Frontiers in Neural Circuits最新文献_第9页

Frontiers | A brief history of somatostatin interneuron taxonomy or: how many somatostatin subtypes are there, really? 前沿 | 体生长抑素中间神经元分类简史或：到底有多少种体生长抑素亚型？

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-06-28 DOI: 10.3389/fncir.2024.1436915

Ariel Agmon, Alison L. Barth

{"title":"Frontiers | A brief history of somatostatin interneuron taxonomy or: how many somatostatin subtypes are there, really?","authors":"Ariel Agmon, Alison L. Barth","doi":"10.3389/fncir.2024.1436915","DOIUrl":"https://doi.org/10.3389/fncir.2024.1436915","url":null,"abstract":"We provide a brief (and unabashedly biased) overview of the pre-transcriptomic history of somatostatin interneuron taxonomy, followed by a chronological summary of the large-scale, NIH-supported effort over the last ten years to generate a comprehensive, single-cell RNA-seq-based taxonomy of cortical neurons. Focusing on somatostatin interneurons, we present the perspective of experimental neuroscientists trying to incorporate the new classification schemes into their own research while struggling to keep up with the ever-increasing number of proposed cell types, which seems to double every two years. We suggest that for experimental analysis, the most useful taxonomic level is the subdivision of somatostatin interneurons into ten or so “supertypes,” which closely agrees with their more traditional classification by morphological, electrophysiological and neurochemical features. We argue that finer subdivisions (“t-types” or “clusters”), based on slight variations in gene expression profiles but lacking clear phenotypic differences, are less useful to researchers and may actually defeat the purpose of classifying neurons to begin with. We end by stressing the need for generating novel tools (mouse lines, viral vectors) for genetically targeting distinct supertypes for expression of fluorescent reporters, calcium sensors and excitatory or inhibitory opsins, allowing neuroscientists to chart the input and output synaptic connections of each proposed subtype, reveal the position they occupy in the cortical network and examine experimentally their roles in sensorimotor behaviors and cognitive brain functions.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":"10 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141722041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Odors in space 空间异味

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-06-24 DOI: 10.3389/fncir.2024.1414452

Olivia McKissick, Nell Klimpert, Jason T. Ritt, Alexander Fleischmann

引用次数: 0

Frontiers | Brain image data processing using collaborative data workflows on Texera 前沿｜利用 Texera 上的协作数据工作流处理脑图像数据

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-06-20 DOI: 10.3389/fncir.2024.1398884

Yunyan Ding, Yicong Huang, Pan Gao, Andy Thai, Atchuth Naveen Chilaparasetti, M. Gopi, Xiangmin Xu, Chen Li

{"title":"Frontiers | Brain image data processing using collaborative data workflows on Texera","authors":"Yunyan Ding, Yicong Huang, Pan Gao, Andy Thai, Atchuth Naveen Chilaparasetti, M. Gopi, Xiangmin Xu, Chen Li","doi":"10.3389/fncir.2024.1398884","DOIUrl":"https://doi.org/10.3389/fncir.2024.1398884","url":null,"abstract":"In the realm of neuroscience, mapping the three-dimensional (3D) neural circuitry and architecture of the brain is important for advancing our understanding of neural circuit organization and function. This study presents a novel pipeline that transforms mouse brain samples into detailed 3D brain models using a collaborative data analytics platform called “Texera.” The user-friendly Texera platform allows for effective interdisciplinary collaboration between team members in neuroscience, computer vision, and data processing. Our pipeline utilizes the tile images from a serial two-photon tomography/TissueCyte system, then stitches tile images into brain section images, and constructs 3D whole-brain image datasets. The resulting 3D data supports downstream analyses, including 3D whole-brain registration, atlas-based segmentation, cell counting, and high-resolution volumetric visualization. Using this platform, we implemented specialized optimization methods and obtained significant performance enhancement in workflow operations. We expect the neuroscience community can adopt our approach for large-scale image-based data processing and analysis.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":"31 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141577863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Anesthetized animal experiments for neuroscience research 用于神经科学研究的麻醉动物实验

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-05-31 DOI: 10.3389/fncir.2024.1426689

