Fungal biology最新文献

{"title":"Transcriptome analysis reveals the molecular mechanisms of the 8-day infection cycle of Ecytonucleospora hepatopenaei in Litopenaeus vannamei and the host's molecular responses","authors":"Yan Rong ,&nbsp;Yi Qiao ,&nbsp;Tingyue Liu ,&nbsp;Xiaohui Cao ,&nbsp;Ge Jiang ,&nbsp;Jie Cheng ,&nbsp;Huiwen Jiang ,&nbsp;Xianping Fan ,&nbsp;Hui Shen","doi":"10.1016/j.funbio.2025.101613","DOIUrl":"10.1016/j.funbio.2025.101613","url":null,"abstract":"<div><div><em>Ecytonucleospora hepatopenaei</em> (EHP), an emerging microsporidian, has caused significant economic losses to the Asian aquaculture industry. This study investigates the infection dynamics of EHP through deep transcriptomic sequencing of infected shrimp samples at different time points. The analysis revealed distinct periodic changes in metabolism, immune response, and DNA repair during EHP infection. Shrimp rapidly mobilize energy metabolism and activate immune pathways in the early stage (day 2), followed by immune suppression and metabolic regulation in the mid-stage (days 4–6), and enhanced immune responses by day 8. Trend analysis supports an 8-day infection cycle, with periodic changes in gene expression profiles, especially in energy metabolism, protein synthesis and degradation, and immune responses. Host gene expression displays a phased response aligning with EHP proliferation and its life cycle. EHP's gene expression also shows periodicity, with upregulation of proliferation-associated genes on days 4 and 10. Weighted Gene Co-expression Network Analysis (WGCNA) identified key gene modules related to metabolic adaptation and immune suppression, indicating EHP's adaptation to the host environment. The study reveals a cyclical pattern of interaction between EHP and its host, providing insights into EHP's pathogenic mechanisms.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101613"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sterol aminoacylation regulates conidiation and sclerotia formation in Aspergillus oryzae","authors":"Daisuke Yokokawa ,&nbsp;Shunsuke Tatematsu ,&nbsp;Makoto Imai ,&nbsp;Yoshihiro Takara ,&nbsp;Yusuke Saga ,&nbsp;Frédéric Fischer ,&nbsp;Hubert D. Becker ,&nbsp;Harushi Nakajima ,&nbsp;Tetsuo Kushiro","doi":"10.1016/j.funbio.2025.101609","DOIUrl":"10.1016/j.funbio.2025.101609","url":null,"abstract":"<div><div>Ergosterol is a major fungal membrane component that contributes to membrane functions. We recently discovered novel ergosterol derivatives conjugated with amino acids, namely two aminoacylated ergosterols (Erg-aa), 1-ergosteryl-L-aspartate (Erg-Asp) and 1-ergosteryl-glycine (Erg-Gly), and the synthase involved (ErdS and ErgS, respectively). These enzymes are widely conserved in fungi belonging to the Dikarya subkingdom. However, the physiological role of Erg-aa remains unknown. Here, we report that Erg-aa are involved in conidiation and sclerotia formation in <em>Aspergillus oryzae</em>. The deletion of <em>AoerdS</em> or <em>AoergS</em> led to the reduction of conidia, accompanied by the downregulation of conidiation-regulating genes. In contrast, these deletants formed sclerotia considerably. <em>AoerdS</em> inducible strains also decreased conidia and promoted sclerotia formation under <em>AoerdS</em> suppressing condition, whereas the opposite morphology was observed under <em>AoerdS</em> inductive condition. We also investigated the effects of synthetic Erg-aa on fungal growth. Externally added Erg-Asp slightly promoted conidiation but did not affect sclerotia formation. On the other hand, Erg-Gly promoted the elongation of aerial hyphae and reduced both conidia and sclerotia. Finally, the deletion of <em>AoerdS</em> or <em>AoergS</em> altered susceptibility to ergosterol associating agents, indicating that ergosterol aminoacylation may change the physicochemical properties of ergosterol. Our findings expand the biological role of sterol modification in fungi.