Genes to Cells最新文献_第10页

The interaction between the import carrier Hikeshi and HSP70 is modulated by heat, facilitating the nuclear import of HSP70 under heat stress conditions 导入载体Hikeshi和HSP70之间的相互作用受热调节，在热胁迫条件下促进了HSP70的核导入。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2024-07-10 DOI: 10.1111/gtc.13145

Shingo Kose, Sakie Yoshioka, Yutaka Ogawa, Ai Watanabe, Naoko Imamoto

{"title":"The interaction between the import carrier Hikeshi and HSP70 is modulated by heat, facilitating the nuclear import of HSP70 under heat stress conditions","authors":"Shingo Kose, Sakie Yoshioka, Yutaka Ogawa, Ai Watanabe, Naoko Imamoto","doi":"10.1111/gtc.13145","DOIUrl":"10.1111/gtc.13145","url":null,"abstract":"Heat stress strongly triggers the nuclear localization of the molecular chaperone HSP70. Hikeshi functions as a unique nuclear import carrier of HSP70. However, how the nuclear import of HSP70 is activated in response to heat stress remains unclear. Here, we investigated the effects of heat on the nuclear import of HSP70. In vitro transport assays revealed that pretreatment of the test samples with heat facilitated the nuclear import of HSP70. Furthermore, binding of Hikeshi to HSP70 increased when temperatures rose. These results indicated that heat is one of the factors that activates the nuclear import of HSP70. Previous studies showed that the F97A mutation in Hikeshi in an extended loop induced an opening in the hydrophobic pocket and facilitated the translocation of Hikeshi through the nuclear pore complex. We found that nuclear accumulation of HSP70 occurred at a lower temperature in cells expressing the Hikeshi-F97A mutant than in cells expressing wild-type Hikeshi. Collectively, our results show that the movement of the extended loop may play an important role in the interaction of Hikeshi with both FG (phenylalanine-glycine)-nucleoporins and HSP70 in a temperature-dependent manner, resulting in the activation of nuclear import of HSP70 in response to heat stress.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"29 9","pages":"782-791"},"PeriodicalIF":1.3,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histone H3.3 chaperone HIRA renders stress-responsive genes poised for prospective lethal stresses in acquired tolerance 组蛋白 H3.3 合子 HIRA 使应激反应基因在获得性耐受中准备好应对未来的致命应激。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2024-07-08 DOI: 10.1111/gtc.13140

Yoshikazu Nagagaki, Yuji Kozakura, Theventhiran Mahandaran, Yukiko Fumoto, Ryuichiro Nakato, Katsuhiko Shirahige, Fuyuki Ishikawa

{"title":"Histone H3.3 chaperone HIRA renders stress-responsive genes poised for prospective lethal stresses in acquired tolerance","authors":"Yoshikazu Nagagaki, Yuji Kozakura, Theventhiran Mahandaran, Yukiko Fumoto, Ryuichiro Nakato, Katsuhiko Shirahige, Fuyuki Ishikawa","doi":"10.1111/gtc.13140","DOIUrl":"10.1111/gtc.13140","url":null,"abstract":"Appropriate responses to environmental challenges are imperative for the survival of all living organisms. Exposure to low-dose stresses is recognized to yield increased cellular fitness, a phenomenon termed hormesis. However, our molecular understanding of how cells respond to low-dose stress remains profoundly limited. Here we report that histone variant H3.3-specific chaperone, HIRA, is required for acquired tolerance, where low-dose heat stress exposure confers resistance to subsequent lethal heat stress. We found that human HIRA activates stress-responsive genes, including HSP70, by depositing histone H3.3 following low-dose stresses. These genes are also marked with histone H3 Lys-4 trimethylation and H3 Lys-9 acetylation, both active chromatin markers. Moreover, depletion of HIRA greatly diminished acquired tolerance, both in normal diploid fibroblasts and in HeLa cells. Collectively, our study revealed that HIRA is required for eliciting adaptive stress responses under environmental fluctuations and is a master regulator of stress tolerance.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"29 9","pages":"722-734"},"PeriodicalIF":1.3,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.13140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141558658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryo-EM structure and biochemical analyses of the nucleosome containing the cancer-associated histone H3 mutation E97K 含有癌症相关组蛋白 H3 突变 E97K 的核小体的低温电子显微镜结构和生化分析。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2024-07-07 DOI: 10.1111/gtc.13143

