Fungal biology最新文献_第2页

The role of Pep4 protease in Cryptococcus neoformans cell survival and virulence factors Pep4蛋白酶在新生隐球菌细胞存活及毒力因子中的作用

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-06-03 DOI: 10.1016/j.funbio.2025.101611

Gabrielle Felizardo , Adrián Adolfo Álvarez Padilla , Amanda Teixeira de Melo , Ricardo Ferreira Lima , Grasielle Pereira Jannuzzi , Kevin Felipe Cruz Martho , Sandro Rogério de Almeida , Karen Spadari Ferreira , Renata Castiglioni Pascon , Marcelo Afonso Vallim

{"title":"The role of Pep4 protease in Cryptococcus neoformans cell survival and virulence factors","authors":"Gabrielle Felizardo , Adrián Adolfo Álvarez Padilla , Amanda Teixeira de Melo , Ricardo Ferreira Lima , Grasielle Pereira Jannuzzi , Kevin Felipe Cruz Martho , Sandro Rogério de Almeida , Karen Spadari Ferreira , Renata Castiglioni Pascon , Marcelo Afonso Vallim","doi":"10.1016/j.funbio.2025.101611","DOIUrl":"10.1016/j.funbio.2025.101611","url":null,"abstract":"<div><div>Cryptococcosis is a systemic mycosis caused by <em>Cryptococcus neoformans</em> with significant clinical importance, mainly affecting immunodeficient patients. The treatment options are limited to a few drugs, and resistance to them has been reported. Therefore, research is essential to broaden knowledge regarding the biology of this yeast, aiming to identify traits that could serve as new targets for antifungal drugs. This study aims to expand the current understanding of the autophagy process in this pathogenic yeast. Autophagy is a conserved intracellular degradation and recycling process among eukaryotes, indispensable in cellular homeostasis. In <em>Saccharomyces cerevisiae</em>, the <em>PEP4</em> gene encodes a protease required during the final stages of autophagy, playing a role in the maturation and activation of vacuolar hydrolases, which contributes to cell survival under conditions of nutritional deprivation and stress. However, <em>PEP4</em> has never been studied in <em>C. neoformans</em>. Thus, we evaluated the impact of <em>PEP4</em> deletion on the expression of virulence factors and the cell response to multiple stress conditions. Our results demonstrated that the <em>pep4</em>Δ mutant exhibited attenuated virulence in <em>Galleria mellonella</em> and a decreased fungal burden in macrophages. Notably, we observed the accumulation of autophagic bodies in the <em>pep4</em>Δ strain under nutrient starvation, suggesting a defect in the final steps of autophagic degradation. These findings suggest that the Pep4 protein of <em>C. neoformans</em> plays a crucial role in vacuolar function and the adaptation and survival of yeast cells under stressful conditions, as well as in the host–pathogen interaction.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101611"},"PeriodicalIF":2.9,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome analysis reveals the molecular mechanisms of the 8-day infection cycle of Ecytonucleospora hepatopenaei in Litopenaeus vannamei and the host's molecular responses 转录组分析揭示了凡纳滨对虾（Litopenaeus vannamei） 8天感染周期的分子机制和宿主的分子反应

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-06-03 DOI: 10.1016/j.funbio.2025.101613

Yan Rong , Yi Qiao , Tingyue Liu , Xiaohui Cao , Ge Jiang , Jie Cheng , Huiwen Jiang , Xianping Fan , Hui Shen

