Frontiers in Microbiology最新文献

{"title":"Exploring the potential role of microbiota and metabolites in acute exacerbation of chronic obstructive pulmonary disease.","authors":"Yanmin Shi, Jianya Yang, Tao Tian, Suyun Li, Yang Xie","doi":"10.3389/fmicb.2024.1487393","DOIUrl":"10.3389/fmicb.2024.1487393","url":null,"abstract":"<p><p>The acute exacerbation of chronic obstructive pulmonary disease seriously affects the respiratory system function and quality of life of patients. This study employed 16S rRNA sequencing and metabolomics techniques to analyze the respiratory microbiota and serum metabolites of COPD and AECOPD patients. The results showed that the microbial diversity in the respiratory tract of AECOPD patients was significantly lower than that of COPD patients, and the relative abundance of Bacteroidetes, <i>Prevotella</i> and <i>Neisseria</i> in the respiratory tract of AECOPD patients was significantly lower than that of COPD patients. However, the relative abundance of <i>Haemophilus_D</i>, <i>Veillonella_A</i> and <i>Pseudomonas_E</i>, in AECOPD patients was significantly higher than that of COPD patients, and the ability of respiratory microbiota in AECOPD patients to participate in alanine metabolism was significantly lower than that of COPD patients. Metabolome results further revealed that the serum alanine levels in AECOPD patients were significantly lower than those in COPD patients, and these differential metabolites were mainly involved in linoleic acid metabolism, protein digestion and absorption and regulation of lipolysis in adipocytes. In summary, the structural characteristics of respiratory microbiota in COPD and AECOPD patients are different from those in healthy populations, and their microbiota diversity decreases and microbial community structure and function will also undergo changes when acute exacerbations occur. In addition, the predicted microbial community function and metabolomics results indicate that the onset of AECOPD is mainly related to energy and amino acid metabolism disorders, especially alanine metabolism.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11526122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Bacillus velezensis</i> B105-8, a potential and efficient biocontrol agent in control of maize stalk rot caused by <i>Fusarium graminearum</i>.","authors":"Shuang Wang, Pinjiao Jin, Yanyan Zheng, Wang Kangkang, Jiaxin Chen, Jiansheng Liu, Yonggang Li","doi":"10.3389/fmicb.2024.1462992","DOIUrl":"10.3389/fmicb.2024.1462992","url":null,"abstract":"<p><strong>Introduction: </strong>Maize stalk rot (MSR), caused by <i>Fusarium graminearum</i>, is the most serious soil borne disease in maize production, seriously affecting maize yield and quality worldwide. Microbial biocontrol agents are the best means of controlling MSR and reducing the use of chemical fungicides, such as <i>Bacillus</i> spp.</p><p><strong>Methods and results: </strong>In the study, a soil-isolated strain B105-8 was identified as <i>B. velezensis</i> (accession No. PP325775.1 and No. PP869695.1), demonstrated a broad spectrum against various pathogens causing maize diseases, which effectively controlled MSR, exhibited a high control efficacy of more than 60% and growth-promoting effect in the pot plant. B105-8 could effectively improve soil urease (S-UE), invertase (S-SC), and catalase (S-CAT) activities. S-NP activity showed an initial increase with a peak of 20,337 nmol/h/g, followed by a decrease, but activity remained significantly better than control treatment with chemical fungicides. The application of B105-8 repaired the damage caused by <i>F. graminearum</i> on soil activity. The antifungal compound B-1, extracted from B105-8, was purified using a protein purifier, revealing inhibitory effects against <i>F. graminearum</i>. Mass spectrometry analysis indicated the potential presence of C14 Bacillomycin, C15 Iturin, C15 Mycosubtilin, C17, and C15 fengycin in B-1. In pot experiments, a 5 μL/mL concentration of B-1 exhibited 69% control on MSR, enhancing maize root elongation, elevation, and fresh weight. At 10 μL/mL, B-1 showed 89.0 and 82.1% inhibition on spore production and mycelial growth, causing hyphal deformities.</p><p><strong>Discussion: </strong>This study presents the innovative use of <i>B. velezensis</i>, isolated from maize rhizosphere in cold conditions to effectively control MSR caused by <i>F. graminearum</i>. The findings highlight the remarkable regional and adaptive characteristics of this strain, making it an excellent candidate to fight MSR in diverse environments. In conclusion, <i>B. velezensis</i> B105-8 demonstrated potential as a biocontrol agent for MSR.