Tomoaki Kimura, Seiya Hirai, Tomoya Kujirai, Risa Fujita, Mitsuo Ogasawara, Haruhiko Ehara, Shun-ichi Sekine, Yoshimasa Takizawa, Hitoshi Kurumizaka

{"title":"Cryo-EM structure and biochemical analyses of the nucleosome containing the cancer-associated histone H3 mutation E97K","authors":"Tomoaki Kimura, Seiya Hirai, Tomoya Kujirai, Risa Fujita, Mitsuo Ogasawara, Haruhiko Ehara, Shun-ichi Sekine, Yoshimasa Takizawa, Hitoshi Kurumizaka","doi":"10.1111/gtc.13143","DOIUrl":"10.1111/gtc.13143","url":null,"abstract":"The Lys mutation of the canonical histone H3.1 Glu97 residue (H3E97K) is found in cancer cells. Previous biochemical analyses revealed that the nucleosome containing the H3E97K mutation is extremely unstable as compared to the wild-type nucleosome. However, the mechanism by which the H3E97K mutation causes nucleosome instability has not been clarified yet. In the present study, the cryo-electron microscopy structure of the nucleosome containing the H3E97K mutation revealed that the entry/exit DNA regions of the H3E97K nucleosome are disordered, probably by detachment of the nucleosomal DNA from the H3 N-terminal regions. This may change the intra-molecular amino acid interactions with the replaced H3 Lys97 residue, inducing structural distortion around the mutated position in the nucleosome. Consistent with the nucleosomal DNA end flexibility and the nucleosome instability, the H3E97K mutation exhibited reduced binding of linker histone H1 to the nucleosome, defective activation of PRC2 (the essential methyltransferase for facultative heterochromatin formation) with a poly-nucleosome, and enhanced nucleosome transcription by RNA polymerase II.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"29 9","pages":"769-781"},"PeriodicalIF":1.3,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.13143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatty acid preference for beta-oxidation in mitochondria of murine cultured astrocytes 小鼠培养的星形胶质细胞线粒体中脂肪酸的β-氧化偏好。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2024-07-04 DOI: 10.1111/gtc.13144

Laarni Grace Corales, Hitoshi Inada, Yuji Owada, Noriko Osumi

引用次数: 0

An N-terminal and ankyrin repeat domain interactome of Shank3 identifies the protein complex with the splicing regulator Nono in mice Shank3的N-末端和杏仁蛋白重复结构域相互作用组确定了与小鼠剪接调节因子Nono的蛋白质复合物。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2024-07-04 DOI: 10.1111/gtc.13142

Sayaka Okuzono, Fumihiko Fujii, Daiki Setoyama, Ryoji Taira, Yohei Shinmyo, Hiroki Kato, Keiji Masuda, Kousuke Yonemoto, Satoshi Akamine, Yuki Matsushita, Yoshitomo Motomura, Takeshi Sakurai, Hiroshi Kawasaki, Kihoon Han, Takahiro A. Kato, Hiroyuki Torisu, Dongchon Kang, Yusaku Nakabeppu, Shouichi Ohga, Yasunari Sakai

{"title":"An N-terminal and ankyrin repeat domain interactome of Shank3 identifies the protein complex with the splicing regulator Nono in mice","authors":"Sayaka Okuzono, Fumihiko Fujii, Daiki Setoyama, Ryoji Taira, Yohei Shinmyo, Hiroki Kato, Keiji Masuda, Kousuke Yonemoto, Satoshi Akamine, Yuki Matsushita, Yoshitomo Motomura, Takeshi Sakurai, Hiroshi Kawasaki, Kihoon Han, Takahiro A. Kato, Hiroyuki Torisu, Dongchon Kang, Yusaku Nakabeppu, Shouichi Ohga, Yasunari Sakai","doi":"10.1111/gtc.13142","DOIUrl":"10.1111/gtc.13142","url":null,"abstract":"An autism-associated gene Shank3 encodes multiple splicing isoforms, Shank3a-f. We have recently reported that Shank3a/b-knockout mice were more susceptible to kainic acid-induced seizures than wild-type mice at 4 weeks of age. Little is known, however, about how the N-terminal and ankyrin repeat domains (NT-Ank) of Shank3a/b regulate multiple molecular signals in the developing brain. To explore the functional roles of Shank3a/b, we performed a mass spectrometry-based proteomic search for proteins interacting with GFP-tagged NT-Ank. In this study, NT-Ank was predicted to form a variety of complexes with a total of 348 proteins, in which RNA-binding (n = 102), spliceosome (n = 22), and ribosome-associated molecules (n = 9) were significantly enriched. Among them, an X-linked intellectual disability-associated protein, Nono, was identified as a NT-Ank-binding protein. Coimmunoprecipitation assays validated the interaction of Shank3 with Nono in the mouse brain. In agreement with these data, the thalamus of Shank3a/b-knockout mice aberrantly expressed splicing isoforms of autism-associated genes, Nrxn1 and Eif4G1, before and after seizures with kainic acid treatment. These data indicate that Shank3 interacts with multiple RNA-binding proteins in the postnatal brain, thereby regulating the homeostatic expression of splicing isoforms for autism-associated genes after birth.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"29 9","pages":"746-756"},"PeriodicalIF":1.3,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.13142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141534213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein degradation by a component of the chaperonin-linked protease ClpP 伴侣蛋白连接蛋白酶 ClpP 的一种成分对蛋白质的降解作用。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2024-07-04 DOI: 10.1111/gtc.13141

