{"title":"Neurospora msh4 ortholog confirmed by split-marker deletion","authors":"Sue Conway, F. Bowring, J. Yeadon, D. Catcheside","doi":"10.4148/1941-4765.1105","DOIUrl":"https://doi.org/10.4148/1941-4765.1105","url":null,"abstract":"Although most eukaryotes have both MSH4 and MSH5 orthologs, Neurospora was initially thought to lack msh-4. We have deleted the most likely msh-4 candidate and observed a delay in the sexual cycle, disruption to meiosis and a reduction in fertility. Deletion is dominant, showing msh-4 is subject to MSUD. We conclude that Neurospora has a MSH4 ortholog and that it may have remained undetected because of an unusually high number of introns. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol53/iss1/2","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"29 1","pages":"5-8"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73708938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Aspergillus Bibliography 2006","authors":"J. Clutterbuck","doi":"10.4148/1941-4765.1116","DOIUrl":"https://doi.org/10.4148/1941-4765.1116","url":null,"abstract":"This bibliography attempts to cover genetical and biochemical publications on Aspergillus nidulans and also includes selected references to related species and topics. Entries have been checked as far as possible, but please tell me of any errors and omissions. Authors are kindly requested to send a copy of each article to the FGSC for its reprint collection. This bibliography is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol53/iss1/13 ASPERGILLUS BIBLIOGRAPHY 2006 This bibliography attempts to cover genetical and biochemical publications on Aspergillus nidulans and also includes selected references to related species and topics. Entries have been checked as far as possible, but please tell me of any errors and omissions. Authors are kindly requested to send a copy of each article to the FGSC for its reprint collection. John Clutterbuck. Institute of Biomedical and Life Sciences, Anderson College, University of Glasgow, Glasgow G11 6NU, Scotland, UK. Email: j.clutterbuck@bio.gla.ac.uk","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"78 1","pages":"13"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88571992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Supplement to Neurospora bibliographies: Chrysonilia citations","authors":"Craig Wilson","doi":"10.4148/1941-4765.1129","DOIUrl":"https://doi.org/10.4148/1941-4765.1129","url":null,"abstract":"David Perkins pointed out to me that research articles dealing with Neurospora do not always include the word Neurospora. There are instances where the fungus is named Chrysonilia sp. (usually C. sitophila), referring to the imperfect stage. Here follows a list of such citations, with a few caveats. In many cases, the journals in which these articles appeared are not available to me, so I generally have not confirmed these citations. Some may be only single-page abstracts. I have not attempted to find all citations using Monilia sitophila, a name in use before Chrysonilia. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This bibliography is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol52/iss1/10 Supplement to Neurospora bibliographies: Chrysonilia citations Craig Wilson – Northwest Fisheries Science Center, Seattle WA 98112 David Perkins pointed out to me that research articles dealing with Neurospora do not always include the word Neurospora. There are instances where the fungus is named Chrysonilia sp. (usually C. sitophila), referring to the imperfect stage. Here follows a list of such citations, with a few caveats. In many cases, the journals in which these articles appeared are not available to me, so I generally have not confirmed these citations. Some may be only single-page abstracts. I have not attempted to find all citations using Monilia sitophila, a name in use before Chrysonilia. 1. Abdel-Rahman, T. M. A., M. Salama A-A, M. I. A. Ali, and N. A. H. Tharwat. 1990. Fibrinolytic activity of some fungi isolated from self-heated composted fertilizer. Botanical Magazine Tokyo 103:313-324. 2. Ahmad, M. S., and M. A. Malik. 1997. Formulation of a synthetic medium for the production of antifungal antibiotic from Bacillus subtilis AECL 69. Pakistan Journal of Zoology 29:15-21. 3. Aidoo, K. E., A. Anderton, and K. A. Milligan. 1995. A 2-year survey of the airborne mycoflora in a hospital environment. International Journal of Environmental Health Research 5:223-228. 