Fungal biology最新文献_第2页

Heat stress priming enhanced conidial germination, insect virulence and metabolic adaptation in Beauveria bassiana 热胁迫增强球孢白僵菌孢子萌发、昆虫毒力和代谢适应

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-31 DOI: 10.1016/j.funbio.2025.101607

Somsiri Udompaisarn , Jiraporn Jirakkakul , Thanawat Duangfoo , Wachiraporn Toopaang , Alongkorn Amnuaykanjanasin

{"title":"Heat stress priming enhanced conidial germination, insect virulence and metabolic adaptation in Beauveria bassiana","authors":"Somsiri Udompaisarn , Jiraporn Jirakkakul , Thanawat Duangfoo , Wachiraporn Toopaang , Alongkorn Amnuaykanjanasin","doi":"10.1016/j.funbio.2025.101607","DOIUrl":"10.1016/j.funbio.2025.101607","url":null,"abstract":"<div><div><em>Beauveria bassiana</em> strains are commercially available as mycoinsecticides and are widely used for insect control. However, its effectiveness is often hindered by vulnerability to environmental stressors such as UV radiation, drought, and heat. Exposure to moderate heat, which is considered a triggering stimulus, partially inhibits the germination of conidia. This study investigated the impact of heat stress and priming on conidial germination, radial growth, sporulation, virulence to insects, and metabolic profiling in <em>B</em>. <em>bassiana</em> BCC 2660. The conidial germination significantly suppressed and declined from 82 to 34 % at 45 °C after 180 min. Here, we introduced a priming state to enhance the fungus's tolerance to the triggering stimulus. Comparative analysis revealed that conidia subjected to heat-induced priming at 35 °C for 40 min exhibited a significant increase in germination from 32 to 65 %, compared to non-primed conidia. The primed conidia also demonstrated a reduction in the LT<sub>50</sub> by 10 h in comparison to their non-primed counterparts. While priming improved germination, it did not significantly affect radial growth or sporulation rates under heat stress. We further explored the memory time of priming, finding that the fungus retained the priming state for up to 4 h. To elucidate the molecular mechanisms underlying the heat stress response, we identified candidate metabolites using LC-MS. Metabolomic analysis identified 34 metabolites, with significant alterations in metabolic pathways related to spermidine biosynthesis, coenzyme A synthesis, fungal wall development and various cellular process among primed conidia. These results underscored the complex metabolic adjustments in <em>B. bassiana</em> conidia, highlighting the potential of priming strategies to enhance fungal tolerance to environmental stresses and improve biocontrol efficacy in agricultural applications.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101607"},"PeriodicalIF":2.9,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144229902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Uncovering the morphological and phylogenetic diversity of mushrooms in pecan orchards in the Southwestern United States 揭示美国西南部山核桃果园中蘑菇的形态和系统发育多样性

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-30 DOI: 10.1016/j.funbio.2025.101608

McKenzie L. Stock , Richard J. Heerema , Jennifer J. Randall , Adriana L. Romero-Olivares , Samuel A. Belteton , Ciro Velasco-Cruz , Nicole Pietrasiak

{"title":"Uncovering the morphological and phylogenetic diversity of mushrooms in pecan orchards in the Southwestern United States","authors":"McKenzie L. Stock , Richard J. Heerema , Jennifer J. Randall , Adriana L. Romero-Olivares , Samuel A. Belteton , Ciro Velasco-Cruz , Nicole Pietrasiak","doi":"10.1016/j.funbio.2025.101608","DOIUrl":"10.1016/j.funbio.2025.101608","url":null,"abstract":"<div><div>Symbiotic relationships between pecan tree roots and mycorrhizal fungi may enhance drought and salt tolerance, nutrient absorption, and disease resistance in pecan (<em>Carya illinoinensis</em> (Wangenh.) K. Koch) orchards of the arid Southwestern United States. Saprotrophic fungi contribute to orchard ecosystem health by breaking down organic matter and enriching soil quality. However, we currently have limited, mostly anecdotal knowledge on mushroom-forming fungi in Southwestern pecan orchards, and studies in other regions primarily focus on ectomycorrhizal fungi. This study provides a novel mycological survey of mushroom-forming fungi (both ectomycorrhizal and saprotrophic) in pecan orchards across New Mexico, Arizona, West Texas, and California. In collaboration with pecan producers, sporocarps from 31 different fungal taxa were collected for morphological, phylogenetic, and ecological analyses. UNITE and NCBI databases aided in taxon identification to construct a maximum-likelihood phylogenetic tree. Common ectomycorrhizal taxa included <em>Scleroderma</em> species<em>, Pisolithus tinctorius,</em> and <em>Tuber lyonii</em><em>.</em>Saprotrophs included <em>Chlorophyllum molybdites</em><em>,</em> the regionally-unique <em>Agaricus deserticola</em>, and others. Logistic regression revealed bare soil and tree row location as significant predictors for mycorrhizal fungi presence. This study offers an inaugural characterization of mushroom-forming fungal communities in Southwestern pecan orchards and highlights their potential roles in enhancing orchard ecosystem health and resilience.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101608"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Differential profiling of viable fungi in the gut of Penaeus vannamei cultured in brackishwater ponds with high protein, low protein and azolla-based diets 在高蛋白、低蛋白和以氮唑为基础饲料的咸淡水中养殖的凡纳滨对虾肠道内活菌的差异分析

