Frontiers in Plant Science最新文献

{"title":"Closed-loop systems for plants expressing animal proteins: a modernized framework to safeguard the future of agricultural innovation.","authors":"Kristin A Bresnahan, Justin M Ferber, J Thomas Carrato, Thomas J Stoddard, Patrick V Palad, Magi Richani","doi":"10.3389/fpls.2025.1426290","DOIUrl":"10.3389/fpls.2025.1426290","url":null,"abstract":"<p><p>Escalating population growth and climate change pressures on crop production necessitate agricultural innovation to ensure food security and sustainability. Plant molecular farming (PMF), which uses genetically modified (GM) plants to produce high-value proteins for food products, offers a promising solution. PMF products, particularly those that express an animal protein in seed and grain crops, have the potential to substantially benefit U.S. and global agriculture, food systems, economies, and the environment. Farmers can diversify and generate increased revenue streams, while consumers gain access to affordable proteins beyond those currently available. However, the development and commercialization of PMF products, especially those expressing allergenic animal proteins, require careful consideration of existing stewardship guidelines and best practices. Current GM plant stewardship practices must be thoroughly assessed to identify and address any gaps, ensuring that PMF products maintain identity preservation and containment throughout their lifecycle. Implementing a fit-for-purpose closed-loop system (CLS) is crucial for effectively identifying, managing, and mitigating the potential risks and liabilities associated with PMF product development, production, and processing. A CLS framework for PMF products expressing animal proteins should integrate existing best practices from Excellence Through Stewardship and applicable third-party guidelines, including by way of example ISO standards, Good Manufacturing Practices, Hazard Analysis and Risk-Based Preventive Controls, Hazard Analysis and Critical Control Points, and Safe Quality Food, as well as newly designed controls to address potential PMF-specific risks. This comprehensive approach maximizes containment, identity preservation, regulatory compliance, traceability, incident response capabilities, and continuous improvement across the product lifecycle. While customization is required based on each PMF product developer's specific product and operations, this paper examines industry best practices and describes CLS components that a PMF developer should consider in designing a robust, bespoke CLS to maintain identity preservation and product containment. Such a system will optimize for product quality and integrity while preventing commingling with commodity supplies and any associated market disruption while also addressing food safety. By implementing these rigorous systems, the PMF industry can realize its potential to contribute significantly to sustainable agriculture and food security.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1426290"},"PeriodicalIF":4.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11821629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The BASIDIN effector of the fungus <i>Moniliophthora perniciosa</i> promotes positive effects on the seed germination and seedlings development of <i>Lactuca sativa</i>.","authors":"Keilane Silva Farias, Monaliza Macêdo Ferreira, Ivina Barbosa De Oliveira, Ronaldo José Durigan Dalio, Carlos Priminho Pirovani","doi":"10.3389/fpls.2025.1529096","DOIUrl":"10.3389/fpls.2025.1529096","url":null,"abstract":"<p><p>Plant resistance inducers that activate plant defense mechanisms may be useful in reducing agrotoxic use. Lettuce is among the most economically important leafy vegetable crops in the world. Since lettuce propagates through seeds, the use of high-quality seeds is extremely important for establishing the crop. Several studies have demonstrated the potential of alternative methods of seed treatment with the aim of increasing productivity. Based on this premise, we tested the effect of the rBASIDIN effector regarding its ability to induce germination and physiological changes in lettuce seedlings through seed treatment. The seeds were treated for 30 min by soaking with 50 µg mL^-1, 75 µg mL^-1 and 100 µg mL^-1 of the recombinant effector protein rBASIDIN. Seeds treated with distilled water and 10 mmol of Tris-HCl served as controls. The physiological parameters evaluated were germination percentage at 4 and 7 days, seedling length (aerial part and root), dry and fresh mass, electrical conductivity, and enzymatic activity. Seeds treated with 50 and 75 µg mL^-1 of rBASIDIN germinated earlier than the controls. Treatment with rBASIDIN at a concentration