Frontiers in Microbiology最新文献

{"title":"Culture-based characterization of gut microbiota in inflammatory bowel disease.","authors":"Hyunjoon Park, Soyoung Yeo, Taekyu Lee, Yumin Han, Chang Beom Ryu, Chul Sung Huh","doi":"10.3389/fmicb.2025.1538620","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1538620","url":null,"abstract":"<p><p>Inflammatory bowel disease (IBD) is characterized by disruptions in the gut microbiome. While most studies on gut dysbiosis in IBD rely on sequencing-based methods, we employed a streamlined culturomics approach to obtain a more comprehensive understanding of gut microbiota imbalance in patients with IBD that may not be captured by sequencing alone. A total of 367 bacteria were identified at the species level, including 211 species from ulcerative colitis patients, 164 species from Crohn's disease (CD) patients, and 263 species from healthy individuals. Consistent with our 16S rRNA gene amplicon sequencing results, a significant decrease in microbial diversity and a severe imbalance, especially in CD patients, were also observed in the culture-based analysis. Our culturomics approach provided additional insights, highlighting dysbiosis in unique anaerobic and Gram-negative species in CD patients. Moreover, species-level findings for <i>Bifidobacterium</i> and <i>Enterobacterales</i> emphasized specific species expansions in IBD patients. Notably, <i>Mediterraneibacter gnavus</i>, <i>Thomasclavelia ramosa</i>, <i>Parabacteroides merdae</i>, and <i>Collinsella aerofaciens</i> are of particular clinical interest due to their correlation with inflammatory biomarkers. This comprehensive analysis underscores the value of integrating a culture-based approach with a genome-based approach to provide complementary insights and therapeutic targets in IBD.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1538620"},"PeriodicalIF":4.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI applications in HIV research: advances and future directions.","authors":"Ruyi Jin, Li Zhang","doi":"10.3389/fmicb.2025.1541942","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1541942","url":null,"abstract":"<p><p>With the increasing application of artificial intelligence (AI) in medical research, studies on the human immunodeficiency virus type 1(HIV-1) and acquired immunodeficiency syndrome (AIDS) have become more in-depth. Integrating AI with technologies like single-cell sequencing enables precise biomarker identification and improved therapeutic targeting. This review aims to explore the advancements in AI technologies and their applications across various facets of HIV research, including viral mechanisms, diagnostic innovations, therapeutic strategies, and prevention efforts. Despite challenges like data limitations and model interpretability, AI holds significant potential in advancing HIV-1 management and contributing to global health goals.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1541942"},"PeriodicalIF":4.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-enveloped virus inactivation potency of sodium dodecyl sulfate with citric and glutamic acids.","authors":"Yong Wah Tan, Chwee Fern Bok, Brenda Jun Fang Lim, Li Ying Kong, Kian Sim Goo, Yoshiki Ishida, Jiquan Liu, Chun Song Chua, Justin Jang Hann Chu","doi":"10.3389/fmicb.2025.1535404","DOIUrl":"10.3389/fmicb.2025.1535404","url":null,"abstract":"<p><p>Disinfection is one of the most important methods by which transmission of infectious diseases can be blocked, and efficacies differ depending on how they are used and the target organism. Small non-enveloped viruses are considerably less sensitive to disinfectants than enveloped viruses and vegetative bacteria or fungi and generally require strong protein-disrupting chemicals for effective inactivation, limiting their application in personal care products due to associated side effects. Sodium dodecyl sulfate (SDS) is a common anionic surfactant and relatively safe ingredient used in many personal care and hygiene products possessing protein-denaturing properties and has been reported to have antimicrobial efficacy against enveloped viruses and bacteria. With the aim of identifying milder disinfectants with broad-spectrum activity, including efficacy against non-enveloped viruses that are more difficult to inactivate, this study focused on evaluating the combinatorial efficacy of sodium dodecyl sulfate with organic acid (i.e., citric acid) and amino acid (i.e., glutamic acid) on feline calicivirus. Using an <i>in vitro</i> quantitative suspension test and electron microscopy, we have demonstrated the virucidal efficacy of SDS combinations with citric or glutamic acids on FCV. In addition, the spectrum of virucidal efficacy may potentially be extended to some human enteroviruses, and further research into their variable sensitivity to virus inactivation would be useful in developing these combinations into consumer products that target non-enveloped viruses.