Frontiers in Microbiology最新文献

{"title":"Microbiota-based interventions for autism spectrum disorder: a systematic review of efficacy and clinical potential.","authors":"Hana Taha, Ahmad Issa, Zaid Muhanna, Majdi Al-Shehab, Tuleen Wadi, Suhib Awamleh, Yousef A Ateiwi, Mohammad Abusido, Vanja Berggren","doi":"10.3389/fmicb.2025.1648118","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1648118","url":null,"abstract":"<p><strong>Purpose: </strong>Autism spectrum disorder (ASD) is increasingly linked to gut microbiota imbalances, influencing both behavioral and gastrointestinal (GI) symptoms. This systematic review assesses the efficacy of microbiota-based interventions, including probiotics, prebiotics, synbiotics, and fecal microbiota transplantation (FMT), in improving ASD-related symptoms, aiming to provide insights into their therapeutic potential and inform future clinical applications.</p><p><strong>Methods: </strong>A comprehensive systematic review was conducted following PRISMA guidelines and registered in PROSPERO (CRD42024615043). A structured literature search was performed in PubMed, Cochrane Library, and Scopus to identify peer-reviewed English-language studies. Eligible studies included randomized controlled trials (RCTs), non-randomized trials (NRTs), and retrospective studies assessing the impact of microbiota-based interventions on ASD-related behavioral and GI outcomes. Two independent reviewers conducted study selection, data extraction, and quality assessment using standardized risk-of-bias tools.</p><p><strong>Results: </strong>33 studies were included, consisting of 16 RCTs, 14 NRTs, and 3 retrospective studies. Among them, 15 assessed probiotics, 4 prebiotics, 5 synbiotics, and 9 FMT. Probiotics showed moderate behavioral improvements in ASD, with multi-strain formulations being more effective than single strains. Prebiotics and synbiotics yielded mixed results, with some studies indicating benefits in behavioral and GI symptoms. FMT demonstrated the most consistent and sustained improvements in both ASD-related behaviors and GI function. Adverse events were minimal, primarily involving transient GI symptoms.</p><p><strong>Conclusion: </strong>Microbiota-targeted interventions, particularly FMT, hold promise for managing ASD symptoms, though probiotics, prebiotics, and synbiotics present variable efficacy. Standardized protocols, larger controlled trials, and personalized microbiome-based approaches are necessary to refine these therapeutic strategies and enhance clinical applicability.</p><p><strong>Systematic review registration: </strong>https://www.crd.york.ac.uk/PROSPERO/view/CRD42024615043, identifier CRD42024615043.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1648118"},"PeriodicalIF":4.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic bacteria <i>Priestia megaterium</i> 170T-4 improves soybean salt tolerance through regulation of ion homeostasis and phytohormone signaling pathways.","authors":"Shutian Hua, Ruiyao Liu, Zhe Li, Han Zheng, Yanfen Zheng, Youqiang Wang, Cheng-Sheng Zhang, Ziyan Wang, Mingguo Jiang","doi":"10.3389/fmicb.2025.1676456","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1676456","url":null,"abstract":"<p><p>Salt stress in coastal saline-alkali soils impairs plant survival and growth. Plant growth-promoting rhizosphere bacteria (PGPR) and endophytic bacteria can enhance salinity tolerance via stable host associations. This study used culture-based and transcriptomic methods to investigate culturable endophytic bacteria in soybean and their salt-tolerance mechanisms. A total of 154 strains were isolated from the roots of 10 soybean varieties cultivated in coastal saline-alkali soil, spanning 4 phyla, 35 genera, and 76 species. <i>Microbacterium phyllosphaerae</i> and <i>Priestia megaterium</i> were identified as dominant species, from which two representative strains were selected to assess their growth-promoting effects under salt stress. Strain 170T-4 was identified as <i>P. megaterium</i> via multilocus sequence analysis and showed high salt tolerance, growing in up to 6% NaCl. Pot experiments showed that strain 170T-4 significantly improved plant height, root elongation, Na⁺/K⁺ homeostasis, proline, and chlorophyll content. Transcriptome profiling and RT-qPCR revealed that strain 170T-4 regulates K⁺ transport-related genes (<i>GORK</i> and <i>SKOR</i>), ethylene signaling related genes (<i>PTI5</i>, <i>EIN3</i>, and <i>ERF1</i>), and the allene oxide cyclase gene (<i>AOC</i>). Overall, strain 170T-4 improved soybean growth under salt stress by modulating ion transport, osmotic responses, and hormone signaling, showing strong potential as a microbial inoculant for saline-alkali soils.