Frontiers in Microbiology最新文献

{"title":"Genomic insights into <i>bla</i> _AFM-positive carbapenem-resistant <i>Pseudomonas aeruginosa</i> in China.","authors":"Huimin Lu, Chuanjun Zhang, Buhui Zhao, Yan Li, Shangshang Qin","doi":"10.3389/fmicb.2025.1546662","DOIUrl":"10.3389/fmicb.2025.1546662","url":null,"abstract":"<p><p>Carbapenem-resistant <i>Pseudomonas aeruginosa</i> (CRPA) poses a global threat; however, the epidemiological characteristics and clinical significance of <i>bla</i> _AFM-positive CRPA strains in China remain unclear. In this study, continuous surveillance was conducted from 2018 to 2022 in a hospital in Henan Province, China, and the genomic characteristics of <i>bla</i> _AFM-positive CRPA were elucidated. We characterised the genetic features of <i>bla</i> _AFM-positive CRPA isolates by antimicrobial susceptibility testing, conjugation assays, whole-genome sequencing, large-scale comparative genomics, and bioinformatic analyses. Among 628 CRPA isolates, one <i>bla</i> _AFM-positive multidrug-resistant (MDR) strain, PA19-3158 (ST1123), was identified, with the <i>bla</i> _AFM-1 gene located on a novel 518,222 bp megaplasmid. Additionally, big data analysis revealed the genomic characteristics of <i>bla</i> _AFM-positive CRPA across China. A total of three different <i>bla</i> _AFM gene variants were identified among these isolates, namely <i>bla</i> _AFM-1 (44.12%), <i>bla</i> _AFM-2 (52.94%), and <i>bla</i> _AFM-4 (2.94%). Our findings identified ST463 as the dominant clone among <i>bla</i> _AFM-positive CRPA in different regions of China, with some <i>bla</i> _AFM-positive CRPA isolates from these regions exhibiting high genetic similarity. Notably, all <i>bla</i> _AFM-positive CRPA isolates carried multiple antibiotic resistance genes (ARGs), with approximately 38% co-harboring the carbapenem-resistant gene <i>bla</i> _KPC-2 and approximately 47% co-harboring the tigecycline-resistant gene <i>tmexCD-toprJ</i>. Correlation analysis underscored the significant role of mobile genetic elements in facilitating <i>bla</i> _AFM gene transfer. These results highlight the critical need for continuous surveillance of <i>bla</i> _AFM-positive CRPA in clinical settings to mitigate potential risks.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1546662"},"PeriodicalIF":4.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic power, volatile compounds, and flavor marker formation mechanisms of three fungi isolated from Fengxiangxing Daqu.","authors":"Dan Cao, Jiali Lv, Cui Liu, Chengyong Jin, Yongli Zhang, Yuhang Zhang, Wen Zhang","doi":"10.3389/fmicb.2025.1593638","DOIUrl":"10.3389/fmicb.2025.1593638","url":null,"abstract":"<p><strong>Introduction: </strong>Chinese Daqu is a naturally formed microbial complex, which is the fermenter of Chinese Baijiu. The microorganisms in Chinese Daqu determine the flavor of Chinese Baijiu.</p><p><strong>Methods: </strong>Based on PacBio SMRT ITS sequencing technology, fungal genome de novo sequencing, and Headspace Solid-Phase Micro-extraction/Gas Chromatography-Mass Spectrometry analyzed and identified three fungi, their abundance, volatile compounds, and flavor markers in Daqu.</p><p><strong>Results: </strong><i>Aspergillus chevalieri</i>, <i>Saccharomycopsis fibuligera</i>, and <i>Thermoascus aurantiacus</i> were the three most abundant fungi, with relative abundances of 5.702, 31.686, and 62.256%, respectively. <i>Aspergillus chevalieri</i> and <i>Saccharomycopsis fibuligera</i> were associated with volatile markers of chained terpene alcohols and lactones, with linalool and γ-nonanolactone identified as their flavor markers, respectively. <i>Thermoascus aurantiacus</i> produced the highest variety and content of pyrazine. According to the functional annotation and metabolic relationship of the whole genomes of <i>Aspergillus chevalieri</i> and <i>Saccharomycopsis fibuligera</i> in COG, KEGG and other databases, the formation mechanism and possible metabolic pathways of linalool and γ-nonanolactone were elucidated. The results of the study revealed the different contributions of the three dominant fungi to the volatile compounds of Daqu, can provide ideas for the study of the contribution of other microorganisms to the volatile compounds of Daqu and the mechanism. It can provide a basis for the precise adjustment of the flavor of Daqu, and then the precise modification of the flavor of Baijiu.