Frontiers in Microbiology最新文献

{"title":"Editorial: Common themes in drug resistance: from viruses to humans.","authors":"Mariana Carmen Chifiriuc, Coralia Bleotu","doi":"10.3389/fmicb.2025.1573798","DOIUrl":"10.3389/fmicb.2025.1573798","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1573798"},"PeriodicalIF":4.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the effect of anti-PIA/PNAG antibodies on biofilm formation in <i>Escherichia coli</i>.","authors":"Mina Shirmohammadpour, Mohammad Reza Mehrasbi, Nader Noshiranzadeh, Davoud Afshar, Kamyar Mansori, Bahman Mirzaei","doi":"10.3389/fmicb.2025.1552670","DOIUrl":"10.3389/fmicb.2025.1552670","url":null,"abstract":"<p><p>Polysaccharide Intercellular Adhesin (PIA), a surface polysaccharide produced by <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i>, is a compelling target for opsonic and protective antibodies against these bacteria. <i>Escherichia coli</i> has recently made an exopolysaccharide called poly-<i>β</i>(1,6)-<i>N</i>-acetylglucosamine (PNAG), biochemically indistinguishable from PIA. This study investigated the effect of antibodies generated against PNAG on biofilm formation and the opsonization activity of secreted antibodies in <i>Escherichia coli</i>. Following purification and structural confirmation of PIA polysaccharide from producing <i>Staphylococcus epidermidis</i>, the ability to inhibit biofilm and the function of secreted antibodies for the mentioned polysaccharide were evaluated using semi-quantitative methods in a mouse model. Subsequently, the opsonic activity of antibodies targeting <i>Escherichia coli</i> strain ATCC 25922 was evaluated. The extracted polysaccharide was confirmed using FTIR, NMR, and colorimetric methods, and the results showed that the purified PIA induced protective antibodies with 40.48% opsonization properties in <i>E. coli</i>. The sera of the PIA-immunized groups showed a significant increase in antibody production and protective IgG titer levels compared to the control group. Also, the antibodies produced showed a substantial difference in inhibiting biofilm production <i>in vitro</i> compared to non-immunized serum. Antibodies directed against PIA with a lethality of 40.48% showed a significant effect on the absence of biofilm formation in <i>E. coli</i>. Despite the opsonic properties of the antibodies for <i>E. coli</i>, the simultaneous impact of these antibodies on infections caused by <i>S. epidermidis</i> and <i>E. coli</i> may have a role that requires further investigation and studies in animal models.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1552670"},"PeriodicalIF":4.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of compound lactic acid bacteria on the quality and microbial diversity of alfalfa silage in saline-alkali soils.","authors":"Si-Yi Wang, Le Sun, Heng Jiang, Guo-Lin Yang, Zhen-Nan He, Yuan-Yuan Jing, Feng-Qin Gao","doi":"10.3389/fmicb.2024.1524296","DOIUrl":"10.3389/fmicb.2024.1524296","url":null,"abstract":"<p><p>With the rapid development of animal husbandry, forage resources are increasingly scarce. Improving the utilization rate of forage products and silage efficiency of planting is an urgent problem to be solved. This experiment used high moisture alfalfa at the budding stage with a water content of 71.4% from saline-alkali and non-saline-alkali soils as raw materials, setting up four experimental groups: non-saline-alkali alfalfa without additives (HNS-CK), non-saline-alkali alfalfa with compound lactic acid bacteria (<i>Lactobacillus</i> plantarum + <i>Lactobacillus</i> buchneri + <i>Pediococcus</i> pentosaceus + <i>Lactobacillus</i> kimchi, HNS-L4), saline-alkali alfalfa without additives (HS-CK), and saline-alkali alfalfa with compound lactic acid bacteria (HS-L4). After 60 days of silage, the quality and microbial diversity of the silage were tested. The results showed that the dry matter (DM) and lactic acid (LA) of the HNS-L4 group were significantly higher than those of the HS-L4 (<i>P</i> < 0.05), while there was no significant difference in crude protein (CP) between the HNS-L4 group and the HS-L4 (<i>P</i> < 0.05). The neutral detergent fiber (NDF), acid detergent fiber (ADF), pH of the HNS-L4 group were all lower than those of the HS-L4. The results of the microbial community showed that compared with the non-additives group, the Shannon index decreased and the Simpson index increased in the compound lactic acid bacteria group, indicating a significant reduction in microbial diversity in the silage environment (<i>P</i> < 0.05). The dominant bacteria in the HNS-CK and HS-CK groups were <i>Enterobacteriaceae</i>, while the dominant bacteria in the HNS-L4 and HS-L4 groups were <i>Lactobacillus</i>. At the phylum level, the dominant bacteria in alfalfa after lactic acid bacteria treatment were Firmicutes, which were significantly higher than the control group (<i>P</i> < 0.05). Therefore, compound lactic acid bacteria can improve the quality of alfalfa silage in both saline alkali and non-saline-alkali soils, with saline-alkali soils being better than non-saline-alkali soils, and both can reduce microbial diversity.