Fungal Genetics Reports最新文献

{"title":"An effect of homology length on gene disruption in Neurospora crassa","authors":"Y. Nakanishi, C. Ishii, H. Inoue","doi":"10.4148/1941-4765.1120","DOIUrl":"https://doi.org/10.4148/1941-4765.1120","url":null,"abstract":"For targeted gene disruption in wild-type Neurospora crassa, 1000-bp of homologous sequences on either side of the cassette used for disruption is sufficient to give more than 10 % homologous recombination. We report here that varying the length of homology on each side seems to have different effects on the homologous recombination frequency. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol52/iss1/1","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"132 1","pages":"5-6"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75862516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Ligation-PCR Approach for Generating Gene Replacement Constructs in Magnaporthe grisea","authors":"Xinhua Zhao, C. Xue, Yangseon Kim, Jin-Rong Xu","doi":"10.4148/1941-4765.1137","DOIUrl":"https://doi.org/10.4148/1941-4765.1137","url":null,"abstract":"The conventional approach for generating gene replacement constructs involves several sequence-specific cloning steps and is time-consuming. A ligation-PCR approach was developed to efficiently generate gene replacement constructs. Two vectors useful for this ligation-PCR approach and another vector suitable for improving the efficiency of knockout mutant screens were constructed. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/7","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"2016 1","pages":"17-18"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86552946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive Isolation: Evidence that Ascobolus stercorarius and Ascobolus furfuraceus are two species, not one","authors":"G. Bistis","doi":"10.4148/1941-4765.1140","DOIUrl":"https://doi.org/10.4148/1941-4765.1140","url":null,"abstract":"Strains obtained from the germination of 30-40 year old ascospores from seven stocks of the heterothallicAscobolus stercorarius furfuraceous complex were mated in all combinations. All st X st and fu X fu pairings were fertile (ascospores) whereas all st X fu pairings were sterile (no ascospores). Based on this reproductive isolation between st and fu strains I conclude that the complex consists of two species. The block that segregated the two species is probably some stage in the establishment of the dikaryotic phase in ascogenous hyphae. One hypothesis is that this block is at the stage of nuclear recognition. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/10","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"25 1","pages":"23-25"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88779141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A precise size-estimate for the small RNA products arising from Neurospora crassa Dicer activity","authors":"P. Refalo, M. Sachs","doi":"10.4148/1941-4765.1139","DOIUrl":"https://doi.org/10.4148/1941-4765.1139","url":null,"abstract":"Neurospora crassa cell-free extracts prepared from strains containing one or both functional Dicer genes, but not from a strain lacking functional Dicer genes, converts radiolabeled double-strand RNA (dsRNA) in an energy-dependent manner into short RNAs with an estimated size of ~25-nt (Catalanotto et al. 2004). A smaller nucleolytic digestion product was also produced in an energy-dependent manner from either dsRNA or single-stranded RNA. Here we obtained more precise sizes for these products by electrophoresis of samples on a long (40-cm) denaturing DNA sequencing gel (20% polyacrylamide/7M urea). Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/9","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"10 1","pages":"21-22"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88514183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inverted Race Tube Assay for Circadian Clock Studies of the Neurospora Accessions","authors":"Sohyun Park, Kwangwon Lee","doi":"10.4148/1941-4765.1135","DOIUrl":"https://doi.org/10.4148/1941-4765.1135","url":null,"abstract":"Although the Neurospora crassa circadian clock has been studied for forty years, population studies of natural accessions have been limited by technical difficulties associated with the conventional race tube assay (CRTA) that is used to measure asexual development (conidiation). Due to the buildup of CO2 in the CRTA that represses banding, a mutant strain band (bd) has been utilized for increased visualization of the banding phenotype. In order to study the circadian clock in natural accessions of Neurospora multiple techniques have been explored. One such technique, the rubidium chloride-supplemented race tube assay (RRTA) has been used successfully. Here we present a new technique, the Inverted Race Tube Assay (IRTA) that is a simple modification of the CRTA. We analyzed 5 natural accessions of Neurospora using CRTA, IRTA and RRTA and discuss the advantages of the IRTA in natural variation studies in Neurospora. This regular paper is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol51/iss1/5 12 Fungal Genetics Newsletter Inverted Race Tube Assay for Circadian Clock Studies of the Neurospora Accessions. Sohyun Park, Kwangwon Lee. Department of Plant Pathology, Cornell University, Ithaca NY 14850 Fungal Genet. Newsl. 