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1593638"},"PeriodicalIF":4.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbapenem-resistant Gram-negative bacteria exhibiting clinically undetected cefiderocol heteroresistance leads to treatment failure in a murine model of infection.","authors":"Hongwei Liu, Peng Zhou, Peng Ma, Yaqin Liu, Yingfeng Zhang, Qiwei Li, Lingqing Xu, Wenchang Yuan, Weiguo Yin, Linhai Li, Yang Lu","doi":"10.3389/fmicb.2025.1496514","DOIUrl":"10.3389/fmicb.2025.1496514","url":null,"abstract":"<p><strong>Introduction: </strong>Carbapenem-resistant (CR) Gram-negative pathogens are classified by the WHO as critical threats due to limited therapeutic options. Cefiderocol (CFD), a novel siderophore cephalosporin, shows promise but remains unapproved in China. This study investigated the prevalence, clinical impact, and genetic mechanisms of cefiderocol heteroresistance (CFD-HR) in CR and ESBL-producing clinical isolates from China, where CFD remains unapproved.</p><p><strong>Methods: </strong>A total of 407 CR and ESBL-producing isolates were analyzed. CFD-HR was identified by population analysis profiles (PAPs). Clinical relevance was assessed through disk diffusion susceptibility testing, time-kill assays, and a murine peritonitis model. Genetic mechanisms and stability were elucidated by whole-genome sequencing (WGS) and fitness cost assays.</p><p><strong>Results: </strong>CFD-HR prevalence was 17.4% (16/92) in carbapenem-resistant <i>A. baumannii</i> (CRAB), 27.9% (24/86) in carbapenem-resistant <i>P. aeruginosa</i> (CRPA), 23.8% (10/42) in carbapenem-resistant <i>E. coli</i> (CRE), and ≤10% (1/10 in ESBL-producing <i>P. aeruginosa</i> and 8/177 in ESBL-producing <i>E. coli</i>). Although 72.9% (43/59) of HR isolates were classified as CFD-susceptible by disk diffusion, time-kill assays showed that 66.7% (4/6) of HR strains required ≥8 mg/L CFD (vs. 4 mg/L for non-HR) to prevent regrowth. <i>In vivo</i>, CFD achieved 100% (3/3) survival in non-HR infections but only 16.7% (4/6) in HR-infected mice. WGS identified transient genetic alterations in HR subpopulations, including <i>sitABCD</i> duplications (CRE), <i>oprD</i> mutations (CRAB), and <i>vgrG</i> SNPs (CRPA), which reverted after antibiotic withdrawal. Fitness cost assays revealed unstable growth deficits in 33.3% (2/6) of HR subpopulations, correlating with genetic instability.</p><p><strong>Discussion: </strong>These findings highlight the clinical significance of CFD-HR, even in susceptible isolates, and underscore the need for improved diagnostic methods to detect HR and monitor cross-resistance, offering critical insights for regions transitioning to CFD implementation.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1496514"},"PeriodicalIF":4.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracheal and cloacal bacterial diversity of red listed Eastern Imperial Eagle (<i>Aquila heliaca</i>).","authors":"Marine Murtskhvaladze, Levan Ninua, Nika Budagashvili, Ekaterine Tevdoradze, Zurab Gurgenidze, Adam Kotorashvili, Nato Kotaria, Alexander Gavashelishvili, Zurab Javakhishvili","doi":"10.3389/fmicb.2025.1477032","DOIUrl":"10.3389/fmicb.2025.1477032","url":null,"abstract":"<p><p>This study aimed to improve knowledge of raptor microbiomes by providing the first description of tracheal and cloacal bacterial diversity of Eastern Imperial Eagles (<i>Aquila heliaca</i>). To date, only few studies are available and they are carried out mainly on captive birds. The Eastern Imperial Eagle is species of significant conservation concern