Frontiers in Physiology最新文献

{"title":"Exercise training reduces cardiac fibrosis, promoting improvement in arrhythmias and cardiac dysfunction in an experimental model of chronic chagasic cardiomyopathy.","authors":"Alex Cleber Improta-Caria, Carolina Kymie Vasques Nonaka, Pâmela Santana Daltro, Carine Machado Azevedo, Breno Cardim Barreto, Gisele Batista Carvalho, Juliana Fraga Vasconcelos, Bruno Solano de Freitas Souza, Simone Garcia Macambira, Milena Botelho Pereira Soares","doi":"10.3389/fphys.2025.1558678","DOIUrl":"https://doi.org/10.3389/fphys.2025.1558678","url":null,"abstract":"<p><strong>Background: </strong>Chagas disease, caused by the parasite <i>Trypanosoma cruzi</i>, is associated with inflammation and fibrosis, which characterizes chronic Chagasic cardiomyopathy (CCC). CCC manifests as arrhythmias, hypertrophy or dilation of the left ventricle, and it may progress to heart failure. Therefore, interventions are needed to slow the progression of CCC. Aims: We investigated the effects of exercise training in an animal model of CCC.</p><p><strong>Methods: </strong>C57BL/6 mice infected with <i>Trypanosoma cruzi</i> were submitted to a progressively treadmill exercise training protocol. The cardiac function was evaluated by echocardiogram and electrocardiogram. RT-qPCR and morphometric analyses were performed on samples of cardiac tissue to quantify inflammation and fibrosis.</p><p><strong>Results: </strong>EKG analysis confirmed that all infected mice developed arrhythmias, with different degrees of severity. Exercise improved arrhythmias in 43.75% of chagasic trained mice, and the remaining mice did not show any alteration in EKG. The untrained chagasic group had no improvement in arrhythmias. The ventricular compliance in chagasic trained mice increased, as revealed by the reduction in isovolumetric relaxation time when compared to untrained mice. Exercise induced the reduction of gene expression of TGF-β, TNF-α, IL-1β, IL-6 and MMP-9 and reduced fibrosis in the heart tissue of chagasic mice.</p><p><strong>Conclusion: </strong>Exercise reduced fibrosis in the heart and skeletal muscle, favoring the improvement of arrhythmias, and augment of cardiac complacency in mice with CCC, in addition to decreasing the expression of profibrotic and proinflammatory genes in the heart.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1558678"},"PeriodicalIF":3.2,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Ex vivo</i> quantification of intracellular pH in <i>Drosophila</i> Malpighian tubule reveals basolateral HCO₃ ^-/oxalate exchange through a novel oxalate transporter \"Neat\".","authors":"Adam J Rossano, Lili Zhang, Jacob B Anderson, Heather L Holmes, Asim K Mandal, James W Decker, David B Mount, Michael F Romero","doi":"10.3389/fphys.2025.1468451","DOIUrl":"https://doi.org/10.3389/fphys.2025.1468451","url":null,"abstract":"<p><strong>Introduction: </strong>Nephrolithiasis is a painful and costly healthcare complication. The most common kidney stones are composed of calcium oxalate and thus renal handling of oxalate is an important facet of understanding the pathogenesis of nephrolithiasis. Recently, the <i>Drosophila melanogaster</i> Malpighian tubule (MT) has emerged as a robust model of trans-epithelial ion transport and nephrolithiasis as MTs readily form luminal calcium-oxalate crystals in the presence of oxalate. <i>Drosophila</i> Prestin (dPrestin, Slc26a6) transports oxalate across the apical surface of the MT into the lumen but a full model of the trans-epithelial movement of oxalate (Ox^2-) in the <i>Drosophila</i> MT has been lacking as the basolateral oxalate transporter has remained uncharacterized.</p><p><strong>Methods: </strong>The objective of this work was to identify and characterize the <i>Drosophila</i> basolateral Ox^2- transporter through <i>ex vivo</i> real-time quantification of intracellular pH (pH_i) and <i>Xenopus</i> oocyte transport assays.