Frontiers in Microbiology最新文献

{"title":"Protein elicitor PeVn1 induces resistance to <i>Botrytis cinerea</i> in strawberry and differential transcriptomic analysis.","authors":"Ziyu Zhang, Dong Wang, Yu Wang, Baozhu Dong, Jianxiu Hao, Hongyou Zhou","doi":"10.3389/fmicb.2025.1541448","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1541448","url":null,"abstract":"<p><p><i>Botrytis cinerea</i> is one of the most destructive diseases in strawberry cultivation. The protein elicitor PeVn1 is a secreted protein produced by <i>Verticillium nonalfalfae</i>, and it has been shown to enhance plant resistance against fungal infections. However, the mechanisms by which the protein elicitor acts remain poorly understood. In this study, we conducted physiological, biochemical, and transcriptomic analyses on strawberry leaves to reveal the resistance conferred by PeVn1 against <i>B. cinerea</i> infection. PeVn1 treatment significantly reduced lesion areas on <i>B. cinerea</i>-infected strawberry leaves. During the infection period, PeVn1 increased the activities of various antioxidant and defense-related enzymes, thereby enhancing the plant's oxidative capacity. Compared to inoculation with <i>B. cinerea</i> alone, malondialdehyde (MDA) and electrical conductivity levels were significantly reduced. Transcriptomic analysis identified a total of 277 differentially expressed genes (DEGs) in the leaves treated with PeVn1 compared to the control group. The three most enriched KEGG pathways were the MAPK signaling pathway, plant hormone signal transduction, and plant-pathogen interaction, all of which are associated with plant immunity. DEGs associated with plant-pathogen interaction pathways included Calmodulin-like protein 1, Calcium-dependent protein kinase, and Chitin elicitor receptor kinase 1-like protein. DEGs linked to MAPK and hormone signaling pathways included EIN3-3, ethylene-responsive transcription factor 1, MAPK9 (MKK9), and transcription factor WRKY42. These genes play critical roles in PAMP-triggered immunity (PTI) and effector-triggered immunity (ETI). They support the plant's hypersensitive response (HR), cell wall reinforcement, and other defense mechanisms. In summary, the protein elicitor PeVn1 activates the MAPK signaling pathway, increases calcium ions, and stimulates the ethylene signaling pathway in strawberry leaves, thereby enhancing plant resistance to infection. The results demonstrate that PeVn1 has significant potential to improve resistance against fungal diseases in strawberries.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1541448"},"PeriodicalIF":4.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12106544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144157770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and metabolomic insights into the biocontrol potential of <i>Bacillus velezensis</i> ZHR0 against sugarcane smut.","authors":"Kaiyuan Pan, Jun Chen, Huojian Li, Shiqiang Xu, Jihua Wang, Xiaoni Yan, Yueying Zhao, Hongtao Jiang, Abdullah Khan, Muhammad Tahir Khan, Charles A Powell, Ronghui Wen, Muqing Zhang","doi":"10.3389/fmicb.2025.1582763","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1582763","url":null,"abstract":"<p><p>Sugarcane smut, caused by <i>Sporisorium scitamineum</i>, is a major disease threatening global sugarcane production. Biological control agents (BCAs) offer environmentally sustainable alternatives to chemical fungicides, with <i>Bacillus velezensis</i> recognized for its broad-spectrum antifungal properties. In this study, <i>B. velezensis</i> ZHR0 was isolated from sugarcane leaves and evaluated for its antifungal activity through <i>in vitro</i> dual-culture assays and <i>in vivo</i> greenhouse trials. Field application of a ZHR0-based biofertilizer achieved a maximum disease control efficiency of 43.86%. Whole-genome sequencing revealed a 4.04 Mb genome with a GC content of 46.48%, encoding 4,150 genes, including multiple biosynthetic gene clusters (BGCs) associated with secondary metabolite production. <i>In vitro</i> assays showed that ZHR0 inhibited the growth of <i>S. scitamineum</i> by 53.20% and reduced disease incidence in sugarcane seedlings by 45.74%. Notably, BGCs for iturin, fengycin, surfactin, and difficidin were identified, and liquid chromatography-mass spectrometry (LC-MS) confirmed the production of iturin, supporting its role in antifungal activity. These findings demonstrate the biocontrol potential of <i>B. velezensis</i> ZHR0 against sugarcane smut and provide integrated genomic and metabolomic evidence for its application as a sustainable biocontrol agent in sugarcane cultivation.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1582763"},"PeriodicalIF":4.