Frontiers in Plant Science最新文献

{"title":"The effect of endophytic bacteria on the growth, medicinal quality, and rhizosphere soil environment of <i>Isatis indigotica</i> Fort.","authors":"Yuqin Hu, Liqiong Sun, Xiaofan Li, Min Yang, Xiaoqing Tang, Kangcai Wang","doi":"10.3389/fpls.2026.1821717","DOIUrl":"https://doi.org/10.3389/fpls.2026.1821717","url":null,"abstract":"<p><p>Plant growth-promoting endophytes (PGPE) can form a mutually beneficial symbiotic relationship with host plants, analyzing the ability of endophytic bacteria of <i>Isatis indigotica</i> to promote growth and improve the rhizosphere environment and exploring the influence of dominant endophytic bacteria on the structure of rhizosphere microbial communities. In this study, we evaluated the ability of the three endophytic bacteria strains by a field experiment. The single endophytic bacterial strain and combination of every two bacterial strains were used for irrigating the rhizosphere of <i>I. indigotica</i> four times, and related indicators and rhizosphere soil of <i>I. indigotica</i> were measured. We screened out the dominant treatment groups based on the total active biomass of <i>I. indigotica</i> and analyzed microbial diversity of rhizosphere soil in dominant treatment groups. The results showed that endophyte treatments had significant effects on growth and physiology of <i>I. indigotica</i>, in which T11-28 and BC00 had the most significant effect on the dry weight of the aboveground part and underground part, respectively. The endophyte treatments had different effects on the content of active ingredients, rhizosphere soil chemical properties, and enzyme activities of <i>I. indigotica</i>, with BC00 promoting indigo and indirubin in leaves most significantly and BV11 promoting epigoitrin in roots most effectively. Total active biomass was calculated as the product of active ingredient content and biomass per plant. Based on this parameter, BC00 was the dominant treatment group, and the analysis of the diversity of its rhizosphere soil flora revealed that BC00 was able to enrich <i>Methylobacillus</i>, <i>Alternaria</i>, and other plant-growth-friendly flora. In the comprehensive analysis, the treatments of three endophytic bacterial strains of <i>I. indigotica</i> had significant promotion effects on its growth physiology and active ingredients and had obvious improvement effects on the rhizosphere environment, among which BC00 had the best comprehensive effect, which was associated with alterations in the rhizosphere soil microbial community structure.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"17 ","pages":"1821717"},"PeriodicalIF":4.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13144005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147836872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From labs to field realities: a paradigm shift in rice salinity screening.","authors":"Joie Ramos, Mahender Anumalla, Margaret Catolos, Ma Teressa Sta Cruz, Sankalp Bhosale, Waseem Hussain","doi":"10.3389/fpls.2026.1812738","DOIUrl":"https://doi.org/10.3389/fpls.2026.1812738","url":null,"abstract":"","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"17 ","pages":"1812738"},"PeriodicalIF":4.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13147186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147836878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root growth and function in New Zealand pasture systems: a perspective on research needs, methods, and system integration.","authors":"Fernando J Roca Fraga, W P T Wijenayakage, Astrid Volder, Donita L Cartmill, Katherine N Tozer, Gercílio A Almeida, Daniel J Donaghy, Andrew D Cartmill","doi":"10.3389/fpls.2026.1813059","DOIUrl":"https://doi.org/10.3389/fpls.2026.1813059","url":null,"abstract":"<p><p>Understanding root growth and phenology is essential for improving the productivity, resilience, and sustainability of pasture-based systems. However, roots remain one of the most difficult components of plant systems to measure and monitor, particularly in managed, high-turnover pastures, such as those in New Zealand (NZ) dairy systems. As a result, root processes are often underrepresented in both experimental studies and pasture system models. This perspective paper identifies critical, but underdeveloped areas in root research, with particular focus on root phenology. Current studies are limited by insufficient temporal resolution, a lack of species- and cultivar-specific trait data in mixed swards, and weak integration of root dynamics into breeding programmes and farm system models. These constraints limit our ability to link root processes to pasture persistence, nutrient cycling, and climate resilience. To address this gap, we propose that root phenology should be treated as a dynamic functional trait that links plant