Frontiers in Microbiology最新文献

{"title":"<i>Lactiplantibacillus plantarum</i> improves the growth performance and meat quality of broilers by regulating the cecal microbiota and metabolites.","authors":"Yu Liu, Zhisheng Wang, Wenshuo Xi, Junmeng Yuan, Kai Zhang, Huawei Liu, Jinshan Zhao, Yang Wang","doi":"10.3389/fmicb.2025.1519552","DOIUrl":"10.3389/fmicb.2025.1519552","url":null,"abstract":"<p><p>Gut microbiota can digest and ferment feed into metabolites to influence the meat quality. Probiotics are used to regulate the gut microbiota. In this study, a total of 360 broilers were assigned to 4 treatments (10 broilers per cage): control (Con), low dose of <i>Lactiplantibacillus plantarum</i> HW1 (Lp_L), medium dose of Lp (Lp_M) and high dose of Lp (Lp_H) for a 42-day experimental period. Results showed that the Lp treatments improved the growth performance, carcass traits, breast meat quality, and also influenced the fatty acids composition, including the decrease of n-6PUFA/n-3PUFA, and the increase of C18:3n3, ∑n-3PUFA and PUFA/SFA. The lipid metabolism-related gene expressions in the liver showed that Lp treatments increased the expression of <i>AMPK</i>, <i>CPT-1α</i>, <i>PPARα</i>, <i>ATGL</i> and also decreased the expression of <i>PPARγ</i>, <i>SREBP-1c</i>, <i>ACC</i>, <i>FAS</i>, <i>LPL</i>, and <i>SCD</i>. Moreover, the abundances of gut microbiota, such as Synergistaceae and <i>Synergistes</i> were influenced by the Lp treatments. Functional prediction of the gut microbiota indicated that pathways, including pancreatic secretion and spliceosome were enriched by the Lp treatments. Untargeted metabolomics revealed that the Lp treatments altered the content of metabolites, such as 6-ketomyristic acid and indole-3-acetamide. These metabolites were enriched in pathways including fatty acid metabolism. Correlation analyses revealed potential interactions between growth performance and meat quality, as well as gut microbiota (<i>Synergistes</i>, etc.) and metabolites (6-ketomyristic acid, etc.). Overall, our data show that the Lp treatments significantly improved the growth performance, carcass traits and meat quality of broilers by regulating fatty acids, gut microbiota and metabolites.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1519552"},"PeriodicalIF":4.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chikungunya virus infection in the skin: histopathology and cutaneous immunological response.","authors":"Natália Gedeão Salomão, Luciana Araújo, Luiz José de Souza, Anna Luiza Young, Carlos Basílio-de-Oliveira, Rodrigo Panno Basílio-de-Oliveira, Jorge José de Carvalho, Priscilla Conrado Guerra Nunes, Juliana Fernandes da Silva Amorim, Douglas Valiati Dos Santos Barbosa, Marciano Viana Paes, Kíssila Rabelo, Flavia Dos Santos","doi":"10.3389/fmicb.2025.1497354","DOIUrl":"10.3389/fmicb.2025.1497354","url":null,"abstract":"<p><p><i>Alphavirus chikungunya</i> virus (CHIKV) is an arbovirus, belonging to the <i>Togaviridae</i> family. The disease caused by CHIKV generally evolves with spontaneous resolution in a few weeks; however, progression to a chronic disease may occur. The most common symptoms are fever, myalgia, and arthralgia; however, skin manifestations may occur in 40 to 80% of infected individuals. Morbilliform and maculopapular erythematous eruptions, vesiculobullous lesions, generalized erythema, maculopapular eruption and skin peeling, hypermelanosis, painful oral lesions, and urticarial lesions have been reported. Usually, these manifestations disappear, but they can become sequelae. Since the skin is the first line of defense against CHIKV infection, in this study, we aimed to investigate the immunohistopathological aspects of the skin of infected individuals during the acute phase of the disease by performing histopathological and ultrastructural analysis, detection and quantification of the viral genome, detection of viral antigen and immune cells, and cytokines/chemokines' characterization. The main histopathological findings were perivascular and inflammatory infiltrates, blood capillary ectasia, and interstitial edema. The immunohistochemistry revealed CHIKV antigen in the epidermis, endothelial cells, fibroblasts, and macrophages in the reticular and papillary dermis; inflammatory cells infiltrate; arrector pili muscle; sweat and