of 50 µg mL^-1 resulted in seedlings with an average root length of 1.51 cm, while the average lengths of the controls (H₂O and buffer) were 0.86 and 0.70 cm respectively. Seed treatment with rBASIDIN caused an increase in the fresh and dry weight of the plants. The lowest electrolyte leakage was detected in seeds treated with the three concentrations of rBASIDIN compared to the controls. Regarding the activity of defense enzymes, seedlings treated with rBASIDIN at lower concentrations showed higher chitinase and β-glucanase activity compared to the controls. The results indicated that the rBASIDIN effector plays an important signaling role in lettuce seeds, since small doses are already sufficient to induce changes in physiological parameters to obtain more vigorous plants.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1529096"},"PeriodicalIF":4.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11821917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential responses of soil C, N, and P ecological stoichiometric characteristics to different configurations of edge-locked forests in the Kubuqi Desert.","authors":"Xue Chen, Hejun Zuo, Min Yan, Haibing Wang, Cheng Xi, Yinchao Chai","doi":"10.3389/fpls.2025.1520024","DOIUrl":"10.3389/fpls.2025.1520024","url":null,"abstract":"<p><p>As a vital component of the desert ecological protection system, the edge-locked forests of the Kubuqi Desert play a crucial role in mitigating wind erosion, stabilizing sand, maintaining soil and water, and restricting desert expansion. In this paper, six types of standard protection forests in the Kubuqi Desert, namely <i>Salix psammophila</i> (SL), <i>Elaeagnus angustifolia</i> (SZ), <i>Salix matsudana</i> (HL), <i>Corethrodendron fruticosum+Salix psammophila</i> (YC + SL), <i>Caragana korshinskii + Populus simonii</i> (XYY + NT), and <i>Elaeagnus angustifolia + Salix matsudana</i> (SZ + HL), were investigated. Notably, the vertical differentiation patterns of soil carbon (C), nitrogen (N), phosphorus (P), and ecological stoichiometric ratios, as well as soil particle size features within the 0-100-cm soil layer under protection forests with different allocation modes, were systematically and comprehensively analyzed. The study's findings showed that: (1) Among the six configuration types, SZ, NT + XYY, and SL exhibited higher soil SOC and TN concentrations. Both soil SOC and TN content decreased with increasing soil depth, whereas soil TP content displayed no considerable variation among different stand types or soil depths. (2) Based on the N/P threshold hypothesis, N was the limiting nutrient element for the growth of edge-locked forests in the region. (3) The understory soils of different configurations of edge-locked forests mainly comprised sand. The silt and clay contents of SL and NT + XYY were substantially higher than those of the other four configurations. The vertical distribution patterns of particle size and parameter characteristics had variations. (4) Soil C, N, P, and stoichiometric characteristics are affected by vegetation type, soil depth, and soil texture. In conclusion, SZ and SL can be used as the dominant tree species in the edge-locked forests of the Kubuqi Desert, and the NT + XYY mixed forest configuration pattern displays the most apparent soil improvement effect. This study's findings offer a scientific reference and foundation for restoring vegetation and enhancing the ecological environment in desert regions. In addition, they provide a theoretical foundation for establishing and managing edge-locked forests.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1520024"},"PeriodicalIF":4.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11821981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The SEPALLATA-like gene <i>HrSEP1</i> in <i>Hippophae rhamnoides</i> regulates flower development by interacting with other MADS-box subfamily genes.","authors":"Di Cong, Xue Zhao, Chang Ni, Mengru Li, Luwen Han, Jianlin Cheng, Hongzhang Liu, Huijing Liu, Dan Yao, Shuying Liu, Guoshuang Chen","doi":"10.3389/fpls.2024.1503346","DOIUrl":"10.3389/fpls.2024.1503346","url":null,"abstract":"<p><p>MADS-box genes are classified into five categories: ABCDE, including <i>SEP1</i>, <i>SEP2</i>, <i>SEP3</i>, <i>SEP4</i>, and other homologous genes, which play important roles in floral organ development. In this study, the cDNA sequence of the <i>HrSEP1</i> gene was cloned by RT-PCR and confirmed that this gene belongs to the MADS-box gene family. In addition, subcellular localization experiments showed that the <i>HrSEP1</i> protein was localized in the nucleus. We verified the interaction of <i>HrSEP1</i> with <i>HrSOC1</i>, <i>HrSVP</i>, and HrAP1 using yeast two-hybrid and bimolecular fluorescence complementation assays. These genes jointly regulate the growth and development of floral organs. We also found a strong synergy between <i>HrSEP1</i> and <i>AP1</i> genes in sepals, petals, and stamens by transgenic methods and fluorescence quantitative PCR, suggesting that <i>HrSEP1</i> and <i>AP1</i> may co-regulate the development of these structures. In conclusion, the expression of <i>HrSEP1</i> has a certain effect on the development of floral organs, and these findings lay the foundation for further research on the biological functions of MADS transcription factors in <i>Hippophae rhamnoides</i>.