Frontiers in Microbiology最新文献

{"title":"Editorial: Microbe-environment interaction across grassland ecosystems: soil microbial diversity, plant-microbe interplay, and ecosystem functionality.","authors":"Kamran Malik, Anum Ahmad, Muhammad Usman","doi":"10.3389/fmicb.2026.1971028","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1971028","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1971028"},"PeriodicalIF":5.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149167392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effects of propyl paraben, citral, and gallic acid on the growth, patulin biosynthesis, and patulin biosynthetic gene expression of <i>Penicillium paneum</i> OM1.","authors":"Ji-Yeon Koo, Sung-Yong Hong, Ae-Son Om","doi":"10.3389/fmicb.2026.1949526","DOIUrl":"10.3389/fmicb.2026.1949526","url":null,"abstract":"<p><p><i>Penicillium paneum</i> is a phytopathogen that can cause fruit decay and produce patulin in pears. Much information is not available about inhibitory effects of food-grade natural compounds on the patulin production by <i>P. paneum</i> and its growth. In this study, we evaluated the effects of propyl paraben, citral, and gallic acid on patulin accumulation by <i>P. paneum</i> OM1 and its growth on YES (patulin supportive media). Our results showed that its patulin production was below the detection limit due to suppression by 25 μg/mL of propyl paraben or 400 μg/mL of citral and that its growth was inhibited by 14.5% and 42.8%, respectively, at the same levels of each compound. However, gallic acid did not affect the growth of the fungal strain in the range of 100-500 μg/mL, but suppressed the patulin production by 32.9%-37.8% after 7 days of incubation at 25 °C. Additionally, we assessed the expression of 5 key patulin biosynthetic genes in <i>P. paneum</i> OM1 under treatment with each compound. It exhibited that the expression of <i>patK</i>, <i>patL</i>, <i>patN</i>, <i>patG</i>, and <i>patE</i> was down-regulated in <i>P. paneum</i> OM1 except for <i>patE</i> under gallic acid supplementation. Moreover, when 0.25 μg of propyl paraben was applied onto a half of a pear, the patulin produced by <i>P. paneum</i> OM1 was not detected and the application reduced the lesion area on it by 57%. Our data demonstrated the strong inhibitory effect of the low level of propyl paraben on growth of <i>P. paneum</i> OM1 and its patulin production in pear. Our findings could provide new insights in utilization of food-grade natural compounds as antifungal and anti-mycotoxigenic agents to prevent patulin contamination of pears by <i>P. paneum</i> OM1.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1949526"},"PeriodicalIF":5.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13654974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogeological compartmentalization outweighs hydrochemistry in structuring microbial communities across contrasting subsurface habitats.","authors":"Cassandre Sara Lazar, Pierre Betti, Coralie Rolland, Jean-Sébastien Marcil","doi":"10.3389/fmicb.2026.1919949","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1919949","url":null,"abstract":"<p><p>Deep groundwater ecosystems are energy-limited microbial habitats in which hydrochemical conditions, hydrogeological structure, and habitat isolation jointly influence community assembly. However, the relative importance of environmental filtering and hydrogeological compartmentalization in structuring subsurface microbial communities remains poorly understood. We characterized hydrochemistry, bacterial communities, and archaeal signatures across two contrasting groundwater systems in Québec, Canada: a shallow permeable groundwater system at pointe-du-lac (PDL) and deep fractured formation waters (1,460-1,690 m) at saint-Flavien (SF). Hydrochemical analyses, bacterial and archaeal amplicon sequencing, occupancy-based approaches, and multivariate statistical analyses were used to evaluate patterns of community composition, persistence, and environmental association. The two groundwater systems differed markedly in hydrochemical composition, with PDL enriched in major ions and SF characterized by elevated trace element concentrations. Despite these differences, bacterial communities were structured primarily at the scale of individual wells rather than by groundwater system. Well identity explained a substantially larger fraction of community variation than hydrochemistry, indicating strong hydrogeological compartmentalization. PDL supported a larger recurrent