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12106456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144157754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modified Pulsatilla decoction ameliorates ulcerative colitis by affecting gut microbiota and metabolite profiles.","authors":"Zhixin Fu, Xiangyue Xie, Yulei Wang, Zhen Wang, Anran Wu, Shaotang Ye, Yongbo Liu","doi":"10.3389/fmicb.2025.1582559","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1582559","url":null,"abstract":"<p><p>To investigate the therapeutic effects of a specific modified Pulsatilla decoction (MPD) on ulcerative colitis (UC) in mice, 32 male Balb/c mice were randomly assigned to four groups: Control, Model, High-dose (H-dose), and Low-dose (L-dose), with eight mice per group. All groups except the Control group were administered 3% dextran sulfate sodium (DSS) in their drinking water for 7 days to induce acute UC. The H-dose group and L-dose group mice were gavaged, respectively, with different concentrations of MPD, while the Control group and Model group received the same amount of steriled water by gavage. Clinical symptoms of the mice were observed and recorded throughout the study. Subsequently, pathological sections of the colon tissues were prepared, and 16S rRNA high-throughput sequencing and metabolomics analysis were conducted on the intestinal contents. The results indicated that MPD improved the structure and morphology of colon tissue, significantly reducing inflammatory damage in DSS-treated mice. Furthermore, MPD alleviated DSS-induced intestinal injury by enhancing the abundance of beneficial intestinal probiotics, such as <i>Actinobacteriota</i> and <i>Oscillospirates</i>. Metabolomic analysis revealed significant changes in the MPD group compared to the Model group, with 53 metabolites upregulated and 22 downregulated. Key upregulated metabolites included Esculetin, Glutarate semialdehyde, and Licoricone, while downregulated metabolites included Ectoine and Trans-Piceid. KEGG enrichment analysis indicated that MPD primarily targets pathways such as linoleic acid metabolism, VEGF signaling, and glutamatergic synapse, highlighting its potential regulatory effects. In conclusion, we revealed that this MPD has the potential to alleviate DSS-induced colitis by reducing inflammation, regulating intestinal microbiota and intestinal metabolism.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1582559"},"PeriodicalIF":4.0,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene-trait matching among <i>Bifidobacterium dentium</i> strains reveals various glycan metabolism loci including a strain-specific fucosyllactose utilization cluster.","authors":"Ortensia Catalano Gonzaga, Stephen McKenna, Ian O'Neill, Paul D Cotter, Fionnuala M McAuliffe, Aidan Coffey, Douwe van Sinderen, Francesca Bottacini","doi":"10.3389/fmicb.2025.1584694","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1584694","url":null,"abstract":"<p><p>In contrast to other human-associated bifidobacteria, <i>Bifidobacterium dentium</i> is commonly classified as an opportunistic pathogen as its presence in the oral cavity has been associated with the development of dental caries. While <i>B. dentium</i> is frequently isolated from the oral cavity of children with caries, recent microbiome investigations and preliminary genomic analyses have suggested that this species is also adapted to colonize the gastrointestinal tract. Understanding the genetic and metabolic adaptations that enable this flexible colonization ability is crucial to clarify its role in human health and disease. To assess <i>B. dentium</i> genomic diversity and metabolic potential, the current study presents analysis and characterization of 10 complete genome sequences from recently isolated <i>B. dentium</i> strains obtained from human fecal samples together with 48 publicly available genome sequences. We investigated genetic loci predicted to be involved in host interaction and carbohydrate utilization in this species by means of comparative genomics, pan-genome analysis, and gene-trait matching. These analyses identified gene clusters involved in the utilization of plant-derived glycans and, for the first time, revealed <i>B. dentium</i> strains capable of utilizing human milk oligosaccharides (HMOs) through a fucosyllactose utilization cluster homologous to the one found in several infant-derived bifidobacterial species. Moreover, additional investigations of strain-specific genetic features highlighted a taxon that is evolved to colonize multiple niches and to compete with other colonizers. These findings challenge the narrow classification of <i>B. dentium</i> as an opportunist and underscore its ecological versatility.