{"title":"Transcriptome analysis reveals the molecular mechanisms of the 8-day infection cycle of Ecytonucleospora hepatopenaei in Litopenaeus vannamei and the host's molecular responses","authors":"Yan Rong , Yi Qiao , Tingyue Liu , Xiaohui Cao , Ge Jiang , Jie Cheng , Huiwen Jiang , Xianping Fan , Hui Shen","doi":"10.1016/j.funbio.2025.101613","DOIUrl":"10.1016/j.funbio.2025.101613","url":null,"abstract":"<div><div><em>Ecytonucleospora hepatopenaei</em> (EHP), an emerging microsporidian, has caused significant economic losses to the Asian aquaculture industry. This study investigates the infection dynamics of EHP through deep transcriptomic sequencing of infected shrimp samples at different time points. The analysis revealed distinct periodic changes in metabolism, immune response, and DNA repair during EHP infection. Shrimp rapidly mobilize energy metabolism and activate immune pathways in the early stage (day 2), followed by immune suppression and metabolic regulation in the mid-stage (days 4–6), and enhanced immune responses by day 8. Trend analysis supports an 8-day infection cycle, with periodic changes in gene expression profiles, especially in energy metabolism, protein synthesis and degradation, and immune responses. Host gene expression displays a phased response aligning with EHP proliferation and its life cycle. EHP's gene expression also shows periodicity, with upregulation of proliferation-associated genes on days 4 and 10. Weighted Gene Co-expression Network Analysis (WGCNA) identified key gene modules related to metabolic adaptation and immune suppression, indicating EHP's adaptation to the host environment. The study reveals a cyclical pattern of interaction between EHP and its host, providing insights into EHP's pathogenic mechanisms.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101613"},"PeriodicalIF":2.9,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of entomopathogenic fungus Cordyceps cicadae isolated from soil using common cutworm Spodoptera litura (Lepidoptera: Noctuidae) as bait and its high virulence comparable to generalist Metarhizium anisopliae complex 以普通夜蛾Spodoptera litura（鳞翅目：夜蛾科）为饵从土壤中分离出昆虫病原真菌蝉虫草及其毒力可与金龟子绿僵菌复合体相媲美的鉴定

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-06-02 DOI: 10.1016/j.funbio.2025.101612

Duong Danh Thanh , Oumi Nishi , Naoya Wasano , Chisa Yasunaga-Aoki

{"title":"Identification of entomopathogenic fungus Cordyceps cicadae isolated from soil using common cutworm Spodoptera litura (Lepidoptera: Noctuidae) as bait and its high virulence comparable to generalist Metarhizium anisopliae complex","authors":"Duong Danh Thanh , Oumi Nishi , Naoya Wasano , Chisa Yasunaga-Aoki","doi":"10.1016/j.funbio.2025.101612","DOIUrl":"10.1016/j.funbio.2025.101612","url":null,"abstract":"<div><div>The common cutworm (<em>Spodoptera litura,</em> Lepidoptera: Noctuidae) is one of the most widespread pest insects affecting various crops in Asian countries. To screen promising biological control agents for this pest, we isolated 49 potential entomopathogenic fungal isolates from 25 soil samples using <em>S. litura</em> and four additional insect species as baits. The results revealed a high occurrence (24 %) of <em>Cordyceps cicadae</em> in soil through the bait method with all five insect baits, following the <em>Metarhizium anisopliae</em> complex (52 %), despite <em>Cordyceps cicadae</em> generally being known as a specialist entomopathogenic fungus of cicada nymphs. Molecular phylogenetic analysis demonstrated that <em>Cordyceps cicadae</em> isolates from soil and natural cicada nymphs clustered together at a terminal node with previously reported <em>Cordyceps cicadae</em> from cicada nymphs and <em>C. lepidopterorum</em> from lepidopteran larvae. Virulence assays against last-instar larvae of <em>S. litura</em> revealed significant variability in virulence among <em>C. cicadae</em> strains derived from soil and cicada nymphs. Among these, <em>C. cicadae</em> S17, isolated from soil using <em>S. litura</em> as bait, exhibited virulence comparable to the most virulent strains of the <em>M. anisopliae</em> complex and was also virulent against third-instar larvae and pupae. Our findings indicate that <em>C. cicadae</em> exhibit a broader host range than previously recognized, with potential applications in biological control for larvae and pupae of <em>S. litura</em>.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101612"},"PeriodicalIF":2.9,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sterol aminoacylation regulates conidiation and sclerotia formation in Aspergillus oryzae 甾醇氨基酰化调节米曲霉的分生和菌核形成

