Frontiers in Microbiology最新文献

{"title":"Characterization of acidic lysine acylations in mycobacteria.","authors":"Tong Ye, Danfeng Wang, Yewen Sun, Shuyu Xie, Tianqi Liu, Nana Tian, Minjia Tan, Jun-Yu Xu","doi":"10.3389/fmicb.2024.1503184","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1503184","url":null,"abstract":"<p><strong>Introduction: </strong>Protein acetylation is an extensively investigated post-translational modification (PTM). In addition to lysine acetylation, three new types of lysine acylations characterized by the presence of an acidic carboxylic group have been recently identified and validated. These included lysine malonylation (Kmal), lysine succinylation (Ksucc) and lysine glutarylation (Kglu). Pathogens belonging to the genus Mycobacterium elicit severe diseases in mammalian hosts through the modulation of energy metabolism pathways. Throughout this process, malonyl-CoA, succinyl-CoA and glutaryl-CoA are important intermediates in metabolic pathways, including the tricarboxylic acid (TCA) cycle, amino acid and lipid metabolism. These short-chain acyl-CoAs serve as substrates for corresponding acidic lysine acylation reactions. However, the landscape of these acyl-CoAs dependent acidic lysine acylomes remains unclear.</p><p><strong>Methods: </strong>We used the high-affinity antibody enrichment combined with high-resolution LC-MS/MS analysis to systematically investigate the global proteomic characteristics of the three acidic lysine acylations in <i>Mycobacterium smegmatis</i>. Subsequently, we employed in vitro enzymatic assays to validate the functional impact of acylated substrates, adenylate kinase and proteasome-associated ATPase. Furthermore, we investigated the effects of overexpressing these two substrates on the in vitro growth of <i>Mycobacterium smegmatis</i>, its invasion of THP-1 cells, and the influence on inflammatory cytokines.</p><p><strong>Results: </strong>We systematically investigated the global substrate characterization of 1,703 lysine malonylated sites, 5,320 lysine succinylated sites and 269 lysine glutarylated sites in the non-pathogenic model strain <i>Mycobacterium smegmatis</i>. Bioinformatics analysis demonstrated a correlation between these acidic lysine acylations and the functional roles of ribosomes, in addition to their roles in various metabolic pathways. Furthermore, we investigated the impact of lysine acylations on the functional activity of adenylate kinase and proteasome-associated ATPase, as well as their roles in mycobacterial infection process.</p><p><strong>Discussion: </strong>Collectively, our study provided an important resource on substrate characterization and functional regulation of acidic lysine acylations in <i>Mycobacterium smegmatis</i>, giving valuable insights into their interrelation with the biology of infectious process.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1503184"},"PeriodicalIF":4.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11667787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into the microbial diversity and community in the sacrificial pits of Sanxingdui site (Sichuan, China).","authors":"Ruru Chen, Zhenbin Xie, Qing Xiao, Chong Wang, Rui Wen","doi":"10.3389/fmicb.2024.1489025","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1489025","url":null,"abstract":"<p><strong>Introduction: </strong>The Sanxingdui site (Sichuan, China) is the typical representative of the ancient Shu culture, which lasts from the late Neolithic to early Western Zhou. The sacrificial pits are located in the core region of Sanxingdui site, and numerous artifacts are unearthed including ivory, seashells, bronzes, pottery, jade, stone, gold, bone, and horn products. The function of the pits and buried artifacts has always been the focus, but the microbiome around artifacts attracts less attention. Recently, the microbiome in buried ivory soil has just been identified; however, the microbiome around other artifacts has never been studied. In term of the unique perspective for interpretation the archaeological issues, the study was carried out for: (1) the microbial diversity and community of soil in the lower layer of artifacts in sacrificial pits, (2) the potential biodeterioration behavior of organic and inorganic relics, and (3) the impact of sacrificial and burial activities in different sacrificial pits on microbiome.</p><p><strong>Methods: </strong>There were 45 soil samples around different artifacts in three sacrificial pits and 12 raw soil samples inside or outside the sacrificial pit sampling from Sanxingdui site. The microbial genomes were then identified and analyzed using the next-generation high-throughput sequencing.</p><p><strong>Results: </strong>The represented bacterial phyla were Proteobacteria, Actinobacteriota, GAL15, Chloroflexi, Acidobacteriota, Methylomirabilota, Thermoplasmatota, Crenarchaeota, Gemmatimonadota, and Firmicutes, and the represented fungal phyla were Ascomycota, Mortierellomycota, and Basidiomycota. Further microbial functional analysis found that the bacterial genera <i>Sphingopyxis</i>, <i>Limnobacter</i>, and <i>Streptomyces</i> and the fungal genera <i>Cladosporium</i>, <i>Acremonium</i>, and <i>Mortierella</i> were concerned with the degradation of organic matter, while the genera <i>Pseudomonas, Arthrobacter</i>, <i>Variovorax</i>, <i>Aspergillus</i>, and <i>Penicillium</i> might be related to the biocorrosion of bronzes. In addition, the microbial composition and principal co-ordinate analysis (PCoA) demonstrated the significant differences in microbial composition and structure between the raw soil samples and the soil samples around the artifacts and also between the soil samples in different sacrificial pits.