General and comparative endocrinology最新文献_第8页

Identification and expression analysis of three gonadotropin-releasing hormone genes in the lined seahorse (Hippocampus erectus) 三种促性腺激素释放激素基因在海马体中的鉴定与表达分析。

IF 2.1 3区医学

General and comparative endocrinology Pub Date : 2025-02-01 DOI: 10.1016/j.ygcen.2025.114673

Zijian Lu , Shiming Wan , Qiang Lin , Huixian Zhang

{"title":"Identification and expression analysis of three gonadotropin-releasing hormone genes in the lined seahorse (Hippocampus erectus)","authors":"Zijian Lu , Shiming Wan , Qiang Lin , Huixian Zhang","doi":"10.1016/j.ygcen.2025.114673","DOIUrl":"10.1016/j.ygcen.2025.114673","url":null,"abstract":"<div><div>Gonadotropin-releasing hormone (GnRH) plays a crucial role in regulating reproductive behavior in vertebrates through the hypothalamus-pituitary-gonad (HPG) axis. Seahorses exhibit unique male pregnancy behavior, making them an interesting subject for investigating the regulatory mechanisms behind this behavior. In this study, we focused on the lined seahorse (<em>Hippocampus erectus</em>) and obtained the full-length cDNA sequences of three <em>GnRH</em> genes: <em>GnRH1</em>, <em>GnRH2</em>, and <em>GnRH3</em>. Notably, we newly identified <em>GnRH1</em>, filling a gap in previous research that had overlooked this subtype. Through a homologous analysis, we found that the core peptide of GnRH1 in lined seahorses is not common in teleosts, while the core peptides of GnRH2 and GnRH3 are conserved. Real-time PCR was performed to determine the tissue expression patterns of these <em>GnRH</em> genes. Our results showed that all three genes were predominantly expressed in the brain, albeit in different regions. Specifically, <em>GnRH1</em> was mainly expressed in the hypothalamus, <em>GnRH2</em> in the optic tectum, and <em>GnRH3</em> in the telencephalon. Expression dynamics indicated a progressive decrease in <em>GnRH2</em> and a significant surge in <em>GnRH3</em> during gonadal development, hinting at an alternating regulatory function. Moreover, <em>GnRH1</em> and <em>GnRH3</em> expression levels were considerably higher during pregnancy compared to those in pre-pregnancy and post-pregnancy stages, underscoring their critical role in modulating male pregnancy behavior in lined seahorses. Our findings provide insights into the complex interplay of GnRH subtypes in regulating reproductive processes, particularly in the context of male pregnancy behavior in seahorses.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"362 ","pages":"Article 114673"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The role of the opioid peptide dynorphin during the seasonal and gonadotropin-induced ovarian recrudescence in the gecko 阿片肽达诺啡在壁虎季节性和促性腺激素诱导的卵巢复旧过程中的作用

IF 2.1 3区医学

General and comparative endocrinology Pub Date : 2025-02-01 DOI: 10.1016/j.ygcen.2025.114684

Ananya Ganeyan, C.B. Ganesh

{"title":"The role of the opioid peptide dynorphin during the seasonal and gonadotropin-induced ovarian recrudescence in the gecko","authors":"Ananya Ganeyan, C.B. Ganesh","doi":"10.1016/j.ygcen.2025.114684","DOIUrl":"10.1016/j.ygcen.2025.114684","url":null,"abstract":"<div><div>The aim of the current investigation was to elucidate the influence of the opioid peptide dynorphin-A (DYN) on the reproductive axis during breeding and non-breeding phases of the ovarian cycle in the gecko <em>Hemidactylus frenatus.</em> During the recrudescence phase, administration of a high dose of DYN (10 µg/0.1 ml saline) caused a significant reduction in the numbers of oogonia and primary oocytes in the germinal bed, compared to those of the initial controls or experimental controls. Administration of a low (2 µg DYN/0.1 ml saline) or high dose of DYN did not affect the follicular development up to stage IV, but there were no stage V (vitellogenic) follicles in the ovary in contrast to their presence in the experimental controls. Furthermore, there was a significant reduction in gonadotropin-releasing hormone-immunoreactive (GnRH-ir) content in the median eminence (ME) and pars distalis of the pituitary gland (PD) in low or high doses of DYN-treated lizards. During the regression phase, treatment with follicle-stimulating hormone (FSH) resulted in the appearance of stage IV and V follicles, in contrast to their absence in initial controls and treatment controls. However, treatment with 10 µg DYN + FSH did not promote the development of these follicles. In addition, <em>in vitro</em> treatment of DYN significantly inhibited ovarian levels of estradiol. Collectively, these findings reveal an inhibitory influence of DYN on the seasonal ovarian recrudescence, possibly mediated through the suppression of GnRH release into the ME and PD and directly at the level of the ovary by impairment in steroidogenesis and vitellogenic follicular growth in the gecko.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"363 ","pages":"Article 114684"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Neuromodulation in the fish brain for reproductive success 鱼类大脑中对繁殖成功的神经调节。

