Frontiers in Microbiology最新文献

{"title":"Transcriptomic and metabolomic analyses reveal the spatial role of carnitine metabolism in the progression of hepatitis B virus cirrhosis to hepatocellular carcinoma.","authors":"Pengxiang Gao, Qiuping Liu, Ziye Luo, Wenjun Pu","doi":"10.3389/fmicb.2024.1461456","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1461456","url":null,"abstract":"<p><strong>Introduction: </strong>Liver cirrhosis (LC) and hepatocellular carcinoma (HCC) resulting from chronic hepatitis B virus (HBV) infection are major health concerns. Identifying critical biomarkers and molecular targets is needed for early diagnosis, prognosis, and therapy of these diseases.</p><p><strong>Methods: </strong>In this study, we explored the gene expression and metabolism in the liver tissues of LC, HCC, and healthy controls, to analyse and identify potential biomarkers of disease progression. Mass spectrometry imaging was used to evaluate the spatial distribution of key metabolites.</p><p><strong>Results and discussion: </strong>The results revealed significant changes in gene expression and metabolic pathways along with disease progression. The upregulated genes were associated with extracellular matrix remodeling and cancer pathways, including LAMC1-3, COL9A2, COL1A1, MYL9, MYH11, and KAT2A. The downregulated genes were linked to immune response and fatty acid metabolism. Metabolomic analysis showed major changes in lipid and choline metabolism. Consistent changes in the expression of specific genes and metabolites were correlated with clinical data. Notably, metabolites such as L-acetylcarnitine, histamine, and 4-trimethylammoniobutanoic acid demonstrated high accuracy (AUC > 0.85) in distinguishing between healthy, LC, and HCC groups. This study identifies key gene and metabolite changes in HBV related LC and HCC, highlighting critical pathways involved in disease progression. Biomarkers like L-acetylcarnitine and KAT2A show promise for early diagnosis and prognosis, potentially improving outcomes for hepatitis liver disease patients.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1461456"},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reviewing advancement in <i>Mycoplasma pneumoniae</i> P30 adhesin protein provides insights for future diagnosis and treatment.","authors":"Yingying Zuo, Ru Zhang, Shuihong Li","doi":"10.3389/fmicb.2024.1515291","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1515291","url":null,"abstract":"<p><p><i>Mycoplasma pneumoniae</i> is a major pathogen that causes upper and lower respiratory tract infections in children, adolescents, and elderly individuals and can lead to pneumonia, intrapulmonary and extrapulmonary complications, and respiratory sequelae. <i>M. pneumoniae</i> must adhere to respiratory epithelial cells of a host for infection. The P1 and P30 proteins, as two adhesin proteins of <i>M. pneumoniae</i>, have attracted extensive attention from many researchers. In this paper, we present the latest research progress on the P30 protein in terms of structure and mutation typing, physiological function, clinical serological diagnosis and vaccine development in a literature review. This study deepens our knowledge on the pathogenesis of <i>M. pneumoniae</i> and is useful for diagnosing and preventing <i>M. pneumoniae</i> infection.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1515291"},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation of high-quality mixed silage from paper mulberry and wheat bran driven by the characteristics of the microbial community.","authors":"Wenbo Wang, Hua Tian, Yuwei Zhao, Yanshun Nie, Zibing Li, Junjie Gong, Wenjie Jiang, Yanjing Yin, Ramon Santos Bermudez, Wenxing He","doi":"10.3389/fmicb.2024.1476067","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1476067","url":null,"abstract":"<p><p>Paper mulberry (<i>Broussonetia papyrifera</i>) is a high-quality silage protein feed material that can help address feed shortages and support livestock development. Although some studies have investigated the relationships between microbial communities and silage quality, these relationships and the underlying community assembly processes remain complex, requiring further research to clarify them. Additionally, limited research has explored the relationship between microbial community fermentation functions and silage quality. In this study, we aimed to explore <i>B. papyrifera</i> and wheat bran mixed silage quality driven by the characteristics of the microbial community. After 50 days of silage fermentation, high-quality and low-quality samples were selected from every mixing ratio (90:10, 80:20, and 65:35). The silage chemical composition, lignocellulose degradation enzyme activity, microbial community composition, and potential functions were used to explore the relevance between silage quality and the characteristics of the microbial community. The contents of hemicellulose, neutral detergent fiber, pH, and the activities of endoglucanase and exoglucanase were significantly affected by mixing ratios and silage quality grade. There were higher crude protein content, lignocellulose degrading enzyme activity, and lower pH, lignin, and acid detergent fiber in the mixing of 65:35 (BP65%) samples. The PERMANOVA results showed that mixing ratios had significant impacts on microbial community composition and bacterial fermentation functions. There was a higher bacterial diversity, lower fungal diversity, and better functional potentials for fermentation and lignocellulose degradation in BP65% high-quality silage. The dominant genera were <i>Lactobacillus</i>, <i>Cladosporium</i>, and <i>Wallemia</i> in all samples. The relative abundance of <i>Clostridium</i>, <i>Rhodococcus</i>, <i>Turicibacter</i>, <i>Ralstonia</i>, and <i>Burkholderia</i> was significantly higher in BP65% high-quality samples. There was a higher abundance of <i>Wallemia</i> in the BP65% samples than in other mixing ratios samples. Notably, silage quality showed a close relationship with <i>Lactobacillus</i>, <i>Turicibacter</i>, <i>Romboutsia</i>, <i>Wallemia,</i> and <i>Pichia</i>. In summary, 65:35 was a suitable mixing ratio for <i>B. papyrifera</i> and wheat bran silage, but high-quality silage still required the participation of multiple specific rare microbial taxa. The higher bacterial diversity and specific microbial taxa abundance could be critical for improving <i>B. papyrifera</i> silage quality. We expect that our findings will provide new insights into silage quality driven by the characteristics of the microbial community.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1476067"},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A bifunctional endolytic alginate lyase with two different lyase catalytic domains from <i>Vibrio</i> sp. H204.","authors":"Chune Peng, Qingbin Wang, Wei Xu, Xinkun Wang, Qianqian Zheng, Xiaohui Liang, Xiaodan Dong, Fuchuan Li, Lizeng Peng","doi":"10.3389/fmicb.2024.1509599","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1509599","url":null,"abstract":"<p><p>Alginate lyases can fully degrade alginate into various size-defined unsaturated oligosaccharide products by <i>β</i>-elimination. Here, we identified the bifunctional endolytic alginate lyase Aly35 from the marine bacterium <i>Vibrio</i> sp. Strain H204. The enzyme Aly35 is classified into the polysaccharide lyase 7 superfamily and contains two alginate lyase catalytic domains. The relationship and function of the two lyase domains are not well known. Thus, the full-length recombinant enzyme and its truncated proteins Aly35-CD1 (catalytic domain 1), Aly35-CD2 (catalytic domain 2 domain) were constructed. The three enzymes showed similar biochemical characteristics and exhibited temperature and pH stability. Further research showed that Aly35 and Aly35-CD2 can efficiently degrade alginate, polymannuronate (PM) and polyguluronate (PG) into a series of unsaturated oligosaccharides, while Aly35-CD1 exhibits greater PM-degrading activity than that of Aly35-CD2 but can not degraded PG efficiently. The results suggest that the domain (Trp²⁹⁵-His⁵⁸²) is critical for PG-degrading activity, the domain has (Leu⁵³-Lys²⁸⁶) higher PM-degrading activity, both catalytic domains together confer increased alginate (including M-blocks and G blocks)-degrading activity. The enzyme Aly35 and its truncations Aly35-CD1 and Aly35-CD2 will be useful tools for structural analyses and for preparing bioactive oligosaccharides, especially Aly35-CD1 can be used to prepare G unit-rich oligosaccharides from alginate.