Frontiers in Microbiology最新文献

{"title":"Cohort analysis of high-risk HPV infection in adult women in Dapeng New District, Shenzhen, Guangdong Province, China.","authors":"Weifeng Wei, Mi Zhang, Yiyuan Lin, Ziyin Li, Wenbo Luo, Weihua Zhao, Jing Zhuang, Weining Zhao, Zhixue Xu, Gaomin Li, Wenjing Zeng, Liping Huang, Yan Tan, Zhiying Yu, Guanglei Li","doi":"10.3389/fmicb.2025.1539209","DOIUrl":"10.3389/fmicb.2025.1539209","url":null,"abstract":"<p><strong>Introduction: </strong>Cervical cancer is the fourth most commonly diagnosed malignancy among women globally, with HPV infection being the primary cause. Large-scale HPV screening is crucial for early detection, as appropriate intervention in HPV-positive individuals can significantly reduce cervical cancer incidence and mortality.</p><p><strong>Methods: </strong>Data were extracted from the Shenzhen Maternal and Child Health Management Information System, covering 18,667 HPV infection and cervical cancer screening instances among 15,850 women from January 1, 2020, to December 31, 2023. HPV prevalence and corresponding 95% confidence intervals (CIs) were calculated using SPSS (version 27.0).</p><p><strong>Results: </strong>From January 2020 to December 2023, the overall HPV infection rate was 12.39%, with a notably higher rate of 23.38% in women over 60 years. The hr-HPV infection rate was 12.99%, with HPV types 52, 58, 16, 51, and 68 being the most common. Among women with positive ThinPrep Cytologic Test (TCT) results, the hr-HPV infection rate was 53.37%. In women with positive cervical histopathology, the hr-HPV infection rate reached 95.95%. With increased HPV vaccination doses, the infection rates of HPV16, 18, 52, 58, 51, 31, 33, 45, 6, and 11 decreased among individuals under 30, but increased in those over 45.</p><p><strong>Discussion: </strong>These findings suggest that enhancing HPV vaccination coverage and cervical cancer screening, especially for women over 45, are effective strategies to reduce hr-HPV infection rates and cervical cancer incidence in this region.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1539209"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unique microbial communities in ancient volcanic ash layers within deep marine sediments are structured by the composition of iron phases.","authors":"Sönke Rolfes, Jack Longman, Katharina Pahnke, Bert Engelen","doi":"10.3389/fmicb.2025.1526969","DOIUrl":"10.3389/fmicb.2025.1526969","url":null,"abstract":"<p><p>Much of the marine sedimentary environment is affected by the deposition of tephra, the explosive products of volcanic eruptions. These tephra layers' geochemical and physical properties often differ substantially from those of the surrounding sediment, forming an extreme carbon-lean environment within the anoxic deep biosphere. Despite this, evidence suggests tephra layers harbor diverse and abundant microbial communities. While little is known about the composition of these communities and even less about their life modes, there is evidence indicating that iron (Fe) plays a vital role for these microorganisms. Here, we aim to link differences in the iron content of tephra layers and surrounding sediments with changes within microbial communities. We combined next-generation sequencing of 16S rRNA genes with geochemical analyses of Fe phases preserved in ancient tephra and sediments recovered from the Norwegian Margin during Expedition 396 of the International Ocean Discovery Program (IODP). In these samples, basaltic tephra contained nearly double Fe_total as surrounding sediments, with the majority hosted in \"reducible\" Fe(III) oxides, whilst sedimentary Fe is primarily in \"easily reducible\" Fe(III) oxides. Basaltic tephra harbored distinct microbial communities that differed from the surrounding sediment in composition and predicted metabolic properties. These predictions suggest a higher potential for the assimilatory use of more complex Fe(III) sources in tephra, indicating the microbes are able to exploit the \"reducible\" Fe(III) found in high quantities in these layers. Our findings confirm the few previous studies that have suggested distinct microbial communities to occur in marine tephra layers. Deciphering the role of iron for indigenous microorganisms hints at how life might flourish in this extreme environment. This has implications for understanding tephra layers as a ubiquitous component of the deep biosphere.