Frontiers in Microbiology最新文献

{"title":"Effects of γ-PGA application on soil physical and chemical properties, rhizosphere microbial community structure and metabolic function of urban abandoned land.","authors":"Tu Shiheng, Ge Fanglan, Xu Yong, Sun Qingyi, Xie Fugui, Ren Yao, Du Juan, Chu Can, Xu Boyuan, Li Wei","doi":"10.3389/fmicb.2025.1534505","DOIUrl":"10.3389/fmicb.2025.1534505","url":null,"abstract":"<p><strong>Introduction: </strong>China's rapid urbanization has led to the conversion of extensive farmland on urban fringes into non-grain uses, exacerbating the scarcity of arable land resources. Reclaiming these abandoned or underutilized areas presents a viable solution. However, many of these lands are contaminated with construction debris and have uneven soil quality, rendering them unsuitable for crop cultivation. This study aims to investigate the effects of γ-polyglutamic acid (γ-PGA) on improving such soils.</p><p><strong>Methods: </strong>A 6-month field experiment was conducted on green spaces with mixed construction waste in Chengdu's urban ring. The study analyzed the impact of γ-PGA on soil bacterial communities, metabolites, and physicochemical properties during different wheat growth stages, namely tillering, jointing, flowering, and maturity.</p><p><strong>Results: </strong>γ-PGA significantly increased soil organic matter, total nitrogen, nitrate nitrogen, and alkali-hydrolyzable nitrogen. It also boosted enzyme activities such as urease, sucrase, and alkaline phosphatase. The soil mechanical structure improved, with increases in clay, sand, and macroaggregates. As wheat grew, the fractal dimensions of soil volume and infiltration performance increased, while bulk density decreased, indicating enhanced water retention and gas exchange. Beneficial microorganisms like Actinobacteria and Devosia increased in abundance, promoting soil fertility. Metabolomics analysis revealed that γ-PGA enriched pathways involved in carbohydrate digestion, starch metabolism, and nucleotide processes, creating a more favorable environment for plant growth.</p><p><strong>Discussion: </strong>This research underscores the crucial role of γ-PGA in soil restoration and fertility enhancement. The findings provide valuable insights for reclaiming non - grain farmland, offering a potential solution to the challenge of arable land shortage caused by urbanization. The study's results contribute to the existing knowledge on soil improvement techniques and have practical implications for sustainable agricultural development in urbanized regions. However, further research could explore the long-term effects of γ-PGA application and its applicability in different soil types and environmental conditions.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1534505"},"PeriodicalIF":4.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of gene expression under high hydrostatic pressure: the versatile role of the master regulator SurR in energy metabolism.","authors":"Yann Moalic, Toan Bao Hung Nguyen, Jordan Hartunians, Tiphaine Birien, Axel Thiel, Mohamed Jebbar","doi":"10.3389/fmicb.2025.1593936","DOIUrl":"10.3389/fmicb.2025.1593936","url":null,"abstract":"<p><p>In <i>Thermococcales</i>, energy metabolism genes are regulated by the sulfur-responsive transcriptional regulator SurR. In the piezophilic archaeon <i>Thermococcus barophilus</i>, these genes are also influenced by hydrostatic pressure. To explore the interaction between pressure, sulfur availability, and gene regulation, we constructed and analyzed several deletion mutants, including a partial <i>surR</i> knockout, under varying conditions. Our results show that hydrostatic pressure modulates the expression of energy metabolism genes and that SurR is essential for activating the hydrogenogenic gene cluster, even in sulfur-rich environments. Under sulfur limitation, the membrane-bound sulfur-reducing complex (MBS) is required for normal growth. These regulatory patterns expand current models derived from non-piezophilic species such as <i>Pyrococcus furiosus</i> and <i>Thermococcus kodakarensis</i>. Overall, our findings indicate that hydrostatic pressure shapes SurR function in <i>T. barophilus</i>, reflecting its adaptive plasticity in extreme environments.