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-06-01 DOI: 10.1016/j.funbio.2025.101609

Daisuke Yokokawa , Shunsuke Tatematsu , Makoto Imai , Yoshihiro Takara , Yusuke Saga , Frédéric Fischer , Hubert D. Becker , Harushi Nakajima , Tetsuo Kushiro

{"title":"Sterol aminoacylation regulates conidiation and sclerotia formation in Aspergillus oryzae","authors":"Daisuke Yokokawa , Shunsuke Tatematsu , Makoto Imai , Yoshihiro Takara , Yusuke Saga , Frédéric Fischer , Hubert D. Becker , Harushi Nakajima , Tetsuo Kushiro","doi":"10.1016/j.funbio.2025.101609","DOIUrl":"10.1016/j.funbio.2025.101609","url":null,"abstract":"<div><div>Ergosterol is a major fungal membrane component that contributes to membrane functions. We recently discovered novel ergosterol derivatives conjugated with amino acids, namely two aminoacylated ergosterols (Erg-aa), 1-ergosteryl-L-aspartate (Erg-Asp) and 1-ergosteryl-glycine (Erg-Gly), and the synthase involved (ErdS and ErgS, respectively). These enzymes are widely conserved in fungi belonging to the Dikarya subkingdom. However, the physiological role of Erg-aa remains unknown. Here, we report that Erg-aa are involved in conidiation and sclerotia formation in <em>Aspergillus oryzae</em>. The deletion of <em>AoerdS</em> or <em>AoergS</em> led to the reduction of conidia, accompanied by the downregulation of conidiation-regulating genes. In contrast, these deletants formed sclerotia considerably. <em>AoerdS</em> inducible strains also decreased conidia and promoted sclerotia formation under <em>AoerdS</em> suppressing condition, whereas the opposite morphology was observed under <em>AoerdS</em> inductive condition. We also investigated the effects of synthetic Erg-aa on fungal growth. Externally added Erg-Asp slightly promoted conidiation but did not affect sclerotia formation. On the other hand, Erg-Gly promoted the elongation of aerial hyphae and reduced both conidia and sclerotia. Finally, the deletion of <em>AoerdS</em> or <em>AoergS</em> altered susceptibility to ergosterol associating agents, indicating that ergosterol aminoacylation may change the physicochemical properties of ergosterol. Our findings expand the biological role of sterol modification in fungi.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101609"},"PeriodicalIF":2.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144229903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat stress priming enhanced conidial germination, insect virulence and metabolic adaptation in Beauveria bassiana 热胁迫增强球孢白僵菌孢子萌发、昆虫毒力和代谢适应

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-31 DOI: 10.1016/j.funbio.2025.101607

Somsiri Udompaisarn , Jiraporn Jirakkakul , Thanawat Duangfoo , Wachiraporn Toopaang , Alongkorn Amnuaykanjanasin