responses to environmental and management drivers with ecosystem-level outcomes. This framing provides a conceptual foundation for integrating root dynamics into pasture research and modelling, particularly in systems subject to frequent defoliation and environmental variability. We further highlight opportunities arising from rapid advances in sensing technologies, automation, and data analytics, which enable continuous, high-resolution root monitoring systems at multiple scales. However, realising this potential requires integration of complementary measurement approaches and alignment with system-level research questions. In this context, NZ provides a unique platform for developing scalable, pasture-based root monitoring framework that integrates science, management and policy. We argue for a coordinated effort that bridges fundamental root biology with applied pasture management, supported by long-term datasets, methodological integration, and engagement with end users. Embedding root traits and phenological dynamics into the next generation of pasture models and decision-support tools will be critical for improving system performance and environmental outcomes. This perspective aims to stimulate a shift towards more integrated, temporally explicit approaches for studying root systems in pasture environments, with relevance to grazing system beyond NZ and across temperate regions.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"17 ","pages":"1813059"},"PeriodicalIF":4.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13143933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147836889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating UAV-based phenotyping strategies for <i>Megathyrsus maximus</i>.","authors":"Guilherme Francio Niederauer, Alexandre Hild Aono, Mateus Figueiredo Santos, Celina de Medeiros Ragalzi, Liana Jank, Anete Pereira de Souza","doi":"10.3389/fpls.2026.1798414","DOIUrl":"https://doi.org/10.3389/fpls.2026.1798414","url":null,"abstract":"<p><p>Effective high-throughput phenotyping is crucial for modern plant breeding, yet the optimal image acquisition parameters for UAV-based systems in forage crops remain poorly defined. We optimized UAV-based phenotyping methods for a <i>Megathyrsus maximus</i> biparental population, examining how ground sampling distance (GSD), environment, and harvest date affect the accuracy of RGB-derived digital traits in predicting yield and canopy height. Machine learning algorithms and mixed model analyses were applied to evaluate predictive power and heritability. Pixel count and Haralick's entropy showed strong correlations with conventional yield measurements, particularly in Environment 2, while most vegetative indices were poor predictors. Integrating machine learning substantially enhanced predictive power for green and dry matter yield (r > 0.80). For canopy height, machine learning models achieved correlations of 0.71 with ground truth measurements despite weak pairwise correlations. Mixed model analysis revealed high broad-sense heritability (0.7 < <i>H</i> ² < 0.87) for yield traits, pixel count, and entropy, while vegetative indices and canopy height showed greater environmental susceptibility. Moderate GSD resolutions (0.5-1.0 cm) consistently outperformed both very high (0.27 cm) and very low (1.5 cm) resolutions. Coincidence index analysis demonstrated 80% correspondence between top genotypes ranked by pixel count and conventionally measured dry matter yield. This study provides an optimized framework for UAV-based phenotyping in <i>M. maximus</i>, demonstrating that combining advanced digital traits with machine learning accurately predicts key agronomic traits and significantly enhances genotype selection efficiency in forage breeding programs.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"17 ","pages":"1798414"},"PeriodicalIF":4.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13143898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147836925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing growth efficiency and energy economics in vertical farming through dynamic reduction of blue light in lettuce baby leaf (<i>Lactuca sativa L</i>.).","authors":"Vivek Jadhav, Vito Aurelio Cerasola, Karin Nilsson, Marco Ghio, Michael Martin, Giuseppina Pennisi, Giorgio Gianquinto, Francesco Orsini","doi":"10.3389/fpls.2026.1827422","DOIUrl":"https://doi.org/10.3389/fpls.2026.1827422","url":null,"abstract":"<p><strong>Introduction: </strong>Vertical farming offers a sustainable solution for urban food production, but energy optimization remains a critical challenge, with nearly half of the electricity requirements dedicated to artificial lighting. Dynamic adjustment of blue and red light can reduce energy costs, as blue light is more energy-intensive, thereby lowering operating expenses and increasing profitability.