</p><p><strong>Results: </strong>A putative basolateral oxalate transporter CG5002 (\"Neat\") was identified through sequence homology and displayed robust Cl^--independent Ox^2- transport and electroneutral Ox^2- transport in <i>Xenopus</i> oocytes. pH_i in extracted fly MTs was monitored by using the GAL4/UAS system to selectively express pHerry, a pseudo-ratiometric genetically-encoded pH indicator (GEpHI) in the cytosol of the principal cells of the MT. Basolateral perfusion of MTs in CO₂/HCO₃ ^--buffered solution produced a large acidification followed by rapid recovery in the transitional segment of the anterior MT. Recovery was interrupted by basolateral application of 1 mM Ox^2- or 1 mM SO₄ ². Tissue specific knock-down of Neat with interference RNA (RNAi) reduced the rate of acid-loading in the transitional segment of the MT with regard to Ox^2- and SO₄ ^2-. Knockdown of Neat in the MT also significantly reduced luminal calcium oxalate crystal formation in a fly <i>ex vivo</i> model of calcium oxalate nephrolithiasis.</p><p><strong>Discussion: </strong>These data indicate Neat is a significant <i>Drosophila</i> basolateral MT oxalate transporter and the basolateral movement of oxalate is functionally coupled to movement of acid equivalents, potentially as Ox^2-/HCO₃ ^- exchange, Ox^2-/OH^- exchange, or Ox^2-:H⁺ co-transport.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1468451"},"PeriodicalIF":3.2,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age- and sex-specific reference values for CT-based low skeletal muscle quantity and quality in healthy living kidney donors.","authors":"David Martin, Paola Dolce, Martin Hübner, Tobias Zingg, David Fuks, Jean-Pierre Venetz, Damien Maier, Maurice Matter, Fabio Becce","doi":"10.3389/fphys.2025.1566463","DOIUrl":"https://doi.org/10.3389/fphys.2025.1566463","url":null,"abstract":"<p><strong>Background: </strong>Computed tomography (CT) imaging is a useful tool for assessing skeletal muscle mass and quality. The present study aimed to determine age- and sex-specific reference values for CT-based skeletal muscle markers in a healthy population, and to correlate them with serum creatinine and 24-h urinary creatinine excretion (24h-UCE).</p><p><strong>Methods: </strong>Skeletal muscle index (SMI) - a marker of muscle mass/quantity - and skeletal muscle radiation attenuation (SMRA) and intermuscular adipose tissue index (IMATI) - markers of muscle quality/myosteatosis - were determined using a deep-learning-based method from axial CT images at the level of the 3^rd lumbar vertebra in living kidney donors assessed between 01/2005 and 05/2023. Age- and sex-specific reference values were determined by the 5^th percentile, and correlation was tested with the Pearson correlation coefficient.</p><p><strong>Results: </strong>CT scans of 394 healthy individuals were included. The mean age was 53 years (SD 12), mean BMI was 25.2 kg/m² (SD 3.9), and 130 patients (33%) were male. The reference values for low skeletal muscle mass (SMI) in males were 43.7 cm²/m² (20-39 years), 44.9 cm²/m² (40-59 years), and 39.7 cm²/m² (≥60 years). In females, the corresponding values were 33.8 cm²/m² (20-39 years), 34.8 cm²/m² (40-59 years), and 31.2 cm²/m² (≥60 years). SMI showed a moderate correlation with serum creatinine (r = 0.452, p < 0.001) but a weak correlation with 24h-UCE (r = 0.188, p = 0.003). Correlations were all weak for SMRA (creatinine: r = 0.220, p < 0.001; 24h-UCE: r = 0.177, p = 0.006) and IMATI (creatinine: r = -0.101, p = 0.054; 24h-UCE, r = -0.108, p = 0.093).</p><p><strong>Conclusion: </strong>The age- and sex-specific reference values reported here could be used in clinical practice and future studies to identify patients at risk of muscle decline.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1566463"},"PeriodicalIF":3.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in <i>Megalurothrips usitatus</i> (thysanoptera: thripidae).","authors":"Qingfang Hou, Linlin Yuan, Haifeng Jin, Han Yan, Fen Li, Shaoying Wu","doi":"10.3389/fphys.2025.1612671","DOIUrl":"https://doi.org/10.3389/fphys.2025.1612671","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fphys.2023.1161680.].</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1612671"},"PeriodicalIF":3.