and, therefore, characterization microbiota contributes valuable information to the field of avian microbiology and aids in conservation efforts for this threatened species, moreover, identification of avian and human pathogens within microbial communities and evaluation of potential threats to birds, humans, and other species are crucial for sustainably balancing the wellbeing of ecosystems, 3,500 OTUs were identified from each sample supported by ∼2.8 Million sequence reads. The tracheal and cloacal microbiomes were dominated by <i>Gammaproteobacteria</i> (67.5%), <i>Bacilli</i> (43.8%), and <i>Negativicutes</i> (22.0%). We detected dissimilarities between cloacal (unique 440 OTUs) and tracheal (337 unique OTUs) samples, and significant evidence of moderate positive monotonic relationship between cloacal and tracheal bacterial communities. No significant differences between individuals from different nests. <i>Aquila heliaca</i> can serve as an indicator of presence of bacterial species in its respective habitats. Efforts aiming at protection of red-listed birds may not presently prioritize microbiome considerations but integrating microbiome research into conservation strategies could yield significant benefits.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1477032"},"PeriodicalIF":4.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined inactivation of the SOS response with TCA fumarases and the adaptive response enhances antibiotic susceptibility against <i>Escherichia coli</i>.","authors":"Marina Murillo-Torres, Isabel María Peñalver-Fernández, Marta Quero-Delgado, Sara Diaz-Diaz, María Romero-Muñoz, Esther Recacha, Fernando Docobo-Pérez, José Manuel Rodríguez-Martínez","doi":"10.3389/fmicb.2025.1570764","DOIUrl":"10.3389/fmicb.2025.1570764","url":null,"abstract":"<p><strong>Introduction: </strong>Targeting bacterial DNA damage responses such as the SOS response represents a promising strategy for enhancing the efficacy of existing antimicrobials. This study focuses on a recently discovered DNA damage response mechanism involving tricarboxylic acid cycle (TCA) fumarases and the adaptive response, crucial for <i>Escherichia coli</i> survival in the presence of genotoxic methyl methanesulfonate (MMS). We investigated whether this pathway contributes to protection against antibiotics, either separately or in combination with the SOS response.</p><p><strong>Methods: </strong>An isogenic collection of <i>E. coli</i> BW25113 mutants was used, including strains deficient in fumarases (Δ<i>fumA</i>, Δ<i>fumB</i>, Δ<i>fumC</i>) and the adaptive response (Δ<i>alkA</i>, Δ<i>alkB,</i> Δ<i>aidB</i>). Additional SOS response inactivation (Δ<i>recA</i>) was conducted by P1 phage transduction. All mutants were subjected to antimicrobial susceptibility testing, growth curve analysis, survival and evolution assays. To validate the relevance of these findings, experiments were also performed in a quinolone-resistant <i>E. coli</i> ST131 clinical isolate.</p><p><strong>Results and discussion: </strong>Overall, no significant differences or only moderate increases in susceptibility were observed in the single mutants, with Δ<i>fumC</i> and Δ<i>aidB</i> mutants showing the highest susceptibility. To enhance this effect, these genes were then inactivated in combination with the SOS response by constructing Δ<i>fumC</i>/Δ<i>recA</i> and Δ<i>aidB</i>/Δ<i>recA</i> mutants. These combinations exhibited significant differences in susceptibility to various antimicrobials, particularly cephalosporins and quinolones, and especially in the Δ<i>fumC</i>/Δ<i>recA</i> strain. To further assess these results, we constructed an <i>E. coli</i> ST131 Δ<i>fumC</i>/Δ<i>recA</i> mutant, in which a similar trend was observed. Together, these findings suggest that co-targeting the SOS response together with fumarases or the adaptive response could enhance the effectiveness of antibiotics against <i>E. coli</i>, potentially leading to new therapeutic strategies.