Frontiers in Microbiology最新文献

{"title":"Pyrochar versus hydrochar: contrasting modulation of soil bacterial communities under severe nitrate-dominated acid rain stress in a <i>Quercus acutissima</i> plantation.","authors":"Yuanyuan Feng, Shushu Yao, Ke Yuan, Haibo Hu, Peng Cui, Yanfang Feng, Lihong Xue","doi":"10.3389/fmicb.2026.1961661","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1961661","url":null,"abstract":"<p><p>Nitrate-dominated acid rain (NAR) has become an increasingly prevalent component of contemporary atmospheric deposition and can profoundly alter soil bacterial communities in forest ecosystems. Biochar amendment has been proposed as a potential strategy for modifying soil conditions under acid deposition stress. However, the material-dependent effects of pyrochar (PC) and hydrochar (HC) on bacterial community responses under severe NAR stress remain unclear. Here, a field experiment was conducted in a <i>Quercus acutissima</i> plantation to compare the effects of PC and HC on soil bacterial community structure and predicted functional potential under simulated severe NAR stress. Compared with the NAR-only treatment, PC and HC reduced the relative abundance of Chloroflexi by 41.20 and 38.06%, respectively, and increased Actinobacteriota by 43.30 and 38.52%. LEfSe analysis revealed Chloroflexi as a phylum showing differential enrichment under NAR stress, whereas Acidobacteriota and Proteobacteria were differentially enriched in PC-NAR and HC-NAR treatments, respectively. Predicted phenotypic and functional profiles further indicated different bacterial responses to PC and HC. Exploratory association analysis suggested that PC-associated responses were more closely related to microbial biomass-associated variables, whereas HC-associated responses showed stronger relationships with soil chemical properties and microbial biomass. These findings highlight the material-dependent roles of PC and HC as carbon-based amendments and provide field-based insights into selecting biochar types for managing bacterial community responses in NAR-affected plantation soils.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1961661"},"PeriodicalIF":5.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13658203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and preliminary validation of an <i>omp25</i>-based RPA-CRISPR/Cas12a visual assay for rapid screening of brucellosis-associated <i>Brucella</i> DNA.","authors":"Yu Zhang, Shuairan Zhang, Cai Yin, Minjing Jin, Xiaona Zang, Yawen Wu, Taotao Bai, Long Ma, Xiaoliang Wang, Yong Shi, Zhixin Li","doi":"10.3389/fmicb.2026.1986052","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1986052","url":null,"abstract":"<p><p>Brucellosis remains an important zoonotic disease for livestock production and public health, creating a need for rapid molecular screening methods with limited equipment requirements. We developed a visual recombinase polymerase amplification (RPA)-CRISPR/Cas12a assay targeting a 642-bp region of the <i>Brucella omp25</i> gene. Comparative sequence analysis was used to assess target conservation, after which four RPA primer pairs and two CRISPR RNAs (crRNAs) were experimentally screened. RPA3-crRNA1 produced the strongest fluorescence response and was used for assay optimization. The final Cas12a reaction contained 250 nM Cas12a, 200 nM crRNA, and 200 nM single-stranded DNA fluorescent reporter. RPA amplification and Cas12a detection required 20 min each, and positive reactions produced visible yellow-green fluorescence under 470-nm blue light. No cross-reactivity was observed with the non-target pathogens included in the experimental panel. In silico comparisons with selected phylogenetically related taxa identified sequence differences within the final RPA3 primer-binding sites and crRNA1 recognition region, supporting a low predicted risk of sequence-based cross-reactivity for the taxa examined. Using purified plasmid DNA, the lowest DNA concentration detected under the experimental conditions tested was 2.72 copies/μL of input template, corresponding to an estimated 13.6 copies per RPA reaction. Among 96 bovine nasal swab samples collected during routine surveillance, agreement analysis against the qPCR comparator showed concordant results for 93 of 96 samples, with positive percent agreement (PPA), negative percent agreement (NPA), and overall percent agreement (OPA) of 100.00, 93.75, and 96.88%, respectively; three qPCR-negative samples showed weak RPA-CRISPR/Cas12a fluorescence and could not be independently resolved. Because the target region is conserved in the vaccine strains A19, S19, and RB51, the assay is intended for preliminary screening of brucellosis-associated <i>Brucella</i> DNA rather than species identification or differentiation of vaccine-derived and wild-type DNA. Broader validation with additional specimen types and specificity panels is required before routine diagnostic application.