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current progress on the microbial therapies for acute liver failure.","authors":"Jiayuan Huang, Tianyu Xu, Guoqiao Quan, Yuange Li, Xiaoya Yang, Wenrui Xie","doi":"10.3389/fmicb.2024.1452663","DOIUrl":"10.3389/fmicb.2024.1452663","url":null,"abstract":"<p><p>Acute liver failure (ALF), associated with a clinical fatality rate exceeding 80%, is characterized by severe liver damage resulting from various factors in the absence of pre-existing liver disease. The role of microbiota in the progression of diverse liver diseases, including ALF, has been increasingly recognized, with the interactions between the microbiota and the host significantly influencing both disease onset and progression. Despite growing interest in the microbiological aspects of ALF, comprehensive reviews remain limited. This review critically examines the mechanisms and efficacy of microbiota-based treatments for ALF, focusing on their role in prevention, treatment, and prognosis over the past decade.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viral co-detection of influenza virus and other respiratory viruses in hospitalized Brazilian patients during the first three years of the coronavirus disease (COVID)-19 pandemic: an epidemiological profile.","authors":"Bianca Aparecida Siqueira, Ketlyn Oliveira Bredariol, Matheus Negri Boschiero, Fernando Augusto Lima Marson","doi":"10.3389/fmicb.2024.1462802","DOIUrl":"10.3389/fmicb.2024.1462802","url":null,"abstract":"<p><strong>Introduction: </strong>In Brazil, few studies were performed regarding the co-detection of respiratory viruses in hospitalized patients. In this way, the study aimed to describe the epidemiological profile of hospitalized patients due to influenza virus infection that presented co-detection with another respiratory virus.</p><p><strong>Methods: </strong>The epidemiological analysis was made by collecting data from Open-Data-SUS. The study comprised patients infected by the influenza A or B virus with positive co-detection of another respiratory virus, such as adenovirus, bocavirus, metapneumovirus, parainfluenza virus (types 1, 2, 3, and 4), rhinovirus, and respiratory syncytial virus (RSV). The markers [gender, age, clinical signs and symptoms, comorbidities, need for intensive care unit (ICU) treatment, and need for ventilatory support] were associated with the chance of death. The data was collected during the first three years of the coronavirus disease (COVID)-19 pandemic-from December 19, 2019, to April 06, 2023.</p><p><strong>Results: </strong>A total of 477 patients were included, among them, the influenza A virus was detected in 400 (83.9%) cases. The co-detection occurred, respectively, for RSV (53.0%), rhinovirus (14.0%), adenovirus (13.4%), parainfluenza virus type 1 (10.7%), parainfluenza virus type 3 (5.2%), metapneumovirus (3.8%), parainfluenza virus type 2 (3.6%), bocavirus (3.4%), and parainfluenza virus type 4 (1.5%). The co-detection rate was higher in the male sex (50.7%), age between 0-12 years of age (65.8%), and white individuals (61.8%). The most common clinical symptoms were cough (90.6%), dyspnea (78.8%), and fever (78.6%). A total of 167 (35.0%) people had at least one comorbidity, mainly cardiopathy (14.3%), asthma (8.4%), and diabetes mellitus (7.3%). The need for ICU treatment occurred in 147 (30.8%) cases, with most of them needing ventilatory support (66.8%), mainly non-invasive ones (57.2%). A total of 33 (6.9%) patients died and the main predictors of death were bocavirus infection (OR = 14.78 [95%CI = 2.84-76.98]), metapneumovirus infection (OR = 8.50 [95%CI = 1.86-38.78]), race (other races vs. white people) (OR = 3.67 [95%CI = 1.39-9.74]), cardiopathy (OR = 3.48 [95%CI = 1.13-10.71]), and need for ICU treatment (OR = 7.64 [95%CI = 2.44-23.92]).</p><p><strong>Conclusion: </strong>Co-detection between the influenza virus and other respiratory viruses occurred, mainly with RSV, rhinovirus, and adenovirus being more common in men, white people, and in the juvenile phase. Co-detection of influenza virus with bocavirus and metapneumovirus was associated with an increased chance of death. Other factors such as race, cardiopathy, and the need for an ICU were also associated with a higher chance of death.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of two commercial broth microdilution panels for multidrug-resistant Gram-negative bacteria: Thermo Scientific™ Sensititre DKMGN vs. Beckman Coulter MicroScan NMDRM1.","authors":"Antoine Aupaix, Kamila Lamraoui, Hector Rodriguez-Villalobos, Ahalieyah Anantharajah, Alexia Verroken","doi":"10.3389/fmicb.2024.1480687","DOIUrl":"10.3389/fmicb.2024.1480687","url":null,"abstract":"<p><strong>Introduction: </strong>Antimicrobial susceptibility testing (AST) using broth microdilution (BMD) is usually the reference method to obtain accurate minimum inhibitory concentrations and optimally manage infections with resistant organisms. Several commercial dry BMD are available for AST in clinical laboratories.