</p><p><strong>Discussion: </strong>It is important to understand the biodeterioration of the buried artifacts and the sacrificial activities in Sanxingdui site according to the results of microbial diversity and community. The combination of microbiology and archaeology will shed light on the archaeological issues related to the ancient human activities and behaviors.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1489025"},"PeriodicalIF":4.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11666563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Emerging trends and advances in the socioeconomic applications of beneficial microbes.","authors":"Laurent Dufossé, Pragya Tiwari","doi":"10.3389/fmicb.2024.1523569","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1523569","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1523569"},"PeriodicalIF":4.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11666504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: New insights in microbial stress tolerance mechanisms.","authors":"Zeynep Petek Çakar, Hector Alex Saka, Jose Echenique","doi":"10.3389/fmicb.2024.1513485","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1513485","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1513485"},"PeriodicalIF":4.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11666507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and evaluation of <i>Cyberlindnera fabianii</i> strains to improve cigar tobacco leaves fermentation effect.","authors":"Sida Guo, Yasen Li, Zhen Yang, Qianying Zhang, Pinhe Li, Zhongrong Jiang, Jiabao Zhang, Yu Cao, Zhengcheng Zhang, Dongliang Li","doi":"10.3389/fmicb.2024.1492042","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1492042","url":null,"abstract":"<p><strong>Introduction: </strong>Fermentation is a crucial process in cigar manufacturing. Exogenous addition of functional microorganisms for fortified fermentation can further improve cigar tobacco leaves fermentation quality.</p><p><strong>Methods: </strong>In this study, five <i>Cyberlindnera fabianii</i> strains were isolated from cigar tobacco leaves. Their fermentation effects were assessed by analyzing flavor components and major chemical compositions.</p><p><strong>Results: </strong>Among these five isolates, four <i>C. fabianii</i> strains demonstrated the ability to enhance the overall flavor profile, particularly by increasing esters and chlorophyll degradation products. Additionally, several strains, particularly F3 and F4, also reduced total alkaloid and nitrogen content. Among these, <i>C. fabianii</i> strain F4 exhibited the most significant improvements. Sensory evaluation revealed that strain F4 could enhance mellowness, richness and sweetness while reducing irritation and off-flavors in fermented cigar tobacco leaves.</p><p><strong>Discussion: </strong>Our findings indicate that <i>C. fabianii</i> strain F4 can significantly improve the overall quality of cigar tobacco leaves through fermentation. This study provides a promising microbial fermentation agent for potential application in the cigar industry.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1492042"},"PeriodicalIF":4.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11666510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chicken cecal microbiome alters bile acids and riboflavin metabolism that correlate with intramuscular fat content.","authors":"Xiaoxia Long, Fuping Zhang, Liqi Wang, Zhong Wang","doi":"10.3389/fmicb.2024.1494139","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1494139","url":null,"abstract":"<p><p>Intramuscular fat (IMF) is a key indicator of chicken meat quality and emerging studies have indicated that the gut microbiome plays a key role in animal fat deposition. However, the potential metabolic mechanism of gut microbiota affecting chicken IMF is still unclear. Fifty-one broiler chickens were collected to identify key cecal bacteria and serum metabolites related to chicken IMF and to explore possible metabolic mechanisms. The results showed that the IMF range of breast muscle of Guizhou local chicken was 1.65 to 4.59%. The complexity and stability of ecological network of cecal microbiota in low-IMF chickens were higher than those in high-IMF chickens. Cecal bacteria positively related to IMF were <i>Alistipes</i>, <i>Synergistes</i> and <i>Subdoligranulum</i>, and negatively related to IMF were <i>Eubacterium_brachy_</i>group, <i>unclassified_f_Lachnospiraceae</i>, <i>unclassified_f_Coriobacteriaceae</i>, <i>GCA-900066575</i>, <i>Faecalicoccus</i>, and so on. Bile acids, phosphatidylethanolamine (Pe) 32:1 and other metabolites were enriched in sera of high-IMF chickens versus low-IMF chickens while riboflavin was enriched in sera of low-IMF chickens. Correlation analysis indicated that specific bacteria including <i>Alistipes</i> promote deposition of IMF in chickens via bile acids while the <i>Eubacterium_brachy</i> group, and <i>Coriobacteriaceae</i> promoted formation of riboflavin, glufosinate, C10-dats (tentative), and cilastatin and were not conducive to the IMF deposition.