Frontiers in Microbiology最新文献

{"title":"Editorial: Deep subsurface microbiology and energetics.","authors":"Alberto Robador, William J Brazelton, James Andrew Bradley","doi":"10.3389/fmicb.2025.1611707","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1611707","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1611707"},"PeriodicalIF":4.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12093771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology and resistance mechanisms of tigecycline- and carbapenem-resistant <i>Enterobacteriaceae</i> in China: a multicentre genome-based study.","authors":"Lixin Yan, Tingting Ma, Wen Wang, Zhen Cai, Hong Du, Zhongju Chen, Renru Han, Yan Guo, Gang Li, Wei Jia, Jia Tao","doi":"10.3389/fmicb.2025.1582851","DOIUrl":"10.3389/fmicb.2025.1582851","url":null,"abstract":"<p><strong>Objectives: </strong>To elucidate the molecular epidemiology of tigecycline and carbapenem-resistant <i>Enterobacteriaceae</i> isolates and mechanisms of tigecycline resistance.</p><p><strong>Methods: </strong>We gathered 31 unduplicated strains of tigecycline-resistant <i>Enterobacteriaceae</i> from six hospitals nationwide. Antimicrobial susceptibility testing, phenotypic detection, and PCR identification were performed first, followed by homology analysis using MLST and PFGE. Conjugation transfer experiments using resistance gene plasmids were carried out, and the conjugates' growth curves were examined. All strains were sequenced using the Illumina HiSeq technology, and we identified a strain KP28 carrying a complete gene cluster <i>tmexCD2-toprJ2</i>. Then, its plasmid was further constructed using the PacBio platforms to complete the frame. The genetic connection of the <i>tmexCD2-toprJ2</i> gene cluster carried by KP28 was established using core genome analyses.</p><p><strong>Results: </strong>All 31 tigecycline-resistant <i>Enterobacteriaceae</i> strains (TG-CRE) were multidrug resistant. PFGE classified strains of CRKP, CRECL, and CRKAE into 16 distinct spectra, 6 distinct spectra, and 3 distinct spectra. MLST results showed a high concentration of ST11 in CRKP strains and a predominance of ST116 in CRECL strains, suggesting possible clonal transmission or selective dominance. The findings of the plasmid conjugation assay revealed that three strains expressing carbapenem resistance genes were effectively transmitted to the recipient cell <i>E. coli</i> EC600. WGS data revealed that these 31 strains include 79 resistance genes, with one strain (KP28) carrying the whole tigecycline resistance gene cluster, <i>tmexC2D2-toprJ2</i>. This resistance gene is contained in a large IncHI5 plasmid, which is difficult to transfer.</p><p><strong>Conclusion: </strong>The overall carriage rate of the <i>tmexC2D2-toprJ2</i> gene cluster was found to be low among the five Chinese hospitals investigated. Conversely, <i>tet</i>(A) mutations were present in most of the strains. Bacteria with the carbapenem resistance genes <i>bla</i> _KPC and <i>bla</i> _NDM are vulnerable to horizontal transmission. Increasing the risk of transmission of antibiotic-resistant genes.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1582851"},"PeriodicalIF":4.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term effects of thinning on the growth and soil improvement of typical stands in the Yellow River Delta.","authors":"Wenjing Liu, Lichao Wang, Jiangbao Xia, Yue Lu, Xianguo Zong","doi":"10.3389/fmicb.2025.1585176","DOIUrl":"10.3389/fmicb.2025.1585176","url":null,"abstract":"<p><p>The effects of thinning were studied on stand growth and the physicochemical soil characteristics of typical plantations in the Yellow River flood plain in northern Shandong Province. Growth indices and soil physical and chemical indices were monitored and in 8-year-old plantations of <i>Fraxinus chinensis</i>, <i>Salix matsudana</i>, and <i>Ulmus pumila</i> in the Yellow River Delta. Data were collected at an initial stand density of 3 m × 3 m and 3 years after artificial thinning to a stand density of 3 m × 6 m. (1) Thinning promoted growth, and the effect on the <i>U. pumila</i> plantation was the greatest. The diameter at breast height, tree height and crown width increased by 41.28, 19.98, and 59.49%, respectively. (2) Thinning increased the soil moisture at the studied plantations, reduced the soil bulk density, and increased the soil porosity and the water