sebaceous glands; and hair follicle. Moreover, inflammatory infiltrates were composed of lymphocytes (CD4⁺ and CD8⁺) and macrophages (CD68⁺) in the dermis and perivascular infiltrate. TNF-<i>α</i>, IL-6, RANTES, and VEGFR2 were expressed in the epidermis, blood vessels, sweat glands, and migrating cells. Loss of contact among adjacent keratinocytes, epidermis presenting necrotic cells, and fibroblasts with dilated cisternae in the endoplasmic reticulum and mitochondria with few cristae was observed by transmission electron microscopy. Studies involving skin immunopathogenesis during CHIKV infection are still scarce; therefore, the findings presented here can contribute to a better understanding of the disease immunopathogenesis.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1497354"},"PeriodicalIF":4.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: A long-awaited taxogenomic investigation of the family <i>Halomonadaceae</i>.","authors":"Rafael R de la Haba, David R Arahal, Cristina Sánchez-Porro, Maria Chuvochina, Stijn Wittouck, Philip Hugenholtz, Antonio Ventosa","doi":"10.3389/fmicb.2025.1549745","DOIUrl":"10.3389/fmicb.2025.1549745","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fmicb.2023.1293707.].</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1549745"},"PeriodicalIF":4.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11810961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geographic variation in abundance and diversity of <i>Acinetobacter baumannii Vieuvirus</i> bacteriophages.","authors":"Dafne Arellano-Maciel, Juan Manuel Hurtado-Ramírez, Laura Carolina Camelo-Valera, Santiago Castillo-Ramírez, Alejandro Reyes, Gamaliel López-Leal","doi":"10.3389/fmicb.2025.1522711","DOIUrl":"10.3389/fmicb.2025.1522711","url":null,"abstract":"<p><strong>Introduction: </strong>Prophages play a crucial role in the genomic diversity of <i>Acinetobacter baumannii</i>, contributing to its pathogenicity and adaptation.</p><p><strong>Methods: </strong>In this study, we induced and sequenced seven prophages from five isolates of <i>A. baumannii</i>. These were analyzed with 967 prophages identified from various isolates worldwide, plus 21 genomes of other phages infecting <i>A. baumannii</i> previously reported in NCBI. To have an overview of the populations of the prophages infecting <i>A. baumannii</i>.</p><p><strong>Results: </strong>Our analysis revealed 13 major prophage clusters within the analyzed <i>A. baumannii</i> isolates. Notably, prophages belonging to the <i>Vieuvirus</i> genus were the most prevalent. Specifically, <i>Vieuvirus</i>-related phages were frequently identified in isolates from Thailand, Mexico, China, and South Korea, which show the geographic prevalence of <i>A. baumannii</i> prophages.</p><p><strong>Discussion: </strong>This study highlights the importance of considering geographic factors to fully understand prophage diversity and their significant role in the evolutionary dynamics of <i>A. baumannii</i>.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1522711"},"PeriodicalIF":4.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Streptococcus suis</i>-associated neonatal meningitis and sepsis: characterization, antimicrobial resistance, and public health implications.","authors":"Giovanna Fusco, Rubina Paradiso, Lorena Cardillo, Maria Antonia Salvia, Saveria Dodaro, Veronica Del Monaco, Gianfranco Scarpelli, Francesca Greco, Antonio Rinaldi, Lorella Barca, Stefania Ambrogio, Antonio Limone, Esterina De Carlo, Giorgia Borriello","doi":"10.3389/fmicb.2025.1519247","DOIUrl":"10.3389/fmicb.2025.1519247","url":null,"abstract":"<p><p>Neonatal meningitis and sepsis were diagnosed in a 32-day-old preterm-born infant. <i>Streptococcus (S.) suis</i> was isolated from cerebrospinal fluid and blood. Next-generation sequencing revealed that the strain was serotype 2 sequence type 1, and contained the <i>ermB</i> and <i>tet(W)</i> genes, which are responsible for resistance to macrolides and tetracycline, along with several pilus-associated genes and 20 virulence factors. High homology was observed with previously identified human and swine strains in the same area. <i>S. suis</i> meningitis and sepsis are mainly reported in adults, related to direct contact with pigs or contaminated pork meat consumption, while it is rarely reported in children. Herein, we describe the first case of <i>S. suis</i> in a newborn associated with meningitis and antimicrobial resistance. The rates of resistance to tetracyclines, lincosamides, and macrolides for this bacterium are increasing and are creating concern worldwide. Altogether, our findings highlight the importance of investigating <i>S. suis</i> in cases of neonatal meningitis, as well as the necessity of assessing the antimicrobial profile to obtain useful information for developing targeted therapies.