IF 2.1 3区医学

General and comparative endocrinology Pub Date : 2025-02-01 DOI: 10.1016/j.ygcen.2024.114658

Chie Umatani

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The S6 kinase gene in the fruit fly, Drosophila melanogaster, is essential for metabolic regulation 果蝇（Drosophila melanogaster）体内的S6激酶基因对代谢调节至关重要。

IF 2.1 3区医学

General and comparative endocrinology Pub Date : 2025-02-01 DOI: 10.1016/j.ygcen.2025.114672

Jéssica Paloma Álvarez-Rendón , Juan Rafael Riesgo-Escovar

{"title":"The S6 kinase gene in the fruit fly, Drosophila melanogaster, is essential for metabolic regulation","authors":"Jéssica Paloma Álvarez-Rendón , Juan Rafael Riesgo-Escovar","doi":"10.1016/j.ygcen.2025.114672","DOIUrl":"10.1016/j.ygcen.2025.114672","url":null,"abstract":"<div><div>The S6 kinase (S6K) enzyme phosphorylates the S6 ribosomal protein, promoting protein translation and growth. Here we investigated in flies whether hypomorphic conditions in S6K affect intermediate metabolism and oxidative homeostasis, besides carbohydrates and growth. We also employed partial activation of the nuclear factor 2 erythroid related factor 2 (Nrf2) in a S6K hypomorphic background and controls. S6K is activated by the target of rapamycin (TOR) kinase, a key kinase regulating metabolism, downstream of the insulin receptor in flies. The insulin pathway is a general anabolic pathway, and key regulator of glucose homeostasis. The Nrf2 counters pro-oxidative conditions, also involved in inflammatory responses and metabolism. The Nrf2 fly homolog is Cap’n’collar C (CncC). We quantified glucose, glycogen, and total lipids in control and different pro-oxidative conditions. We corroborated an accumulation of lipids and carbohydrates in the mutants, and document sexual differences. We document also metabolic and survival differences in the responses to “mild” pro-oxidative conditions in young flies (seven days old), with females being most affected. We compare 10 mM paraquat survival of virgin flies to mated mixed-sex flies housed together. We used females to study transcriptomic differences between wild type and <em>S6k</em> hypomorphs. Results highlight dysregulation of lipid and antioxidant enzymes and genes, in agreement with lipid and oxidative metabolism data. Our results are consistent with the insulin/TOR pathway acting as an integrator of intermediate metabolism and oxidative homeostasis (this last together with the CncC pathway).</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"362 ","pages":"Article 114672"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Production of recombinant masu salmon insulin-like growth factor binding protein-2b1 and its action on pituitary cells 重组马苏鲑鱼胰岛素样生长因子结合蛋白2b1的制备及其对垂体细胞的作用。

IF 2.1 3区医学

General and comparative endocrinology Pub Date : 2025-02-01 DOI: 10.1016/j.ygcen.2025.114674

Takuto Miura , Sota Watanabe , Ryousuke Kizaki , Ryuya Hasegawa , Taiga Isozaki , Munetaka Shimizu