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1494139"},"PeriodicalIF":4.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11667789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142884802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analysis, culturing optimization, and characterization of <i>Escherichia</i> bacteriophage OSYSP, previously studied as effective pathogen control on fresh produce.","authors":"Mustafa Yesil, En Huang, Xu Yang, Ahmed E Yousef","doi":"10.3389/fmicb.2024.1486333","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1486333","url":null,"abstract":"<p><p>Advances in bacteriophage genome sequencing and regulatory approvals of some bacteriophages in various applications have renewed interest in these antibacterial viruses as a potential solution to persistent food safety challenges. Here, we analyzed in depth the genome of the previously studied <i>Escherichia</i> bacteriophage OSYSP (phage OSYSP), revealed its application-related characteristics, and optimized its enumeration techniques for facilitating industrial implementation. We previously sequenced phage OSYSP genome completely by combining results from Illumina Miseq and Ion Torrent sequencing platforms and completing the remaining sequence gaps using PCR. Based on the genomics analysis completed herein, phage OSYSP was confirmed as an obligate lytic phage of the <i>Caudoviricetes</i> class. The genome encodes 81 proteins of identifiable functions, including two endolysins and 45 proteins that support host-independent DNA replication, transcription, and repair. Despite its similarities to T5-like phages, unique genome arrangements confirm phage OSYSP's novelty. The genomic analysis also confirmed the absence of DNA sequences encoding virulence or antibiotic resistance factors. For optimizing phage detection and quantification in the conventional plaque assay, it was observed that decreasing the concentration of agar or agarose, when used as a medium gelling agent, increased phage recovery (<i>p</i> < 0.05), but using agarose resulted in smaller plaque diameters (<i>p</i> < 0.05). Phage OSYSP inactivated pathogenic and non-pathogenic strains of <i>E. coli</i> and some <i>Salmonella enterica</i> serovars, with more pronounced effect against <i>E. coli</i> O157:H7. Phage titers remained fairly unchanged throughout a 24-month storage at 4°C. Incubation for 30 min at 4°C-47°C or pH 4-11 had no significant detrimental effect (<i>p</i> > 0.05) on phage infectivity. <i>In vitro</i> application of phage OSYSP against <i>E. coli</i> O157:H7 EDL933 decreased the pathogen's viable population by >5.7-log CFU/mL within 80 min, at a multiplicity of infection as low as 0.01. The favorable genome characteristics, combined with improved enumeration methodology, and the proven infectivity stability, make phage OSYSP a promising biocontrol agent against pathogenic <i>E. coli</i> for food or therapeutic applications.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1486333"},"PeriodicalIF":4.0,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The biogeography of gastrointestinal mucosal microbiota of beef cattle at harvest.","authors":"J Daniel Young, Lee J Pinnell, Cory A Wolfe, Enrique Doster, Robert Valeris-Chacin, Ty E Lawrence, John T Richeson, Paul S Morley","doi":"10.3389/fmicb.2024.1490882","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1490882","url":null,"abstract":"<p><strong>Introduction: </strong>The gastrointestinal microbiota profoundly influences the health and productivity of animals. This study aimed to characterize microbial community structures of the mouth, gastrointestinal tract (GIT), and feces of cattle.</p><p><strong>Methods: </strong>Samples were collected from 18 Akaushi crossbred steers at harvest from multiple locations, including the oral cavity, rumen, abomasum, duodenum, jejunum, ileum, cecum, spiral colon, distal colon, and feces. These cattle were raised without exposure to antimicrobial drugs or hormone implants. Total microbial abundance was assessed using qPCR targeting the V3-V4 region of the 16S rRNA gene, and microbial community composition was evaluated through 16S rRNA gene sequencing.</p><p><strong>Results: </strong>Total microbial abundance was lesser in the small intestine than in other GIT regions (<i>p</i> ≤ 0.05). Additionally, microbial communities in the small intestine had lower richness and diversity than other regions (<i>p</i> ≤ 0.05). Microbial community compositions were measurably different along the GIT, with greater relatedness in adjacent GIT sections when progressing from oral to aboral locations. Firmicutes, Bacteroidota, and Actinobacteria were the dominant phyla in all samples. However, variations in composition were evident at lower taxonomic levels within these dominant phyla among samples from different regions. Genera previously associated with healthy gut microbiome communities were observed in low abundance across GIT regions. Taxa historically associated with liver abscesses (e.g., <i>Fusobacterium</i> and <i>Trueperella</i>) were detected in low abundance (≤0.02% relative abundance) throughout the GIT. In contrast, <i>Bacteroides</i>, which recently has been identified as a dominant feature in many liver abscesses, was observed in greater relative abundance (5.2% on average) in the hindgut.