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1535404"},"PeriodicalIF":4.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11880254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphate-solubilizing microorganisms for soil health and ecosystem sustainability: a forty-year scientometric analysis (1984-2024).","authors":"Yiming Lei, Yuhan Kuai, Mingyu Guo, Huan Zhang, Yuan Yuan, Hualong Hong","doi":"10.3389/fmicb.2025.1546852","DOIUrl":"10.3389/fmicb.2025.1546852","url":null,"abstract":"<p><p>Phosphate-solubilizing microorganisms (PSM) play a crucial role in promoting crop growth by enhancing phosphorus supply and reducing phosphorus loss in soil. However, a comprehensive bibliometric overview of the research landscape on PSM in agricultural applications has been lacking. This study conducts a bibliometric analysis to explore global research trends, key contributors, and collaborative networks in the application of PSM in ecological restoration, providing valuable insights for future research. A total of 1,662 documents from the Web of Science Core Collection, spanning from 1984 to 2024, were extracted and analyzed using Bibliometrix and CiteSpace software. The findings reveal a period of rapid growth in this field since 2018. Initially, research focused on microbial soil nutrients, such as phosphate rock and <i>Azospirillum brasilense</i>. Current research hotspots have shifted towards topics like drought and salt stress, as well as productivity, reflecting an increasing emphasis on mitigating the impacts of global warming and environmental changes. China and India lead in research output, contributing 36.67% of the total articles. The Indian Council of Agricultural Research published the highest number of articles. Future research on PSM should emphasize their role in enhancing nutrient uptake, improving soil health, and mitigating environmental stresses, supporting sustainable agriculture and ecological restoration. This bibliometric analysis of 1,162 articles by 7,454 authors from 101 countries highlights critical advances at the intersection of soil microbiology, sustainable land management, and climate change adaptation. These findings provide a foundation for addressing global challenges like soil degradation, nutrient cycling, and food security, aligning with the Sustainable Development Goals.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1546852"},"PeriodicalIF":4.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pasteurized <i>Akkermansia muciniphila</i> Timepie001 ameliorates DSS-induced ulcerative colitis in mice by alleviating intestinal injury and modulating gut microbiota.","authors":"Huan Han, Hui Xiong, Zengli Liu, Xunzhi Liu, Hailin Wang, Jiaxiang Kou, Dewei Yi, Ying Shi, Hao Wu, Jianjun Qiao","doi":"10.3389/fmicb.2025.1542522","DOIUrl":"10.3389/fmicb.2025.1542522","url":null,"abstract":"<p><strong>Introduction: </strong><i>Akkermansia muciniphila</i> (<i>A. muciniphila</i>), known as a next-generation probiotic, has been widely recognized for its beneficial effects in various metabolic diseases. While there is not much research whether live or pasteurized <i>A. muciniphila</i> has different effects on intestinal health.</p><p><strong>Methods: </strong>In the present study, a strain of <i>A. muciniphila</i> was isolated from healthy individuals, with the live and pasteurized <i>A. muciniphila</i> named Timepie001 and Timepie001+, respectively. They were administered to dextran sulfate sodium-induced ulcerative colitis mice to investigate their influences on the host intestinal health.