Frontiers in Microbiology最新文献

{"title":"Functional analysis of the <i>Escherichia coli mrdA</i> gene in melittin resistance.","authors":"Chong-Yi Zhao, Xiao Li, Ting Zhao, Ying Liu, Xue-Shan Xia, Xiao-Mei Wu","doi":"10.3389/fmicb.2024.1516808","DOIUrl":"10.3389/fmicb.2024.1516808","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study is to examine the functional role and resistance mechanisms of the <i>Escherichia coli</i> (<i>E. coli</i>) peptidoglycan transpeptidase gene, <i>mrdA</i>, in resistance to melittin.</p><p><strong>Methods: </strong>The resistance of <i>E. coli</i> strains with either knockout or overexpression of the <i>mrdA</i> gene to melittin was initially assessed. The differences in melittin absorption between these two strains were evaluated following depletion and heterologous expression of the <i>mrdA</i> gene. Subsequently, peptidoglycan was extracted from the strains to determine its capacity to adsorb melittin. Finally, the morphological changes in different strains induced by melittin exposure were examined under scanning electron microscopy. These analyses served to validate the role of peptidoglycan transpeptidase <i>mrdA</i> in melittin resistance and to hypothesize its potential resistance mechanism.</p><p><strong>Results: </strong>The results clearly indicated a direct correlation between the degree of peptidoglycan cross-linking in <i>E. coli</i> and its enhanced resistance to melittin. Specifically, we found that increased cross-linking of peptidoglycan led to a thickening of the bacterial cell wall and a reduction in pore size. These structural changes potentially decrease the damage to the cell wall caused by melittin, as the thicker cell wall and smaller pores reduce the ability of melittin to penetrate and access the interior of bacterial cells. This mechanism effectively limits the contact between melittin and bacterial components, minimizing its destructive effects, and thereby conferring resistance to melittin in the bacteria.</p><p><strong>Conclusion: </strong>This study is the first to elucidate the role of peptidoglycan in the cell wall of <i>E. coli</i> in the context of antimicrobial peptide resistance. Novel ideas have been proposed for the development of antibacterial drugs targeting the peptidoglycan of Gram-negative bacteria.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1516808"},"PeriodicalIF":4.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPV integration profiling using nanopore sequencing and association with cervical precancerous lesion.","authors":"Ying Hou, Shoufeng Ni, Xin Liu, Xingyu Liu, Nan Wang, Fuqiang Xu, Jianyong Gao, Yanmei Li, Yuxiang Zhou, Huadong Tang, Meina Bian, Xiulan Li, Lili Zhang, Weiwei Wang, Qing Liu","doi":"10.3389/fmicb.2025.1522550","DOIUrl":"10.3389/fmicb.2025.1522550","url":null,"abstract":"<p><strong>Objectives: </strong>HPV infection and HPV DNA integration can lead to cervical cancer, but the relationship with lesion severity is unclear. This study aimed to investigate the correlation between HPV integration profile and cervical lesion extent.</p><p><strong>Materials and methods: </strong>Twenty patients representing cervicitis, CIN I, CIN II, and CIN III underwent nanopore sequencing for HPV genotype and integration site analysis. HPV integration profiles were correlated with lesion severity. Gene Ontology (GO) and KEGG analysis were used to identify stage-specific genes and pathways.</p><p><strong>Results: </strong>HPV integration rates were 60, 60, 100, and 100% for cervicitis, CIN I, CIN II, and CIN III, respectively, with varying numbers of integrated genes. Each group had specific stage-related genes, with 83 shared genes linked to neuron development and cell-cell processes. CIN II and CIN III displayed more cancer-related pathway enrichment than earlier stages.</p><p><strong>Conclusion: </strong>A positive correlation exists between HPV integration frequency and cervical lesion stage. Late-stage lesions showed heightened enrichment in cancer-related pathways through specific HPV-integrated genes.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1522550"},"PeriodicalIF":4.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of compound polysaccharide on immunity, antioxidant capacity, gut microbiota, and serum metabolome in kittens.","authors":"Yixuan Xie, Shiyan Jian, Limeng Zhang, Baichuan Deng","doi":"10.3389/fmicb.2025.1500961","DOIUrl":"10.3389/fmicb.2025.1500961","url":null,"abstract":"<p><strong>Introduction: </strong>This study was conducted to investigate the effects of compound polysaccharides (CP), composed of <i>Astragalus</i> polysaccharide and <i>Poria cocos</i> polysaccharide, on immunity, antioxidant capacity, gut microbiota, and serum metabolome in kittens.