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1676456"},"PeriodicalIF":4.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of <i>Bifidobacterium</i> on metabolic parameters in overweight or obesity adults: a systematic review and meta-analysis.","authors":"Junmei Huang, Hao Cheng","doi":"10.3389/fmicb.2025.1633434","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1633434","url":null,"abstract":"<p><strong>Objective: </strong>This study endeavors to elucidate how <i>Bifidobacteria</i> supplementation affects metabolic parameters among overweight or obese populations.</p><p><strong>Methods: </strong>A systematic review and meta-analysis were carried out leveraging PubMed, Embase, Cochrane Library, and Web of Science. Merely randomized controlled trials (RCTs) were included.</p><p><strong>Results: </strong>21 RCTs were encompassed for our final analysis. <i>Bifidobacteria</i> supplementation was effective in weight management for overweight or obese patients. The experimental group receiving <i>Bifidobacteria</i> exhibited a marked decrease in weight (WMD: -0.607 kg; 95% CI: -0.910, -0.303, <i>I²</i> = 11.9%) and BMI (WMD: -0.214 kg/m²; 95% CI: -0.259, -0.169, <i>I²</i> = 4.1%) in contrast to the control, although the significant effect was not noted on WC. Moreover, while <i>Bifidobacteria</i> supplementation led to no marked drop in FBG or HbA1c, it improved the insulin (SMD: -0.268; 95% CI: -0.470, -0.066, <i>I²</i> = 5.4%). However, there were no evident variations in TC, TG, HDL-C, or LDL-C across groups.</p><p><strong>Conclusion: </strong>Our study findings confirmed that <i>Bifidobacteria</i> contributes to a slight reduction in weight and BMI among the overweight or obese populations, making it a potential adjunctive approach for weight management. Furthermore, it may help regulate insulin levels, though its impact on hyperglycemia and hyperlipidemia remains limited.</p><p><strong>Systematic review registration: </strong>https://www.crd.york.ac.uk/PROSPERO. Registration No. CRD42025635324.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1633434"},"PeriodicalIF":4.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12509062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota analysis reveals microbial signature for multi-autoimmune diseases based on machine learning model.","authors":"Tianfeng An, Shuya Zhang, Jinjin Li, Hui Wang, Li Chen, Yiran Shi, Jingyi Wang, Sirui Han, Ruoxi Wang, Linyuan Wang, Zijing Huan, Ruiqi Yang, Desong Hao, Yanfang Liu, Xuehua Liu, Chao Yuan","doi":"10.3389/fmicb.2025.1660775","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1660775","url":null,"abstract":"<p><strong>Introduction: </strong>Human microbiota is a major factor contributing to the immune system, offering an opportunity to develop non-invasive methods for disease diagnosis. In some research on Autoimmune Diseases (AIDs), gut microbiota variation has been observed. However, there remains a paucity of research that explores the potential of gut microbiota as a microbial signature for the classification and diagnosis of multi-AIDs.</p><p><strong>Methods: </strong>In this study, we analyzed 1,954 gut microbiota sequencing datasets from public databases collected from 1,043 patients with 10 AIDs to identify common or unique microbial signatures for AIDs through differential abundance testing and machine learning techniques. We evaluated five popular algorithms: Random Forest (RF), Support Vector Machine (SVM), K-Nearest Neighbors (KNN), Multilayer Perceptron (MLP), and eXtreme Gradient Boosting (XGBoost) models. Five-fold cross-validation and grid search were used to select the model parameters.</p><p><strong>Results: </strong>After comparing the performance of five models, the XGBoost model showed superior performance and achieved an area under the receiver operating characteristic curve (AUROC) ranging from 0.75 to 0.99 when predicting different diseases in the test set. At a specificity of 0.7 to 0.96, the sensitivity ranged from 0.66 to 1. By correlating the top 77 microbiota genera with the disease phenotypes, 126 significant associations were identified [false discovery rate (FDR) < 0.05]. We improved the detection accuracy and disease specificity for AIDs and revealed microbiota features specific to 10 different AIDs. Moreover, we found changing trends in shared microbiota features across some AID phenotypes, such as Crohn's Disease (CD) and Ulcerative Colitis (UC). At the same time, opposite changing trends were observed in the shared microbial signatures, such as Psoriasis and Myasthenia Gravis (MG). These results suggest that specific gut microbiota genera may affect the host immunity and induce different AID phenotypes.