Frontiers in Microbiology最新文献

{"title":"Genomic analysis of carbapenem-resistant <i>Klebsiella pneumoniae</i> blood isolates from nationwide surveillance in South Korea.","authors":"Younggwon On, Jung Wook Kim, Juyoung Lee, Jung Sik Yoo","doi":"10.3389/fmicb.2025.1562222","DOIUrl":"10.3389/fmicb.2025.1562222","url":null,"abstract":"<p><strong>Introduction: </strong>Carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP) poses a significant threat to public health owing to its multidrug resistance and rapid dissemination.</p><p><strong>Methods: </strong>This study analyzed CRKP isolates collected from bloodstream infections in nine regions of South Korea using the Kor-GLASS surveillance system between 2017 and 2021.</p><p><strong>Results: </strong>A total of 3,941 <i>K. pneumoniae</i> isolates were collected. Among them, 119 (3%) isolates were identified as CRKP. Most CRKP (79.7%) belonged to sequence type 307 (ST307), followed by ST11 (6.8%). All CRKP isolates exhibited multidrug resistance, with 78.8% carrying the IncX3 plasmid encoding the <i>KPC-2</i> gene. Phylogenetic and genomic analyses revealed that ST307 isolates exhibited low single nucleotide polymorphism (SNP) differences. SNP differences among ST307 strains ranged from a minimum of 1 to a maximum of 140, indicating close genetic relatedness. All ST307 strains harbored the KL102 and O1/O2v2 loci, and genomic analysis revealed high prevalence of key resistance genes such as <i>KPC</i> (91.5%) and <i>CTX-M-15</i> (83.9%), alongside mutations in the <i>QRDR</i> (ParC-80I, GyrA-83I) and <i>ompK</i> genes. Two major clusters were identified, with cluster 1 harboring yersiniabactin lineage 16 (ICEkp12) and cluster 2 showing higher virulence, including the yersiniabactin lineage 17 (ICEkp10) and colibactin-associated genes.</p><p><strong>Discussion: </strong>These findings underscore the dominance of ST307 among CRKP isolates in Korea, which is driven by clonal expansion and the critical role of mobile genetic elements. Therefore, enhanced genomic surveillance and targeted infection control measures are urgently required to address the spread of CRKP in clinical settings.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1562222"},"PeriodicalIF":4.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opinion: Commensal papillomavirus immunity preserves the homeostasis of highly mutated normal skin.","authors":"Nicholas A Wallace, Baki Akgül","doi":"10.3389/fmicb.2025.1576308","DOIUrl":"10.3389/fmicb.2025.1576308","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1576308"},"PeriodicalIF":4.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12087012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnology revival: <i>in situ</i> sludge minimization in wastewater.","authors":"Yiqiang Chen, Xu Jiang, Maosheng Yang, Zhu Wang","doi":"10.3389/fmicb.2025.1603215","DOIUrl":"10.3389/fmicb.2025.1603215","url":null,"abstract":"<p><p>In the face of the escalating challenge of sludge production and disposal in wastewater treatment plants (WWTPs), <i>in situ</i> sludge reduction biotechnology (ISRB) has recently emerged as a highly promising strategy. It not only has the potential to curtail sludge generation at its origin but also ensures the sustained efficiency of the treatment process. Several key strategies have demonstrated exceptional potential in harnessing microbial processes for sludge degradation. They encompass enzymatic hydrolysis, microbial inoculation, protozoan/metazoan predation, bacteriophage lysis, and biofilm-based manipulation. Compared to traditional methods (e.g., incineration and landfilling), these biotechnologies offer significant advantages through lower costs, reduced energy consumption, and minimal environmental impacts. The efficacy of ISRB is substantially affected by various factors, where pH, microbial shift, and nutrient conditions play crucial roles. Despite the notable progress made in this field, significant challenges persist when it comes to scaling up these technologies for more extensive and widespread applications. This review comprehensively highlights the fundamental mechanisms, application strategies, and future prospects of ISRB, including one of the first studies to introduce bacteriophage-based approaches for <i>in situ</i> sludge reduction, offering a novel perspective on phage-mediated sludge control. By doing so, it aims to offer in-depth insights into the role of ISRB as a sustainable solution for sludge management, paving the way for further research and development in this crucial area of environmental biotechnology.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1603215"},"PeriodicalIF":4.