</p><p><strong>Results and conclusion: </strong>The results showed that prophylactic supplementation with live and pasteurized <i>A. muciniphila</i> alleviates ulcerative colitis symptoms by retarding weight loss, preserving intestinal tissue structure, modulating inflammatory cytokines (TNF-α, IL-1β), and enhance the colonic mucosal barrier by upregulating the expression of tight junction protein Claudin-1. Interestingly, pasteurized <i>A. muciniphila</i> has a better effect compared with live <i>A. muciniphila</i>. Moreover, pasteurized <i>A. muciniphila</i> can regulates the gut microbiome to maintain intestinal homeostasis. This provides theoretical support for the widespread application of postbiotics in the food industry.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1542522"},"PeriodicalIF":4.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11880272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal dynamics of bacterial communities in mangrove sediments of Shupaisha island, Zhejiang Province, China.","authors":"Maoqiu He, Shoudian Jiang, Xiangfu Li, Liqin Yao, Bonian Shui","doi":"10.3389/fmicb.2025.1526730","DOIUrl":"10.3389/fmicb.2025.1526730","url":null,"abstract":"<p><p>Mangrove forests, found primarily in tropical and subtropical coastal regions, support diverse microbial communities that are crucial for nutrient cycling and energy flow and then sustain mangrove ecosystem integrity. This study investigated the seasonal dynamics of bacterial communities in mangrove sediments of Shupaisha island (Zhejiang Province, China) through amplifying and high-throughput sequencing bacterial 16S rRNA gene fragments. Proteobacteria (23.59-44.40%), Actinobacteria (4.92-19.01%), and Bacteroidetes (4.31-22.79%) dominated the bacterial phyla in Shupaisha mangrove sediments with the highest diversity indices in winter. Actinobacteria were more abundant during winter (13.27%) and spring (14.36%), while Bacteroidetes abundance was highest in summer, significantly correlating with temperature. Significant differences in bacterial community composition were observed between winter and summer, while spring and autumn exhibited similar distribution, indicating a transitional pattern in bacterial community dynamics, with temperature and sand content being the most influential factors. This study enhances our understanding of the seasonal characteristics of bacterial communities in the mangrove ecosystems, potentially providing valuable insights into monitoring and assessing the health and stability of mangrove ecosystems in Zhejiang Province.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1526730"},"PeriodicalIF":4.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11880258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The distribution of aerobic bacteria in Chinese cropland is linked to the soil texture.","authors":"Xiao Jiamin, Wu Haonan, Xu Chao, Hu Yajun, Xu Zhiwen, Zhang Dongxu, Li Abo, Wei Xiaomeng, Ge Tida, Wei Gehong","doi":"10.3389/fmicb.2025.1541460","DOIUrl":"10.3389/fmicb.2025.1541460","url":null,"abstract":"<p><p>Aerobic bacteria extensively drive the carbon cycle in soil owing to their vigorous respiration; however, their geographical distribution and mechanisms remain poorly understood. The citric acid synthetase-encoding gene (<i>gltA</i>), which encodes the key enzyme in the tricarboxylic acid cycle of aerobic respiration, was used as a marker gene to investigate the geographical distribution of aerobic bacteria in Chinese agricultural fields. The abundance and diversity of <i>gltA</i>-harboring bacteria changed unimodally as the latitude increased, with peak values at middle latitudes, where the dominant species showed the lowest relative abundance. Despite the different water management practices, our data found little difference in the abundance, diversity, or relative abundance of the dominant species of <i>gltA</i>-harboring bacteria between paddy and upland soils on a large scale, which was significantly affected by the soil type (black, fluvo-aquic, and red), which can be defined by the soil texture. Linear regression and random forest model analyses indicated that soil texture strongly regulated the community of <i>gltA</i>-harboring bacteria, particularly the abundance of this functional guild. Generally, less abundant and diverse <i>gltA</i>-harboring bacteria were observed in soils with higher clay content. We identified biomarkers in the different soil types using linear discriminant analysis effect size analysis. The results suggest a significant correlation between soil texture and most of these biomarkers. Additionally, the biomarkers in black soil were mainly <i>r</i>-strategists, which include <i>Proteobacteria, Actinobacteria</i>, and <i>Bacteroidetes</i>, were positively correlated with soil organic carbon content. In contrast, the biomarkers in fluvo-aquic soil were generally <i>K</i>-strategists, such as <i>Acidobacteria, Ktedonobacteraceae, Planctobacteriaceae</i>, and <i>Frankia</i> were negatively correlated with soil organic carbon content. These different biomarkers likely play distinct roles in soil carbon sequestration. This study provides foundational insights into the role of aerobic bacteria in soil and enhances our understanding of microbial contributions to the biogeochemical cycle of carbon.