</p><p><strong>Methods: </strong>A total of 14 4-month-old kittens, with an average body weight of 2.39 kg, were used in a 56-day experiment. They were randomly assigned to the control (CON) group (<i>n</i> = 7) and CP group (<i>n</i> = 7). Blood samples and fresh feces were collected at the end of the experimental period.</p><p><strong>Results: </strong>The results displayed that supplementation with CP increased the concentrations of serum immunoglobulin A, immunoglobulin G, interleukin 6, and tumor necrosis factor-<i>α</i> (<i>p <</i> 0.05). However, there was no difference in the concentrations of serum amyloid A between the two groups (<i>p</i> > 0.05). Furthermore, the serum biochemical parameters of all the kittens were within the reference range. The relative abundance of beneficial bacteria (<i>norank_f__Butyricicoccaceae</i> and <i>Bacteroides plebeius</i>) was higher in the CP group (<i>p</i> < 0.05), while the opportunistic pathogen (<i>Anaerotruncus</i>) was lower in the CP group (<i>p</i> < 0.05). In addition, serum metabolomic analysis demonstrated that the differential metabolites, including arachidonic acid, dihomo-gamma-linolenic acid, and glycine, and the relevant metabolic pathway, including glyoxylate and dicarboxylate metabolism, glycine, serine, and threonine metabolism, and biosynthesis of unsaturated fatty acids, were implicated in regulating immune function in the kitten after CP treatment.</p><p><strong>Conclusion: </strong>CP supplementation can enhance immune function in kittens and increase the relative abundance of beneficial gut microbiota, and does not lead to generalized inflammation. Dietary supplementation with CP may generate nutritional benefits in kittens, and this study offers insight into the development of functional pet food for kittens.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1500961"},"PeriodicalIF":4.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of linear B-cell epitopes of Senecavirus A VP2 protein using monoclonal antibodies.","authors":"Yao Jiang, Zhenhua Guo, Maoyang Weng, Linlin Chen, Qingmei Li, Lei Zhang, Songlin Qiao, Gaiping Zhang","doi":"10.3389/fmicb.2025.1546925","DOIUrl":"10.3389/fmicb.2025.1546925","url":null,"abstract":"<p><strong>Introduction: </strong>Senecavirus A (SVA) is an emerging vesicular pathogen in swine with clinical signs similar to those of foot-and-mouth disease, porcine vesicular disease and vesicular stomatitis, making the control of swine vesicular disease outbreaks difficult. Therefore, the development of effective diagnostics and vaccines for SVA has become critical. VP2 is a structural protein that elicits a strong immune response, which positions it a candidate for diagnostic and vaccine development.</p><p><strong>Methods: </strong>In this study, five high-titer monoclonal antibodies (mAbs) were produced using hybridoma technology. Twenty-eight peptides covering the entire VP2 sequence were synthesised by overlapping peptide synthesis, and the positive peptides were screened with the five mAbs by ELISA and Dot-blotting. The peptides were then further truncated to identify the minimal epitope regions based on immunoinformatics analyses.</p><p><strong>Results: </strong>Four mAbs were identified that reacted with peptide 15 and one mAb reacted with peptide 26. Further truncation of these peptides led to the identification of two novel minimal epitopes: 156-NEEQWV-161 and 262-VRPTSPYFN-270. Structural and sequence alignment analyses revealed that epitope 156-NEEQWV-161 is located in the flex-loop region of the VP2, whereas epitope 262-VRPTSPYFN-270 is located in the β-sheet of the VP2. Both epitopes were highly conserved among typical SVA isolates from different countries.</p><p><strong>Discussion: </strong>This study identifies two novel B-cell epitopes on the VP2, contributing to the development of VP2-based diagnostic tools with clinical applications. The findings also provide valuable material for the design of novel vaccines against SVA, offering new insights into the immune response to this pathogen.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1546925"},"PeriodicalIF":4.