Frontiers in Microbiology最新文献

{"title":"Dynamic succession of the quantity and composition of epiphytic microorganisms at different growth stages on rice surface.","authors":"Lijuan Huang, Zhifei Zhang, Lin Mu, Xiong Liu, Rongji Sun, Wenjing Gao, Guihua Chen","doi":"10.3389/fmicb.2024.1451935","DOIUrl":"10.3389/fmicb.2024.1451935","url":null,"abstract":"<p><p>The quality of silage is uneven, which is due to the difference of epiphytic microorganisms of raw materials. To improve the quality of fermentation, the quantity and composition of epiphytic microorganisms are usually analyzed to better prepare silage. In this research, plate coating method and 16S high-throughput sequencing were used to analyze the differences in the quantity and composition of rice epiphytic microorganisms during different growth stages. The Lactic acid bacteria (LAB) and aerobic bacteria were the highest at the flowering stage, the yeast was the highest at the milk ripening stage, and the mould was the highest at the full ripening stage. And the growth stage also had a great influence on the composition of epiphytic bacterial community, at the phylum level, it was mainly composed of Proteobacteria. And at the genus level, <i>Pantoea, Acinetobacter, Pseudomonas</i> and <i>Chryseobacterium</i> were dominanted at the flowering stage; <i>Pantoea, Stenotrophomonas</i> and <i>Sphingobacterium</i> were dominanted at the milk ripening stage; <i>Acinetobacter, Pantoea, Chryseobacterium</i> and <i>Lactococcus</i> were dominanted at the dough stage; <i>Acinetobacter</i> and <i>Klebsiella</i> were dominated at the full ripening stage. Overall, the growth stage significantly affected the quantity and composition of rice epiphytic microorganisms. Therefore, rice silage can be modulated reasonably according to the number and composition of epiphytic microorganisms in different growth stages.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1451935"},"PeriodicalIF":4.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The microbiome analysis of ripen grape berries supports the complex etiology of sour rot.","authors":"Chiara Brischetto, Vittorio Rossi, Giorgia Fedele","doi":"10.3389/fmicb.2024.1450443","DOIUrl":"10.3389/fmicb.2024.1450443","url":null,"abstract":"<p><p>Sour rot (SR) is a grapevine disease complex that is not completely understood in its etiology and epidemiology. Recently, SR has received special attention due to its increasing economic importance due to crop losses and reduced wine quality. In this study, the fungal and bacterial microbiota of healthy (i.e., without rot symptoms) and rotten (i.e., exhibiting visual and olfactory SR symptoms) ripe bunches were characterized across 47 epidemics (39 vineyards in six Italian grape-growing areas) over three years. The 16S rRNA gene, ITS high-throughput amplicon sequencing, and quantitative PCR were used to assess the relative abundance and dynamic changes of microorganisms associated with SR. The estimators of genera richness of fungal communities within samples indicated a significantly different diversity between healthy and rotten bunches. For bacterial communities, the healthy and rotten bunches significantly differed in the total number of species, but not in abundance distribution across species. The bunch status (i.e., healthy and rotten) was a significant source of diversity (<i>p</i> < 0.01) when the community composition between samples was evaluated, indicating that microbiome composition varied between healthy and rotten bunches. In particular, healthy and rotten bunches shared 43.1 and 54.8% of fungal and bacterial genera, respectively; 31.3% (fungal) and 26.2% (bacterial) genera were associated with rotten bunches only. The yeast genera <i>Zygosaccharomyces</i>, <i>Zygoascus</i>, <i>Saccharomycopsis</i>, <i>Issatchenkia</i>, and <i>Pichia</i> and the bacterial genera <i>Orbus</i>, <i>Gluconobacter</i>, <i>Komagataeibacter</i>, <i>Gluconacetobacter</i>, and <i>Wolbachia</i> were strongly associated with bunches showing SR symptoms based on a linear discriminant analysis. These microorganisms have been associated with <i>Drosophila</i> insects in literature. The relationships between the microflora associated with SR-affected bunches and the roles of <i>Drosophila</i> in SR development need further investigation, which may open perspectives for more effective disease control.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1450443"},"PeriodicalIF":4.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Banxia-Yiyiren alleviates insomnia and anxiety by regulating the gut microbiota and metabolites of PCPA-induced insomnia model rats.","authors":"Liang Wang, Xiaorong Qi, Shuo Wang, Chujiao Tian, Tao Zou, Zihan Liu, Qi Chen, Yingfan Chen, Yunshan Zhao, Shaodan Li, Minghui Yang, Ningli Chai","doi":"10.3389/fmicb.2024.1405566","DOIUrl":"10.3389/fmicb.2024.1405566","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to clearly define the effects of Banxia-Yiyiren on the gut microbiota and its metabolites in a para-chlorophenylalanine-induced insomnia model and the possible underlying mechanisms involved.