Genes to Cells最新文献_第10页

Yeast Tor complex 1 phosphorylates eIF4E-binding protein, Caf20 酵母Tor复合物1磷酸化eif4e结合蛋白Caf20。

IF 2.1 4区生物学

Genes to Cells Pub Date : 2023-09-12 DOI: 10.1111/gtc.13067

Yoshiaki Kamada, Ryoko Ando, Shingo Izawa, Akira Matsuura

引用次数: 1

Lumicrine signaling: Extracellular regulation of sperm maturation in the male reproductive tract lumen 尿嘧啶信号传导:男性生殖道腔内精子成熟的细胞外调控。

IF 2.1 4区生物学

Genes to Cells Pub Date : 2023-09-11 DOI: 10.1111/gtc.13066

Daiji Kiyozumi

{"title":"Lumicrine signaling: Extracellular regulation of sperm maturation in the male reproductive tract lumen","authors":"Daiji Kiyozumi","doi":"10.1111/gtc.13066","DOIUrl":"10.1111/gtc.13066","url":null,"abstract":"The behaviors of cells, tissues, and organs are controlled by the extracellular environment in addition to their autonomous regulatory system. Dysfunction of extracellular regulatory mechanisms affects not only the development and survival of organisms but also successful reproduction. In this review article, a novel extracellular regulatory mechanism regulating the mammalian male reproductive ability will be briefly summarized. In terrestrial vertebrates, spermatozoa generated in the testis are transported through the lumen of the male reproductive tract and become functionally mature during the transport. Recent studies with gene-modified animals are unveiling the luminal extracellular environment of the reproductive tract to function not only as the pathway of sperm transport and the site of sperm maturation but also as the channel for cellular communication to regulate sperm maturation. Of special interest is the molecular mechanism of lumicrine signaling, a transluminal secreted signal transduction in the male reproductive tract lumen as a master regulator of sperm maturation and male reproductive ability. The general significance of such transluminal signaling in the context of cell biology will also be discussed.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.13066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10213386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wnt10b knockdown promotes UCP1 expression in brown adipose tissue in mice Wnt10b敲低可促进小鼠棕色脂肪组织中UCP1的表达。

IF 2.1 4区生物学

Genes to Cells Pub Date : 2023-09-10 DOI: 10.1111/gtc.13064

Yanxin Jia, Yan Liu, Shuang Liu, Yaxin Chang, Longfei Xu, Haifang Li

{"title":"Wnt10b knockdown promotes UCP1 expression in brown adipose tissue in mice","authors":"Yanxin Jia, Yan Liu, Shuang Liu, Yaxin Chang, Longfei Xu, Haifang Li","doi":"10.1111/gtc.13064","DOIUrl":"10.1111/gtc.13064","url":null,"abstract":"The effect of Wnt10b overexpression on adipose tissue development has been reported. However, the impact of Wnt10b knockdown on the function of brown adipose tissue (BAT) is yet largely unknown. Here, we used the CRISPR/Cas9 technique to generate Wnt10b-knockdown (Wnt10b+/−) mice. We compared the development and thermogenic gene expression of interscapular BAT (iBAT) between Wnt10b+/− and Wnt10b+/+ mice under a chow diet, high-fat diet (HFD), and cold exposure conditions. Moreover, the effect of Wnt10b knockdown on brown adipocyte function was tested via in vitro experiments. Results indicated that Wnt10b knockdown decreased the iBAT mass and the brown adipocyte size and enhanced thermogenic gene expression, including UCP1, under chow diet conditions. In addition, Wnt10b+/− mice appeared to be able to maintain their body temperature better than the control in a cold environment, accompanied by higher UCP1 protein expression. Intriguingly, even under HFD conditions, Wnt10b+/− mice still showed higher UCP1 expression, which was associated with an alleviated obesity phenotype. In vitro studies further evidenced the Wnt10b knockdown stimulation of UCP1 expression and suppression of the adipogenic program. This study indicates that Wnt10b knockdown enhances UCP1 expression and inhibits the adipogenic differentiation of brown adipocytes, providing a novel option for therapeutic interventions in adiposity.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10257126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential dependence on microbiota of IL-23/IL-22-dependent gene expression between the small- and large-intestinal epithelia 小肠上皮和大肠上皮之间IL-23/ il -22依赖基因表达对微生物群的差异依赖性

IF 2.1 4区生物学

Genes to Cells Pub Date : 2023-09-07 DOI: 10.1111/gtc.13065

Ayaka Nakatani, Ryu Okumura, Airi Ishibashi, Shota Okamoto, Kei Sakaki, Yuki Ito, Daisuke Okuzaki, Hidenori Inohara, Kiyoshi Takeda

