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Microsclerotia of dark septate endophytic fungi from Paleogene and Neogene of Poland: fossil record and palaeoenvironmental significance of root fungal endophytes 波兰古近纪和新近纪暗隔内生真菌的微核:根真菌内生真菌的化石记录及其古环境意义
IF 2.9 3区 生物学
Fungal biology Pub Date : 2025-05-13 DOI: 10.1016/j.funbio.2025.101594
Grzegorz Worobiec , Elżbieta Worobiec , Marek Widera , Wojciech Granoszewski
{"title":"Microsclerotia of dark septate endophytic fungi from Paleogene and Neogene of Poland: fossil record and palaeoenvironmental significance of root fungal endophytes","authors":"Grzegorz Worobiec ,&nbsp;Elżbieta Worobiec ,&nbsp;Marek Widera ,&nbsp;Wojciech Granoszewski","doi":"10.1016/j.funbio.2025.101594","DOIUrl":"10.1016/j.funbio.2025.101594","url":null,"abstract":"<div><div>Remains of microsclerotia morphologically corresponding to those of modern dark septate endophytic (DSE) fungi were found during palynological studies of some Oligocene and Miocene sites from Poland. Fossil microsclerotia, mostly represented by leaf-like morphotypes were morphologically diversified, but there was no possibility to match the fossil morphotypes with modern fungal DSE taxa forming microsclerotia in roots. Reliable fossils of microsclerotia of dark septate endophytic fungi date back to the Eocene and show continuous presence till recent times. They confirm the coexistence of dark septate endophytic fungi with plants at least from the beginning of the early Cenozoic. Pre-Cenozoic fossils of presumed DSE microsclerotia were considered doubtful. The presence of microsclerotia in continental deposits points to dense vegetation in the past, and in marine deposits, they indicate terrigenous flux that originated from coastal vegetation.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101594"},"PeriodicalIF":2.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144114987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell wall characteristics of Thielavia terrestris dispersed and pelleted mycelia 地蓟分散和球化菌丝的细胞壁特性
IF 2.9 3区 生物学
Fungal biology Pub Date : 2025-05-09 DOI: 10.1016/j.funbio.2025.101593
Olena Gromozova , Mara Grube , Peter Lytvyn , Maksym Kharchuk , Marina Fomina
{"title":"Cell wall characteristics of Thielavia terrestris dispersed and pelleted mycelia","authors":"Olena Gromozova ,&nbsp;Mara Grube ,&nbsp;Peter Lytvyn ,&nbsp;Maksym Kharchuk ,&nbsp;Marina Fomina","doi":"10.1016/j.funbio.2025.101593","DOIUrl":"10.1016/j.funbio.2025.101593","url":null,"abstract":"<div><div>In submerged fermentation of filamentous fungi, pelleted and dispersed types of growth are the two main morphological forms largely affecting the productivity of industrial processes. Despite the immense importance of fungal biotechnology various aspects of the biology of these two growth forms have been rarely studied. Whether there are any differences in architecture and properties of hyphal cell wall between pelleted and dispersed mycelia of the same non-genetically modified fungus was unknown. This comparative study characterized the cell wall structure and properties of pelleted and dispersed forms of <em>Thielavia terrestris</em> growth. Infrared spectroscopy revealed that the hyphal cell wall of pellets, in contrast to dispersed mycelium, contained considerably more chitin (22 % and 1–2 % of dry weight [dw], respectively), glucans (28 % and 10 % dw), and lipids (8 % and 6 % dw). The dispersed form was characterised by much higher content of phosphate-rich polysaccharide (dispersed vs pelleted: 23 % and 1 % dw) and protein (55 % and 40 % dw, respectively). TEM-microscopy showed the thicker cell wall of the dispersed form, which did not manifest the third well-defined electron-dense smooth outer layer present in pellets. The cell wall of the pelleted form was less susceptible to mechanical and enzymatic destruction and demonstrated in AFM hardness tests 5–10 times greater nano-hardness than the dispersed form. Thus, the cell wall structure and properties are very different in the pelleted than the dispersed growth forms, where the narrower and chitin/glucans-richer cell wall of the pelleted form provides more effective protection from mechanical and enzymatic damages.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101593"},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Elemental uptake and accumulation by wood decay fungi in relation to their nutrition strategy, rot type and mineral profile of the colonized wood 木材腐烂真菌对元素的吸收和积累与定殖木材的营养策略、腐烂类型和矿物特征有关