</p><p><strong>Materials and methods: </strong>We employed 16S ribosomal ribonucleic acid (rRNA) gene sequencing combined with metabonomic analysis to explore the mutual effects of the PCPA-induced insomnia model and the gut microbiota and the intrinsic regulatory mechanism of Banxia-Yiyiren on the gut microbiota and metabolites in the PCPA-induced insomnia model.</p><p><strong>Results: </strong>Banxia-Yiyiren was identified by mass spectrometry to include amino acids, small peptides, nucleotides, organic acids, flavonoids, fatty acids, lipids, and other main compound components. The elevated plus maze (EPM) test results revealed that high-dose Banxia-Yiyiren may increase willingness to explore by improving anxiety-like symptoms caused by insomnia. Through 16S rRNA gene sequencing, at the phylum level, compared with those in G1, the relative abundances of <i>Bacteroidota</i> and <i>Proteobacteria</i> in G2 increased, whereas the relative abundance of <i>Firmicutes</i> decreased. At the genus level, compared with those in G1, the relative abundances of <i>Prevotella_9</i>, <i>Prevotella</i>, <i>Ralstonia</i>, <i>Escherichia-Shigella</i>, and <i>UCG-005</i> in G2 increased, whereas the relative abundances of <i>Lactobacillus</i>, <i>Ligilactobacillus</i>, <i>Alloprevotella</i>, <i>Blautia</i>, and <i>Prevotellaceae_NK3B31_group</i> decreased. The metabolomics analysis results revealed 1,574 metabolites, 36.48% of which were classified as lipids and lipid-like molecules, 20.76% as organic acids and their derivatives, and 13.36% as organic heterocyclic compounds. The correlation between the top 20 differentially abundant metabolites in the G1-G2 groups was greater than that between the G3-G2 and G6-G2 groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the main differentially abundant metabolites in each group were significantly enriched in various pathways, such as amino acid metabolism, adenosine triphosphate (ATP)-binding cassette (ABC) transporters, protein digestion, and absorption. Additionally, there was a significant Pearson correlation between the genus-level differences in the gut microbiota and the differentially abundant metabolites among the G1-G2, G3-G2, and G6-G2 groups.</p><p><strong>Conclusion: </strong>This study preliminarily verified that the PCPA-induced insomnia model is closely related to gut microbial metabolism and microecological disorders, and for the first time, we confirmed that Banxia-Yiyiren can act on the gut microbiota of PCPA-induced insomnia model rats and alleviate insomnia and anxiety by regulating the species, structure, abundance, and metabolites of the gut microbiota.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1405566"},"PeriodicalIF":4.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: New therapeutic strategies against carbapenem-resistant gram-negative bacteria.","authors":"Maria Teresa Mascellino, Alessandra Oliva, Silpak Biswas, Giancarlo Ceccarelli","doi":"10.3389/fmicb.2024.1513900","DOIUrl":"10.3389/fmicb.2024.1513900","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1513900"},"PeriodicalIF":4.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrence and propagation of past functions through mineral facilitated horizontal gene transfer.","authors":"Taru Verma, Saghar Hendiani, Carlota Carbajo, Sandra B Andersen, Emma U Hammarlund, Mette Burmølle, Karina K Sand","doi":"10.3389/fmicb.2024.1449094","DOIUrl":"10.3389/fmicb.2024.1449094","url":null,"abstract":"<p><p>Horizontal gene transfer is one of the most important drivers of bacterial evolution. Transformation by uptake of extracellular DNA is traditionally not considered to be an effective mode of gene acquisition, simply because extracellular DNA is degraded in a matter of days when it is suspended in e.g. seawater. Recently the age span of stored DNA was increased to at least 2 Ma. Here, we show that <i>Acinetobacter baylyi</i> can incorporate 60 bp DNA fragments adsorbed to common sedimentary minerals and that the transformation frequencies scale with mineral surface properties. Our work highlights that ancient environmental DNA can fuel the evolution of contemporary bacteria. In contrast to heritable stochastic mutations, the processes by which bacteria acquire new genomic material during times of increased stress and needs, indicate a non-random mechanism that may propel evolution in a non-stochastic manner.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1449094"},"PeriodicalIF":4.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomic characterization of <i>Cellulosimicrobium funkei</i> isolate RVMD1 from Ma'an desert rock varnish challenges <i>Cellulosimicrobium</i> systematics.","authors":"Sulaiman M Alnaimat, Saqr Abushattal, Saif M Dmour","doi":"10.3389/fmicb.2024.1445943","DOIUrl":"10.3389/fmicb.2024.1445943","url":null,"abstract":"<p><p>Desert environments harbor unique microbial communities. This study focuses on <i>Cellulosimicrobium funkei</i> isolate RVMD1, isolated from rock varnish in the Ma'an Desert. Initial identification was achieved using 16S rRNA gene sequencing, followed by whole-genome sequencing (WGS) for comprehensive characterization. The genome comprises 4,264,015 base pairs (857 contigs) with a high G + C content of 74.59%. A total of 4,449 proteins were predicted. Comparative analysis utilizing OrthoANI, ANI, AAI, and dDDH metrics suggests that RVMD1 belongs to the <i>C. cellulans</i> group, with