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1986052"},"PeriodicalIF":5.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13658145/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The gut microbiome and metabolome of people living with HIV altered during treatment.","authors":"Xue Ding, Jiahe Li, Pengyu Qian, Jingyu Yue, Xiuxia Ma, Liran Xu","doi":"10.3389/fmicb.2026.1920281","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1920281","url":null,"abstract":"<p><strong>Background: </strong>Despite effective viral suppression with antiretroviral therapy (ART), people living with HIV (PLWH) often experience persistent immune activation and incomplete immune reconstitution. Gut barrier disruption and gut microbial dysbiosis contribute to chronic inflammation in PLWH. Shen Ling Ai Cao pill (SLAC) may have immunomodulatory effects, however, changes in the gut microbiota and fecal metabolome during SLAC administration remain unclear. This study aimed to characterize changes in the gut microbiota and fecal metabolome during SLAC administration in PLWH.</p><p><strong>Methods: </strong>In this single-arm study, 46 PLWH on long-term ART received adjunctive SLAC for 6 months. Fecal samples were collected, and peripheral CD4+ and CD8+ T-cell counts and the CD4/CD8 ratio were measured at baseline and month 6. Gut microbiota were profiled by 16S rRNA gene sequencing, and untargeted metabolomics was performed to characterize fecal metabolic changes. Microbial diversity, differential taxa, functional pathways, differential metabolites, and KEGG enrichment were analyzed. Correlation analyses were conducted to explore associations among gut microbiota, metabolites, and T-cell subsets.</p><p><strong>Results: </strong>Among the 46 PLWH enrolled, 42 completed the 6-month follow-up and were included in the final analysis. From M0 to M6, median CD8+ T-cell counts increased from 448.5 to 565.0 cells/mm³ (<i>P</i> = 0.047), whereas median CD4+ T-cell counts increased non-significantly from 308.0 to 330.0 cells/mm³ (<i>P</i> = 0.118), with no change in the CD4/CD8 ratio (<i>P</i> = 0.89). Differences were also observed in gut microbial composition and fecal metabolomic profiles. Correlation analyses identified associations between gut microbial taxa and CD4+ and CD8+ T-cell counts. A total of 59 differential metabolites were identified, and enrichment analysis implicated lipid metabolism, bile acid metabolism, cholesterol metabolism, and mTOR signaling.</p><p><strong>Conclusions: </strong>In this single-arm longitudinal study of ART-treated PLWH, 6 months of adjunctive SLAC administration was associated with changes in fecal microbial composition, fecal metabolomic profiles, and CD8+ T cell counts, whereas CD4+ T cell counts showed a nonsignificant increasing trend. Controlled studies are needed to determine whether these changes are attributable to SLAC.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1920281"},"PeriodicalIF":5.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13658518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperinflammation and immunomodulation in influenza, SARS-CoV-2 and syncytial respiratory virus infection in adults: from innate immune responses and biomarkers to clinical and therapeutic implications.","authors":"Jesus Alonso-Carrillo, Marta Obra, Mario Cereza, María Ruiz-Ruigomez, Carlos Lumbreras, Antonio Lalueza","doi":"10.3389/fmicb.2026.1916526","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1916526","url":null,"abstract":"<p><p>Influenza, SARS-CoV2-2 and syncytial respiratory virus infection in adults are major causes of morbidity and mortality. However, the pathophysiological mechanisms underlying these infections remain incompletely understood. Disease severity results from an interaction between viral factors and host responses. Lung damage and respiratory failure are produced by hyperinflammation. Understanding these mechanisms is necessary to improve risk stratification and may guide therapeutic decisions. C- reactive protein (CRP), IL-6, ferritin, and ApoH are relevant biomarkers which can identify inflammatory phenotypes. Antivirals and supportive care are the main approaches for these viral infections. From a therapeutic perspective, broad immunosuppression is unlikely to be optimal, and strategies should focus on selectively modulating harmful inflammation while preserving antiviral immunity. Nevertheless, there is scarce evidence of the best choice of treatment based on the patient's clinical profile and further studies are needed.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1916526"},"PeriodicalIF":5.