Shin Nagayama, Sanae Hasegawa-Ishii, Shu Kikuta

{"title":"Anesthetized animal experiments for neuroscience research","authors":"Shin Nagayama, Sanae Hasegawa-Ishii, Shu Kikuta","doi":"10.3389/fncir.2024.1426689","DOIUrl":"https://doi.org/10.3389/fncir.2024.1426689","url":null,"abstract":"Brain research has progressed with anesthetized animal experiments for a long time. Recent progress in research techniques allows us to measure neuronal activity in awake animals combined with behavioral tasks. The trends became more prominent in the last decade. This new research style triggers the paradigm shift in the research of brain science, and new insights into brain function have been revealed. It is reasonable to consider that awake animal experiments are more ideal for understanding naturalistic brain function than anesthetized ones. However, the anesthetized animal experiment still has advantages in some experiments. To take advantage of the anesthetized animal experiments, it is important to understand the mechanism of anesthesia and carefully handle the obtained data. In this minireview, we will shortly summarize the molecular mechanism of anesthesia in animal experiments, a recent understanding of the neuronal activities in a sensory system in the anesthetized animal brain, and consider the advantages and disadvantages of the anesthetized and awake animal experiments. This discussion will help us to use both research conditions in the proper manner.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":"37 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endogenous opioids in the olfactory tubercle and their roles in olfaction and quality of life 嗅结节中的内源性阿片类物质及其在嗅觉和生活质量中的作用

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-05-30 DOI: 10.3389/fncir.2024.1408189

Koshi Murata, Ayako Maegawa, Yoshimasa Imoto, Shigeharu Fujieda, Yugo Fukazawa

{"title":"Endogenous opioids in the olfactory tubercle and their roles in olfaction and quality of life","authors":"Koshi Murata, Ayako Maegawa, Yoshimasa Imoto, Shigeharu Fujieda, Yugo Fukazawa","doi":"10.3389/fncir.2024.1408189","DOIUrl":"https://doi.org/10.3389/fncir.2024.1408189","url":null,"abstract":"Olfactory dysfunctions decrease daily quality of life (QOL) in part by reducing the pleasure of eating. Olfaction plays an essential role in flavor sensation and palatability. The decreased QOL due to olfactory dysfunction is speculated to result from abnormal neural activities in the olfactory and limbic areas of the brain, as well as peripheral odorant receptor dysfunctions. However, the specific underlying neurobiological mechanisms remain unclear. As the olfactory tubercle (OT) is one of the brain’s regions with high expression of endogenous opioids, we hypothesize that the mechanism underlying the decrease in QOL due to olfactory dysfunction involves the reduction of neural activity in the OT and subsequent endogenous opioid release in specialized subregions. In this review, we provide an overview and recent updates on the OT, the endogenous opioid system, and the pleasure systems in the brain and then discuss our hypothesis. To facilitate the effective treatment of olfactory dysfunctions and decreased QOL, elucidation of the neurobiological mechanisms underlying the pleasure of eating through flavor sensation is crucial.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":"90 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shaping the olfactory map: cell type-specific activity patterns guide circuit formation 塑造嗅觉图谱：细胞类型特异性活动模式引导电路形成

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-05-27 DOI: 10.3389/fncir.2024.1409680

Ai Nakashima, Haruki Takeuchi

引用次数: 0

Hormonal and circuit mechanisms controlling female sexual behavior 控制女性性行为的荷尔蒙和回路机制

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-05-01 DOI: 10.3389/fncir.2024.1409349

Sayaka Inoue

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Comparison of the connectivity of the posterior intralaminar thalamic nucleus and peripeduncular nucleus in rats and mice 比较大鼠和小鼠丘脑后束内核和周核的连接性

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-04-26 DOI: 10.3389/fncir.2024.1384621

Hui-Ru Cai, Sheng-Qiang Chen, Xiao-Jun Xiang, Xue-Qin Zhang, Run-Zhe Ma, Ge Zhu, Song-Lin Ding

{"title":"Comparison of the connectivity of the posterior intralaminar thalamic nucleus and peripeduncular nucleus in rats and mice","authors":"Hui-Ru Cai, Sheng-Qiang Chen, Xiao-Jun Xiang, Xue-Qin Zhang, Run-Zhe Ma, Ge Zhu, Song-Lin Ding","doi":"10.3389/fncir.2024.1384621","DOIUrl":"https://doi.org/10.3389/fncir.2024.1384621","url":null,"abstract":"The posterior intralaminar thalamic nucleus (PIL) and peripeduncular nucleus (PP) are two adjoining structures located medioventral to the medial geniculate nucleus. The PIL-PP region plays important roles in auditory fear conditioning and in social, maternal and sexual behaviors. Previous studies often lumped the PIL and PP into single entity, and therefore it is not known if they have common and/or different brain-wide connections. In this study, we investigate brain-wide efferent and afferent projections of the PIL and PP using reliable anterograde and retrograde tracing methods. Both PIL and PP project strongly to lateral, medial and anterior basomedial amygdaloid nuclei, posteroventral striatum (putamen and external globus pallidus), amygdalostriatal transition area, zona incerta, superior and inferior colliculi, and the ectorhinal cortex. However, the PP rather than the PIL send stronger projections to the hypothalamic regions such as preoptic area/nucleus, anterior hypothalamic nucleus, and ventromedial nucleus of hypothalamus. As for the afferent projections, both PIL and PP receive multimodal information from auditory (inferior colliculus, superior olivary nucleus, nucleus of lateral lemniscus, and association auditory cortex), visual (superior colliculus and ectorhinal cortex), somatosensory (gracile and cuneate nuclei), motor (external globus pallidus), and limbic (central amygdaloid nucleus, hypothalamus, and insular cortex) structures. However, the PP rather than PIL receives strong projections from the visual related structures parabigeminal nucleus and ventral lateral geniculate nucleus. Additional results from Cre-dependent viral tracing in mice have also confirmed the main results in rats. Together, the findings in this study would provide new insights into the neural circuits and functional correlation of the PIL and PP.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":"312 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140805823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunolabeling-compatible PEGASOS tissue clearing for high-resolution whole mouse brain imaging 用于高分辨率小鼠全脑成像的免疫标记兼容 PEGASOS 组织清除技术

