Gels最新文献_第4页

Utilisation of High Molecular Weight and Ultra-High Molecular Weight Hyaluronan in Management of Glioblastoma.

IF 5 3区化学

Gels Pub Date : 2025-01-08 DOI: 10.3390/gels11010050

Alex-Adrian Salagean, Cezara-Anca-Denisa Moldovan, Mark Slevin

引用次数: 0

Natural Bletilla striata Polysaccharide-Based Hydrogels for Accelerating Hemostasis.

IF 5 3区化学

Gels Pub Date : 2025-01-08 DOI: 10.3390/gels11010048

Hui-Fang Lin, Yue-Yue Wang, Feng-Zhen Liu, Zi-Wei Yang, Hao Cui, Si-Yu Hu, Feng-He Li, Pei Pan

{"title":"Natural Bletilla striata Polysaccharide-Based Hydrogels for Accelerating Hemostasis.","authors":"Hui-Fang Lin, Yue-Yue Wang, Feng-Zhen Liu, Zi-Wei Yang, Hao Cui, Si-Yu Hu, Feng-He Li, Pei Pan","doi":"10.3390/gels11010048","DOIUrl":"10.3390/gels11010048","url":null,"abstract":"Most of the existing hydrogel dressings have inadequacies in mechanical performance, biological activities, compatibility, or versatility, which results in the development of rapid, green, and cost-effective approaches for hydrogels in biochemical and biomedical applications becoming a top-priority task. Herein, inspired by the inherent bioactivity, water retention properties, and biocompatibility of natural polysaccharide hydrogels, we have prepared self-healing gels. Using Bletilla striata polysaccharide (BSP), carboxymethyl chitosan (CMCS), and borax via borate ester linkages, we created hemostatic and self-healing Chinese herbal medicine hydrogels in varying concentrations (2.5%, 3.0%, and 4.0%). A rotational rheometer was used to describe the hydrogels' shape and rheological characteristics. At all concentrations, it was found that the hydrogels' elastic modulus (G') consistently and significantly outperformed their viscous modulus (G″), suggesting a robust internal structure. All of the hydrogels had cell viability levels as high as 100% and hemolysis rates below 1%, indicating the hydrogels' outstanding biocompatibility. Furthermore, the hydrogels demonstrated superior hemostatic qualities in an in vivo mouse tail amputation model, as well as in in vitro coagulation tests. The results show that the hydrogel possesses excellent self-healing properties, as well as a good biocompatibility and hemostatic performance, thus paving the way for the development of a potential hemostatic green hydrogel.","PeriodicalId":12506,"journal":{"name":"Gels","volume":"11 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Scalable Elastic Gelatin Hydrogel Films Crosslinked with Waterborne Polyurethane for Enhanced Mechanical Properties and Strain Recovery.

IF 5 3区化学

Gels Pub Date : 2025-01-08 DOI: 10.3390/gels11010049

Soon Mo Choi, Eun Joo Shin, Sun Mi Zo, Madhusudana Rao Kummara, Chul Min Kim, Anuj Kumar, Han Jo Bae, Ankur Sood, Sung Soo Han

{"title":"Development of Scalable Elastic Gelatin Hydrogel Films Crosslinked with Waterborne Polyurethane for Enhanced Mechanical Properties and Strain Recovery.","authors":"Soon Mo Choi, Eun Joo Shin, Sun Mi Zo, Madhusudana Rao Kummara, Chul Min Kim, Anuj Kumar, Han Jo Bae, Ankur Sood, Sung Soo Han","doi":"10.3390/gels11010049","DOIUrl":"10.3390/gels11010049","url":null,"abstract":"Exploiting novel crosslinking chemistry, this study pioneers the use of waterborne polyurethane (WPU) to chemically crosslink porcine-derived gelatin, producing enhanced gelatin hydrogel films through a solvent-casting method. Our innovative approach harnesses the reactive isocyanate groups of WPU, coupling them effectively with gelatin's hydroxyl and primary amino groups to form robust urea and urethane linkages within the hydrogel matrix. This method not only preserves the intrinsic elasticity of polyurethane but also significantly augments the films' tensile strength and strain. Comprehensive characterizations of these hydrogel films and pre-formed hydrogel reaction mixtures were conducted using viscosity measurements, Fourier Transform Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), and the universal testing machine (UTM) for tensile-recovery assessments, alongside evaluations of their biocompatibility. The results demonstrated a reduction in pore size with an increase in WPU concentration from 2 to 6% in the developed hydrogels with a decrease in the equilibrium swelling ratio from 15% to 9%, respectively. Further, hydrogels with 6% WPU exhibited the highest tensile stress in both a dry and wet state. The gelatin hydrogel formed with 6% WPU blend also demonstrated the growth and proliferation of CCD-986K (fibroblast) and CCD-1102 (keratinocyte) cells for up to 5 days of co-culturing. The results indicate a notable enhancement in the mechanical properties and biocompatibility of gelatin hydrogels upon the introduction of WPU, positioning these films as superior candidates for biomedical applications such as tissue engineering and wound dressing.","PeriodicalId":12506,"journal":{"name":"Gels","volume":"11 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Extrudable Hydrogels Based on Carboxymethyl Cellulose-Gelatin Complex Coacervates.