{"title":"Differential dependence on microbiota of IL-23/IL-22-dependent gene expression between the small- and large-intestinal epithelia","authors":"Ayaka Nakatani, Ryu Okumura, Airi Ishibashi, Shota Okamoto, Kei Sakaki, Yuki Ito, Daisuke Okuzaki, Hidenori Inohara, Kiyoshi Takeda","doi":"10.1111/gtc.13065","DOIUrl":"10.1111/gtc.13065","url":null,"abstract":"In the intestine, interleukin (IL)-23 and IL-22 from immune cells in the lamina propria contribute to maintenance of the gut epithelial barrier through the induction of antimicrobial production and the promotion of epithelial cell proliferation. Several previous studies suggested that some of the functions of the IL-23/IL-22 axis on intestinal epithelial cells are shared between the small and large intestines. However, the similarities and differences of the IL-23/IL-22 axis on epithelial cells between these two anatomical sites remain unclear. Here, we comprehensively analyzed the gene expression of intestinal epithelial cells in the ileum and colon of germ-free, Il23−/−, and Il22−/− mice by RNA-sequencing. We found that while the IL-23/IL-22 axis is largely dependent on gut microbiota in the small intestine, it is much less dependent on it in the large intestine. In addition, the negative regulation of lipid metabolism in the epithelial cells by IL-23 and IL-22 in the small intestine was revealed, whereas the positive regulation of epithelial cell proliferation by IL-23 and IL-22 in the large intestine was highlighted. These findings shed light on the intestinal site-specific role of the IL-23/IL-22 axis in maintaining the physiological functions of intestinal epithelial cells.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10238833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validity of the cell-extracted proteome as a substrate pool for exploring phosphorylation motifs of kinases 细胞提取蛋白质组作为底物库探索激酶磷酸化基序的有效性。

IF 2.1 4区生物学

Genes to Cells Pub Date : 2023-09-02 DOI: 10.1111/gtc.13063

Tomoya Niinae, Naoyuki Sugiyama, Yasushi Ishihama

{"title":"Validity of the cell-extracted proteome as a substrate pool for exploring phosphorylation motifs of kinases","authors":"Tomoya Niinae, Naoyuki Sugiyama, Yasushi Ishihama","doi":"10.1111/gtc.13063","DOIUrl":"10.1111/gtc.13063","url":null,"abstract":"Three representative protein kinases with different substrate preferences, ERK1 (Pro-directed), CK2 (acidophilic), and PKA (basophilic), were used to investigate phosphorylation sequence motifs in substrate pools consisting of the proteomes from three different cell lines, MCF7 (human mammary carcinoma), HeLa (human cervical carcinoma), and Jurkat (human acute T-cell leukemia). Specifically, recombinant kinases were added to the cell-extracted proteomes to phosphorylate the substrates in vitro. After trypsin digestion, the phosphopeptides were enriched and subjected to nanoLC/MS/MS analysis to identify their phosphorylation sites on a large scale. By analyzing the obtained phosphorylation sites and their surrounding sequences, phosphorylation motifs were extracted for each kinase-substrate proteome pair. We found that each kinase exhibited the same set of phosphorylation motifs, independently of the substrate pool proteome. Furthermore, the identified motifs were also consistent with those found using a completely randomized peptide library. These results indicate that cell-extracted proteomes can provide kinase phosphorylation motifs with sufficient accuracy, even though their sequences are not completely random, supporting the robustness of phosphorylation motif identification based on phosphoproteome analysis of cell extracts as a substrate pool for a kinase of interest.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.13063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10137849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Daidara: A gigantic Gypsy LTR retrotransposon lineage in the springtail Allacma fusca genome 戴达拉：一个巨大的吉普赛人春尾Allacma fusca基因组中的LTR逆转录转座子谱系。

IF 2.1 4区生物学

Genes to Cells Pub Date : 2023-08-31 DOI: 10.1111/gtc.13062

Kenji K. Kojima

引用次数: 0

DHX36 maintains genomic integrity by unwinding G-quadruplexes DHX36通过解开G-四链体来保持基因组的完整性。

IF 2.1 4区生物学

Genes to Cells Pub Date : 2023-08-26 DOI: 10.1111/gtc.13061

Ayaka Mizumoto, Yuta Yokoyama, Tomoichiro Miyoshi, Masahiro Takikawa, Fuyuki Ishikawa, Mahito Sadaie

{"title":"DHX36 maintains genomic integrity by unwinding G-quadruplexes","authors":"Ayaka Mizumoto, Yuta Yokoyama, Tomoichiro Miyoshi, Masahiro Takikawa, Fuyuki Ishikawa, Mahito Sadaie","doi":"10.1111/gtc.13061","DOIUrl":"10.1111/gtc.13061","url":null,"abstract":"The guanine-rich stretch of single-stranded DNA (ssDNA) forms a G-quadruplex (G4) in a fraction of genic and intergenic chromosomal regions. The probability of G4 formation increases during events causing ssDNA generation, such as transcription and replication. In turn, G4 abrogates these events, leading to DNA damage. DHX36 unwinds G4-DNA in vitro and in human cells. However, its spatial correlation with G4-DNA in vivo and its role in genome maintenance remain unclear. Here, we demonstrate a connection between DHX36 and G4-DNA and its implications for genomic integrity. The nuclear localization of DHX36 overlapped with that of G4-DNA, RNA polymerase II, and a splicing-related factor. Depletion of DHX36 resulted in accumulated DNA damage, slower cell growth, and enhanced cell growth inhibition upon treatment with a G4-stabilizing compound; DHX36 expression reversed these defects. In contrast, the reversal upon expression of DHX36 mutants that could not bind G4 was imperfect. Thus, DHX36 may suppress DNA damage by promoting the clearance of G4-DNA for cell growth and survival. Our findings deepen the understanding of G4 resolution in the maintenance of genomic integrity.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.13061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10075468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ectopic expression of matrix metalloproteinases and filopodia extension via JNK activation are involved in the invasion of blood tumor cells in Drosophila mxc mutant 基质金属蛋白酶的异位表达和通过JNK激活的丝足细胞延伸参与了果蝇mxc突变体中血液肿瘤细胞的侵袭。

