General and comparative endocrinology最新文献

{"title":"Association of seasonal changes in circulating cortisol concentrations with the expression of cortisol biosynthetic enzymes and a glucocorticoid receptor in the blubber of common bottlenose dolphin","authors":"Miwa Suzuki ,&nbsp;Noriko Funasaka ,&nbsp;Yuki Sato ,&nbsp;Daiki Inamori ,&nbsp;Yurie Watanabe ,&nbsp;Miki Ozaki ,&nbsp;Masayuki Hosono ,&nbsp;Hideaki Shindo ,&nbsp;Keiko Kawamura ,&nbsp;Toshiyuki Tatsukawa ,&nbsp;Motoi Yoshioka","doi":"10.1016/j.ygcen.2024.114516","DOIUrl":"https://doi.org/10.1016/j.ygcen.2024.114516","url":null,"abstract":"<div><p>Cortisol is secreted from the adrenal cortex in response to stress, and its circulating levels are used as robust physiological indicators of stress intensity in various animals. Cortisol is also produced locally in adipose tissue by the conversion of steroid hormones such as cortisone, which is related to fat accumulation. Circulating cortisol levels, probably induced by cold stress, increase in cetaceans under cold conditions. However, whether cortisol production in subcutaneous adipose tissue is enhanced when fat accumulation is renewed during the cold season remains unclear. Therefore, in this study, we examine the effect of environmental temperature on the expression of cortisol synthesis-related enzymes and a glucocorticoid receptor in the subcutaneous fat (blubber) and explore the association between these expressions and fluctuations in circulating cortisol levels in common bottlenose dolphins (<em>Tursiops truncatus</em>). Skin biopsies were obtained seasonally from eight female dolphins, and seasonal differences in the expression of target genes in the blubber were analyzed. Blood samples were collected throughout the year, and cortisol levels were measured. We found that the expressions of cytochrome P450 family 21 subfamily A member 2 (CYP21A2) and nuclear receptor subfamily 3 group C member 1 (NR3C1), a glucocorticoid receptor, were increased in the cold season, and 11 beta-hydroxysteroid dehydrogenase type 1 (HSD11B1) showed a similar trend. Blood cortisol levels increased when the water temperature decreased. These results suggest that the conversion of 17-hydroxyprogesterone to cortisol via 11-deoxycortisol and/or of cortisone to cortisol is enhanced under cold conditions, and the physiological effects of cortisol in subcutaneous adipose tissue may contribute to on-site lipid accumulation and increase the circulating cortisol concentrations. The results obtained in this study highlight the role of cortisol in the regulation of the blubber that has developed to adapt to aquatic life.</p></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140545999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “The uterus masculinus of the Eurasian beaver (Castor fiber L.) – The appraisal of fast hormone regulation by membrane androgen and estrogen receptors involvement” [Gen. Comp. Endocrinol. 345 (2024) 114389]","authors":"M Kotula-Balak ,&nbsp;G. Lonc ,&nbsp;M. Zarzycka ,&nbsp;J. Tomiyasu ,&nbsp;K. Knapczyk-Stwora ,&nbsp;B.J. Płachno ,&nbsp;A.J. Korzekwa ,&nbsp;J. Kaczmarczyk ,&nbsp;I. Krakowska","doi":"10.1016/j.ygcen.2024.114502","DOIUrl":"10.1016/j.ygcen.2024.114502","url":null,"abstract":"","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0016648024000625/pdfft?md5=de6cc03924b297a2eeae71ac99efbd3a&pid=1-s2.0-S0016648024000625-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140776598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can irisin be developed as the molecular evolutionary clock based on the origin and functions?","authors":"Junyu Chen ,&nbsp;Lijun Guo ,&nbsp;Chenglong Wang ,&nbsp;Peng Peng ,&nbsp;Jiaming Wu ,&nbsp;Huaidong Zhang ,&nbsp;Feng Liu ,&nbsp;Qin Li","doi":"10.1016/j.ygcen.2024.114515","DOIUrl":"https://doi.org/10.1016/j.ygcen.2024.114515","url":null,"abstract":"<div><p>Irisin, a myokine identified in 2012, has garnered research interest for its capacity to induce browning of adipocytes and improve metabolic parameters. As such, the potential therapeutic applications of this exercise-induced peptide continue to be explored. Though present across diverse animal species, sequence analysis has revealed subtle variation in the irisin protein. In this review, we consider the effects of irisin on disease states in light of its molecular evolution. We summarize current evidence for irisin's influence on pathologies and discuss how sequence changes may inform development of irisin-based therapies. Furthermore, we propose that the phylogenetic variations in irisin could potentially be leveraged as a molecular clock to elucidate evolutionary relationships.