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1509599"},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid, sensitive, and visual detection of mandarin fish ranavirus and infectious spleen and kidney necrosis virus using an RPA-CRISPR/Cas12a system.","authors":"Zhoutao Lu, Mincong Liang, Chuanrui Li, Yichun Xu, Shaoping Weng, Jianguo He, Changjun Guo","doi":"10.3389/fmicb.2024.1495777","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1495777","url":null,"abstract":"<p><p>Iridoviruses are large cytoplasmic icosahedral viruses that contain dsDNA. Among them, mandarin fish ranavirus (MRV) and infectious spleen and kidney necrosis virus (ISKNV) are particularly notable due to their high contagiousness and pathogenicity. These viruses pose a significant threat to fish aquaculture, resulting in substantial annual economic losses for the fish farming industry. Therefore, the development of novel, rapid virus detection technologies is essential for the prevention and control of ISKNV and MRV diseases. In this study, we developed a rapid, sensitive, and visual detection method for MRV and ISKNV using the recombinase polymerase amplification (RPA)-CRISPR/Cas12a system. This method can detect as low as 1 copy/μL of MRV and 0.1 copy/μL of ISKNV, demonstrating excellent specificity and reproducibility. The detection can be performed at a constant temperature of 37-39°C, eliminating the need for complex equipment. A 30-min RPA amplification followed by a 15-min CRISPR/Cas reaction is sufficient for detecting most samples. For low-concentration samples, extending the CRISPR/Cas reaction time to 60 min improves result visibility. The designed RPA reaction system is capable of performing reverse transcription of RNA, allowing for the detection of mRNA transcribed from the MCP gene of MRV and ISKNV in the sample. Furthermore, two probes were identified that can be observed without the need for excitation light. In conclusion, a field-suitable detection method for ISKNV and MRV has been established, providing a powerful tool for the prompt diagnosis of these aquatic pathogens and aiding in the prevention and control of ISKNV and MRV diseases.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1495777"},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and bioefficacy of grapevine bacterial endophytes against <i>Colletotrichum gloeosporioides</i> causing anthracnose disease.","authors":"Somnath K Holkar, Vrushali C Bhanbhane, Prabhavati S Ghotgalkar, Harshavardhan N Markad, Tushar D Lodha, Sujoy Saha, Kaushik Banerjee","doi":"10.3389/fmicb.2024.1502788","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1502788","url":null,"abstract":"<p><strong>Introduction: </strong>Grapevine (<i>Vitis vinifera</i> L.), one of the economically important fruit crops cultivated worldwide, harbours diverse endophytic bacteria (EBs) responsible for managing various fungal diseases. Anthracnose (<i>Colletotrichum gloeosporioides</i>) (Penz.) is one of the major constraints in quality grape production and therefore its management is a major concern among the grape growers.</p><p><strong>Materials and methods: </strong>Among the 50 EBs isolated from healthy leaf segments from the eight grapevine genotypes, biologically potential 20 EBs were purified and identified based on morphological, and biological characteristics and sequence analysis of 16S rRNA region. The antagonistic activities of EBs against <i>Colletotrichum gloeosporioides</i> were studied <i>in vitro</i> conditions.</p><p><strong>Results: </strong>The colony morphologies of EBs are white and yellow-coloured colonies, circular to irregular in shape, and entire, and flat margins. Among the 20 purified EBs, 19 isolates were found to be Gram-positive except one i.e., MS2 isolate. The 12 isolates reduced nitrate and 14 isolates produced urease enzyme. The <i>in vitro</i> assay revealed that two isolates, SB4 and RF1, inhibited 56.1% and 55.6% mycelial growth of <i>C. gloeosporioides</i>, respectively. Further, the identity of EBs was confirmed through PCR amplification of the 16S rRNA region resulting in ~1400 bp size amplicons. The sequence analysis of representative 15 isolates revealed that 5 EB isolates viz., SB5, CS2, RG1, RF1, C1 were identified as <i>Bacillus subtilis</i> with >99% sequence identity, two EBs viz., SB3, and CS1 were identified as <i>B. subtilis</i> subsp. <i>subtilis</i>, two EBs viz., SB1, and CS4 were identified as <i>B. licheniformis</i>. The SB2 isolate was identified as <i>Bacillus</i> sp., whereas SB4 as <i>Brevibacillus borstelensis</i>, TH1 as <i>B. velezensis</i>, TH2 as <i>B. tequilensis</i>, CS3 as <i>B. pumilus</i> and MS1 as <i>Micrococcus luteus</i> were identified.