</p><p><strong>Discussion: </strong>This research holds potential for clinical application in the auxiliary diagnostic evaluation and monitoring of treatment responses. Simultaneously, it provides important clues for research on the characteristics of the intestinal immune microenvironment for different AIDs.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1660775"},"PeriodicalIF":4.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Plant mineral microbe interactions.","authors":"Muhammad Zahid Mumtaz, Tereza Cristina Luque Castellane, Izzah Shahid, Elena Tamburini, Brahim Bouizgarne","doi":"10.3389/fmicb.2025.1677458","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1677458","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1677458"},"PeriodicalIF":4.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12509987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification, biological characteristics, and screening of control agents against root rot of <i>Ardisia crenata</i> Sims in China.","authors":"Zhien Xiao, Tiantian Guo, Yuxin Liu, Yue Yuan, Shaotian Zhang, Lu Liu, Shiying He, Yanling Long, Fuqiang Yin, Ming Liu","doi":"10.3389/fmicb.2025.1618299","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1618299","url":null,"abstract":"<p><p><i>Ardisia crenata</i> Sims is included in the <i>Chinese Pharmacopoeia</i> and is a valuable traditional medicinal plant. <i>A. crenata</i> Sims root rot was first detected in 2023 in Kaizhou District, Chongqing City, China. This study was undertaken to elucidate the causal pathogen of <i>A. crenata</i> Sims root rot and the biological characteristics of the pathogen and to screen effective pesticides for its management. A pathogenic fungus was isolated and purified from typical lesions on <i>A. crenata</i> Sims, and it was identified using morphological and multi-gene combined analysis. The biological characteristics of the pathogen were investigated using the cross-inoculation method and spore counting technique, while the sensitivity of the pathogen to six chemical pesticides and six biological pesticides was evaluated using the growth rate method. The combination of morphological characteristics and polygenic association identified the pathogenic fungus as <i>Fusarium cugenangense,</i> a member of the <i>F. oxysporum</i> species complex. This is the first report of <i>F. cugenangense</i> causing root rot disease in <i>A. crenata</i> Sims. Among the six tested chemical pesticides, the 500 g/L fluazinam suspension concentrate (SC) showed the best inhibitory effect, with a half-maximal effective concentration (EC₅₀) value of 0.129 μg/mL. Among the six tested biological pesticides, 3% zhongshengmycin soluble liquid (SL) showed the best inhibitory effect, with an EC₅₀ value of 14.474 μg/mL. The conclusion is expected to lay a scientific basis for the diagnosis and control of <i>A. crenata</i> Sims root rot caused by <i>F. cugenangense</i>.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1618299"},"PeriodicalIF":4.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fitting soil extracellular enzyme activity into the complex network of abiotic and biotic soil properties often associated with soil health.","authors":"Megan G Taggart, Douglas S Baah, Sophie Allen, Zahoor Khan, Joerg Arnscheidt, Phil Jordan, Barry M G O'Hagan, Aliyu D Ibrahim, J R Rao, Nigel G Ternan","doi":"10.3389/fmicb.2025.1638267","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1638267","url":null,"abstract":"<p><p>In this mini review we examine how soil extracellular enzymes play a key role in nutrient cycling, but stress that their activity alone does not fully represent ecosystem processes. We emphasize the need for more contextual environmental data-such as pH, temperature, moisture and nutrient availability-for accurate interpretation of the significance of enzyme activity in carbon and nutrient (N, P) cycling in soil ecosystems. The importance of enzymes within the soil microbiome determines its inherent capacity to support crop growth and often reflects soil quality and soil health, which are in turn governed by multiple different soil properties. Soil enzymes (e.g., phosphatase, glucosidases, glycosaminidases) activity have been used as key soil health bio indicators for monitoring soil nutrient transformations in overgeneralized statements. Although soil enzymes constitute important attributes that are closely linked to the dynamics of soil nutrient transformation and make nutrients available to plants, we suggest a multi-factor assessment for soil health measurement. We propose that this can give a pulse reading of soil nutrient health at crucial times of soil, land use, and crop management practices but that care is required to incorporate temporal soil and land use properties for correct interpretation.