IF 2.9 3区 生物学
Fungal biology Pub Date : 2025-05-08 DOI: 10.1016/j.funbio.2025.101592
Magdalena Krzesłowska , Patrycja Mleczek , Anna Budka , Marek Siwulski , Sylwia Budzyńska , Przemysław Niedzielski , Július Árvay , Mirosław Mleczek
{"title":"Elemental uptake and accumulation by wood decay fungi in relation to their nutrition strategy, rot type and mineral profile of the colonized wood","authors":"Magdalena Krzesłowska ,&nbsp;Patrycja Mleczek ,&nbsp;Anna Budka ,&nbsp;Marek Siwulski ,&nbsp;Sylwia Budzyńska ,&nbsp;Przemysław Niedzielski ,&nbsp;Július Árvay ,&nbsp;Mirosław Mleczek","doi":"10.1016/j.funbio.2025.101592","DOIUrl":"10.1016/j.funbio.2025.101592","url":null,"abstract":"<div><div>The uptake and accumulation of essential and toxic elements by wood decay fungi (WDF) are complex. We examined the mineral profile of 13 WDF colonizing coniferous and deciduous trees. To illustrate the role of nutritional strategies and rot type in selected elements uptake and accumulation, four fungi species differing in their nutrition strategy and the type of decay, colonizing only conifers were selected: two white rot: saprotrophic <em>Pseudohydnum gelatinosum,</em> and parasitic <em>Phellinus pini</em> and two brown rot, saprotrophic/parasitic <em>Phaeolus schweinitzii</em> and saprotrophic <em>Tapinella atrotomentosa</em>. The obtained results revealed that the concentration of most selected major elements (K, Mg, Na, P) in the sporocarps of the two saprotrophic WDF species was significantly higher than in the parasitic or saprotrophic/parasitic ones and that the concentration in the colonized wood located under the sporocarp projecting from the trunk or root. Furthermore, it was found that white rot fungi, independent of their nutritional strategy, accumulated significantly more of most selected minor (B, Cu, Fe, Ni, Zn) and toxic (Al, As, Cd, Pb, Tl) elements in their sporocarps than brown rot species. These last two rules concerned WDF species colonizing coniferous trees but also included WDF species growing on deciduous trees.</div><div>Hence, obtained, novel results of this paper demonstrated that the mineral nutrition of WDF, mainly colonizing coniferous trees, may be influenced by (1) fungus nutrition strategy, (2) the type of decay - involved in the preferential decomposition of specific cell wall components and fungus demand for given element, involved in wood CWs decomposition strategy and (3) to a lesser extent by the elements concentration in the wood samples.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101592"},"PeriodicalIF":2.9,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143936183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Celebrating the fifth edition of the International Symposium on Fungal Stress – ISFUS, a decade after its 2014 debut 庆祝第五届国际真菌胁迫研讨会- ISFUS,在2014年首次亮相十年后
IF 2.9 3区 生物学
Fungal biology Pub Date : 2025-05-06 DOI: 10.1016/j.funbio.2025.101590
Alene Alder-Rangel , Amanda E.A. Rangel , Arturo Casadevall , Asiya Gusa , Chaoyang Xue , Charles M. Boone , Chris Todd Hittinger , Claudio A. Masuda , Consuelo Olivares-Yañez , Deborah Bell-Pedersen , Erica J. Washington , Gerhard Braus , Guilhem Janbon , István Pócsi , Jason E. Stajich , Jay C. Dunlap , Joan W. Bennett , Joseph Heitman , Ling Lu , Lucia Landi , Drauzio E.N. Rangel
