Frontiers in Plant Science最新文献

{"title":"Soil and plant communities co-regulating plant biomass allocation patterns along a saline-alkali gradient, case study of <i>Allium ramosum</i> in Songnen Grassland, Northeast China.","authors":"Changxing Fu, Jiayan Fan, Gaohua Fan, Heqi Wang, Congwen Wang, Wei Qiang, Dafu Yu, Yingxin Huang","doi":"10.3389/fpls.2025.1627304","DOIUrl":"10.3389/fpls.2025.1627304","url":null,"abstract":"<p><strong>Aims: </strong>Plants have developed sophisticated mechanisms to adapt to changing environments. The strategies for biomass allocation is critical for plant to ensure fitness. Increasing soil salinity has a dramatic impact on plant growth and reproduction from the individual to community level. However, our understanding of how plants adapt their biomass allocation strategies to changes in soil salinity and community structure is incomplete.</p><p><strong>Methods: </strong>We investigated 122 individuals of perennial herb <i>Allium ramosum</i> from 70 plots along a soil gradient in Songnen grassland. Field investigations determined the physicochemical properties of the soil, aboveground biomass, richness and individual and each organ (root, stem, leaf, flower, and bulb) biomass of A. ramosum.</p><p><strong>Results: </strong>The results showed that plant community aboveground biomass and community-weighted height decreased as soil salinity increased. <i>A. ramosum</i> individual size decreased, the allometric exponents between reproductive organs (flowers) and storage organs (bulbs) also decreased, with more biomass allocated to flowers. However, this trend was indirectly influenced by salinization through a reduction in community weighted height (reducing light competition) and community aboveground biomass (altering competitive pressure for resources), rather than a direct response to soil salinity.</p><p><strong>Conclusions: </strong>This study highlights the complex interplay between community structure and individual plant adaptation strategies in response to environmental gradients, emphasizing the role of community-scale processes in regulating individual resource allocation.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1627304"},"PeriodicalIF":4.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12504303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the potential of biocrust microorganisms in agriculture: cyanobacteria and heterotrophic bacteria with plant growth-promoting properties.","authors":"Carlotta Pagli, Lisa Maggioli, Beatriz Roncero-Ramos, Eloisa Pajuelo, Miriam Muñoz-Rojas, Roberto Braglia, Antonella Canini, Yolanda Cantón","doi":"10.3389/fpls.2025.1659217","DOIUrl":"10.3389/fpls.2025.1659217","url":null,"abstract":"<p><strong>Introduction: </strong>Drylands are subject to multiple overlapping stresses, including high temperatures, drought, and salinity, along with soils that are low in organic matter and nitrogen. Hence, both agricultural practices and natural regeneration in these areas are hindered by poor plant establishment and growth. The use of plant growth-promoting (PGP) microorganisms has recently emerged as a promising strategy to enhance plant performance under these harsh conditions.</p><p><strong>Methods: </strong>In this context, the aim of this work was to isolate and screen the PGP properties of cyanobacteria and heterotrophic bacteria from biocrusts in arid soils, representing a highly unexplored niche of microorganisms with potential application in agriculture and ecological restoration. We determined key PGP traits, including phosphate and potassium solubilization, growth under nitrogen-free conditions, siderophore and auxin production, as well as protease, lipase, DNase, amylase, catalase, and cytochrome-C-oxidase activities.</p><p><strong>Results: </strong>Our results showed that, among the cyanobacteria analyzed, <i>Nostoc commune</i> CANT2, isolated from the province of Almería (Spain), exhibited the highest number of PGP properties, followed by <i>N. commune</i> AB55 (southern Sardinia, Italy) and <i>Trichocoleus</i> cf. <i>desertorum</i> CAU7 (Almería). Both strains AB55 and CANT2 are characterized by their production of exopolysaccharides (EPS). Regarding the heterotrophic bacterial strains, those with the best PGP properties were identified as <i>Peribacillus frigoritolerans</i> and <i>Bacillus atrophaeus</i> by 16S rRNA gene sequencing. Seed biopriming experiments with the model plant <i>Triticum aestivum</i> showed that application of <i>N. commune</i> CANT2, either alone or in combination with <i>P. frigoritolerans</i> 1E, enhanced vigor indices by up to 58% compared to the control.