IF 5 3区化学

Gels Pub Date : 2025-01-08 DOI: 10.3390/gels11010051

Hamid Gharanjig, Hossein Najaf Zadeh, Campbell Stevens, Pram Abhayawardhana, Tim Huber, Ali Reza Nazmi

{"title":"Development of Extrudable Hydrogels Based on Carboxymethyl Cellulose-Gelatin Complex Coacervates.","authors":"Hamid Gharanjig, Hossein Najaf Zadeh, Campbell Stevens, Pram Abhayawardhana, Tim Huber, Ali Reza Nazmi","doi":"10.3390/gels11010051","DOIUrl":"10.3390/gels11010051","url":null,"abstract":"This study investigates the 3D extrusion printing of a carboxymethyl cellulose (CMC)-gelatin complex coacervate system. Various CMC-gelatin coacervate hydrogels were prepared and analyzed to achieve this goal. The impact of the CMC-gelatin ratio, pH, and total biopolymer concentration on coacervation formation and rheological properties was evaluated to characterize the printability of the samples. Turbidity results indicated that the molecular interactions between gelatin and CMC biopolymers are significantly pH-dependent, occurring within the range of pH 3.7 to pH 5.6 for the tested compositions. Confocal Laser Scanning Microscopy (CLSM) confirmed the presence of coacervates as spherical particles within the optimal coacervation range. Scanning electron microscopy micrographs supported the CLSM findings, revealing greater porosity within this optimal pH range. Rheological characterization demonstrated that all CMC-gelatin hydrogels exhibited pseudoplastic behavior, with an inverse correlation between increased coacervation and decreased shear viscosity. Additionally, the coacervates displayed lower tackiness compared to gelatin hydrogels, with the maximum tackiness normal force for various CMC-gelatin ratios ranging from 1 to 15 N, notably lower than the 29 N observed for gelatin hydrogels. Mixtures with CMC-gelatin ratios of 1:15 and 1:20 exhibited the best shear recovery behavior, maintaining higher strength after shear load. The maximum strength of the CMC-gelatin coacervate system was found at a biopolymer concentration of 6%. However, lower biopolymer content allowed for consistent extrusion. Importantly, all tested samples were successfully extruded at 22 ± 2 °C, with the 1:15 biopolymer ratio yielding the most consistent printed quality. Our research highlights the promise of the CMC-gelatin coacervate system for 3D printing applications, particularly in areas that demand precise material deposition and adjustable properties.","PeriodicalId":12506,"journal":{"name":"Gels","volume":"11 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Textural, Color, and Sensory Analysis of Cookies Prepared with Hemp Oil-Based Oleogels.

