粗神经孢子虫红色荧光蛋白(RFP)的表达与可视化

Fungal Genetics Reports Pub Date : 2005-12-01 DOI:10.4148/1941-4765.1124

M. Freitag, E. Selker

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引用次数: 37

摘要

我们报道了粗神经孢子虫中dissoma红色荧光蛋白(RFP)和RFP融合蛋白的表达。RFP在神经孢子菌ccg-1启动子的控制下，在his-3位点通过基因靶向整合的单拷贝转化子中表达。由于该RFP基因tdimer2(12)含有677 bp的dsRed直接串联重复序列，因此RFP构建体在rid菌株中被RIP的频率很高。RFP与神经孢子虫异染色质蛋白1 (HP1)氨基末端的融合蛋白

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Expression and Visualization of Red Fluorescent Protein (RFP) in Neurospora crassa

We report the expression of Discosoma red fluorescent protein (RFP) and RFP fusion proteins in Neurospora crassa. RFP was expressed under the control of the Neurospora ccg-1 promoter in transformants with single copies integrated at the his-3 locus by gene targeting. Because this RFP gene, tdimer2(12), contains a 677 bp direct tandem repeat of dsRed, RFP constructs underwent RIP at high frequency in rid strains. Fusion proteins of RFP to the amino terminus of Neurospora heterochromatin protein 1 (HP1) +

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Fungal Genetics Reports

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