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1519247"},"PeriodicalIF":4.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prevalence and antimicrobial resistance of respiratory pathogens isolated from feedlot cattle in Canada.","authors":"Porjai Rattanapanadda, Dana Ramsay, Alyssa Butters, Calvin W Booker, Sherry J Hannon, Steve Hendrick, Joyce Van Donkersgoed, Brian N Warr, Sheryl P Gow, Paul S Morley","doi":"10.3389/fmicb.2025.1497402","DOIUrl":"10.3389/fmicb.2025.1497402","url":null,"abstract":"<p><strong>Objectives: </strong>The purpose of this study was to characterize the prevalence of antimicrobial resistance in <i>Mannheimia haemolytica</i>, <i>Pasteurella multocida</i>, and <i>Histophilus somni</i> isolated from healthy feedlot cattle over 2 years, and investigate factors potentially associated with recovery of resistant isolates.</p><p><strong>Methods: </strong>Deep-guarded nasopharyngeal (NP) swabs were used to sample feedlot cattle in multiple randomly selected feedlots (2019 <i>n</i> = 21, 2020 <i>n</i> = 26) at 2 timepoints. NP swabs were collected from 16 animals in each enrolled group upon entry processing and later in the feeding period. Cattle from the same groups (not necessarily the same animals) were sampled at both timepoints. Susceptibility testing was performed using the broth microdilution.</p><p><strong>Results: </strong>A total of 1,392 cattle within 47 housing groups were sampled over 2 years, providing 625 bacterial isolates for investigation. <i>Pasteurella multocida</i> (27.4%) was the most frequently isolated BRD organism, followed by <i>H. somni</i> (9%) and <i>M. haemolytica</i> (8.5%). Resistance to ≥3 antimicrobial classes was detected in 2.4% of <i>M. haemolytica</i>, 3.4% of <i>H. somni</i>, and 21.3% of <i>P. multocida</i> isolates. Potential associations were investigated between recovery of resistant organisms and time of year at sampling (quarter), sampling timepoint (arrival or second sample), days on feed (DOF) at sampling, animal age categories, and BRD risk categories. There was a significant (<i>p <</i> 0.05) increase in resistance prevalence after arrival for macrolide drugs in <i>M. haemolytica</i>, and for ampicillin, danofloxacin, enrofloxacin, spectinomycin, gamithromycin, tildipirosin, tulathromycin and tetracycline in <i>P. multocida</i> isolates. Resistance was higher in calves than in yearlings for tulathromycin in <i>H. somni</i>, and for gamithromycin, spectinomycin, tulathromycin, tildipirosin, and tetracycline for <i>P. multocida</i> (<i>p</i> < 0.05) Resistance to tetracycline, tildipirosin, and tulathromycin decreased between 61-80 DOF and 81-100 DOF when compared to 20-40 DOF, whereas for spectinomycin, resistance was lower in cattle sampled between 61-80 DOF than those sampled at 20-40 DOF for <i>P. multocida</i>.</p><p><strong>Discussion: </strong>The diversity of AMR profiles and associated risk factors between the BRD pathogens studied, underscores the importance of including all three organisms in future AMR studies in beef cattle.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1497402"},"PeriodicalIF":4.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11810952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-fat diet promotes gestational diabetes mellitus through modulating gut microbiota and bile acid metabolism.","authors":"Lei Yao, Xuefei Zhou, Xianqi Jiang, Hao Chen, Yuanliang Li, Xiao Xiong, Yan Tang, Haogang Zhang, Pengfei Qiao","doi":"10.3389/fmicb.2024.1480446","DOIUrl":"10.3389/fmicb.2024.1480446","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Gestational diabetes mellitus (GDM) is a condition characterized by glucose intolerance during pregnancy, estimated to affect approximately 20% of the whole pregnancies and is increasing in prevalence globally. However, there is still a big gap in knowledge about the association between gut microbiota associated metabolism alterations and GDM development.