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"15 ","pages":"1503346"},"PeriodicalIF":4.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How does light regulate plant regeneration?","authors":"Juan Han, Yapeng Li, Ye Zhao, Yuhan Sun, Yun Li, Zuodeng Peng","doi":"10.3389/fpls.2024.1474431","DOIUrl":"10.3389/fpls.2024.1474431","url":null,"abstract":"<p><p>Based on the totipotency and pluripotency of cells, plants are endowed with strong regenerative abilities. Light is a critical environmental factor influencing plant growth and development, playing an important role in plant regeneration. In this article, we provide a detailed summary of recent advances in understanding the effects of light on plant regeneration, with a focus on the fundamental processes and mechanisms involved in <i>de novo</i> shoot regeneration, somatic embryogenesis, and adventitious root formation. We focus on summarizing the effects of light intensity, light spectra, and photoperiod on these regeneration processes. Additionally, we propose the molecular mechanisms and regulatory networks underlying light-mediated plant regeneration. This article aims to deepen our understanding of the role of light in plant regeneration and to pave the way for future research on light-regulated regenerative processes in plants.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"15 ","pages":"1474431"},"PeriodicalIF":4.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep <i>R-gene</i> discovery in HLB resistant wild Australian limes uncovers evolutionary features and potentially important loci for hybrid breeding.","authors":"Jianyang Liu, Khushwant Singh, Matthew Huff, Christopher Gottschalk, Michael Do, Margaret Staton, Manjunath L Keremane, Robert Krueger, Chandrika Ramadugu, Chris Dardick","doi":"10.3389/fpls.2024.1503030","DOIUrl":"10.3389/fpls.2024.1503030","url":null,"abstract":"<p><p>Huanglongbing (HLB) is a devastating citrus disease that threatens the citrus industry worldwide. HLB is associated with the bacteria <i>Candidatus Liberibacter asiaticus</i> (CLas) and as of today, there are no tools for economically viable disease management. Several wild Australian limes have been identified to be HLB resistant and their resistance is hypothesized to be conferred by resistance genes (R-genes), which mediate pathogen-specific defense responses. The aim of this study was to gain insight into the genomic features of R-genes in Australian limes, in comparison to susceptible citrus cultivars. In this study, we used five citrus genomes, including three Australian limes (<i>Citrus australasica</i>, <i>C. glauca</i> and <i>C. inodora</i>) and two cultivated citrus species (<i>C. clementina</i> and <i>C. sinensis</i>). Our results indicate up to 70% of the R-genes were identified in the unannotated regions in the original genome annotation of each species, owing to the use of a R-gene specific pipeline. Surprisingly, the two cultivated species harbored 15.8 to 104% more R-genes than the Australian limes. In all species, over 75% of the R-genes occurred in clusters and nearly 80% were concentrated in three chromosomes (Chr3, 5 and 7). The syntenic R-gene based phylogenic classification grouped the five species according to their HLB-resistance levels, reflecting the association between these R-genes and their distinct Australian origins. Domain structure analysis revealed substantial similarities in the R-genes between wild Australian limes and cultivated citrus. Investigation of chromosomal sites underlying Australian specific R genes revealed diversifying selection signatures on several chromosomal regions. The findings in this study will aid in the development of tools for genome-assisted breeding for HLB-resistant varieties.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"15 ","pages":"1503030"},"PeriodicalIF":4.