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1526969"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Global dissemination and evolution of epidemic multidrug-resistant Gram-negative bacterial pathogens: surveillance, diagnosis and treatment, volume III.","authors":"João Pedro Rueda Furlan, Raffaele Zarrilli, Ziad Daoud, Fang He, Zhi Ruan","doi":"10.3389/fmicb.2025.1583112","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1583112","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1583112"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Probiotic effects on skin health: comprehensive visual analysis and perspectives.","authors":"Kexin Deng, Xiaofei Fan, Zhigen Yuan, Dian Li","doi":"10.3389/fmicb.2025.1583612","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1583612","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fmicb.2024.1453755.].</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1583612"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis on the regulatory mechanism of active ingredient accumulation during fermentation process of Massa Medicata Fermentata: microbe and metabolic profiles.","authors":"Yun Li, Ya-Juan Wang, Xiao-Peng Guo, Hong-Yuan Zhao, Hai-Wei Ren, Hong-Yu Li","doi":"10.3389/fmicb.2025.1548427","DOIUrl":"10.3389/fmicb.2025.1548427","url":null,"abstract":"<p><strong>Background: </strong>Massa Medicata Fermentata (MMF) is a traditional medicinal/edible fermented product; however, comprehensive research on the fermentation process from a microscopic perspective remains limited. In this study, we aimed to investigate the dynamic changes and correlations of physicochemical properties, microbial communities, and metabolite profiles in different fermentation stages (0, 48, 72, and 96 h) of MMF.</p><p><strong>Methods: </strong>Standard analytical tests, microbiome sequencing, broad-target metabolism, mixed standard-based mass spectrometry, and fine structure analysis were integrated to elucidate fluctuations in physicochemical, microbial, and metabolic levels during MMF fermentation.</p><p><strong>Results: </strong>During the fermentation process, bacterial diversity generally shows an increasing trend, whereas fungal diversity generally shows a decreasing trend. Revealing that the differentially abundant metabolites were primarily categorized into lipids, amino acids and derivatives, phenolic acids, organic acids, flavonoids, lignans and coumarins, nucleotides and derivatives, and alkaloids. Structural equation modeling and correlation analysis indicated that two species of bacteria (<i>Bacillus velezensis</i>, <i>Bacillus safensis</i>) and four species of fungi (<i>Apiotrichum montevideense</i>, <i>Geotrichum bryndzae</i>, f_Dipodascaceae, <i>Saccharomycopsis fibuligera</i>) showed significant positive correlations with five types of differential metabolites, including lipids, flavonoids, phenolic acids, lignans and coumarins, and organic acids. These differential metabolites are essential components responsible for the therapeutic effects of MMF, particularly those that reach peak concentrations at 72 h of fermentation.</p><p><strong>Conclusion: </strong>These findings are expected to provide a reference for developing strategies to strengthen the quality of MMF and promote its modern application.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1548427"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of <i>Helicobacter pylori</i> eradication on gastric cancer risk in patients with intestinal metaplasia or dysplasia: a meta-analysis of randomized controlled trials.","authors":"Qiang Fu, Huidong Yu, Ming Liu, Liang Chen, Weiyang Chen, Ziyi Wang, Wenya Li","doi":"10.3389/fmicb.2025.1530549","DOIUrl":"10.3389/fmicb.2025.1530549","url":null,"abstract":"<p><strong>Background: </strong>Observational studies suggest that <i>Helicobacter pylori</i> (<i>H. pylori</i>) is associated with an increased risk of gastric cancer, yet the effect of <i>H. pylori</i> eradication on gastric cancer risk in patients with intestinal metaplasia (IM) or dysplasia remains controversial. The purpose of this study was to summarize the evidence from randomized controlled trials (RCTs) investigating <i>H. pylori</i> eradication on gastric cancer risk in patients with IM or dysplasia to determine the evidence base.</p><p><strong>Methods: </strong>PubMed, Embase, Cochrane Library, Web of science and China National Knowledge Internet database were searched for RCTs published through May 2024 in adults with IM or dysplasia comparing the risk of gastric cancer following <i>H. pylori</i> eradication versus no eradication therapy. Relative risk (RR) with its 95% confidence interval (CI) using random-effects model were employed for the effect estimate. Sensitivity, meta-regression, and subgroup analyses were also calculated.