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;All the participants accomplished the validated internet-based dietary questionnaire for Chinese and serum, fecal samples were collected. HFD, control diet or colesevelam intervention was fed to GDM mice models or Fxr-/- mice models, with or without antibiotics cocktail treatment. Fecal microbiota transplantation were used for further validation. Gut microbiota and metabolites were detected by metagenomic sequencing and high-performance liquid chromatography-mass spectrometry, respectively. Bile acids of serum, fecal samples from human and mice were analysised. Body weight, average feed intake, blood glucose, insulin levels and oral glucose tolerance test was performed among each groups. Expression levels of Fxr, Shp and Fgf15 mRNA and protein were detected by quantitative reverse transcription polymerase chain reaction and western blot, respectively.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Our data indicated that high fat diet (HFD) was linked with higher prevalence of GDM, and HFD was positively associated with poor prognosis in GDM patients. Moreover, compared with normal diet (ND) group, GDM patients from HFD group performed a loss of gut microbiota diversity and enrichment of &lt;i&gt;Alistipes onderdonkii&lt;/i&gt;, &lt;i&gt;Lachnospiraceae bacterium 1_7_58FAA&lt;/i&gt;, and &lt;i&gt;Clostridium aspaaragiforme&lt;/i&gt; while ruduction of &lt;i&gt;Akkermansiaceae, Paraprevotell xylaniphila, and Prevotella copri&lt;/i&gt;. Additionally, HFD aggravated GDM in mice and gut microbiota depletion by antibiotics crippled the effect of excess fat intake. BAs profile altered in HFD GDM patients and mice models. Fecal microbiota transplantation (FMT) further confirmed that gut microbiota contributed to bile acids (BAs) metabolic dysfunction during HFD-associated GDM development. Mechanically, HFD-FMT administration activated &lt;i&gt;Fxr, Shp,&lt;/i&gt; and &lt;i&gt;Fgf15&lt;/i&gt; activity, disturbed the glucose metabolism and aggravated insulin resistance but not in HFD-FMT &lt;i&gt;Fxr-/-&lt;/i&gt; mice and ND-FMT &lt;i&gt;Fxr-/-&lt;/i&gt; mice. Furthermore, colesevelam intervention alleviated HFD-associated GDM development, improved BAs metabolism, suppressed &lt;i&gt;Fxr, Shp,&lt;/i&gt; and &lt;i&gt;Fgf15&lt;/i&gt; activity only in &lt;i&gt;WT&lt;/i&gt; mice but not in the &lt;i&gt;Fxr-/-&lt;/i&gt; HFD + Colesevelam group and &lt;i&gt;Fxr-/-&lt;/i&gt; HFD group. HFD induced GDM and contributed to poor prognosis in GDM parturients through inducing gut microbial dysbiosis and metabolic alteration, especially appeared in BAs profile. Moreover, Fxr pathway participated in regulating HFD-associated gut microbiota disordered BAs metabolites and aggravating GDM in mice.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Discussion: &lt;","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1480446"},"PeriodicalIF":4.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11810896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the microecological mechanisms of phosphate-solubilizing <i>Pseudomonas asiatica</i> JP233 through metagenomics: insights into the roles of rhizosphere microbiota and predatory bacteria.","authors":"Yuhan Tang, Linlin Wang, Jing Fu, Fangyuan Zhou, Hailei Wei, Xiaoqing Wu, Susu Fan, Xinjian Zhang","doi":"10.3389/fmicb.2025.1538117","DOIUrl":"10.3389/fmicb.2025.1538117","url":null,"abstract":"<p><p>The effects of phosphate-solubilizing bacteria (PSB) on plant productivity are high variable under field conditions. Soil phosphorus (P) levels are proposed to impact PSB performance. Furthermore, the effect of exogenous PSB on rhizosphere microbial community and their functions are largely unexplored. Our study examined how different P background and fertilization affected the performance of PSB <i>Pseudomonas asiatica</i> JP233. We further conducted metagenomic sequencing to assess its impact on rhizosphere microbiota and functions, with a focus on genes related to soil P cycling. We found that JP233 could enhance P solubilization and tomato growth to different extent in both high and low P soils, irrespective of P fertilization. It was