IF 5 3区化学

Gels Pub Date : 2025-01-07 DOI: 10.3390/gels11010046

Ana Leahu, Cristina Ghinea, Sorina Ropciuc, Cristina Damian

{"title":"Textural, Color, and Sensory Analysis of Cookies Prepared with Hemp Oil-Based Oleogels.","authors":"Ana Leahu, Cristina Ghinea, Sorina Ropciuc, Cristina Damian","doi":"10.3390/gels11010046","DOIUrl":"10.3390/gels11010046","url":null,"abstract":"The amount of saturated fat in cookies can be reduced by replacing margarine with oleogel, resulting in healthier products. In this study, the rheological and textural profile of cookies formulated with oleogel as the main margarine substitute was evaluated. Hemp seed vegetable oil was oleogelized with four types of waxes: beeswax (BW), carnauba wax (CW), candelilla wax (DW), rice bran wax (RW), and three oleogeling agents, sitosterol (S), pea protein (PP), and xanthan gum (XG), respectively. The textural and rheological properties of the oleogel dough samples were analyzed using the PertenTVT-6700 texturometer (Perten Instruments, Sweden) and the Haake rheometer. The results showed an increase in the hardness of cookie doughs with oleogels. The values of the elastic component (G') and the viscous component (G″) increased, which means that the oleogels used affected the rheological behavior at 25 °C, causing an increase in the dough consistency. Sensory attributes, texture, and color parameters of cookies with oleogels were determined. The cookies' hardness increased significantly from 4409.83 ± 0.13 g (control sample) to 7085.33 ± 0.15 g in the cookie sample prepared with hemp oil sitosterol oleogel, whereas the sample with candelilla wax had the lowest hardness value of 4048.09 ± 0.14 g. The color of the oleogel cookies was darker than that of the control cookies. The cookie sample with hemp oil and beeswax oleogel was the most appreciated by the evaluators among the oleogel cookie samples. The findings suggest that hemp seed oil oleogel is an effective fat substitute in cookies, promoting the application of this vegetable oil in food products.","PeriodicalId":12506,"journal":{"name":"Gels","volume":"11 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and Machine Learning-Driven Property Prediction of a Novel Hybrid Hydrogel Bioink Considering Extrusion-Based 3D Bioprinting.

IF 5 3区化学

Gels Pub Date : 2025-01-07 DOI: 10.3390/gels11010045

Rokeya Sarah, Kory Schimmelpfennig, Riley Rohauer, Christopher L Lewis, Shah M Limon, Ahasan Habib

{"title":"Characterization and Machine Learning-Driven Property Prediction of a Novel Hybrid Hydrogel Bioink Considering Extrusion-Based 3D Bioprinting.","authors":"Rokeya Sarah, Kory Schimmelpfennig, Riley Rohauer, Christopher L Lewis, Shah M Limon, Ahasan Habib","doi":"10.3390/gels11010045","DOIUrl":"10.3390/gels11010045","url":null,"abstract":"The field of tissue engineering has made significant advancements with extrusion-based bioprinting, which uses shear forces to create intricate tissue structures. However, the success of this method heavily relies on the rheological properties of bioinks. Most bioinks use shear-thinning. While a few component-based efforts have been reported to predict the viscosity of bioinks, the impact of shear rate has been vastly ignored. To address this gap, our research presents predictive models using machine learning (ML) algorithms, including polynomial fit (PF), decision tree (DT), and random forest (RF), to estimate bioink viscosity based on component weights and shear rate. We utilized novel bioinks composed of varying percentages of alginate (2-5.25%), gelatin (2-5.25%), and TEMPO-Nano fibrillated cellulose (0.5-1%) at shear rates from 0.1 to 100 s-1. Our study analyzed 169 rheological measurements using 80% training and 20% validation data. The results, based on the coefficient of determination (R2) and mean absolute error (MAE), showed that the RF algorithm-based model performed best: [(R2, MAE) RF = (0.99, 0.09), (R2, MAE) PF = (0.95, 0.28), (R2, MAE) DT = (0.98, 0.13)]. These predictive models serve as valuable tools for bioink formulation optimization, allowing researchers to determine effective viscosities without extensive experimental trials to accelerate tissue engineering.","PeriodicalId":12506,"journal":{"name":"Gels","volume":"11 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11765179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the Oxidative Stability of Beeswax-Canola Oleogels: Effects of Ascorbic Acid and Alpha-Tocopherol on Their Physical and Chemical Properties.