holding capacity. The results differed among the plantations, with the greatest improvement occurring at the <i>U. pumila</i> plantation. (3) Thinning had a greater effect on inorganic than on organic soil nutrients according to forest type; the changes in nitrogen, phosphorus and potassium contents in different soil layers substantially varied with the species planted. Most variables significantly differed at the <i>S. matsudana</i> plantation, and the changes in different soil layers were inconsistent. (4) Thinning significantly increased the correlations between stand growth and soil physicochemical properties. Thinning positively affected forest growth and soil physicochemical properties, but the variation in each index across the different plantations was quite different. Thus, thinning could help promote the stable and sustainable development of forest plantations in the Yellow River Delta, and these results provide a reference for the rational management of plantations.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1585176"},"PeriodicalIF":4.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological, clinical, and laboratory characteristics of 581 human brucellosis cases in Xinjiang, China.","authors":"Bin Luo, Qian Wang, Shuting Yang, Xue Song, Zhiwei Li","doi":"10.3389/fmicb.2025.1541277","DOIUrl":"10.3389/fmicb.2025.1541277","url":null,"abstract":"<p><strong>Background: </strong>Epidemiological and clinical analyses of brucellosis are crucial for the development of surveillance and case management strategies.</p><p><strong>Methods: </strong>We analyzed the epidemiological and clinical characteristics of 581 human brucellosis cases in Xinjiang. Demographic characteristics of patients with brucellosis and their clinical manifestations were collected and analyzed.</p><p><strong>Results: </strong>Among the 581 brucellosis patients, the men-to-women ratio was 2.8:1.0 (428:153); the age was (44.41 ± 16.25) years, ranging from 1 to 83 years, mainly concentrated in the 35-60 age group, accounting for 70.91% (412 cases); the ethnic distribution was mainly Uyghur, accounting for 50.60%; the occupational distribution was mainly farmers, accounting for 43.20%. A total of 186 patients had a clear history of contact with cattle and sheep breeding. Clinical staging was mainly chronic stage patients, accounting for 55.24% (321 cases), and there were 48 cases with complications, mainly pain and fatigue, accounting for 8.26%. The most common laboratory examination characteristics were increased erythrocyte sedimentation rate and increased C-reactive protein level, accounting for 29.09% and 23.06%, respectively, and the blood culture detection rate was low (4.48 %).</p><p><strong>Conclusion: </strong>Patients with brucellosis in the Xinjiang Uyghur Autonomous Region predominantly comprised middle-aged and young men primarily involved in farming. The principal clinical symptoms include pain and fever; however, the positivity rate of <i>Brucella</i> cultures in these patients is low. To minimize the risk of missed diagnoses or misdiagnoses, it is recommended to integrate epidemiological history, clinical manifestations, and laboratory examination results into the diagnostic process to facilitate earlier detection and treatment.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1541277"},"PeriodicalIF":4.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome sequencing reveals virulence-mobile element linkages and phylogenetic diversity in multidrug-resistant <i>Escherichia coli</i> from Nigeria.","authors":"Nubwa Medugu, Mabel Kamweli Aworh, Kenneth Iregbu, Philip Nwajiobi-Princewill, Dawn M Hull, Lyndy Harden, Pallavi Singh, Stephen Obaro, Abiodun Egwuenu, Faith Adeboye, Ruth Egah, Leonard Uzairue, Yahaya Mohammed, Nwafia Ifeyinwa, Siddhartha Thakur","doi":"10.3389/fmicb.2025.1579175","DOIUrl":"10.3389/fmicb.2025.1579175","url":null,"abstract":"<p><strong>Background: </strong>Multidrug-resistant <i>Escherichia coli</i> poses a critical public health threat in Nigeria, where limited genomic surveillance hinders the understanding of virulence-resistance interplay.</p><p><strong>Methods: </strong>This cross-sectional study employed whole-genome sequencing to characterize 107 MDR-E isolates from a Nigerian tertiary hospital (2019-2020), analyzing virulence genes, mobile genetic elements (MGEs), phylogroups, sequence types (STs), pathotypes, and antimicrobial resistance (AMR).