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1584694"},"PeriodicalIF":4.0,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the drug resistance mechanisms of <i>Candida tropicalis</i> and future solutions.","authors":"Huisheng Xiong, Rigetu Zhao, Shizhe Han, Zhilin Liu, Xin Zhang, Zelin Jia, Jiayu Cui, Yuhang Zhang, Xueli Wang","doi":"10.3389/fmicb.2025.1594226","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1594226","url":null,"abstract":"<p><p><i>Candida tropicalis</i> is an important member of the non-<i>Candida albicans</i> species. It is closely associated with candidemia, especially common in neutropenic and critically ill patients. Drug-resistant <i>C. tropicalis</i> isolates have been found not only in clinical patients but also in animals, fruits, and the environment. In recent years, the detection rate of azole-resistant <i>C. tropicalis</i> isolates has increased. Drug-resistant <i>C. tropicalis</i> is related to persistent, recurrent, and breakthrough infections. Therefore, understanding its drug resistance is crucial for clinical treatment. The review explores the main mechanisms of <i>C. tropicalis</i> resistance to antifungal drugs and discusses the genetic basis involved in the antifungal resistance of <i>C. tropicalis</i>. In addition, current research on natural extracts, nanomaterials, etc. used for antifungal purposes has also been reviewed. The aim of this review is that in-depth research on the drug resistance mechanisms of <i>C. tropicalis</i> resistant strains can help guide clinical medication. Meanwhile, it can also provide new ideas for opening up new pathways, searching for new targets, and screening out safe and effective \"antifungal candidates,\" with the expectation of improving the current clinical cure rates.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1594226"},"PeriodicalIF":4.0,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of inoculants on the quality of alfalfa silage.","authors":"Si-Yi Wang, Yuan-Yuan Jing, Guolin Yang, Bin Liu, Feng-Qin Gao","doi":"10.3389/fmicb.2025.1541454","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1541454","url":null,"abstract":"<p><p>To explore the effects of different silage inoculants on the silage quality of alfalfa (<i>Medicago sativa</i> L.), the experiment used Alfalfa with a moisture content of 60.00% after harvesting as the raw material. The treatments included a control group containing only distilled water (CK), Xinlaiwang I-straw silage agent (A), Xinlaiwang I-alfalfa silage agent (B), Zhuanglemei silage fermentation agent (C), Baoshiqing (D), and Kangfuqing S lactic acid bacteria inoculant (E), totaling six treatments. After 60 days of normal temperature sealing treatment, the silage fermentation and nutritional indicators of Alfalfa were measured, and the silage fermentation effect was analyzed by the membership function method. The experiment showed that when the moisture content of alfalfa was 60.00%, the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents of the silage agent treatment groups were significantly lower than those of the CK group (<i>p <</i> 0.05). The lactic acid (LA) content was significantly higher in the treatment group than in the CK group (<i>p</i> < 0.05). The addition of Xinlaiwang I-alfalfa silage agent in group B significantly increased the crude protein (CP) and LA levels in the Alfalfa silage (<i>p</i> < 0.05). It also reduced the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents. Additionally, the pH and Ammonia Nitrogen/Total Nitrogen (AN/TN) ratio were lowered. Propionic acid (PA) and butyric acid (BA) were not detected. After the membership function calculation, the average membership value of Xinlaiwang I-alfalfa silage agent (B) group was the highest with a score of 0.90, ranking first, and the silage quality was the best. In summary, through quality analysis and membership function calculation, Xinlaiwang I-alfalfa silage agent can effectively improve the silage quality of WL298HQ alfalfa.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1541454"},"PeriodicalIF":4.0,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding the C-S-R framework to incorporate microbial interactions: evidence from methane-consuming communities.","authors":"Rui Wang, Yuru Chen, Binyan Zhai, Sascha M B Krause","doi":"10.3389/fmicb.2025.1589221","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1589221","url":null,"abstract":"<p><p>Microbial interactions are critical in shaping community assembly and ecosystem functioning, yet classical ecological frameworks such as Grime's Competitor-Stress Tolerator-Ruderal (C-S-R) model primarily emphasize individual traits, overlooking interspecies dependencies. Here, we propose an expansion of the C-S-R framework to incorporate microbial interactions, using methane-consuming communities in methane-fed microcosms