{"title":"Characterization of <i>bla</i> <sub>NDM-5</sub>-carrying plasmids in two clinical <i>Salmonella</i> isolates from Jiaxing city, China.","authors":"Ping Li, Yongjuan Yuan, Yong Yan, Miaomiao Jia, Lei Gao, Xuejuan Liu, Yangming Sun, Guoying Zhu, Zhongwen Chen","doi":"10.3389/fmicb.2025.1620907","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong><i>Salmonella</i> is an important cause of foodborne diarrheal diseases worldwide. The emergence of <i>bla</i> <sub>NDM</sub>-positive carbapenem-resistant <i>Salmonella enterica</i> isolates in recent years poses a huge public health challenge.</p><p><strong>Methods: </strong>In this study, two clinical <i>S. enterica</i> isolates carrying <i>bla</i> <sub>NDM-5</sub>: a serotype 4,[5],12:i:- strain (2023JX045) and a serovar Stanley strain (2024-406) were analyzed using antimicrobial susceptibility testing and whole genome sequencing.</p><p><strong>Results: </strong>Both isolates were multidrug resistant, with insusceptibility to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, cefuroxime, ceftiofur, cefazolin, cefoxitin, cefotaxime, ceftazidime, cefepime, ceftazidime/avibactam, meropenem, imipenem, ertapenem, tetracycline, gentamicin, trimethoprim/sulfamethoxazole, florfenicol, chloramphenicol, ciprofloxacin, colistin, and polymixin B. The <i>bla</i> <sub>NDM-5</sub>-carrying plasmids in 2023JX045 and 2024-406 were named p23045-NDM5 and p2024406-NDM5, respectively, with both belonging to the incompatibility (Inc)HI2/IncHI2A group and sequence type ST3. In 2023JX045, <i>bla</i> <sub>NDM-5</sub> was flanked by two same-oriented copies of IS<i>26</i> elements (IS<i>26-dsbC-trpF</i>-<i>ble</i> <sub>MBL</sub>-<i>bla</i> <sub>NDM-5</sub>-IS<i>5</i>-ΔIS<i>3000</i>-ΔIS<i>Kox3-umuC-umuD</i>-IS<i>26</i>). In 2024-406, p2024406-NDM5 was found to carry two copies of <i>bla</i> <sub>NDM-5</sub>, possibly resulting from duplication of IS<i>26</i>-<i>dsbC-trpF</i>-<i>ble</i> <sub>MBL</sub>-<i>bla</i> <sub>NDM-5</sub>-IS5-ΔISAba125-ΔIS3000-ΔISKox3-umuC-umuD-IS26 and interrupted by mobile element IS<i>1</i> at the upstream region of ΔIS3000.</p><p><strong>Discussion: </strong>This is the first report to describe the presence of two <i>bla</i> <sub>NDM-5</sub> copies on an IncHI2/IncHI2A plasmid carried by serovar Stanley, as well as the dissemination of <i>bla</i> <sub>NDM-5</sub> in Salmonella in Jiaxing City, China. IS26-flanked composite transposons appeared to play an important role in the formation of this region. The dissemination of blaNDM in Salmonella isolates and the complexity of the <i>bla</i> <sub>NDM-5</sub> region highlight the urgent need to monitor carbapenem-resistant <i>S. enterica</i>.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1620907"},"PeriodicalIF":4.0000,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12240926/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fmicb.2025.1620907","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction: Salmonella is an important cause of foodborne diarrheal diseases worldwide. The emergence of blaNDM-positive carbapenem-resistant Salmonella enterica isolates in recent years poses a huge public health challenge.
Methods: In this study, two clinical S. enterica isolates carrying blaNDM-5: a serotype 4,[5],12:i:- strain (2023JX045) and a serovar Stanley strain (2024-406) were analyzed using antimicrobial susceptibility testing and whole genome sequencing.
Results: Both isolates were multidrug resistant, with insusceptibility to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, cefuroxime, ceftiofur, cefazolin, cefoxitin, cefotaxime, ceftazidime, cefepime, ceftazidime/avibactam, meropenem, imipenem, ertapenem, tetracycline, gentamicin, trimethoprim/sulfamethoxazole, florfenicol, chloramphenicol, ciprofloxacin, colistin, and polymixin B. The blaNDM-5-carrying plasmids in 2023JX045 and 2024-406 were named p23045-NDM5 and p2024406-NDM5, respectively, with both belonging to the incompatibility (Inc)HI2/IncHI2A group and sequence type ST3. In 2023JX045, blaNDM-5 was flanked by two same-oriented copies of IS26 elements (IS26-dsbC-trpF-bleMBL-blaNDM-5-IS5-ΔIS3000-ΔISKox3-umuC-umuD-IS26). In 2024-406, p2024406-NDM5 was found to carry two copies of blaNDM-5, possibly resulting from duplication of IS26-dsbC-trpF-bleMBL-blaNDM-5-IS5-ΔISAba125-ΔIS3000-ΔISKox3-umuC-umuD-IS26 and interrupted by mobile element IS1 at the upstream region of ΔIS3000.
Discussion: This is the first report to describe the presence of two blaNDM-5 copies on an IncHI2/IncHI2A plasmid carried by serovar Stanley, as well as the dissemination of blaNDM-5 in Salmonella in Jiaxing City, China. IS26-flanked composite transposons appeared to play an important role in the formation of this region. The dissemination of blaNDM in Salmonella isolates and the complexity of the blaNDM-5 region highlight the urgent need to monitor carbapenem-resistant S. enterica.
期刊介绍：
Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
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