bacterial component, stronger taxon-environment relationships, and repeated methanogen-associated archaeal signatures, whereas SF contained a smaller persistent community fraction and a greater contribution from localized taxa. Across both systems, <i>Acetobacterium</i> was the dominant bacterial genus and was largely represented by a single recurrent lineage distributed across multiple wells and hydrochemically distinct habitats. Recurrent taxa in PDL were enriched in anaerobic and sulfur-associated lineages, while SF was characterized by a smaller set of persistent groundwater-associated taxa. Methanogen-associated archaea were largely restricted to PDL and included both methylotrophic and hydrogenotrophic lineages. These results demonstrate that hydrogeological compartmentalization outweighs hydrochemistry in structuring microbial communities across contrasting subsurface habitats. The persistence of recurrent bacterial lineages, together with localized archaeal assemblages, suggests that deep groundwater ecosystems are shaped by the combined effects of hydrogeological compartmentalization, environmental filtering, and adaptation to long-term energy limitation.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1919949"},"PeriodicalIF":5.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13656802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma pre-treatment and peroxynitric acid soaking as a novel oxidative seed disinfection strategy against seed-borne fungal pathogens in maize.","authors":"Nina Recek, Jure Mravlje, Rok Zaplotnik, Jernej Ekar, Mia Jarc, Katarina Vogel Mikuš, Peter Gselman, Satoshi Ikawa, Katsuhisa Kitano, Miran Mozetič","doi":"10.3389/fmicb.2026.1927228","DOIUrl":"10.3389/fmicb.2026.1927228","url":null,"abstract":"<p><strong>Introduction: </strong>Seed-borne fungal contamination is an important challenge in maize production and may contribute to grain deterioration and mycotoxin contamination. This study evaluated peroxynitric acid (PNA), alone and in combination with nonequilibrium gaseous plasma pretreatment, as a rapid oxidative strategy for disinfecting maize seeds contaminated with <i>Aspergillus flavus</i> and <i>Fusarium fujikuroi</i>.</p><p><strong>Methods: </strong>Maize seeds were artificially inoculated with approximately 10⁶ spores of either <i>A. flavus</i> or <i>F. fujikuroi.</i> For plasma pretreatment, seeds were sequentially exposed to hydrogen (0.2 s) and oxygen (0.2 s) radio-frequency plasmas in a low-pressure reactor. Hydrogen plasma was sustained in H mode at 30 Pa and 800 W, whereas oxygen plasma was sustained in E mode at 50 Pa and 300 W. Seeds were subsequently exposed to 1 -400 mM PNA for 3 -300 s. Antifungal efficacy was assessed using direct plating assays and four-parameter logistic modeling to estimate the 50% and 90% inhibitory concentrations (IC50 and IC90) and effective exposure times (ET50 and ET90). Seed viability was evaluated by germination assays.</p><p><strong>Results: </strong>PNA exhibited rapid, concentration- and time-dependent antifungal activity against both fungal species. Strong suppression of <i>F. fujikuroi</i> infection was observed at PNA concentrations ≥100 mM, with complete suppression of detectable infection at 400 mM after 180 s. At 50 mM PNA, <i>A. flavus</i> showed substantially faster inactivation kinetics than F. fujikuroi, with estimated ET50 values of 42.3 and 95.1 s, respectively. At 100 mM PNA, plasma pretreatment shifted the fitted time -response curves toward shorter exposure times and lower infection levels, particularly for A. flavus. However, planned plasma-versus-no-plasma comparisons were not statistically significant after adjustment for overdispersion. Neither PNA nor plasma treatment significantly affected seed germination.</p><p><strong>Discussion: </strong>PNA demonstrated rapid and effective antifungal activity against seed-borne <i>A. flavus</i> and <i>F. fujikuroi</i>, with clear species-specific differences in susceptibility. Plasma pretreatment appeared to accelerate early PNA-mediated fungal suppression but did not consistently improve final disinfection efficacy at prolonged exposure times. This effect may be associated with increased seed-surface hydrophilicity following plasma exposure. The results support PNA as a promising oxidative approach for maize seed disinfection and provide further insight into species-specific responses and plasma-associated kinetic effects in seed-borne filamentous fungi.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1927228"},"PeriodicalIF":5.