</p><p><strong>Results: </strong>Sixteen RCTs involving 15,027 patients with IM or dysplasia met the inclusion criteria. In a pooled analysis, <i>H. pylori</i> eradication resulted in a 45% reduction in RR for gastric cancer risk relative to no eradication (RR: 0.55; 95% CI: 0.46-0.67; <i>p</i> < 0.001). <i>H. pylori</i> eradication significantly reduced the risk of gastric cancer in patients with dysplasia (RR: 0.51; 95% CI: 0.32-0.82; <i>p</i> = 0.005), and IM (RR: 0.61; 95% CI: 0.40-0.93; <i>p</i> = 0.022). Further, if the study conducted in countries other than those in Asia, sample size <500, percentage of male <50.0%, follow-up duration <5.0 years, and low study quality, then there was no significant association between <i>H. pylori</i> eradication and a decreased risk of gastric cancer.</p><p><strong>Conclusion: </strong><i>H. pylori</i> eradication is protective against gastric cancer in patients with IM or dysplasia.</p><p><strong>Systematic review registration: </strong>INPLASY202530010, https://inplasy.com/.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1530549"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspectives on the microorganisms with the potentials of PET-degradation.","authors":"Xiao-Huan Liu, Jun-Li Jin, Hai-Tong Sun, Shuo Li, Fei-Fei Zhang, Xin-Hong Yu, Qi-Zhi Cao, Yu-Xuan Song, Nan Li, Zhen-Hua Lu, Tao Wang, Fei Liu, Jian-Min Wang","doi":"10.3389/fmicb.2025.1541913","DOIUrl":"10.3389/fmicb.2025.1541913","url":null,"abstract":"<p><p>Polyethylene terephthalate (PET), a widely used synthetic polymer in daily life, has become a major source of post-consumer waste due to its complex molecular structure and resistance to natural degradation, which has posed a significant threat to the global ecological environment and human health. Current PET-processing methods include physical, chemical, and biological approaches, however each have their limitations. Given that numerous microbial strains exhibit a remarkable capacity to degrade plastic materials, microbial degradation of PET has emerged as a highly promising alternative. This approach not only offers the possibility of converting waste into valuable resources but also contributes to the advancement of a circular economy. Therefore in this review, it is mainly focused on the cutting-edge microbial technologies and the key role of specific microbial strains such as <i>Ideonella sakaiensis</i> 201-F6, which can efficiently degrade and assimilate PET. Particularly noteworthy are the catalytic enzymes related to the metabolism of PET, which have been emphasized as a sustainable and eco-friendly strategy for plastic recycling within the framework of a circular economy. Furthermore, the study also elucidates the innovative utilization of degraded plastic materials as feedstock for the production of high-value chemicals, highlighting a sustainable path forward in the management of plastic waste.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1541913"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surfactin: a novel <i>Aphis gossypii</i> killing surfactin produced by <i>Bacillus australimaris</i> TRM82479 of Taklamakan Desert origin.","authors":"Yelin Wang, Zhibin Sun, Shiyu Wang, Feng Wen, ZhanFeng Xia","doi":"10.3389/fmicb.2025.1559495","DOIUrl":"10.3389/fmicb.2025.1559495","url":null,"abstract":"<p><strong>Introduction: </strong>The cotton aphid <i>Aphis gossypii</i> poses a global, serious threat to cotton yield and quality. Although chemical pesticides are effective, pollution and resistance are increasingly prominent, making development of new biopesticides a priority in the context of green agricultural development.</p><p><strong>Methods: </strong>Given that reports on the activity of surfactins against <i>A. gossypii</i> are limited, here, 107 <i>Bacillus</i> strains isolated from the extreme environment of the Chinese Taklamakan Desert were screened for insecticidal activity against <i>A. gossypii</i> using the leaf-dip method. Active strains were characterized by morphological observation, 16S rRNA gene sequencing, and phylogenetic analysis. Secondary metabolite synthesis genes were identified by whole-genome sequencing and antiSMASH analysis.