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11831368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GWAS identifies a polyembryony locus in mango: development of KASP and PACE markers for marker-assisted breeding.","authors":"Gul Shad Ali, Shamseldeen Eltaher, Jin Li, Barbie Freeman, Sukhwinder Singh","doi":"10.3389/fpls.2025.1508027","DOIUrl":"10.3389/fpls.2025.1508027","url":null,"abstract":"<p><p>Apomixis is a horticultural trait that enables clonal propagation of hybrids by producing asexual embryos from maternal cells in the ovule without meiosis. Many mango cultivars exhibit apomictic polyembryony, where one embryo develops from zygotic tissues and the rest from nucellar tissues, resulting in seedlings that are genetically identical to the mother tree. In <i>Mangifera indica</i> L. commercially important rootstocks are raised from apomictic seeds, which are then grafted with desired cultivars. Identifying molecular markers for polyembryony and understanding its genetics would facilitate introducing this trait in commercially important cultivars. In this report, genome-wide association studies were conducted on a diversity panel consisting of 42 polyembryonic and 42 monoembryonic <i>M. indica</i> cultivars using high-density single nucleotide polymorphism (SNP) markers. These studies revealed that the polyembryony locus is in a 360-kb region on linkage group 17 of the 'Alphonso' reference genome. This locus contains the <i>MiRWP/MiRKD4</i> gene, which codes for an RWP-RK domain-containing protein previously implicated in citrus apomixis. Comparative genomic analyses revealed synteny between the citrus and the mango polyembryony loci, suggesting a common evolutionary mechanism for this trait. A total of 29 SNP markers in this locus were significantly associated with polyembryony in <i>M. indica</i>. Five of these markers were developed into convenient genotyping assays using competitive allele-specific PCR chemistry implemented in two different genotyping platforms - Kompetitive Allele-Specific PCR (KASP) and PCR allele competitive extension (PACE). The utility of these assays was validated and demonstrated in diverse germplasm collection and open-pollinated mango breeding populations with known pedigrees and polyembryony phenotypes. These SNP markers, especially those flanking the <i>MiRWP/MiRKD4</i> gene, provide a valuable tool for mango breeders to select polyembryonic progenies at the seedling stages in mango breeding programs.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1508027"},"PeriodicalIF":4.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11814187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143407028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of a one-step reverse transcription PCR detection method for <i>Tobamovirus maculatessellati</i>, in tomato (<i>Solanum lycopersicum</i> L.) and pepper (<i>Capsicum annuum</i> L.).","authors":"Chellappan Padmanabhan, Andrea Gilliard, Kai-Shu Ling, Yazmín Rivera","doi":"10.3389/fpls.2025.1535175","DOIUrl":"10.3389/fpls.2025.1535175","url":null,"abstract":"<p><p>The solanaceous-infecting tobamoviruses are closely related and hence it can be challenging to detect them using serological or molecular methods, particularly when present in a mixed infection. Tomato mottle mosaic virus (ToMMV) is a newly identified tobamovirus that poses serious risk to tomato (<i>Solanum lycopersicum</i> L.) and pepper (<i>Capsicum annuum</i> L.) production worldwide. Species-specific identification is crucial to prevent the entry and establishment of plant pathogens and protect the billion-dollar tomato industry. In this study, we report the validation of a previously described reverse transcription polymerase chain reaction (RT-PCR) assay that amplifies a 289 bp fragment of the coat protein coding region of ToMMV genome. This assay has 100% specificity for ToMMV. Inclusivity tests were performed against a diverse collection of six ToMMV isolates in North America. Exclusivity tests showed no cross reaction with eleven non-target viruses and seven viroids commonly found on tomato and pepper host plants. The detection limit of the one-step RT-PCR was determined to be at 10^-5 (or 0.25pg/μl) dilution in plant samples, with its amplicon sequence confirmed by Sanger sequencing. The RT-PCR can detect ToMMV consistently on contaminated seed or leaf tissues. This validated assay could serve as a standard method for detecting ToMMV in seed health testing and for plant disease diagnosis, thus to prevent inadvertent introduction and spread of this emerging and economically important tobamovirus in tomato and pepper fields.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1535175"},"PeriodicalIF":4.