51:12-14 Although the Neurospora crassa circadian clock has been studied for forty years, population studies of natural accessions have been limited by technical difficulties associated with the conventional race tube assay (CRTA) that is used to measure asexual development (conidiation). Due to the buildup of CO2 in the CRTA that represses banding, a mutant strain band (bd) has been utilized for increased visualization of the banding phenotype. In order to study the circadian clock in natural accessions of Neurospora multiple techniques have been explored. One such technique, the rubidium chloride-supplemented race tube assay (RRTA) has been used successfully. Here we present a new technique, the Inverted Race Tube Assay (IRTA) that is a simple modification of the CRTA. We analyzed 5 natural accessions of Neurospora using CRTA, IRTA and RRTA and discuss the advantages of the IRTA in natural variation studies in Neurospora. N. crassa has proved to be a successful model for studying the molecular bases of circadian clocks. Significant insights into the mechanism of the clock have been obtained in N. crassa including a) transcriptional/translational feedback loops in the clock that opened up the conceptual foundation for our understanding of the biological clock (Aronson et al.1994); b) the resetting mechanisms by light and temperature (Liu et al. 1998; Crosthwaite et al. 1995); c) circadian gating of cellular activities (Heintzen et al. 2001); and d) the involvement of anti-sense RNA in the circadian regulatory circuit (Kramer et al. 2003). A critical step in studying the circadian clock is the ability to easily observe and measure circadian regulated behavior. The rhythmic behavior that has been most extensiv","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"12 1","pages":"12-14"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85666415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bird Medium: an alternative to Vogel Medium","authors":"R. L. Metzenberg","doi":"10.4148/1941-4765.1138","DOIUrl":"https://doi.org/10.4148/1941-4765.1138","url":null,"abstract":"This medium was designed to circumvent some problems that arise in the use of Medium N (Vogel 1964 Am. Naturalist 98:435-446). These are, among others, the presence of high levels of citrate, a chelator which leaves the concentration of calcium and trace elements uncertain; the use of ammonium nitrate, which leaves the actual source of nitrogen ambiguous; the use of MgSO4, which does not allow the experimenter to vary the concentration of magnesium and sulfur independently; the high activity coefficient for the pKa values of citrate, which makes the pH unnecessarily sensitive to ionic strength; the use of sucrose, which leaves uncertain the nature and relative amounts of the hexose(s) being used at any particular moment; the need to use chloroform as a preservative, which results in the gradual depletion of the aqueous phase of complexes of trace elements. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/8","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"4 1","pages":"19-20"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75424604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why Study Schizophyllum","authors":"C. Raper, T. Fowler","doi":"10.4148/1941-4765.1142","DOIUrl":"https://doi.org/10.4148/1941-4765.1142","url":null,"abstract":"The process of mating, fertilization, fruiting, meiosis and spore formation is regulated by two kinds of genetic factors residing at the A and B mating-type loci, earlier called incompatibility factors A and B. Over the eight decades since Kniep's discovery, revelations about the genetic, biochemical and molecular underpinnings of this bizarre system have made an exciting story (see list of selected references, below). While other interesting aspects of Schizophyllum have been explored, notably the hydrophobins of Wessels and associates (reviewed in W essels, 2000), a principal focus over the years has been on mating compatibility and sexual development. Although Schizophyllum commune 's main role in nature is to recycle carbon by breaking down celluose and xylans in fallen wood (Clarke and Yaguchi, 1986; Bray and Clarke, 1995), it has been documented occasionally as a