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13660187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving beef quality through rumen microbial modulation by varying alfalfa hay proportions in the diet.","authors":"Boshuai Liu, Yujie Zheng, Zimin Gao, Defeng Li, Xiaoyan Zhu, Hao Sun, Zhichang Wang, Yalei Cui, Yinghua Shi","doi":"10.3389/fmicb.2026.1961267","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1961267","url":null,"abstract":"<p><strong>Introduction: </strong>This study evaluated the effects of different dietary proportions of alfalfa hay on growth performance, carcass characteristics, meat quality, rumen fermentation, and rumen microbiota in beef cattle.</p><p><strong>Methods: </strong>Sixty Simmental crossbred cattle were randomly assigned to diets containing 11.57% (low alfalfa hay group, LAG), 20.83% (medium alfalfa hay group, MAG), or 30.09% (high alfalfa hay group, HAG) alfalfa hay, with four replicates per treatment and five cattle per replicate.</p><p><strong>Results: </strong>The LAG diet significantly increased final body weight and reduced beef shear force. The LAG diet also resulted in the highest saturated fatty acid content in the longissimus thoracis muscle. Based on 16S rRNA analysis, the LAG group had significantly higher relative abundances of Eubacterium_brachy_group and norank_f_Eubacterium_coprostanoligenes_group. Muscle n-6 polyunsaturated fatty acid content was positively correlated with the relative abundance of Christensenellaceae_R-7_group, whereas Succiniclasticum abundance was negatively correlated with the PICRUSt2-predicted acarbose and validamycin biosynthesis pathway.</p><p><strong>Discussion: </strong>A dietary alfalfa hay proportion of 11.57% improved final body weight and beef tenderness and was associated with distinct changes in rumen microbial composition and predicted microbial functions. These findings provide a scientific basis for using alfalfa hay to produce high-quality beef.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1961267"},"PeriodicalIF":5.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13658148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Confirmation of gamma irradiation-mediated inactivation of a vero-adapted African swine fever virus Lisbon 60 strain for molecular assays.","authors":"Sekhar Kambakam, Julia Thomas, Tod Stuber, Ping Wu, Suelee Robbe-Austerman, Rachel Palinski","doi":"10.3389/fmicb.2026.1882729","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1882729","url":null,"abstract":"<p><p>African swine fever virus (ASFV), the etiologic agent of African swine fever (ASF), is a high-consequence pathogen requiring experiments to be conducted in containment in non-endemic countries, thereby restricting diagnostic development, the creation of reference standards, and proficiency testing (PT). Safe and reliable inactivation methods are essential to expand diagnostic capacity while preserving nucleic acid integrity for molecular assays in unaffected countries. This study employed gamma irradiation to achieve complete inactivation of ASFV without compromising downstream molecular detection, as gamma irradiation offers deep penetration and uniform dose delivery. ASFV-cell culture supernatants were subjected to gamma irradiation doses ranging from 2 to 50 kGy. Viral replication was evaluated using TCID50 and serial passages, revealing a consistent dose-dependent reduction in infectivity across increasing irradiation dose levels and a complete loss of ASFV infectivity at 30 and 50 kGy. Molecular detection remained unaffected at all of the tested doses as confirmed by qPCR Ct values and sequence identity of the <i>p</i>72 gene. Whole genome sequencing demonstrated >99% genome coverage and consistent read depth profiles across irradiated and non-irradiated samples, indicating preservation of genomic integrity at all tested doses. These findings demonstrate that gamma irradiation at 50 kGy fully inactivates ASFV-cell supernatants while maintaining nucleic acid quality suitable for molecular diagnostics. The resulting inactivated material meets quality assurance requirements for molecular reference standards and PT panels and can be safely distributed to laboratories outside high containment facilities, supporting broader diagnostic readiness and harmonization of ASFV testing.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1882729"},"PeriodicalIF":5.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrasting ecological responses of abundant and rare bacterial and fungal subcommunities along an alpine marsh wetland degradation gradient.","authors":"Shijia Zhou, Mingzhu Cao, Jiao Tang, Chen Chen, Ni Zhang, Tao Wu, Kelong Chen","doi":"10.3389/fmicb.2026.1970129","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1970129","url":null,"abstract":"<p><strong>Introduction: </strong>Abundant and rare microbial taxa may respond differently to wetland degradation, yet their relative contributions to community reorganization remain poorly resolved in alpine marsh wetlands.