as a model system. We present experimental data on both natural and synthetic methane-consuming communities derived from Lake Washington sediments, demonstrating that nitrate availability regulates community dynamics and life strategies. Under nitrogen limitation, the methanotroph <i>Methylomonas</i> adopts stress tolerance via nitrogen fixation but loses its competitive advantage under nitrate-rich conditions. These shifts are linked to the emergence of <i>Methylotenera</i>, a non-methanotrophic methylotroph that relies on cross-fed carbon from methanotrophs (e.g., <i>Methylobacter</i>) and alters competitive outcomes through metabolic coupling. Our findings show that survival strategies are shaped not only by intrinsic traits but also by interaction-based traits that redistribute resources and reshape ecological niches. By integrating these dynamics, we offer a novel perspective on the C-S-R framework that captures both individual and emergent behaviors, providing new insight into microbial community resilience and improving the predictive power of ecological models under environmental change.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1589221"},"PeriodicalIF":4.0,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on soil bacterial community assembly and function under different straw returning practices in arid and semi-arid agricultural ecosystems over multiple years.","authors":"Rui-Zhi Liu, Xiao-Ya Zhao, Biao Feng, Wen-Shan Zhao, Ming-Yu Li, Xiao-Fang Yu, Shu-Ping Hu, Rui-Ping Li, Ju-Lin Gao, Qinggeer Borjigin","doi":"10.3389/fmicb.2025.1590686","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1590686","url":null,"abstract":"<p><strong>Introduction: </strong>Straw return has gained attention for its potential to improve soil quality and crop yields, particularly in semi-arid regions like the Tumu Chuan Plain Irrigation Area. Soil bacteria play a crucial role in regulating soil biological processes, and understanding how straw return affects bacterial populations can guide better agricultural management practices.</p><p><strong>Methods: </strong>We investigated the impact of continuous straw return on soil bacterial communities using 16S rRNA gene sequencing. Four treatments were applied: Farmers' shallow rotation (CK), straw incorporated with deep tillage (DPR), straw incorporated with subsoiling (SSR), and no-tillage mulching straw return (NTR). Bacterial community structure, metabolic pathways, and assembly mechanisms were analyzed using Bugbase and PICRUSt2 for phenotypic and metabolic pathway predictions.</p><p><strong>Results: </strong>The study found that straw return practices significantly altered the relative abundance and life history strategies of bacterial phyla, mainly influenced by soil organic matter (SOM) and enzyme activity. The K-strategist to r-strategist ratio was highest in CK (2.06) and lowest in SSR (1.89). DPR and NTR treatments significantly changed bacterial community structure compared to CK (<i>p</i> < 0.05), resembling SSR. Predictions showed that DPR and NTR enhanced carbohydrate and amino acid metabolism and promoted more stable bacterial networks, with homogenous selection and drift effects. Bacterial aggregation in all treatments was driven by random processes, with varying aggregation levels: CK (20%), DPR (38.6%), SSR (16.5%), and NTR (30.7%).</p><p><strong>Discussion: </strong>The study demonstrates that continuous straw return practices significantly impact soil bacterial communities. DPR and NTR notably improved microbial diversity, bacterial cooperation, and ecosystem stability. These findings provide valuable insights for sustainable agricultural practices in semi-arid regions, enhancing soil microbial ecology and soil health through strategic straw return.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1590686"},"PeriodicalIF":4.0,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root zone microbial communities of <i>Artemisia ordosica</i> Krasch. at different successional stages in Mu US Sandy Land: a metagenomic perspective with culturomics insights.","authors":"Wen Zhu, Shuo Han, Yao Cheng, Zhihong Yu, Guozhu Zhao, Xiangwei He","doi":"10.3389/fmicb.2025.1585700","DOIUrl":"10.3389/fmicb.2025.1585700","url":null,"abstract":"<p><p>Phytoremediation offers a promising strategy for addressing the global challenge of land desertification. In the Mu Us Sandy Land of China, <i>Artemisia ordosica</i> Krasch. has emerged as a key species for desertification control. Its root-associated microbial communities may enhance the plant's adaptability to sandy, nutrient-poor environments. Despite their ecological significance, comprehensive investigations of these microbial communities remain limited. In this study, microbial communities in the root