pathogen in fruit orchards (Latham, 1970; Oprea, et al, 1995) and also in immunologically compromised humans (Buzina et al, 2001).","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"63 1.2 1","pages":"30-36"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91165168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Another inconsistency in the pedigree of the Oak Ridge wild types of Neurospora crassa","authors":"O. Gavric, A. Griffiths","doi":"10.4148/1941-4765.1134","DOIUrl":"https://doi.org/10.4148/1941-4765.1134","url":null,"abstract":"A multiple-site molecular dimorphism for the gene for phospholipase C has no known evolutionary basis, yet reveals that Oak Ridge wild types cannot have originated as described in the current best pedigree. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/4","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"62 1","pages":"9-11"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83693587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wild type Neurospora crassa strains preferred for use as standards","authors":"D. D. Perkins","doi":"10.4148/1941-4765.1133","DOIUrl":"https://doi.org/10.4148/1941-4765.1133","url":null,"abstract":"The highly inbred Neurospora crassa strains 74-OR23-1VA (FGSC 2489) and 74-ORS-6a (FGSC 4200) are recommended for use as standard wild types. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/3","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"25 1","pages":"7-8"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78444090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PCR-based markers for genetic mapping in Neurospora crassa.","authors":"Moshi Kotierk, Myron L Smith","doi":"10.4148/1941-4765.1141","DOIUrl":"https://doi.org/10.4148/1941-4765.1141","url":null,"abstract":"Eighteen PCR-based markers are described for use in mapping mutations in Oak Ridge background strains of Neurospora crassa. These markers are located on each of the seven linkage groups and in the mitochondrial genome to enable course-scale linkage mapping. Following mapping to a linkage group, additional markers can be developed in the co-segregating region for fine-scale mapping of mutations. As with the N. crassa RFLP map (Metzenberg and Grotelueschen, 1993; Nelson and Perkins, 2000), the addition of PCR-based markers by members of the Neurospora community will enhance this marker set for mapping purposes. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/11 26 Fungal Genetics Newsletter PCR-based markers for genetic mapping in Neurospora crassa . Moshi Kotierk and Myron L. Smith, Carleton University, Ottawa-Carleton Institute of Biology, 1125 Colonel By Drive, Ottawa, Ontario, K1S 5B6, Canada Fungal Genetics Newsletter 51:26-26 Eighteen PCR-based markers are described for use in mapping mutations in Oak Ridge background strains of Neurospora crassa . These markers are located on each of the seven linkage groups and in the mitochondrial genome to enable course-scale linkage mapping. Following mapping to a linkage group, additional markers can be developed in the co-segregating region for fine-scale mapping of mutations. As with the N. crassa RFLP map (Metzenberg and Grotelueschen, 1993; Nelson and Perkins, 2000), the addition of PCR-based markers by members of the Neurospora community will enhance this marker set for mapping purposes. Map-based or positional cloning is necessary to locate, identify and characterize genes that are associated with spontaneous or induced mutations. Methods available for locating mutations in Neurospora crassa involve co-segregation analysis using phenotypic (e.g. auxotrophic) markers. For example, multiply marked centromere tester strains of N. crassa are available for this purpose through the Fungal Genetics Stock Center (e.g. Perkins, 1972; Metzenberg et al., 1984). Often, however, co-segregation analysis with a set of phenotypic markers will not provide adequate resolution for the fine-scale linkage mapping necessary to clone a trait of interest and may require subsequent crosses to achieve further resolution. In this note, PCR-based markers are described for locating mutations generated in Oak Ridge background strains. The method takes advantage of abundant sequence differences between Oak Ridge and M auriceville genetic backgrounds, and the recently completed N. crassa genome sequence of the Oak Ridge standard strain 74-OR23-1VA (Galagan et al., 2003). The PCR-based markers are distributed throughout the seven N. crassa linkage groups and the mitochondrial DNA (Figure 1). Mapping of a mutation involves four steps. 1) Crossing an Oak Ridge-background ","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"386 1","pages":"26-29"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77123904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}