</p><p><strong>Discussion: </strong>These findings highlight the potential of combined microbial consortia with PGP activities as candidates for the development of biostimulants, offering a sustainable approach to improve plant growth and resilience in dryland agriculture.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1659217"},"PeriodicalIF":4.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"YOLOv8-FDA: lightweight wheat ear detection and counting in drone images based on improved YOLOv8.","authors":"Yuxuan Lin, Xiao Xiao, Haifeng Lin","doi":"10.3389/fpls.2025.1682243","DOIUrl":"10.3389/fpls.2025.1682243","url":null,"abstract":"<p><strong>Introduction: </strong>Wheat is a vital global staple crop, where accurate ear detection and counting are essential for yield prediction and field management. However, the complexity of field environments poses significant challenges to achieving lightweight yet high-precision detection.</p><p><strong>Methods: </strong>This study proposes YOLOv8-FDA, a lightweight detection and counting method based on YOLOv8. The approach integrates RFAConv for enhanced feature extraction, DySample for efficient multi-scale upsampling, HWD for compressed and accelerated model training, and the SDL loss for improved bounding box regression.</p><p><strong>Results: </strong>Experimental results on the GWHD dataset show that YOLOv8-FDA achieves a precision of 86.3%, recall of 77.5%, and mAP@0.5 of 84.9%, outperforming the original YOLOv8n by significant margins. The model size is 2.96MB with a computational cost of 8.3 GFLOPs, and it operates at 19.2 FPS, enabling real-time counting with over 97.5% accuracy using cross-row segmentation.</p><p><strong>Discussion: </strong>The proposed YOLOv8-FDA model demonstrates strong detection performance, lightweight characteristics, and efficient real-time capability, indicating its high practicality and suitability for deployment in real-world agricultural applications.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1682243"},"PeriodicalIF":4.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12504506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silicon application enhances drought resilience in buckwheat: a comparative study of three varieties.","authors":"Jiri Krucky, Vaclav Hejnak, Pavla Vachova, Aayushi Gupta, Jan Kubes, Marek Popov, Milan Skalicky","doi":"10.3389/fpls.2025.1635709","DOIUrl":"10.3389/fpls.2025.1635709","url":null,"abstract":"<p><p>This study evaluated the effects of foliar silicon (Si) application on physiological and biochemical traits in three buckwheat lines (La Harpe, Panda, Smuga) grown under optimal (control) and drought stress conditions. Plants were cultivated under controlled conditions with four treatments: Control (80% water availability), Drought (40%), Control + Si, and Drought + Si (0.5 mM Na₂SiO₃·9H₂O applied to foliage). Water stress significantly reduced relative leaf water content (RWC), osmotic potential (Ψ_s), photosynthetic pigments, and gas exchange parameters (<i>A</i>, <i>E</i>, <i>g_s</i> ) in all varieties. It also increased malondialdehyde (MDA), total flavonoid content (TFC), total antioxidant capacity (TAC), and 5-methylcytosine (5mC), while Fv/Fm remained unchanged, indicating sustained photosystem II activity. However, varietal differences were evident. La Harpe and Panda showed lower RWC, Ψ_s, <i>A</i>, <i>E</i>, and <i>g_s</i> under drought than Smuga. La Harpe had the highest MDA accumulation in roots, increased 5mC levels in leaves, and was the only line with decreased water use efficiency (WUE). Smuga exhibited the highest natural proline level and the strongest proline increase under drought. Foliar Si application reduced MDA and enhanced antioxidant activity (TFC, TAC) in both roots and leaves across all varieties, under both water regimes. The strongest antioxidant response was observed in La Harpe. Si also improved photosynthetic pigment levels, likely contributing to the protection of the photosynthetic apparatus under drought stress. Its effects on RWC, Ψ_s, and gas exchange under drought were variety-specific: La Harpe and Panda responded positively, while Smuga showed minimal changes. Group correlation analysis under drought showed that Smuga had the strongest positive correlations between plant health traits and stress responses, suggesting effective physiological coordination. Panda showed moderate, and La Harpe negative, correlations. After Si application, these relationships improved most in Smuga, moderately in Panda, and least in La Harpe. Overall, the results reveal clear genotype-specific responses to foliar-applied Si in buckwheat. Silicon improved antioxidant defenses, mitigated drought-induced oxidative stress, and supported physiological functions, particularly in Smuga. These findings support using Si as a promising tool to enhance drought resilience in buckwheat cultivation.