{"title":"Celebrating the fifth edition of the International Symposium on Fungal Stress – ISFUS, a decade after its 2014 debut","authors":"Alene Alder-Rangel ,&nbsp;Amanda E.A. Rangel ,&nbsp;Arturo Casadevall ,&nbsp;Asiya Gusa ,&nbsp;Chaoyang Xue ,&nbsp;Charles M. Boone ,&nbsp;Chris Todd Hittinger ,&nbsp;Claudio A. Masuda ,&nbsp;Consuelo Olivares-Yañez ,&nbsp;Deborah Bell-Pedersen ,&nbsp;Erica J. Washington ,&nbsp;Gerhard Braus ,&nbsp;Guilhem Janbon ,&nbsp;István Pócsi ,&nbsp;Jason E. Stajich ,&nbsp;Jay C. Dunlap ,&nbsp;Joan W. Bennett ,&nbsp;Joseph Heitman ,&nbsp;Ling Lu ,&nbsp;Lucia Landi ,&nbsp;Drauzio E.N. Rangel","doi":"10.1016/j.funbio.2025.101590","DOIUrl":"10.1016/j.funbio.2025.101590","url":null,"abstract":"<div><div>The Fifth International Symposium on Fungal Stress (ISFUS) brought together in Brazil many of the leaders in the field of fungal stress responses, from fourteen countries, for four days of outstanding science ranging from basic research to studies with agricultural, medical, industrial, and environmental significance. In addition to the excellent oral and poster presentations, the Symposium organisers ensured that all participants had ample opportunity to engage, socialise, and network to exchange ideas and share research. The conference was enhanced by the world-class venue near Iguazu Falls, probably the greatest natural phenomenon in South America.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101590"},"PeriodicalIF":2.9,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biological-degradation, lignin performance and physical-chemical characteristics of historical wood in an ancient tomb 古墓中历史木材的生物降解、木质素性能及理化特性
IF 2.9 3区 生物学
Fungal biology Pub Date : 2025-04-22 DOI: 10.1016/j.funbio.2025.101588
Akbar Mastouri , Davood Efhamisisi , Martin Lexa , Reza Oladi , Alireza Gholinejad-Pirbazari , Hossein Torabi , Aleš Zeidler , Mariaenrica Frigione
{"title":"Biological-degradation, lignin performance and physical-chemical characteristics of historical wood in an ancient tomb","authors":"Akbar Mastouri ,&nbsp;Davood Efhamisisi ,&nbsp;Martin Lexa ,&nbsp;Reza Oladi ,&nbsp;Alireza Gholinejad-Pirbazari ,&nbsp;Hossein Torabi ,&nbsp;Aleš Zeidler ,&nbsp;Mariaenrica Frigione","doi":"10.1016/j.funbio.2025.101588","DOIUrl":"10.1016/j.funbio.2025.101588","url":null,"abstract":"<div><div>Biodeterioration assessments of archaeological woods, also comparisons with recent wood, provides an effective strategy for its conservation in cultural-heritage. This research aimed to analyze some wooden structures of an ancient tomb, situated in West-Azerbaijan province of Iran adjacent to Lake-Urmia. The anatomical characteristics of historical wood (HW) was investigated to identify species and trace xylem micro-morphological variations in detail. Chemical-changes of deteriorated wood were examined using X-ray diffraction (XRD) and ATR-FTIR spectroscopy, and its wood–water interactions were compared with the recent new-wood. Macro/micro-morphological and mycological assessment of historical poplar wood (<em>Populus alba</em>) confirmed signs of soft-rot fungi, and recent attacks by wood–boring (Xylophagous) insects probably due to local climate-change. Type-I microcavities and advanced soft-rot decay caused cell-walls depletion or deformation, which was visually observed in HW by discoloration and transverse-cracks. Based on Optical- and SEM-microscopy, lignin preservation was observed in the middle-lamella and especially the vessels rich in guaiacyl-lignin (G-type) units. However, the fiber walls were susceptible to fungal degradation due to syringyl-lignin (S-type). Histochemical changes increased the porosity, hygroscopicity, and especially water-absorption (1h–720h) of HW compared to the corresponding new-wood, introducing the severity of damage and treatability criteria for conservationists. Lignin increment, loss of cellulose-crystallinity in XRD (70.5 to 59.9 %) and FTIR ratios (1280/1200, 1317/1336), and also preferential-degradation of polysaccharides were confirmed through chemical analyses of soft-rot decayed wood. These findings, in addition to helping monitor potential risks of wooden artifacts, especially non-durable species, facilitate preventive and protective management of archaeological-heritage in both wet and dry environments.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 4","pages":"Article 101588"},"PeriodicalIF":2.9,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143867827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Epidemiology and genetic characterization of Alternaria alternata causing leaf spot in Fragaria × ananassa 黄花菜叶斑病的流行病学及遗传特征