Fumihiro Ishikawa, Michio Homma, Genzoh Tanabe, Takayuki Uchihashi

引用次数: 0

The CRK14 gene encoding a cysteine-rich receptor-like kinase is implicated in the regulation of global proliferative arrest in Arabidopsis thaliana 编码富半胱氨酸受体样激酶的 CRK14 基因与拟南芥全球增殖停滞的调控有关。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2024-06-28 DOI: 10.1111/gtc.13139

Sho Imai, Hikaru Hirozawa, Shingo Sugahara, Chisato Ishizaki, Mayu Higuchi, Yuma Matsushita, Takamasa Suzuki, Nobuyoshi Mochizuki, Akira Nagatani, Chiharu Ueguchi

{"title":"The CRK14 gene encoding a cysteine-rich receptor-like kinase is implicated in the regulation of global proliferative arrest in Arabidopsis thaliana","authors":"Sho Imai, Hikaru Hirozawa, Shingo Sugahara, Chisato Ishizaki, Mayu Higuchi, Yuma Matsushita, Takamasa Suzuki, Nobuyoshi Mochizuki, Akira Nagatani, Chiharu Ueguchi","doi":"10.1111/gtc.13139","DOIUrl":"10.1111/gtc.13139","url":null,"abstract":"Global proliferative arrest (GPA) is a phenomenon in monocarpic plants in which the activity of all aboveground meristems generally ceases in a nearly coordinated manner after the formation of a certain number of fruits. Despite the fact that GPA is a biologically and agriculturally important event, the underlying molecular mechanisms are not well understood. In this study, we attempted to elucidate the molecular mechanism of GPA regulation by identifying the gene responsible for the Arabidopsis mutant fireworks (fiw), causing an early GPA phenotype. Map-based cloning revealed that the fiw gene encodes CYSTEIN-RICH RECEPTOR-LIKE KINASE 14 (CRK14). Genetic analysis suggested that fiw is a missense, gain-of-function allele of CRK14. Since overexpression of the extracellular domain of CRK14 resulted in delayed GPA in the wild-type background, we concluded that CRK14 is involved in GPA regulation. Analysis of double mutants revealed that fiw acts downstream of or independently of the FRUITFULL-APETALA2 (AP2)/AP2-like pathway, which was previously reported as an age-dependent default pathway in GPA regulation. In addition, fiw is epistatic to clv with respect to GPA control. Furthermore, we found a negative effect on WUSCHEL expression in the fiw mutants. These results thus suggest the existence of a novel CRK14-dependent signaling pathway involved in GPA regulation.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"29 9","pages":"735-745"},"PeriodicalIF":1.3,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.13139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The hibernation promoting factor of Betaproteobacteria Comamonas testosteroni cannot induce 100S ribosome formation but stabilizes 70S ribosomal particles Betaproteobacteria Comamonas testosteroni 的冬眠促进因子不能诱导 100S 核糖体的形成，但能稳定 70S 核糖体颗粒。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2024-06-27 DOI: 10.1111/gtc.13137