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1635709"},"PeriodicalIF":4.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12503412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the potential for genomic selection to improve resistance to fusarium stalk rot in maize.","authors":"Hirenallur Chandappa Lohithaswa, B M Showkath Babu, Muntagodu Shreekanth Sowmya, Santhosh Kumari Banakar, Nanjundappa Mallikarjuna, Ganiga Jadesha, Mallana Gowdra Mallikarjuna, D C Balasundara, Pandravada Anand","doi":"10.3389/fpls.2025.1631408","DOIUrl":"10.3389/fpls.2025.1631408","url":null,"abstract":"<p><p>Fusarium stalk rot (FSR), caused by <i>Fusarium verticilliodes</i>, is a serious disease in maize. Resistance to FSR is complexly inherited. Thus, an investigation was carried out to predict and validate the genomic estimated breeding values (GEBVs) for FSR resistance. Three doubled haploid (DH) populations induced from F₁ and F₂ of the cross VL1043 × CM212 and F₂ of the cross VL121096 × CM202 were used in the current study. Six different parametric models (Genomic-Best Linear Unbiased Predictors (GBLUP), BayesA, BayesB, BayesC, Bayesian least absolute shrinkage and selection operator (BLASSO), and Bayesian Ridge Regression (BRR)) were employed to estimate the prediction accuracy. Further, the accuracy of predicted genomic estimated breeding value (GEBV) for FSR resistance was assessed using five-fold cross-validation and independent validation. The training population (TP) size and marker density were optimized by considering different proportions of training set (TS) and validation set (VS) and varying marker density from 40 to 100%. The estimates of descriptive statistics and genetic variability parameters, which include mean, standardized range, genetic variance, phenotypic and genotypic coefficients of variations, broad sense heritability, and genetic advance as per cent mean (GAM), were relatively higher in DH F₂s than those in DH F₁s. Prediction accuracies displayed an increasing trend with an increase in the proportion of training set size and marker density in all three DH populations. The TS:VS proportion of 75:25 in DH F₁ (VL1043 × CM212) and DH F₂ (VL121096 × CM202), and 80:20 in DH F₂ of VL1043 × CM212 resulted in greater prediction accuracy than other TS:VS proportions. Study of linkage disequilibrium (LD) decay pattern across all the populations indicated that the number of markers employed were sufficient to conduct a genomic prediction (GP) study in two DH F₂ populations of crosses VL1043 × CM212 and VL121096 × CM202. Prediction accuracies of 0.24 and 0.17 were recorded for FSR resistance in independent validation when DH F₂ of cross VL121096 × CM202 was used for validation and DH F₁ and DH F₂s from the cross VL1043 × CM212 as training sets. A significant positive correlation of FSR resistance between the DHs selected based on their GEBVs and those selected based on test cross performance indicated the efficiency of genomic prediction models.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1631408"},"PeriodicalIF":4.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12500717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive phenotyping of 1,807 Indian barnyard millet (<i>Echinochloa frumentacea</i> Link) accessions from Indian national genebank: unlocking diversity for core set development.","authors":"Badal Singh, Sushil Pandey, S Nivedhitha, Neelam Shekhawat, Mamta Singh, Balram Jat, Chithra Devi Pandey, D P Semwal, Lalit Arya, R K Gautam, G P Singh","doi":"10.3389/fpls.2025.1644491","DOIUrl":"10.3389/fpls.2025.1644491","url":null,"abstract":"<p><strong>Introduction: </strong>A comprehensive characterization of 1,807 barnyard millet (<i>Echinochloa frumentacea</i> Link.) accessions conserved in the Indian National Genebank (INGB) was conducted to assess genetic variability and develop a representative core set.