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101609"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144229903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential profiling of viable fungi in the gut of Penaeus vannamei cultured in brackishwater ponds with high protein, low protein and azolla-based diets","authors":"N.S. Sudheer ,&nbsp;I.F. Biju ,&nbsp;L. Priyadarshini ,&nbsp;D. De ,&nbsp;T.K. Ghoshal ,&nbsp;Babita Mandal ,&nbsp;S. Das","doi":"10.1016/j.funbio.2025.101595","DOIUrl":"10.1016/j.funbio.2025.101595","url":null,"abstract":"<div><div>An understanding of the gut microflora of an organism provides vital information that could be used in developing strategies for health management. In this view, a differential profiling of fungi in the gut of <em>Penaeus vannamei</em> fed on high protein, low protein and azolla-based diets are carried out. Shrimp samples were collected from brackish water ponds maintained on various dietary changes and fungus species were isolated and identified. A total of 43 fungi isolates belonging to 29 species, 2 phyla, 8 classes, 11 orders, 22 families and 23 genera ware identified. Among the identified species 74.41 % are represented by phylum Ascomycota and 25.60 % of the species are represented by Basidiomycota. <em>Sakaguchia oryzae</em> (9 %) and <em>Parasarocladium breve</em> (9 %) were the dominant species. The data indicate that higher fungal diversity was present in low protein administered groups followed by high protein feed with a slight difference. The lowest fungal diversity was in the azolla-based diet administered group. <em>P. breve</em> and <em>Penicillium osxalicum</em> were abundant in the high protein feed administered groups. <em>S. oryzae</em> and <em>Acremonium brachypenium</em> ware abundant in low protein and azolla-based feed groups, respectively. The genera Acremonium (4.3 %) was found common in all tested diet administered groups. The results indicated that dietary changes may influence the fungal diversity in the gut of <em>P. vannamei</em>. Our study provides valuable information on the gut mycobiota of <em>P. vannamei</em> cultured on dietary changes through employing modern data analysis and visualization tools, which is essential for developing an effective management strategy to improve shrimp culture.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101595"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the impact of apocarotenoids on pathogenic Fusarium oxysporum f.sp. lini and endophytic Fo47 strains","authors":"Yelyzaveta Kochneva ,&nbsp;Marta Burgberger-Stawarz ,&nbsp;Aleksandra Boba ,&nbsp;Marta Preisner ,&nbsp;Justyna Mierziak-Derecka ,&nbsp;Anna Kulma","doi":"10.1016/j.funbio.2025.101591","DOIUrl":"10.1016/j.funbio.2025.101591","url":null,"abstract":"<div><div>The <em>Fusarium oxysporum</em> species complex (FOSC) contains highly specific plant pathogens and some nonpathogenic strains, such as <em>Fo47</em>. Our work concentrated on <em>F. oxysporum</em> f.sp. <em>lini</em> (<em>Foln</em>), the specific flax pathogen and the endophytic strain <em>F. oxysporum</em> 47 (<em>Fo47</em>), which is suggested to have a protective effect on flax against pathogens. We investigated the effects of apocarotenoids, including ionones and abscisic acid (ABA), on the growth and development of these fungal strains, assessing the potential fungicidal properties of these compounds and comparing the responses of the fungi. The study demonstrated that ionones significantly inhibited mycelial growth in both <em>Foln</em> and <em>Fo47</em> strains. Our results also showed the differences in apocarotenoid's effect on studied strains in regard of sporulation, FUB genes cluster activity and fusaric acid (FA) production.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101591"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of fungal volatile organic compounds for enhancing plant growth and controlling plant pathogens","authors":"Elsherbiny A. Elsherbiny ,&nbsp;Sabrien A. Omar ,&nbsp;Joan W. Bennett","doi":"10.1016/j.funbio.2025.101606","DOIUrl":"10.1016/j.funbio.2025.101606","url":null,"abstract":"<div><div>Volatile organic compounds (VOCs) play important and diverse roles in the environment. In complex ecological communities, they mediate inter-organismal communication. The physical properties of VOCs enable them to diffuse through the air and impact above-ground plant parts, as well as affect underground