Frontiers in Plant Science最新文献

{"title":"Comprehensive genome-wide analysis of the GmFRIGIDA gene family in soybean: identification, characterization, and expression dynamics.","authors":"Song Yu, Yuxuan Wang, Wenwen Ren, Yisheng Fang, Leili Wang, Yifei Zhang, Chengyang Song, Xiao Luo","doi":"10.3389/fpls.2025.1536866","DOIUrl":"10.3389/fpls.2025.1536866","url":null,"abstract":"<p><strong>Background: </strong><i>Frigida</i> (<i>FRI</i>) genes are crucial for regulating flowering time in plants. While the biological importance of the <i>Frigida-like</i> (<i>FRL</i>) gene family has been recognized in <i>Arabidopsis</i>, a systematic analysis of these genes in soybean is lacking. Characterizing <i>FRL</i> genes in soybean will help uncover their roles in flowering regulation, offering valuable insights for improving soybean adaptation.</p><p><strong>Results: </strong>In this study, we identified 16 <i>Frigida</i> genes in soybean, naming them based on their relationship to the <i>FRL</i> genes in <i>Arabidopsis thaliana</i>. These genes are unevenly distributed across thirteen chromosomes. Phylogenetic analysis categorizes Frigida-like proteins from <i>Arabidopsis</i>, soybean, and rice into four distinct subfamilies (I-IV). Our findings indicate that eight GmFRLs arose from whole-genome duplication (WGD) events, alongside two tandem duplication events. Gene structure analysis confirmed that all GmFRL members contain Frigida domains. Additionally, promoter analysis revealed numerous cis-acting elements related to photoperiodic response, suggesting their significant role in soybean's light response mechanisms. RNA-seq data demonstrated variable expression levels of <i>GmFRL</i> genes across tissues, including flower, leaf, pod, and seed, and other tissues, while subcellular localization and qPCR analyses further support their vital role in light responsiveness in soybean.</p><p><strong>Conclusion: </strong>In summary, our comprehensive analysis offers valuable insights into the evolution and potential functions of <i>GmFRL</i> genes, emphasizing their significance in photoperiodic responses and establishing a foundation for further research on the GmFRL family.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1536866"},"PeriodicalIF":4.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing tuber yield and nutraceutical quality of potato by supplementing sunlight with LED red-blue light.","authors":"Antonio Pannico, Nafiou Arouna, Giovanna Marta Fusco, Piero Santoro, Antonio Giandonato Caporale, Rosalinda Nicastro, Letizia Pagliaro, Stefania De Pascale, Roberta Paradiso","doi":"10.3389/fpls.2025.1517074","DOIUrl":"10.3389/fpls.2025.1517074","url":null,"abstract":"<p><strong>Introduction: </strong>We investigated the influence of genetic material and light spectrum on plant performance of two cultivars of potato (<i>Solanum tuberosum</i> L.), 'Colomba' and 'Libra', grown in greenhouse, in the view of future plant cultivation in Space and terrestrial vertical farming and controlled environment agriculture under limiting light conditions.</p><p><strong>Methods: </strong>The effects of 100% natural light (CNT) and two lighting treatments, in which 30% of solar radiation was replaced by red and blue LED light, RB 1:1 and RB 2:1, were evaluated on plant growth, gas exchange, and tuber yield and quality.</p><p><strong>Results: </strong>In CNT plants, net photosynthesis (NP) was similar in the cultivars, while the aerial biomass and tuber yield were greater in 'Libra'. In 'Colomba', NP and plant leaf area were unaffected by lighting treatments, however tuber yield increased under RB 2:1. Conversely, in 'Libra' both the aerial biomass and tuber production decreased in RB 2:1. Tubers of 'Colomba' contained higher concentrations of most minerals than 'Libra', probably due to different genetic traits and the slightly lower biomass (concentration effect). Red-blue lighting did not alter the mineral content of tubers. 'Colomba' prioritized the accumulation of free amino acids, GABA, and polyphenols, enhancing the plant stress response and antioxidant capacity, and adapted well to variable light conditions, with significant increases in tuber yield under LED treatments. Differently, 'Libra' focused on synthesis of carbohydrates, and essential amino acid content was lower compared to 'Colomba'.