</p></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140533811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormonal effects on glucose and ketone metabolism in a perfused liver of an elasmobranch, the North Pacific spiny dogfish, Squalus suckleyi","authors":"Alexandra N. Schoen ,&nbsp;Alyssa M. Weinrauch ,&nbsp;Ian A. Bouyoucos ,&nbsp;Jason R. Treberg ,&nbsp;W. Gary Anderson","doi":"10.1016/j.ygcen.2024.114514","DOIUrl":"https://doi.org/10.1016/j.ygcen.2024.114514","url":null,"abstract":"<div><p>Hormonal influence on hepatic function is a critical aspect of whole-body energy balance in vertebrates. Catecholamines and corticosteroids both influence hepatic energy balance via metabolite mobilization through glycogenolysis and gluconeogenesis. Elasmobranchs have a metabolic organization that appears to prioritize the mobilization of hepatic lipid as ketone bodies (e.g. 3-hydroxybutyrate [3-HB]), which adds complexity in determining the hormonal impact on hepatic energy balance in this taxon. Here, a liver perfusion was used to investigate catecholamine (epinephrine [E]) and corticosteroid (corticosterone [B] and 11-deoxycorticosterone [DOC]) effects on the regulation of hepatic glucose and 3-HB balance in the North Pacific Spiny dogfish, <em>Squalus suckleyi</em>. Further, hepatic enzyme activity involved in ketogenesis (3-hydroxybutyrate dehydrogenase), glycogenolysis (glycogen phosphorylase), and gluconeogenesis (phosphoenolpyruvate carboxykinase) were assessed in perfused liver tissue following hormonal application to discern effects on hepatic energy flux. mRNA transcript abundance key transporters of glucose (<em>glut1</em> and <em>glut4</em>) and ketones (<em>mct1</em> and <em>mct2</em>) and glucocorticoid function (<em>gr</em>, <em>pepck</em>, <em>fkbp5</em>, and <em>11βhsd2</em>) were also measured to investigate putative cellular components involved in hepatic responses. There were no changes in the arterial-venous difference of either metabolite in all hormone perfusions. However, perfusion with DOC increased <em>gr</em> transcript abundance and decreased flow rate of perfusions, suggesting a regulatory role for this corticosteroid. Phosphoenolpyruvate carboxykinase activity increased following all hormone treatments, which may suggest gluconeogenic function; E also increased 3-hydroxybutyrate dehydrogenase activity, suggesting a function in ketogenesis, and decreased <em>pepck</em> and <em>fkbp5</em> transcript abundance, potentially showing some metabolic regulation. Overall, we demonstrate hormonal control of hepatic energy balance using liver perfusions at various levels of biological organization in an elasmobranch.</p></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140533810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The expression profiles of cyp19a1, sf-1, esrs and gths in the brain-pituitary during gonadal sex differentiation in juvenile Japanese eels","authors":"Shan-Ru Jeng ,&nbsp;Guan-Chung Wu ,&nbsp;Wen-Shiun Yueh ,&nbsp;Pei-hua Liu ,&nbsp;Shu-Fen Kuo ,&nbsp;Sylvie Dufour ,&nbsp;Ching-Fong Chang","doi":"10.1016/j.ygcen.2024.114512","DOIUrl":"https://doi.org/10.1016/j.ygcen.2024.114512","url":null,"abstract":"<div><p>Eels are gonochoristic species whose gonadal differentiation initiates at the yellow eel stage and is influenced by environmental factors. We revealed some sex-related genes were sex dimorphically expressed in gonads during gonadal sex differentiation of Japanese eel (<em>Anguilla japonica</em>); however, the expression of sex-related genes in the brain-pituitary during gonadal sex differentiation in eels is still unclear. This study aimed to investigate the sex-related gene expressions in the brain-pituitary and tried to clarify their roles in the brain and gonads during gonadal sex differentiation. Based on our previous histological study, the control eels developed as males, and estradiol-17β (E2) was used for feminization. Our results showed that during testicular differentiation, the brain <em>cyp19a1</em> transcripts and aromatase proteins were increased significantly; moreover, the <em>cyp19a1</em>, <em>sf-1</em>, <em>foxl2s</em>, and <em>esrs</em> (except <em>gperb</em>) transcripts in the midbrain/pituitary also were increased significantly. Forebrain <em>gnrh1</em> transcripts increased slightly during gonadal differentiation of both sexes, but the <em>gnrhr1b</em> and <em>gnrhr2</em> transcripts in the midbrain/pituitary were stable during gonadal differentiation. The expression levels of <em>gths</em> and <em>gh</em> in the midbrain/pituitary were significantly increased during testicular differentiation and were much higher in males than in E2-feminized females. These results implied that endogenous estrogens might play essential roles in the brain/pituitary during testicular differentiation, <em>sf-1</em>, <em>foxl2s</em>, and <em>esrs</em> may have roles in <em>cyp19a1</em> regulation in the midbrain/pituitary of Japanese eels. For the GnRH-GTH axis, <em>gths</em>, especially <em>fshb</em>, may be regulated by <em>esrs</em> and involved in regulating testicular differentiation and development in Japanese eels.