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-29 DOI: 10.1016/j.funbio.2025.101595

N.S. Sudheer , I.F. Biju , L. Priyadarshini , D. De , T.K. Ghoshal , Babita Mandal , S. Das

{"title":"Differential profiling of viable fungi in the gut of Penaeus vannamei cultured in brackishwater ponds with high protein, low protein and azolla-based diets","authors":"N.S. Sudheer , I.F. Biju , L. Priyadarshini , D. De , T.K. Ghoshal , Babita Mandal , S. Das","doi":"10.1016/j.funbio.2025.101595","DOIUrl":"10.1016/j.funbio.2025.101595","url":null,"abstract":"<div><div>An understanding of the gut microflora of an organism provides vital information that could be used in developing strategies for health management. In this view, a differential profiling of fungi in the gut of <em>Penaeus vannamei</em> fed on high protein, low protein and azolla-based diets are carried out. Shrimp samples were collected from brackish water ponds maintained on various dietary changes and fungus species were isolated and identified. A total of 43 fungi isolates belonging to 29 species, 2 phyla, 8 classes, 11 orders, 22 families and 23 genera ware identified. Among the identified species 74.41 % are represented by phylum Ascomycota and 25.60 % of the species are represented by Basidiomycota. <em>Sakaguchia oryzae</em> (9 %) and <em>Parasarocladium breve</em> (9 %) were the dominant species. The data indicate that higher fungal diversity was present in low protein administered groups followed by high protein feed with a slight difference. The lowest fungal diversity was in the azolla-based diet administered group. <em>P. breve</em> and <em>Penicillium osxalicum</em> were abundant in the high protein feed administered groups. <em>S. oryzae</em> and <em>Acremonium brachypenium</em> ware abundant in low protein and azolla-based feed groups, respectively. The genera Acremonium (4.3 %) was found common in all tested diet administered groups. The results indicated that dietary changes may influence the fungal diversity in the gut of <em>P. vannamei</em>. Our study provides valuable information on the gut mycobiota of <em>P. vannamei</em> cultured on dietary changes through employing modern data analysis and visualization tools, which is essential for developing an effective management strategy to improve shrimp culture.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101595"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of fungal volatile organic compounds for enhancing plant growth and controlling plant pathogens 真菌挥发性有机化合物在促进植物生长和控制植物病原体中的应用

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-28 DOI: 10.1016/j.funbio.2025.101606

Elsherbiny A. Elsherbiny , Sabrien A. Omar , Joan W. Bennett

{"title":"Application of fungal volatile organic compounds for enhancing plant growth and controlling plant pathogens","authors":"Elsherbiny A. Elsherbiny , Sabrien A. Omar , Joan W. Bennett","doi":"10.1016/j.funbio.2025.101606","DOIUrl":"10.1016/j.funbio.2025.101606","url":null,"abstract":"<div><div>Volatile organic compounds (VOCs) play important and diverse roles in the environment. In complex ecological communities, they mediate inter-organismal communication. The physical properties of VOCs enable them to diffuse through the air and impact above-ground plant parts, as well as affect underground plant parts in gas-filled pores in the soil. Many microbial VOCs stimulate plant growth and/or inhibit the growth of plant pathogens. Because VOCs do not rely on direct contact between the biocontrol organism and the pathogen, they can work at a distance. However, VOCs easily dissipate into the atmosphere, and the physiological effects of VOCs are highly concentration-dependent, which pose challenges for controlled applications. Currently, their greatest utility is for storage pathogens and greenhouse crops. This review summarizes important findings about fungal VOCs from an agricultural perspective, giving examples of plant growth promotion and the inhibition of plant pathogens while also highlighting the challenges that accompany attempts to harness VOCs activity for agricultural productivity.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101606"},"PeriodicalIF":2.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Overview of scientific productivity in the field of fungal stress research 真菌胁迫研究领域科学生产力综述

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-24 DOI: 10.1016/j.funbio.2025.101598