{"title":"Heat stress priming enhanced conidial germination, insect virulence and metabolic adaptation in Beauveria bassiana","authors":"Somsiri Udompaisarn , Jiraporn Jirakkakul , Thanawat Duangfoo , Wachiraporn Toopaang , Alongkorn Amnuaykanjanasin","doi":"10.1016/j.funbio.2025.101607","DOIUrl":"10.1016/j.funbio.2025.101607","url":null,"abstract":"<div><div><em>Beauveria bassiana</em> strains are commercially available as mycoinsecticides and are widely used for insect control. However, its effectiveness is often hindered by vulnerability to environmental stressors such as UV radiation, drought, and heat. Exposure to moderate heat, which is considered a triggering stimulus, partially inhibits the germination of conidia. This study investigated the impact of heat stress and priming on conidial germination, radial growth, sporulation, virulence to insects, and metabolic profiling in <em>B</em>. <em>bassiana</em> BCC 2660. The conidial germination significantly suppressed and declined from 82 to 34 % at 45 °C after 180 min. Here, we introduced a priming state to enhance the fungus's tolerance to the triggering stimulus. Comparative analysis revealed that conidia subjected to heat-induced priming at 35 °C for 40 min exhibited a significant increase in germination from 32 to 65 %, compared to non-primed conidia. The primed conidia also demonstrated a reduction in the LT<sub>50</sub> by 10 h in comparison to their non-primed counterparts. While priming improved germination, it did not significantly affect radial growth or sporulation rates under heat stress. We further explored the memory time of priming, finding that the fungus retained the priming state for up to 4 h. To elucidate the molecular mechanisms underlying the heat stress response, we identified candidate metabolites using LC-MS. Metabolomic analysis identified 34 metabolites, with significant alterations in metabolic pathways related to spermidine biosynthesis, coenzyme A synthesis, fungal wall development and various cellular process among primed conidia. These results underscored the complex metabolic adjustments in <em>B. bassiana</em> conidia, highlighting the potential of priming strategies to enhance fungal tolerance to environmental stresses and improve biocontrol efficacy in agricultural applications.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101607"},"PeriodicalIF":2.9,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144229902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering the morphological and phylogenetic diversity of mushrooms in pecan orchards in the Southwestern United States 揭示美国西南部山核桃果园中蘑菇的形态和系统发育多样性

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-30 DOI: 10.1016/j.funbio.2025.101608

McKenzie L. Stock , Richard J. Heerema , Jennifer J. Randall , Adriana L. Romero-Olivares , Samuel A. Belteton , Ciro Velasco-Cruz , Nicole Pietrasiak

{"title":"Uncovering the morphological and phylogenetic diversity of mushrooms in pecan orchards in the Southwestern United States","authors":"McKenzie L. Stock , Richard J. Heerema , Jennifer J. Randall , Adriana L. Romero-Olivares , Samuel A. Belteton , Ciro Velasco-Cruz , Nicole Pietrasiak","doi":"10.1016/j.funbio.2025.101608","DOIUrl":"10.1016/j.funbio.2025.101608","url":null,"abstract":"<div><div>Symbiotic relationships between pecan tree roots and mycorrhizal fungi may enhance drought and salt tolerance, nutrient absorption, and disease resistance in pecan (<em>Carya illinoinensis</em> (Wangenh.) K. Koch) orchards of the arid Southwestern United States. Saprotrophic fungi contribute to orchard ecosystem health by breaking down organic matter and enriching soil quality. However, we currently have limited, mostly anecdotal knowledge on mushroom-forming fungi in Southwestern pecan orchards, and studies in other regions primarily focus on ectomycorrhizal fungi. This study provides a novel mycological survey of mushroom-forming fungi (both ectomycorrhizal and saprotrophic) in pecan orchards across New Mexico, Arizona, West Texas, and California. In collaboration with pecan producers, sporocarps from 31 different fungal taxa were collected for morphological, phylogenetic, and ecological analyses. UNITE and NCBI databases aided in taxon identification to construct a maximum-likelihood phylogenetic tree. Common ectomycorrhizal taxa included <em>Scleroderma</em> species<em>, Pisolithus tinctorius,</em> and <em>Tuber lyonii</em><em>.</em>Saprotrophs included <em>Chlorophyllum molybdites</em><em>,</em> the regionally-unique <em>Agaricus deserticola</em>, and others. Logistic regression revealed bare soil and tree row location as significant predictors for mycorrhizal fungi presence. This study offers an inaugural characterization of mushroom-forming fungal communities in Southwestern pecan orchards and highlights their potential roles in enhancing orchard ecosystem health and resilience.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101608"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential profiling of viable fungi in the gut of Penaeus vannamei cultured in brackishwater ponds with high protein, low protein and azolla-based diets 在高蛋白、低蛋白和以氮唑为基础饲料的咸淡水中养殖的凡纳滨对虾肠道内活菌的差异分析

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-29 DOI: 10.1016/j.funbio.2025.101595