</p></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140554008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual role of pregnancy in breast cancer risk","authors":"Shiting Fu ,&nbsp;Hao Ke ,&nbsp;Huozhong Yuan ,&nbsp;Huaimeng Xu ,&nbsp;Wenyan Chen ,&nbsp;Limin Zhao","doi":"10.1016/j.ygcen.2024.114501","DOIUrl":"10.1016/j.ygcen.2024.114501","url":null,"abstract":"<div><p>Reproductive history is one of the strongest risk factors for breast cancer in women. Pregnancy can promote short-term breast cancer risk, but also reduce a woman’s lifetime risk of breast cancer. Changes in hormone levels before and after pregnancy are one of the key factors in breast cancer risk. This article summarizes the changes in hormone levels before and after pregnancy, and the roles of hormones in mammary gland development and breast cancer progression. Other factors, such as changes in breast morphology and mammary gland differentiation, changes in the proportion of mammary stem cells (MaSCs), changes in the immune and inflammatory environment, and changes in lactation before and after pregnancy, also play key roles in the occurrence and development of breast cancer. This review discusses the dual effects and the potential mechanisms of pregnancy on breast cancer risk from the above aspects, which is helpful to understand the complexity of female breast cancer occurrence.</p></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140287314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional analysis of circpdlim5a generated from pdlim5a gene splicing in the skeletal muscle of Japanese flounder (Paralichthys olivaceus)","authors":"Hao Wang,&nbsp;Min Liu,&nbsp;Hengtai Tang,&nbsp;Zhirui Zhang,&nbsp;Haishen Wen,&nbsp;Feng He","doi":"10.1016/j.ygcen.2024.114500","DOIUrl":"10.1016/j.ygcen.2024.114500","url":null,"abstract":"<div><p>Circular RNAs (circRNAs) are non-coding RNAs with endogenous regulatory functions, including regulating skeletal muscle development. However, its role in the development of skeletal muscle in Japanese flounder (<em>Paralichthys olivaceus</em>) is not clear. Therefore we screened a candidate circpdlim5a, which is derived from the gene <em>pdlim5a</em>, from the skeletal muscle transcriptome of Japanese flounder. We characterized circpdlim5a, which was more stable compared to the linear RNA <em>pdlim5a</em>. Distributional characterization of circpdlim5a showed that circpdlim5a was predominantly distributed in the nucleus and was highly expressed in the skeletal muscle of adult Japanese flounder (24 months). When we further studied the circpdlim5a function, we found that it inhibited the expression of proliferation and differentiation genes according to the over-expression experiment of circpdlim5a in myoblasts. We concluded that circpdlim5a may inhibit the proliferation and differentiation of myoblasts and thereby inhibit skeletal muscle development in Japanese flounder. This experiment provides information for the study of circRNAs by identifying circpdlim5a and exploring its function, and offers clues for molecular breeding from an epigenetic perspective.</p></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140151747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural modeling and gene expression analysis of phosvitinless vitellogenin (vgc) in the Indian freshwater murrel, Channa punctatus (Bloch, 1793)","authors":"Pooja Vijay ,&nbsp;Deepak Panwar ,&nbsp;Ritu Narwal ,&nbsp;Neeta Sehgal","doi":"10.1016/j.ygcen.2024.114491","DOIUrl":"10.1016/j.ygcen.2024.114491","url":null,"abstract":"<div><p>Vitellogenin (Vg) is a female-specific egg-yolk precursor protein, synthesized in the liver of fish in response to estrogens. In the present study, complete gene of phosvitinless vitellogenin (<em>vgc)</em> was sequenced, its 3D structure was predicted and validated by web-based softwares. The complete nucleotide sequence of <em>vgc</em> was 4126 bp which encodes for 1272 amino acids and showed the presence of three conserved domains viz. LPD_N, DUF1943 and DUF1944. The retrieved amino acid sequence of VgC protein was subjected to <em>in silico</em> analysis for understanding the structural and functional properties of protein. mRNA levels of multiple <em>vg</em> genes have also been quantified during annual reproductive cycle employing qPCR. A correlation has been observed between seasonal changes in gonadosomatic index with estradiol levels and hepatic expression of three types of <em>vg</em> genes (<em>vga, vgb, vgc</em>) during ovarian cycle of murrel. During preparatory phase, when photoperiod and temperature are low; low titre of E₂ in blood induces expression of <em>vgc</em> gene. A rapid increase in the levels of E₂ favours induction of <em>vgb</em> and <em>vga</em> genes in liver of murrel during early pre-spawning phase when photoperiod is long and temperature is high in nature. These results suggest that among three vitellogenin proteins, VgC is synthesized earlier than VgA and VgB during oogenesis.