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating sequencing methods with machine learning for antimicrobial susceptibility testing in pediatric infections: current advances and future insights.","authors":"Zhuan Zou, Fajuan Tang, Lina Qiao, Sisi Wang, Haiyang Zhang","doi":"10.3389/fmicb.2025.1528696","DOIUrl":"10.3389/fmicb.2025.1528696","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) presents a critical challenge in clinical settings, particularly among pediatric patients with life-threatening conditions such as sepsis, meningitis, and neonatal infections. The increasing prevalence of multi- and pan-resistant pathogens is strongly associated with adverse clinical outcomes. Recent technological advances in sequencing methods, including metagenomic next-generation sequencing (mNGS), Oxford Nanopore Technologies (ONT), and targeted sequencing (TS), have significantly enhanced the detection of both pathogens and their associated resistance genes. However, discrepancies between resistance gene detection and antimicrobial susceptibility testing (AST) often hinder the direct clinical application of sequencing results. These inconsistencies may arise from factors such as genetic mutations or variants in resistance genes, differences in the phenotypic expression of resistance, and the influence of environmental conditions on resistance levels, which can lead to variations in the observed resistance patterns. Machine learning (ML) provides a promising solution by integrating large-scale resistance data with sequencing outcomes, enabling more accurate predictions of pathogen drug susceptibility. This review explores the application of sequencing technologies and ML in the context of pediatric infections, with a focus on their potential to track the evolution of resistance genes and predict antibiotic susceptibility. The goal of this review is to promote the incorporation of ML-based predictions into clinical practice, thereby improving the management of AMR in pediatric populations.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1528696"},"PeriodicalIF":4.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive epidemiological profiling of poultry-derived <i>Salmonella</i> spp. in Shandong, China, 2019-2022: a longitudinal study of prevalence, antibiotic resistances, virulence factors and molecular characteristics.","authors":"Lele Chen, Yuxia Shi, Minge Wang, Yubao Li, Zhenshu Si","doi":"10.3389/fmicb.2025.1541084","DOIUrl":"10.3389/fmicb.2025.1541084","url":null,"abstract":"<p><p><i>Salmonella</i> spp., as a major foodborne pathogen, pose significant threats to public health globally and has been an important zoonotic contamination for poultry industry that should receive increasing attentions. This study aimed to comprehensively investigate the prevalence, antimicrobial resistances, virulence factors, and plasmid types of <i>Salmonella</i> isolates collected from chickens, ducks, and geese across eight cities in Shandong between 2019 and 2022. Out of 300 samples, 53 <i>Salmonella</i> strains (17.67%) were isolated, with varied prevalence from 8.33% to 25.00% in different cities of Shandong. A total of seven serotypes were identified among the 53 <i>Salmonella</i> isolates, wherein the <i>S.</i> Enteritidis (45.28%), <i>S.</i> Pullorum (22.64%) and <i>S.</i> Typhimurium (16.98%) were identified as the most prevalent. Whole-genome sequencing analysis revealed that ST11, ST92, and ST19 were the predominant sequence types for <i>S.</i> Enteritidis, <i>S.</i> Pullorum, and <i>S.</i> Typhimurium, respectively. Phylogenetic analysis indicated that potential clonal spread of <i>S.</i> Enteritidis, <i>S.</i> Pullorum, and <i>S.</i> Typhimurium occurred across different regions, particularly the evidences supported that the <i>S.</i> Typhimurium isolates were dispersed in a cross-species manner. Finally, the phenotypic and genotypic profiling of antibiotic resistance among the isolates revealed that these isolates were multidrug resistant with corresponding antibiotic resistance genes (ARGs) including <i>bla</i> _TEM, <i>aac</i>, <i>aph</i>, <i>tet</i>(A), and <i>tet</i>(B) to confer them with resistances to commonly-used veterinary drugs such as <i>β</i>-lactams, quinolones, macrolides. To sum, this study provides valuable insights into the current epidemiology of <i>Salmonella</i> in poultry industry in one of the biggest provinces in China, and shedding the light on the urgent necessity for further approaches to prevent and decontaminate such MDR <i>Salmonella</i> in livestock under One Health concept.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1541084"},"PeriodicalIF":4.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing surfactin yield in <i>Bacillus velezensis</i> BN to enhance biocontrol efficacy and rhizosphere colonization.","authors":"Tongshu Liu, Yanli Zheng, Litao Wang, Xu Wang, Haiyan Wang, Yongqiang Tian","doi":"10.3389/fmicb.2025.1551436","DOIUrl":"10.3389/fmicb.2025.1551436","url":null,"abstract":"<p><strong>Introduction: </strong>Surfactins, a class of lipopeptide biosurfactants secreted by plant growth-promoting rhizobacteria (PGPR), have garnered significant attention due to their dual functionality in promoting plant growth and controlling plant diseases. Their potential as biopesticides is underscored by their unique physicochemical properties and biological activities. However, the practical application of surfactin is currently limited by its low yield in natural strains.