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1638267"},"PeriodicalIF":4.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fecal microbiota transplantation augments 5-fluorouracil efficacy in pancreatic cancer via gut microbiota modulation.","authors":"Rui Li, Yaoyuan Hu, Yixian Liu, Xiaodong Tan","doi":"10.3389/fmicb.2025.1548027","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1548027","url":null,"abstract":"<p><strong>Background: </strong>Pancreatic cancer is a highly aggressive malignancy with limited therapeutic options due to rapid tumor progression and poor prognosis. Fecal Microbiota Transplantation (FMT) has emerged as a promising approach to modulate gut microbiota, potentially enhancing the efficacy of conventional treatments.</p><p><strong>Objectives: </strong>This study evaluates the combined effects of FMT and 5-fluorouracil (5FU) on gut microbiota composition, pancreatic tumor growth, and systemic immune responses in a murine model.</p><p><strong>Methods: </strong>One hundred female C57BL/6 mice aged 6-8 weeks were randomly divided into five groups (<i>n</i> = 20 each): Sham, Model, FMT, 5FU, and FMT + 5FU. Pancreatic tumors were induced via orthotopic implantation of Pan02 cells. FMT was administered orally (0.2 g fecal material) three times per week, starting 2 weeks before tumor implantation. 5FU was administered intraperitoneally at 25 mg/kg body weight twice weekly, beginning one-week post-tumor implantation. Gut microbiota was analyzed via 16S rRNA gene sequencing of fecal samples after 10-week cell implantation. Tumor volumes were measured, and serum cytokine levels were assessed. Short-chain fatty acids (SCFAs) in blood and feces using gas chromatography-mass spectrometry (GC-MS).</p><p><strong>Results: </strong>The FMT + 5FU group exhibited the smallest average tumor volume, significantly smaller than the Model (<i>p</i> < 0.0001) and 5FU groups (<i>p</i> = 0.005). FMT alone reduced tumor volume compared to the Model group (<i>p</i> < 0.0001). Gut microbiota analysis revealed increased <i>α</i> diversity in the FMT group compared to the Model group (<i>p</i> < 0.0001). The FMT + 5FU group showed a significant reduction in cytokine levels, including TNF-<i>α</i> (<i>p</i> = 0.0001) and IL-6 (<i>p</i> = 0.012) and increased IL-10 level (<i>p</i> < 0.001), compared to the Model group. Plasma and fecal SCFA concentrations were significantly higher in both FMT and FMT + 5FU groups relative to the Model group (<i>p</i> < 0.001). Additionally, the FMT + 5FU group had the highest survival rate (50%) after 10-week cell implantation, compared to the Model group (15%).</p><p><strong>Conclusion: </strong>FMT significantly enhances the efficacy of 5FU in reducing pancreatic tumor growth through gut microbiota modulation.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1548027"},"PeriodicalIF":4.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal dynamics and forecasting of respiratory viral infections during and after the SARS-CoV-2 pandemic (2020-2027): a multiplex PCR and ARIMA-based study.","authors":"İpek Koçer, Hadiye Demirbakan, Ahmet Aktaş","doi":"10.3389/fmicb.2025.1674529","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1674529","url":null,"abstract":"<p><strong>Introduction: </strong>Respiratory viruses are the leading cause of acute respiratory tract infections in both children and adults, with significant morbidity and healthcare burden. Before the COVID-19 pandemic, these pathogens typically exhibited predictable seasonal circulation patterns. However, the pandemic markedly disrupted this seasonality, leading to reduced viral detection during lockdowns and unusual peaks in subsequent periods.</p><p><strong>Objective: </strong>This study aimed to identify respiratory pathogens in LRTI patients using multiplex PCR and to assess changes in virus distribution during and after the COVID-19 pandemic across age groups.</p><p><strong>Methods: </strong>A total of 748 nasopharyngeal swab samples (one per patient) collected between March 2020 and November 2024 were retrospectively analyzed using the QIAstat-Dx Respiratory Panel, a multiplex PCR assay de tecting 19 respiratory viruses including SARS-CoV-2, influenza viruses, respiratory syncytial virus (RSV), and Rhinovirus/Enterovirus. Statistical analyses, including multivariate logistic regression, assessed viral positivity predictors. Additionally, the Autoregressive Integrated Moving Average (ARIMA) time-series model was used to evaluate trends and predict respiratory virus activity through 2027.