Identification and expression of nuclear receptor genes during nutritive phagocyte development in sea urchin Mesocentrotus nudus gonads

IF 1.7 3区医学 Q3 ENDOCRINOLOGY & METABOLISM

General and comparative endocrinology Pub Date : 2025-03-24 DOI:10.1016/j.ygcen.2025.114715

Kazuhiro Ura , Shiori Tsue , Takushi Sato , Seishi Hagihara , Mizuha Satoh , Ichiro Higuchi , Heng Wang , Osamu Nishimiya , Shigeho Ijiri , Yasuaki Takagi

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引用次数: 0

Abstract

The role of the endocrine system during synthesis of the nutrients (e.g., proteins, lipids, polysaccharides) in the gonad in sea urchins is imperfectly understood. We identify genes encoding nuclear receptors (NRs) involved in the development of nutritive phagocytes of the sea urchin Mesocentrotus nudus, and investigate their gene expression patterns during this time. RNA-seq analysis was performed on immature gonads using a next-generation sequencer. A total of 7,651,421 quality-controlled reads obtained using an Ion PGM sequencer were assembled into 175,092 contigs. BLASTn analysis identified 20 NR genes potentially involved in the development of nutritive phagocytes. Expression analyses revealed levels of four NR genes to increase during the development of nutritive phagocytes. These results indicate that these 20 NR genes have physiological functions in gonadal growth, and that 4 NR genes mainly control the expression of genes involved in the synthesis and metabolism of proteins, lipids, and polysaccharides during cellular proliferation in sea urchin gonads.

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海胆生殖腺营养吞噬细胞发育过程中核受体基因的鉴定与表达

在海胆的生殖腺中，内分泌系统在营养物质（如蛋白质、脂质、多糖）合成过程中的作用尚不完全清楚。我们鉴定了参与海胆（mesocentrrotus nudus）营养吞噬细胞发育的核受体（NRs）编码基因，并研究了它们在此期间的基因表达模式。使用下一代测序仪对未成熟性腺进行RNA-seq分析。使用Ion PGM测序仪获得的总共7,651,421个质量控制reads被组装成175,092个contigs。BLASTn分析鉴定出20个NR基因可能参与营养性吞噬细胞的发育。表达分析显示，在营养性吞噬细胞发育过程中，4种NR基因的表达水平升高。上述结果表明，这20个NR基因在海胆性腺生长过程中具有生理功能，其中4个NR基因主要控制海胆性腺细胞增殖过程中蛋白质、脂质和多糖合成代谢相关基因的表达。

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来源期刊

General and comparative endocrinology 医学-内分泌学与代谢

CiteScore

5.60

自引率

7.40%

发文量

120

审稿时长

2 months

期刊介绍： General and Comparative Endocrinology publishes articles concerned with the many complexities of vertebrate and invertebrate endocrine systems at the sub-molecular, molecular, cellular and organismal levels of analysis.