Fungal Genetics Reports最新文献

{"title":"No evidence for intra-allelic complementation at the osmotic-1 locus of Neurospora crassa","authors":"C. Selitrennikoff, Shelly J Wilson, S. Renault, R. O’Rourke","doi":"10.4148/1941-4765.1151","DOIUrl":"https://doi.org/10.4148/1941-4765.1151","url":null,"abstract":"The osmotic-1 (os-1) locus of Neurospora crassa encodes a protein with homology to bacterial and plant two-component histidine kinases. The os-1 protein appears to be an osmo-sensor and is the first step in a MAP kinase cascade that regulates intra-cellular osmolarity and cell-wall synthesis (Alex L, Borkovich K, Simon MI. Hyphal development in Neurospora crassa: involvement of a two-component histidine kinase Proc Natl Acad Sci U S A. 93:3416-21. 1996; Schumacher M, Enderlin C, Selitrennikoff CP. The osmotic-1 locus of Neurospora crassa encodes a putative histidine kinase similar to osmosensors of bacteria and yeast. Curr Microbiol. 34:340-7. 1997). Mutants defective at the os-1 locus are sensitive to a number of hyper-osmotic conditions, including 4% NaCl (Mehadevan P. and Tatum E. Relationship of the major constituents of the Neurospora crassa cell wall to wild-type and colonial morphology. J. Bacteriol. 90:1073-1081. 1965). In other organisms, e.g., prokaryotes, similar histidine kinases exist as homodimers (C. Tomomori C, Tanaka T, Dutta R, et al. Solution structure of the homodimeric core domain of Escherichia coli histidine kinase EnvZ Nat. Struct. Biol. 6:729. 1999) in which an extracellular signal induces the autophosphorylation of a histidyl residue of one member of the dimer. The phosphoryl group is subsequently transferred to an aspartyl residue of the other dimer pair, triggering a regulatory kinase cascade.","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"110 1","pages":"12-13"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79246297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurospora tetrasperma bibliography - Additions","authors":"D. D. Perkins","doi":"10.4148/1941-4765.1160","DOIUrl":"https://doi.org/10.4148/1941-4765.1160","url":null,"abstract":"During the evolutionary divergence of N. tetrasperma from the eight-spored Neurospora species, ascus development was reprogrammed with the result that each of the four large ascospores is heterokaryotic, containing nuclei of both mating types, and germlings are self-fertile. Unique features of genome oganization, cell biology, and population structure have attracted investigators to use this pseudohomothallic, four-spored species for a wide range of studies. A bibliography listing 164 publications was published in 1994 in Fungal Genetics Newsletter 41:72-78. Eighty-six additional publications are listed here. In addition to recent papers, these include some theses, abstracts, and papers that were omitted from the previous list. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This special paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol50/iss1/14 24 Fungal Genetics Newsletter Neurospora tetrasperm a bibliography – Additions David D. Perkins Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020. Fungal Genet. Newsl. 50:24-26 During the evolutionary divergence of N. tetrasperma from the eight-spored Neurospora species, ascus development was reprogrammed with the result that each of the four large ascospores is heterokaryotic, containing nuclei of both mating types, and germlings are self-fertile. Unique features of genome oganization, cell biology, and population structure have attracted investigators to use this pseudohomothallic, four-spored species for a wide range of studies. A bibliography listing 164 publications was published in 1994 in Fungal Genetics.Newsletter 41:72-78. Eighty-six additional publications are listed here. In addition to recent papers, these include some theses, abstracts, and papers that were omitted from the previous list. Adhvaryu K.K., and R. Maheshwari. 2002. Heterogeneity in NTS of rDNA in localized populations of Neurospora. Curr. Sci. 82:1015-1020. Agarwal, C. P., and R. K. S. Chauhan. 1976. Neurospora tetrasperma: New record of its occurrence in Indian soil and its cellulolytic activity. Proc. Indian Nat. Sci. Acad., Part B: Biol. Sci. 42:122-124. Ardizzi, J. P., and A. M. Srb. 1981 . “E-like” ascospore excision mutants in Neurospora tetrasperma resistant to either p-DLfluorophenylalanine or methyl benzimidazol-2-yl carbamate. Neurospora Newslett. 28:6. Arganoza, M. T., J. Min, Z. Hu, and R. A. Akins. 1994. Distribution of seven homology groups of mitochondrial plasmids in Neurospora: Evidence for widespread mobility between species in nature. Curr. Genet. 26:62-73. Attoh, G. T . 1986. Ribosomal DN A systematics of homothallic species of Neurospora: A phylogenetic analysis. Ph.D. thesis, Howard University: Diss. Abstr. Intl. 47-10B:4060. Belmans, D. L., A. J. van Laere, and J. A. van Assche. 1983. Effect of n-alcohols and high pressure on the heat activation of Neurospora tetrasperma ascospores. Ar","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"1 1","pages":"24-26"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89413144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A GATEWAY™ Destination Vector For High-Throughput Construction of Neurospora crassa histidine-3 Gene Replacement Plasmids","authors":"J. Haag, D. W. Lee, R. Aramayo","doi":"10.4148/1941-4765.1149","DOIUrl":"https://doi.org/10.4148/1941-4765.1149","url":null,"abstract":"We report the construction of a Destination Vector, called pJHAM007, for the targeted integration of DNA sequences at the histidine-3 (his-3) locus of Neurospora crassa. pJHAM007 has all the necessary features required to perform a simple, rapid and efficient GATEWAYTM recombinational cloning with an Entry Clone to yield a his-3-gene replacement Destination Vector. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol50/iss1/3 6 Fungal Genetics Newsletter A GATEWAYTM Destination Vector For High-Throughput Construction of Neurospora crassa histidine-3 Gene Replacement Plasmids Haag, Jeremy R., Lee, Dong, W ., and Aramayo, Rodolfo . Department of Biology, Washington University, Campus Box 1137, 1 Brookings Drive, St. Louis, M O 63130. Department of Biology, Texas A&M U niversity, Room 415 , Building BSBW , College Station, TX 77843-3258 We report the construction of a Destination Vector, called pJHAM007, for the targeted integration of DNA sequences at the histidine-3 (his-3) locus of Neurospora crassa . pJHAM007 has all the necessary features required to perform a simple, rapid and efficient GATEW AYTM recombinational cloning with an Entry Clone to yield a his-3-gene replacement Destination Vector. Fungal Genet Newsl 50:6-8 Gene replacement is a powerful tool to construct isogenic strains containing different DNA sequences integrated at the same chromosomal position. The most popular locus used for gene targeting in Neurospora crassa is of the metabolic gene histidine-3 (his-3). Several generations of plasmids for integration at this chromosomal position have been constructed (Sachs and Ebbole 1990 Fungal Genet. Newsl. 37: 35-36, Ebbole 1990 Fungal Genet. Newsl. 37: 15, M argolin, et al. 1997 Fungal Genet. Newsl. 44: 34-36, Aramayo and Metzenberg 1996 Fungal Genet. Newsl. 43: 9-13). Recently, we described the construction of a new set of N. crassa strains and plasmids that represent a significant improvement over previous systems because they allow the investigator to screen in one simple step for homokaryotic transformants containing the insertion of a test sequence among a population of primary histidine-independent transformants (Lee, et al. 2003 Curr. Genet. DOI 10.1007/s00294-002-0366-z). These new tools have significantly reduced the time it takes to construct new N. crassa strains. To expedite this system even further, we have designed and constructed a new plasmid, pJHAM 007, that can be used for the high-throughput cloning of DNA inserts, to generate his-3-gene replacement plasmids for d ifferent types of largeor smallscale genome analysis. Plasmid pJHAM007 is based on the GATEWAYTM system (Walhout, et al. 2000 Method . Enzymol. 328: 575-592). GATEWAYTM is a novel universal system for cloning and subcloning DNA sequences that uses phage lambda (8)-based sitespecific recombination (Landy 1989","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"08 1","pages":"6-8"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83320797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wacslaw Gajewski, 1911-1997","authors":"D. D. Perkins","doi":"10.4148/1941-4765.1156","DOIUrl":"https://doi.org/10.4148/1941-4765.1156","url":null,"abstract":"Wacslaw Gajewski, 1911-1997 Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This obituary is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol50/iss1/10","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"50 1","pages":"21-21"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88610128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helga Ninnemann, 1938-2003","authors":"J. Maier, Stuart Brody","doi":"10.4148/1941-4765.1159","DOIUrl":"https://doi.org/10.4148/1941-4765.1159","url":null,"abstract":"Helga Ninnemann, 1938-2003 Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This obituary is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol50/iss1/13","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"3 1","pages":"23-23"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73997624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alexandra Putrament, 1926-2003","authors":"D. D. Perkins","doi":"10.4148/1941-4765.1157","DOIUrl":"https://doi.org/10.4148/1941-4765.1157","url":null,"abstract":"Alexandra Putrament, 1926-2003 Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This obituary is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol50/iss1/11","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"31 1","pages":"21-21"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87901492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Raymond W. Barratt, 1920-2002","authors":"D. Newmeyer, D. D. Perkins","doi":"10.4148/1941-4765.1155","DOIUrl":"https://doi.org/10.4148/1941-4765.1155","url":null,"abstract":"Raymond W. Barratt 1920-2002 Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This obituary is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol50/iss1/9 20 Fungal Genetics Newsletter Obituaries Raymond W. Barratt 1920-2002 Raymond Barratt, who died of cancer in December, 2002, was a prominent player in the early development of fungal genetics. After early work with fungal plant pathogens at the Connecticut Agricultural Experiment Station, he switched to Neurospora and became Ed Tatum's first graduate student at Yale. When the Tatum lab moved to Stanford in 1948, Ray continued as a Research Fellow, conducting his own research, supervising the laboratory, and becoming a teacher, helper, and friend to all the new students and postdocs. During this period he took the initiative in assigning gene names and formulating rules of genetic nomenclature for Neurospora (1) and in bringing together genetic and phenotypic information on all the known genes into what