the highest similarity to <i>C. funkei</i> (97.71% ANI). Phylogenomic analysis of 43 <i>Cellulosimicrobium</i> genomes revealed significant heterogeneity within the genus. Our results challenge current systematics, with <i>C. cellulans</i> potentially representing up to 9 distinct genomospecies. Isolate RVMD1 shows genetic adaptations to its desert environment, including genes for denitrification, oxygen and sulfur cycling, and diverse hydrogen metabolism. Pangenomic analysis uncovered a considerable number of unique genes within RVMD1, highlighting its genetic distinctiveness. Gene family expansions suggest evolution in response to stressors like UV radiation and nutrient limitation. This study represents the first whole-genome analysis of a bacterium isolated from Jordanian rock varnish, emphasizing the value of WGS in understanding microbial diversity and adaptation in extreme environments.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1445943"},"PeriodicalIF":4.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of methanotrophic communities in atmospheric methane oxidation in paddy soils.","authors":"Yan Zheng, Yuanfeng Cai, Zhongjun Jia","doi":"10.3389/fmicb.2024.1481044","DOIUrl":"10.3389/fmicb.2024.1481044","url":null,"abstract":"<p><p>Wetland systems are known methane (CH₄) sources. However, flooded rice fields are periodically drained. The paddy soils can absorb atmospheric CH₄ during the dry seasons due to high-affinity methane-oxidizing bacteria (methanotroph). Atmospheric CH₄ uptake can be induced during the low-affinity oxidation of high-concentration CH₄ in paddy soils. Multiple interacting factors control atmospheric CH₄ uptake in soil ecosystems. Broader biogeographical data are required to refine our understanding of the biotic and abiotic factors related to atmospheric CH₄ uptake in paddy soils. Thus, here, we aimed to assess the high-affinity CH₄ oxidation activity and explored the community composition of active atmospheric methanotrophs in nine geographically distinct Chinese paddy soils. Our findings demonstrated that high-affinity oxidation of 1.86 parts per million by volume (ppmv) CH₄ was quickly induced after 10,000 ppmv high-concentration CH₄ consumption by conventional methanotrophs. The ratios of 16S rRNA to rRNA genes (rDNA) for type II methanotrophs were higher than those for type I methanotrophs in all acid-neutral soils (excluding the alkaline soil) with high-affinity CH₄ oxidation activity. Both the 16S rRNA:rDNA ratios of type II methanotrophs and the abundance of ¹³C-labeled type II methanotrophs positively correlated with high-affinity CH₄ oxidation activity. Soil abiotic factors can regulate methanotrophic community composition and atmospheric CH₄ uptake in paddy soils. High-affinity methane oxidation activity, as well as the abundance of type II methanotroph, negatively correlated with soil pH, while they positively correlated with soil nutrient availability (soil organic carbon, total nitrogen, and ammonium-nitrogen). Our results indicate the importance of type II methanotrophs and abiotic factors in atmospheric CH₄ uptake in paddy soils. Our findings offer a broader biogeographical perspective on atmospheric CH₄ uptake in paddy soils. This provides evidence that periodically drained paddy fields can serve as the dry-season CH₄ sink. This study is anticipated to help in determining and devising greenhouse gas mitigation strategies through effective farm management in paddy fields.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1481044"},"PeriodicalIF":4.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring phyllosphere fungal communities of 29 alpine meadow plant species: composition, structure, function, and implications for plant fungal diseases.","authors":"Fengzhen Yang, Xiaojian Pu, Cory Matthew, Zhibiao Nan, Xinrong Li","doi":"10.3389/fmicb.2024.1451531","DOIUrl":"10.3389/fmicb.2024.1451531","url":null,"abstract":"<p><p>The phyllosphere of plants hosts diverse fungal microbial communities. Despite the significant impact of plant fungal diseases on productivity and community ecology, the relationship between phyllosphere fungal communities and plant health in natural environments remains poorly understood. This study utilized high-throughput sequencing and field investigations to explore the composition, dynamics, and incidence of fungal diseases across 29 plant species from four functional groups (forbs, grasses, legumes, and sedges) in alpine meadow plant communities of the Qinghai-Tibetan Plateau. We identified Ascomycetes and Basidiomycetes as the predominant phyllosphere fungi. Significant differences were observed in the Shannon diversity index, <i>β</i>-diversity, indicator fungi, and hub fungi among the functional groups. With the exception of the sedge group, the incidence of fungal diseases in other groups was positively correlated with the proportion of pathogens in the phyllosphere fungal community. Predictive analyses revealed that <i>Ascochyta</i> was strongly associated with high disease incidence in grasses, <i>Cercospora</i> in forbs, and <i>Podosphaera</i> in legumes, while <i>Calophoma</i> was associated with low disease incidence in sedges. These findings enhance our understanding of how plant phyllosphere fungal communities assemble in natural environments and improve our ability to predict and manage foliar fungal diseases in alpine meadows.