Staphylococcal γ-Hemolysin AB and γ-Hemolysin CB Differentially Activate Murine Bone Marrow-Derived Mast Cells

IF 1.3 4区 生物学 Q4 CELL BIOLOGY
Genes to Cells Pub Date : 2025-09-03 DOI:10.1111/gtc.70047
Hikaru Inoue, Haruka Sakakibara, Shion Kamada, Ayana Ogata, Kazuhito Hayashi, Shigeaki Hida, Saotomo Itoh
{"title":"Staphylococcal γ-Hemolysin AB and γ-Hemolysin CB Differentially Activate Murine Bone Marrow-Derived Mast Cells","authors":"Hikaru Inoue,&nbsp;Haruka Sakakibara,&nbsp;Shion Kamada,&nbsp;Ayana Ogata,&nbsp;Kazuhito Hayashi,&nbsp;Shigeaki Hida,&nbsp;Saotomo Itoh","doi":"10.1111/gtc.70047","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p><i>Staphylococcus aureus</i> (<i>S. aureus</i>) produces various bicomponent pore-forming toxins (PFTs), including the γ-hemolysins (HlgAB and HlgCB) and leukocidins (LukAB and LukED). This study aimed to examine the effect of PFTs on murine bone marrow-derived mast cells (BMMCs). All the PFTs assessed in this study (HlgAB, HlgCB, LukAB, and LukED) were found to bind to BMMCs. Specifically, HlgAB and LukED, but not HlgCB or LukAB, induced membrane damage. Furthermore, only HlgAB induced BMMC degranulation, whereas HlgAB and HlgCB significantly augmented the degranulation caused by ionophore, immunocomplex, and staphylococcal δ-toxin. The augmentation of degranulation by HlgAB was impaired when a pore-formation defect mutant of HlgB (HlgBΔstem) was used. Conversely, the augmentation by HlgCB was unaffected following the use of HlgBΔstem, suggesting that HlgAB but not HlgCB augments degranulation in a pore-formation-dependent manner. These results highlight the novel roles for the staphylococcal γ-hemolysins HlgAB and HlgCB, as they differentially affect the degranulation of mast cells in the effector phase of allergic inflammation.</p>\n </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 5","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genes to Cells","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/gtc.70047","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Staphylococcus aureus (S. aureus) produces various bicomponent pore-forming toxins (PFTs), including the γ-hemolysins (HlgAB and HlgCB) and leukocidins (LukAB and LukED). This study aimed to examine the effect of PFTs on murine bone marrow-derived mast cells (BMMCs). All the PFTs assessed in this study (HlgAB, HlgCB, LukAB, and LukED) were found to bind to BMMCs. Specifically, HlgAB and LukED, but not HlgCB or LukAB, induced membrane damage. Furthermore, only HlgAB induced BMMC degranulation, whereas HlgAB and HlgCB significantly augmented the degranulation caused by ionophore, immunocomplex, and staphylococcal δ-toxin. The augmentation of degranulation by HlgAB was impaired when a pore-formation defect mutant of HlgB (HlgBΔstem) was used. Conversely, the augmentation by HlgCB was unaffected following the use of HlgBΔstem, suggesting that HlgAB but not HlgCB augments degranulation in a pore-formation-dependent manner. These results highlight the novel roles for the staphylococcal γ-hemolysins HlgAB and HlgCB, as they differentially affect the degranulation of mast cells in the effector phase of allergic inflammation.

Abstract Image

葡萄球菌γ-溶血素AB和γ-溶血素CB对小鼠骨髓源性肥大细胞的差异激活
金黄色葡萄球菌(S. aureus)产生多种双组分成孔毒素(pft),包括γ-溶血素(HlgAB和HlgCB)和杀白细胞素(LukAB和luk)。本研究旨在探讨PFTs对小鼠骨髓源性肥大细胞(BMMCs)的影响。本研究评估的所有PFTs (HlgAB、HlgCB、LukAB和luk)均与BMMCs结合。具体来说,HlgAB和LukED诱导膜损伤,而不是HlgAB和LukAB。此外,只有HlgAB能诱导BMMC脱粒,而HlgAB和HlgCB能显著增强离子载体、免疫复合物和葡萄球菌δ-毒素引起的BMMC脱粒。当使用HlgB的孔隙形成缺陷突变体(HlgBΔstem)时,HlgAB对脱颗粒的增强作用受到损害。相反,在使用HlgBΔstem后,HlgCB的增强作用不受影响,这表明HlgAB而不是HlgCB以依赖于孔隙形成的方式增强脱粒。这些结果强调了葡萄球菌γ-溶血素HlgAB和HlgCB的新作用,因为它们在过敏性炎症的效应期对肥大细胞的脱颗粒有不同的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Genes to Cells
Genes to Cells 生物-细胞生物学
CiteScore
3.40
自引率
0.00%
发文量
71
审稿时长
3 months
期刊介绍: Genes to Cells provides an international forum for the publication of papers describing important aspects of molecular and cellular biology. The journal aims to present papers that provide conceptual advance in the relevant field. Particular emphasis will be placed on work aimed at understanding the basic mechanisms underlying biological events.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信