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143407051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of somatic embryogenesis and cryopreservation of <i>Abies pinsapo</i> Boiss.","authors":"Laura Cabero-Moreno, Ignacio Landeras-López, María Victoria Bravo-Navas, Carolina Sánchez-Romero","doi":"10.3389/fpls.2025.1535113","DOIUrl":"10.3389/fpls.2025.1535113","url":null,"abstract":"<p><p><i>Abies pinsapo</i> is an endangered species, endemic to southern Spain. Somatic embryogenesis and cryopreservation constitute important biotechnological tools, which can be used in order to improve the management and conservation of threatened species. The objective of this work was to develop somatic embryogenesis and cryopreservation protocols for <i>A. pinsapo</i>. Somatic embryogenesis was induced from mature zygotic embryos of <i>A. pinsapo</i> cultured on solid MS medium with macroelements at half-strength and supplemented with 20 g L^-1 sucrose and 5 mg L^-1 6-benzylaminopurine (BA). Embryogenic cultures successfully proliferated on solid medium with the same formulation supplemented with 20 g L^-1 sucrose, 500 mg L^-1 L-glutamine, 1 g L^-1 casein hydrolysate and 1 mg L^-1 BA. Different preconditioning and cryoprotective treatments were tested in order to optimize cryopreservation of embryogenic tissues by using the slow-cooling technique. Embryogenic cultures at their exponential growth phase, i.e. 12-14 days after the last subculture, were used as cryopreservation explants. The best results were achieved after sucrose preculture and cryoprotecion with PGD I (mixture of polyethylene glycol, glucose and DMSO I), with 100% of explants resuming somatic embryogenesis after thawing. Following fluorescein diacetate (FDA) staining, more intense and abundant green fluorescence could be observed in these samples, compared to those subjected to other preconditioning and cryoprotective treatments, thus evincing a higher proportion of viable cells after freezing in liquid nitrogen. Cold hardening did not improve cryotolerance. In fact, incubation at 5 °C for two weeks appeared to affect explants response, delaying tissue regrowth after cryopreservation. This is the first time in which somatic embryogenesis and cryopreservation have been reported in Spanish fir. The results obtained allow to establish the bases for the integration of these techniques into <i>in situ</i> and <i>ex situ</i> conservation strategies.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1535113"},"PeriodicalIF":4.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143407030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of <i>OsDUF868.12</i> enhances salt tolerance in rice.","authors":"Hao Chen, Jiale Wan, Jiali Zhu, Ziyi Wang, Caiyao Mao, Wanjing Xu, Juan Yang, Yijuan Kong, Xiaofei Zan, Rongjun Chen, Jianqing Zhu, Zhengjun Xu, Lihua Li","doi":"10.3389/fpls.2025.1458467","DOIUrl":"10.3389/fpls.2025.1458467","url":null,"abstract":"<p><p>Excessive salt accumuln in soil is one of the most important abiotic stresses in agricultural environments. The Domain of Unknown Function 868 (DUF868) family, comprising 15 members in rice, has been identified in the protein family database. In this study, we cloned and functionally characterized <i>OsDUF868.12</i>, a member of the OsDUF868 family, to elucidate its role in rice response to salt stress. A series of experiments, including RT-qPCR, Agrobacterium-mediated transient transformation in tobacco for localization analysis, phenotypic characterization, physiological and biochemical index measurement, and leaf staining, were conducted to investigate the function of <i>OsDUF868.12</i> under salt stress. Transcriptional analysis revealed that <i>OsDUF868.12</i> exhibited the most significant response to low temperature and salt stress. Preliminary subcellular localization studies indicated that <i>OsDUF868.12</i> is localized in the cell membrane. Phenotypic Identification Experiments showed Overexpression lines of <i>OsDUF868.12</i> enhanced resistance to salt stress and increased survival rates, while knockout lines of <i>OsDUF868.12</i> were opposite. Physiological and biochemical assessments, along with leaf staining, demonstrated that overexpression of <i>OsDUF868.12</i> improved the activity against oxidative stress.under salt stress. Furthermore, overexpression of <i>OsDUF868.12</i> elevated the transcription levels of positively regulated salt stress-related genes. These findings suggest that overexpression of <i>OsDUF868.12</i> enhances rice tolerance to salt stress at the molecular level through a series of regulatory mechanisms. This study provides valuable insights into the functional roles of the DUF868 family in plant responses to abiotic stress.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1458467"},"PeriodicalIF":4.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11814167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143407032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}