IF 2.9 3区 生物学
Fungal biology Pub Date : 2025-04-22 DOI: 10.1016/j.funbio.2025.101589
Nasir Mehmood , Abid Riaz , Salman Ghuffar , Sadaf Anwaar , Nyla Jabeen , Isha Shaheen , Muhammad Qasim , Salma Saeed Khan , Muhammad Rauf , Tauseef Anwar , Huma Qureshi , Ghadah Salim Abusalim , Wajid Zaman , Mohammad Javed Ansari , Rashid Iqbal
{"title":"Epidemiology and genetic characterization of Alternaria alternata causing leaf spot in Fragaria × ananassa","authors":"Nasir Mehmood ,&nbsp;Abid Riaz ,&nbsp;Salman Ghuffar ,&nbsp;Sadaf Anwaar ,&nbsp;Nyla Jabeen ,&nbsp;Isha Shaheen ,&nbsp;Muhammad Qasim ,&nbsp;Salma Saeed Khan ,&nbsp;Muhammad Rauf ,&nbsp;Tauseef Anwar ,&nbsp;Huma Qureshi ,&nbsp;Ghadah Salim Abusalim ,&nbsp;Wajid Zaman ,&nbsp;Mohammad Javed Ansari ,&nbsp;Rashid Iqbal","doi":"10.1016/j.funbio.2025.101589","DOIUrl":"10.1016/j.funbio.2025.101589","url":null,"abstract":"<div><div>Alternaria leaf spot (ALS), caused by <em>Alternaria alternata</em>, is a major disease threatening strawberry (<em>Fragaria × ananassa</em>) production globally, including in Pakistan. This study investigated the incidence and prevalence of ALS in key strawberry-producing regions of Pakistan and characterized the pathogen using morphological and molecular techniques. Surveys were conducted during the 2014–2015 and 2015–2016 seasons across 182 farms in Punjab, Khyber Pakhtunkhwa, and Islamabad. Disease prevalence was 100% across all regions, with incidence rates ranging from 17.25% in cooler Islamabad to 55% in Mardan, KPK. Pathogenicity tests confirmed <em>A. alternata</em> as the causal agent. Morphological traits and sequencing of the ITS and endoPG genes further validated its identity. Phylogenetic analysis showed close genetic relatedness to known <em>A. alternata</em> isolates. This is the first comprehensive report of ALS in strawberries in Pakistan, confirming <em>A. alternata</em> as the primary pathogen. The widespread occurrence and high incidence highlight the urgent need for effective control measures. Integrated disease management, including resistant cultivars and targeted fungicide use, is strongly recommended. Further research should investigate environmental factors influencing disease spread and severity to support long-term sustainable management of ALS in strawberry cultivation.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 4","pages":"Article 101589"},"PeriodicalIF":2.9,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fungal microbiota in peritoneal dialysis effluent related peritonitis patients by amplicon sequencing of internal transcribed spacer based on Oxford nanopore Technologies 基于牛津纳米孔技术的内部转录间隔子扩增子测序研究腹膜透析出水相关性腹膜炎患者真菌微生物群
IF 2.9 3区 生物学
Fungal biology Pub Date : 2025-04-18 DOI: 10.1016/j.funbio.2025.101587
Suthida Visedthorn , Suwalak Chitcharoen , Pavit Klomkliew , Vorthon Sawaswong , Pavaret Sivapornnukul , Prangwalai Chanchaem , Thunvarat Saejew , Preeyarat Pavatung , Talerngsak Kanjanabuch , Sunchai Payungporn