Alene Alder-Rangel , Drauzio E.N. Rangel

{"title":"Overview of scientific productivity in the field of fungal stress research","authors":"Alene Alder-Rangel , Drauzio E.N. Rangel","doi":"10.1016/j.funbio.2025.101598","DOIUrl":"10.1016/j.funbio.2025.101598","url":null,"abstract":"<div><div>The study of fungal stress (FS) responses contributes to a deeper understanding of this important group of organisms. This bibliometric study explores what research has been conducted on how agricultural, environmental, and industrial important fungi respond to changes or stress in their environment. The Web of Science was employed to find publications related to FS tolerance, and after sorting, 3126 articles were analyzed. The major fungus/fungi studied in each article were noted and sorted into categories of Environmental, Industrial, or Agricultural FS. The publications were then assigned into these categories or General, for articles covering many fungi. Agricultural Fungi were further divided into Plant-pathogenic, Insect-pathogenic, Antagonist, Postharvest decay, and Nematode-trapping niches. Seventy years of research into FS were found, with the majority of publications after 1990, peaking in 2022. Almost half of the FS publications were about Agriculture, and plant pathogens were the most common niche studied. The most studied fungi were <em>Beauveria bassiana, Saccharomyces cerevisiae</em>, and <em>Aspergillus nidulans</em>. Studies on FS were conducted in 87 countries by at least 9766 researchers. Most of the top institutions and researchers in FS publications are Chinese.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101598"},"PeriodicalIF":2.9,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of phenotype and secondary metabolic silencing gene clusters in Aspergillus sydowii by velvet transcription factors 丝绒转录因子对西多曲霉表型和次生代谢沉默基因簇的调控

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-23 DOI: 10.1016/j.funbio.2025.101605

Jie Zeng , Yu Cao , Yihan Guo , Dongyou Xiang , Jiale Wang , Qingrui Xu , Xiaoqiang Lang , Hui Xu , Yi Cao

{"title":"Regulation of phenotype and secondary metabolic silencing gene clusters in Aspergillus sydowii by velvet transcription factors","authors":"Jie Zeng , Yu Cao , Yihan Guo , Dongyou Xiang , Jiale Wang , Qingrui Xu , Xiaoqiang Lang , Hui Xu , Yi Cao","doi":"10.1016/j.funbio.2025.101605","DOIUrl":"10.1016/j.funbio.2025.101605","url":null,"abstract":"<div><div>The silencing of biosynthetic gene clusters (BGCs) is a widespread phenomenon in fungal secondary metabolism. Traditional environmental induction strategies often fail to effectively activate these silent BGCs, whereas global transcription factor regulation has emerged as a powerful strategy for their activation. Therefore, this study focuses on Velvet family transcription factors VosA and VelB, investigating their functions in <em>Aspergillus sydowii</em> H-1 and their regulatory role in activating silent gene clusters. Phenotypic analyses revealed that <em>AsvelB</em> influence conidiation, colony morphology. Disruption or overexpression of <em>AsvosA</em> or <em>AsvelB</em> resulted in impaired cell wall integrity (CWI). Furthermore, the knockout strains Δ<em>AsvelB</em> and Δ<em>AsvosA</em> exhibited elevated intracellular reactive oxygen species (ROS) levels. These findings highlight their roles in maintaining cellular homeostasis and fungal physiology. Semi-quantitative PCR analysis of 19 type I polyketide synthase (PKS) genes revealed that <em>AsvosA</em> and <em>AsvelB</em> exert global regulatory effects on secondary metabolism in H-1. <em>EVM0012129.1</em> was identified as a candidate PKS gene highly associated with purple pigment biosynthesis through semi-quantitative PCR and qPCR analyses. Overexpression of <em>AsvosA</em> enhanced purple pigment production specifically in conidia and upregulated <em>EVM0012129.1</em>, further supporting its critical role in pigment synthesis. These findings provide new insights into the regulatory network of <em>AsvosA</em> and <em>AsvelB</em>, highlighting their potential applications in fungal biology and metabolic engineering.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101605"},"PeriodicalIF":2.9,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of Sti1, a homolog to mammalian Hop, in conidiation, environmental adaptation, and virulence of Beauveria bassiana 与哺乳动物Hop同源的Sti1在球孢白僵菌的条件、环境适应和毒力中的作用

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-20 DOI: 10.1016/j.funbio.2025.101596