4. Alvarez-Rodriguez, M. L., L. Lopez-Ocana, J. M. Lopez-Coronado, E. Rodriguez, M. J. Martinez, G. Larriba, and J.-J. R. Coque. 2002. Cork taint of wines: Role of the filamentous fungi isolated from cork in the formation of 2,4,6-trichloroanisole by O methylation of 2,4,6-trichlorophenol. Applied and Environmental Microbiology 68:58605869. 5. Anaissie, E. J., S. L. Stratton, M. C. Dignani, C.-K. Lee, R. C. Summerbell, J. H. Rex, T. P. Monson, and T. J. Walsh. 2003. Pathogenic molds (including Aspergillus species) in hospital water distribution systems: A 3-year prospective study and clinical implications for patients with hematologic malignancies. Blood 101:2542-2546. 6. Birbir, M., O. Ozyaral, C. Johansson, and A. Ilgaz. 1994. Mold strains isolated from unfinished and finished leather goods and shoes. Journal of the American Leather Chemists Association 89:14-19. 7. Bollen, G. J., and M. M. Verf.","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"15 1","pages":"10"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85754162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Reliable PCR amplification from Neurospora crassa genomic DNA obtained from conidia","authors":"S. Henderson, G. Eariss, D. Catcheside","doi":"10.4148/1941-4765.1126","DOIUrl":"https://doi.org/10.4148/1941-4765.1126","url":null,"abstract":"Boil-mediated lysis of Neurospora conidia (Boil-prep) is an extremely rapid, convenient and useful technique to obtain sufficient genomic DNA template for PCR amplification. We routinely use this technique for screening molecular markers, sequencing, and preliminary confirmation of transformants. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This short communications is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol52/iss1/7 24 Fungal Genetics Newsletter Short Communications Reliable PCR amplification from Neurospora crassa genomic DNA obtained from conidia Steven T. Henderson, Graham A. Eariss and David E. A. Catcheside – School of Biological Sciences, Flinders University, Bedford Park, SA 5042, Australia. Fungal Genetics Newsletter 52:24 Boil-mediated lysis of Neurospora conidia (Boil-prep) is an extremely rapid, convenient and useful technique to obtain sufficient genomic DNA template for PCR amplification. We routinely use this technique for screening molecular markers, sequencing, and preliminary confirmation of transformants. However, we have observed periods when successful PCR amplification from Boil-preps has been erratic, hampering the efficacy of this technique. As lysis of conidia results in the liberation of DNA and other cellular components, we reasoned the inconsistent results may have been due to inhibition and/or degradation of the Taq DNA polymerase by cellular material present in the lysis solution. We tried various DNA polymerases and found the reliability of PCR amplification of DNA from Boil-prep template is largely dependant on the specific enzyme used with some Taq polymerases producing <5% successful amplifications. In our hands, Red Hot DNA polymerase from ABgene (cat#AB-0406) facilitates consistent PCR ® amplification (>90%) from Boil-prep template DNA. We routinely use Red Hot DNA polymerase to amplify PCR products up ® to 1.35kb from Boil-prep DNA following the procedure described below (modified from Yeadon and Catcheside 1996). Boil-prep method: A wet loop of 3 7 day old conidia is transferred to 100ìl of sterile Tris-EDTA (pH 8.0) in a 1.5ml microfuge tube and vortexed briefly. The conidial suspension is placed in a boiling waterbath for 10 minutes and then on ice for 5 minutes. The cellular debris is pelleted by centrifugation at 13000 RPM in a benchtop centrifuge for 5 minutes. 70ul of supernatant is transferred to a new 1.5ml microfuge tube and stored at -20oC. 2-5ul of Boil-prep DNA solution is used as a template in a 50ul PCR reaction with 0.5U of Red Hot DNA polymerase. Figure 1. Comparison of PCR amplicons from Boilprep genomic DNA (BP), genomic DNA (G) (prepared by the method of Irelan et al. 1993), plasmid DNA (P) and no DNA (-ve) templates. Size marker (M) = 100bp DNA ladder (New England Biolabs) with a 500bp reference band that contains 97ng of DNA. Each sample lane contains 6ul of a 50ul PCR reaction. DNA yields fro","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"111 1","pages":"24"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80632617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Construction