plant parts in gas-filled pores in the soil. Many microbial VOCs stimulate plant growth and/or inhibit the growth of plant pathogens. Because VOCs do not rely on direct contact between the biocontrol organism and the pathogen, they can work at a distance. However, VOCs easily dissipate into the atmosphere, and the physiological effects of VOCs are highly concentration-dependent, which pose challenges for controlled applications. Currently, their greatest utility is for storage pathogens and greenhouse crops. This review summarizes important findings about fungal VOCs from an agricultural perspective, giving examples of plant growth promotion and the inhibition of plant pathogens while also highlighting the challenges that accompany attempts to harness VOCs activity for agricultural productivity.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101606"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycogenic synthesis of silver nanoparticles using endophytic fungi and their characterization, biological activities, including in-silico studies with special reference to Fusarium wilt of tomato","authors":"Susmita Jana ,&nbsp;Dona Das ,&nbsp;Sankar Bhattacharyya ,&nbsp;Subrata Raha","doi":"10.1016/j.funbio.2025.101610","DOIUrl":"10.1016/j.funbio.2025.101610","url":null,"abstract":"<div><div>Nanoparticle research is currently a topic of significant scientific interest, due to its vast array of application in biological field. An effort was made to produce silver nanoparticles (AgNPs) from two endophytic fungi; <em>Fusarium oxysporum</em> (FoAgNPs) and <em>Fusarium proliferatum</em> (FpAgNPs), which were isolated from a Pteridophyte <em>Pyrrosia lanceolata</em> (L.) Farw. The AgNPs were characterised using UV–Vis spectroscopy, exhibiting sizes ranging from 3 nm to 27 nm and displaying a polycrystalline nature as determined by scanning and transmission microscopy, along with SAED pattern analysis. Additionally, we identify phenolic groups at 1067.12 cm⁻¹ as the capping agent that facilitates the reduction of silver ions and stabilizes the nanoparticles evaluated via FTIR. The <em>in vitro</em> antibacterial potency AgNPs had the maximum activity against <em>Escherichia fergusonii</em>, followed by <em>Proteus mirabilis</em>; for both organisms, the minimum inhibitory concentration (MIC) value was 10 μg/ml. AgNPs also demonstrated strong antifungal activity against various plant pathogens, MIC was 15 μg/ml. Additionally, SEM analysis revealed that AgNPs caused pathogen hypha shrinkage and deformation, indicating structural deterioration in cellular and organelle structures due to ROS production. Further, the antifungal efficacy of manufactured AgNPs was investigated against <em>F. oxysporum</em> f.sp. <em>lycopersici</em> in <em>Solanum lycopersicum</em>, a plant pathogen affecting tomato growth and yield, and nano-formulation (150 ppm) completely prevented infection in the greenhouse settings. Biogenic AgNPs at 40 ppm enhanced root-shoot length in <em>Vigna radiata</em> seeds compared to untreated seeds, suggesting phyto-stimulatory action. The cytotoxicity assessment indicated that the synthesized AgNPs are safe for a variety of bio-applications. Furthermore, we chose NADPH Oxidase (<em>F. oxysporum</em> f.sp. <em>lycopersici</em>) as the protein to perform the molecular docking and results indicates that the active site of the selected protein serves as a critical region for inhibiting disease propagation. The study findings enhanced our knowledge of the antifungal properties and mechanisms of AgNPs, providing a novel perspective on utilising this antifungal alternative for the treatment of plant diseases.