{"title":"粗神经孢子虫活性产生的小RNA产物的精确大小估计","authors":"P. Refalo, M. Sachs","doi":"10.4148/1941-4765.1139","DOIUrl":null,"url":null,"abstract":"Neurospora crassa cell-free extracts prepared from strains containing one or both functional Dicer genes, but not from a strain lacking functional Dicer genes, converts radiolabeled double-strand RNA (dsRNA) in an energy-dependent manner into short RNAs with an estimated size of ~25-nt (Catalanotto et al. 2004). A smaller nucleolytic digestion product was also produced in an energy-dependent manner from either dsRNA or single-stranded RNA. Here we obtained more precise sizes for these products by electrophoresis of samples on a long (40-cm) denaturing DNA sequencing gel (20% polyacrylamide/7M urea). Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/9","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"10 1","pages":"21-22"},"PeriodicalIF":0.0000,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"A precise size-estimate for the small RNA products arising from Neurospora crassa Dicer activity\",\"authors\":\"P. Refalo, M. Sachs\",\"doi\":\"10.4148/1941-4765.1139\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Neurospora crassa cell-free extracts prepared from strains containing one or both functional Dicer genes, but not from a strain lacking functional Dicer genes, converts radiolabeled double-strand RNA (dsRNA) in an energy-dependent manner into short RNAs with an estimated size of ~25-nt (Catalanotto et al. 2004). A smaller nucleolytic digestion product was also produced in an energy-dependent manner from either dsRNA or single-stranded RNA. Here we obtained more precise sizes for these products by electrophoresis of samples on a long (40-cm) denaturing DNA sequencing gel (20% polyacrylamide/7M urea). Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/9\",\"PeriodicalId\":12490,\"journal\":{\"name\":\"Fungal Genetics Reports\",\"volume\":\"10 1\",\"pages\":\"21-22\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2004-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fungal Genetics Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4148/1941-4765.1139\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fungal Genetics Reports","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4148/1941-4765.1139","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
摘要
从含有一个或两个功能Dicer基因的菌株制备的粗神经孢子虫无细胞提取物，而不是从缺乏功能Dicer基因的菌株制备的提取物，以能量依赖的方式将放射性标记的双链RNA (dsRNA)转化为估计大小为~25 nt的短RNA (Catalanotto et al. 2004)。从dsRNA或单链RNA中也以能量依赖的方式产生较小的溶核消化产物。在这里，我们通过在长(40厘米)变性DNA测序凝胶(20%聚丙烯酰胺/7M尿素)上对样品进行电泳，获得了这些产品更精确的尺寸。本作品采用知识共享署名-相同方式共享4.0许可协议。这篇常规论文可在真菌遗传学报告:http://newprairiepress.org/fgr/vol51/iss1/9
A precise size-estimate for the small RNA products arising from Neurospora crassa Dicer activity
Neurospora crassa cell-free extracts prepared from strains containing one or both functional Dicer genes, but not from a strain lacking functional Dicer genes, converts radiolabeled double-strand RNA (dsRNA) in an energy-dependent manner into short RNAs with an estimated size of ~25-nt (Catalanotto et al. 2004). A smaller nucleolytic digestion product was also produced in an energy-dependent manner from either dsRNA or single-stranded RNA. Here we obtained more precise sizes for these products by electrophoresis of samples on a long (40-cm) denaturing DNA sequencing gel (20% polyacrylamide/7M urea). Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/9
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