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1593936"},"PeriodicalIF":4.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The inhibitory potential of three scorpion venom peptides against multidrug-resistant <i>Klebsiella pnemoniae</i>.","authors":"Rosa Giugliano, Roberta Della Marca, Annalisa Chianese, Alessandra Monti, Federica Donadio, Emanuela Esposito, Nunzianna Doti, Carla Zannella, Massimiliano Galdiero, Anna De Filippis","doi":"10.3389/fmicb.2025.1569719","DOIUrl":"10.3389/fmicb.2025.1569719","url":null,"abstract":"<p><strong>Background: </strong>The rise of drug-resistant bacterial infections, particularly those caused by <i>Klebsiella pneumoniae</i> underscores the urgent need for novel therapeutic candidates. Hospital-acquired infections from <i>K. pneumoniae</i> carbapenemase (KPC)-producing bacteria pose a serious health threat, particularly impacting the respiratory and urinary tracts. This study investigates the antibacterial efficacy of three antimicrobial peptides, called pantinins, derived from <i>Pandinus imperator</i> scorpions' venom against <i>K. pneumoniae</i> and various KPC-producing clinical isolates.</p><p><strong>Methods: </strong>Non-toxic concentrations were established for each peptide using MTT method. Antibacterial activity was performed through the liquid microdilution assay to assess the minimum inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC). A time-kill test was conducted by recording the colonies forming units (CFUs) at several times and compared with those of the untreated bacteria. Finally, the effect of peptides on the gene expression of virulence factors of <i>K. pneumoniae</i> was evaluated through qPCR.</p><p><strong>Results: </strong>Antibacterial assays demonstrated bactericidal effects for pantinin-1 and pantinin-2 within 1 h, while pantinin-3 displayed bacteriostatic properties. Noteworthy, MIC values ranged from 6 to 25 μM for <i>K. pneumoniae</i> and from 25 to 50 μM for KPC strains. Pantinins exert their antimicrobial effect through a membranolytic mechanism, directly interacting with outer membrane lipopolysaccharides (LPS), as evidenced by circular dichroism (CD) spectra and scanning electron microscopy (SEM). In addition, qPCR showed a reduction in virulence genes expression, suggesting the antibacterial effect of peptides also at transcriptional level.</p><p><strong>Conclusion: </strong>These findings support pantinins as promising candidates for treating multidrug-resistant <i>K. pneumoniae</i> infections.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1569719"},"PeriodicalIF":4.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential effect of <i>Wolbachia</i> on virus restriction in the spider mite <i>T. truncatus</i>.","authors":"Lucas Yago Melo Ferreira, João Pedro Nunes Santos, David Gabriel do Nascimento Souza, Lixsy Celeste Bernardez Orellana, Sabrina Ferreira de Santana, Anderson Gonçalves Sousa, Paula Luize Camargos Fonseca, Amanda Gabrielly Santana Silva, Vinicius Castro Santos, Isaque João da Silva de Faria, Roenick Proveti Olmo, Luis Gustavo Carvalho Pacheco, Marcio Gilberto Cardoso Costa, Carlos Priminho Pirovani, Anibal Ramadan Oliveira, Eric Roberto Guimarães Rocha Aguiar","doi":"10.3389/fmicb.2025.1570606","DOIUrl":"10.3389/fmicb.2025.1570606","url":null,"abstract":"<p><p>The mite <i>T. truncatus</i> is a significant agricultural pest and may serve as a potential vector for viral transmission. However, the virome of <i>T. truncatus</i> remains understudied. Through metatranscriptomic analyses of publicly available data, we uncovered a diverse range of viruses associated with the spider mite, including crop-infecting pathogenic species such as <i>Potato virus Y</i> and <i>Cherry virus A</i>, and fourteen previously unknown viruses across several families (e.g., <i>Virgaviridae</i>, <i>Dicistroviridae</i>, <i>Kitaviridae</i>, <i>Betaflexiviridae</i>, and <i>Nudiviridae</i>). Taking advantage of mite samples under different conditions, we also assessed the impact of biotic (<i>Wolbachia</i> and <i>Spiroplasma</i> infection) and abiotic stresses (pesticide exposure and temperature stress) on the <i>T. truncatus</i> virome. Interestingly, <i>Wolbachia</i> appeared to restrict viral infections in <i>T. truncatus</i> by reducing viral diversity and abundance, with