</p><p><strong>Methods: </strong>This study aimed to optimize the culture conditions for <i>Bacillus velezensis</i> BN, a strain with exceptional biocontrol properties, to enhance its surfactin yield. Critical factors, including nitrogen sources and amino acid supplementation, were systematically investigated to determine their impact on surfactin production.</p><p><strong>Results: </strong>The study revealed that nitrogen sources and amino acid supplementation were pivotal factors influencing surfactin yield. Compared to the baseline, these factors resulted in a remarkable 5.94-fold increase in surfactin production. Furthermore, a positive correlation was established between surfactin yield and biocontrol efficacy. Enhanced surfactin yield was associated with improved antifungal activity, biofilm formation, and rhizosphere colonization capacity of <i>B. velezensis</i> BN on potato plantlets.</p><p><strong>Discussion: </strong>These findings provide novel insights into the practical application of surfactin and establish a scientific foundation for the development of innovative and eco-friendly antifungal agents suitable for agricultural use. The results demonstrate that optimizing culture conditions can significantly enhance surfactin yield and biocontrol efficacy, thereby highlighting the potential for sustainable agricultural practices.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1551436"},"PeriodicalIF":4.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of aflatoxin contamination outbreaks in Texas corn using mechanistic and machine learning models.","authors":"Lina Castano-Duque, Angela Avila, Brian M Mack, H Edwin Winzeler, Joshua M Blackstock, Matthew D Lebar, Geromy G Moore, Phillip Ray Owens, Hillary L Mehl, Jianzhong Su, James Lindsay, Kanniah Rajasekaran","doi":"10.3389/fmicb.2025.1528997","DOIUrl":"10.3389/fmicb.2025.1528997","url":null,"abstract":"<p><p>Aflatoxins are carcinogenic and mutagenic mycotoxins that contaminate food and feed. The objective of our research is to predict aflatoxin outbreaks in Texas-grown maize using dynamic geospatial data from remote sensing satellites, soil properties data, and meteorological data by an ensemble of models. We developed three model pipelines: two included mechanistic models that use weekly aflatoxin risk indexes (ARIs) as inputs, and one included a weather-centric model; all three models incorporated soil properties as inputs. For the mechanistic-dependent models, ARIs were weighted based on a maize phenological model that used satellite-acquired normalized difference vegetation index (NDVI) data to predict maize planting dates for each growing season on a county basis. For aflatoxin outbreak predictions, we trained, tested and validated gradient boosting and neural network models using inputs of ARIs or weather, soil properties, and county geodynamic latitude and longitude references. Our findings indicated that between the two ARI-mechanistic models evaluated (AFLA-MAIZE or Ratkowsky), the best performing was the Ratkowsky-ARI neural network (nnet) model, with an accuracy of 73%, sensitivity of 71% and specificity of 74%. Texas has significant geographical variability in ARI and ARI-hotspot responses due to the diversity of agroecological zones (hot-dry, hot-humid, mixed-dry and mixed-humid) that result in a wide variation of maize growth and development. Our Ratkowsky-ARI nnet model identified a positive correlation between aflatoxin outbreaks and prevalence of ARI hot-spots in the hot-humid areas of Texas. In these areas, temperature, precipitation and relative humidity in March and October were positively correlated with high aflatoxin contamination events. We found a positive correlation between aflatoxin outbreaks and soil pH in hot-dry and hot-humid regions and minimum saturated hydraulic conductivity in mixed-dry regions. Conversely, there was a negative relationship between aflatoxin outbreaks and maximum soil organic matter (hot-dry region), and calcium carbonate (hot-dry, and mixed-dry). It is likely soil fungal communities are more diverse, and plants are healthier in soils with high organic matter content, thereby reducing the risk of aflatoxin outbreaks. Our results demonstrate that intricate relationships between soil hydrological parameters, fungal communities and plant health should be carefully considered by Texas corn growers for aflatoxin mitigation strategies.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1528997"},"PeriodicalIF":4.