</p><p><strong>Results: </strong><i>B. australimaris</i> strain TRM82479 showed 75.00% 48-h mortality against <i>A. gossypii</i>. An antiSMASH analysis showed that this strain contains several gene clusters related to the synthesis of nonribosomal peptide (NRP) fengycin and lichenysin lipopeptide analogs. Cluster 1 has the highest similarity of 52% with the fengycin synthesis gene cluster, and Cluster 8 has the highest similarity of 92% with the lichenysin synthesis gene cluster. It is inferred that <i>B. australimaris</i> strain can produce lipopeptide analogs distinct from fengycin and lichenysin, so we isolated and identified its NRPs. The results showed that surfactin is the main insecticidal substance, with an LC₅₀ of 0.857 mg/mL and an LC₉₅ of 4.350 mg/mL against cotton aphids in aqueous solution. The results of the zebrafish acute toxicity experiment showed that surfactins are low-toxic to fish, indicating good biological safety.</p><p><strong>Discussion: </strong>This study not only provides new strain resources for cotton aphid control but also demonstrates the potential of surfactins as biopesticides, laying a foundation for their future agricultural application.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1559495"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences and correlation analysis of feeding habits and intestinal microbiome in <i>Schizopygopsis microcephalus</i> and <i>Ptychobarbus kaznakovi</i> in the upper reaches of Yangtze River.","authors":"Xinyu Wang, Jiahui Hao, Cunfang Zhang, Ping Zhu, Qiang Gao, Dan Liu, Miaomiao Nie, Junmei Jia, Delin Qi","doi":"10.3389/fmicb.2025.1513401","DOIUrl":"10.3389/fmicb.2025.1513401","url":null,"abstract":"<p><strong>Background: </strong>The intestinal microbiota has co-evolved with the host to establish a stable and adaptive microbial community that is essential for maintaining host health and facilitating food digestion. Food selection is a critical factor influencing variations in gut microbial composition, shaping gut microbiome communities, and determining the ecological niches of fish.</p><p><strong>Methods: </strong>In this study, high-throughput amplicon sequencing of 16S rRNA and 18S rRNA was utilized to compare the dietary and gut microbial differences between <i>Schizopygopsis microcephalus</i> and <i>Ptychobarbus kaznakovi</i>, both collected from the same sites in the Tuotuo River and Tongtian River, which are tributaries of the Yangtze River. We compared the microbial community structure, diet composition, and diversity between the two fish species using various analytical methods, including LefSe, α-diversity and β-diversity analyses. Additionally, we constructed co-occurrence networks to determine their correlations.</p><p><strong>Results and discussion: </strong>The alpha diversity results indicated that <i>S. microcephalus</i> exhibited higher intestinal microbiota and feeding diversity compared to <i>P. kaznakovi</i>. Furthermore, the beta diversity results revealed significant differences in both intestinal microbiota and eukaryotic communities between the two species. The dominant bacterial phyla in both <i>S. microcephalus</i> and <i>P. kaznakovi</i> included Proteobacteria, Firmicutes, Actinobacteriota, Chloroflexi, and Verrucomicrobiota; however, Firmicutes was significantly more abundant in <i>P. kaznakovi</i> (<i>P</i> = 0.006), while Actinobacteriota was significantly higher (<i>P</i> = 0.019) in <i>S. microcephalus</i> at the phylum level. The primary food sources for <i>S. microcephalus</i> and <i>P. kaznakovi</i> were identified as Streptophyta (54.41%, 77.50%) and Cercozoa (8.67%, 1.94%), with Bacillariophyta (25.65%) was also the main food of constituting a major component of the diet for <i>S. microcephalus</i>. These differences suggested that <i>S. microcephalus</i> and <i>P. kaznakovi</i> occupy distinct dietary niches. To further explore the relationship between gut microbiota and feeding habits, we identified significant correlations between various food components and the gut microbial community through co-occurrence networks. This study enhances our understanding of the co-evolution and co-adaptation between host gut microbiota and feeding behaviors in sympatric fish species.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1513401"},"PeriodicalIF":4.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11935114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: The gut-liver axis: the main role of microbiome in liver diseases.","authors":"Giovanni Tarantino, Tiziana Di Renzo, Mauro Cataldi","doi":"10.3389/fmicb.2025.1567513","DOIUrl":"10.3389/fmicb.2025.1567513","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1567513"},"PeriodicalIF":4.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11935107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}