zone (i.e., rhizosphere soil, non-rhizosphere soil, and root endosphere) of <i>A. ordosica</i> were analyzed via high-throughput sequencing and different isolation approaches across successional stages (moving dunes, semi-fixed dunes, and fixed dunes) in the Mu Us Sandy Land of northern China. Metagenomic analysis revealed that microbial diversity was significantly higher in the rhizosphere and non-rhizosphere soils than in the root endosphere; moving dunes exhibited lower diversity than semi-fixed and fixed dunes. Meanwhile, distinct microbial community structures across successional stages were revealed by principal coordinates analysis (PCoA), demonstrating substantial differences between the root endosphere and other zones. Environmental factors, including nitrate nitrogen (NO₃ ^--N), organic matter (OM), available potassium (AK), and total potassium (TK), significantly influenced microbial community composition. Moreover, dominant genera such as <i>Arthrobacter</i> and <i>Paraphoma</i> were identified, potentially contributing to <i>A. ordosica</i> growth. From a culturomics perspective, 93 bacterial isolates were obtained using conventional streak plate and colony pick methods, with Firmicutes (37.63%) and <i>Bacillus</i> (23.66%) identified as the dominant taxa. In parallel, 14 fungal strains were isolated, primarily belonging to <i>Penicillium</i> (35.71%) and <i>Aspergillus</i> (21.43%), both of which are well-documented for their stress tolerance in arid ecosystems. A high-throughput cultivation and identification method, tailored to recover rare and slow-growing bacteria, was employed and successfully broadened the cultured diversity to include Proteobacteria (46.43%) and representatives of the rarely cultivated Deinococcus-Thermus phylum. This study provides metagenomic with culturomics insights into the microbial communities associated with <i>A. ordosica</i>, enhancing the understanding of plant-microbe interactions in sandy land ecosystems.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1585700"},"PeriodicalIF":4.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends and characteristics of multidrug-resistant MRSA in Norway 2008-2020.","authors":"Torunn Gresdal Rønning, Hege Enger, Jan Egil Afset, Christina Gabrielsen Ås","doi":"10.3389/fmicb.2025.1564943","DOIUrl":"10.3389/fmicb.2025.1564943","url":null,"abstract":"<p><p>Infections caused by multidrug-resistant (MDR) bacteria are recognized as a critical One Health concern which poses a significant threat to public health, leading to increased morbidity and mortality across both high- and low-income countries. In this study, we investigated the epidemiology and molecular mechanisms of multidrug-resistant methicillin-resistant <i>Staphylococcus aureus</i> (MDR-MRSA) strains identified in Norway from 2008 to 2020, in order to gain a better understanding of the evolution and dissemination of multidrug resistance in <i>S. aureus</i>. A total of 452 MDR-MRSA strains isolated from 429 individuals were analyzed from a dataset of 23,412 MRSA strains. Methods included epidemiological characterization, antimicrobial susceptibility testing (AST), and genetic analysis of a selection of strains using nanopore sequencing to identify antimicrobial resistance (AMR) genes and mutations, as well as their location on plasmids, SCC<i>mec</i> and other mobile genetic elements (MGEs). The study revealed an overall increasing trend in MDR-MRSA strains, with healthcare-associated strains being more prevalent among MDR-MRSA compared to the overall MRSA population. Significant heterogeneity in <i>spa</i>-types and clonal complexes exhibiting multidrug resistance was observed, with high resistance rates against multiple antibiotic groups, particularly erythromycin, ciprofloxacin/norfloxacin, tetracycline, gentamicin, and clindamycin in addition to cefoxitin. The predominant MDR-MRSA clones included t1476/CC8, t127/CC1, t189/CC188, and t030, t037/CC239. Among these, MRSA t1476/CC8 showed an upward trend toward the conclusion of the study period, indicating the emergence of a MDR-MRSA clone. A broad range of AMR genes and mutations were detected, linked to a wide variety of MGEs, highlighting the complex mechanisms of resistance development and dissemination within the MRSA population. This study highlights the rising challenge posed by MDR-MRSA strains, and reveals the multifactorial nature of AMR in <i>S. aureus</i>, thus emphasizing the importance of continued surveillance, antibiotic stewardship and infection control measures, as well as global cooperation, in order to combat the spread of these multidrug-resistant pathogens.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1564943"},"PeriodicalIF":4.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}