</p></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140143097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A longitudinal study of endocrinology and foraging ecology of subadult gray whales prior to death based on baleen analysis","authors":"Alejandro Fernández Ajó ,&nbsp;Clarissa Teixeira ,&nbsp;Daniela M.D. de Mello ,&nbsp;Danielle Dillon ,&nbsp;James M. Rice ,&nbsp;C. Loren Buck ,&nbsp;Kathleen E. Hunt ,&nbsp;Matthew C. Rogers ,&nbsp;Leigh G. Torres","doi":"10.1016/j.ygcen.2024.114492","DOIUrl":"10.1016/j.ygcen.2024.114492","url":null,"abstract":"<div><p>Individual-level assessments of wild animal health, vital rates, and foraging ecology are critical for understanding population-wide impacts of exposure to stressors. Large whales face multiple stressors, including, but not limited to, ocean noise, pollution, and ship strikes. Because baleen is a continuously growing keratinized structure, serial extraction, and quantification of hormones and stable isotopes along the length of baleen provide a historical record of whale physiology and foraging ecology. Furthermore, baleen analysis enables the investigation of dead specimens, even decades later, allowing comparisons between historic and modern populations. Here, we examined baleen of five sub-adult gray whales and observed distinct patterns of oscillations in <em>δ</em>¹⁵N values along the length of their baleen plates which enabled estimation of baleen growth rates and differentiation of isotopic niche widths of the whales during wintering and summer foraging. In contrast, no regular patterns were apparent in <em>δ</em>¹³C values. Prolonged elevation of cortisol in four individuals before death indicates that chronic stress may have impacted their health and survival. Triiodothyronine (T3) increased over months in the whales with unknown causes of death, simultaneous with elevations in cortisol, but both hormones remained stable in the one case of acute death attributed to killer whale predation. This parallel elevation of cortisol and T3 challenges the classic understanding of their interaction and might relate to increased energetic demands during exposure to stressors. Reproductive hormone profiles in subadults did not show cyclical trends, suggesting they had not yet reached sexual maturity. This study highlights the potential of baleen analysis to retrospectively assess gray whales' physiological status, exposure to stressors, reproductive status, and foraging ecology in the months or years leading up to their death, which can be a useful tool for conservation diagnostics to mitigate unusual mortality events.</p></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early life nutrient restriction affects hypothalamic-pituitary-interrenal axis gene expression in a diet type-specific manner","authors":"Alexander M. Shephard ,&nbsp;Sarah R. Lagon ,&nbsp;Cristina C. Ledón-Rettig","doi":"10.1016/j.ygcen.2024.114490","DOIUrl":"10.1016/j.ygcen.2024.114490","url":null,"abstract":"<div><p>Stressful experiences in early life can alter phenotypic expression later in life. For instance, in vertebrates, early life nutrient restriction can modify later life activity of the hypothalamic–pituitary–adrenal/interrenal axis (the HPI in amphibians), including the up- and downstream regulatory components of glucocorticoid signaling. Early life nutrient restriction can also influence later life behavior and metabolism (<em>e.g.</em>, fat accumulation). Yet, less is known about whether nutrient stress-induced carryover effects on HPA/HPI axis regulation can vary across environmental contexts, such as the type of diet on which nutrient restriction occurs. Here, we experimentally address this question using the plains spadefoot toad (<em>Spea bombifrons</em>), whose larvae develop in ephemeral habitats that impose intense competition over access to two qualitatively distinct diet types: detritus and live shrimp prey. Consistent with diet type-specific carryover effects of early life nutrient restriction on later life HPI axis regulation, we found that temporary nutrient restriction at the larval stage reduced juvenile (<em>i.e.,</em> post-metamorphic) brain gene expression of an upstream glucocorticoid regulator (corticotropin-releasing hormone) and two downstream regulators (glucocorticoid and mineralocorticoid receptors) only on the shrimp diet. These patterns are consistent with known diet type-specific effects of larval nutrient restriction on juvenile corticosterone and behavior. Additionally, larval nutrient restriction increased juvenile body fat levels. Our study indicates that HPA/HPI axis regulatory responses to nutrient restriction can vary remarkably across diet types. Such diet type-specific regulation of the HPA/HPI axis might provide a basis for developmental or evolutionary decoupling of stress-induced carryover effects.</p></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140068324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}