</p><p><strong>Materials and methods: </strong>Two commercial BMD panels for testing of multidrug-resistant Gram-negative bacteria were compared: the Thermo Scientific™ Sensititre DKMGN and the Beckman Coulter NMDRM1, for 17 antimicrobial agents.</p><p><strong>Results: </strong>A total of 207 isolates were tested: three ATCC strains and one NCTC strain, six quality control strains from the Belgian National Antimicrobial Committee, and 197 clinical isolates, including carbapenem-resistant <i>Enterobacterales</i>, <i>Pseudomonas aeruginosa</i>, and <i>Acinetobacter baumannii</i>. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) 2023 breakpoints version 13.1 were used to assign susceptibility categories.</p><p><strong>Discussion: </strong>Overall, the categorical agreement (CA) and essential agreement (EA) were both above 90%, but several useful antibiotics for the treatment of multi-resistant organisms showed CA and EA under 90%, that is, meropenem, imipenem, and colistin for <i>Enterobacterales</i> and <i>meropenem</i> and colistin for <i>P. aeruginosa</i>. For <i>Enterobacterales</i>, the NMDRM1 panel showed a significantly higher resistance rate for meropenem, imipenem, amikacin, and colistin. For carbapenems, the minimal inhibitory concentrations (MICs) were underestimated by the DKMGN panel, as already pointed out by a warning on the EUCAST website. To better assess carbapenem susceptibility in carbapenem-resistant organisms, the DKMGN panel now requires the use of a higher inoculum in the insert kit. However, for a given isolate whose susceptibility to carbapenems is not known, there is a risk of underestimating the MIC values. Our results show that colistin testing remains a challenge, highlighting the urgent need for the development of more accurate commercial methods. The use of a single commercial method cannot guarantee good precision in the determination of the MIC value for colistin.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dependence of aerosol-borne influenza A virus infectivity on relative humidity and aerosol composition.","authors":"Ghislain Motos, Aline Schaub, Shannon C David, Laura Costa, Céline Terrettaz, Christos Kaltsonoudis, Irina Glas, Liviana K Klein, Nir Bluvshtein, Beiping Luo, Kalliopi Violaki, Marie O Pohl, Walter Hugentobler, Ulrich K Krieger, Spyros N Pandis, Silke Stertz, Thomas Peter, Tamar Kohn, Athanasios Nenes","doi":"10.3389/fmicb.2024.1484992","DOIUrl":"10.3389/fmicb.2024.1484992","url":null,"abstract":"<p><p>We describe a novel biosafety aerosol chamber equipped with state-of-the-art instrumentation for bubble-bursting aerosol generation, size distribution measurement, and condensation-growth collection to minimize sampling artifacts when measuring virus infectivity in aerosol particles. Using this facility, we investigated the effect of relative humidity (RH) in very clean air without trace gases (except ∼400 ppm CO₂) on the preservation of influenza A virus (IAV) infectivity in saline aerosol particles. We characterized infectivity in terms of 99%-inactivation time, <i>t</i> ₉₉, a metric we consider most relevant to airborne virus transmission. The viruses remained infectious for a long time, namely <i>t</i> ₉₉ > 5 h, if RH < 30% and the particles effloresced. Under intermediate conditions of humidity (40% < RH < 70%), the loss of infectivity was the most rapid (<i>t</i> ₉₉ ≈ 15-20 min, and up to <i>t</i> ₉₉ ≈ 35 min at 95% RH). This is more than an order of magnitude faster than suggested by many previous studies of aerosol-borne IAV, possibly due to the use of matrices containing organic molecules, such as proteins, with protective effects for the virus. We tested this hypothesis by adding sucrose to our aerosolization medium and, indeed, observed protection of IAV at intermediate RH (55%). Interestingly, the <i>t</i> ₉₉ of our measurements are also systematically lower than those in 1-μL droplet measurements of organic-free saline solutions, which cannot be explained by particle size effects alone.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Recent advancements in mycobacterial diseases research.","authors":"Matt D Johansen, Jotam G Pasipanodya, Selvakumar Subbian, Vishwanath Venketaraman","doi":"10.3389/fmicb.2024.1504449","DOIUrl":"10.3389/fmicb.2024.1504449","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Isolation of <i>Limosilactobacillus mucosae</i> G01 with inhibitory effects on porcine epidemic diarrhea virus <i>in vitro</i> from Bama pig gastroenteritis.","authors":"Bin Zhang, Haiyan Shen, Hongchao Gou, Nile Wuri, Chunhong Zhang, Zhicheng Liu, Haiyan He, Jingjing Nie, Yunzhi Qu, Letu Geri, Jianfeng Zhang","doi":"10.3389/fmicb.2024.1488274","DOIUrl":"10.3389/fmicb.2024.1488274","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fmicb.2024.1360098.].