</p><p><strong>Results: </strong>We identified 2,021 virulence genes across nine functional categories, dominated by immune evasion (<i>terC</i>, 96.3%), adherence (<i>fimH</i>, 86%), and iron acquisition (<i>fyuA</i>, 63.6%). Strikingly, 81.3% of virulence genes were linked to MGEs, including MITEEc1 (75.7% of isolates) and IS30 (56.1%), with IncFII (17.8%) and Col156 (12.1%) plasmids co-harboring virulence (e.g., <i>traJ/traT</i>, <i>senB</i>) and AMR genes (e.g., <i>blaTEM-1B</i>). Phylogroup B2 (32.7%) dominated, exhibiting high resistance to ampicillin (97.1%) and emerging meropenem resistance (11.4%). Globally disseminated STs (ST131, ST410, ST648) carried significantly more diverse virulence genes than minor clones (<i>p</i> = 0.028) and were strongly associated with double-serine QRDR mutations (<i>gyrA_S83L</i>: 97.6%, <i>parC_S80I</i>: 97.6%), which correlated with more virulence genes (24.2 vs. 22.3 genes) and resistance (MAR index: 0.7 vs. 0.5) compared to minor clones. Notably, 92% (61/66) of high-risk clones harbored these mutations, versus 57% (21/37) of low-risk clones, suggesting a fitness advantage enabling major clones to sustain larger genetic cargoes. Pathotyping revealed 54.2% as extraintestinal pathogenic <i>E. coli</i> (ExPEC), with 72.4% of these being uropathogenic (UPEC) and 5.2% ExPEC/EAEC hybrids, alongside 43.9% atypical ExPEC strains. Hierarchical clustering demonstrated phylogroup B2's genetic diversity and co-localization of plasmid-borne virulence/AMR genes.</p><p><strong>Discussion: </strong>These findings underscore Nigeria's MDR-E crisis, driven by MGE-facilitated gene transfer, hybrid pathotypes, and globally disseminated high-risk clones harboring double-serine QRDR mutations. There is continued need for robust genomic surveillance, stringent infection control measures, enhanced antibiotic stewardship, and exploration of antivirulence strategies (e.g., targeting <i>fimH</i> or <i>yeh</i>) to curb the spread of these highly adaptable pathogens in resource-limited settings and beyond.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1579175"},"PeriodicalIF":4.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomic insight into the myxobacterial carbohydrate-degrading potential.","authors":"Niharika Saraf, Gaurav Sharma","doi":"10.3389/fmicb.2025.1550287","DOIUrl":"10.3389/fmicb.2025.1550287","url":null,"abstract":"<p><p>Myxobacteria are an intriguing group of social-behavior-depicting microbes with unique physiological characteristics such as fruiting body formation, gliding motility, and predation, encompassing the largest genomes (>9 Mb) within the Eubacteria kingdom. These soil-dwelling organisms are crucial for lignocellulosic biomass degradation, which has both ecological and industrial significance. While previous studies have demonstrated polysaccharide deconstruction abilities in a few myxobacterial species, we aim to elucidate the distribution of their Carbohydrate Active Enzymes (CAZymes) domains per organism, with a focus on proteins involved in the catabolism of critical polysaccharides such as cellulose, lignin, xylan, starch, pectin, fructan, chitin, and dextran, across 61 high-quality sequenced myxobacterial genomes. Our findings reveal that 3.5% of the total genes at the median level have domains related to CAZyme functions across different myxobacterial families. Notably, family Archangiaceae (4.4%) and Myxococcaceae (3.7%) members exhibit the most significant genomic diversity and potential for degrading multiple substrates within lignocellulosic biomass. These plentiful CAZymes probably enable these majorly soil-harboring myxobacteria to breakdown various carbohydrate substrates into simpler biological molecules, which not only allow these organisms to sustain in poor-nutrient environments but also enable them to be critical players in carbon cycling and organic matter decomposition. Overall, it can be postulated that myxobacteria have an unexplored genomic potential that may play an integral role in the degradation of recalcitrant plant biomass, potentially influencing soil health and composition. This study further suggests the critical ecological importance of these CAZymes in sustaining the balance of terrestrial ecosystems and diverse industrial applications.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1550287"},"PeriodicalIF":4.