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1570764"},"PeriodicalIF":4.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolism of oxyfluorfen by actinobacteria <i>Micrococcus</i> sp. F3Y.","authors":"Li Yao, Yue Wen, Yuting Sha, Leqin Wang, Xianrui Bi, Shuhan Si, Min Shen, Shusong Zhang, Haiyan Ni","doi":"10.3389/fmicb.2025.1599015","DOIUrl":"10.3389/fmicb.2025.1599015","url":null,"abstract":"<p><p>Oxyfluorfen, a potent diphenyl ether herbicide, has raised significant environmental concerns due to its persistence, toxicity to non‒target organisms, and potential carcinogenicity. Microbial degradation plays a crucial role in mitigating its impact, yet complete mineralization pathways remain poorly understood. In this study, we isolated <i>Micrococcus</i> sp. F3Y, an oxyfluorfen‒degrading actinobacterium, and evaluated its degradation efficiency in yeast powder‒supplemented mineral medium (YPM) medium and oxyfluorfen‒contaminated soil. Optimal conditions, pH, temperature, initial optical density (OD_600nm) were determined. Metabolites were analyzed via UPLC/Q‒TOF MS, and a putative gene cluster was identified through draft genome sequencing. Strain F3Y completely degraded 100 mg/L oxyfluorfen within 12 h under optimal conditions (pH 7.0, 30°C, OD600=2.0), maintaining over 55% efficiency at 25‒42°C and above 62% across a pH range of 6.5‒8.0. When the initial oxyfluorfen concentration was ≤150 mg/L, the degradation rate exceeded 74%. Moreover, in oxyfluorfen‒contaminated soil (0.06 mg/kg), inoculation with strain F3Y restored soybean (<i>Glycine max</i>) growth, increasing shoot length from 4.22 cm (severely inhibited) to 28.8 cm, a nearly 7‒fold improvement. Additionally, F3Y achieved 98.2% degradation of oxyfluorfen (50 mg/kg) within 25 d in unsterilized soil. Eleven metabolites, including six new intermediates, were identified. Based on these, two novel degradation pathways were proposed: one initiated by nitro reduction and the other by diaryl ether cleavage. Both pathways culminated in aromatic ring opening and complete mineralization. In addition, a potential 24.3 kb gene cluster, <i>pao</i>, was suggested. Comprising thirteen genes, it was hypothesized to participate in the ring cleavage of intermediate products during oxyfluorfen degradation. This study provided the first comprehensive evidence of <i>Micrococcus</i> mediated oxyfluorfen mineralization, offering new insights into actinobacterial metabolic versatility. With its high degradation efficiency, environmental resilience, and detoxification ability, F3Y was an ideal candidate for bioremediation. These finding not only enhanced the understanding of herbicide degradation but also provided a sustainable solution to address oxyfluorfen contamination in agricultural and natural ecosystems.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1599015"},"PeriodicalIF":4.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between pediatric asthma and respiratory microbiota, intestinal microbiota: a narrative review.","authors":"Lian Liu, Wenqi Zhao, Han Zhang, Yunxiao Shang, Wanjie Huang, Qi Cheng","doi":"10.3389/fmicb.2025.1550783","DOIUrl":"10.3389/fmicb.2025.1550783","url":null,"abstract":"<p><p>Pediatric asthma is a common chronic airway inflammatory disease that begins in childhood and its impact persists throughout all age stages of patients. With the continuous progress of detection technologies, numerous studies have firmly demonstrated that gut microbiota and respiratory microbiota are closely related to the occurrence and development of asthma, and related research is increasing day by day. This article elaborates in detail on the characteristics, composition of normal gut microbiota and lung microbiota at different ages and in different sites, as well as the connection of the gut-lung axis. Subsequently, it deeply analyzes various factors influencing microbiota colonization, including host factor, delivery mode, maternal dietary and infant feeding patterns, environmental