</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11526114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into the spatial variability of microbial diversity and density in peatlands exposed to various electron acceptors with an emphasis on methanogenesis and CO₂ fluxes.","authors":"Sadaf Shabbir, Chang Qian, Muhammad Faheem, Fengwu Zhou, Zhi-Guo Yu","doi":"10.3389/fmicb.2024.1468344","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1468344","url":null,"abstract":"<p><p>Peatlands are vital in the global carbon cycle, acting as significant sinks for carbon and releasing methane (CH₄) and carbon dioxide (CO₂) into the atmosphere. However, the complex interactions between environmental factors and the microbial communities responsible for these greenhouse gas emissions remain insufficiently understood. To address this knowledge gap, a pilot-scale mesocosm study was conducted to assess the impact of different terminal electron acceptors (TEAs), including sulfate (SO₄ ^2-), humic acid (HA), and goethite, on CH₄ and CO₂ emissions and microbial community structures in peatlands. Our results revealed that the addition of TEAs significantly altered the CH₄ and CO₂ emissions. Specifically, the addition of SO₄ ^2- nearly doubled CO₂ production while substantially inhibiting CH₄ emissions. The combined addition of SO₄ ^2- and HA, as well as HA alone, followed a similar pattern, albeit with less pronounced effects on CH₄. Goethite addition resulted in the highest inhibition of CH₄ among all treatments but did not significantly increase CO₂ production. Community composition and network analysis indicated that TEAs primarily determined the structure of microbial communities, with each treatment exhibiting distinct taxa networks. <i>Proteobacteria</i>, <i>Acidobacteria</i>, <i>Chloroflexi,</i> and <i>Bacteroidetes</i> were the most abundant phyla across all mesocosms. The presence of methanotrophs, including <i>Methylomirabilales</i> and <i>Methylococcales</i>, was linked to the inhibition of CH₄ emissions in these mesocosms. This study provides novel insights into the spatial variability of microbial diversity and density in peatlands under various TEAs, emphasizing the role of methanogenesis and CO₂ fluxes in carbon cycling. Our findings enhance the understanding of carbon cycling in microbe-rich environments exposed to TEAs and highlight the potential for future studies to investigate the long-term effects of TEAs on microbial communities, enzymes, and carbon storage.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11520324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and synthesis of quorum-sensing agonist for improving biofilm formation and the application of <i>Acidithiobacillus thiooxidans</i> in bioleaching.","authors":"Deping Tang, Yanpeng Xi, Wentao Song, Mengjiao Li, Yali Liu, Yanyan Lin, Ran Zhang, Aihong Mao","doi":"10.3389/fmicb.2024.1465633","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1465633","url":null,"abstract":"<p><strong>Introduction: </strong>Currently, there are few investigations on the effect of a synthetic exogenous quorum sensing (QS) agonist on the bioleaching rate of <i>Acidithiobacillus thiooxidans</i> (<i>A. thiooxidans</i>).</p><p><strong>Methods: </strong>We created AHL (N-acyl-homoserine lactone) analogues and investigated their effects on <i>A. thiooxidans</i> biofilm formation, adsorption kinetics, bioleaching, and mechanism.</p><p><strong>Results: </strong>The findings revealed that N-(3-thiolactone)- dodecylamine (Y3) significantly increased the biofilm formation of <i>A. thiooxidans</i> in 96-well plates and sulfur sheets. Adsorption tests revealed that Y3 increased the adhesion rate, adsorption constant, and adsorption efficiency. Bioleaching tests indicated that Y3 boosted bioleaching efficiency, with Ni²⁺ and Cu²⁺ bioleaching rates increasing by 49.13% and 33.03%, respectively. Transcriptomic analysis revealed that Y3 increased genes associated with QS pathways and biofilm formation, particularly <i>afeI</i>, which was dramatically elevated 42 times.</p><p><strong>Discussion: </strong>The study laid the groundwork for a better understanding of the mechanics of <i>A. thiooxidans</i> biofilm formation, which could help improve the potential application of <i>A. thiooxidans</i> in bioleaching.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}