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101610"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological-degradation, lignin performance and physical-chemical characteristics of historical wood in an ancient tomb","authors":"Akbar Mastouri ,&nbsp;Davood Efhamisisi ,&nbsp;Martin Lexa ,&nbsp;Reza Oladi ,&nbsp;Alireza Gholinejad-Pirbazari ,&nbsp;Hossein Torabi ,&nbsp;Aleš Zeidler ,&nbsp;Mariaenrica Frigione","doi":"10.1016/j.funbio.2025.101588","DOIUrl":"10.1016/j.funbio.2025.101588","url":null,"abstract":"<div><div>Biodeterioration assessments of archaeological woods, also comparisons with recent wood, provides an effective strategy for its conservation in cultural-heritage. This research aimed to analyze some wooden structures of an ancient tomb, situated in West-Azerbaijan province of Iran adjacent to Lake-Urmia. The anatomical characteristics of historical wood (HW) was investigated to identify species and trace xylem micro-morphological variations in detail. Chemical-changes of deteriorated wood were examined using X-ray diffraction (XRD) and ATR-FTIR spectroscopy, and its wood–water interactions were compared with the recent new-wood. Macro/micro-morphological and mycological assessment of historical poplar wood (<em>Populus alba</em>) confirmed signs of soft-rot fungi, and recent attacks by wood–boring (Xylophagous) insects probably due to local climate-change. Type-I microcavities and advanced soft-rot decay caused cell-walls depletion or deformation, which was visually observed in HW by discoloration and transverse-cracks. Based on Optical- and SEM-microscopy, lignin preservation was observed in the middle-lamella and especially the vessels rich in guaiacyl-lignin (G-type) units. However, the fiber walls were susceptible to fungal degradation due to syringyl-lignin (S-type). Histochemical changes increased the porosity, hygroscopicity, and especially water-absorption (1h–720h) of HW compared to the corresponding new-wood, introducing the severity of damage and treatability criteria for conservationists. Lignin increment, loss of cellulose-crystallinity in XRD (70.5 to 59.9 %) and FTIR ratios (1280/1200, 1317/1336), and also preferential-degradation of polysaccharides were confirmed through chemical analyses of soft-rot decayed wood. These findings, in addition to helping monitor potential risks of wooden artifacts, especially non-durable species, facilitate preventive and protective management of archaeological-heritage in both wet and dry environments.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 4","pages":"Article 101588"},"PeriodicalIF":2.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143867827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Talaromyces purpureogenus-mediated mycosynthesis of aluminium oxide nanoparticles and characterization of the physicochemical properties at different calcination temperatures","authors":"Muhammad Salahudin Kheirel Anuar ,&nbsp;Che Azurahanim Che Abdullah ,&nbsp;Muhammad Farhan Nazarudin ,&nbsp;Shamsu Ibrahim Ishaq ,&nbsp;Mohammad Noor Amal Azmai ,&nbsp;Annas Salleh ,&nbsp;Ina Salwany Md Yasin ,&nbsp;Mohd Termizi Yusof","doi":"10.1016/j.funbio.2025.101570","DOIUrl":"10.1016/j.funbio.2025.101570","url":null,"abstract":"<div><div>The study reports an environmentally friendly and simple green technique for the synthesis of aluminium oxide nanoparticles (Al₂O₃-NPs). The green synthesis of Al₂O₃-NPs using natural products has garnered significant interest due to their non-toxic, cost-effective, convenient, and eco-friendly nature. However, there is limited literature on the green synthesis of Al₂O₃-NPs using fungal materials and the influence of calcination temperatures on the physicochemical characteristics of mycogenic nanoparticles (NPs). To date, no studies have explored the influence of calcination temperatures on the physicochemical characteristics of mycogenic Al₂O₃-NPs. This study aimed to compare and characterize the mycogenic synthesis of Al₂O₃-NPs and investigate how three different calcination temperatures influence the structural properties of the NPs. In this study, Al₂O₃-NPs were synthesized using the <em>Talaromyces purpureogenus</em> isolate SD7 through extracellular production. To study the effects of calcination temperatures on the physicochemical properties of the NPs, the mycogenic Al₂O₃-NPs were calcined at temperatures ranging from 550 °C to 750 °C based on thermogravimetric analysis. The samples were further characterized using various spectroscopic and imaging techniques. The analysis of ultraviolet–visible (UV–Vis) spectrophotometer, X-ray diffraction (XRD), and high-resolution transmission electron microscopy (HR-TEM) demonstrated that particle size escalated with elevated