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1451531"},"PeriodicalIF":4.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11576429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A transformer-based deep learning model for identifying the occurrence of acute hematogenous osteomyelitis and predicting blood culture results.","authors":"Yingtu Xia, Qiang Kang, Yi Gao, Jiuhui Su","doi":"10.3389/fmicb.2024.1495709","DOIUrl":"10.3389/fmicb.2024.1495709","url":null,"abstract":"<p><strong>Background: </strong>Acute hematogenous osteomyelitis is the most common form of osteomyelitis in children. In recent years, the incidence of osteomyelitis has been steadily increasing. For pediatric patients, clearly describing their symptoms can be quite challenging, which often necessitates the use of complex diagnostic methods, such as radiology. For those who have been diagnosed, the ability to culture the pathogenic bacteria significantly affects their treatment plan.</p><p><strong>Method: </strong>A total of 634 patients under the age of 18 were included, and the correlation between laboratory indicators and osteomyelitis, as well as several diagnoses often confused with osteomyelitis, was analyzed. Based on this, a Transformer-based deep learning model was developed to identify osteomyelitis patients. Subsequently, the correlation between laboratory indicators and the length of hospital stay for osteomyelitis patients was examined. Finally, the correlation between the successful cultivation of pathogenic bacteria and laboratory indicators in osteomyelitis patients was analyzed, and a deep learning model was established for prediction.</p><p><strong>Result: </strong>The laboratory indicators of patients are correlated with the presence of acute hematogenous osteomyelitis, and the deep learning model developed based on this correlation can effectively identify patients with acute hematogenous osteomyelitis. The laboratory indicators of patients with acute hematogenous osteomyelitis can partially reflect their length of hospital stay. Although most laboratory indicators lack a direct correlation with the ability to culture pathogenic bacteria in patients with acute hematogenous osteomyelitis, our model can still predict whether the bacteria can be successfully cultured.</p><p><strong>Conclusion: </strong>Laboratory indicators, as easily accessible medical information, can identify osteomyelitis in pediatric patients. They can also predict whether pathogenic bacteria can be successfully cultured, regardless of whether the patient has received antibiotics beforehand. This not only simplifies the diagnostic process for pediatricians but also provides a basis for deciding whether to use empirical antibiotic therapy or discontinue treatment for blood cultures.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1495709"},"PeriodicalIF":4.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of wheat dwarf virus isolates from Serbia based on complete genome sequences.","authors":"Ivana Stanković, Katarina Zečević, Danijela Ristić, Ivan Vučurović, Branka Krstić","doi":"10.3389/fmicb.2024.1469453","DOIUrl":"10.3389/fmicb.2024.1469453","url":null,"abstract":"<p><strong>Introduction: </strong>Wheat dwarf virus (WDV), the species <i>Mastrevirus hordei</i> of the genus <i>Mastrevirus</i> in the family <i>Geminiviridae</i>, is a cereal virus commonly detected in several European, African and Asian countries that causes economic losses.</p><p><strong>Methods: </strong>In the spring of 2019, a severe outbreak of wheat dwarfing and yellowing was observed in many winter wheat crops across Serbia. A total of 161 samples were tested for the presence of WDV and other common wheat viruses using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). To obtain the complete genome of 23 selected isolates, several overlapping segments of the WDV genome were amplified and sequenced. A phylogenetic tree was constructed using the whole genome sequences of the WDV isolates identified in this study and 40 selected sequences from GenBank.</p><p><strong>Results and discussion: </strong>The results of DAS-ELISA indicated the presence of WDV in all samples collected from 21 sites in all nine districts surveyed. Further molecular characterization based on complete genome sequencing of 23 selected isolates showed that the Serbian WDV isolates had low nucleotide diversity and were closely related to wheat-infecting isolates from Europe, suggesting the presence of wheat-adapted forms of WDV in Serbia. The constructed phylogenetic tree revealed that Serbian isolates grouped in clade E within the wheat-adapted forms. This study provided the first insight into the genetic structure of WDV in Serbia based on its whole genome sequence. Further studies on the vector biology and population dynamics are needed to better understand the factors influencing the emergence and spread of WDV under local agroecological conditions.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1469453"},"PeriodicalIF":4.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11576430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}