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1524296"},"PeriodicalIF":4.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A process-based dynamic model for succicinic acid production by <i>Actinobacillus succinogenes</i>: regulatory role of ATP/ADP balance.","authors":"Emiliano Salucci, Fabrizio Cartenì, Francesco Giannino, Elisabetta de Alteriis, Francesca Raganati, Stefano Mazzoleni","doi":"10.3389/fmicb.2025.1512982","DOIUrl":"10.3389/fmicb.2025.1512982","url":null,"abstract":"<p><strong>Introduction: </strong>Succinic acid is an important chemical compound for biotechnological productions, being used as a basic platform to produce many industrial products in major business applications. It can be produced as fermentation end-product of anaerobic metabolism of different bacterial species, among which <i>Actinobacillus succinogenes</i> is largely used. Modeling microbial metabolic processes in controlled bioreactor systems is recognized as a useful tool to optimize growth conditions aimed at maximizing yield.</p><p><strong>Methods: </strong>A novel model is presented based on System Dynamics approach in which the maintenance of the ATP/ADP balance is introduced as a key regulatory process of <i>A. succinogenes</i> metabolism.</p><p><strong>Results and discussion: </strong>Model simulations accurately reproduce microbial growth and succinic acid production in anaerobic batch cultures at different initial glucose concentrations. Results reveal that the main limitations to maximal succinic acid production are glucose uptake restrictions and energy homeostasis costs (ATP/ADP balance) of the microbial population. The process-based modeling approach effectively describes the main metabolic processes and their regulation, providing a useful tool to define working conditions and overcome the criticalities of the SA fermentation process.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1512982"},"PeriodicalIF":4.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Bacillus amyloliquefaciens</i>- modified clay for controlling harmful algal blooms in eutrophic aquaculture ponds.","authors":"Zaixing Wu, Zhiming Yu, Xiuxian Song, Kaiqin Jiang, Xihua Cao, Yongquan Yuan","doi":"10.3389/fmicb.2025.1550905","DOIUrl":"10.3389/fmicb.2025.1550905","url":null,"abstract":"<p><strong>Introduction: </strong><i>Bacillus amyloliquefaciens</i> is a commonly used probiotic microecological regulator in aquaculture water with the ability to inhibit harmful algae blooms (HABs) and improve the health status of aquaculture ecosystem. Modified clay has been widely applied in the field for eliminating HABs.</p><p><strong>Methods: </strong>In this study, clay particles are used as carriers and to provide a microenvironment for microbial fermentation, yielding a microbial modified clay product with high efficiency for eliminating typical HAB organisms. Methods are developed for the fermentation of microorganisms and clay particles and also for subsequent preparation of a dry powder, which stably produce microbial modified clay in dry powder form for HAB elimination.</p><p><strong>Results: </strong>The results showed that the obtained microbial modified clay has a stable bacterial content of more than 10⁹ cfu/mL, and the removal rate of the HAB organism <i>Heterosigma akashiwo</i> equivalent to that obtained with other HAB removal materials at the same dosage, indicating good potential for HAB removal. The removal rate of <i>Heterosigma akashiwo</i> at a dosage of 0.1 g/L microbial modified clay exceeded 90%.</p><p><strong>Discussion: </strong>By combining two natural and commonly used material, both the function of emergency flocculation disposal (when HAB outbreaks) and long-term ecological regulation (to maintain a healthier phytoplankton community structure through specific algicidal effect) were achieved, resulting in a 1 + 1 > 2 effect when applied in the eutrophic aquaculture environment. Also, this exploratory study with the unique combination of microbial fermentation and clay modification preliminarily provides an important scientific basis for promoting the large-scale application of microbial modified clay in the field of HAB control, especially in the eutrophic aquaculture ecosystems. Also, extensive ecological assessment before field application is still required, such like the scientific support of environmental criteria, the safety to other aquaculture organisms and the ecological effect of the whole aquaculture ecosystem.