Xingbing Liu , Ruixuan Li , Yuju Liu , Mingguang Feng , Gang Zhou , Jie Wang

{"title":"Roles of Sti1, a homolog to mammalian Hop, in conidiation, environmental adaptation, and virulence of Beauveria bassiana","authors":"Xingbing Liu , Ruixuan Li , Yuju Liu , Mingguang Feng , Gang Zhou , Jie Wang","doi":"10.1016/j.funbio.2025.101596","DOIUrl":"10.1016/j.funbio.2025.101596","url":null,"abstract":"<div><div>The stress-induced auxiliary chaperone protein Sti1, a homolog to mammalian Hop, played important but differential roles in environmental adaptation and virulence of various fungi, but the roles of Sti1 in filamentous entomopathogens are not well understood. Here, the roles of Sti1 were unveiled in <em>Beauveria bassiana</em>, a classical entomopathogen widely applied as fungal pesticides to insect control. The disruption of <em>sti1</em> led to a 68.88 % reduction in colony area at 33 °C and a 28.88 % decrease in median lethal time (LT<sub>50</sub>) of conidia at 45 °C, but demonstrated a comparable hyphal growth and conidial germination to the control strains (the wild-type strain and complementation mutants) under normal condition. Moreover, the deletion of <em>sti1</em> caused a reduction in conidiophores and then resulted in a 33.95–43.49 % decrease in conidiation after an incubation of 4–5 days. Additionally, the <em>sti1</em>-deficient mutants displayed impaired cell wall integrity and increased sensitivities to various stressors, including H<sub>2</sub>O<sub>2</sub>, menadione, NaCl, carbendazim, and UV-B radiation. Furthermore, the Δ<em>sti1</em> showed a 28.07 % delay in virulence to <em>Galleria mellonella</em> larvae via normal infection. These findings were consistent with the reduced production of glycerol, trehalose, mannitol, superoxide dismutases, and catalases. These findings indicate that Sti1 is important for conidiation, environmental adaptation, and pathogenicity of <em>B. bassiana</em>.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101596"},"PeriodicalIF":2.9,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144114954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the impact of apocarotenoids on pathogenic Fusarium oxysporum f.sp. lini and endophytic Fo47 strains 探讨类伪胡萝卜素对致病性尖孢镰刀菌的影响。lini和内生菌株Fo47

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-14 DOI: 10.1016/j.funbio.2025.101591

Yelyzaveta Kochneva , Marta Burgberger-Stawarz , Aleksandra Boba , Marta Preisner , Justyna Mierziak-Derecka , Anna Kulma

引用次数: 0

Microsclerotia of dark septate endophytic fungi from Paleogene and Neogene of Poland: fossil record and palaeoenvironmental significance of root fungal endophytes 波兰古近纪和新近纪暗隔内生真菌的微核：根真菌内生真菌的化石记录及其古环境意义

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-13 DOI: 10.1016/j.funbio.2025.101594

Grzegorz Worobiec , Elżbieta Worobiec , Marek Widera , Wojciech Granoszewski

引用次数: 0

Cell wall characteristics of Thielavia terrestris dispersed and pelleted mycelia 地蓟分散和球化菌丝的细胞壁特性

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-05-09 DOI: 10.1016/j.funbio.2025.101593

Olena Gromozova , Mara Grube , Peter Lytvyn , Maksym Kharchuk , Marina Fomina

{"title":"Cell wall characteristics of Thielavia terrestris dispersed and pelleted mycelia","authors":"Olena Gromozova , Mara Grube , Peter Lytvyn , Maksym Kharchuk , Marina Fomina","doi":"10.1016/j.funbio.2025.101593","DOIUrl":"10.1016/j.funbio.2025.101593","url":null,"abstract":"<div><div>In submerged fermentation of filamentous fungi, pelleted and dispersed types of growth are the two main morphological forms largely affecting the productivity of industrial processes. Despite the immense importance of fungal biotechnology various aspects of the biology of these two growth forms have been rarely studied. Whether there are any differences in architecture and properties of hyphal cell wall between pelleted and dispersed mycelia of the same non-genetically modified fungus was unknown. This comparative study characterized the cell wall structure and properties of pelleted and dispersed forms of <em>Thielavia terrestris</em> growth. Infrared spectroscopy revealed that the hyphal cell wall of pellets, in contrast to dispersed mycelium, contained considerably more chitin (22 % and 1–2 % of dry weight [dw], respectively), glucans (28 % and 10 % dw), and lipids (8 % and 6 % dw). The dispersed form was characterised by much higher content of phosphate-rich polysaccharide (dispersed vs pelleted: 23 % and 1 % dw) and protein (55 % and 40 % dw, respectively). TEM-microscopy showed the thicker cell wall of the dispersed form, which did not manifest the third well-defined electron-dense smooth outer layer present in pellets. The cell wall of the pelleted form was less susceptible to mechanical and enzymatic destruction and demonstrated in AFM hardness tests 5–10 times greater nano-hardness than the dispersed form. Thus, the cell wall structure and properties are very different in the pelleted than the dispersed growth forms, where the narrower and chitin/glucans-richer cell wall of the pelleted form provides more effective protection from mechanical and enzymatic damages.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 5","pages":"Article 101593"},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0