microbial exposure and pollutants, and the use of antibiotics in early life. These factors are highly likely to play a crucial role in the onset process and disease progression of asthma. Research shows that obvious changes have occurred in the respiratory and gut microbiota of asthma patients, and these microbiomes exhibit different characteristics according to the phenotypes and endotypes of asthma. Finally, the article summarizes the microbiota-related treatment approaches for asthma carried out in recent years, including the application of probiotics, nutritional interventions, and fecal microbiota transplantation. These treatment modalities are expected to become new directions for future asthma treatment and bring new hope for solving the problem of childhood asthma.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1550783"},"PeriodicalIF":4.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the hidden allies of industrial chicory-a metagenomic exploration of rhizosphere microbiota and their impact on productivity and plant health.","authors":"Lalie Leclercq, Sony Debarre, Emily Lloret, Bernard Taminiau, Georges Daube, Caroline Rambaud, Djamel Drider, Ali Siah, Bruno Desprez, Jean-Louis Hilbert, Anca Lucau-Danila","doi":"10.3389/fmicb.2025.1509094","DOIUrl":"10.3389/fmicb.2025.1509094","url":null,"abstract":"<p><strong>Background: </strong>As industrial chicory is significant for food, fodder, and medicinal purposes, its cultivation is increasingly crucial for producers. To enhance productivity, resistance, and the nutritional and functional values of this plant, we aimed to investigate its interactions with the microbial environment. We performed the first comprehensive taxonomic and functional characterization of the rhizosphere microbiota associated with industrial chicory, investigating how environmental factors influence its composition.</p><p><strong>Methods: </strong>Six different land plots were simultaneously cultivated with the same chicory genotype in northern France. Using soil analyses and metagenomic approaches, we characterized the diversity of bacterial and fungal communities in the soil microbiome associated with chicory plants and discussed their functional traits.</p><p><strong>Results: </strong>We observed significant taxonomic variability, influenced by soil composition and cultivation history across each plot. The presence of chicory plants distinctly shaped the microbial community. Specifically, chicory was found to recruit <i>Streptomyces</i> species that produce plant hormones and <i>Penicillium</i> species that facilitate phosphate solubilization and promote plant growth. Moreover, the plant demonstrated an ability to repel pathogens and adapt to local microbial communities by selectively favoring beneficial microorganisms according to local stresses and nutritional needs.</p><p><strong>Discussion: </strong>Our study represents a comprehensive taxonomic and functional analysis of the <i>Cichorium intybus</i> rhizosphere microbiome, underscoring the pivotal role of soil composition and land-use history. The specific microbial recruitment by chicory was also addressed.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1509094"},"PeriodicalIF":4.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide expression in human whole blood for diagnosis of latent tuberculosis infection: a multicohort research.","authors":"Fan Jiang, Yanhua Liu, Linsheng Li, Ruizi Ni, Yajing An, Yufeng Li, Lingxia Zhang, Wenping Gong","doi":"10.3389/fmicb.2025.1584360","DOIUrl":"10.3389/fmicb.2025.1584360","url":null,"abstract":"<p><strong>Background: </strong>Tuberculosis (TB) remains a significant global health challenge, necessitating reliable biomarkers for differentiation between latent tuberculosis infection (LTBI) and active tuberculosis (ATB). This study aimed to identify blood-based biomarkers differentiating LTBI from ATB through multicohort analysis of public datasets.