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13654942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of trait stacking on the maize phyllosphere microbiome: a shotgun metagenomic comparison.","authors":"Jing Ying Yan, Yuan Zhou, Liang Ni, Zhong Jie Ding, Chaoyang Lin","doi":"10.3389/fmicb.2026.1904641","DOIUrl":"10.3389/fmicb.2026.1904641","url":null,"abstract":"<p><p>Current safety assessments for stacked-trait transgenic crops underrepresent the phyllosphere, a critical interface directly exposed to transgenic products, warranting strengthened risk evaluation. Here, we employed metagenomics to characterize the phyllosphere of three maize lines under herbicide-free conditions: wild-type (Corn.CK), herbicide-resistant single-trait (transgene of <i>cp4-epsps</i>, Corn.S), and insect- and herbicide-resistant stacked-trait (transgene of <i>cry1Ab</i> and <i>cp4-epsps</i>, Corn.D). Shannon diversity showed a consistent trend toward higher values in the <i>cp4-epsps</i> single-trait line than in the wild type (not significant after correction for multiple comparisons), whereas the stacked-trait line showed an intermediate increase. Transgenic events were associated with bidirectional taxonomic remodeling, characterized by the depletion of Enterobacterales and enrichment of environmentally adapted taxa. Functionally, transgenic lines displayed pervasive metabolic depletion across KEGG pathways and CAZyme categories, alongside reduced abundance of virulence-associated gene homologs (e.g., T6SS and Enterobactin) and lower antibiotic resistance gene diversity (wild-type > stacked-trait > single-trait), driven by the concomitant loss of Enterobacterales hosts. Notably, in most of the taxonomic, functional, virulence, and resistome dimensions examined, the stacked-trait line exhibited an intermediate microbiome profile between the wild-type and single-trait lines. These findings suggest that, for this genotype combination under these controlled conditions, trait stacking was not associated with microbiome perturbation greater than that observed for the single trait, and provide a preliminary, genotype- and stage-specific reference for phyllosphere biosafety assessment, pending field validation.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1904641"},"PeriodicalIF":5.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149169969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota and health-related quality of life in paediatric inherited metabolic disorders: a narrative review and conceptual framework.","authors":"John Sieh Dumbuya, Yuanlong Li, Bo He, Guangxu Li, Yuting Liu, Youfeng Zhong, Shimei Huang, Xiuling Chen","doi":"10.3389/fmicb.2026.1928657","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1928657","url":null,"abstract":"<p><p>Inherited metabolic disorders (IMDs) comprise more than 1,400 rare genetic conditions affecting paediatric patients worldwide. Despite advances in newborn screening and dietary management, many children experience chronic complications, gastrointestinal symptoms, and reduced health-related quality of life (HRQoL). Emerging evidence-predominantly from phenylketonuria (PKU)-suggests that gut microbiota may influence metabolic homeostasis and patient-centred outcomes in affected children. Because of substantial methodological heterogeneity and the rarity of individual disorders, we elected a narrative review format to synthesise current evidence rather than a formal quantitative synthesis. Electronic searches were performed in PubMed, Embase, and Web of Science from inception to February 2026. We synthesise current evidence regarding gut microbiota composition in major paediatric IMDs, including PKU, organic acidemias, glycogen storage diseases, lysosomal storage disorders, and mitochondrial diseases. For PKU, controlled studies report reduced microbial diversity and depletion of butyrate-producing taxa associated with long-term dietary treatment. For the remaining disorders, direct paediatric microbiome data are sparse or absent, and proposed links rely on mechanistic extrapolation from non-IMD populations. We present the gut microbiota-metabolism-quality of life axis as a conceptual framework that integrates microbial metabolites, intestinal barrier function, immune signalling, and patient-reported outcomes, while emphasising that direct causal evidence linking microbiota alterations to HRQoL in paediatric IMDs is currently lacking. Microbiota-directed interventions remain preliminary. Future multicentre longitudinal studies integrating microbiome profiling, multi-omics technologies, and validated patient-reported outcomes are needed to clarify causal mechanisms and therapeutic potential. Importantly, the available evidence is heterogeneous: direct paediatric microbiome evidence is strongest for PKU, whereas evidence for other IMDs is sparse and often observational, preclinical, or extrapolated from non-IMD populations. The proposed microbiota-metabolism-HRQoL axis is therefore presented as a hypothesis-generating framework rather than an established causal pathway.