</p><p><strong>Methods: </strong>This research investigates the effects of dynamic adjustment of blue and red light on lettuce (<i>Lactuca sativa</i>, cv. Danstar) plants. Four light treatments were tested, each maintaining a total photosynthetic photon flux density (PPFD) of 200 μmol m^-2 s^-1 under a 16-hour photoperiod: (1) RB3 (control, 150 μmol m^-2 s^-1 red and 50 μmol m^-2 s^-1 blue); (2) 25% blue (B) reduction with hourly alternation between control and 175 μmol m^-2 s^-1 red/25 μmol m^-2 s^-1 blue; (3) 38% B reduction with hourly cycling through RB3, 162/38, 175/25, and 188/12 μmol m^-2 s^-1 of red/blue light; and (4) 50% B reduction with hourly alternation between control and 200 μmol m^-2 s^-1 monochromatic red. Agronomical, physiological, and morphological data were collected weekly from 7, 14, and 21 days after transplanting.</p><p><strong>Results: </strong>While the 50% B dynamic treatment did not enhance overall crop performance compared to the RB3 control, 25% B and 38% B increased lettuce fresh yield by 50-60%, with dry weight remaining stable.</p><p><strong>Discussion: </strong>These responses indicate improved leaf hydration (reduced dry matter content) resulting in increased fresh marketable yield, improved light-energy use efficiency by up to 63% and reduced lighting costs by 40%, demonstrating that constant blue light at a fixed PPFD is not required for optimal growth. This approach may offer a viable strategy to reduce production costs and enhance sustainability in controlled environment agriculture.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"17 ","pages":"1827422"},"PeriodicalIF":4.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13143623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147836865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microscopic attributes of <i>Olea europaea</i> L. leaves and bioclimatology: the case of Galicia, western Iberian Peninsula.","authors":"Rafael Álvarez, Enrique Escarda-Castro, Miguel Munárriz, Ramón Álvarez-Esteban, Aitor Álvarez-Santacoloma, Sara Del Río","doi":"10.3389/fpls.2026.1783046","DOIUrl":"https://doi.org/10.3389/fpls.2026.1783046","url":null,"abstract":"<p><strong>Introduction: </strong>The olive tree (<i>Olea europaea</i> L.) is widely recognized for its capacity to persist under conditions of limited water availability, a feature often associated with leaf anatomical and histological traits.</p><p><strong>Methods: </strong>In the present study, microscopic differences were comparatively analyzed in fully expanded leaves of five olive varieties sampled in Galicia (Brétema, Carapucho, Cobrancosa, Hedreira, and Mansa Gallega) and cultivated under controlled greenhouse conditions. Using light microscopy and scanning electron microscopy, we quantified adaxial cuticle thickness, palisade chlorophyll parenchyma thickness, leaf blade thickness, stomatal density, trichome density, and trichome morphometric traits. Additional qualitative traits were also assessed, including the presence of raphides and trichosclereids, the size of intercellular spaces in the aerenchymatous parenchyma, starch occurrence, the degree of curvature of the distal leaf portion, and the thickness of the vascular bundle sheath in the midrib.</p><p><strong>Results: </strong>Significant intervarietal differences were detected, allowing the identification of two distinct clusters. Group I (Brétema and Carapucho) was characterized by greater cuticle and leaf blade thickness, higher trichome density, a thinner vascular bundle sheath, and smaller intercellular spaces. Group II (Cobrancosa, Hedreira, and Mansa Gallega) showed lower cuticle and leaf blade thickness, lower trichome density, a thicker vascular bundle sheath, and larger intercellular spaces.</p><p><strong>Discussion: </strong>These results indicate contrasting histological configurations that may reflect different patterns of environmental adjustment among varieties. The observed traits suggest that Group I displays a more xeromorphic anatomical organization, whereas Group II shows features consistent with comparatively less structurally buffered leaf environments.</p><p><strong>Conclusions: </strong>Based on these findings and following the global bioclimatic classification system of Rivas-Martínez, the most suitable bioclimatic areas in northwestern Iberia for cultivation of the studied olive varieties are proposed. This study highlights the histological variability of olive leaves and provides useful information for varietal selection in relation to different bioclimatic contexts, including future climate change scenarios.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"17 ","pages":"1783046"},"PeriodicalIF":4.