Physiological stress tolerance responses of the dung decomposer fungus Mucor circinelloides

IF 2.9 3区生物学 Q2 MYCOLOGY

Fungal biology Pub Date : 2025-04-10 DOI:10.1016/j.funbio.2025.101575

Mavis A. Acheampong , Breno Pupin , Luciana P. Dias , Maiara P. Santos , Luana O. Bernardes , Michele S. Pinheiro , Paulo C. Ferreira , Aram Mikaelyan , Humberto R. Medina , Alene Alder-Rangel , Drauzio E.N. Rangel

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Abstract

The Mucor circinelloides complex, a group of dung-dwelling fungi, is essential for nutrient cycling and organic matter breakdown. Despite their ecological significance, variations in germination rates and stress tolerance among isolates are not well understood. This study examined four isolates—Cicarelli, Doc Beaver, Papulsa, and Zaina—to assess their germination on various media, including potato dextrose agar (PDA), Czapek (CZA), Sabouraud dextrose agar (SDA), Emerson (EM), and minimal media (MM). The results showed notable differences in germination rates, with CZA and EM promoting the fastest growth. The study also analysed sporangiospore tolerance to UV-B radiation and high temperatures (47 °C), with findings highlighting the role of growth media in stress resistance. Sporangiospores from Cicarelli, Doc Beaver, and Papulsa grown on PDA exhibited greater UV-B tolerance than those on CZA. Conversely, sporangiospores produced on CZA were more heat tolerant than those on PDA. The 12-h survival curve for the Zaina isolate reinforced these findings, showing similar trends in UV-B resilience and heat tolerance. This research demonstrates how growth media influence the environmental stress responses in M. circinelloides, providing insights into the adaptive potential of these fungi.

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粪便分解者真菌毛霉的生理抗逆性响应

环状毛霉复合体是一组居住在粪便中的真菌，对养分循环和有机物分解至关重要。尽管具有重要的生态意义，但不同菌株之间的发芽率和胁迫耐受性的差异尚不清楚。本研究检测了4个分离株cicarelli、Doc Beaver、Papulsa和zaina，以评估它们在不同培养基上的萌发情况，包括马铃薯葡萄糖琼脂（PDA）、Czapek （CZA）、Sabouraud葡萄糖琼脂（SDA）、Emerson （EM）和minimal培养基（MM）。结果表明，种子发芽率差异显著，CZA和EM对种子生长的促进作用最大。该研究还分析了孢子对UV-B辐射和高温（47°C）的耐受性，研究结果突出了生长介质在抗逆性中的作用。在PDA上生长的Cicarelli、Doc Beaver和Papulsa孢子对UV-B的耐受性优于CZA。相反，在CZA上产生的孢子管孢子比在PDA上产生的更耐热。Zaina分离物的12小时存活曲线强化了这些发现，显示出类似的UV-B抗逆性和耐热性趋势。本研究展示了生长介质如何影响环孢霉的环境胁迫反应，为这些真菌的适应潜力提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fungal biology MYCOLOGY-

CiteScore

5.80

自引率

4.00%

发文量

审稿时长

49 days

期刊介绍： Fungal Biology publishes original contributions in all fields of basic and applied research involving fungi and fungus-like organisms (including oomycetes and slime moulds). Areas of investigation include biodeterioration, biotechnology, cell and developmental biology, ecology, evolution, genetics, geomycology, medical mycology, mutualistic interactions (including lichens and mycorrhizas), physiology, plant pathology, secondary metabolites, and taxonomy and systematics. Submissions on experimental methods are also welcomed. Priority is given to contributions likely to be of interest to a wide international audience.