{"title":"Fungal microbiota in peritoneal dialysis effluent related peritonitis patients by amplicon sequencing of internal transcribed spacer based on Oxford nanopore Technologies","authors":"Suthida Visedthorn ,&nbsp;Suwalak Chitcharoen ,&nbsp;Pavit Klomkliew ,&nbsp;Vorthon Sawaswong ,&nbsp;Pavaret Sivapornnukul ,&nbsp;Prangwalai Chanchaem ,&nbsp;Thunvarat Saejew ,&nbsp;Preeyarat Pavatung ,&nbsp;Talerngsak Kanjanabuch ,&nbsp;Sunchai Payungporn","doi":"10.1016/j.funbio.2025.101587","DOIUrl":"10.1016/j.funbio.2025.101587","url":null,"abstract":"<div><div>Fungal peritonitis (FP) is a rare and severe complication resulting from fungal infections of the peritoneum. It has recently garnered increased attention due to rising both incidence and potential mortality. Early recognition and rapid intervention are essential for improving patient outcomes, as untreated FP can progress to sepsis and multi-organ failure if not treated appropriately. Culture-negative fungal peritonitis is commonly observed, and its presence should be further considered. This study aimed to utilize Oxford Nanopore Technologies (ONT) sequencing to investigate the fungal profiles present in peritoneal dialysis effluent (PDE) from patients experiencing PD-related peritonitis, based on internal transcribed spacer (ITS). The results indicated that the ONT was able to identify fungal taxa in 69 out of 104 samples (66.3 %), which included mixed organisms. The predominant phyla identified in the PDE sample were Basidiomycota, followed by Ascomycota and Glomeromycota. Notably, <em>Wallemia</em> was the most frequently detected fungal genus suggesting the fungal thrive in moist environments and can contaminate dialysis system. This observation suggests a possible association with opportunistic infections in immunocompromised individuals, particularly those undergoing peritoneal dialysis. In conclusion, ONT offers a promising approach for fungal taxonomic classification, even though the DNA extracted from the samples was of low abundance.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 4","pages":"Article 101587"},"PeriodicalIF":2.9,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Systematic evaluation of fluoride quantification methods identifies an appropriate measurement for studying fungal defluorination of per- and Polyfluoroalkyl substances 对氟定量方法的系统评价确定了一种合适的测量方法,用于研究全氟烷基和多氟烷基物质的真菌脱氟
IF 2.9 3区 生物学
Fungal biology Pub Date : 2025-04-15 DOI: 10.1016/j.funbio.2025.101586
Charles Ayers, Jiwei Zhang
{"title":"Systematic evaluation of fluoride quantification methods identifies an appropriate measurement for studying fungal defluorination of per- and Polyfluoroalkyl substances","authors":"Charles Ayers,&nbsp;Jiwei Zhang","doi":"10.1016/j.funbio.2025.101586","DOIUrl":"10.1016/j.funbio.2025.101586","url":null,"abstract":"<div><div>The large-scale manufacturing and disposal of Per- and Polyfluoroalkyl substances (PFASs) are causing global concerns, and investigations are needed to understand their ecological impacts. Saprotrophic fungi are dominant nutrient recyclers in ecosystems, while their roles in transforming PFAS remain largely untapped. Particularly, there is a lack of appropriate means to rapidly measure fluoride anions released by fungal cultures to understand their defluorination (deF) capacities and mechanisms. In this research, we systematically evaluated three prevalent means for quantifying free fluoride anions under cultural conditions of wood decay fungal species. Two spectrophotometric measurings, including one based on the fluoride-catalyzed kinetochromic reaction of Xylenol Orange-Zirconium (XO-Zr<sup>4+</sup>) and another one based on Alizarin-Lanthanum-Fluoride (Al–La<sup>3+</sup>-F<sup>-</sup>) ternary complexone reaction, were successfully adapted to a high-throughput micro-well setup for detecting micromolar-level fluoride, but essential fungal media components pose significant interference to both assays. The third method, relying on the potentiometer and fluoride ion-selective electrode (F-ISE), demonstrated a strong capacity for anti-interferences of fungal nutrients, metabolites, and PFAS, leaving it a most appropriate method to study fungal deF. With F-ISE, we further demonstrated a deF test using a model PFAS, 4,4,4-trifluoro-3-(trifluoromethyl) crotonic acid, and a wood rot fungus <em>Trametes versicolor</em>. Together, our research identifies a reliable fluoride quantification method that can allow assessing fungal cultures for their PFAS-defluorinating phenotypes.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 4","pages":"Article 101586"},"PeriodicalIF":2.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fusarium graminearum and zearalenone in wheat: A water activity–temperature model 小麦赤霉病菌与玉米赤霉烯酮:水分活度-温度模型