</p><p><strong>Results: </strong>The cohort comprised 60.4% males and 39.6% females, with 14.4% pediatric (0-18 years) and 85.6% adult patients. Respiratory viruses were detected in 43.6% of samples, with significantly higher positivity in children (71.5%) compared to adults (40%) (<i>p</i> < 0.01). SARS-CoV-2 dominated during the pandemic (65.5% of positive cases), whereas post-pandemic viral circulation shifted toward other pathogens, notably Rhinovirus/Enterovirus (71.5%). Co-infections occurred more frequently in children (14.1%) than adults (2.7%) (<i>p</i> < 0.001). RSV re-emerged in late 2022 but was undetected in 2024, while influenza activity increased notably in early 2024. Multivariate analysis identified pediatric age as a strong independent predictor of viral positivity (OR: 3.68; 95% CI: 2.25-6.03).</p><p><strong>Discussion: </strong>Following the relaxation of public health measures, there was a marked resurgence of non- SARS-CoV-2 respiratory viruses, particularly in children, indicating a possible shift in viral epidemiology. These findings emphasize the critical need for ongoing surveillance and targeted interventions, especially in pediatric populations, to mitigate future respiratory viral disease burdens.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1674529"},"PeriodicalIF":4.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatiotemporal variations and environmental drivers of denitrifying anaerobic methane oxidizers in <i>Eriocheir sinensis</i> pond sediments.","authors":"Hongfei Zhang, Huimin Xu, Honghai Zou, Limin Fan, Xiangke Fan, Dandan Li, Longxiang Fang, Zhuping Liu, Hao Zheng, Liping Qiu, Shunlong Meng","doi":"10.3389/fmicb.2025.1679266","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1679266","url":null,"abstract":"<p><p>Denitrifying anaerobic methane oxidation (DAMO), as a coupled carbon-nitrogen cycling process, facilitates methane oxidation while enabling inorganic nitrogen removal. Crab aquaculture pond sediments, being rich in organic matter and nitrogen, may serve as hotspots for active DAMO processes. However, the presence of DAMO-functional microorganisms in such environments remains unconfirmed. In this study, we employed quantitative real-time polymerase chain reaction (qPCR) to analyze the abundance variations of DAMO functional microorganisms in crab pond sediments across different temporal (May, September, December) and vertical (0-10 cm, 10-20 cm, 20-30 cm) scales. Combined with concurrent physicochemical parameter measurements and high-throughput sequencing, the spatiotemporal distribution patterns and environmental drivers of DAMO microbiota were investigated. The results indicated that DAMO bacteria and archaea coexisted in crab pond sediments, exhibiting significant spatiotemporal heterogeneity in microbial abundance. The copy number of bacterial <i>pmoA</i> gene ranged from 2.07 × 10⁵ to 1.89 × 10⁷ copies g^-1 dry sediment, and archaeal <i>mcrA</i> gene ranged from 7.15 × 10⁵ to 1.16 × 10⁸ copies g^-1 dry sediment. The abundance of both <i>pmoA</i> and <i>mcrA</i> genes peaked in December across all sampling timepoints, with their highest enrichment in the 10-20 cm sediment layer vertically, presumably due to the synergistic effect of a stable anaerobic environment, sufficient substrate supply, and moderate environmental parameters. Temperature, pH, and nitrite concentration were identified as key environmental factors regulating DAMO microbial abundance and spatial distribution. Furthermore, both microbial community composition and diversity indices displayed pronounced spatiotemporal variability, with seasonal variations exerting stronger impacts on community structure than vertical gradients. Notably, methane-metabolizing archaea exhibited higher species diversity than methane-metabolizing bacterial communities. This study systematically elucidates the ecological distribution patterns and environmental response mechanisms of DAMO-functional microorganisms in crab pond sediments, providing a theoretical framework for methane emission mitigation strategies in aquaculture systems based on DAMO processes.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1679266"},"PeriodicalIF":4.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12509068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}