</p><p><strong>Discussion: </strong>Our findings underline the importance of genotype selection and highlights how light spectrum can improve the plant performance in potato. This knowledge could be useful in controlled environment agriculture and indoor cultivation (i.e., vertical farming) as well as in space research on potato, as this crop is a candidate for plant-based regenerative systems for long-term missions.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1517074"},"PeriodicalIF":4.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931653/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"E-Nose and HS-SPME-GC-MS unveiling the scent signature of <i>Ligusticum chuanxiong</i> and its medicinal relatives.","authors":"Wanjing Xu, Chao Zhang, Rong Xu, Juan Yang, Yijuan Kong, Li Liu, Shan Tao, Yu Wu, Hailang Liao, Changqing Mao, Zhengjun Xu, Fang Peng","doi":"10.3389/fpls.2025.1476810","DOIUrl":"10.3389/fpls.2025.1476810","url":null,"abstract":"<p><strong>Introduction: </strong>To explore the origin and evolution of Ligusticum Chuanxiong, we conducted a component analysis of Ligusticum Chuanxiong and its medicinal relatives.</p><p><strong>Methods: </strong>This study encompassed seven species from various origins, including Chuanxiong (<i>Ligusticum chuanxiong</i> Hort.), Gansu Chuanxiong (<i>Ligusticum chuanxiong</i> cv. Gansu), Yunnan Chuanxiong (<i>Ligusticum chuanxiong</i> cv. Yunnan), Japanese Chuanxiong (<i>Cnidium officinale</i> Makino), Fuxiong (<i>Ligusticum sinense</i> 'Fuxiong'), Gaoben (<i>Ligusticum sinense</i>), and Liaogaoben (<i>Ligusticum jeholense</i>), comprising 27 distinct materials. We employed headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) to identify various odor profiles from these species using electronic nose technology (E-nose). The method effectively identified volatile constituents in the leaves of these seven species.</p><p><strong>Results: </strong>Results indicated that odor differences between <i>L. chuanxiong</i> and its medicinal relatives were predominantly observed in sensors W1W and W1S. Linear discriminant factor analysis (LDA) successfully distinguished five of the relatives; however, <i>L. chuanxiong</i> and <i>L. sinense</i> exhibited high odor similarity, limiting complete differentiation in some samples. HS-SPME-GC-MS identified a total of 118 volatile constituents, with eight differential volatiles identified: trans-Neocnidilide, β-Caryophyllene, β-Selinene, 5-Pentylcyclohexa-1,3-diene, (E)-Ligustilide, Butylphthalide, Neophytadiene, and Senkyunolide. Hierarchical cluster analysis (HCA) grouped <i>L. chuanxiong</i>, <i>L. sinense</i>, <i>L. jeholense</i>, and <i>L. chuanxiong</i> cv. Gansu together, highlighting the close relationship between <i>L. chuanxiong</i> and <i>L. sinense</i>. Joint analysis revealed a significant positive correlation between sensor W1W and the differential volatile component β-Caryophyllene, suggesting its potential for distinguishing closely related species.</p><p><strong>Discussion: </strong>This study provides a foundational understanding of volatile components in the leaves of <i>L. chuanxiong</i> and its medicinal relatives using E-nose combined with HS-SPME-GC-MS, contributing to the discussion on their interspecific odor characteristics and relationships.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1476810"},"PeriodicalIF":4.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining multispectral and high-resolution 3D imaging for leaf vein segmentation and density measurement.","authors":"Yi-Hong Liao, Song Zhang","doi":"10.3389/fpls.2025.1560220","DOIUrl":"10.3389/fpls.2025.1560220","url":null,"abstract":"<p><p>Accurate leaf vein segmentation and vein density (VLA) measurement are crucial for understanding plant physiology. Traditional 2D imaging techniques often require labor-intensive and destructive processes, such as leaf flattening or chemical clearing, thereby limiting their practicality for high-throughput applications. In this study, we present a novel framework that integrates multispectral and high-resolution 3D imaging to enhance leaf vein segmentation and VLA measurement. By leveraging digital fringe projection, our system captures grayscale, multispectral, and 3D topographical data within a unified coordinate system. The integration of 3D information improves vein detection, particularly in low-contrast regions, while also enabling direct and accurate measurements of leaf area, vein length, and VLA. However, this approach also introduces some false positives in vein segmentation due to mesophyll surface variability. Despite these challenges, our high-resolution 3D imaging method shows significant potential for non-invasive phenotyping and trait assessment in complex, unstructured environments.