particularly effective in high P soil without extra fertilization. JP233 altered the rhizosphere microbial community, boosting taxa known for plant growth promotion. It also changed soil gene profiling, enriching pathways related to secondary metabolite biosynthesis, amino acids, carbon metabolism, and other key processes. Particularly, JP233 increased the abundance of most P cycle genes and strengthened their interconnections. Populations of certain predatory bacteria increased after JP233 inoculation. Our findings provide valuable insights into PSB's mechanisms for P solubilization and plant growth promotion, as well as potential adverse impacts of resident microbes on bioinoculants.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1538117"},"PeriodicalIF":4.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11810911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of the <i>Streptococcus pneumoniae</i> competence development <i>in vitro</i> versus <i>in vivo</i> during pneumonia-derived sepsis.","authors":"Sook Yin Chong, Shi Qian Lew, Tauqeer Alam, Christopher A Gaulke, Gee W Lau","doi":"10.3389/fmicb.2025.1540511","DOIUrl":"10.3389/fmicb.2025.1540511","url":null,"abstract":"<p><strong>Introduction: </strong>The <i>Streptococcus pneumoniae</i> (pneumococcus) competence regulon is well-known for regulating genetic transformation but is also important for virulence. Some pneumococcal strains can enter a transient competent state for genetic transformation in an optimized competence-inducing medium when the threshold level of the peptide pheromone competence stimulating peptide is attained; upregulating the expression of three distinct phases of \"early\", \"late\" and \"delayed\" competence genes. Recently, we discovered that pneumococcus can naturally enter a prolonged competent state during acute pneumonia in mice. However, mechanisms driving competence development during host infection are rarely examined, and a direct comparison between <i>in vitro</i> and <i>in vivo</i> competence induction has not been performed.</p><p><strong>Methods: </strong>We conducted a comparative gene expression analysis of pneumococcal competence development <i>in vitro</i> versus <i>in vivo</i> during pneumonia-derived sepsis in mice. We examined existing RNA-Seq data and performed validation using RNA obtained from an independent replicate experiment.</p><p><strong>Results and discussion: </strong>Our analysis revealed both similarities and differences in the expression of \"early\", \"late\", and \"delayed\" competence between <i>in vitro</i> versus during pneumonia-derived sepsis. Our results may reveal new aspects of pneumococcal competence biology.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1540511"},"PeriodicalIF":4.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut dysbiosis in primary sarcopenia: potential mechanisms and implications for novel microbiome-based therapeutic strategies.","authors":"Wei Yang, Si-Cong Si, Wei-Hua Wang, Jing Li, Yi-Xin Ma, Huan Zhao, Jia Liu","doi":"10.3389/fmicb.2025.1526764","DOIUrl":"10.3389/fmicb.2025.1526764","url":null,"abstract":"<p><p>Primary sarcopenia is characterized by a progressive loss of skeletal muscle mass, strength, and physical function that occurs with aging. Despite the related adverse or even serious health outcomes, no medications are currently available for treating primary sarcopenia. Here, we discuss recent advancements in understanding the mechanistic role of gut microbiota-muscle cross-talk in primary sarcopenia, and the therapeutic implications. The mechanistic insights encompass a causal role of gut dysbiosis in primary sarcopenia, potentially mediated through gut microbiota-derived bioactive metabolites, such as short-chain fatty acids (SCFAs), secondary bile acids, and their associated signaling pathways, which may be translated into the development of new microbiome-based treatment and diagnostic approaches. Furthermore, we identify challenges that need addressing in future studies to facilitate the translation into potential novel treatment and differential diagnosis for older individuals with sarcopenia.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1526764"},"PeriodicalIF":4.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11810907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}