</p><p><strong>Conclusion: </strong>The phylogenetic analysis of 16S rRNA sequence revealed eight distinct clades and showed the close clustering of identified species with the reference species retrieved from NCBI GenBank. The current investigation provides the scope for further field evaluations of these endophytic microbes for managing anthracnose disease.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1502788"},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11684392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing lactic acid bacteria for nicotinamide mononucleotide biosynthesis: a review of strategies and future directions.","authors":"Linghui Kong, Xinyu Li, Taiyu Liu, Qingshou Yao, Jiayang Qin","doi":"10.3389/fmicb.2024.1492179","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1492179","url":null,"abstract":"<p><p>Nicotinamide mononucleotide (NMN), one of the crucial precursors of nicotinamide adenine dinucleotide, has garnered considerable interest for its pharmacological and anti-aging effects, conferring potential health and economic benefits for humans. Lactic acid bacteria (LAB) are one of the most important probiotics, which is commonly used in the dairy industry. Due to its probiotic properties, it presents an attractive platform for food-grade NMN production. LAB have also been extensively utilized to enhance the functional properties of pharmaceuticals and cosmetics, making them promising candidates for large-scale up synthesis of NMN. This review provides an in-depth analysis of various metabolic engineering strategies, including enzyme optimization, pathway rewiring, and fermentation process enhancements, to increase NMN yields in LAB. It explores both CRISPR/Cas9 and traditional methods to manipulate key biosynthetic pathways. In particular, this study discussed future research directions, emphasizing the application of synthetic biology, systems biology, and AI-driven optimization to further enhance NMN production. It provides invaluable insights into developing scalable and industrially relevant processes for NMN production to meet the growing market demand.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1492179"},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11681623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in research on the impact and mechanisms of pathogenic microorganism infections on pyroptosis.","authors":"Pan Shang, Mailin Gan, Ziang Wei, Shijie Hu, Lei Song, Jinkang Feng, Lei Chen, Lili Niu, Yan Wang, Shunhua Zhang, Linyuan Shen, Li Zhu, Ye Zhao","doi":"10.3389/fmicb.2024.1503130","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1503130","url":null,"abstract":"<p><p>Pyroptosis, also known as inflammatory necrosis, is a form of programmed cell death characterized by the activation of gasdermin proteins, leading to the formation of pores in the cell membrane, continuous cell swelling, and eventual membrane rupture. This process results in the release of intracellular contents, including pro-inflammatory cytokines like IL-1β and IL-18, which subsequently trigger a robust inflammatory response. This process is a crucial component of the body's innate immune response and plays a significant role in combating infections. There are four main pathways through which pathogenic microorganisms induce pyroptosis: the canonical inflammasome pathway, the non-canonical inflammasome pathway, the apoptosis-associated caspase-mediated pathway, and the granzyme-mediated pathway. This article provides a brief overview of the effects and mechanisms of pathogen infections on pyroptosis.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1503130"},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of virus-rich intermediate cells as crucial players in SARS-CoV-2 infection and differentiation dynamics of human airway epithelium.","authors":"Mi Il Kim, Choongho Lee","doi":"10.3389/fmicb.2024.1507852","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1507852","url":null,"abstract":"<p><p>Understanding the early interactions between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and human airway epithelial cells is essential for unraveling viral replication and spread mechanisms. In this study, we investigated the early dynamics of airway epithelial cells during SARS-CoV-2 infection using well-differentiated human nasal and tracheal epithelial