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1560220"},"PeriodicalIF":4.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Precision exploration of plant germplasm resources: breakthroughs and applications in telomere-to-telomere (T2T) genomics.","authors":"Longxin Wang, Kai-Hua Jia","doi":"10.3389/fpls.2025.1573144","DOIUrl":"https://doi.org/10.3389/fpls.2025.1573144","url":null,"abstract":"","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1573144"},"PeriodicalIF":4.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of endogenous hormones regulating gallic acid biosynthesis during the development of buds and leaves in tea plant (<i>Camellia sinensis</i>).","authors":"Yutao Shi, Xiaofeng Lu, Qingying Song, Huan Sun, Wujing Shen, Ruiqi Huang, Jiapeng Huang, Yanfen Wei, Fumin Xiang, Xi Wang, Yanming Tuo, Jinke Lin, Yunfei Hu","doi":"10.3389/fpls.2025.1553266","DOIUrl":"10.3389/fpls.2025.1553266","url":null,"abstract":"<p><p>Gallic acid (GA), as a precursor of Epigallocatechin-3-gallate (EGCG) biosynthesis in tea plant, is one of the important components of tea flavor and has various health benefits. However, the mechanism of endogenous hormones regulating GA biosynthesis during the development of buds and leaves of tea shoots is still unclear. In this study, the buds and leaves of five different developmental stages of tea shoots were used as test materials to explore the mechanism of endogenous hormone signaling pathway regulating GA biosynthesis. The results showed that the decrease of D-erythrosyl-4-phosphate content and the increase of shikimic acid content affected the accumulation of GA content during the development of tea shoots. Jasmonic acid, abscisic acid, auxin, cytokinin, and gibberellin inhibited GA biosynthesis by down-regulating the expression of two <i>CsaroDEs</i> through twenty-three plant hormone signal transduction factors, such as <i>CsMYC2</i>, <i>CsSNRK2, CsARR-A</i>, and <i>CsDELLA</i>, respectively, which mediated the downregulation of sixteen transcription factors, such as <i>CsMYB44</i>, <i>CsMYB108</i>, and <i>CsC2C2</i>. <i>CsMYC2</i> and <i>CsSNRK2</i> co-mediated the downregulation of the expression of <i>CsMYB44</i> and <i>CsMYB108</i> in response to changes in endogenous JA and ABA content, respectively, and inhibited the expression of <i>CsaroDE</i>, thereby co-regulating GA biosynthesis. <i>CsMYC2</i> may be a key interworking site for the endogenous Jasmonic acid and abscisic acid signaling pathways to jointly regulate GA biosynthesis. Our findings revealed the potential mechanism of endogenous hormones regulating GA biosynthesis during the development of buds and leaves of tea shoots and provided a scientific basis for the regulation of tea quality.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1553266"},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling of rhizosphere-associated microbial communities in North Alabama soils infested with varied levels of reniform nematodes.","authors":"Sowndarya Karapareddy, Varsha C Anche, Sowjanya R Tamatamu, Madhusudhana R Janga, Kathy Lawrence, Leopold M Nyochembeng, Antonette Todd, Lloyd T Walker, Venkateswara R Sripathi","doi":"10.3389/fpls.2025.1521579","DOIUrl":"10.3389/fpls.2025.1521579","url":null,"abstract":"<p><strong>Introduction: </strong>Plant roots, nematodes, and soil microorganisms have a complex interaction in the rhizosphere by exchanging or communicating through biomolecules or chemicals or signals. Some rhizospheric (including endophytic) microbes process such compounds via biogeochemical cycles to improve soil fertility, promote plant growth and development, and impart stress tolerance in plants. Some rhizospheric microbes can affect negatively on plant parasitic nematodes (PPNs) thus hindering the ability of nematodes in parasitizing the plant roots. Next-generation sequencing is one of the most widely used and cost-effective ways of determining the composition and diversity of microbiomes in such complex environmental samples.