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizospheric <i>Bacillus</i> isolates control Fusarium wilt on cotton and enhance plant biomass and root development.","authors":"Seema Aslam, Muhammad Baber, Tahir Naqqash, Muhammad Javed, Sandra Bredenbruch, Florian M W Grundler, A Sylvia S Schleker","doi":"10.3389/fmicb.2025.1580937","DOIUrl":"10.3389/fmicb.2025.1580937","url":null,"abstract":"<p><p>Cotton is a globally significant crop, serving as a source of natural fiber for the textile industry and contributing to various other products. Its economic importance is substantial, impacting livelihoods and international trade. However, cotton production faces numerous challenges, including Fusarium wilt caused by <i>Fusarium oxysporum</i> f. sp. <i>vasinfectum</i> (Fov), which can lead to significant yield and fiber quality losses. Plants alter their root exudate profiles in response to pathogens, often selectively enriching for beneficial rhizobacteria with antagonistic activity and plant growth-promoting traits. This study thus aims to characterize bacteria isolated from the rhizosphere of diseased cotton plants. The antifungal activity of 43 isolates was assessed against Fov <i>in vitro</i>. Eight of these inhibited Fov growth by 68.4 to 76.9%. 16S rRNA sequencing confirmed these isolates as <i>Bacillus</i> species. These eight <i>Bacillus</i> strains were further examined for their different modes of action <i>in vitro</i>, and their effect on cotton plants in greenhouse experiments challenged with Fov. All eight strains produced chitinases and pectinases, seven demonstrated cellulase and three protease activity, six produced urease, and five siderophores. Only <i>B. subtilis</i> SC11 exhibited phosphate solubilization activity. Seed treatments revealed that <i>B. subtilis</i> SC10 and <i>B. subtilis</i> SC11 were the standout treatments reducing Fov-caused symptoms by ~83% compared to Fov-inoculated control plants and most significantly improved plant growth and antioxidant activity. In detail, <i>B. subtilis</i> SC11 increased shoot and root dry weight by 160 and 250%, respectively. <i>B. subtilis</i> SC10 increased peroxidase activity by ~143% and ascorbate peroxidase activity by ~60%, while in <i>B. subtilis</i> SC11 treated plants superoxide dismutase activity increased by ~100%. <i>Bacillus</i> treatments effectively mitigated lipid peroxidation, achieving up to 91.4% reduction (<i>B. subtilis</i> SC10, <i>B. halotolerans</i> SC15), and decreased H₂O₂ accumulation by up to 58.4% (<i>B. halotolerans</i> SC32) compared to the Fov control. Principle component analysis revealed that regarding plant growth parameters, the treatments, and controls were distributed differentially across PC1 and PC2, with 60.30 and 15.62% data variance, respectively, showing the effectiveness of <i>Bacillus</i> isolates in greenhouse experiments. The findings of this study will contribute to the development of sustainable biocontrol strategies for managing Fusarium wilt in cotton.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1580937"},"PeriodicalIF":4.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the impact of 16S rRNA gene intergenomic variation on primer design and gut microbiome profiling.","authors":"Sirinthorn Sunthornthummas, Rujipat Wasitthankasem, Pimonwan Phokhaphan, Nirinya Sudtachat, Alisa Wilantho, Chumpol Ngamphiw, Wanwisa Chareanchim, Sissades Tongsima","doi":"10.3389/fmicb.2025.1573920","DOIUrl":"10.3389/fmicb.2025.1573920","url":null,"abstract":"<p><p>The 16S rRNA gene is crucial for bacterial identification, but primer biases and intergenomic variation can compromise its effectiveness, especially in complex ecosystems like the human gut microbiome. This study systematically evaluates 57 commonly used 16S rRNA primer sets through <i>in silico</i> PCR simulations against the SILVA database. We identified three promising primer sets (V3_P3, V3_P7, and V4_P10) that offer balanced coverage and specificity across 20 key genera of the core gut microbiome. Our findings reveal: (1) significant limitations in widely used \"universal\" primers, often failing to capture microbial diversity due to unexpected variability in conserved regions, (2) substantial intergenomic variation, even within traditionally conserved regions of the 16S rRNA gene, as demonstrated by Shannon entropy analysis, and (3) discrepancies between intergenomic patterns in NCBI and SILVA databases, highlighting the impact of database choices on taxonomic classification. These results challenge assumptions about 16S rRNA gene conservation and emphasize the need for tailored primer design informed by comprehensive sequence databases. We advocate for a multi-primer strategy to improve coverage and mitigate biases, ultimately enhancing the accuracy and reliability of gut microbiome profiling. This approach has potential applications beyond gut microbiome studies, including animal microbiome research and probiotic community profiling.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1573920"},"PeriodicalIF":4.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming non-conventional yeasts into key players in biotechnology: advances in synthetic biology applications.","authors":"Soo Young Moon, Nan-Yeong An, Ju Young Lee","doi":"10.3389/fmicb.2025.1600187","DOIUrl":"10.3389/fmicb.2025.1600187","url":null,"abstract":"<p><p>Non-conventional yeasts exhibit exceptional genetic and functional diversity, serving as a largely untapped repertoire for biotechnological applications. Beyond the conventional yeast <i>Saccharomyces cerevisiae</i>, non-conventional yeasts are naturally more multifaceted, possessing the ability to utilize renewable and low-cost carbon sources while exhibiting robust physiology under challenging conditions. However, their vast potential remains largely unexplored, encompassing both challenges and opportunities for biotechnological advancements. Over the past decade, technological advancements in synthetic biology have unlocked new opportunities to harness their potential and overcome inherent limitations, enabling the full exploitation of their advantages across a broad spectrum of applications. In this review, we highlight recent advances in the synthetic biology of non-conventional yeasts, focusing on the development of new genetic building blocks (e.g., promoters and terminators), genome editing tools, and metabolic pathway engineering. Through these technologies, non-conventional yeasts are poised to emerge as pivotal next-generation workhorses tailored for specific applications in sustainable biomanufacturing, accelerating the transition to a bio-based economy.