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12093494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization, diversity, and biogeochemical potential of soil viruses inhabiting in Yuncheng Salt Lake.","authors":"Jin Liu, Xiaoxiao Dong, Xiaokai Wang, Yunmeng Chu","doi":"10.3389/fmicb.2025.1597514","DOIUrl":"10.3389/fmicb.2025.1597514","url":null,"abstract":"<p><p>Viruses play a crucial role in microbial communities and can significantly influence ecosystem processes and biogeochemical cycles by regulating the structure of these communities and the metabolic functions of their hosts. Salt lakes are recognized for harboring a diverse array of halotolerant microorganisms; however, there is limited knowledge regarding the viruses and their co-occurring hosts in these halotolerant environments. Herein, 3,362 viral operational taxonomic units (vOTUs) were recovered from Yuncheng Salt Lake soils by combining with a binning method, with less than 2% showing homology to known viruses, highlighting a significant unexplored viral diversity. Virus communities were typically specific to their habitats, exhibiting low overlap across various geographical regions and other ecological environments. It was predicted that 27.4% of viruses were associated with microbial host populations, and the majority of the predicted vOTUs are linked to dominant bacteria and archaea. Metabolic predictions revealed that 568 putative auxiliary metabolic genes (AMGs) were involved in various processes related to biosynthesis and transformation reactions associated with resource utilization within host cells. The virus-encoded AMGs indicated that these viruses influenced the metabolism of carbon, nitrogen, sulfur, and phosphorus in microorganisms, as well as their adaptation to salinity. This study highlighted the unique characteristics and potential ecological roles of soil viruses in Yuncheng Salt Lake, suggesting that these viruses may significantly influence microbial communities and the biogeochemistry of salt lake soils. These findings provide valuable insights into the diversity, function, and ecology of viruses in soils surrounding salt lakes, establishing a foundation for understanding their roles in these unique ecosystems.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1597514"},"PeriodicalIF":4.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold water swimming reshapes gut microbiome to improve high-fat diet-induced obesity.","authors":"Jie Men, Chenglong Cui, Hao Li, Zhaowei Li, Yu Zhang, Zhiyu Liu, Qi Wang, Penghong Liu, Shuangling Zou, Zhengyang Yu, Yuxi Zhang, Simin Wu, Guoyu Zhu, Pengbo Wang, Xiaoli Huang","doi":"10.3389/fmicb.2025.1589902","DOIUrl":"10.3389/fmicb.2025.1589902","url":null,"abstract":"<p><p>Hypothermia and swimming have been shown to alleviate high-fat diet (HFD)-induced obesity, with effects linked to the gut microbiota (GM). However, whether the effects of cold water swimming (CWS) on GM can be effectively transferred through fecal microbiota transplantation (FMT) has not been investigated. This study established mice models of obesity, CWS and FMT to investigate the mechanism by which CWS reshapes GM to improve HFD-induced obesity. Additionally, we analyzed the relationship between obesity phenotypes, GM composition, gene expression and CWS. The study found that HFD induced obesity phenotypes and GM dysbiosis in mice, while CWS produced opposite effects. The FMT results confirmed that CWS effectively alleviated HFD-induced lipid accumulation, metabolic disorders, and chronic inflammatory responses, which are associated with increased GM diversity, enrichment of beneficial bacteria, and the repair of intestinal barrier damage. Furthermore, these beneficial effects can be effectively transferred via FMT. The evidence from this study suggests that GM plays a critical role in the anti-obesity effects of CWS, with intestinal barrier repair emerging as a potential therapeutic target. This also provides scientific evidence for the feasibility of FMT as a strategy to combat obesity.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1589902"},"PeriodicalIF":4.