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantifying ventilatory control with 3% CO2 inhalation during exercise.","authors":"Suhaib M Hashem, Stanley M Yamashiro, Takahide Kato, Takaaki Matsumoto, Vasilis Z Marmarelis","doi":"10.3389/fphys.2025.1528519","DOIUrl":"https://doi.org/10.3389/fphys.2025.1528519","url":null,"abstract":"<p><strong>Introduction: </strong>CO2 mediated ventilation is mainly controlled by two homeostatic mechanisms. The central chemoreceptors are slower mechanisms that focus on blood pH sensing in the brain stem while the peripheral chemoreceptors are quicker to respond and reside in the carotid bodies. Quantification of these mechanisms in humans remain debated.</p><p><strong>Objective: </strong>To quantify the impact that the central and peripheral chemoreceptors have on ventilation in response to changes in PETCO2 during exercise with normoxic breathing and 3% CO2 inhalation.</p><p><strong>Method: </strong>Six healthy males participated in a 5-stage bike protocol with and without 3% CO2 inhalation. We analyzed the time series data of their breath-by-breath PETCO2 and ventilation and generated a one input-one output model via the Laguerre expansion technique (LET) to construct the gain function and quantify the low (0.002-0.029 Hz) and high (0.03-0.15 Hz) frequency components using the weighted gain averages (WGA) as estimators of central and peripheral chemoreflex mechanisms respectively.</p><p><strong>Results: </strong>3% CO2 inhalation caused a significant increase the high frequency WGAs at rest and in all levels of exercise except heavy exercise. The low frequency WGAs, however, only maintain significance during rest and the baseline session of exercise.</p><p><strong>Conclusion: </strong>Changes in WGA can be used as quantitative estimates of central and peripheral chemoreflexes. 3% CO2 activates both reflexes and is more apparent in the higher frequency WGAs during exercise due to the oxygen dependent mechanisms effects of exercise.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1528519"},"PeriodicalIF":3.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From function to structure: how myofibrillogenesis influences the transverse-axial tubular system development and its peculiarities.","authors":"Zuzana Sevcikova Tomaskova, Katarina Mackova","doi":"10.3389/fphys.2025.1576133","DOIUrl":"https://doi.org/10.3389/fphys.2025.1576133","url":null,"abstract":"<p><p>The transverse-axial tubular system (TATS) is the extension of sarcolemma growing to the cell interior, providing sufficient calcium signaling to induce calcium release from sarcoplasmic reticulum cisternae and stimulate the contraction of neighboring myofibrils. Interestingly, the development of TATS is delayed and matures during the <i>post-partum</i> period. It starts with small invaginations near the sarcolemma, proceeding to grow an irregular network that is later assembled into the notably transversally oriented tubular network. Accumulating evidence supports the idea that the development of TATS is linked to cell dimensions, calcium signaling, and increasing myofibrillar content orchestrated by electromechanical stimulation. However, the overall mechanism has not yet been described. The topic of this review is the development of TATS with an emphasis on the irregular phase of tubule growth. The traditional models of BIN1-related tubulation are also discussed. We summarized the recently described protein interactions during TATS development, mainly mediated by costameric and sarcomeric proteins, supporting the idea of the coupling sites between TATS and the myofibrils. We hypothesize that the formation and final organization of the tubular system is driven by the simultaneous development of the contractile apparatus under cycling electromechanical stimulus.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1576133"},"PeriodicalIF":3.