cell cultures by incorporating three publicly available single-cell RNA sequencing datasets. We identified a previously uncharacterized cell population, termed virus-rich intermediate (VRI) cells, representing an intermediate differentiation stage between basal and ciliated cells. These VRI cells exhibited high viral loads at all infection time points, strong interferon and inflammatory responses, increased mRNA expression of microvilli-related genes (PAK1, PAK4, VIL1), and suppression of apoptosis markers (BAX, CASP3) alongside increased anti-apoptotic gene expression (BCL2). Cell-cell interaction analysis revealed that VRI cells send signals to basal cells via receptor-ligand pathways such as EPHA and VEGF, likely promoting basal cell differentiation and proliferation through MAPK signaling. These findings suggest that SARS-CoV-2 utilizes VRI cells as a primary site for replication and spread, leveraging these cells' unique differentiation state to evade host cell death and facilitate viral propagation. This study provides insights into the early cellular responses to SARS-CoV-2 infection and highlights potential therapeutic targets to limit viral spread.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1507852"},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11681626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and functional traits based indigenous rhizosphere associated phosphate solubilizing bacteria for sustainable production of rice.","authors":"Maria Rasul, Mahreen Yahya, Muhammad Suleman, Sughra Hakim, Babur S Mirza, Muhammad Sajjad Mirza, Thomas Reitz, Mika Tapio Tarkka, Sumera Yasmin","doi":"10.3389/fmicb.2024.1470019","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1470019","url":null,"abstract":"<p><strong>Introduction: </strong>Rice, particularly Basmati rice, holds significant global importance as a staple food. The indiscriminate use of phosphate-based fertilizers during rice production has led to high residual levels of these chemicals in soil, impacting soil health and fertility. This study aimed to address this challenge by investigating the potential of phosphate solubilizing bacteria (PSB) in improving soil fertility and boosting the growth of Basmati rice.</p><p><strong>Methods: </strong>Using amplicon-based 16S rDNA sequencing, bacterial isolation and cultivation, conducting greenhouse and field experiments, and PSB localization, we optimized the search for PSB inoculants to enhance Basmati rice growth.</p><p><strong>Results and discussion: </strong>Rice rhizosphere prokaryote communities showed significant differences in microbial diversity and composition between between basmati and non-basmati rice cultivated areas. Dominant bacterial phyla included Proteobacteria, Acidobacteria, Actinobacteria, and Firmicutes, with Actinobacteria and Proteobacteria playing a crucial role in nutrient recycling. Isolation and optimization of PSB strains, including Acinetobacter sp. MR5 and Pseudomonas sp. R7, were carried out and soil microcosm studies confirmed their efficacy in increasing soil available phosphorus concentration. Response surface methodology revealed the relative importance of factors such as pH, inoculum density and incubation temperature in maximising phosphate solubilization. Microplot experiments demonstrated the effectiveness of optimized PSB inoculants in promoting Basmati rice growth, with significant increases in plant height, tiller number, biomass, and grain yield compared to uninoculated controls. A consortium of PSB proved superior to single-strain inoculants, even with reduced chemical fertilizer application. Field trials at several rice growing sites confirmed the positive impact of the PSB consortium on grain yield, soil phosphorus availability, and plant phosphorus uptake. The competence and persistence of the inoculated strains in the rhizosphere was confirmed by FISH and BOX Polymerase Chain Reaction (BOX-PCR). This work highlights the potential of PSB-based biofertilizers to improve soil fertility, promote sustainable rice production and reduce the negative environmental impacts of chemical fertilizers. Future research would focus on scaling up these findings for widespread adoption in agriculture and exploring their applicability to other crops and agroecosystems.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1470019"},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}