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1550905"},"PeriodicalIF":4.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity and mechanism of Sodium houttuyfonate against heteroresistant <i>Pseudomonas aeruginosa</i>.","authors":"Zhihong Li, Tongtong Zhang, Ziqi Wang, Shuqiang Huang, Cuiyu Tan, Dan Wang, Xiaojun Yuan, Lingqing Xu","doi":"10.3389/fmicb.2025.1523182","DOIUrl":"10.3389/fmicb.2025.1523182","url":null,"abstract":"<p><strong>Background: </strong><i>Pseudomonas aeruginosa</i> is a common gram-negative opportunistic pathogen that is now commonly treated with carbapenems, such as Meropenem. However, the increasing rate of emergence of heteroresistant strains poses a therapeutic challenge. Therefore, we examined the antibacterial activity of Sodium houttuyfonate (SH, a compound derived from <i>Houttuynia cordata</i>) in combination with Meropenem (MEM) against heteroresistant <i>Pseudomonas aeruginosa</i> and investigated the mechanism of Sodium houttuyfonate.</p><p><strong>Methods: </strong>Heteroresistant <i>Pseudomonas aeruginosa</i> was used as the experimental strain for the study and the combined action activity of the two drugs was inves-tigated by determining the Minimum Inhibitory Concentration (MIC), Fractional Inhibitory Concentration Index (FICI), and time killing curves. Also the effect of Sodium houttuyfonate on biofilm as well as bacterial swimming motility assay was investigated by crystal violet staining of bacterial biofilm, microanalysis of biofilm, bacterial swimming motility assay, quantitative real-time PCR (qRT-PCR) and population sensing related virulence factors.</p><p><strong>Results: </strong>For the screened experimental strains, the MIC of SH was 4,000 μg/ml; the FICI of both drugs on the four experimental strains was ≤0.5, which showed a synergistic effect. When SH ≥ 250 μg/ml, it was able to effectively inhibit bacterial biofilm formation as well as swimming ability compared with the blank control group. In the qRT-PCR experiment, the expression of biofilm formation-related genes (<i>pslA</i>, <i>pelA</i>, <i>aglD</i>, <i>lasI</i>, <i>lasR</i>, and <i>rhlA</i>) and swimming ability-related genes (<i>fliC</i>, <i>pilZ</i>, and <i>pilA</i>) were decreased in the SH-treated group, compared with the blank control group.</p><p><strong>Conclusion: </strong>Our study demonstrated that Sodium houttuyfonate and Meropenem exhibited synergistic inhibition against heteroresistant <i>Pseudomonas aeruginosa</i>, and that Sodium houttuyfonate may achieve its inhibitory effect by inhibiting bacterial biofilm formation, inhibiting motility, and down-regulating related genes.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1523182"},"PeriodicalIF":4.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Complementary effects of virus population are required for efficient virus infection.","authors":"Yuechao Sun, Yu Zhang, Xiaobo Zhang","doi":"10.3389/fmicb.2025.1535353","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1535353","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fmicb.2022.877702.].</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1535353"},"PeriodicalIF":4.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Spartina alterniflora</i> modifies the native arbuscular mycorrhizal fungal community in coastal ecosystem.","authors":"Yuxin Jiang, Meng Li, Xiaohong Guo","doi":"10.3389/fmicb.2025.1544111","DOIUrl":"10.3389/fmicb.2025.1544111","url":null,"abstract":"<p><p>The effect of invasive plants is mediated by their interactions with microbial communities. However, it is still uncertain how <i>Spartina alterniflora</i> impacts the arbuscular mycorrhizal fungi (AMF) community within the native rhizosphere what the resulting AMF differences are associated with. Here, we investigated what kind of AMF communities are formed in the roots of <i>S. alterniflora</i> to distinguish it from native plants such as <i>Suaeda salsa, Phragmites australis</i>, and <i>Tamarix chinensis</i> by analyzing the AMF communities and the associations with selected environmental factors. The dynamics of AM fungal communities are linked to plant-soil systems. The AMF communities of <i>S. alterniflora</i> and native vegetation demonstrated notable differences in composition, diversity, and symbiotic networks. Significantly higher ω, Ec, AN, AP, and AK were observed in <i>S. alterniflora</i>-invaded soils. Although plant rhizosphere AMF responded to soil environmental factors, AN and AP were highly explanatory environmental factors driving AMF community characteristics during <i>S. alterniflora</i> expansion, while increased soil P and N availability may be involved in shaping AMF community characteristics in <i>S. alterniflora</i>. Our findings can provide complementary evidence-based solutions for defending against invasive plants and mitigating their impacts, as well as protecting coastal ecosystems.