a pronounced effect on dicistroviruses. Surprisingly, a similar effect also observed with <i>Spiroplasma</i>. However, the viral restriction phenotype vanishes in co-infected mites. Transcriptomics analysis of singly-infected mites revealed upregulation of piRNA and autophagy-related genes, while lipid metabolism processes-related genes were downregulated, indicating an endosymbiont-sharing mechanisms of viral interference. Although the impact of abiotic stressors on the virome was not statistically significant, <i>Potato virus Y</i> and TtDV-2 viruses were absent in abamectin-exposed mites, suggesting a potential reduction in the viral diversity, while heat-stressed mites exhibited slightly higher viral diversity compared to those raised at regular temperatures. Overall, our work provides a detailed analysis of the <i>T. truncatus</i> virome, shedding light on how endosymbionts and environmental factors shape viral dynamics and offering potential insights for pest management strategies.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1570606"},"PeriodicalIF":4.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of aflatoxin contamination in protein-rich pulses using a GFP-expressing <i>Aspergillus flavus</i> strain.","authors":"Emily H Branstad-Spates, Christine M Sickler, Matthew D Lebar, Carol Carter-Wientjes, Kanniah Rajasekaran","doi":"10.3389/fmicb.2025.1587035","DOIUrl":"10.3389/fmicb.2025.1587035","url":null,"abstract":"<p><strong>Background: </strong>Mycotoxigenic fungi pose significant threats to food safety and marketability. Crop-specific differences in susceptibility to these fungi can influence contamination levels.</p><p><strong>Objectives: </strong>The resistance or susceptibility of protein-rich pulse crops-chickpeas (<i>Cicer arietinum</i> L. cv. CDC Frontier), lentils (<i>Lens culinaris</i> Medik cv. Eston), peas (<i>Pisum sativum</i> L. cv. LeRoy), and corn (<i>Zea mays</i> L. cv. H97C) to infection by <i>Aspergillus flavus</i> were evaluated using a kernel screening assay (KSA).</p><p><strong>Methodology: </strong><i>A. flavus</i> strain 70 (AF-70) expressing green-fluorescent protein (GFP) was used to quantify fungal spread and mycotoxin production. Fungal infection and toxin levels, including aflatoxins (AFB₁, AFB₂), cyclopiazonic acid (CPA), and α-aflatrem, were monitored at 2-day intervals over a 10-day period post inoculation.</p><p><strong>Results: </strong>Although all seeds were infected by <i>A. flavus</i>, corn produced significantly higher levels of AFB₁ and AFB₂ compared to pulses. However, pulses accumulated relatively higher levels of CPA and α‑aflatrem.</p><p><strong>Conclusion: </strong>While pulses may be less susceptible to aflatoxin contamination than corn, the elevated concentrations of CPA and α‑aflatrem underscore the need for further toxicological evaluation and mechanistic studies. Future research should explore the underlying resistance mechanisms from field to storage to better ensure crop safety.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1587035"},"PeriodicalIF":4.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Gut microbiota's role in high-altitude animal adaptation.","authors":"Lin Zhang, Wei Zhu, Kashif Ur Rehman, Huan Li","doi":"10.3389/fmicb.2025.1613028","DOIUrl":"10.3389/fmicb.2025.1613028","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1613028"},"PeriodicalIF":4.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12158974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the soil layer on the soil microbial diversity and composition of <i>Pinus yunnanensis</i> at the Ailao Mountains subtropical forest.","authors":"Haonan Qiao, Qingchao Zeng, Francis Martin, Qi Wang","doi":"10.3389/fmicb.2025.1558906","DOIUrl":"10.3389/fmicb.2025.1558906","url":null,"abstract":"<p><p>Microbial communities residing in forest soils play crucial roles in decomposing organic matter and recycling nutrients, making these ecosystems one of the most diverse habitats on Earth. However, the composition and function of these complex and diverse microbiomes across different soil layers remain largely unknown. In this study, we collected soil samples from various layers and analysed the bacterial and fungal community compositions in experimental forest ecosystems using