Isolation and characterization of native fungal strains for the bioremediation of BTEX pollutants

IF 2.9 3区生物学 Q2 MYCOLOGY

Fungal biology Pub Date : 2025-02-01 DOI:10.1016/j.funbio.2024.101534

Reyhaneh Shekari , Parisa Mohammadi , Gholamreza Zarrini

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引用次数: 0

Abstract

Benzene, toluene, ethylbenzene, and xylene (BTEX) exposure is known to be carcinogenic and neurotoxic chemicals to humans. This study investigates the potential of fungal native strains for the bioremediation of BTEX compounds. Fungal isolates were obtained from BTEX-enriched soil, and their ability to degrade these pollutants was evaluated. Gas Chromatography-Mass Spectrometry (GC–MS) method has been used to confirm the biodegradation of BTEX. Eight fungal strains were isolated from genera such as Talaromyces, Aspergillus, and Lichtheimia. Among them, Talaromyces pinophilus RBF1 and Aspergillus elsenburgensis RBF6 were very efficient strains for BTEX biodegradation over 98 % removal of BTEX at a concentration of 1 %. The strains also grow very well at a higher concentration of 2 % BTEX. In contrast, Lichtheimia ramosa RBF8 and Aspergillus nidulans RBF2 exhibited increased benzene concentration, which probably occurred due to biological transformation of other compounds into benzene. Despite the high bioremediation potential of some isolated fungi, further assessment of fungal degradation is necessary to develop bioremediation strategies.

Abstract Image

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BTEX污染物生物修复天然真菌菌株的分离与鉴定。

已知接触苯、甲苯、乙苯和二甲苯（BTEX）对人类具有致癌和神经毒性化学物质。本研究探讨了真菌原生菌株对BTEX化合物进行生物修复的潜力。从富含btex的土壤中分离得到真菌，并对其降解这些污染物的能力进行了评价。采用气相色谱-质谱（GC-MS）法对BTEX的生物降解进行了确证。从Talaromyces属、Aspergillus属和Lichtheimia属中分离到8株真菌。其中，嗜松Talaromyces pinophilus RBF1和elsenburgenaspergillus RBF6对BTEX的生物降解效率最高，在浓度为1%的条件下，BTEX的去除率可达98%。菌株在2% BTEX浓度下也生长良好。相比之下，苔藓曲霉（Lichtheimia ramosa） RBF8和灰曲霉（Aspergillus nidulans） RBF2的苯浓度升高，这可能是由于其他化合物转化为苯所致。尽管一些分离的真菌具有很高的生物修复潜力，但进一步评估真菌的降解是制定生物修复策略的必要条件。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fungal biology MYCOLOGY-

CiteScore

5.80

自引率

4.00%

发文量

审稿时长

49 days

期刊介绍： Fungal Biology publishes original contributions in all fields of basic and applied research involving fungi and fungus-like organisms (including oomycetes and slime moulds). Areas of investigation include biodeterioration, biotechnology, cell and developmental biology, ecology, evolution, genetics, geomycology, medical mycology, mutualistic interactions (including lichens and mycorrhizas), physiology, plant pathology, secondary metabolites, and taxonomy and systematics. Submissions on experimental methods are also welcomed. Priority is given to contributions likely to be of interest to a wide international audience.