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1541460"},"PeriodicalIF":4.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11880006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolated from Populus euphratica rhizosphere soil, and mining their metabolites.","authors":"Jia-Xin Zhang, Yi-Huang Chen, Xinrong Luo, Li-Li Zhang, Xiao-Xia Luo","doi":"10.3389/fmicb.2025.1530786","DOIUrl":"10.3389/fmicb.2025.1530786","url":null,"abstract":"<p><strong>Introduction: </strong>The microbial community in desert ecosystems is a vital and highly active component. <i>Streptomyces</i>, one of the dominant genera within this community, exhibits significant capabilities in metabolic degradation and the synthesis of secondary metabolites.</p><p><strong>Methods: </strong>To investigate the metabolic potential of <i>Streptomyces desertis</i>, two strains of <i>Streptomyces</i> were isolated from rhizosphere soil samples of <i>Populus euphratica</i> in the Taklimakan Desert during the initial phase of this study, TRM 70351^T and TRM 70361^T.</p><p><strong>Results: </strong>The identification of these strains as belonging to the genus <i>Streptomyces</i> was confirmed through 16S rRNA sequencing. All calculated Average Nucleotide Identity (ANI) values were below the 95% cut-off recommended for distinguishing distinct species, and the estimated digital DNA-DNA hybridization (dDDH) values were all below the suggested threshold of 70% for species delineation. Results from phylogenetic, genomic, phenotypic, and chemotaxonomic analyses confirmed that TRM 70351^T and TRM 70361^T represent two new species within the genus <i>Streptomyces</i>, designated as <i>Streptomyces solitudini</i>s sp. nov. and <i>Streptomyces rubellus</i> sp. nov. The type strain for <i>Streptomyces solitudinis</i> sp. nov. is TRM 70351^T ( = CCTCC AA 2020049^T = LMG 32404^T), while the type strain for <i>Streptomyces rubellus</i> sp. nov. is TRM 70361^T ( = CCTCC AA 2020043^T = JCM 35793^T). Notably, Among the identified gene clusters of TRM 70351^T, cluster 12.1 was predicted to be the biosynthetic gene cluster responsible for producing the aminoglycoside compound streptomycin, exhibiting a similarity of 55%. In this study, HSQC-TOCSY was employed to detect the presence of aminoglycosides in fermentation medium No. 1, while LC-MS/MS was utilized to analyze the molecular fragments of neomycin and streptomycin in the alkaline aqueous phase sample of the fermentation product. The mixture was eluted using methanol and ammonia water in a 3:1 ratio, leading to the further separation of the compounds daidzein and Tridec-1-ene.</p><p><strong>Discussion: </strong>This study has enhanced the species resources of <i>Streptomyces deserticum</i> and the diversity of aminoglycoside compound-producing bacteria. TRM 70351^T exhibited unique metabolic potential, indicating that further studies could be conducted in the future.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1530786"},"PeriodicalIF":4.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota mediates the pro-pyroptosis effect of xierezhuyubuxu decoction in hepatocellular carcinoma.","authors":"Zhili Zeng, Mingxuan Feng, Fan He, Enxin Zhang, Xiushen Li, Zebiao Cao","doi":"10.3389/fmicb.2024.1481111","DOIUrl":"10.3389/fmicb.2024.1481111","url":null,"abstract":"<p><strong>Introduction: </strong>Xierezhuyubuxu decoction (XRZYBXD) is prepared by adding and reducing the Dahuang Zhechong Pill, which is a traditional Chinese medicinal formula in \"The Synopsis of Prescriptions of the Golden Chamber\". XRZYBXD has previously been reported to have good efficacy in treating Hepatocellular carcinoma (HCC) in clinical and basic research. However, its underlying mechanism in treating HCC has not been fully elucidated. The aim of the study is to investigate the pro-pyroptosis effect of XRZYBXD in HCC and the role of gut microbiota in this process.