</p><p><strong>Methods: </strong>This study employed amplicon sequencing (Illumina/NextSeq) of 16S ribosomal RNA (16S rRNA) for bacteria and Internal Transcribed Spacer (ITS2) region for fungi to profile the soil microbiome in the rhizosphere of cotton grown in North Alabama. We isolated DNA (ZymoBIOMICS) from soil samples in triplicates from four representative locations of North Alabama. Based on the level of Reniform Nematode (RN) Infestation, these locations were classified as Group A-RN Not-Detected (ND), Group B-RN Low Infestation (LI), Group C-RN Medium Infestation (MI), and Group D-RN High Infestation (HI) and determined using sieving method and microscopic examination.</p><p><strong>Results and discussion: </strong>Our analyses identified 47,893 bacterial and 3,409 fungal Amplicon Sequence Variants (ASVs) across all groups. Among the bacterial ASVs, 12,758, 10,709, 12,153, and 11,360 unique ASVs were determined in Groups A, B, C, and D, respectively. While 663, 887, 480, and 326 unique fungal ASVs were identified in Groups A, B, C, and D, respectively. Also, the five most abundant rhizospheric bacterial genera identified were <i>Gaiella</i>, <i>Conexibacter</i>, <i>Bacillus</i>, <i>Blastococcus</i>, <i>Streptomyces</i>. Moreover, five abundant fungal genera belonging to <i>Fusarium, Aspergillus, Gibberella, Cladosporium, Lactera</i> were identified. The tight clustering of bacterial nodes in <i>Actinobacteria</i>, <i>Acidobacteria</i>, and <i>Proteobacteria</i> shows they are highly similar and often found together. On the other hand, the close association of <i>Ascomycota</i> and <i>Basidiomycota</i> suggesting that they have different ecological roles but occupy similar niches and contribute similar functions within the microbial community. The abundant microbial communities identified in this study had a role in nutrient recycling, soil health, plant resistance to some environmental stress and pests including nematodes, and biogeochemical cycles. Our findings will aid in broadening our understanding of how microbial communities interact with crops and nematodes in the rhizosphere, influencing plant growth and pest management.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1521579"},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on delayed flowering under short-day condition in <i>Arabidopsis thaliana</i>.","authors":"Yunhui Wang, Tianxiao Lv, Tian Fan, Yuping Zhou, Chang-En Tian","doi":"10.3389/fpls.2025.1523788","DOIUrl":"10.3389/fpls.2025.1523788","url":null,"abstract":"<p><p>Flowering represents a pivotal phase in the reproductive and survival processes of plants, with the photoperiod serving as a pivotal regulator of plant-flowering timing. An investigation of the mechanism of flowering inhibition in the model plant <i>Arabidopsis thaliana</i> under short-day (SD) conditions will facilitate a comprehensive approach to crop breeding for flowering time, reducing or removing flowering inhibition, for example, can extend the range of adaptation of soybean to high-latitude environments. In <i>A. thaliana</i>, CONSTANS (CO) is the most important component for promoting flowering under long-day (LD) conditions. However, CO inhibited flowering under the SD conditions. Furthermore, the current studies revealed that <i>A. thaliana</i> delayed flowering through multiple pathways that inhibit the transcription and sensitivity of FLOWERING LOCUS T (FT) and suppresses the response to, or synthesis of, gibberellins (GA) at different times, for potential crop breeding resources that can be explored in both aspects. However, the underlying mechanism remains poorly understood. In this review, we summarized the current understanding of delayed flowering under SD conditions and discussed future directions for related topics.