</p><p><strong>Discussion: </strong>This study provides an in-depth evaluation of the GIT of harvest-ready Akaushi crossbred cattle of varying growth rates. Clear differences exist in the abundance and composition of microbial populations at different points of the GIT. Unfortunately, no single GIT location can adequately represent the microbial communities of the entire GIT, which has important implications for future research. Additionally, examining microbiome data only at the phylum level likely oversimplifies important complexities of the microbial community structures, and investigations of lower taxonomic ranks should be included.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1490882"},"PeriodicalIF":4.0,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-enhanced assessment of potential probiotics from healthy calves for the treatment of neonatal calf diarrhea.","authors":"Yuting Zhai, Miju Kim, Peixin Fan, Sharath Rajeev, Sun Ae Kim, J Danny Driver, Klibs N Galvão, Christina Boucher, Kwangcheol C Jeong","doi":"10.3389/fmicb.2024.1507537","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1507537","url":null,"abstract":"<p><p>Neonatal calf diarrhea (NCD) remains a significant contributor to calf mortality within the first 3 weeks of life, prompting widespread antibiotic use with associated concerns about antimicrobial resistance and disruption of the calf gut microbiota. Recent research exploring NCD treatments targeting gut microbiota dysbiosis has highlighted probiotic supplementation as a promising and safe strategy for gut homeostasis. However, varying treatment outcomes across studies suggest the need for efficient treatment options. In this study, we evaluated the potential of probiotics <i>Limosilactobacillus reuteri</i>, formally known as <i>Lactobacillus reuteri</i>, isolated from healthy neonatal calves to treat NCD. Through <i>in silico</i> whole genome analysis and <i>in vitro</i> assays, we identified nine <i>L. reuteri</i> strains, which were then administered to calves with NCD. Calves treated with <i>L. reuteri</i> strains shed healthy feces and demonstrated restored gut microbiota and normal animal behavior. Leveraging a machine learning model, we evaluated microbiota profiles and identified bacterial taxa associated with calf gut health that were elevated by <i>L. reuteri</i> administration. These findings represent a crucial advancement towards sustainable antibiotic alternatives for managing NCD, contributing significantly to global efforts in mitigating antimicrobial resistance and promoting overall animal health and welfare.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1507537"},"PeriodicalIF":4.0,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric and visual analysis of human microbiome-breast cancer interactions: current insights and future directions.","authors":"Yi Zhou, Min Jiang, Xiaoyu Li, Ke Shen, Hui Zong, Qing Lv, Bairong Shen","doi":"10.3389/fmicb.2024.1490007","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1490007","url":null,"abstract":"<p><p>The composition of the gut microbiome differs from that of healthy individuals and is closely linked to the progression and development of breast cancer. Recent studies have increasingly examined the relationship between microbial communities and breast cancer. This study analyzed the research landscape of microbiome and breast cancer, focusing on 736 qualified publications from the Web of Science Core Collection (WoSCC). Publications in this field are on the rise, with the United States leading in contributions, followed by China and Italy. Despite this strong output, the centrality value of China in this field is comparatively low at ninth, highlighting a gap between the quantity of research and its global impact. This pattern is repetitively observed in institutional contributions, with a predominance of Western institutes among the top contributors, underscoring a potential research quality gap in China. Keyword analysis reveals that research hotspots are focused on the effect of microbiome on breast cancer pathogenesis and tumor metabolism, with risk factors and metabolic pathways being the most interesting areas. Publications point to a shift toward anti-tumor therapies and personalized medicine, with clusters such as \"anti-tumor\" and \"potential regulatory agent\" gaining prominence. Additionally, intratumor bacteria studies have emerged as a new area of significant interest, reflecting a new direction in research. The University of Helsinki and Adlercreutz H are influential institutions and researchers in this field. Current trends in microbiome and breast cancer research indicate a significant shift toward therapeutic applications and personalized medicine. Strengthening international collaborations and focusing on research quality is crucial for advancing microbiome and breast cancer research.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1490007"},"PeriodicalIF":4.0,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}