of minimally-sheltered knockouts mutants of Neurospora crassa","authors":"R. L. Metzenberg","doi":"10.4148/1941-4765.1123","DOIUrl":"https://doi.org/10.4148/1941-4765.1123","url":null,"abstract":"I have been developing strains which I think will make it possible to produce \"minimally-sheltered knockouts\" of essential genes routinely. They could equally well be called \"self-adjusting knockdowns\" of essential genes. Such minimally-sheltered knockouts could give results in microarray analysis that would be less subject to artifact than results with heterokaryons or with homokaryons sheltered with an inducible wild-type allele of the gene in question. The method addresses two questions about each cloned gene, henceforth and collectively called \"your favorite gene\" (yfg). yfg should be one which does not pass successfully through a cross as a hygromycin-resistant knockout. (1) Is yfg a dispensable gene? (2) If yfg is found to be essential, what is the \"terminal phenotype\" of the yfgΔ knockout mutation? By \"terminal phenotype,\" I mean that of the heterokaryon between yfgΔ and a partial diploid, yfg+/Δ, in which the partial diploid is the minimum proportion that allows somewhat suboptimal growth. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol52/iss1/4","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"29 1","pages":"11-13"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84711463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Why \"Red bread mold\" is an inappropriate name for Neurospora","authors":"D. D. Perkins","doi":"10.4148/1941-4765.1121","DOIUrl":"https://doi.org/10.4148/1941-4765.1121","url":null,"abstract":"If an organism is already well known to the public before scientists adopt it for research, the popular, vernacular name usually continues to be used. Examples are yeast for Saccharomyces, mouse for Mus, corn for Zea mays, and silkworm for Bombyx. These common n ames are we ll established, and us ers are g enerally comfortable wi th t hem. H owever, some wi dely used r esearch organisms happen to have acquired popular names that are clearly inaccurate or misleading. Such has been the fate of Drosophila and Neurospora. Melvin Green (2002) has protested the use of \"fruit fly\" for Drosophila, pointing out that the name does not apply for the many Drosophila species that use substrates other than fruit, and that it leads to confusion with the Mediterranean fruit fly, a serious agricultural pest. He urges that the popular name fruit fly be abandoned and that the scientific name Drosophila be used in all scientific literature and in textbooks. As with the name fruit fly for Drosophila, objection can be made to using red bread mold for Neurospora. The vernacular name is imprecise and misleading in two respects, regarding both color and substrate. As to color, homothallic Neurospora species are devoid of conidia and of visible carotenoid pigments, while N. crassa and other conidiating species, which do display carotenoids, are orange or yellow-orange rather than red. When dark-grown cultures of a conidiating Neurospora species are first brought into the light, they are colorless. Then, within an hour, they become pigmented. The initial blush of color is pink or red, but this is quickly transformed to orange. The ephemeral red stage, which is rarely seen and is probably unknown to most observers, seems a poor choice for naming the organism. As to substrate, N eurospora exi sted for mi llions of years on nat ural substrates, in t he ab sence of human art ifacts. Calling Neurospora a bread mold might be considered an example of anthropocentric arrogance. Although Neurospora is able to grow profusely on bread, and was first recorded as a nuisance in bakeries (Payen 1843; see Perkins 1991), its occurrence is rare compared to other contaminating molds, especially since antifungal agents were introduced and bakery sanitation was improved. I have myself seen many examples of moldy bread that was black or green, but never one that was orange or red.","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"2 1","pages":"7-8"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74388365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of gpd genes and their protein products in basidiomycetes","authors":"Sreedhar Kilaru, U. Kües","doi":"10.4148/1941-4765.1125","DOIUrl":"https://doi.org/10.4148/1941-4765.1125","url":null,"abstract":"We