</p><p><strong>Methods: </strong>We systematically screened 18 datasets from the NIH Gene Expression Omnibus (GEO), ultimately including 11 cohorts comprising 2,758 patients across 8 countries/regions and 13 ethnicities. Cohorts were stratified into training (8 cohorts, <i>n</i> = 1,933) and validation sets (3 cohorts, <i>n</i> = 825) based on functional assignment.</p><p><strong>Results: </strong>Through Upset analysis, LASSO (Least Absolute Shrinkage and Selection Operator), SVM-RFE (Support Vector Machine Recursive Feature Elimination), and MCL (Markov Cluster Algorithm) clustering of protein-protein interaction networks, we identified S100A12 and S100A8 as optimal biomarkers. A Naive Bayes (NB) model incorporating these two markers demonstrated robust diagnostic performance: training set AUC: median = 0.8572 (inter-quartile range 0.8002, 0.8708), validation AUC = 0.5719 (0.51645, 0.7078), and subgroup AUC = 0.8635 (0.8212, 0.8946).</p><p><strong>Conclusion: </strong>Our multicohort analysis established an NB-based diagnostic model utilizing S100A12/S100A8, which maintains diagnostic accuracy across diverse geographic, ethnic, and clinical variables (including HIV co-infection), highlighting its potential for clinical translation in LTBI/ATB differentiation.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1584360"},"PeriodicalIF":4.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Clostridium Butyricum 337279</i> shapes the gut microbiota to attenuate metabolic disorder in diet-induced obese mice.","authors":"Xuejiao Zhang, Zhiyu Li, Junjun Cao, Haipeng Sun, Wenyan Niu","doi":"10.3389/fmicb.2025.1580847","DOIUrl":"10.3389/fmicb.2025.1580847","url":null,"abstract":"<p><strong>Aims: </strong>Obesity is one of the important challenges to public health worldwide. Dysbiotic intestinal microbiota is a key factor in the onset and progression of obesity and related diseases. Short chain fatty acids (SCFAs) derived from <i>butyricogenic bacteria</i> has beneficial effects on obesity. <i>Clostridium Butyricum 337279</i> (<i>C.B</i>), one of the SCFA producing bacteria, has been used to treat inflammatory bowel disease. The effect of <i>C.B</i> on obese mice remains unclear.</p><p><strong>Methods: </strong>A high fat diet (HFD)-induced mouse model of obesity was constructed, and the mice were treated with <i>C.B</i> to examine their role on obesity and related metabolic disorder. RT-qPCR, Western blotting, immunohistochemical staining, and 16S rRNA gene sequencing were performed to investigate the role and mechanism of <i>C.B</i>. Plasma levels of BCAA and BCKA were detected by Shimadzu LC-20 AD liquid chromatography (LC) system.</p><p><strong>Results: </strong>Here we demonstrated that oral administration of <i>C.B</i> effectively alleviated HFD-induced obesity and associated metabolic disorders, including glucose intolerance and hyperlipidemia, as well as systemic inflammation, as evidenced by reduced levels of LPS, TNF-<i>α</i>, and IL-1β. <i>C.B</i> alleviated intestinal flora imbalance and modulated the composition of gut microbiota and their metabolites in HFD-induced obese mice. It also significantly ameliorated intestinal barrier disorders by increasing protein level of tight junction proteins ZO-1 and occludin. Importantly, dietary <i>C.B</i> potentially suppressed bacterial biosynthesis of branched-chain amino acids (BCAA) and reduced the excessive accumulation of BCAA in plasma, suggesting its role in restoring BCAA metabolism of mice.</p><p><strong>Conclusion: </strong><i>C.B</i> intervention significantly ameliorated gut microbiota imbalance in obese mice and alleviated obesity-related metabolic disorders by upregulating the expression of tight junction proteins (ZO-1 and occludin), attenuating endotoxemia and systemic inflammation and reducing microbial-derived BCAA production.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1580847"},"PeriodicalIF":4.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098643/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}