Masami Ueta, Akira Wada, Chieko Wada

{"title":"The hibernation promoting factor of Betaproteobacteria Comamonas testosteroni cannot induce 100S ribosome formation but stabilizes 70S ribosomal particles","authors":"Masami Ueta, Akira Wada, Chieko Wada","doi":"10.1111/gtc.13137","DOIUrl":"10.1111/gtc.13137","url":null,"abstract":"Bacteria use several means to survive under stress conditions such as nutrient depletion. One such response is the formation of hibernating 100S ribosomes, which are translationally inactive 70S dimers. In Gammaproteobacteria (Enterobacterales), 100S ribosome formation requires ribosome modulation factor (RMF) and short hibernation promoting factor (HPF), whereas it is mediated by only long HPF in the majority of bacteria. Here, we investigated the role of HPFs of Comamonas testosteroni, which belongs to the Betaproteobacteria with common ancestor to the Gammaproteobacteria. C. testosteroni has two genes of HPF homologs of differing length (CtHPF-125 and CtHPF-119). CtHPF-125 was induced in the stationary phase, whereas CtHPF-119 conserved in many other Betaproteobacteria was not expressed in the culture conditions used here. Unlike short HPF and RMF, and long HPF, CtHPF-125 could not form 100S ribosome. We first constructed the deletion mutant of Cthpf-125 gene. When the deletion mutant grows in the stationary phase, 70S particles were degraded faster than in the wild strain. CtHPF-125 contributes to stabilizing the 70S ribosome. CtHPF-125 and CtHPF-119 both inhibited protein synthesis by transcription-translation in vitro. Our findings suggest that CtHPF-125 binds to ribosome, and stabilizes 70S ribosomes, inhibits translation without forming 100S ribosomes and supports prolonging life.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"29 8","pages":"613-634"},"PeriodicalIF":1.3,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis Mei5-Sae3复合体亚基Mei5在酵母减数分裂过程中Dmc1介导的重组中的突变分析

IF 1.3 4区生物学

Genes to Cells Pub Date : 2024-06-25 DOI: 10.1111/gtc.13138

Stephen Mwaniki, Priyanka Sawant, Osaretin P. Osemwenkhae, Yurika Fujita, Masaru Ito, Asako Furukohri, Akira Shinohara

引用次数: 0

Promoter characterization of relZ-bifunctional (pp)pGpp synthetase in mycobacteria 分枝杆菌中 relZ 双功能（pp）ppGpp 合成酶的启动子特征。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2024-06-24 DOI: 10.1111/gtc.13135

Neethu RS, Shubham Kumar Sinha, Sakshi Batra, Pavan Reddy Regatti, Kirtimaan Syal

{"title":"Promoter characterization of relZ-bifunctional (pp)pGpp synthetase in mycobacteria","authors":"Neethu RS, Shubham Kumar Sinha, Sakshi Batra, Pavan Reddy Regatti, Kirtimaan Syal","doi":"10.1111/gtc.13135","DOIUrl":"10.1111/gtc.13135","url":null,"abstract":"The second messenger guanosine 3',5'-bis(diphosphate)/guanosine tetraphosphate (ppGpp) and guanosine 3'-diphosphate 5'-triphosphate/guanosine pentaphosphate (pppGpp) ((p)ppGpp) has been shown to be crucial for the survival of mycobacteria under hostile conditions. Unexpectedly, deletion of primary (p)ppGpp synthetase-Rel did not completely diminish (p)ppGpp levels leading to the discovery of novel bifunctional enzyme-RelZ, which displayed guanosine 5'-monophosphate,3'-diphosphate (pGpp), ppGpp, and pppGpp ((pp)pGpp) synthesis and RNAseHII activity. What conditions does it express itself under, and does it work in concert with Rel? The regulation of its transcription and whether the Rel enzyme plays a role in such regulation remain unclear. In this article, we have studied relZ promoter and compared its activity with rel promoter in different growth conditions. We observed that the promoter activity of relZ was constitutive; it is weaker than rel promoter, lies within 200 bp upstream of translation-start site, and it increased under carbon starvation. Furthermore, the promoter activity of relZ was compromised in the rel-knockout strain in the stationary phase. Our study unveils the dynamic regulation of relZ promoter activity by SigA and SigB sigma factors in different growth phases in mycobacteria. Importantly, elucidating the regulatory network of RelZ would enable the development of the targeted interventions for treating mycobacterial infections.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"29 9","pages":"710-721"},"PeriodicalIF":1.3,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141456321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0