</p><p><strong>Methods: </strong>Thirteen qualitative and ten quantitative traits were evaluated. Five core sets were created using Core Hunter 3, utilizing optimization approaches to enhance representativeness and diversity. Comparisons were made between the entire collection and the developed core sets using diversity indices, statistical parameters, correlation analysis, and principal component analysis (PCA).</p><p><strong>Results: </strong>Significant diversity was revealed across traits. Predominant qualitative traits included pyramidal inflorescence shape (89%), green inflorescence color (57%), and intermediate inflorescence compactness (46%). Plant height ranged from 72.36 to 213.96 cm, inflorescence length from 6.73 to 35.65 cm, and 1000-seed weight from 1.01 to 5.55 g, demonstrating a wide range of quantitative characteristics. High heritability values (82.08-94.42%) and substantial genetic advances highlighted their agronomic importance. Among the five cores, core set-3 comprising 271 accessions achieved the best balance of genetic diversity, trait representativeness, and low redundancy, with a variable rate of coefficient of variance (VR) of 110.41%, coincidence rate (CR) of 85.97%, and mean difference percentage (MD) of 30%. Shannon-Weaver diversity indices and evenness values confirmed superior diversity representation. Comparisons showed non-significant differences in means, variances, and frequency distributions for most traits between the core and entire collection. Correlation and PCA confirmed conservation of trait associations and genetic structure, with the first five principal components explaining 74.9% of total variance in the core set, closely aligning with the entire collection (70.8%).</p><p><strong>Conclusion: </strong>This study highlights the utility of the INGB barnyard millet core set as a valuable genetic resource for breeding programs. The core set provides opportunities for the effective use and preservation of barnyard millet genetic resources by improving access to genetically diverse and agronomically significant germplasm.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1644491"},"PeriodicalIF":4.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of simulated nibbling intensity on growth and physiological characteristics of <i>Caragana tibetica</i>.","authors":"Yumei Liang, Minghui He, Ruiting Jia, Qi Tian, Jiahuan Chen, Min Li","doi":"10.3389/fpls.2025.1625476","DOIUrl":"10.3389/fpls.2025.1625476","url":null,"abstract":"<p><p>As one of the typical sand-fixation and soil and water conservation shrub species in the desert steppe, the effects of simulated nibbling intensity on the growth and physiological characteristics of <i>Caragana tibetica</i> are unclear. To solve the problem, in this paper, <i>Caragana tibetica</i> with complete configuration, good growth status, close location, the same terrain, and basically consistent morphological structure in the test area were selected as the research objects. The artificial pruning method was adopted to simulate different intensities of animal nibbling. The field <i>in-situ</i> sampling and indoor index determination methods were used to determine its growth and physiological characteristics, so as to clarify the growth and physiological response characteristics of <i>Caragana tibetica</i> to simulated nibbling. The results showed that: (1) The higher the nibbling intensity, the greater the number of new branches and the length of new branches. (2) Different simulated nibbling intensities had significant effects on the physiological characteristics of <i>Caragana tibetica</i>, whose effects are through antioxidant enzymes, osmoregulatory substances, and malondialdehyde. (3) During the whole growing season of <i>Caragana tibetica</i>, the value of physiological characteristics increased first and then decreased, reaching the maximum value in July. (4) The growth characteristics and physiological characteristics of <i>Caragana tibetica</i> have different degrees of correlation. This paper clarifies the impact of simulated nibbling intensity on the growth and physiological characteristics of <i>Caragana tibetica</i>, which can provide an important theoretical basis and practical significance for promoting the sustainable development and utilization of the desert steppe.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1625476"},"PeriodicalIF":4.