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1600187"},"PeriodicalIF":4.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking tripartite interaction dynamics: isolation, integration, and influence of bacteriophages in the <i>Paraburkholderia-Dictyostelium discoideum</i> symbiosis system.","authors":"Susanne DiSalvo, Negar Maness, Andrew Braun, My Tran, Andrew Hofferkamp","doi":"10.3389/fmicb.2025.1537073","DOIUrl":"10.3389/fmicb.2025.1537073","url":null,"abstract":"<p><strong>Introduction: </strong>Bacteriophages influence interactions between bacterial symbionts and their hosts by exerting parasitic pressure on symbiont populations and facilitating bacterial evolution through selection, gene exchange, and prophage integration. Host organisms also modulate phage-bacteria interactions, with host-specific contexts potentially limiting or promoting phage access to bacterial symbionts or driving alternative phenotypic or evolutionary outcomes.</p><p><strong>Methods: </strong>To better elucidate tripartite phage-bacteria-host interactions in real-time, we expanded the <i>Dictyostelium discoideum-Paraburkholderia</i> symbiosis system to include <i>Paraburkholderia</i>-specific phages. We isolated six environmental <i>Paraburkholderia</i> phages from soil samples using a multi-host enrichment approach. We also identified a functional prophage from monocultures of one of the <i>Paraburkholderia</i> symbiont strains implemented in the enrichment approach. These phages were evaluated across all three amoeba-associated <i>Paraburkholderia</i> symbiont species. Finally, we treated <i>Paraburkholderia</i> infected amoeba lines with select phage isolates and assessed their effects on symbiont prevalence and host fitness.</p><p><strong>Results: </strong>The isolated phages exhibited diverse plaquing characteristics and virion morphologies, collectively targeting <i>Paraburkholderia</i> strains belonging to each of the amoeba-symbiotic species. Following amoeba treatment experiments, we observed that phage application in some cases reduced symbiont infection prevalence and alleviated host fitness impacts, while in others, no significant effects were noted. Notably, phages were able to persist within the symbiont-infected amoeba populations over multiple culture transfers, indicating potential long-term interactions.</p><p><strong>Discussion: </strong>These findings highlight the variability of phage-symbiont interactions within a host environment and underscore the complex nature of phage treatment outcomes. The observed variability lays the foundation for future studies exploring the long-term dynamics of tripartite systems, suggesting potential mechanisms that may shape differential phage treatment outcomes and presenting valuable avenues for future investigation.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1537073"},"PeriodicalIF":4.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A metaproteomic analysis of the piglet fecal microbiome across the weaning transition.","authors":"Israel Rivera, KaLynn Harlow, Robert N Cole, Robert O'Meally, Wesley Garrett, Weili Xiong, William Oliver, James E Wells, Katie Lynn Summers, Nisan Chhetri, Olga Postnikova, Lea Rempel, Matt Crouse, Bryan Neville, Cary Pirone Davies","doi":"10.3389/fmicb.2025.1504433","DOIUrl":"10.3389/fmicb.2025.1504433","url":null,"abstract":"<p><p>Microbiome analysis has relied largely on metagenomics to characterize microbial populations and predict their functions. Here, we used a metaproteomic analysis of the fecal microbiome in piglets before and after weaning to compare protein abundances as they pertain to microbial populations specific to either a milk- or plant-based diet. Fecal samples were collected from six piglets on the day of weaning and 4 weeks after transitioning to a standard nursery diet. Using the 12,554 protein groups identified in samples, we confirmed the shift in protein composition that takes place in response to the microbial succession following weaning and demonstrated the redundancy in metabolic processes between taxa. We identified taxa with roles as primary degraders based on corresponding proteins synthesized, thereby providing evidence for cross-feeding. Proteins associated with the breakdown of milk-specific carbohydrates were common among pre-weaned pigs, whereas the proteome of post-weaned piglets contained a greater abundance of proteins involved in the breaking down plant-specific carbohydrates. Furthermore, output revealed that production of propionate takes place via the propionaldehyde pathway in pre-weaned piglets, but changes to production via the succinate pathway in post-weaned piglets. Finally, a disproportionate quantity of carbohydrate-active enzymes (CAZymes) (~8%) were produced by fungi, which typically only represent ~0.1% of the microbiome taxa. Information gathered through this characterization of the metaproteome before and after weaning revealed important differences regarding the role of members in the microbial community, thereby providing information for the optimization of diets and products for both piglet and microbiome health.