might be called the first Neurospora compendium, which included the first comprehensive maps (2). In 1954 he went to Dartmouth as a faculty member. He organized the Fungal Genetics Stock Center (FGSC), gathering Neurospora mutant and wild-type strains, obtaining funding from NSF, perfecting preservation methods, and periodically publishing stock lists in the Neurospora Newsletter (now Fungal Genetics Newsletter). The Newsletter, produced and distributed by FGSC, was founded with Ray's help in 1961 following the first Neurospora Information Conference (now Fungal Genetics Conference), of which he was a co-organizer. He continued to direct the stock center for 25 years, during which it was expanded to include other filamentous fungi. In 1970 he resigned as chair of the Biology Department at Dartmouth and became Professor of Biology and Dean of Sciences at California State University, Humboldt, taking the stock center with him. Ray's research, though limited, contributed significantly to progress at the time. From studies of morphological mutants (3) and chemical mutagens, he turned to gene-enzyme relations. He was fascinated with mutants having complex metabolic effects; for example, phe-1 (4), ilv (5), and am (6). He was attracted to am mutants because their growth requirement could be satisfied by any of numerous amino acids, and his most extensive studies were of the am gene, which specifies NADP-specific glutamate dehydrogenase. (Stud ies of am were begun independently and carried to culmination by John Fincham and his colleagues.) After his move to Dartmouth, Ray's experimental contributions were limited by other responsibilities. He was a superb organizer, and the choice to direct the stock center and to assume various academic obligations at the expense of his research was no doubt made deliberately. The stock center was set up and run fastidiously, with help of the curator, Bill Ogata, who followed Ray from St","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"60 1","pages":"20-20"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88015755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal Genetics Stock Center Catalogue of Strains, 10th Edition","authors":"K. McCluskey, M. Plamann","doi":"10.4148/1941-4765.1165","DOIUrl":"https://doi.org/10.4148/1941-4765.1165","url":null,"abstract":"Catalogue of Strains, 10th edition, 2004, supplement to Fungal Genetics Newsletter No. 51. This catalogue contains lists of materials held by the Fungal Genetics Stock Center. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This supplementary material is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/17 FUNGAL GENETICS STOCK CENTER CATALOGUE OF STRAINS","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"37 1","pages":"14"},"PeriodicalIF":0.0,"publicationDate":"2002-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85902954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrections to the Neurospora Compendium","authors":"D. D. Perkins","doi":"10.4148/1941-4765.1190","DOIUrl":"https://doi.org/10.4148/1941-4765.1190","url":null,"abstract":"Corrections to the Neurospora Compendium Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This brief note is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol49/iss1/8","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"30 1","pages":"17"},"PeriodicalIF":0.0,"publicationDate":"2002-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86879232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved selection for inositol-utilization following transformation of Neurospora crassa","authors":"R. L. Metzenberg","doi":"10.4148/1941-4765.1191","DOIUrl":"https://doi.org/10.4148/1941-4765.1191","url":null,"abstract":"Plasmids based on the cloned inositol gene of Neurospora crassa are potentially very useful as a transformation marker, or for techniques like insertional mutagenesis. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This brief note is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol49/iss1/9 18 Fungal Genetics Newsletter Improved selection for inositol-utilization following transformation of Neurospora crassa Robert L. Metzenberg Department of Biological Sciences, Stanford U niversity, Stanford , California 94305-5020; present address, Dept. of Chemistry and Biochemistry, University of California, Los Angeles CA 90095-1569 Plasmids based on the cloned inositol gene of Neurospora crassa are potentially very useful as a transformation marker, or for techniques like insertional mutagenesis. Transformation is generally done on spheroplasts stabilized with sorbitol, or by electroporation of conidia, also suspended in sorb itol. For transformation to inositol independence, however, this gives an unacceptable background of nontransformants that grow on the nominally inositol-free medium. It appears that sorbitol always contains a trace of inositol that cannot be completely removed by recrystallization. It seems possible that sorbitol, which differs from inositol only by a pair of hydrogens, spontaneously generates the latter by air oxidation. Substituting a-methyl glucoside for the sorbitol on an equimolar basis can circumvent this problem. a-Methyl glucoside works well as an osmoticum, and gives clean backgrounds in transformation of inl mutants to inositol independence. Published by New Prairie Press, 2017","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"89 1","pages":"18"},"PeriodicalIF":0.0,"publicationDate":"2002-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89061477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}