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1928657"},"PeriodicalIF":5.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characteristics of a carbapenem-resistant NDM-1-producing <i>Aeromonas caviae</i> isolate from extraintestinal infection.","authors":"Zheng Bao, Zhenyu Mao, Yanye Tu","doi":"10.3389/fmicb.2026.1953006","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1953006","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;This study aims to clarify the drug resistance characteristics and genomic background of bile-derived NDM-1-producing &lt;i&gt;Aeromonas caviae&lt;/i&gt; (&lt;i&gt;A. caviae&lt;/i&gt;) isolate from extraintestinal infection, providing a theoretical basis for the prevention and control of clinical infections.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A bile-derived LHL25108073-&lt;i&gt;A. caviae&lt;/i&gt; isolate (NCBI accession number: JBSUSV000000000) was chosen as the study focus. Through flight mass spectrometry, VITEK-2 drug susceptibility phenotype validation, and whole-genome sequencing, multi-point sequence typing (MLST), VFDB database, CARD database, PlasmidFinder 2.1 software, whole-genome alignment, plasmid synteny analysis, and plasmid conjugation assay were applied for an integrative analysis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;This isolate was identified to be ST2281, a sequence type rarely reported across clinical, environmental, and agricultural sources, with a genome size of 4,885,524 bp and a GC content of 61.27%, carrying one chromosome (4,687,937 bp) and one drug-resistant plasmid (197,587 bp). A total of 58 high-confidence virulence genes were detected, covering common flagella, polar flagella, lateral flagella, type IV pili, Msh pili, type VI/II secretion system (T6SS/T2SS), heme-uptake systems, Endotoxins, and hemolysin, forming multiple pathogenic mechanisms. Among 14 drug resistance genes, the carbapenem-resistant NDM-1 gene was highly consistent with the genotype, only sensitive to a few drugs such as amikacin and aztreonam, based on the results of drug susceptibility tests. The drug-resistant plasmid could not autonomously conjugate and transfer due to the loss of the oriT site and was untypable by conventional plasmid typing. This plasmid shared 99-100% homology in the resistance core regions with two NDM-1-carrying &lt;i&gt;A. caviae&lt;/i&gt; plasmids, pK433-NDM (OK287926) from sputum in Taizhou, Zhejiang, and pFAHZZU2447_NDM (CP100393) from bile in Zhengzhou, with identical &lt;i&gt;bla&lt;/i&gt; &lt;sub&gt;NDM-1&lt;/sub&gt; sequences. Genetic background analysis based on the genomes of 157 &lt;i&gt;A. caviae&lt;/i&gt; isolates from China and neighboring regions identified that ST313 was the dominant sequence type (ST), which accounted for the largest proportion among in Chinese isolates.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;This study characterizes the molecular profiles of an extraintestinal, bile-derived NDM-1-producing &lt;i&gt;A. caviae&lt;/i&gt; isolate belonging to ST2281, validates its abundant reservoir of virulence genes and comprehensive multi-drug resistance mechanisms, and further reveals the potential risk of NDM-1 dissemination via alternative horizontal gene transfer pathways, such as transformation and transduction. Genomic evolutionary analysis reveals that although the ST2281 isolate identified in this study shares certain phylogenetic relatedness with the prevalent ST313 lineage in China at the housekeeping gene level, whole-genome SNP-based phylogenetic analysis confirms a s","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1953006"},"PeriodicalIF":5.