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13144125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147836946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing fungal community shifts associated with <i>Amauromyza karli</i> Hendel (Diptera: Agromyzidae) infestation in quinoa.","authors":"Neha Panwar, Jane E Stewart, Jorge R Ibarra Caballero, Adrianna Szczepaniec","doi":"10.3389/fpls.2026.1741091","DOIUrl":"https://doi.org/10.3389/fpls.2026.1741091","url":null,"abstract":"<p><strong>Introduction: </strong>Fungal communities are central elements of phytobiomes, yet their roles in mediating plant-insect interactions remain poorly understood. Here, we addressed this knowledge gap in quinoa, which has recently suffered significant losses due to a stem-boring pest.</p><p><strong>Methods: </strong>We used culture-based isolation from stems and amplicon-based profiling of rhizosphere soils to characterize quinoa-associated fungi across six site-year combinations in Colorado and to relate community patterns to abundance of stem-boring fly <i>Amauromyza karli</i> Hendel (Diptera: Agromyzidae).</p><p><strong>Results: </strong>Eighteen stem endophytes dominated by Ascomycota were isolated. Soil sequencing resolved 23 core amplicon sequence variants detected across all site-years; the core was primarily Ascomycota, with <i>Fusarium</i> spp., <i>Alternaria</i> spp., and <i>Plectosphaerella</i> spp. comprising over half of relative abundance of the entire community. Alpha diversity (richness, Shannon, inverse Simpson) differed significantly among site-years, and beta-diversity analyses revealed clustering by site and year. Abundance of adult <i>A. karli</i> was correlated positively with soil fungal richness and Shannon diversity and was also significantly associated with differences in community composition. Indicator and differential-abundance analyses identified taxa linked to low fly abundance (e.g., <i>Cladosporium herbarum</i>, <i>Alternaria</i> spp.) versus high abundance (e.g., <i>Fusarium</i> solani, <i>Microdochium</i> spp.). <i>Fusarium</i> spp., and <i>Alternaria</i> spp. were more prevalent in fields with high larval abundance, whereas antagonistic endophytes such as <i>Gibellulopsis piscis</i> and <i>Heydenia</i> spp. dominated in low-abundance fields.</p><p><strong>Discussion: </strong>These results indicated that community composition impacted pest pressure, with pathogenic fungi coinciding with higher fly abundance and entomopathogenic fungi enriched where larval pressure was lower. These findings identify candidate taxa for microbiome-informed integrated pest management and underscore the potential of site-specific practices (e.g., intercrops, organic amendments) to foster fungal communities that enhance quinoa resilience.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"17 ","pages":"1741091"},"PeriodicalIF":4.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13144115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Climate indirectly modulates tree survival of spruce beetle attacks via effects on constitutive and induced secondary metabolites.","authors":"Ehsan Khedive, Saeideh Fathi Moghanloo, Thomas Seth Davis","doi":"10.3389/fpls.2026.1801237","DOIUrl":"https://doi.org/10.3389/fpls.2026.1801237","url":null,"abstract":"<p><p>Despite many years of research, it remains challenging to determine why some trees survive bark beetle attacks while others do not. Current theory suggests that survival is predicated by interactions between tree defense systems and climatic drivers, but such interactions are poorly resolved at the scale of individual trees. Using a widespread conifer species (Engelmann spruce, <i>Picea engelmannii</i>) and a lethal phloem-feeding herbivore (North American spruce bark beetle, <i>Dendroctonus rufipennis</i>) as a study system, we characterized tree responses to mass attack across five populations representing a climate gradient in a field experiment. Over the course of a growing season, we measured variation in monoterpene concentrations in the phloem of 100 trees, half of which were challenged with mass attack using pheromone baits. Study trees were visited two years after baiting to record survival, and match survival to constitutive and induced monoterpenes. Our results revealed that regional climate correlated with constitutive, pre-attack concentration of monoterpenes in spruce phloem: a 1 kPa increase in vapor pressure deficit correlated with a doubling of monoterpene concentration. Higher constitutive phloem monoterpene concentrations predicted tree survival: a one-fold increase in concentration was associated with a three-fold increase in the odds of survival. Regional climate did not significantly affect the magnitude of induced response; however, early induction of monoterpenes in phloem distal to attack sites was associated with an average of twofold increase in survival odds, suggesting rapidity of monoterpene induction is a key resistance trait. These findings extend our understanding of climate-defense relationships in this system and indicate that Engelmann spruce tree populations in warmer and drier sites may have traits associated with higher constitutive resistance.