</p><p><strong>Methods: </strong>Firstly, we executed comprehensive analyses of XRZYBXD on pyroptosis, intestinal flora, microbial metabolites and intestinal barrier function using TUNEL, IHC, ELISA, WB, Q-PCR, 16S rRNA sequencing, and untargeted metabolomics in a H22 tumor-bearing mice model. Further, through rescue experiment of antibiotics-induced microbiota depletion and fecal microbial transplantation (FMT) experiment, the mechanism of XRZYBXD promoting pyroptosis of HCC by improving intestinal flora was verified.</p><p><strong>Results: </strong>We found that XRZYBXD medium and high dose significantly inhibited the growth of tumor and induced pyroptosis of hepatoma cells. They also modified intestinal ecological disorders by expansion of the abundance of beneficial bacteria (such as <i>Akkermansia muciniphila</i> and <i>Parabacteroides distasonis</i>) and reduction of the abundance of harmful bacteria (such as <i>Barnesiella intestinihominis</i>). Accordingly, microbiota metabolites and intestinal barrier function were also significantly improved by XRZYBXD.</p><p><strong>Discussion: </strong>Further, elimination of gut microbiota by antibiotics weakened the efficacy of XRZYBXD, and FMT with feces from the XRZYBXD high dose group achieved similar therapeutic efficacy as XRZYBXD. In brief, XRZYBXD promote pyroptosis of hepatoma cells via adjusting intestinal dysbiosis.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1481111"},"PeriodicalIF":4.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11880294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric analysis of global research on the clinical applications of aminoglycoside antibiotics: improving efficacy and decreasing risk.","authors":"Tengxiang Zhao, Nan Chen, Mingyue Zhang, Likai Lin, Bin Lin, Yuan Fang, Zhihui Hua, Chenyu Liang","doi":"10.3389/fmicb.2025.1532231","DOIUrl":"10.3389/fmicb.2025.1532231","url":null,"abstract":"<p><strong>Background: </strong>Infections by drug-resistant bacteria are a significant threat to human health worldwide although many drug-resistant bacteria are sensitive to aminoglycosides (AGs), an older class of antibiotics. AGs have played a significant role in clinical practice in recent years.</p><p><strong>Methods: </strong>Publications from 1 January 2013 to 31 December 2023 that described clinical research of AGs were identified by searching the Web of Science Core Collection Database. Visual presentations of different bibliometric networks were prepared using VOSviewer and CiteSpace.</p><p><strong>Results: </strong>There were 915 eligible publications and the annual number of publications increased over time. The United States had the most publications and was at the core of the cooperative network. Italy and Belgium had the highest quality publications, and many of the institutions with high yield and high research quality were in Australia. JA Roberts (University of Queensland, Australia) was the most productive author and was the author of many high-quality studies in cooperation with various other researchers. The majority of publications were in journals that focused on antibacterials, chemotherapy, and pharmacokinetics. Analysis of the most highly cited publications, references, and keywords, indicated that this research mainly focused on infections by drug-resistant bacteria, drug administration in vulnerable populations, safety, pharmacokinetics, combination therapy, and new methods of administration.</p><p><strong>Conclusion: </strong>AGs have an increasingly important role in the treatment of infections by multidrug-resistant bacteria. Therapeutic drug monitoring should be performed in vulnerable populations, such as the elderly, children, and infants, to improve efficacy and reduce toxicity. Avoiding prolonged dosing cycles and refraining from using AGs in patients with the m.1555 A > G gene variant can significantly mitigate the risk of ototoxicity. Future studies should examine the pharmacokinetic and pharmacodynamic targets of AGs and assess the efficacy and safety of administration by inhalation to improve efficacy and decrease risk.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1532231"},"PeriodicalIF":4.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11880276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}