</p><p><strong>Methods: </strong>We investigated lightly (LD), moderately (MD), and severely degraded (SD) alpine marsh wetlands on the northern shore of Qinghai Lake using 15 soil metagenomes and nine soil physicochemical properties. Bacterial and fungal genera were classified as abundant, intermediate, rare, or low-prevalence taxa according to domain-specific prevalence and mean relative abundance. Sample-level richness and original abundance contributions, alpha and beta diversity, community composition, distribution breadth, candidate indicator genera, and environmental associations were compared across the degradation gradient.</p><p><strong>Results: </strong>Total detected bacterial richness declined from 346.2 genera per sample in LD to 287.0 in SD. The rare bacterial share of detected richness decreased from 45.3% to 40.4%, while its original abundance contribution decreased from 6.79% to 3.70%. Abundant fungi accounted for 97.1-98.5% of total fungal abundance. Rare bacterial richness declined monotonically with degradation, whereas Shannon diversity, Simpson diversity, and Pielou's evenness declined in abundant fungi. All four focal subcommunities differed significantly in composition among degradation levels, with the largest PERMANOVA effect observed for abundant fungi and the smallest for rare fungi. Rare fractions exhibited greater incidence-based beta diversity and narrower observed distributions than abundant fractions. Soil PC1 and PC2 were associated with 51.6%, 41.4%, and 55.1% of the variation in abundant bacterial, rare bacterial, and abundant fungal communities, respectively, but with only 0.6% of the variation in rare fungal communities.</p><p><strong>Discussion: </strong>Partitioning microbial communities into abundant and rare fractions revealed pronounced abundance- and domain-dependent reorganization along the wetland degradation gradient. Rare bacteria showed marked losses in richness and abundance contribution, whereas abundant fungi exhibited stronger declines in within-community diversity and greater environmental structuring. These contrasting responses were obscured in whole-community summaries, highlighting the value of explicitly separating abundant and rare taxa when evaluating microbial responses to alpine marsh wetland degradation.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1970129"},"PeriodicalIF":5.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13658454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the gut-liver axis in MASLD: from dysbiosis-driven pathogenesis to precision microbiome therapeutics.","authors":"Wenxin Liu, Yi Lan, Xia Chen, Hui Wei, Tianjiao Zhang, Yuping Wang","doi":"10.3389/fmicb.2026.1971785","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1971785","url":null,"abstract":"<p><p>Metabolic dysfunction-associated steatotic liver disease (MASLD) has emerged as the most prevalent chronic liver disorder worldwide, characterized by a multifactorial pathogenesis involving genetic predisposition, metabolic disturbances, environmental influences, and gut microbiota interactions. In recent years, the gut microbiota has garnered significant attention for its pivotal role in MASLD development and progression through the gut-liver axis. This review comprehensively summarizes current understanding of the molecular mechanisms by which gut microbiota and their metabolites contribute to MASLD pathogenesis, including intestinal barrier dysfunction, bile acid metabolism dysregulation, abnormalities in short-chain fatty acid and tryptophan metabolism, oxidative stress, and mitochondrial impairment. Furthermore, we comprehensively discuss advances in therapeutic strategies targeting gut microbiota modulation, such as probiotics/prebiotics, fecal microbiota transplantation, dietary interventions, bioactive compounds from traditional Chinese medicine, and surgical approaches. By integrating these insights, this review aims to provide novel perspectives for precise prevention and treatment of MASLD, highlighting the gut microbiota as a promising target for future clinical management.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1971785"},"PeriodicalIF":5.