sequencing techniques. Our findings revealed that the soil layer was the primary factor influencing microbial communities, whereas sampling season had only a marginal effect. The most prevalent bacterial phyla and fungal classes were Acidobacteria, Actinobacteria, Armatimonadetes, Bacteroidetes, Firmicutes, Planctomycetes, Proteobacteria, Verrucomicrobia, and Agaricomycetes. Owing to the heterogeneity of the soil layer environment, we observed distinct patterns in the bacterial and fungal microbiomes across different layers. Moreover, the soil layer affected the network complexity, with fungi exhibiting higher complexity in the upper layer, whereas bacteria showed the opposite trend. Additionally, the dominant bacterial and fungal taxa across all soil layers belonged predominantly to Acidobacteria and Agaricomycetes, respectively. These findings underscore the significance of soil layers in shaping soil microbial communities and highlight the composition and co-occurrence patterns of the microbial communities within these layers.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1558906"},"PeriodicalIF":4.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic diversity and community structure of <i>Planctomycetota</i> from plant biomass-rich environments.","authors":"Dominika Klimek, Olga Maria Lage, Magdalena Calusinska","doi":"10.3389/fmicb.2025.1579219","DOIUrl":"10.3389/fmicb.2025.1579219","url":null,"abstract":"<p><p>Biomass-rich environments host diverse microbial communities that contribute to the degradation and recycling of organic matter. Understanding the community structure within these habitats is essential for elucidating the ecological roles and metabolic capacities of specific microbial groups. Here, we conducted an analysis of biomass-rich environments including diverse soil types, sediments, anaerobic digesters, termite guts, termite nests and other decaying biomasses, to explore the phylogenetic diversity and community structure of the <i>Planctomycetota</i> phylum, using short-read 16S rRNA gene amplicon sequencing. All sampled environments showed presence of <i>Planctomycetota</i>, with relative abundance ranging from nearly absent in animal manure to approximately 10% in soils. Across all samples, virtually 1,900 operational taxonomic units (OTUs) were identified, classified into diverse classes within <i>Planctomycetota</i>. Planctomycetotal phylogenetic diversity was highest in soils and sediments, while termite guts, exhibiting the lowest phylogenetic diversity, were dominated by a few core OTUs shared across different termite species. Notably, a single OTU, closely matching the 16S rRNA gene sequence of the <i>Singulisphaera</i> genus, was detected in all environments, though with relative abundance ranging from only a few reads to over 6% of the planctomycetotal community. Four environments such as soil, sediment, termite nest and decaying biomasses showed similar community structure with predominant genera such as <i>Tepidisphaera, Telmatocola</i>, and distantly related to <i>Thermogutta</i>, and <i>Anatilimnicola</i>. However, among these environments, weighted UniFrac analysis revealed that planctomycetotal communities in termite nests exhibited greater phylogenetic relatedness. Termite gut communities were the most divergent, followed by those in anaerobic digesters, where OTUs assigned to <i>Anaerobaca</i> and <i>Anaerohalosphaera</i> were the most abundant. Termite gut and phytoplankton bloom samples were dominated by OTUs affiliated with <i>Pirellulales</i>, suggesting their host-specific associations. Animal manure showed the presence of <i>Planctomycetota</i>, with 25% of detected OTUs not recognized by the SILVA database, possibly representing a novel, host-specific lineage distantly related to the <i>Pirellulales</i> order.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1579219"},"PeriodicalIF":4.