{"title":"Production of recombinant masu salmon insulin-like growth factor binding protein-2b1 and its action on pituitary cells","authors":"Takuto Miura , Sota Watanabe , Ryousuke Kizaki , Ryuya Hasegawa , Taiga Isozaki , Munetaka Shimizu","doi":"10.1016/j.ygcen.2025.114674","DOIUrl":"10.1016/j.ygcen.2025.114674","url":null,"abstract":"<div><div>Insulin-like growth factor binding protein (Igfbp)-2b is believed to be a major carrier of circulating Igf-1 in salmonids. We cloned cDNAs of two paralogs of <em>igfbp-2b</em> from the liver of masu salmon and produced recombinant Igfbp-2b1 corresponding to the circulating form using a bacterial expression system. The deduced amino acid sequence of masu salmon <em>igfbp-2b1</em> had a 75.2 % sequence identity with that of masu salmon <em>igfbp-2b2</em>, and 88.7 % and 96.5 % with those of Atlantic salmon and rainbow trout <em>igfbp-2b1</em>, respectively. The coding region of masu salmon <em>igfbp-2b1</em> cDNA was subcloned into the pET-16b or pET-32a vector and expressed using either a histidine (His)-tag or a thioredoxin (Trx) and His-tag. Recombinant masu salmon (rs) Igfbp-2b1 with the fusion partner was fractionated in the precipitate, solubilized, and isolated using Ni-affinity chromatography. His.rsIgfbp-2b1 and Trx.His.rsIgfbp-2b1 were treated with Factor Xa and enterokinase K, respectively, to remove the fusion partner; only the digestion with enterokinase was successful. After enzymatic digestion, rsIgfbp-2b1 was purified employing reversed-phase high-performance liquid chromatography. The purified rsIgfbp-2b1 was added to a primary culture of masu salmon pituitary cells with or without human (h) IGF-1 to assess its effect on the release of growth hormone (Gh). Although addition of hIGF-1 alone had no effect on Gh release, co-incubation with varying amounts of rsIgfbp-2b1 increased Gh release in a dose-dependent manner. In addition, rsIgfbp-2b1 in the absence of hIGF-1 showed a positive effect on Gh release from salmon pituitary cells. These results suggest that rsIgfbp-2b1 may either have Igf-1–independent action on Gh release or inhibits the suppressive effect of local pituitary Igf-1 on Gh release.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"363 ","pages":"Article 114674"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Influence of season, capture method, sample age and extraction protocols on the scale cortisol concentrations of three species of freshwater fish 季节、捕获方法、样品年龄和提取方案对三种淡水鱼鳞皮质醇浓度的影响。

IF 2.1 3区医学

General and comparative endocrinology Pub Date : 2025-02-01 DOI: 10.1016/j.ygcen.2025.114671

Ahmad Ghazal , Richard Paul , A. Serhan Tarkan , J. Robert Britton

{"title":"Influence of season, capture method, sample age and extraction protocols on the scale cortisol concentrations of three species of freshwater fish","authors":"Ahmad Ghazal , Richard Paul , A. Serhan Tarkan , J. Robert Britton","doi":"10.1016/j.ygcen.2025.114671","DOIUrl":"10.1016/j.ygcen.2025.114671","url":null,"abstract":"<div><div>Scale cortisol concentration (SCC) is increasingly applied as a biomarker of chronic stress in fish, but knowledge gaps remain on how SCC is affected by the sampling season and method of fish capture, the time since sample collection, and the cortisol extraction protocol. Here, working with three freshwater fishes (common carp <em>Cyprinus carpio</em>, European chub <em>Squalius cephalus</em> and Northern pike <em>Esox lucius</em>), a robust extraction protocol was developed and then applied to identifying how scale cortisol levels can vary in fish populations according to aspects of the fish capture events. Across five scale cortisol extraction protocols, three provided relatively low yields, so their application would result in erroneously low SCC. Application of the extraction protocol providing the highest yields to scale samples indicated that fish sampled in winter have significantly lower SCC than those collected in spring and summer, while fish captured by angling have significantly lower SCC than fish collected from the same population by electric fishing. There were no significant differences in SCC measured from populations across 40 years, suggesting that archived scales potentially provide a valuable resource for measuring temporal changes in SCC. Future studies based on using scale cortisol for analyses of fish chronic stress should consider these issues in their study designs and evaluations to ensure measured differences in cortisol across time and space are due to differences in how the fish are responding to their environment rather than being an artefact of study design.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"362 ","pages":"Article 114671"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Enhanced expression of Cyp17a1 and production of DHEA-S in the liver of late-pregnant rats 妊娠晚期大鼠肝脏中Cyp17a1的表达和DHEA-S的产生增加。

IF 2.1 3区医学

General and comparative endocrinology Pub Date : 2025-02-01 DOI: 10.1016/j.ygcen.2025.114661