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1504433"},"PeriodicalIF":4.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric analysis of hotspots in <i>Staphylococcus aureus</i> drug resistance research.","authors":"Jianguo Shi","doi":"10.3389/fmicb.2025.1572400","DOIUrl":"10.3389/fmicb.2025.1572400","url":null,"abstract":"<p><strong>Objective: </strong>Bibliometric analysis was used to visualize the current literature data on <i>Staphylococcus aureus</i> drug resistance.</p><p><strong>Methods: </strong>We used a bibliometrix package that utilizes R 4.4.1, all <i>S. aureus</i> drug resistance related research literature in the WOS core database, and data visualization and analysis of the retrieved research literature.</p><p><strong>Results: </strong>A total of 3,253 research articles on <i>Staphylococcus aureus</i> resistance were screened, and the number of publications related to <i>Staphylococcus aureus</i> resistance is increasing year by year. China ranks first in the number of publications, and the United States ranks first in terms of citations. Frontiers in microbiology has published the most articles on this research topic. Wang Y, Lee JH, and Simoes M have published the most articles, and Franci G has the most citations. The Egyptian Knowledge Bank (EKB), the Chinese Academy of Sciences, and Harvard University are the main research institutes. A total of 15,824 authors have contributed to the field, with the majority of authors coming from China, followed by South Korea and the United States, and most of them coming from the Chinese Academy of Science and Jilin University. The mechanism of drug resistance in <i>Staphylococcus aureus</i> was the most important research hotspot, with <i>Staphylococcus aureus</i> coming up 984 times, (10%), followed by antibiotic-resistance (693 times, 7%) and biofilm formation (517 times, 5%).</p><p><strong>Conclusion: </strong>This study comprehensively summarizes past research trends in <i>Staphylococcus aureus</i> resistance and identifies the countries, institutions, authors, journals, and publications involved in this field. The results provide a comprehensive overview of the study of resistance in <i>Staphylococcus aureus</i>.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1572400"},"PeriodicalIF":4.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082710/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of intestinal microbiota and its metabolites in the occurrence and intervention of obesity.","authors":"Yang Gao, Wenhui Liu, Xue Ma, Min Xue, Yingying Li, Shanshan Bi, Huan Leng","doi":"10.3389/fmicb.2025.1559178","DOIUrl":"10.3389/fmicb.2025.1559178","url":null,"abstract":"<p><p>Obesity is a growing global health challenge influenced by genetic and environmental factors. With economic development, obesity rates continue to rise globally. Recent research highlights the critical role of gut microbiota in obesity pathogenesis, particularly through microbial metabolites affecting metabolism and energy homeostasis. Recent studies indicate that gut microbiota significantly influences obesity development through metabolic interactions, including the regulation of glucose and lipid metabolism. Gut microbiota is involved in the metabolism of various nutrients such as glucose and lipids, and the change of gut microbiota leads to insulin resistance, adipose tissue accumulation and metabolic disorders, thus mediating the occurrence and development of metabolic diseases. For example, beneficial bacteria such as <i>Bifidobacterium</i> and <i>Lactobacillus</i>, which are in high abundance in animal intestinal tract, can prevent obesity by enhancing the intestinal barrier, improving insulin sensitivity and improving metabolic disorders. Many of these effects are mediated by metabolites produced by intestinal microbiota, such as short-chain fatty acids, which play an important role in preventing and treating obesity. In this research review, we used the PubMed database and Google Scholar and web of science to search for relevant literature within recent 20 years on the topic of microbes and microbial metabolites in relation to obesity. Key words used were obesity, metabolic diseases, gut microbiota, metabolic disorders, gut microbial metabolites to search for references. The purpose of this review article was to describe the current knowledge on the impact of gut microbiota and their metabolites on obesity, in order to provide a more comprehensive understanding of the relationship between gut microbiota and obesity for future clinical intervention and prevention of obesity.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1559178"},"PeriodicalIF":4.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}