N.S. Sudheer , I.F. Biju , L. Priyadarshini , D. De , T.K. Ghoshal , Babita Mandal , S. Das

{"title":"Differential profiling of viable fungi in the gut of Penaeus vannamei cultured in brackishwater ponds with high protein, low protein and azolla-based diets","authors":"N.S. Sudheer , I.F. Biju , L. Priyadarshini , D. De , T.K. Ghoshal , Babita Mandal , S. Das","doi":"10.1016/j.funbio.2025.101595","DOIUrl":"10.1016/j.funbio.2025.101595","url":null,"abstract":"<div><div>An understanding of the gut microflora of an organism provides vital information that could be used in developing strategies for health management. In this view, a differential profiling of fungi in the gut of <em>Penaeus vannamei</em> fed on high protein, low protein and azolla-based diets are carried out. Shrimp samples were collected from brackish water ponds maintained on various dietary changes and fungus species were isolated and identified. A total of 43 fungi isolates belonging to 29 species, 2 phyla, 8 classes, 11 orders, 22 families and 23 genera ware identified. Among the identified species 74.41 % are represented by phylum Ascomycota and 25.60 % of the species are represented by Basidiomycota. <em>Sakaguchia oryzae</em> (9 %) and <em>Parasarocladium breve</em> (9 %) were the dominant species. The data indicate that higher fungal diversity was present in low protein administered groups followed by high protein feed with a slight difference. The lowest fungal diversity was in the azolla-based diet administered group. <em>P. breve</em> and <em>Penicillium osxalicum</em> were abundant in the high protein feed administered groups. <em>S. oryzae</em> and <em>Acremonium brachypenium</em> ware abundant in low protein and azolla-based feed groups, respectively. The genera Acremonium (4.3 %) was found common in all tested diet administered groups. The results indicated that dietary changes may influence the fungal diversity in the gut of <em>P. vannamei</em>. Our study provides valuable information on the gut mycobiota of <em>P. vannamei</em> cultured on dietary changes through employing modern data analysis and visualization tools, which is essential for developing an effective management strategy to improve shrimp culture.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101595"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of fungal volatile organic compounds for enhancing plant growth and controlling plant pathogens 真菌挥发性有机化合物在促进植物生长和控制植物病原体中的应用

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-28 DOI: 10.1016/j.funbio.2025.101606

Elsherbiny A. Elsherbiny , Sabrien A. Omar , Joan W. Bennett

{"title":"Application of fungal volatile organic compounds for enhancing plant growth and controlling plant pathogens","authors":"Elsherbiny A. Elsherbiny , Sabrien A. Omar , Joan W. Bennett","doi":"10.1016/j.funbio.2025.101606","DOIUrl":"10.1016/j.funbio.2025.101606","url":null,"abstract":"<div><div>Volatile organic compounds (VOCs) play important and diverse roles in the environment. In complex ecological communities, they mediate inter-organismal communication. The physical properties of VOCs enable them to diffuse through the air and impact above-ground plant parts, as well as affect underground plant parts in gas-filled pores in the soil. Many microbial VOCs stimulate plant growth and/or inhibit the growth of plant pathogens. Because VOCs do not rely on direct contact between the biocontrol organism and the pathogen, they can work at a distance. However, VOCs easily dissipate into the atmosphere, and the physiological effects of VOCs are highly concentration-dependent, which pose challenges for controlled applications. Currently, their greatest utility is for storage pathogens and greenhouse crops. This review summarizes important findings about fungal VOCs from an agricultural perspective, giving examples of plant growth promotion and the inhibition of plant pathogens while also highlighting the challenges that accompany attempts to harness VOCs activity for agricultural productivity.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101606"},"PeriodicalIF":2.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overview of scientific productivity in the field of fungal stress research 真菌胁迫研究领域科学生产力综述

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-24 DOI: 10.1016/j.funbio.2025.101598