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"17 ","pages":"1801237"},"PeriodicalIF":4.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13143916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147836529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomic and metabolomic analysis reveals the central role of phenylpropanoid biosynthesis in pea resistance to powdery mildew.","authors":"Yuanli Li, Jianying Lu, Yang Shao, Chang Wang, Lijuan Zhang, Gengmei Min","doi":"10.3389/fpls.2026.1793693","DOIUrl":"https://doi.org/10.3389/fpls.2026.1793693","url":null,"abstract":"<p><p>Pea powdery mildew severely reduces crop yield and quality, yet the dynamic molecular and metabolic regulatory networks underlying resistance differences between different pea varieties remain poorly understood. Here, we profiled the defense landscape of susceptible (Longwan 3) and resistant (Longwan 5) pea varieties across three infection stages (0, 3, and 6 days post-inoculation) via integrated transcriptomic and metabolomic analyses. Multi-omics data revealed pronounced differences in metabolic reconfiguration (1754 metabolites identified) and transcriptional reprogramming (34566 genes annotated) between the two pea cultivars. Integrated Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) consistently identified the phenylpropanoid biosynthesis pathway as the core pathway driving powdery mildew resistance. The resistant variety exhibited sustained upregulation of key biosynthetic genes (e.g., phenylalanine ammonia-lyase and cinnamate 4-hydroxylase), which directly drove the accumulation of defensive metabolites including ferulic acid and sinapic acid. Although some gene-metabolite correlations were consistent, others (e.g., those involving β-glucosidase and peroxidase genes) reflected a complex, multi-layered regulatory network involving post-transcriptional regulation and metabolic feedback mechanisms. Our study advances our understanding of dynamic defense mechanisms in legumes, and offers novel molecular targets for enhancing powdery mildew resistance, as well as efficient markers for precision breeding of elite pea varieties.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"17 ","pages":"1793693"},"PeriodicalIF":4.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13144052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147836707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pyramiding of <i>QYr.cib-3AS</i> and <i>YrT14</i> enhances wheat resistance to stripe rust.","authors":"Jianming Hu, Jiasheng Cheng, Yunfang Li, Fangpu Han, Lei Zhang, Yu Wu","doi":"10.3389/fpls.2026.1802598","DOIUrl":"https://doi.org/10.3389/fpls.2026.1802598","url":null,"abstract":"<p><p>Wheat stripe rust, caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i>, is a globally prevalent, wind-borne fungal disease and remains one of the most destructive threats to wheat production. By combining BSA-seq and QTL mapping using the wheat 120K SNP array, we identified two genomic regions associated with stripe rust resistance. Among them, a QTL on the short arm of chromosome 3A, <i>QYr.cib-3AS</i>, was consistently detected across environments, explaining 20.10%-25.21% of the phenotypic variance and showing LOD of 2.57-3.58. <i>QYr.cib-3AS</i> was delimited to an interval flanked by dCAPS-78 and dCAPS-83. Additive-effect analysis showed that RILs pyramiding <i>QYr.cib-3AS</i> with <i>YrT14</i> increased stripe rust resistance by 77.20% relative to RILs lacking both <i>QYr.cib-3AS</i> and <i>YrT14</i>, indicating that the pyramiding strategy had a significant impact on stripe rust resistance and underscoring its importance for high-yielding cultivars with durable resistance. The candidate interval exhibited high collinearity. Among the 48 high-confidence genes annotated in this region, an integrated analysis of transcriptome data, functional annotation, and sequence variation suggested that <i>TraesCS3A03G0118200</i> and <i>TraesCS3A03G0119800</i> are key candidate genes underlying <i>QYr.cib-3AS</i>. Collectively, these findings provide a foundation for marker-assisted breeding and future cloning and functional characterization of stripe rust resistance genes, which may help accelerate the development of elite stripe rust-resistant wheat cultivars, thereby improving wheat resistance to this destructive pathogen.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"17 ","pages":"1802598"},"PeriodicalIF":4.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13143962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147836898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}