IF 5 3区化学

Gels Pub Date : 2025-01-07 DOI: 10.3390/gels11010043

Sonia Millao, Marcela Quilaqueo, Ingrid Contardo, Mónica Rubilar

{"title":"Enhancing the Oxidative Stability of Beeswax-Canola Oleogels: Effects of Ascorbic Acid and Alpha-Tocopherol on Their Physical and Chemical Properties.","authors":"Sonia Millao, Marcela Quilaqueo, Ingrid Contardo, Mónica Rubilar","doi":"10.3390/gels11010043","DOIUrl":"10.3390/gels11010043","url":null,"abstract":"The choice of antioxidant to be used in the formulation of an oleogel is crucial to determine its oxidative stability and functionality, as these factors can also affect the physical, chemical, and rheological properties of the oleogel. In this study, the effect of two antioxidants (ascorbic acid, AA, and alpha-tocopherol, AT) and their concentration (0.01, 0.02, and 0.03%) on the physical and chemical properties of beeswax and canola oil oleogels were evaluated. The results show that the type and concentration of antioxidants did not affect the thermal properties of the samples, and in FTIR analyses, no noticeable changes in spectra patterns are observed. Rheological analyses showed that the oleogels containing AA exhibited higher elasticity and resistance to deformation. Accelerated oxidative stability tests (storage at 50 °C and the Rancimat test) showed that AA effectively delayed oxidation. The induction time increased by 2.61-fold at higher concentrations, while AT did not significantly affect oxidation resistance. Overall, it was observed that AA improved oleogel firmness and OBC (up to 1.75-fold and 2.8%, respectively), whereas AT resulted in a softer and less stable gel structure. These results show the importance of antioxidant selection, indicating that hydrophilic antioxidants have promising applications in the formulation of beeswax oleogels.","PeriodicalId":12506,"journal":{"name":"Gels","volume":"11 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11765113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studying the Size-Dependence of Graphene Nanoplatelets (GNPs) in the Final Properties of Polyurethane Aerogels: Thermal Insulation and Mechanical Strength.

IF 5 3区化学

Gels Pub Date : 2025-01-07 DOI: 10.3390/gels11010044

Jaime Lledó, Judith Martín-de León, Tomás E Gómez Álvarez-Arenas, Miguel Ángel Rodríguez-Pérez, Beatriz Merillas

{"title":"Studying the Size-Dependence of Graphene Nanoplatelets (GNPs) in the Final Properties of Polyurethane Aerogels: Thermal Insulation and Mechanical Strength.","authors":"Jaime Lledó, Judith Martín-de León, Tomás E Gómez Álvarez-Arenas, Miguel Ángel Rodríguez-Pérez, Beatriz Merillas","doi":"10.3390/gels11010044","DOIUrl":"10.3390/gels11010044","url":null,"abstract":"In the present work, the influence of the addition of graphene nanoplatelets presenting different dimensions on polyurethane-polyisocyanurate aerogel structure and properties has been studied. The obtained aerogels synthesized through a sol-gel method have been fully characterized in terms of density, porosity, specific surface area, mechanical stiffness, thermal conductivity, and speed of sound. Opacified aerogels showing high porosity (>92%) and low densities (78-98 kg/m3) have been produced, and the effect of the size and content of graphene nanoplatelets has been studied. It has been observed that formulations with less than 5 wt.% of graphene nanoplatelets larger than 2 microns can effectively reduce the total thermal conductivity by absorption and scattering of the infrared radiation, reducing the heat transfer by this mechanism. The resulting opacified samples are highly insulating materials, with thermal conductivities less than 18 mW/m·K. Moreover, it has been observed that smaller particles with ca. 200 nm of average length can promote an increase in the elastic modulus, therefore obtaining stiffer aerogels, combined with thermal conductivities lower than 20 mW/m·K. Results have been studied in detail, providing a further understanding of the mechanisms for improving the final properties of these materials, making them more suitable for industrial applications.","PeriodicalId":12506,"journal":{"name":"Gels","volume":"11 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764653/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polydeoxynucleotide-Loaded Visible Light Photo-Crosslinked Gelatin Methacrylate Hydrogel: Approach to Accelerating Cartilage Regeneration.