compared promoters, coding sequences, introns and terminators of glyceraldehyde 3-phosphate dehydrogenase genes (gpd) from various basidiomycetes. Coding regions of these housekeeping genes are highly conserved (between 60 to 99% DNA identity) whilst non-coding regions have DNA identities of around 40%. Amongst all homobasidiomycete promoters, the TATA region and a CT-rich region with the potential transcription start sites are highest conserved. Surprisingly, there are no other conserved motifs common to all promoters. Up to five introns are clustered at the far 5 ́ ends of the genes, hinting to a potential function in efficient gene expression. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol52/iss1/6 18 Fungal Genetics Newsletter Comparison of gpd genes and their protein products in basidiomycetes Sreedhar Kilaru and Ursula Kües Molecular Wood Biotechnology, Institute of Forest Botany, Georg-August-University Göttingen, 37077 Göttingen, Germany Fungal Genetics Newsletter 52:18-23 We compared promoters, coding sequences, introns and terminators of glyceraldehyde 3-phosphate dehydrogenase genes (gpd) from various basidiomycetes. Coding regions of these housekeeping genes are highly conserved (between 60 to 99% DNA identity) whilst non-coding regions have DNA identities of around 40%. Amongst all homobasidiomycete promoters, the TATA region and a CT-rich region with the potential transcription start sites are highest conserved. Surprisingly, there are no other conserved motifs common to all promoters. Up to five introns are clustered at the far 5 ́ ends of the genes, hinting to a potential function in efficient gene expression. Little is known about promoters in higher basidiomycetes. Constitutive promoter activities have been described for some homologous and heterologous promoters in Coprinopsis cinerea with the Agaricus bisporus gpdII (glyceraldehyde 3-phosphate dehydrogenase gene 2) promoter being highest in activity (Kilaru et al., 2005). Use of gpd promoters from A. bisporus, Flammulina velutipes, Lentinula edodes, Phanerochaete chrysosporium, Schizophyllum commune and Trametes versicolor has by now been made in different species either for laccase and peroxidase production or for expression of gfp (green fluorescent protein gene) or the bacterial hygromycin resistance gene hph (for references see Kilaru et al., 2005). Surprisingly, homology among these promoter sequences is relatively low (Kilaru et al., 2005). In contrast, the two known gpd genes from A. bisporus (termed gpdI and gpdII), the single gpd gene from P. chrysosporium and an isolated gpd gene from S. commune have been described as highly conserved in intron positions as well as in sequence of their products (Harmsen et al., 1992). Analysis of all gpd genes from basidiomycetes currently present in the NCBI database and of t","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"6 1","pages":"18-23"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74385843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Expression and Visualization of Red Fluorescent Protein (RFP) in Neurospora crassa","authors":"M. Freitag, E. Selker","doi":"10.4148/1941-4765.1124","DOIUrl":"https://doi.org/10.4148/1941-4765.1124","url":null,"abstract":"We report the expression of Discosoma red fluorescent protein (RFP) and RFP fusion proteins in Neurospora crassa. RFP was expressed under the control of the Neurospora ccg-1 promoter in transformants with single copies integrated at the his-3 locus by gene targeting. Because this RFP gene, tdimer2(12), contains a 677 bp direct tandem repeat of dsRed, RFP constructs underwent RIP at high frequency in rid strains. Fusion proteins of RFP to the amino terminus of Neurospora heterochromatin protein 1 (HP1) +","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"25 1","pages":"14-17"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76813655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Using Neurospora to demonstrate the unidirectional nature of fungal mating.","authors":"K. McCluskey","doi":"10.4148/1941-4765.1122","DOIUrl":"https://doi.org/10.4148/1941-4765.1122","url":null,"abstract":"","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"76 1","pages":"9-10"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83367969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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