IF 2.1 4区生物学

Genes to Cells Pub Date : 2023-08-24 DOI: 10.1111/gtc.13060

Kazuki Takarada, Juri Kinoshita, Yoshihiro H. Inoue

{"title":"Ectopic expression of matrix metalloproteinases and filopodia extension via JNK activation are involved in the invasion of blood tumor cells in Drosophila mxc mutant","authors":"Kazuki Takarada, Juri Kinoshita, Yoshihiro H. Inoue","doi":"10.1111/gtc.13060","DOIUrl":"10.1111/gtc.13060","url":null,"abstract":"Drosophila mxcmbn1 mutant exhibits severe hyperplasia in larval hematopoietic tissue called the lymph glands (LGs). However, the malignant nature of these cells remains unknown. We aimed to identify if mxcmbn1 LG cells behave as malignant tumor cells and uncover the mechanism(s) underlying the malignancy of the mutant hemocytes. When mutant LG cells were allografted into normal adult abdomens, they continued to proliferate; however, normal LG cells did not proliferate. Mutant circulating hemocytes also attached to the larval central nervous system (CNS), where the basement membrane was disrupted. The mutant hemocytes displayed higher expression of matrix metalloproteinase (MMP) 1 and MMP2 and higher activation of the c-Jun N-terminal kinase (JNK) pathway than normal hemocytes. Depletion of MMPs or JNK mRNAs in LGs resulted in reduced numbers of hemocytes attached to the CNS, suggesting that the invasive phenotype involved elevated expression of MMPs via hyperactivation of the JNK pathway. Moreover, hemocytes with elongated filopodia and extra lamellipodia were frequently observed in the mutant hemolymph, which also depended on JNK signaling. Thus, the MMP upregulation and overextension of actin-based cell protrusions were also involved in hemocyte invasion in mxcmbn1 larvae. These findings contribute to the understanding of molecular mechanisms underlying mammalian leukemic invasion.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.13060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10056912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cdc7 kinase is required for postnatal brain development Cdc7激酶是出生后大脑发育所必需的。

IF 2.1 4区生物学

Genes to Cells Pub Date : 2023-08-16 DOI: 10.1111/gtc.13059

Karin Hori, Satoshi Yamazaki, Chiaki Ohtaka-Maruyama, Tomio Ono, Tomohiro Iguchi, Hisao Masai

{"title":"Cdc7 kinase is required for postnatal brain development","authors":"Karin Hori, Satoshi Yamazaki, Chiaki Ohtaka-Maruyama, Tomio Ono, Tomohiro Iguchi, Hisao Masai","doi":"10.1111/gtc.13059","DOIUrl":"10.1111/gtc.13059","url":null,"abstract":"The evolutionally conserved Cdc7 kinase plays crucial roles in initiation of DNA replication as well as in other chromosomal events. To examine the roles of Cdc7 in brain development, we have generated mice carrying Cdc7 knockout in neural stem cells by using Nestin-Cre. The Cdc7Fl/Fl NestinCre mice were born, but exhibited severe growth retardation and impaired postnatal brain development. These mice exhibited motor dysfunction within 9 days after birth and did not survive for more than 19 days. The cerebral cortical layer formation was impaired, although the cortical cell numbers were not altered in the mutant. In the cerebellum undergoing hypoplasia, granule cells (CGC) decreased in number in Cdc7Fl/F lNestinCre mice compared to the control at E15-18, suggesting that Cdc7 is required for DNA replication and cell proliferation of CGC at mid embryonic stage (before embryonic day 15). On the other hand, the Purkinje cell numbers were not altered but its layer formation was impaired in the mutant. These results indicate differential roles of Cdc7 in DNA replication/cell proliferation in brain. Furthermore, the defects of layer formation suggest a possibility that Cdc7 may play an additional role in cell migration during neural development.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10006539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Severe ethanol stress inhibits yeast proteasome activity at moderate temperatures but not at low temperatures 严重的乙醇胁迫在中等温度下抑制酵母蛋白酶体活性，但在低温下不抑制。

IF 2.1 4区生物学

Genes to Cells Pub Date : 2023-08-07 DOI: 10.1111/gtc.13058

Vo Thi Anh Nguyet, Ryoko Ando, Noboru Furutani, Shingo Izawa

引用次数: 0