Yuya Ohtsuki , Jumpei Fujiki , Naoyuki Maeda , Hidetomo Iwano

{"title":"Enhanced expression of Cyp17a1 and production of DHEA-S in the liver of late-pregnant rats","authors":"Yuya Ohtsuki , Jumpei Fujiki , Naoyuki Maeda , Hidetomo Iwano","doi":"10.1016/j.ygcen.2025.114661","DOIUrl":"10.1016/j.ygcen.2025.114661","url":null,"abstract":"<div><div>Cytochrome P450 17A1 (CYP17A1) catalyzes two enzymatic reactions in the biosynthesis of dehydroepiandrosterone (DHEA) from pregnenolone. In pregnant humans, the adrenal gland is responsible for DHEA biosynthesis, which is then sulfated by SULT2A1 and released into the bloodstream. This sulfated DHEA is subsequently taken up by the placenta and deconjugated to serve as a precursor for estrogen biosynthesis. The expression of <em>Cyp17a1</em> is regulated by methylation, typically showing marked interspecies differences, including repression of <em>Cyp17a1</em> expression in the adrenal gland of rodents. This study focused on the liver, an extragonadal steroidogenic organ showing active sulfate conjugation, as a site for DHEA-sulfate (DHEA-S) biosynthesis during pregnancy in rodents, rather than the adrenal glands. <em>Cyp17a1</em> expression in rat liver was significantly lower than in the testis, with no differences between sexes. However, <em>Cyp17a1</em> expression increased significantly before parturition (gestational days [GD] 19–21) compared to late pregnancy (GD 15–18). The <em>Sult2a</em> family were expressed in the livers of both pregnant and non-pregnant rats. We also observed increased DHEA and DHEA-S levels in the liver of pregnant rats before parturition compared to non-pregnant rats, with DHEA-S concentrations being significantly higher at GD 19–21 than at days 15–18. These findings suggest that increased expression of <em>Cyp17a1</em> in the last trimester enhances DHEA synthesis in the liver, and that DHEA is quickly conjugated by Sult2a. In rodents, the liver may be involved in DHEA-S biosynthesis before parturition, compensating for the repression of <em>Cyp17a1</em> in the adrenal glands.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"362 ","pages":"Article 114661"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Hormonology: A kaleidoscopic tale of dedication, serendipity, and ingenuity 荷尔蒙：一个千变万化的故事，奉献，意外发现，和独创性

IF 2.1 3区医学

General and comparative endocrinology Pub Date : 2025-02-01 DOI: 10.1016/j.ygcen.2025.114676

Aaron J.W. Hsueh , Jianzhen Li

{"title":"Hormonology: A kaleidoscopic tale of dedication, serendipity, and ingenuity","authors":"Aaron J.W. Hsueh , Jianzhen Li","doi":"10.1016/j.ygcen.2025.114676","DOIUrl":"10.1016/j.ygcen.2025.114676","url":null,"abstract":"<div><div>This article delves into stories of hormone discovery, characterization, clinical application, and the scientists themselves. Our journey begins with Arnold Berthod’s rooster castration experiments, laying the groundwork for understanding testosterone, and Ernest Starling’s 1902 revelation that secretin wasn’t neuron-released. We’ll explore the origins of kisspeptin and its role in Gonadotropin-releasing hormone pulsatile secretion by KNDY neurons. Discussions will then include the isolation of Growth hormone-releasing hormone from pancreatic tumors and growth hormone therapy’s surprising link to Creutzfeldt-Jakob disease. We’ll also delve into the design of long-acting follicle stimulating hormone for infertility treatment, alongside discoveries of five inhibin and activin hormones. Further, we’ll uncover the discovery of receptors for relaxin family ligands and diverse chemokines, highlighting scientists’ use of evolutionary genomics. We’ll touch on parabiotic animal experiments leading to leptin’s discovery and polarized ligand-receptor expression for receptor activation. The narrative will include instances such as a Nobel laureate who presented a rejection letter received prior to their award ceremony and a researcher pursuing receptor structure despite funding setbacks, both eventually rewarded. Through the diligent efforts of researchers and unexpected discoveries, the mechanisms of more than a hundred hormones have been elucidated. The endocrinological research domain is currently embracing the application of artificial intelligence to analyze extensive genomic and protein structure databases.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"363 ","pages":"Article 114676"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143386400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A newly characterized CFSH gene in sex chromosomes is associated with growth instead of sexual development in the prawn Macrobrachium rosenbergii 罗氏沼虾（Macrobrachium rosenbergii）性染色体上新发现的CFSH基因与生长而非性发育有关。

IF 2.1 3区医学

General and comparative endocrinology Pub Date : 2025-01-01 DOI: 10.1016/j.ygcen.2024.114654

Xing-Lin Lai, Zhen-Hua Zhang, Jia-Yan Chen, Yu-Ru Liu, Wei-Kun Yun, Li-Ping Chen, Jie Chen, Gao-Feng Qiu