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13656038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A metagenomic snapshot of metabolic potentials in wintertime eutrophic sediments of Lake Taihu inferred from metagenome-assembled genomes.","authors":"Qi Wei, Yajie Li, Aidong Ruan","doi":"10.3389/fmicb.2026.1929411","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1929411","url":null,"abstract":"<p><strong>Introduction: </strong>Understanding the metabolic potential of microbial communities in eutrophic lake sediments at the genome-resolved level is essential for predicting nutrient cycling and stress responses in contaminated freshwater ecosystems.</p><p><strong>Methods: </strong>In this study, we reconstructed 206 dereplicated metagenome-assembled genomes (MAGs) from sediment cores collected across four regions of Lake Taihu.</p><p><strong>Results: </strong>These dereplicated MAGs spanned 34 classified phyla and 72 classified genera, with 97.6% unclassified at the species level, indicating substantial genomic novelty. The cyanobacterial MAGs harbored diverse secondary metabolite biosynthetic gene clusters, suggesting a genomic basis for potential adaptive traits. Across all dereplicated MAGs, the highest biosynthetic potential for secondary metabolites was observed in Chloroflexota and Pseudomonadota. Putative antibiotic resistance genes were predominantly represented by glycopeptide resistance determinants (vanT/vanW) and resistance-nodulation-division (RND) efflux pumps, which together accounted for 86.1% of the detected resistome. Additionally, 490 predicted horizontal gene transfer (HGT) events were identified, with transferred genes being most prevalent in energy metabolism (12.4%), translation (10.8%), and transcriptional regulation (7.2%).</p><p><strong>Discussion: </strong>This genome-resolved survey provides a foundation for understanding the metabolic and genomic potential of sediment microbiomes in eutrophic freshwater systems.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1929411"},"PeriodicalIF":5.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13656212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Moldogenicity of dominant culturable molds in cigar tobacco leaves and GC-IMS-based analysis of volatile organic compounds.","authors":"Kuo Huang, Ge Zhang, Mengmeng Liu, Shaoxin Yang, Chen Chen, Beibei Zhu, Min Wei, Yong Pan, Xiaoyan Gao, Dong Li, Delong Xu, Fang Zheng, Fang Xue, Changwen Ye","doi":"10.3389/fmicb.2026.2004227","DOIUrl":"https://doi.org/10.3389/fmicb.2026.2004227","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fmicb.2026.1843382.].</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"2004227"},"PeriodicalIF":5.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13656801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of lyophilized <i>Lactiplantibacillus plantarum</i> DC2 probiotic powder on gastrointestinal morphology and microbiota in mice.","authors":"Juzuo Zhang, Jun Jiang, Mingyu Wang, Demiao Zeng, Junfei Xu","doi":"10.3389/fmicb.2026.1858099","DOIUrl":"10.3389/fmicb.2026.1858099","url":null,"abstract":"<p><p>Probiotics have potential benefits in modulating gastrointestinal function. However, their efficacy and application depend largely on specific bacterial strains and environmental contexts. In our previous study, a strain of <i>Lactiplantibacillus plantarum</i> (<i>L. plantarum</i>) DC2 exhibited superior functionality in pork fermentation processes and was granted a Chinese patent (ZL202110743486.8). However, its storage and application characteristics remain unclear. To evaluate its direct application potential for gastrointestinal regulation, in this study, the formula and process of lyophilized <i>L. plantarum</i> DC2 powder were optimized via single-factor design (SFD) and response surface methodology (RSM). The optimized protectant consisted of fat-free milk powder, trehalose, glycerine, and L-sodium glutamate. The survival rate and storage stability of lyophilized bacterial powder were maintained at optimal level under optimized conditions. Intragastric administration experiments revealed that the abundance of <i>Lactobacillus</i> in both the stomach and jejunum significantly increased, accompanied by shifts in intestinal morphological features and modulation of the intestinal microbial composition and abundance. In conclusion, a reliable lyophilization protocol was established for optimal survival rate and storage stability of lyophilized <i>L. plantarum</i> DC2 powder, which was capable of modulating intestinal morphology and gut microbial profiles in mice. This study lays a foundation for its further development as a probiotic candidate.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1858099"},"PeriodicalIF":5.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}