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1523788"},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926150/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the extraction method of <i>Glycyrrhiza uralensis</i> Fisch. distribution area based on Gaofen-1 remote sensing imagery: a case study of Dengkou county.","authors":"Xinxin Wei, Zeyuan Zhao, Taiyang Chen, Xiaobo Zhang, Shuying Sun, Minhui Li, Tingting Shi","doi":"10.3389/fpls.2025.1517764","DOIUrl":"10.3389/fpls.2025.1517764","url":null,"abstract":"<p><p><i>Glycyrrhiza uralensis</i> Fisch., a perennial medicinal plant with a robust root system, plays a significant role in mitigating land desertification when cultivated extensively. This study investigates Dengkou County, a semi-arid region, as the research area. First, the reflectance differences of feature types, and the importance of bands were evaluated by using the random forest (RF) algorithm. Second, after constructing the <i>G. uralensis</i> vegetation index (GUVI), the recognition accuracy of <i>G. uralensis</i> was compared between the RF classification model constructed based on the January-December GUVI and common vegetation indices feature set and the support vector machine (SVM) classification model constructed on the GUVI feature set. Finally, the spectral characteristics of <i>G. uralensis</i> and other feature types under the 2022 GUVI feature set were analyzed, and the historical distribution of <i>G. uralensis</i> was identified and mapped. The results demonstrated that the blue and near-infrared bands are particularly significant for distinguishing <i>G. uralensis</i>. Incorporating year-round (January-December) data significantly improved identification accuracy, achieving a producer's accuracy of 97.26%, an overall accuracy of 93.00%, a Kappa coefficient of 91.38%, and a user's accuracy of 97.32%. Spectral analysis revealed distinct differences with <i>G. uralensis</i> of different years and other feature types. From 2014 to 2022, the distribution of <i>G. uralensis</i> expanded from the northeast of Dengkou County to the central and southwestern regions, transitioning from small, scattered patches to larger, concentrated areas. This study highlights the effectiveness of GUVI and RF classification models in identifying <i>G. uralensis</i>, demonstrating superior performance compared to models using alternative feature sets or algorithms. However, the generalizability of the RF model based on the GUVI feature set may be limited due to the influence of natural and anthropogenic factors on <i>G. uralensis</i>. Therefore, regional adjustments and optimization of model parameters may be necessary. This research provides a valuable reference for employing remote sensing technology to accurately map the current and historical distribution of <i>G. uralensis</i> in regions with similar environmental conditions.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1517764"},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of mediator subunit MED7 in Arabidopsis development.","authors":"Koppolu Raja Rajesh Kumar, Jeanette Blomberg, Stefan Björklund","doi":"10.3389/fpls.2025.1542950","DOIUrl":"10.3389/fpls.2025.1542950","url":null,"abstract":"<p><p>MED7, a middle-module subunit of the transcriptional co-regulator Mediator complex, plays a critical role in gene regulation in <i>Arabidopsis thaliana</i>, where it is encoded by two paralogs, <i>MED7A</i> and <i>MED7B</i>. We present phenotypic analyses of homozygous MED7-silenced transgenic lines with significantly reduced expression of both <i>MED7</i> paralogs under autotrophic conditions. Our findings demonstrate that MED7 is essential for proper cotyledon opening during de-etiolation, as the silenced lines showed a marked delay in this process. Additionally, these lines displayed distinct morphological alterations, including hyponastic cotyledons, elongated hypocotyls, and modified root architecture, such as shorter primary roots and impaired root hair development in light-grown seedlings. <i>MED7</i> silencing also significantly hindered light-induced adventitious root (AR) formation on the hypocotyls of etiolated seedlings, leading to a notable reduction in AR production. Moreover, <i>MED7</i> silencing impacted the timing of floral transition and shoot branching, resulting in delayed flowering and an increased number of primary cauline branches on the inflorescence stem. Together, these results underscore a central role for MED7 in orchestrating key developmental processes in plants.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1542950"},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}