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-04-17 DOI: 10.3389/fncir.2024.1345692

Pan Gao, Matthew Rivera, Xiaoxiao Lin, Todd C. Holmes, Hu Zhao, Xiangmin Xu

{"title":"Immunolabeling-compatible PEGASOS tissue clearing for high-resolution whole mouse brain imaging","authors":"Pan Gao, Matthew Rivera, Xiaoxiao Lin, Todd C. Holmes, Hu Zhao, Xiangmin Xu","doi":"10.3389/fncir.2024.1345692","DOIUrl":"https://doi.org/10.3389/fncir.2024.1345692","url":null,"abstract":"Novel brain clearing methods revolutionize imaging by increasing visualization throughout the brain at high resolution. However, combining the standard tool of immunostaining targets of interest with clearing methods has lagged behind. We integrate whole-mount immunostaining with PEGASOS tissue clearing, referred to as iPEGASOS (immunostaining-compatible PEGASOS), to address the challenge of signal quenching during clearing processes. iPEGASOS effectively enhances molecular-genetically targeted fluorescent signals that are otherwise compromised during conventional clearing procedures. Additionally, we demonstrate the utility of iPEGASOS for visualizing neurochemical markers or viral labels to augment visualization that transgenic mouse lines cannot provide. Our study encompasses three distinct applications, each showcasing the versatility and efficacy of this approach. We employ whole-mount immunostaining to enhance molecular signals in transgenic reporter mouse lines to visualize the whole-brain spatial distribution of specific cellular populations. We also significantly improve the visualization of neural circuit connections by enhancing signals from viral tracers injected into the brain. Last, we show immunostaining without genetic markers to selectively label beta-amyloid deposits in a mouse model of Alzheimer’s disease, facilitating the comprehensive whole-brain study of pathological features.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":"47 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140612867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neural basis for pheromone signal transduction in mice 小鼠信息素信号转导的神经基础

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-04-15 DOI: 10.3389/fncir.2024.1409994

Ken Murata, Takumi Itakura, Kazushige Touhara

{"title":"Neural basis for pheromone signal transduction in mice","authors":"Ken Murata, Takumi Itakura, Kazushige Touhara","doi":"10.3389/fncir.2024.1409994","DOIUrl":"https://doi.org/10.3389/fncir.2024.1409994","url":null,"abstract":"Pheromones are specialized chemical messengers used for inter-individual communication within the same species, playing crucial roles in modulating behaviors and physiological states. The detection mechanisms of these signals at the peripheral organ and their transduction to the brain have been unclear. However, recent identification of pheromone molecules, their corresponding receptors, and advancements in neuroscientific technology have started to elucidate these processes. In mammals, the detection and interpretation of pheromone signals are primarily attributed to the vomeronasal system, which is a specialized olfactory apparatus predominantly dedicated to decoding socio-chemical cues. In this mini-review, we aim to delineate the vomeronasal signal transduction pathway initiated by specific vomeronasal receptor-ligand interactions in mice. First, we catalog the previously identified pheromone ligands and their corresponding receptor pairs, providing a foundational understanding of the specificity inherent in pheromonal communication. Subsequently, we examine the neural circuits involved in processing each pheromone signal. We focus on the anatomical pathways, the sexually dimorphic and physiological state-dependent aspects of signal transduction, and the neural coding strategies underlying behavioral responses to pheromonal cues. These insights provide further critical questions regarding the development of innate circuit formation and plasticity within these circuits.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":"16 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140811547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0