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12093064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phage (cocktail)-antibiotic synergism: a new frontier in addressing <i>Klebsiella pneumoniae</i> resistance.","authors":"Chandrasekar Karthika, Nambiraman Malligarjunan, Nagaiah Hari Prasath, Shunmugiah Karutha Pandian, Shanmugaraj Gowrishankar","doi":"10.3389/fmicb.2025.1588472","DOIUrl":"10.3389/fmicb.2025.1588472","url":null,"abstract":"<p><p>Phages, which play a crucial role in regulating bacterial populations and evolution, have gained renewed attention as potential therapeutic agents especially in the face of rising antimicrobial resistance, such as in <i>Klebsiella pneumoniae</i>- a MDR pathogen with significant clinical implications for immunocompromised individuals. In this milieu, the present investigation aimed at evaluating the therapeutic potential of two lytic phages, KPKp (jumbo phage) and KSKp, as potential candidates for phage treatment. Initial purification and TEM characterization revealed their family as <i>Ackermannviridae</i> (KPKp) and <i>Straboviridae</i> (KSKp). The one-step growth curve analysis divulged that KPKp and KSKp exhibit burst sizes of ~98 and ~121 and latency periods of 8 and 12 min, respectively. Genomic analysis unveiled linear double-stranded DNA as their genome with sizes 206,819 bp (KPKp) and 167,101 bp (KSKp) lacking virulence or lysogenic genes, signifying their therapeutic suitability. Evaluation of phages as a cocktail demonstrated a substantial improvement in lytic ability, achieving complete (100%) lysis (at MOI 1) of clinical isolates compared to individual phages, achieving 50 and 25% lysis at MOI 1. <i>In vitro</i> investigations demonstrated that the phage cocktail significantly decreased both planktonic and sessile cells. Additionally, the phage (cocktail)-antibiotic synergism (PAS) achieves over 90% inhibition of <i>K. pneumoniae</i>, even at sub-lethal antibiotic doses. PAS treatment significantly prolongs the lifespan of <i>K. pneumoniae</i>-infected <i>Galleria mellonella</i>. Compared to cocktail phage therapy, PAS demonstrates a superior reduction in bacterial load. In conclusion, the combination of phages and antibiotic holds potential for addressing clinical challenges associated with MDR <i>K. pneumoniae</i> infection.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1588472"},"PeriodicalIF":4.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analysis of carbapenem-resistant <i>Klebsiella pneumoniae</i> blood isolates from nationwide surveillance in South Korea.","authors":"Younggwon On, Jung Wook Kim, Juyoung Lee, Jung Sik Yoo","doi":"10.3389/fmicb.2025.1562222","DOIUrl":"10.3389/fmicb.2025.1562222","url":null,"abstract":"<p><strong>Introduction: </strong>Carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP) poses a significant threat to public health owing to its multidrug resistance and rapid dissemination.</p><p><strong>Methods: </strong>This study analyzed CRKP isolates collected from bloodstream infections in nine regions of South Korea using the Kor-GLASS surveillance system between 2017 and 2021.</p><p><strong>Results: </strong>A total of 3,941 <i>K. pneumoniae</i> isolates were collected. Among them, 119 (3%) isolates were identified as CRKP. Most CRKP (79.7%) belonged to sequence type 307 (ST307), followed by ST11 (6.8%). All CRKP isolates exhibited multidrug resistance, with 78.8% carrying the IncX3 plasmid encoding the <i>KPC-2</i> gene. Phylogenetic and genomic analyses revealed that ST307 isolates exhibited low single nucleotide polymorphism (SNP) differences. SNP differences among ST307 strains ranged from a minimum of 1 to a maximum of 140, indicating close genetic relatedness. All ST307 strains harbored the KL102 and O1/O2v2 loci, and genomic analysis revealed high prevalence of key resistance genes such as <i>KPC</i> (91.5%) and <i>CTX-M-15</i> (83.9%), alongside mutations in the <i>QRDR</i> (ParC-80I, GyrA-83I) and <i>ompK</i> genes. Two major clusters were identified, with cluster 1 harboring yersiniabactin lineage 16 (ICEkp12) and cluster 2 showing higher virulence, including the yersiniabactin lineage 17 (ICEkp10) and colibactin-associated genes.</p><p><strong>Discussion: </strong>These findings underscore the dominance of ST307 among CRKP isolates in Korea, which is driven by clonal expansion and the critical role of mobile genetic elements. Therefore, enhanced genomic surveillance and targeted infection control measures are urgently required to address the spread of CRKP in clinical settings.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1562222"},"PeriodicalIF":4.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}