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of the microbial composition of three packaged sliced dry-cured hams from a Chinese market.","authors":"Xin Luo, Ying Shen","doi":"10.3389/fmicb.2025.1531005","DOIUrl":"10.3389/fmicb.2025.1531005","url":null,"abstract":"<p><p>Ham, a widely consumed and culturally significant food, undergoes fermentation and aging processes that contribute to its distinctive flavor and texture. These processes are influenced by a complex interplay of microbial communities, which vary by the production region. Understanding these microbial dynamics can provide insights into flavor development and quality improvements in ham. In this study, the microbial communities found in ham produced in three distinct regions were compared, revealing that bacteria have a more dominant role in shaping the overall microbiota than fungi. Notably, each type of ham exhibited a unique microbial profile, although those from similar regions shared more similar profiles. Specific bacterial biomarkers were identified for each regional ham: <i>Lactobacillus</i> and <i>Tetragonococcus</i> in Serrano prosciutto, <i>Odoribacter</i>, <i>Alistipes</i>, <i>Staphylococcus</i>, and <i>Akkermansia</i> in Jinhua prosciutto, and <i>Pseudomonas</i>, <i>Blautia</i>, and <i>Bacteroides</i> in Xuanwei prosciutto. The microbial network analysis identified closer associations between microorganisms in the domestically produced Chinese hams than in the Spanish ham, suggesting limited foreign microbial invasions that contributed to a richer, more stable flavor. These findings offer new insights into how microbial interactions shape the development of flavor and quality in ham and clarify future strategies for improving the production process by leveraging microbial communities.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1531005"},"PeriodicalIF":4.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlorogenic acid alleviates the intestinal barrier dysfunction and intestinal microbiota disorder induced by cisplatin.","authors":"Ruiqi Tian, Yinchuan Ding, Shijie Zhang, Min Li, Yiran Wang, Qi Wu, Huanhuan Ding, Chengjie Song, Ce Shi, Min Xue","doi":"10.3389/fmicb.2025.1508891","DOIUrl":"10.3389/fmicb.2025.1508891","url":null,"abstract":"<p><strong>Introduction: </strong>The intestinal mucosal barrier is an important line of defense for the body, protecting it from intestinal bacteria, endotoxins, and antigens. Cisplatin, a clinical important chemotherapy medicine, is found the side effect with impairing intestinal epithelial cells' structure and function, even causes intestinal mucositis which causes patients immense suffering and hinders the process of cancer treatment. Chlorogenic acid, as the component only second to caffeine in coffee, has been proved the contribution on cardiovascular and gastrointestinal benefits. So, we investigate the protective effect of chlorogenic acid on cisplatin induced intestinal barrier structure and function injury in mice from the perspective of gut microbiota.</p><p><strong>Methods: </strong>C57BL/6J mice were divided into 4 groups, including the control group, a cisplatin group, a chlorogenic acid treatment group receiving intraperitoneal injections alongside cisplatin (Cis + CGA1), and the last group pre-treated with chlorogenic acid before cisplatin administration (Cis + CGA2). The inflammation factor of IL-6, IL-1β, and TNF-α in colonic tissue and serum were detected, respectively. To explore the protection of chlorogenic acid on mucosal barrier's integrity, we also detected the fecal LPS and the expression of occludin and ZO-1 proteins in colon tissue. And H&E staining was used to study the histopathological conditions of the colon tissue. Moreover, this article also utilized16S rDNA sequencing to analyze the gut microbiota of feces.</p><p><strong>Results: </strong>Chlorogenic acid administration reduced IL-6, IL-1β, and TNF-α level in both colon tissue and serum compared to the cisplatin alone treatment group. Furthermore, chlorogenic acid pretreatment notably improved intestinal barrier integrity by enhancing the expression of occludin and ZO-1 proteins in colon tissues. Moreover, 16S rDNA sequencing showed that compared with the control group, cisplatin group showed a reduced microbiota diversity, elevating abundance of Proteobacteria and pro-inflammatory environment of the increased Firmicutes/Bacteroidetes (F/B) ratio. However, chlorogenic acid treatment especially the pretreatment reversed the reduced microbiota diversity, elevating abundance of Proteobacteria and F/B ratio.</p><p><strong>Discussion: </strong>Microbiota diversity and all results suggest that chlorogenic acid treatment was able to mitigate these intestinal microbiota disorder and diversity reduction induced by cisplatin, effectively offer a protective effect against the inflammatory response and destruction of the mucosal barrier in the intestines caused by cisplatin.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1508891"},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}