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of pyrogenic carbon on tomato root architecture and metabolites (ABA and proline) under drought stress.","authors":"Yuan Zhang, Rifat-Un- Nisa, Aansa Rukya Saleem, Waqar-Un- Nisa, Abubakr M Idris, Guo Yu, Muhammad Tayyab Sohail, Habib Ullah","doi":"10.3389/fpls.2025.1634455","DOIUrl":"10.3389/fpls.2025.1634455","url":null,"abstract":"<p><strong>Introduction: </strong>Drought stress severely threatens global agriculture by reducing crop productivity and compromising food security. Biochar derived from agricultural waste has emerged as a promising soil amendment to enhance plant resilience and mitigate drought impacts.</p><p><strong>Methods: </strong>This study evaluated the effects of walnut shell biochar (WS biochar) at 3% and 5% (w/w) application rates on tomato (<i>Solanum lycopersicum</i>) growth under severe (45% field capacity) and moderate (75% field capacity) drought conditions. The biochar was characterized for physicochemical properties, and its impact on root architecture, biomass accumulation, and stress-related hormonal responses was assessed through greenhouse pot trials.</p><p><strong>Results: </strong>WS biochar exhibited high conversion efficiency (58.8%), with favorable properties such as high fixed carbon content (98%) and porous macroporous structure enhancing soil water retention. The 5% biochar treatment increased plant height by 24%, improved leaf production, and mitigated a 92% biomass reduction under severe drought conditions. Root systems showed 30% longer primary roots and 25% higher lateral root density. Biochar treatments reduced oxidative stress markers, lowering proline accumulation by 18% and abscisic acid (ABA) levels by 22% under severe drought.</p><p><strong>Discussion: </strong>Walnut shell biochar effectively enhances tomato drought resilience by improving root development, biomass, and physiological stress responses. These improvements likely stem from enhanced soil water retention and modified hormonal signaling. The findings support WS biochar's potential as a sustainable, climate-smart amendment to improve crop performance in water-limited environments. Further field studies are recommended to confirm long-term benefits on soil health and yield.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1634455"},"PeriodicalIF":4.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Current advances in Fusarium wilt.","authors":"Mahyar Mirmajlessi, Inmaculada Larena, María Del Mar Guerrero, María Del Carmen Rodríguez-Molina","doi":"10.3389/fpls.2025.1683977","DOIUrl":"https://doi.org/10.3389/fpls.2025.1683977","url":null,"abstract":"","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1683977"},"PeriodicalIF":4.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12502726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of CP12 on the metabolome of cyanobacteria under fluctuating CO₂ conditions.","authors":"Stefan Lucius, Stéphanie Arrivault, Regina Feil, Luna Alvarenga-Lucius, Martin Hagemann","doi":"10.3389/fpls.2025.1674721","DOIUrl":"10.3389/fpls.2025.1674721","url":null,"abstract":"<p><p>All organisms that perform oxygenic photosynthesis fix inorganic CO₂ through the Calvin-Benson-Bassham (CBB) cycle, which is then converted into many organic compounds in associated pathways of primary carbon and nitrogen metabolism. Autotrophic CO₂ fixation is only possible in the light, while under dark conditions, phototrophs adopt a heterotrophic lifestyle using stored organic carbon reserves. The switch between autotrophic and heterotrophic lifestyles often involves the activation and inactivation of key enzymes by redox regulation, including the regulatory protein CP12. In the present study, we analyzed the primary metabolism of the model cyanobacterium <i>Synechocystis</i> sp. PCC 6803 under different CO₂ conditions in continuous light using targeted metabolomics. The comparison of wild type and a mutant with deleted CP12 showed that this regulatory protein is crucial for the acclimation of the metabolism when shifted for 1 h or 3 h from high to low CO₂. Especially 1 h after shift from high into low CO₂, many metabolites of the primary carbon and nitrogen metabolism showed a strong transient increase in the mutant Δ<i>cp12</i>. Moreover, distinct differences were also observed when the strains were grown for longer times at high or low CO₂ conditions. Collectively, our results show that the absence of CP12 not only affected the CBB cycle under diurnal conditions but also had a marked impact on glycogen catabolism and associated nitrogen metabolism in cyanobacteria exposed to different CO₂ conditions in continuous light.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1674721"},"PeriodicalIF":4.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12500658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}