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant developmental stage drives the assembly and functional adaptability of endophytic microbial communities.","authors":"Min Yang, Jindan Wang, Ying Qi, Penghua Gao, Lifang Li, Jianwei Guo, Yongteng Zhao, Jiani Liu, Zebin Chen, Jianrong Zhao, Lei Yu","doi":"10.3389/fmicb.2025.1492141","DOIUrl":"10.3389/fmicb.2025.1492141","url":null,"abstract":"<p><strong>Introduction: </strong>The seeds of <i>Amorphophallus muelleri</i> represent a unique category of herbaceous seeds that arise from triploid apomixis. They necessitate an exceptionally protracted maturation phase of 8 months, followed by a dormancy period of 4 months, before they can germinate and give rise to fully formed new plants. Currently, the connection between endophytic microbial communities in <i>A. muelleri</i> seeds and the host plant's development is largely unexplored.</p><p><strong>Methods: </strong>Herein, we analyzed the temporal dynamics of the endophytic bacterial and fungal communities from seed germination to seedling establishment (seven stages) through amplicon sequencing.</p><p><strong>Results and discussion: </strong>The results showed that plant developmental stage explained the large variation in endophytic bacterial and fungal communities in <i>A. muelleri</i> and that multiple microbial attributes (e.g., α, β-diversity, community composition, and bacterial and fungal ecological networks) are driven by the developmental state of <i>A. muelleri</i>. Metagenomic analyses further indicated that the four stages after rooting have higher microbial functional diversity. Microbial functional genes involved in cell wall/membrane/envelope biogenesis, inorganic ion transport and metabolism, and carbon degradation were abundant in <i>A. muelleri</i> seeds from Stage 1 to Stage 3 (before rooting). From Stage 4 to Stage 7 (after rooting), microbial functional genes involved in the carbon, nitrogen and phosphorus cycles, starch and sucrose metabolism, and energy production and conversion were more abundant. Coincidentally, more abundant Proteobacteria, and Basidiomycota taxa related to carbon degradation were found in stages 1-3, while more <i>Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium</i> and <i>Stenotrophomonas</i> taxa associated with nitrogen cycling and plant growth promotion were observed in stages 4-7. These findings have greatly improved our basic understanding of the assembly and functional adaptability of the endophytic microbiome during <i>A. muelleri</i> plant development and are helpful for the mining, development and utilization of functional microbial resources.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1492141"},"PeriodicalIF":4.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12158941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal changes in arbuscular mycorrhizal fungi communities and their driving factors in <i>Xanthoceras sorbifolium</i> plantations.","authors":"Yuexin Zhang, Yunxia Ma, Xiuzhi Ma, Cuiwei Li","doi":"10.3389/fmicb.2025.1579868","DOIUrl":"10.3389/fmicb.2025.1579868","url":null,"abstract":"<p><p>Arbuscular mycorrhizal fungi (AMF) communities are influenced by soil nutrients and plant and litter traits during forest ecosystem development. However, the extent to which these factors influence AMF communities in <i>Xanthoceras sorbifolium</i> plantations is unclear. In this study, rhizosphere soil samples were collected from 5-, 13-, 24-, 35-, 47-, and 56-year-old <i>X. sorbifolium</i> plantations. The AMF community was analyzed using Illumina MiSeq sequencing, and AMF spores were isolated and identified by wet sieving. The results showed that <i>X. sorbifolium</i> can establish a symbiotic relationship with AMF at different forest ages. In total, 5,876 AMF amplicon sequence variant (ASVs) were obtained from the soil samples and classified into 1 phylum, 4 classes, 6 orders, 12 families, and 15 genera. <i>Glomus</i> was the dominant genus. In addition, the diversity of AMF communities increased and then decreased with the age of <i>X. sorbifolium</i>, with no significant changes observed between 35-, 47-, and 56-year-old plantations. AMF community variance was primarily determined by soil-specific factors, with soil pH and root C content being the most influential. The results revealed the factors that drive AMF communities during the development of <i>X. sorbifolium</i> and provide valuable information for future conservation and planting management.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1579868"},"PeriodicalIF":4.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12158962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}