{"title":"A newly characterized CFSH gene in sex chromosomes is associated with growth instead of sexual development in the prawn Macrobrachium rosenbergii","authors":"Xing-Lin Lai, Zhen-Hua Zhang, Jia-Yan Chen, Yu-Ru Liu, Wei-Kun Yun, Li-Ping Chen, Jie Chen, Gao-Feng Qiu","doi":"10.1016/j.ygcen.2024.114654","DOIUrl":"10.1016/j.ygcen.2024.114654","url":null,"abstract":"<div><div>Crustacean female sex hormone (<em>CFSH</em>) was first identified as a female eyestalk-specific factor involved in the female sexual development in blue crab. Whether <em>CFSH</em> has conserved role in other decapod species remains to be clarified. In this study, we identified a <em>CFSH</em> gene (<em>MrCFSH</em>) in the Z and W chromosomes from the prawn <em>Macrobrachium rosenbergii</em> genome. The <em>MrCFSH</em> encodes a protein of 306 amino acids, including a signal peptide, a precursor-associated peptide, and a mature peptide. The <em>MrCFSH</em> transcripts was expressed in the brain, heart, and gonads in both female and male adults. During sexual development, the expression of <em>MrCFSH</em> transcripts gradually increased in female and male juveniles from post-larval 10 (PL10) to PL60 stages but no significant difference between the females and males at the same stages were found. To investigate its potential role in early sexual development, in vivo RNAi experiments were performed by injecting <em>MrCFSH</em> dsRNA into juveniles from PL30 to PL90 stages. After two months of administration, the sexual characteristics of the females and males were not affected, but the growth rate of juveniles was significantly inhibited compared to the control group injected with <em>GFP</em> dsRNA. The body length and body weight was reduced by 25%, 35%, 28%, 26%, and 69%, 76%, 65%, 61% in the juveniles at PL120, PL150, PL180 and PL210 stages, respectively. The body length and weight of males at PL210 stage were reduced by 22% and 54%, and those of females were reduced by 26% and 60%, respectively. Overall, our present study revealed that the <em>MrCFSH</em> is associated with growth instead of sexual development in the prawn <em>M. rosenbergii</em>.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"361 ","pages":"Article 114654"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corticosterone, lactate, and circulating leukocyte responses of free-ranging cottonmouth snakes (Agkistrodon piscivorus) vary with the duration and nature of the acute stressor 自由放养的水嘴蛇（Agkistrodon piscivorus）的皮质酮、乳酸和循环白细胞反应随急性应激源的持续时间和性质而变化。

IF 2.1 3区医学

General and comparative endocrinology Pub Date : 2025-01-01 DOI: 10.1016/j.ygcen.2024.114660

Matthew Klukowski , Frank C. Bailey , Vincent A. Cobb , Jake A. Pruett

{"title":"Corticosterone, lactate, and circulating leukocyte responses of free-ranging cottonmouth snakes (Agkistrodon piscivorus) vary with the duration and nature of the acute stressor","authors":"Matthew Klukowski , Frank C. Bailey , Vincent A. Cobb , Jake A. Pruett","doi":"10.1016/j.ygcen.2024.114660","DOIUrl":"10.1016/j.ygcen.2024.114660","url":null,"abstract":"<div><div>Exposure to acute stressors can induce multiple physiological changes in vertebrates such as altering circulating hormone and enzyme levels as well as leukocyte counts, and interactions between endocrine and immune function may produce suites of physiological changes following acute stress. Previously, we showed that presence of human observers elicited only a weak elevation of plasma corticosterone levels in cottonmouths. Additional variables, however, must be considered to understand if changes in physiological parameters are highly generalized or vary among sexes or with context. Here we tested effects of more intense acute stressors (i.e., confinement for 2 h or 4 h or repeated blood sampling) on plasma corticosterone and lactate concentrations and the number of circulating leukocytes. In addition to testing for sex differences, we also tested two predictions frequently found in the stress physiology literature: (1) because glucocorticoids are involved in mobilization of leukocytes, corticosterone levels should covary with the heterophil to lymphocyte ratio, (2) since one of the major functions of corticosterone is to mobilize energy stores, baseline corticosterone levels (i.e., in immediately bled snakes) should be elevated in individuals in poor body condition. We found that neither sex nor body condition influenced corticosterone responses to confinement. However, repeated blood sampling had a pronounced effect– snakes bled both upon capture and at 2 h had higher corticosterone levels than snakes bled only once at 2 h or even at 4 h. Plasma lactate and the percent azurophils (but not the heterophil to lymphocyte ratio) were also elevated in confined snakes, and both were positively correlated with plasma corticosterone. Our results indicate a pronounced and nuanced effect of confinement stress on the physiology of cottonmouths.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"361 ","pages":"Article 114660"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0