calcination temperatures. Characterization techniques using field emission scanning electron microscopy (FESEM), HR-TEM, and XRD confirmed that the Al₂O₃-NPs exhibited both rod-like, spherical structures with irregular sizes, and showed the presence of nanocrystalline γ-Al₂O₃. However, only the sample calcined at 550 °C remains within the nanoscale range, whereas samples treated at higher temperatures show considerable grain growth, exceeding the nanometer scale. This study showcases the potential of mycosynthesized Al₂O₃-NPs using fungal material as an eco-friendly alternative. It also emphasizes the significant influence of calcination temperatures on the structural and physicochemical properties of mycogenic Al₂O₃-NPs, underscoring their suitability for a wide range of applications.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 4","pages":"Article 101570"},"PeriodicalIF":2.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leading developments in basic research on aspergillosis and mucormycosis","authors":"Argha Sarkar ,&nbsp;Drauzio E.N. Rangel ,&nbsp;Nir Osherov","doi":"10.1016/j.funbio.2025.101574","DOIUrl":"10.1016/j.funbio.2025.101574","url":null,"abstract":"<div><div>This editorial presents an overview of notable contributions in basic research on aspergillosis and mucormycosis published between 2022 and 2024. Basic research in aspergillosis saw major advances in the field of genomics brought about by inexpensive whole-genome sequencing of hundreds of isolates. This has deepened our understanding of <em>Aspergillus fumigatus</em> population structure, gene diversity, and the evolution of azole antifungal resistance. Basic research in mucormycosis saw interesting developments in our understanding of the interactions between <em>Rhizopus microsporus</em> and endosymbiotic bacteria that protect it against predatory soil amoeba and increase its virulence in models of infection.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 4","pages":"Article 101574"},"PeriodicalIF":2.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic evaluation of fluoride quantification methods identifies an appropriate measurement for studying fungal defluorination of per- and Polyfluoroalkyl substances","authors":"Charles Ayers,&nbsp;Jiwei Zhang","doi":"10.1016/j.funbio.2025.101586","DOIUrl":"10.1016/j.funbio.2025.101586","url":null,"abstract":"<div><div>The large-scale manufacturing and disposal of Per- and Polyfluoroalkyl substances (PFASs) are causing global concerns, and investigations are needed to understand their ecological impacts. Saprotrophic fungi are dominant nutrient recyclers in ecosystems, while their roles in transforming PFAS remain largely untapped. Particularly, there is a lack of appropriate means to rapidly measure fluoride anions released by fungal cultures to understand their defluorination (deF) capacities and mechanisms. In this research, we systematically evaluated three prevalent means for quantifying free fluoride anions under cultural conditions of wood decay fungal species. Two spectrophotometric measurings, including one based on the fluoride-catalyzed kinetochromic reaction of Xylenol Orange-Zirconium (XO-Zr⁴⁺) and another one based on Alizarin-Lanthanum-Fluoride (Al–La³⁺-F^-) ternary complexone reaction, were successfully adapted to a high-throughput micro-well setup for detecting micromolar-level fluoride, but essential fungal media components pose significant interference to both assays. The third method, relying on the potentiometer and fluoride ion-selective electrode (F-ISE), demonstrated a strong capacity for anti-interferences of fungal nutrients, metabolites, and PFAS, leaving it a most appropriate method to study fungal deF. With F-ISE, we further demonstrated a deF test using a model PFAS, 4,4,4-trifluoro-3-(trifluoromethyl) crotonic acid, and a wood rot fungus <em>Trametes versicolor</em>. Together, our research identifies a reliable fluoride quantification method that can allow assessing fungal cultures for their PFAS-defluorinating phenotypes.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 4","pages":"Article 101586"},"PeriodicalIF":2.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}