IF 5 3区化学

Gels Pub Date : 2025-01-07 DOI: 10.3390/gels11010042

Sunjae Park, Youngjun Son, Jonggyu Park, Soyoon Lee, Na-Hyeon Kim, Se-Na Jang, Tae-Woong Kang, Jeong-Eun Song, Gilson Khang

{"title":"Polydeoxynucleotide-Loaded Visible Light Photo-Crosslinked Gelatin Methacrylate Hydrogel: Approach to Accelerating Cartilage Regeneration.","authors":"Sunjae Park, Youngjun Son, Jonggyu Park, Soyoon Lee, Na-Hyeon Kim, Se-Na Jang, Tae-Woong Kang, Jeong-Eun Song, Gilson Khang","doi":"10.3390/gels11010042","DOIUrl":"10.3390/gels11010042","url":null,"abstract":"Articular cartilage faces challenges in self-repair due to the lack of blood vessels and limited chondrocyte concentration. Polydeoxyribonucleotide (PDRN) shows promise for promoting chondrocyte growth and cartilage regeneration, but its delivery has been limited to injections. Continuous PDRN delivery is crucial for effective cartilage regeneration. This study explores using gelatin methacrylate (gelMA) hydrogel, crosslinked with visible light and riboflavin 5'-phosphate sodium (RF) as a photoinitiator, for sustained PDRN release. GelMA hydrogel's synthesis was confirmed through spectrophotometric techniques, demonstrating successful methacrylate group incorporation. PDRN-loaded gelMA hydrogels displayed varying pore sizes, swelling ratios, degradation rates, and mechanical properties based on gelMA concentration. They showed sustained PDRN release and biocompatibility, with the 14% gelMA-PDRN composition performing best. Glycosaminoglycan (GAG) activity was higher in PDRN-loaded hydrogels, indicating a positive effect on cartilage formation. RT-PCR analysis revealed increased expression of cartilage-specific genes (COL2, SOX9, AGG) in gelMA-PDRN. Histological assessments in a rabbit cartilage defect model demonstrated superior regenerative effects of gelMA-PDRN hydrogels. This study highlights the potential of gelMA-PDRN hydrogels in cartilage tissue engineering, providing a promising approach for effective cartilage regeneration.","PeriodicalId":12506,"journal":{"name":"Gels","volume":"11 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11765300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Methacrylated Gellan Gum 3D Bioprinted Patches Loaded with Tannic Acid or L-Ascorbic Acid as Potential Platform for Wound Dressing Application.

IF 5 3区化学

Gels Pub Date : 2025-01-05 DOI: 10.3390/gels11010040

Federica Scalia, Alessandra Maria Vitale, Domiziana Picone, Noemi De Cesare, Maria Swiontek Brzezinska, Beata Kaczmarek-Szczepanska, Alfredo Ronca, Barbara Zavan, Fabio Bucchieri, Marta Anna Szychlinska, Ugo D'Amora

{"title":"Exploring Methacrylated Gellan Gum 3D Bioprinted Patches Loaded with Tannic Acid or L-Ascorbic Acid as Potential Platform for Wound Dressing Application.","authors":"Federica Scalia, Alessandra Maria Vitale, Domiziana Picone, Noemi De Cesare, Maria Swiontek Brzezinska, Beata Kaczmarek-Szczepanska, Alfredo Ronca, Barbara Zavan, Fabio Bucchieri, Marta Anna Szychlinska, Ugo D'Amora","doi":"10.3390/gels11010040","DOIUrl":"10.3390/gels11010040","url":null,"abstract":"To improve wound healing, advanced biofabrication techniques are proposed here to develop customized wound patches to release bioactive agents targeting cell function in a controlled manner. Three-dimensional (3D) bioprinted \"smart\" patches, based on methacrylated gellan gum (GGMA), loaded with tannic acid (TA) or L-ascorbic acid (AA) have been manufactured. To improve stability and degradation time, gellan gum (GG) was chemically modified by grafting methacrylic moieties on the polysaccharide backbone. GGMA patches were characterized through physicochemical, morphological and mechanical evaluation. Kinetics release and antioxidant potential of TA and AA as well as antimicrobial activity against common pathogens Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli in accordance with ISO 22196:2011 are reported. The cytocompatibility of the patches was demonstrated by direct and indirect tests on human dermal fibroblasts (HDF) as per ISO 10993. The positive effect of GGMA patches on cell migration was assessed through a wound healing assay. The results highlighted that the patches are cytocompatible, speed up wound healing and can swell upon contact with the hydration medium and release TA and AA in a controlled way. Overall, the TA- and AA-loaded GGMA patches demonstrated suitable mechanical features; no cytotoxicity; and antioxidant, antimicrobial and wound healing properties, showing satisfactory potential for wound dressing applications.","PeriodicalId":12506,"journal":{"name":"Gels","volume":"11 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11765158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0