IF 2.9 3区 生物学
Fungal biology Pub Date : 2025-04-11 DOI: 10.1016/j.funbio.2025.101572
B. Ingram , S. Marin , E. Kiaitsi , N. Magan , C. Verheecke-Vaessen , C. Cervini , F. Rubio-Lopez , E. Garcia-Cela
{"title":"Fusarium graminearum and zearalenone in wheat: A water activity–temperature model","authors":"B. Ingram ,&nbsp;S. Marin ,&nbsp;E. Kiaitsi ,&nbsp;N. Magan ,&nbsp;C. Verheecke-Vaessen ,&nbsp;C. Cervini ,&nbsp;F. Rubio-Lopez ,&nbsp;E. Garcia-Cela","doi":"10.1016/j.funbio.2025.101572","DOIUrl":"10.1016/j.funbio.2025.101572","url":null,"abstract":"<div><div>Zearalenone (ZEN) is a nonsteroidal estrogenic mycotoxin produced primarily by <em>Fusarium graminearum</em>, posing significant threats to agricultural grain production. When ZEN levels exceed regulatory limits, grains face rejection, and its harmful effects on the female reproductive system raise health concerns. Despite its importance, there is a lack of information on the ecophysiological conditions that promote <em>F. graminearum</em> colonisation and ZEN production in wheat grains. This study aimed to develop and validate predictive models for the growth of <em>F.graminearum</em> and ZEN accumulation in wheat. For this purpose, two strains isolated from wheat were inoculated in agar wheat-based medium supplemented with glycerol to adjust the water activity (a<sub>w</sub>) to five different values of 0.88, 0.91, 0.94, 0.97 and 0.99. The cultures were incubated at 4, 6, 8.5, 15, 20, 25, 30 and 35 °C, the colony growth was measured daily, and ZEN accumulation assessed at day 10, 20 and 30. To analyse the growth kinetics of <em>F. graminearum</em>, the fungal growth rate (μ) and lag time (λ) were calculated, applying the Cardinal/Rosso, Davey, and Gibson models. These techniques, commonly used in secondary modelling, were enhanced through variable transformation, with the square root transformation yielding optimal results in the Cardinal models. The outcome showed probabilistic model accuracy for growth ranging 65–79 % and ZEN production ranging 45–77 % on internal and external data set. Optimum temperature for ZEN production was 25–30 °C in media and wheat. In wheat, a higher a<sub>W</sub> was required for both growing (0.92 a<sub>w</sub>) and ZEN production compared to media (0.90 a<sub>w</sub>). Probabilities of growth over 80 % were predicted in the range of 0.90–0.95 a<sub>w</sub> at 16–34 °C after 30 days. In conclusion, to avoid mycotoxin contamination in wheat an a<sub>w</sub> &lt; 0.89 should be maintained, and temperatures in the range 18–31 °C should be avoided (P &lt; 0.5). The integration of predictive models into decision support systems could assist farmers in identifying pre-harvest contamination risks and in optimising harvesting and drying practices to minimise post-harvest contamination. This study highlights the importance of understanding the ecophysiological profiles of mycotoxigenic species like <em>F. graminearum</em> to mitigate contamination risks and optimise storage conditions in wheat.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 4","pages":"Article 101572"},"PeriodicalIF":2.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143839113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Physiological stress tolerance responses of the dung decomposer fungus Mucor circinelloides 粪便分解者真菌毛霉的生理抗逆性响应
IF 2.9 3区 生物学
Fungal biology Pub Date : 2025-04-10 DOI: 10.1016/j.funbio.2025.101575
Mavis A. Acheampong , Breno Pupin , Luciana P. Dias , Maiara P. Santos , Luana O. Bernardes , Michele S. Pinheiro , Paulo C. Ferreira , Aram Mikaelyan , Humberto R. Medina , Alene Alder-Rangel , Drauzio E.N. Rangel
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