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TongueNet: a multi-modal fusion and multi-label classification model for traditional Chinese Medicine tongue diagnosis.","authors":"Lijuan Yang, Qiumei Dong, Da Lin, Xinliang Lü","doi":"10.3389/fphys.2025.1527751","DOIUrl":"https://doi.org/10.3389/fphys.2025.1527751","url":null,"abstract":"<p><p>Tongue diagnosis in Traditional Chinese Medicine (TCM) plays a crucial role in clinical practice. By observing the shape, color, and coating of the tongue, practitioners can assist in determining the nature and location of a disease. However, the field of tongue diagnosis currently faces challenges such as data scarcity and a lack of efficient multimodal diagnostic models, making it difficult to fully align with TCM theories and clinical needs. Additionally, existing methods generally lack multi-label classification capabilities, making it challenging to simultaneously meet the multidimensional requirements of TCM diagnosis for disease nature and location. To address these issues, this paper proposes TongueNet, a multimodal deep learning model that integrates tongue image data with text-based features. The model utilizes a Hierarchical Aggregation Network (HAN) and a Feature Space Projection Module to efficiently extract and fuse features while introducing consistency and complementarity constraints to optimize multimodal information fusion. Furthermore, the model incorporates a multi-scale attention mechanism (EMA) to enhance the diversity and accuracy of feature weighting and employs a Kolmogorov-Arnold Network (KAN) instead of traditional MLPs for output optimization, thereby improving the representation of complex features. For model training, this study integrates three publicly available tongue image datasets from the Roboflow platform and enlists multiple experts for multimodal annotation, incorporating multi-label information on disease nature and location to align with TCM clinical needs. Experimental results demonstrate that TongueNet outperforms existing models in both disease nature and disease location classification tasks. Specifically, in the disease nature classification task, it achieves 89.12% accuracy and an AUC of 83%; in the disease location classification task, it achieves 86.47% accuracy and an AUC of 81%. Moreover, TongueNet contains only 32.1 M parameters, significantly reducing computational resource requirements while maintaining high diagnostic performance. TongueNet provides a new approach for the intelligent development of TCM tongue diagnosis.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1527751"},"PeriodicalIF":3.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics and hemolysis analysis of centrifugal blood pumps under different speed modulations.","authors":"Chengxuan Su, Donghai Jin, Guangmao Liu, Shulei Li, Xingmin Gui","doi":"10.3389/fphys.2025.1575971","DOIUrl":"https://doi.org/10.3389/fphys.2025.1575971","url":null,"abstract":"<p><strong>Introduction: </strong>Constant-speed methods are widely applied and studied in rotary blood pumps. However, various speed modulation which have been used in commercial blood pump lacks validation of the ventricular assist capability and hemolysis potential.</p><p><strong>Methods: </strong>This study investigates the hydrodynamic performance and hemolysis of a rotary ventricular assist device under sinusoidal speed modulation, focusing on the combined effects of phase, baseline speed, and speed fluctuation amplitude.</p><p><strong>Results: </strong>Computational fluid dynamics (CFD) coupled with a dynamic cardiovascular model revealed that counter-phase modulation reduces hemolysis index (HI) fluctuations compared to in-phase conditions, while higher baseline speeds increase time-averaged HI due to prolonged exposure to non-physiological shear stress. Larger amplitudes expand the operational range but exacerbate HI variability.</p><p><strong>Discussion: </strong>These findings demonstrate that phase synchronization critically balances pulsatility and hemocompatibility, providing actionable insights for adaptive speed control strategies in clinical practice.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1575971"},"PeriodicalIF":3.