hayashi, Shigeaki Hida, Saotomo Itoh\",\"doi\":\"10.1111/gtc.70047\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p><i>Staphylococcus aureus</i> (<i>S. aureus</i>) produces various bicomponent pore-forming toxins (PFTs), including the γ-hemolysins (HlgAB and HlgCB) and leukocidins (LukAB and LukED). This study aimed to examine the effect of PFTs on murine bone marrow-derived mast cells (BMMCs). All the PFTs assessed in this study (HlgAB, HlgCB, LukAB, and LukED) were found to bind to BMMCs. Specifically, HlgAB and LukED, but not HlgCB or LukAB, induced membrane damage. Furthermore, only HlgAB induced BMMC degranulation, whereas HlgAB and HlgCB significantly augmented the degranulation caused by ionophore, immunocomplex, and staphylococcal δ-toxin. The augmentation of degranulation by HlgAB was impaired when a pore-formation defect mutant of HlgB (HlgBΔstem) was used. Conversely, the augmentation by HlgCB was unaffected following the use of HlgBΔstem, suggesting that HlgAB but not HlgCB augments degranulation in a pore-formation-dependent manner. These results highlight the novel roles for the staphylococcal γ-hemolysins HlgAB and HlgCB, as they differentially affect the degranulation of mast cells in the effector phase of allergic inflammation.</p>\\n </div>\",\"PeriodicalId\":12742,\"journal\":{\"name\":\"Genes to Cells\",\"volume\":\"30 5\",\"pages\":\"\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-09-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genes to Cells\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/gtc.70047\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genes to Cells","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/gtc.70047","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Staphylococcal γ-Hemolysin AB and γ-Hemolysin CB Differentially Activate Murine Bone Marrow-Derived Mast Cells
Staphylococcus aureus (S. aureus) produces various bicomponent pore-forming toxins (PFTs), including the γ-hemolysins (HlgAB and HlgCB) and leukocidins (LukAB and LukED). This study aimed to examine the effect of PFTs on murine bone marrow-derived mast cells (BMMCs). All the PFTs assessed in this study (HlgAB, HlgCB, LukAB, and LukED) were found to bind to BMMCs. Specifically, HlgAB and LukED, but not HlgCB or LukAB, induced membrane damage. Furthermore, only HlgAB induced BMMC degranulation, whereas HlgAB and HlgCB significantly augmented the degranulation caused by ionophore, immunocomplex, and staphylococcal δ-toxin. The augmentation of degranulation by HlgAB was impaired when a pore-formation defect mutant of HlgB (HlgBΔstem) was used. Conversely, the augmentation by HlgCB was unaffected following the use of HlgBΔstem, suggesting that HlgAB but not HlgCB augments degranulation in a pore-formation-dependent manner. These results highlight the novel roles for the staphylococcal γ-hemolysins HlgAB and HlgCB, as they differentially affect the degranulation of mast cells in the effector phase of allergic inflammation.
期刊介绍:
Genes to Cells provides an international forum for the publication of papers describing important aspects of molecular and cellular biology. The journal aims to present papers that provide conceptual advance in the relevant field. Particular emphasis will be placed on work aimed at understanding the basic mechanisms underlying biological events.