同源长度对粗神经孢子虫基因断裂的影响

Fungal Genetics Reports Pub Date : 2005-12-01 DOI:10.4148/1941-4765.1120

Y. Nakanishi, C. Ishii, H. Inoue

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引用次数: 1

摘要

对于野生型粗神经孢子虫的靶向基因破坏，用于破坏的盒体两侧有1000 bp的同源序列足以产生10%以上的同源重组。我们在这里报道，不同的同源长度在每一边似乎有不同的影响同源重组频率。本作品采用知识共享署名-相同方式共享4.0许可协议。这篇常规论文可在真菌遗传学报告:http://newprairiepress.org/fgr/vol52/iss1/1

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An effect of homology length on gene disruption in Neurospora crassa

For targeted gene disruption in wild-type Neurospora crassa, 1000-bp of homologous sequences on either side of the cassette used for disruption is sufficient to give more than 10 % homologous recombination. We report here that varying the length of homology on each side seems to have different effects on the homologous recombination frequency. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol52/iss1/1

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Fungal Genetics Reports

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