Alene Alder-Rangel , Drauzio E.N. Rangel

{"title":"Overview of scientific productivity in the field of fungal stress research","authors":"Alene Alder-Rangel , Drauzio E.N. Rangel","doi":"10.1016/j.funbio.2025.101598","DOIUrl":"10.1016/j.funbio.2025.101598","url":null,"abstract":"<div><div>The study of fungal stress (FS) responses contributes to a deeper understanding of this important group of organisms. This bibliometric study explores what research has been conducted on how agricultural, environmental, and industrial important fungi respond to changes or stress in their environment. The Web of Science was employed to find publications related to FS tolerance, and after sorting, 3126 articles were analyzed. The major fungus/fungi studied in each article were noted and sorted into categories of Environmental, Industrial, or Agricultural FS. The publications were then assigned into these categories or General, for articles covering many fungi. Agricultural Fungi were further divided into Plant-pathogenic, Insect-pathogenic, Antagonist, Postharvest decay, and Nematode-trapping niches. Seventy years of research into FS were found, with the majority of publications after 1990, peaking in 2022. Almost half of the FS publications were about Agriculture, and plant pathogens were the most common niche studied. The most studied fungi were <em>Beauveria bassiana, Saccharomyces cerevisiae</em>, and <em>Aspergillus nidulans</em>. Studies on FS were conducted in 87 countries by at least 9766 researchers. Most of the top institutions and researchers in FS publications are Chinese.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101598"},"PeriodicalIF":2.9,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of phenotype and secondary metabolic silencing gene clusters in Aspergillus sydowii by velvet transcription factors 丝绒转录因子对西多曲霉表型和次生代谢沉默基因簇的调控

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-23 DOI: 10.1016/j.funbio.2025.101605

Jie Zeng , Yu Cao , Yihan Guo , Dongyou Xiang , Jiale Wang , Qingrui Xu , Xiaoqiang Lang , Hui Xu , Yi Cao

{"title":"Regulation of phenotype and secondary metabolic silencing gene clusters in Aspergillus sydowii by velvet transcription factors","authors":"Jie Zeng , Yu Cao , Yihan Guo , Dongyou Xiang , Jiale Wang , Qingrui Xu , Xiaoqiang Lang , Hui Xu , Yi Cao","doi":"10.1016/j.funbio.2025.101605","DOIUrl":"10.1016/j.funbio.2025.101605","url":null,"abstract":"<div><div>The silencing of biosynthetic gene clusters (BGCs) is a widespread phenomenon in fungal secondary metabolism. Traditional environmental induction strategies often fail to effectively activate these silent BGCs, whereas global transcription factor regulation has emerged as a powerful strategy for their activation. Therefore, this study focuses on Velvet family transcription factors VosA and VelB, investigating their functions in <em>Aspergillus sydowii</em> H-1 and their regulatory role in activating silent gene clusters. Phenotypic analyses revealed that <em>AsvelB</em> influence conidiation, colony morphology. Disruption or overexpression of <em>AsvosA</em> or <em>AsvelB</em> resulted in impaired cell wall integrity (CWI). Furthermore, the knockout strains Δ<em>AsvelB</em> and Δ<em>AsvosA</em> exhibited elevated intracellular reactive oxygen species (ROS) levels. These findings highlight their roles in maintaining cellular homeostasis and fungal physiology. Semi-quantitative PCR analysis of 19 type I polyketide synthase (PKS) genes revealed that <em>AsvosA</em> and <em>AsvelB</em> exert global regulatory effects on secondary metabolism in H-1. <em>EVM0012129.1</em> was identified as a candidate PKS gene highly associated with purple pigment biosynthesis through semi-quantitative PCR and qPCR analyses. Overexpression of <em>AsvosA</em> enhanced purple pigment production specifically in conidia and upregulated <em>EVM0012129.1</em>, further supporting its critical role in pigment synthesis. These findings provide new insights into the regulatory network of <em>AsvosA</em> and <em>AsvelB</em>, highlighting their potential applications in fungal biology and metabolic engineering.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101605"},"PeriodicalIF":2.9,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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