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1544111"},"PeriodicalIF":4.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global characterization of GH3 family glycoside hydrolase genes in <i>Fusarium verticillioides</i> and functional analysis of <i>FvGH3-6</i>.","authors":"Xiaojie Zhang, Pengliang Duan, Shaoqi Shi, Manli Sun, Ning Liu, Zhiyan Cao, Jingao Dong","doi":"10.3389/fmicb.2025.1543210","DOIUrl":"10.3389/fmicb.2025.1543210","url":null,"abstract":"<p><p>To clarify the roles of glycoside hydrolase 3 (GH3) family genes in the growth, development, and pathogenicity of <i>Fusarium verticillioides</i>, GH3 family genes were identified in the genome by bioinformatics software, and their expression levels in the infection process of <i>F. verticillioides</i> were analyzed using transcriptome data. The <i>FvGH3-6</i> gene was knocked out and complemented via genetic transformation to explore the role of <i>F. verticillioides</i>. The results demonstrated that a total of 19 GH3 family genes were identified in the genome of <i>F. verticillioides</i>, which were located on 11 chromosomes, encoding amino acids ranging from 559 to 1,034, with relative molecular weights between 61.20 and 113.97 kDa, and containing 1-6 exons. Transcriptome data indicated that during the infection of maize kernels by <i>F. verticillioides</i>, the expression of nine genes, including <i>FvGH3-6</i>, was upregulated at different stages. Knockout of the <i>FvGH3-6</i> gene did not impact the mycelial growth rate of <i>F. verticillioides</i> but reduced the sporulation ability. Compared with the wild type, the pathogenicity of <i>FvGH3-6</i> knockout mutants towards maize grains and stems was weakened. The above results suggest that the glycoside hydrolase gene family participates in the pathogenicity of <i>F. verticillioides</i>, and the <i>FvGH3-6</i> gene plays a significant role in the conidia production and pathogenicity of <i>F. verticillioides</i>.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1543210"},"PeriodicalIF":4.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic value of the combined test of leukocytes in urine and TB-DOT and T-SPOT.TB in blood for urinary tuberculosis.","authors":"Yanyan Li, Yachun Wang, Lukuan Wei, Wei Wang","doi":"10.3389/fmicb.2025.1535490","DOIUrl":"10.3389/fmicb.2025.1535490","url":null,"abstract":"<p><strong>Background: </strong>This study aims to evaluate the clinical utility of routine urinary tests and renal function assessments, as well as the Tuberculosis antibody test (TB-DOT) and T-cell spot tests for TB infection (T-SPOT.TB), either individually or in combination, for diagnosing urinary tuberculosis (UTB).</p><p><strong>Methods: </strong>We conducted a retrospective analysis of urinary routine tests, renal function tests, TB-DOT, and T-SPOT.TB-administered alone or in combination-in 95 patients suspected of having UTB from January 2020 to December 2022 at our institution.</p><p><strong>Results: </strong>Significant differences were observed in the levels or positivity of white blood cells (WBC), red blood cells (RBC), creatinine (Crea), TB-DOT, and T-SPOT.TB between the UTB group and the non-UTB group (<i>P</i> < 0.05). Among the individual tests, T-SPOT.TB exhibited the highest specificity and positive predictive value (PPV), while WBC demonstrated the highest area under the curve (AUC). Both TB-DOT and RBC showed relatively good sensitivity. Additionally, WBC levels correlated with both TB-DOT and T-SPOT.TB results. The combination of WBC, TB-DOT, and T-SPOT.TB provided the best sensitivity, negative predictive value (NPV), and AUC when evaluated in parallel with the other tests.</p><p><strong>Conclusion: </strong>For the early identification of UTB, the sensitivity of T-SPOT.TB and TB-DOT tests is superior to that of routine urinary and renal function tests. The parallel combination of WBC, TB-DOT, and T-SPOT.TB offers enhanced diagnostic efficacy for UTB, facilitating rapid clinical diagnosis.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1535490"},"PeriodicalIF":4.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}