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contact-mediated triclosan delivery for transmission blocking of <i>Plasmodium</i> in <i>Anopheles stephensi</i>.","authors":"Meenakshi Jeena, Gaurav Kumar, Bharti Goyal, Suman Lata, Lipun Kumar Pradhan, Yamini Thakur, Gaganpreet Kaur, Vaishali Verma, Kailash C Pandey, Shweta Pasi, Jaspreet Kaur","doi":"10.3389/fmicb.2026.1927723","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1927723","url":null,"abstract":"<p><strong>Introduction: </strong>Extensive and long-term insecticide use has led to the development of resistance in mosquito vectors, underscoring the need for novel insecticidal molecules and integrated, multifaceted strategies that simultaneously target both the vector and the parasite. In line with this, we evaluated the potential of a lipophilic drug triclosan, as a contact-mediated inhibitor to block the development of <i>Plasmodium</i> parasite within the malaria vector, <i>Anopheles stephensi</i>.</p><p><strong>Methods: </strong>Molecular docking and simulations identified the potential of triclosan to interact with the ookinete surface proteins of the <i>Plasmodium</i> species. Intrinsic toxicity of triclosan was first established on both larval and adult stages of laboratory reared <i>An. stephensi</i> through oral feeding and contact exposure (topical application and bottle bio-assay) in terms of survival and/or fecundity and egg hatchability. The transmission blocking potential of triclosan was initially tested <i>in vitro</i> using exflagellation assays and ookinete development assays. Finally, studies with <i>P. berghei</i> challenged mosquitoes were done to check the <i>in vivo</i> transmission blocking activity of triclosan through bottle bioassays.</p><p><strong>Results: </strong>Docking and simulations showed stable binding of triclosan to <i>Plasmodium</i> ookinete surface proteins. Contact exposure of triclosan was non-toxic for adult mosquitoes. However, parasite development was markedly affected. We observed a concentration dependent reduction in exflagellation centers and ookinete formation with increasing concentrations of triclosan compared to the control group. However, at 70 μM concentration, a significant reduction of 87% in exflagellation centers was observed. There was a substantial reduction in ookinete formation; at 50 μM, it was 97.7%, while at 70 μM, the reduction reached 99%. <i>In vivo</i>, triclosan exposure resulted in a substantial decrease in oocyst development, with reductions of up to 86% at higher concentrations.</p><p><strong>Conclusion: </strong>These findings demonstrate triclosan as a contact-mediated anti-<i>Plasmodium</i> agent that disrupts parasite development within the mosquito vector, underscoring its potential as a novel vector-targeted transmission blocking intervention against malaria.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1927723"},"PeriodicalIF":5.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13658491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An FTA card-based CRISPR/Cas12a platform with isothermal amplification for portable tuberculosis diagnosis.","authors":"Jiayin Xing, Xiangxiang Zhao, Gaojie Wang, Shilong Ren, Lei Chen, Wenbin Diao, Meihua Qu, Zhimin Chang, Xiaoqian Wang, Shihao Zhao, Yong Zhang, Ningning Song","doi":"10.3389/fmicb.2026.1958414","DOIUrl":"https://doi.org/10.3389/fmicb.2026.1958414","url":null,"abstract":"<p><p><i>Mycobacterium tuberculosis</i> remains a major global public health threat, imposing significant socioeconomic burdens. Current molecular diagnostics rely heavily on nucleic acid extraction and specialized equipment, which limits their use in resource-limited settings. We developed a rapid, sensitive platform suitable for point-of-care detection by integrating FTA cards with ERA/RT-ERA amplification and CRISPR/Cas12a, enabling direct analysis of samples without conventional nucleic acid purification. Leveraging FTA cards for efficient nucleic acid release and stable preservation, we established assays targeting IS6110, esxB, gyrB, and the RNA target ASdes. Integration of FTA-based sample processing, ERA/RT-ERA amplification, and Cas12a detection enables fast and reliable identification of nucleic acid markers. The detection limits were 12.5 pM for IS6110, 6.25 pM for esxB, 25 pM for gyrB, and 12.5 pM for ASdes. Our results showed strong concordance between endpoint fluorescence and qPCR results, demonstrating robust quantitative performance for both DNA and RNA targets. In clinical serum samples, the fluorescence readouts and lateral flow strip outputs showed strong concordance with quantitative PCR results across all four targets. Collectively, the FTA-ERA-CRISPR/Cas12a platform serves as a practical and offers a reliable approach for rapid tuberculosis diagnosis at the point of need. This integrated multiple distinct targets strategy provides a versatile framework for portable molecular diagnostics and holds promise for improving TB case detection in resource-limited settings.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"17 ","pages":"1958414"},"PeriodicalIF":5.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13658155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}