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of <i>in ovo</i>-fed amino acids on muscle and liver metabolome of broiler chickens at 24 h post-hatch.","authors":"Moustafa Yehia, Angel Rene Alfonso-Avila, Jean-Michel Allard Prus, Véronique Ouellet, Nabeel Alnahhas","doi":"10.3389/fphys.2025.1542426","DOIUrl":"https://doi.org/10.3389/fphys.2025.1542426","url":null,"abstract":"<p><p><i>In ovo</i> administration of amino acids has been shown to alleviate the adverse effects of heat stress on broiler chickens during the finisher phase. However, their specific influence on thermogenic organs in the early post-hatch period is not fully understood. Therefore, the aim of the present study was to explore and investigate the effects of <i>in ovo</i>-fed amino acids on amino acid metabolism in the liver and muscle of one-day-old broiler chicks. To achieve this, breast muscle and liver samples were taken from six randomly selected chicks per experimental group and subjected to a targeted metabolomic analysis. The experimental groups included a control group injected with 52 µL of sterile diluent/egg (CTRL), a group injected with 3.0 mg of L-Met + 2.0 mg of L-Cys/egg (T1), and a group injected with 0.4 mg of L-Leu + 1.6 mg of L-Met + 1.6 mg of L-Cys/egg (T2). The Sparse Partial Least Square - Discriminant Analysis (sPLS-DA) showed that T1 and T2 had very similar metabolomic profiles. Consequently, data from T1 and T2 were merged into a single group (Injected) for statistical analysis. Compared to CTRL, multiple pathways were significantly enriched in the muscle and liver of the Injected group. These enriched pathways included those involved in the metabolism of cysteine and methionine (FDR = 0.01), glutathione (FDR < 0.001), histidine (FDR = 0.01), taurine (FDR = 0.01), glycine, serine, and threonine (FDR = 0.01) as well as the pathway of arginine biosynthesis (FDR = 0.03). Moreover, only four muscle metabolites: homocysteine (r = -0.63, P = 0.03), S-Adenosyl-homocysteine (r = -0.62, P = 0.03), phosphocholine (r = 0.50, P = 0.01), and betaine (r = 0.52, P = 0.004), as well as four liver metabolites: phenyl pyruvic acid (r = 0.55, P = 0.02), dimethylglycine (r = 0.55, P = 0.03), phenylalanine (r = 0.50, P = 0.02), and alpha-aminobutyric acid (r = -0.53, P = 0.02) were significantly correlated with the rectal temperature of sampled chicks, suggesting a role of these metabolites in thermoregulation. In conclusion, the <i>in ovo</i> feeding of amino acids on embryonic day 18 was associated with the enrichment of pathways directly or indirectly involved in the response of the antioxidant defense system to oxidative stress in the liver and muscle tissues.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1542426"},"PeriodicalIF":3.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reconstruction of 12-lead ECG: a review of algorithms.","authors":"Ekenedirichukwu N Obianom, G André Ng, Xin Li","doi":"10.3389/fphys.2025.1532284","DOIUrl":"https://doi.org/10.3389/fphys.2025.1532284","url":null,"abstract":"<p><strong>Purpose: </strong>This paper aims to review the literature on 12-lead ECG reconstruction, highlight various algorithmic approaches and evaluate their predictive strengths. In addition, it investigates the implications of performing reconstruction in particular ways.</p><p><strong>Methods: </strong>This narrative review analysed 39 works on the reconstruction of 12-lead ECGs, focusing on the algorithms used for reconstruction and the results gotten from using these algorithms.</p><p><strong>Results: </strong>The works analysed featured the use of as little as one lead and as much as four leads for reconstruction of the other leads. Linear and nonlinear (including artificial intelligence) algorithms showed promising performances. Their outputs had correlations of greater than 0.90 depending on how the reconstruction models were built.</p><p><strong>Conclusion: </strong>Three leads are optimal as input predictors for minimal reconstruction errors, but there is no universal algorithm that applies to every reconstruction task. Both linear and nonlinear algorithms can achieve high correlations, and minimal root means square errors. Hence, planned steps are needed when deciding how to manipulate the data and build the models to achieve high accuracies.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1532284"},"PeriodicalIF":3.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}